Digital Commons Network^™

Investigating The Mechanics And Chemistry Of Sickle Polish Development, Justin Jared Dubois

Graduate Theses and Dissertations

This series of experiments is designed to understand the development and chemistry of sickle gloss. Sickles are common in the archaeological record and have long been studied for their eponymous “sickle gloss”. There is debate as to how this gloss is formed. Five experimental sickles containing flint and novaculite blades were used to harvest high and low moisture content rye and common fescue and associated field weeds. The differences in the development of sickle gloss were examined. High moisture content plants create thick, smooth fields of polish with undulating, billowing margins at a higher developmental speed. Low moisture content plants …

Investigating Molecular Mechanisms Behind Bacterial Chondronecrosis With Osteomyelitis (Bco) Pathogenesis In Modern Broilers, Alison Ramser

Graduate Theses and Dissertations

Bacterial chondronecrosis with osteomyelitis (BCO), a leading cause of lameness in broiler chickens, is characterized by infection, inflammation, and bone attrition. There are currently no effective treatments and positive diagnosis is only possible through necropsy evaluations. Lameness is also a rising animal welfare and economic concern, making prevention and detection of BCO all the more critical. These challenges are exacerbated by a lack of mechanistic understanding of BCO’s etiology. The question I asked during my dissertation was how bacteria induce bone attrition in BCO pathology. My research has shown that mitochondrial dysfunction is characteristic of BCO conditions along with autophagy …

An Engineering And Economic Analysis Of The Implementation Of The Carbo Rock-It, Lydia Huck

Graduate Theses and Dissertations

The patented Carbo Rock-It system was developed to carbonate beverages for the craft brewing industry with the goal of reducing time, cost, and environmental impacts of the carbonation process. This research field tests, models, analyzes and optimizes the engineering and economic performance of a commercial-scale Carbo Rock-It placed in production at an operating brewery (Core Brewing and Distilling Co., Springdale, AR). Field tests indicated that the Carbo Rock-It was able to successfully carbonate twelve 120 BBL batches of Scarlet Letter Seltzer to 3.0 vol/vol while meeting all beverage quality and safety requirements. The Carbo Rock-It was able to reduce carbonation …

Studying Acetylation Of Aconitase Isozymes By Genetic Code Expansion, Jessica Araujo

Graduate Theses and Dissertations

The tricarboxylic acid (TCA) cycle is a very important, centrally located, energy-producing pathway that connects numerous other metabolic and regulatory pathways. Enzymes of this cycle have been more recently implicated in various cancers and neurometabolic disorders, however, the exact mechanism by which this happens becomes quite complex when considering the potential modification of these enzymes and the presence of multiple forms of the enzymes and therefore there is much to be studied in this area.

Aconitase has become a recent enzyme of interest as its substrate, citrate, has been found to play a major role in many vital processes within …

Essential Amino Acid (Eaa) Regulation Of Skeletal Muscle Protein Turnover With Age, Mary Komp

Graduate Theses and Dissertations

Skeletal muscle (SM) is vital for both long term health and quality of life. Recent research suggests an increase in catabolic signals with age triggers pathologic conditions, such as sarcopenia. Although results from in vitro studies model how EAA can regulate muscle protein synthesis (MPS), the relevance of these models to muscle protein breakdown (MPB) and the presence of physiological EAA concentrations remains to be established. Therefore, the objective of this study was to determine the effects of a low, normal, and supra physiological dose of EAA (0.2, 1.0, and 3.0 x EAA) in a young (passages 2-10) and aging …

Ghosts And Ethics In The Early Works Of James Joyce, Michael Menase

Graduate Theses and Dissertations

In the last 20 years, critics have contributed new insights into the character development, overall messages, themes, and other literary aspects of James Joyce’s works by focusing on their ethical implications. By following an intertextual method and by performing close readings of Joyce’s texts, I try to fill a gap in the scholarly literature by adding a related focus on ghosts to the conversation about Joyce’s ethics and those of his characters. Focusing on Dubliners and A Portrait of the Artist as a Young Man, I hope to show that the ghosts in Joyce’s corpus motivate characters and readers to …

Reconstructing Bison And Mammoth Migration During The Late Pleistocene And Early Holocene Of Central Texas Using Strontium Isotopes, Joshua John Porter

