Digital Commons Network^™

Recombinant Production And Purification Of Green Fluorescent Protein (Gfp)-Fused Metal Binding Protein For Palladium Nanoparticle Synthesis, Shadrach Ibinola

Graduate Theses and Dissertations

In lieu of chemical and physical methods, biologically guided synthesis is increasingly used as a cost-effective medium for the fabrication of nanoparticles (NP). Recently, a palladium metal binding sequence Pd4 (TSNAVHPTLRHL) has been demonstrated to be instrumental in the production of palladium (Pd) nanoparticles. Although, by eliminating the additional cost of purification of the protein, the crude lysate of E. coli containing Pd specific protein has been proven to be a viable cost-effective means for the synthesis of Pd NP, studies have not be done to ascertain the comparative catalytic activity of nanoparticles synthesized from both clarified lysate and pure …

Enzymatic Degradation Of Microcystin-Lr By Microcystinase (Mlra), Faisal Alqhtani

Graduate Theses and Dissertations

Microcystin-LR (MC-LR) is affecting the water supply worldwide. Hence, a way to eliminate this toxin is an essential target. In this study, successful cloning of the mlrA gene and producing MlrA enzyme that can degrade the cyclic MC-LR to linearized MC-LR was done. MlrA protein was expressed in Escherichia coli BL-21 (E. coli). Also, enhancing the MlrA yield by adding nickel to LB media was a success in producing more MlrA enzyme from the same volume. Even though the enzyme showed no activity after adding Ni, the enzyme was expressed at a higher yield. Furthermore, it was to investigate adding …

Badnaam Women Of Bollywood: The Combative Sexual Politics Of Item Songs In India, Ketaki Deshpande

Graduate Theses and Dissertations

This study analyzes the controversial Bollywood convention “item number,” a vampy song with suggestive lyrics and hypersexualized imagery of dancing women, as a crucial cultural artefact reflecting the gender and sexual uneasiness in India. Sex avoidance is a common theme in Bollywood films and in the country, where media and people are heavily censored and policed by anti-Romeo squads under the Modi administration. The item girl breaks the sexual tension with a mega hit song and hook step that fuels the economy of dance and desire in India. While the song is completely unrelated to the narrative of the film, …

Comparing Organic And Conventional Yield Responses To Climate Variations, Joost Wilken Meyer

Graduate Theses and Dissertations

This thesis compares the responses of organic yields and conventional yields towards climate variations. To achieve this objective, weather variables such as growing season weather conditions (average temperature, precipitation, cloud cover, relative humidity, drought index), weather anomalies, the occurrence of severe or extreme droughts and excessive rainfalls, are combined with 23 data sets gathered from previous studies that compare organic and conventional yields from the same location and time periods. To narrow the scope, the thesis focuses on soybean, maize, and wheat production in Europe and North America. Study-level fixed-effects models are used to control for any time-invariant factors such …

Dissonance Between Christian Beliefs And Eating Habits In The South, Karli Dianne Stringer

Graduate Theses and Dissertations

The purpose of this qualitative study was to initiate understanding of how obesity in the South is still so prevalent even though the majority of inhabitants subscribe to a faith that discourages unhealthy lifestyles. Furthermore, the information presented in this research sought to fill the knowledge gap for communicators and educators concerning the dissonance between Christianity in the South and the unhealthy eating habits of Southerners. Grounded in the Cognitive Dissonance Theory, this study comprised of a semi-structured interview route in which Protestant evangelical Christians in the South (N = 11) participated in a descriptive study conducted by a committee …

The Impact Of Corporal Punishment For Timorese High School Graduates, Veronica Godinho Pereira

Graduate Theses and Dissertations

This research examines the impacts of corporal punishment for Timorese high school graduates. Physical punishment is a pervasive method of disciplining students and children used in Timor-Leste because it has been such a tradition (UNICEF 2017). Few researchers have attempted to analyze the negative impacts of corporal punishment and possible gender differences; there is no known research on the impacts of corporal punishment in Timor-Leste. This study uses an in-depth interview method, where data were collected from 26 Timorese high school graduates composed of both men and women from both private and public schools in Timor-Leste. The ages of the …

