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Effect Of Dietary Tryptophan Supplementation And Heat Stress On Pasture Broiler Welfare And Performance, Jaelen Matthew Cherry
Effect Of Dietary Tryptophan Supplementation And Heat Stress On Pasture Broiler Welfare And Performance, Jaelen Matthew Cherry
Graduate Theses and Dissertations
Pasture-raised broiler chickens face temperature extremes that can threaten welfare. In the study, we evaluated whether a 0.26 % tryptophan-supplemented diet (T) improved heat stress (HS) resilience in Ross-708 broilers raised on pasture. At D14, birds began the T or remained on the control diet (C) and were moved outdoors. On D20 and 41, half of each group underwent six-hour HS; the remainder stayed under thermoneutral (TN) conditions. Weekly body weight, feed intake on D20 and D41 were recorded, and fear was measured using the Tonic Immobility (TI) test. Neither diet nor HS affected body weight. However, tryptophan supplementation resulted …
Effect Of Marination And Storage Time On The Properties And Meat Quality Of Broiler Breast, Mayra Mabel Herrera Rivera
Effect Of Marination And Storage Time On The Properties And Meat Quality Of Broiler Breast, Mayra Mabel Herrera Rivera
Graduate Theses and Dissertations
Abstract The poultry industry has implemented numerous methods, as marination and aging, to improve meat quality and satisfy consumer demands. Three experiments were developed to assess the effects of vacuum tumble marination and aging on the proteins of chicken breast. These experiments utilized 0.75 % sodium chloride, 0.45 % phosphate, and water in a marinade. In experiment one, 270 fillets were allocated into six treatment groups, each comprising 45 fillets. Six days of storage time were used, marinating and storing the fillets in each group at a temperature of 4°C. Following each day of storage period, the fillets underwent a …
Minor League: The Journey To The Show, Collin Anderson
Minor League: The Journey To The Show, Collin Anderson
Graduate Theses and Dissertations
When thinking about the path an athlete takes to get to the major leagues, many think of the same process: High School, College, Professional. However, the professional level includes is more than just the MLB or NBA that people would assume. Almost all professional U.S. sports leagues have a minor league system associated with them that nearly all players pass through at one point or another. Through the lens of a documentary, this portion in an athlete’s journey will be spotlighted to show what these leagues do for them, how they help them improve, and how they prepare them to …
Effects Of Flavonoids On Reducing Colon Cancer Risk Via Macrophage Polarization, Gracien Jules
Effects Of Flavonoids On Reducing Colon Cancer Risk Via Macrophage Polarization, Gracien Jules
Graduate Theses and Dissertations
Colorectal cancer is one of the most fatal diseases in the United States. Chronic tissue inflammation can cause cell proliferation, which leads to tumor formation and cancer development. Macrophages are responsible for maintaining tissue homeostasis and for inflammatory responses. M1 macrophages exhibit proinflammatory responses while M2 macrophages exhibit anti-inflammatory responses. Imbalance between M1 and M2 macrophages is closely related to inflammation-associated disease development. The anti-inflammatory effects of apiaceous vegetable constituents, specifically flavonoids, have been studied for their preventative effects towards reducing disease risk. However, sufficient evidence is lacking on the mechanisms by which flavonoids impact M1/M2 macrophage polarization. RAW 264.7 …
Performance And Feasibility Of Pasture Rangeland And Forage Insurance In The Delta Region, Walker Bradley Davis
Performance And Feasibility Of Pasture Rangeland And Forage Insurance In The Delta Region, Walker Bradley Davis
Graduate Theses and Dissertations
Despite being available nationwide since 2016, enrollment in Pasture, Rangeland, and Forage (PRF) insurance remains low in many regions of the country. The program's performance also presents regional discrepancies, with loss ratios varying significantly. Studies that have simplified the complexity of interval selection for producers have been shown to positively affect PRF enrollment. Such studies remain limited in several forage-producing areas. This study aims to address the lack of information in Arkansas specifically. Arkansas has among the nation's lowest participation rates and loss ratios. Low loss ratios signal that participants pay more in premiums than received in indemnities and may …
Polymer-Enhanced Nanopore Sensing, Eugene Gyasi Agyemang
Polymer-Enhanced Nanopore Sensing, Eugene Gyasi Agyemang
Graduate Theses and Dissertations