Graduate Theses and Dissertations

During the Late Pleistocene (LP; past 130,000 years), over two-thirds of large mammal (>45kg) species went extinct globally. While the role of humans is hotly debated, the effect of these extinctions is growing clearer; the extinctions resulted in widespread and lasting faunal community reorganization. However, the impact of these extinctions on dietary and migratory behavior within faunal communities is unknown. Our study examines the impact of the megafaunal extinctions on the dietary and migratory behavior of surviving Bison individuals in Texas using carbon, oxygen, and strontium isotopes. Strontium isotopes are incorporated into mammalian enamel during their tooth development and …

Examining The Effects Of Oral Contraceptive Use On Thermoregulation, Whitley C. Atkins

Graduate Theses and Dissertations

Purpose: The purpose of this investigation was to evaluate the effects of combined (estradiol and progestin) monophasic oral contraceptive pill (OCP) use on thermoregulation. Further, we sought to evaluate OCP use on acute rehydration post-exercise in the heat using recommended rehydration guidelines. Methods: Twelve healthy, aerobically trained (VO2peak = 47.8 ± 4.7 mL/kg/min), long term female oral contraceptive users completed a familiarization trial and two experimental days separated by ≥ 7 days. The two experimental trials were identical except for the hormone dosing phase. One trial was completed during the third week of active pill dosing (ACT) and one during …

Macronutrient Availability Shapes Host Response To Infection And Feeding Behavior, Weston Gerald Perrine

Graduate Theses and Dissertations

Macronutrients in the diet are vital to the physiological processes necessary for an organism to effectively clear a pathogen. Diet can be important to a host’s susceptibility to infection and severity of pathology, though results can vary across host-pathogen systems (Sen et al. 2016). Manipulating the ratio of specific macronutrients in the diet is an effective method to begin understanding how individual macronutrients, rather than food types, have on immune responses. Using an avian host-pathogen system, I explored the effects of dietary macronutrient composition, specifically lipid and protein content, on disease pathology and behavior of canaries (Serinus canaria) infected with …

Post-Obergefell V. Hodges: How Lgbt Contact Can Alter Public Lgbt Policy Positions In The U.S. And Arkansas, Briana Huett

Graduate Theses and Dissertations

The Contact Theory (CT) of attitudinal change has utilized to understand perceptions of minority-group members and the policies that surround them since the 1950s. It has further been used to specifically examine how we form our opinions of LGBT-identifying individuals, the LGBT community, and LGBT policies more generally. However, further evidence is still needed from the CT literature surrounding how this form of contact interacts with individuals’ social identities to determine and alter their LGBT policy positions, how the level of contact with LGBT persons might have differing effects on these positions, and whether LGBT contact holds the same effects …

A Mixed Methods Approach To Context Specific Curriculum Design For Very Small And Small Strawberry Growers In The Southeastern United States, Thomas A. Yeargin

Graduate Theses and Dissertations

Outbreaks of foodborne illness due to fresh produce are a continued threat to both the publichealth and the economy in the United States. Though there are many factors which influence the perpetuation of foodborne pathogens, the inability of the food industry to curtail this issue indicates systemic failure of interventions aimed at improving food safety practices. In this dissertation, we detail the efforts made over the past few decades to provide training to food producers and food handlers as well as recommendations that have been made for improvement based on these studies. By borrowing from more advanced fields of study …

The Effects Of Metronomic And Maximum-Tolerated Dose Chemotherapy In Colorectal Cancer Angiogenesis: A Combined Approach Using Endoscopic Diffuse Reflectance Spectroscopy And Mrna Expression, Ariel Isaac Mundo Ortiz

Graduate Theses and Dissertations

Colorectal cancer (CRC) continues to be one of the most incident and deadliest types of cancer worldwide. Chemotherapy has proven effective to reduce tumor burden for CRC patients, but there are several disadvantages associated with the use of mainstay maximtolerated dose (MTD) chemotherapeutic strategies. Metronomic chemotherapy (MET) has been developed as an alternative that addresses the shortcomings of maximum-tolerated dose chemotherapy but so far its effectiveness as a neoadjuvant strategy for CRC has not been explored.