The Morphodynamic Interaction Of River Deltas And Their Marshes, Kelly M. Sanks

Graduate Theses and Dissertations

Globally, many of the largest river deltas contain vast marsh platforms that are currently threatened due to a combination of anthropogenic alterations to rivers and increasing relative sea level rise. Restoration and management plans for river deltas depend on optimizing riverine sediment accumulation in marsh platforms. However, the accumulation of organic material in marsh platforms is often neglected in predictive models and the interaction of ecogeomorphic processes governing marsh accumulation with the physical processes governing river delta growth is poorly understood.

Herein, I investigate this complex relationship through a combination of field and experimental studies. I show that in coastal …

Yumiko Ōshima’S The Star Of Cottonland: A Translation, Sheena Woods

Graduate Theses and Dissertations

This is a translation of Volume 1 of Yumiko Ōshima’s The Star of Cottonland.

The Nature Of Informal Educators In Science Centers, Science Museums And Zoos: Case Studies Of Personnel, Practices, Programs, Outreach And Organization, Courtney Erickson

Graduate Theses and Dissertations

Informal Science Centers provide educational experiences for people across the country daily. While the terminology is often similar, there are differences between institutions, often determined by mission, organizational structure, and experience. The purpose of this study was to identify shared experiences and unique characteristics of eight informal science centers around the country. The significance of the study was to add to the understanding of informal science educators' responsibilities, organizational structure, decision-making process, and development. Data for the study was collected using qualitative measures through a researcher-created interview, sent to fifteen institutions, after the recommendation from two well-known informal science researchers. …

Queering The Metanarrative Of Domesticity: Chosen Families In Late Nineteenth- Century American Women's Literature, Sharla Rosenbaum

Graduate Theses and Dissertations

My primary purpose in this project is to dispel the notion that the white, bourgeois, patriarchal, nuclear family believed by millions of US citizens to have once monopolized the United States’s cultural landscape through an examination of how women authors in the United States constructed families in literature immediately following the Civil War. Not only do the novels under examination reflect diverse representations of families, but they also reflect those images from diverse perspectives. I will use a Marxist lens to explore class and ideologic interpellation; a renegade global feminist lens to explore failure and differential consciousness; a queer and …

International Students In The Campus Carry Debate: A Descriptive Phenomenological Inquiry, Andre Richardo Grant

Graduate Theses and Dissertations

This qualitative phenomenological study purposefully sampled 15 graduate international students at a Southern University (SU). Semi-structured interviews were used to determine their perceptions and attitudes regarding the implementation of campus carry policies at the institution. The study aimed to answer the following research questions: How has the implementation of the campus carry policy at SU impacted international students’ perceptions of their experiences at the institution? How has the implementation of campus carry policies at SU influenced international students’ perceptions of the social ecology of the institution? and How did SU formally include international students in the on-campus campus carry policy …

Listening To Voices Of Latinx Immigrants In Rural America, Jacqueline Smith

Graduate Theses and Dissertations

The purpose of this phenomenological qualitative study was to capture and gain an understanding of Latinx immigrants’ lived experiences during their transitions from their native countries to rural Arkansas. Using purposeful criterion-based sampling, the population of interest was five Latinx immigrant students and families who migrated from their native countries to the state of Arkansas. The analytic strategy included a single-case model of each family’s transcription, two-case model, cluster analysis to identify likeness, document portrait, and subtheme development. The structural descriptions that formed the essence of the experience resulted in three themes: education, migration, and emotional experiences. The three overall …

Longitudinal Monitoring Of Tumor Response To Immune Checkpoint Inhibitors Using Diffuse Optical Spectroscopy, Joel Isaac Rodriguez Troncoso