The resistive pulse technique characterizes single analytes by detecting fluctuations in the ion current as they pass through a narrow orifice connecting two electrolyte-filled chambers, each containing a single electrode. The amplitude, shape, frequency, and duration of these fluctuations primarily provide information about the analyte’s size, shape, concentration, and net surface charge, respectively. One configuration for resistive pulse measurements is of an electrolyte-filled nanopipette immersed in a bath, where the nanopipette tip acts as the nanoscale orifice. It was previously shown that incorporating the large quantities polymer polyethylene glycol (PEG 8K, 50% w/v) into the external electrolyte bath dramatically enhanced …
Cotton Yield And Tissue-Potassium Response To Potassium Fertilization, Maria Paula Ramos Do Prado
Cotton Yield And Tissue-Potassium Response To Potassium Fertilization, Maria Paula Ramos Do Prado
Graduate Theses and Dissertations
Potassium (K) deficiency is a common yield-limiting factor in cotton (Gossypium hirsu tum L.) production across the U.S. Cotton Belt. Adequate fertilizer-K management is paramount to ensure optimum plant growth and development, minimizing yield losses. Currently, fertilizer K recommendations for cotton in Arkansas are derived from expected yield responses of other crops, with no calibrated fertilizer-K rates or critical tissue-K concentrations defined to maxim ize crop yield. Our research objectives were i) to investigate cotton yield response and fiber qual ity to K fertilization on soils with different K availability; ii) to correlate relative yield with tis sue-K concentrations at …
Measuring Agricultural Adaptation Using Remote Sensing: A Study Of Pigeonpea In Malawi, Maria Gorret Nabuwembo
Measuring Agricultural Adaptation Using Remote Sensing: A Study Of Pigeonpea In Malawi, Maria Gorret Nabuwembo
Graduate Theses and Dissertations
Drought, heatwaves, and flooding cause tremendous damage to agricultural production in Malawi, and the continuous production of maize has led to widespread soil degradation. Additionally, climate change is transforming the environment at a scale beyond historical records, posing significant challenges to social, political, and economic systems. To combat these issues, agricultural innovations have been implemented, such as the installation of irrigation systems and development of heat/drought resilient crop varieties. Pigeonpea is one of the prominent drought-tolerant and temperature resilient crops grown as a diversification strategy since it allows farmers to adapt to changes while maintaining subsistence needs. This research investigates …
Comparisons Between Traditional And Gluten-Free Cookies In Sensory Attributes, Acceptance, And Evoked Emotions, Eniola Ola
Graduate Theses and Dissertations
The rise in food allergies and intolerances, notably conditions like Celiac disease, as well as a general shift of consumers to a more health-conscious lifestyle has significantly increased the demand for gluten-free products. Consumers increasingly seek gluten-free products, yet these alternatives often face challenges in replicating the sensory qualities of traditional gluten-containing foods. This study explores the sensory attributes, emotional responses, and labeling impacts on consumer perception of gluten-free and gluten-containing cookies. The main objective of this thesis was to address the gap in research on sensory evaluation of gluten free foods, specifically in terms of commercially available products. The …
Identifying Microbial Complexities And Control Strategies At Pre-Harvest Poultry Production, Tolulope Tiwa Ogundipe
Identifying Microbial Complexities And Control Strategies At Pre-Harvest Poultry Production, Tolulope Tiwa Ogundipe
Graduate Theses and Dissertations
While significant progress has been made in post-harvest control of Salmonella in the poultry industry, the development of effective pre-harvest intervention strategies remains critical for reducing foodborne pathogens. Environmental reservoirs such as poultry litter and drinking water systems (DWS) are potential harborage sites for Salmonella and other microbial communities. Understanding the microbiota of these environments, including biofilms within water lines, is essential to identifying factors contributing to Salmonella colonization and improving pre-harvest control approaches. This study aimed to evaluate the incidence and quantity of Salmonella in poultry litter and water lines, characterize the microbial populations in these environments, and assess …
Examining The Combination Of Race-By-Age Segregation On Violence In Little Rock, Arkansas, Karissa Kruse
Examining The Combination Of Race-By-Age Segregation On Violence In Little Rock, Arkansas, Karissa Kruse
Graduate Theses and Dissertations
The Present study addresses the issue of residential segregation. Specifically, how the physical isolation of populations segregated based on both age and race affects violent crime. The sample includes all 155 Census block groups, which represent neighborhoods, in the city of Little Rock Arkansas. Data is accessed from the U.S. Census and the Little Rock Police Department. The current study asks: does the segregation of juveniles by age and race affect the distribution of crime? The theoretical frameworks guiding this research are social disorganization theory, structural-cultural theories of crime, the racialization of space perspective, and routine activities theory. The key …