This dissertation uses a combined optics and molecular biology approach (using diffuse reflectance spectroscopy and mRNA expression) to study the changes in angiogenesis and …

Characterization Of The Influence Of A Small Molecule Inhibitor On Ras-Related Proteins Interactions, Emilio Duverna

Graduate Theses and Dissertations

The Ras superfamily of small G proteins are involved in cell-signaling processes that, if not regulated, may lead to cell multiplication, apoptosis inhibition, and tumorigenesis. They function as molecular switches, which through GTP/GDP exchange cycle, switch on or off cellular activities. Overexpression and/or hyperactivity of these proteins have been linked to many diseases including various cancers. CDC42, a member of the Rho subfamily of the Ras superfamily of small G proteins, participates in the regulation of many cellular processes including cell adhesion, mitosis, and cytoskeletal rearrangements. CDC42 binds to and activates many effector proteins including CDC42-activated kinase (ACK). Abnormal activities …

Diffusive Dynamics Of Biological Macromolecules In The Cytoplasm Of Live Bacteria, Asmaa Sadoon

Graduate Theses and Dissertations

Bacteria are small organisms that play important roles in our bodies and in maintaining our surrounding environment. However, bacterial infections are considered as one of the health diseases that threatens the lives of humans. Resistance of bacteria to antibiotics has made treating bacterial infections more challenging. With the emergence of many developed optical techniques, it has become possible to study various biological mechanisms of molecules inside these small organisms with high resolution and precise localization. In this research, single particle tracking photoactivation localization microscopy (sptPALM) technique was used to investigate the dynamics of fluorescent histonelike nucleoid-structuring proteins (H-NS) in live …

Cyanobacterial And Microcystin Response To Nutrient Additions At Lake Fayetteville Throughout The 2021 Growing Season, Lillie Haddock

Graduate Theses and Dissertations

Harmful Algal Blooms (HABs) are becoming a global concern due to their increasing distribution, frequency, intensity, and the occurrence of toxins. While it is known that eutrophication influences algal blooms, there is less known about what triggers these HABs to produce toxins, especially microcystin. In this study, we conducted 21 community bioassays at Lake Fayetteville, a hypereutrophic reservoir in Fayetteville, Arkansas, from April-November 2021 to examine how the addition of phosphorous and nitrogen influence cyanobacteria concentrations, microcystin concentrations, and microcystin toxin production. These experiments included a control, nitrogen (1.0 mg/L as KNO3), low phosphorus (0.025 mg/L as K2HPO4), high phosphorus …

Multi-Trophic Biodiversity Increases With Increasing Structural Complexity Of Forest Canopy, Ayanna St. Rose

Graduate Theses and Dissertations

Understanding the effects of forest canopy structural complexity on multi-trophic diversity is critical for conserving biodiversity and managing land sustainably. But multi-trophic diversity is often ignored when making decisions about land management due to lack of cost- and time-effective methods to evaluate it. Here, we explored a new method based on widely available remote sensing data to quantify canopy structural complexity and its relationships with multi-trophic biodiversity at landscape scale using 32 forested sites of the National Ecological Observatory Network. We investigated the influence of vertical and horizontal structural complexity of forest canopy on multi-trophic (primary producers, herbivores (beetles), omnivores …

Development Of A Long-Read Sequencing Protocol To Assess The Precision And Efficacy Of Gene Editing For Duchenne Muscular Dystrophy, Landon Andrew Burcham

Graduate Theses and Dissertations

This work establishes a method for assessing on-target precision due to CRISPR-Cas9 gene editing, especially within the context of exon skipping therapy for Duchenne Muscular Dystrophy. The proposed method utilizes an Oxford nanopore long-read sequencing approach to sequence amplified regions of DNA that have been edited using CRISPR-Cas9. NIH3T3 and C2C12 cell lines were treated with a dual-guide CRISPR-Cas9 system, that targets and deletes exon 23 from the DMD gene in mouse samples. Deletion PCR revealed deletion of exon 23 in both DNA and cDNA samples. Additionally, sequencing using Oxford Nanopore revealed targeted exon 23 deletion as the most prevalent …

Tooth Crown Morphology And Testosterone Transfer In Female Members Of Opposite-Sex Dizygotic Twin Pairs, Randall Feezell