Maria Sabina And The Wise Ones, Robin Bruce
Maria Sabina And The Wise Ones, Robin Bruce
Graduate Theses and Dissertations
Maria Sabina was born Maria Sabina Magdalena Garcia in 1894, in the principality of Huautla, located in Oaxaca, Mexico. She’s known as a healer and poet, though she would most likely refer to herself as a woman doctor, whose language becomes beautiful with the help of the psylocibin mushroom, which she affectionately refers to as little saints or holy children. In 1953, American banker, R. Gordon Wasson and his wife, pediatrician Valentina Pavlovna, were the first non-Indigenous people to witness one of Sabina’s ceremonies. Novice ethnomycologists and anthropologists, the couple traveled to Southern Mexico to witness a ceremony in which …
Variation In Stress Response Regulation Across Natural Isolates Of Saccharomyces Cerevisiae, Crystal Christine Crook
Variation In Stress Response Regulation Across Natural Isolates Of Saccharomyces Cerevisiae, Crystal Christine Crook
Graduate Theses and Dissertations
The stress response of Saccharomyces cerevisiae is a valuable area of study for understanding cellular adaptation mechanisms that govern survival in fluctuating environments. This dissertation investigates stress response regulation in yeast through the roles of Hog1 mitogen-activated protein kinase (MAPK) and the post-transcriptional regulator Mkt1, a key post-transcriptional regulator, in diverse wild yeast isolates. Hog1 is traditionally known for its central role in osmotic stress regulation in the high-osmolarity glycerol (HOG) pathway. This study expands the functional scope of Hog1 to include oxidative and ethanol-induced stress responses.
In parallel, we investigate post-transcriptional regulation of stress responses by the Mkt1 interactome, …
Impacts Of Toxic Fescue Seed Consumed By Pregnant Heifers On Maternal Blood Flow And Offspring Growth Parameters: Potential For Melatonin As A Therapeutic To Improve Offspring Growth, Rachel Scott
Graduate Theses and Dissertations
The southeastern United States is home to endophyte-infected (E+), toxic, tall fescue grass. Consumption of E+ fescue by livestock has been linked to fescue toxicosis, a disease reported to cost the United States beef industry around $1 billion in annual losses. The primary objective of this dissertation was to evaluate the influence of pregnant cattle consuming E+ seed during gestation on uterine artery blood flow and growth performance of female offspring. The secondary objective was to investigate melatonin supplementation as a mitigation strategy for expected fescue-associated decreases in uterine artery blood flow and offspring growth performance. The study reported in …
Crispr-Cas9 Integration Of Salmon Elovl2 In Zebrafish Enhances Lc-Pufa Biosynthesis And Growth Across Diets, Waleed Sami J. Al-Bachry
Crispr-Cas9 Integration Of Salmon Elovl2 In Zebrafish Enhances Lc-Pufa Biosynthesis And Growth Across Diets, Waleed Sami J. Al-Bachry
Graduate Theses and Dissertations
Fish are a rich source of EPA and DHA, which are well-known long-chain polyunsaturated fatty acids (LC-PUFAs). The sustainable production of these essential fatty acids poses significant challenges, mainly due to our dependence on marine fish and certain nuts. CRISPR-Cas system was utilized to insert the elongation of long-chain fatty acids-like2 (Elovl2) gene from Salmo salar into Danio rerio. Elovl2 was introduced at 10-15-minute post-fertilization, and successful integration confirmed by PCR genotyping, sequencing and expression of EGFP. Approximately 67% of injected embryos successfully exhibited EGFP and sequence results verified the gene integration of Salmo salar Elovl2 at the first start …
Fixed-Time Artificial Insemination Protocols Using Pre-Synchronization In Suckled Beef Cows, Wyatt Eugene Weber
Fixed-Time Artificial Insemination Protocols Using Pre-Synchronization In Suckled Beef Cows, Wyatt Eugene Weber
Graduate Theses and Dissertations
Adoption of artificial insemination by beef producers has been limited within the United States. Estrous synchronization (ES) for fixed-time artificial insemination (FTAI) has been one of the most effective ways to implement artificial insemination into beef production systems, but its efficiency has primarily been restrained by the dependency on the effectiveness of the initial gonadotropin releasing hormone (GnRH) injection in many FTAI protocols. The hypotheses of these studies were to determine practical methods of pre-synchronization to improve current FTAI protocols, ultimately increasing beef herd reproductive performance. The objective was to evaluate the use of prostaglandin F2α (PGF) and progesterone (P4) …
Penny For Your Thoughts: Exploring Mechanisms Linking Socioeconomic Status And Cognition, Colton Hunter
Penny For Your Thoughts: Exploring Mechanisms Linking Socioeconomic Status And Cognition, Colton Hunter
Graduate Theses and Dissertations
Socioeconomic status is a complex psychological construct linked to a number of professional and personal barriers, including cognitive deficits. Throughout this dissertation, I seek to examine the mechanisms that link socioeconomic status and cognition. First, I explore potential mediators in the link between adolescent family income and cognition in adulthood, identifying education, stressful life events, inflammation, and family education expectations as significant mediators. Next, I examine the effects of writing-based stress manipulations on long-term memory and working memory, as these effects may mimic stress from financial worry. In doing so, I identify potential effects on working memory parameters. Finally, in …
Bacterial Chondronecrosis With Osteomyelitis In Broiler Chickens: Experimental Lameness And Additive Probiotic Treatments, Amanda Anthney
Bacterial Chondronecrosis With Osteomyelitis In Broiler Chickens: Experimental Lameness And Additive Probiotic Treatments, Amanda Anthney
Graduate Theses and Dissertations
With the global population already surpassing 8.2 billion and expected to reach 9.7 billion by 2050, the poultry industry has seen a surge in demand due to its unmatched efficiency and affordability for consumers across the globe. Selective breeding and quality nutrition developments since the industry's commercialization have allowed producers to grow broilers up to 7 pounds by market age. While this extraordinarily efficient process has allowed quality poultry products to be available worldwide, the modern-day broiler has developed a multitude of musculoskeletal issues. Due to the lack of integral skeletal development to match the rapid production of muscle tissue, …
In Vitro And In Vivo Evaluation Of The Effect Of A Commercial-Dried Enterococcus Faecium Fermentation Product As Quorum Sensing Inhibitors Of Salmonella Enterica Typhimurium, Ileana Loeza
Graduate Theses and Dissertations
The purpose of the present research was to evaluate Enterococcus faecium fermentation peptides (EFP) for antibacterial activity, inhibition of biofilm formation, and anti-quorum sensing properties against Salmonella Typhimurium (ST) in in vitro trials and individual body weight and ST organ colonization in therapeutic and prophylactic models in broiler chickens. Ninety-six day-of-hatch Cobb 500 broiler chickens were randomized and assigned to one of four treatments: NC) Negative Control, no EFP treatment and no ST challenge; NC+EFP) Negative Control, EFP treatment and no ST; PC) Positive Control, no EFP treatment and ST challenge, and PC+EFP) EFP treated and ST challenged. Independent trials …
Conservation Agriculture Practice Effects On Greenhouse Gas Emissions From Fine-Textured Soils In Arkansas, Lauren Gwaltney
Conservation Agriculture Practice Effects On Greenhouse Gas Emissions From Fine-Textured Soils In Arkansas, Lauren Gwaltney
Graduate Theses and Dissertations
Biochar application and reduced tillage (RT) are both practices within the conservation agriculture framework, but specific impacts of these practices on direct greenhouse gas (GHG) emissions still require investigation in varied agricultural systems. This research aimed to evaluate the effects of biochar source (i.e., powder- and pellet-sized) and application rate (i.e., 0, 2.5, and 5 Mg ha-1) on GHG production in simulated furrow-irrigated rice (Oryza sativa) in a greenhouse experiment, and to evaluate the effects of RT relative to conventional tillage (CT) on GHG production in soybean (Glycine max) in southeast Arkansas. Both studies …
Developing Custom Fertilizer Strategies Recirculating Hydroponic Systems, Joshua Bryan Tebow
Developing Custom Fertilizer Strategies Recirculating Hydroponic Systems, Joshua Bryan Tebow
Graduate Theses and Dissertations
Hydroponic systems recirculate nutrient solution to reduce the amount of water used in leafy green production. However, the rootzone of the nutrient solution is complex and influenced by interacting factors such as plant uptake demand, irrigation water quality, supplied fertilizer salts and nutrient concentrations, environmental conditions, and injection of mineral acids/bases to control solution pH. A common strategy for managing nutrients in commercial production is to select a hydroponic solution formulation (i.e. recipe) to supply and maintain a target pH and electrical conductivity (EC) in the recirculating solution over time by automatic injection of mineral acids/bases and concentrated fertilizer stock …
Implications Of Acetylation And Phosphorylation On Metabolic Enzymes, Nour Fatema
Implications Of Acetylation And Phosphorylation On Metabolic Enzymes, Nour Fatema
Graduate Theses and Dissertations
All living things depend on glycolysis and the TCA cycle for energy production and metabolic control. Post-translational modifications (PTMs) such as acetylation and phosphorylation, which can change enzyme activity, substrate binding, and overall metabolic balance, tightly regulate these pathways. Diseases like cancer and metabolic problems are associated with disruptions in these alterations.