Graduate Theses and Dissertations

The twin testosterone transfer (TTT) hypothesis posits that females gestated with male co-twins develop more masculine phenotypes due to in-utero androgen exposure. Research has shown co-gestational effects to be associated with increased deciduous and permanent tooth size in females belonging to opposite-sex dizygotic twin pairs (OSF) as compared with females belonging to same-sex monozygotic (MZF) and dizygotic (SSF) twin pairs and female siblings. This study evaluates whether the TTT hypothesis explains patterns of dental morphological variation, namely differences between OSF and other females (SSF, MZF, female siblings) in a contemporary sample that includes both deciduous and permanent data. This work …

To The Window, To The Wall: Improving Environmental Monitoring Protocols, Sarah Jones

Graduate Theses and Dissertations

Detecting microorganisms on environmental surfaces via an environmental monitoring (EM) program is part of a preventive food safety culture. Environmental monitoring should 1) verify that food safety plans are reducing cross-contamination risk from surfaces to food, 2) pinpoint microbial niches, and 3) prevent the transmission of pathogens. Environmental monitoring programs utilize EM tools, such as sponges, to sample food contact and non-food contact surfaces. However, EM tool selection is determined by the individual food firm. This dissertation evaluated and characterized factors influencing EM program effectiveness in the food industry. Specifically, this dissertation focuses on the release of microorganisms from EM …

The Impact Of Dietary Protein Supplementation As Part Of A Time Restricted Feeding Eating Pattern On Sleep, Mood, And Body Composition In Adults With Overweight Or Obesity, Rebecca L. Bowie

Graduate Theses and Dissertations

Obesity in the United States continues to increase, and effective, long-term weight loss strategies are limited. Time restricted feeding (TRF) is a dietary intervention that has potential to serve as an effective long-term weight loss strategy, however more data is needed to establish the effectiveness of a TRF eating pattern on obesity and its related health outcomes. To our knowledge, manipulation of macronutrient composition during TRF intervention and the effect of TRF on sleep and mood have not been studied. Therefore, the objective of this study was to determine the effect of protein supplementation during TRF on mood, sleep, and …

Imagining Communicative Success: How Imagined Interactions Affect Conflict Management In Romantic Relationships, Melinda Shaw

Graduate Theses and Dissertations

Currently, there are no bodies of research that examine the relationship between imagined interactions, relational maintenance, conflict management, and gender identity. Previous research has primarily relied on quantitatively measuring the functionality of imagined interactions and generating an understanding as to why individuals use them. However, this study qualitatively examined the connection between these concepts, specifically how gender identity affects how individuals use imagined interactions to manage conflict with their partner and maintain their relationship. Fifteen participants who are currently in long-term relationships were interviewed and then required to complete the Thomas-Kilmann (1974) conflict management mode survey. Afterward, the responses were …

Preparation Of Proteins And Peptides From Heat-Stabilized Defatted Rice Bran Via Solid State Fermentation And Investigating For Skin Health, Ali A. Bisly

Graduate Theses and Dissertations

Heat-stabilized defatted rice bran (HDRB) is an abundant and inexpensive agro-industrial by-product of rice milling and rice oil extraction process. Furthermore, HDRB is a potential source of proteins. However, the direct protein extraction has low yield of protein due to the impact of heat stabilization and oil extraction process which made the HDRB proteins bound and interact with other components. This research is based on using solid-state fermentation (SSF), which is a natural and cost-effective approach to facilitate protein extraction from HDRB using Bacillus subtilis (natto) Takahashi as a microorganism.

Response surface methodology (RSM) analysis was used with Box-Behnken design …

Managing Stress Tolerance On Warm-Season Putting Greens In The Transition Zone, Thomas Edward Walton

Graduate Theses and Dissertations

The use of warm-season putting greens in the transition zone has increased in recent years. Ultradwarf bermudagrass (Cynodon dactylon (L.) Pers x C. transvaalensis Burtt-Davy) is the most prevalent warm-season putting green selection in the transition zone, however, newly developed greens-type zoysiagrass (Zoysia spp.) cultivars represent another potential selection for golf courses. The two major limitations of warm-season grasses in the transition zone are a general lack of cold- and shade-tolerance. Protective covers are essential to protect ultradwarf bermudagrass putting greens in the winter months. Unfortunately, golf courses can still experience winterkill underneath protective covers. Two field trials were conducted …

Photoassisted Nanoscale Memory Resistors, Amir Shariffar

Graduate Theses and Dissertations

Memristors or memory resistors are promising two-terminal devices, which have the potential to revolutionize current electronic memory technologies. Memristors have been extensively investigated and reported to be practical devices, although they still suffer from poor stability, low retention time, and laborious fabrication processes.