Serine phosphorylation and lysine acetylation are two of the many PTMs that are evolutionarily conserved processes that alter protein function. Previously thought to be a static histone alteration, acetylation is now understood to be a dynamic regulator of metabolic enzymes that modifies contacts, charge, and conformation. In response …
Exploring The Role Of Influencer Marketing In The Animal Health Industry: A Content Analysis Of Strategies And Impact, Haylee Henry
Exploring The Role Of Influencer Marketing In The Animal Health Industry: A Content Analysis Of Strategies And Impact, Haylee Henry
Graduate Theses and Dissertations
A review of literature revealed a lack of peer-reviewed research on influencer marketing within the animal health and nutrition industry, specifically within the livestock sector. This study aimed to address this gap by analyzing how animal health and nutrition companies of different sizes utilized influencer marketing and how consumers interacted with content tailored to influencer marketing. A content analysis was conducted. This study collected and analyzed social media influencer posts from eight North American animal health and nutrition companies, three small, three medium, and two large, between January 2023 and December 2024. Influencer content included posts by individuals, partner organizations, …
The Evolving Changes In The Regionalization Of Export Bans On U.S. Poultry Trade From The 2015 And 2022 Highly Pathogenic Avian Influenza Events, Kylie A. Roesler
The Evolving Changes In The Regionalization Of Export Bans On U.S. Poultry Trade From The 2015 And 2022 Highly Pathogenic Avian Influenza Events, Kylie A. Roesler
Graduate Theses and Dissertations
As food security becomes a growing concern worldwide in a trading system dominated by globalization, the safeguarding of a bio-secure food system is at risk. One of the greatest risks to this stability of the system is, highly pathogenic avian influenza or HPAI which is growing in frequency and impact. Therefore, the global response to disease events is evolving into a newfound understanding of disease prevalence across the animal protein industries. This study will analyze the 2022 HPAI event in the United States regarding the regionalization of restrictions imposed by trading partners accounting for trade agreements executed. The examination of …
Physiological And Molecular Responses Of Diverse Rice Genotypes Under Drought Stress, Huda Mohmmed Alshaya
Physiological And Molecular Responses Of Diverse Rice Genotypes Under Drought Stress, Huda Mohmmed Alshaya
Graduate Theses and Dissertations
Climate change-induced drought stress is a significant constraint on global rice (Oryza sativa L.) production, threatening food security. This study evaluated the drought resilience of 15 diverse rice genotypes from the USDA mini-core collection under field, greenhouse, and osmotic stress conditions. Field trials assessed reproductive-stage drought tolerance based on panicle length (PL), number of spikelets per panicle (NSP), and spikelet sterility (SS). Greenhouse experiments examined moisture retention at the vegetative stage. Significant genotypic variation was observed, with genotypes 310724, 310779, 311181, 311603, 311793, and Vandana exhibiting drought tolerance through stable PL and SS. Additionally, genotypes 310100, 310428, 311255, N22, …
Polyphenol-Protein Interactions In Pigmented Cereals – A Clean-Label Approach To Tailor Starch Viscosity, Annegret Jannasch
Polyphenol-Protein Interactions In Pigmented Cereals – A Clean-Label Approach To Tailor Starch Viscosity, Annegret Jannasch
Graduate Theses and Dissertations
Viscosity plays an important role in the food industry as it affects the quality, processing stability, and shelf-life of food and impacts physiological responses such as satiation and satiety. Greater viscosity reduces food intake by increasing the perception of satiety, primarily by delaying gastric emptying and increasing tactile stimulation. Starch is commonly added to food products as a viscosifying ingredient, which often involves chemically modifying the starch via cross-linking, to enhance its resistance to high shear, temperature and acidity. However, chemically modified starches are not “clean-label” ingredients due to the use of toxic chemicals. Therefore, further exploration is needed to …
Quick Response Codes In Extension Outreach Communications: Insights From A Visual Content Analysis And Educators’ Perceptions, Kobina Danful Fanyinkah
Quick Response Codes In Extension Outreach Communications: Insights From A Visual Content Analysis And Educators’ Perceptions, Kobina Danful Fanyinkah
Graduate Theses and Dissertations
Increased smartphone use presents an opportunity to enhance the creative inclusion of Quick Response (QR) codes in extension outreach efforts. Tailoring extension programs to communicate with specific audiences has been identified as essential, highlighting the need for targeted and context-specific strategies. The development of QR code technology has provided a unique approach that allows extension professionals to target clientele with specific extension messages and programs. While extension has a commendable record in educational delivery through traditional forms of outreach, research on how best to employ digital tools with extension audiences is thin at best. Though the rising popularity of QR …
An Analysis Of Cellular And Tissue Function Of Lipin In Drosophila Melanogaster, Judah Christian Scott
An Analysis Of Cellular And Tissue Function Of Lipin In Drosophila Melanogaster, Judah Christian Scott
Graduate Theses and Dissertations
Regulation of energy expenditure and energy storage is a key characteristic of life. Numerous enzymes play a crucial role in regulating energy metabolism. Lipins are a highly conserved family of proteins that function in both lipid synthesis and gene regulation. In the glycerol-3 phosphate pathway, lipins act as a phosphatidate phosphatase (PAP) producing diacylglycerol. In gene regulation, lipins act as transcriptional co-regulators of metabolic genes. The enzymatic PAP activity occurs in the cytoplasm; however, for lipins to function as gene regulators, they must translocate into the nucleus. Nuclear translocation is facilitated by the nuclear localization signal (NLS) within the protein. …
Bacterial Chondronecrosis With Osteomyelitis In Broilers: Etiology And Selected Mitigation Measures, Khawla Saud Alharbi
Bacterial Chondronecrosis With Osteomyelitis In Broilers: Etiology And Selected Mitigation Measures, Khawla Saud Alharbi
Graduate Theses and Dissertations
Bacterial chondronecrosis with osteomyelitis (BCO) is the predominant cause of lameness in broiler chickens. It mainly impacts broiler production and animal welfare, causing substantial financial losses accounting for 1- 2% due to bird condemnations at the marketing age. Remediating BCO lameness disease demands investigating its causes using appropriate models and evaluating preventive measures, such as enhancing epithelial tissue health and tight junction integrity in the digestive and respiratory tract, strengthening immune functions, and improving bone strength. Therefore, the objectives of this dissertation were to 1) study predisposing factors for BCO lameness by investigating the effects of deoxynivalenol (DON) and fumonisin …
#Girls Gone Viral: Girlification Trends And The Digital Postfeminist Sensibility, Madison Rose Mcbride
#Girls Gone Viral: Girlification Trends And The Digital Postfeminist Sensibility, Madison Rose Mcbride
Graduate Theses and Dissertations
This thesis provides a critical analysis of viral girlification trends on TikTok to bolster understanding of the rhetoric of popular feminism in the emerging digital landscape. Grounded in feminist cultural theory, this thesis posits that women’s digital engagement influences their notions of self-actualization, their avenues for resisting sexist ideals, and ultimately, their political mobilization. Extending scholarship on postfeminism, I offer two unique but co-constructive features of the postfeminist sensibility: post-irony and the confession paradigm. The first chapter analyzes the “girl dinner” trend as a site of post-ironic discourse. Characterized by ambiguity and ambivalence, post-ironic discourse muddles the line between sincerity …