The primary aim of this project was to achieve a device structure of quantum dots or thin films to address a fundamental challenge of unstable resistive switching behavior in memristors. Moreover, we aimed to investigate the effects of light illumination in terms of intensity and wavelength on the performance of the fabricated memristor. The parameters such as …

Making Connections: Understanding How Social Interaction Patterns Influence Work-Related Outcomes For Individuals With Disabilities, Christine Marie Manno

Graduate Theses and Dissertations

In the United States, approximately eight million individuals with disabilities are active in the workforce (Erickson et al., 2008). These individuals seek employment that allows for full use of their education, experience, and abilities, and provides growth and development opportunities (Ali et al., 2011). Despite protective legislation and inclusive organizational policies, individuals with disabilities are at least twice as likely as individuals without disabilities to experience underemployment and reduced access to advancement opportunities (Schur et al., 2009). Evidence suggests that some of these issues may be related to social interaction patterns. It appears that individuals with disabilities have a strong …

“Implacement”: The Importance Of Place And Community In Schooling, Emily Coady

Graduate Theses and Dissertations

This dissertation consists of three chapters exploring my overarching research question: How does sense of place shape a teacher’s orientation to their profession? My first chapter empirically answers the question: Is there a relationship between place attachment and measured aspects of teacher quality? In an attempt to qualify the strength of bond between a teacher and his or her community, I adapted a place attachment instrument developed by Raymond, Brown, and Weber (2010). Each teacher is given a place attachment score informed by their responses on the 21-item survey. Next, I correlated these scores to other aspects of teacher quality …

How Nonbinary College Students At Southern Universities Explore Identity Through Gender Expression, Charles Jesse Thompson

Graduate Theses and Dissertations

As the number of young people identifying as nonbinary continues to increase (Davis, 2018), our need, as educators, to understand their experiences on college campuses also increases. Using narrative inquiry and photovoice, framed by critical queer theory and genderism, this examined both institutionalized and socially constructed expectations and influences that affect the development of nonbinary gender expression and identity for the college students by addressing the research question: What are the experiences of nonbinary college students in the Southern United States in exploring their gender expression at research universities? The five major themes that emerged from this study were as …

Increasing Active Learning And Achievement In A Large Lecture Calculus Class Through A Flipped Classroom Model, Kimberly King

Graduate Theses and Dissertations

University Calculus I courses serve as a means of access into high demand STEM fields and large lecture style passive calculus courses can be difficult for students. A mixed methods research design was used to compare a flipped instructional approach to a traditional lecture approach in large section Calculus I courses. The flipped lecture model required students to view videos of calculus instruction that included embedded quiz questions to allow for problem solving explorations during face-to-face class time. The traditional format included content from the video and limited time for additional problem solving. A professor with prior experience teaching Calculus …

Examination Of The Colorful Semantic Approach Via Telepractice For Children Who Are Deaf Or Hard Of Hearing, Samadhi Pusuba Devayalage

Graduate Theses and Dissertations

Introduction: Hearing loss, deafness or hard of hearing are considered to be the inabilityof perceiving sounds beyond 20 dB. Due to a direct impact of a hearing loss, a developing brain undergoes difficulties in acquiring age-appropriate syntax and speech sounds. As a result, children with hearing loss present language, speech, and literacy disabilities. The current study discusses the efficacy of the colorful semantics approach in order to see its impact on sentence structure development. Methodology: A single subject withdrawal experimental study conducted following ABAB model. Two participants (6 years and 10 years) were recruited to the study following an inclusion …

Implementing An Exercise Referral Scheme For Promoting Physical Activity In University Mental Health Care, Bryce Daniels

Graduate Theses and Dissertations

Introduction. Mental health symptoms and conditions are prevalent and increasing on college campuses with anxiety and depression having the highest increases in prevalence. Many college students are also physically inactive which is problematic as physical activity benefits mental health. Exercise referral schemes (ERS) can promote physical activity, though no studies have investigated the implementation of an ERS within a college counseling center. Purpose. The purpose of this dissertation was to investigate the implementation of an ERS for promoting physical activity in university mental health care. Methods. Study 1 used a qualitative descriptive design of 14 certified counselors to explore perceptions …