Graduate Theses and Dissertations

Immune checkpoint drugs have completely changed the way people treat metastatic melanoma and non-small-cell lung cancer. While the impacts of these immunological checkpoints and their suppression on T cell function are well characterized, their consequences on the tumor microenvironment are not. In a CT26 mouse colorectal cancer model, we employed diffuse reflectance spectroscopy to track in vivo tumor microenvironmental alterations in response to immune checkpoint inhibitors. On three separate days, animals bearing CT26 tumor xenografts were given anti-PD-L1, anti-CTLA-4, a combination of both inhibitors, and isotype control. Within the first 6 days, monotherapy with either anti-PD-L1 or anti-CTLA-4 resulted in …

Development And Evaluation Of Models For Hatchery-Mediated Infection Of Neonatal Broiler Chicks, Brittany Danielle Graham

Graduate Theses and Dissertations

Formaldehyde fumigation has been used to control the microbial load in commercial hatch cabinets. The hatch cabinet environment promotes replication and dissemination of both apathogenic and pathogenic microorganisms. As the microbial load increases during the hatching phase, formaldehyde eliminates airborne microorganisms circulating in the hatch cabinet environment. Due to the hazardous properties of formaldehyde, non-toxic alternatives to formaldehyde fumigation to control the microbial bloom in hatch cabinets are needed. The objectives of the present dissertation were to develop challenge models using singular or multiple microorganisms associated with the microbial bloom to simulate contamination that occurs in commercial hatch cabinets. Initially, …

Evaluation Of Two Neonatal Challenge Models For Broiler Chickens And Their Effects On Early Performance Parameters, Callie Selby

Graduate Theses and Dissertations

Formaldehyde fumigation in poultry hatch cabinets has been utilized for sanitation of hatching eggs for over a century. Formaldehyde is utilized to minimize pathogenic microbes on the surface of the egg as well as the microbial bloom during the hatching process. While formaldehyde is effective, its use is regulated in the United States and Europe due to its carcinogenic nature. Formaldehyde has been shown to damage the cuticle of the egg, cause embryonic death, and damage the epithelial lining of the respiratory tract of freshly hatched chicks, predisposing them for respiratory infection. Alternatives for formaldehyde fumigation must be identified and …

Just Benjamin, Paige R. Murphy

Graduate Theses and Dissertations

This documentary short film is about a now 30-year-old transgender man telling his story of growing up queer in a small Arkansas town, from being outed as a lesbian in high school, to ultimately being accepted as a trans man by members of his military and biological families. Benjamin “Benji” Davis grew up in the small town of Friendship, Arkansas, enlisted in the U.S. Army shortly after high school and served for six years, then came back to Arkansas and primarily lived in Little Rock until he moved to Denver, Colorado this fall. The documentary is structured linearly both with …

Symbolism Or Substance: Factors Influencing Campus Messaging Decisions And Their Alignment With Institutional Values, Laura Herzog Jacobs

Graduate Theses and Dissertations

Historically, institutions of higher education (IHE) have been cautious and slow in making changes. Yet in today’s fast-paced and dynamic world of content availability and information flow generated by 24-hour news cycles and the ubiquity of social media present a challenging reality for colleges and universities in their public relations and communications approaches (Sands & Smith, 2000). Creating a harmonious and agile approach to managing these responsibilities against the backdrop of an incessant flow and public demand for information, including responses to national events often with seemingly little or no connection to the institution, suggested a need to examine the …

Investigation Of Histomoniasis Prevention In Poultry, Lesleigh Beer

Graduate Theses and Dissertations

Histomonas meleagridis is the etiological agent of histomoniasis, also commonly known as blackhead disease. This protozoal disease of poultry is detrimental to turkeys with flock mortalities often reaching 80-100%, although other gallinaceous birds are susceptible. Since the voluntary removal of nitarsone in 2015, the poultry industry is suffering with no approved prophylactics, therapeutics, or vaccines for this disease. The objectives of this dissertation were to evaluate multiple methods for prevention or control of histomoniasis, including dietary chemoprophylaxis and vaccination. Specifically, this research evaluated quinine as a chemoprophylactic candidate (Chapter 3) or live-attenuated H. meleagridis as vaccine candidates (Chapter 4) in …

Site-Specific Effects Of Lysine Acetylation On Aminoacyl-Trna Synthetase, Hao Chen

Graduate Theses and Dissertations

Aminoacyl-tRNA synthetases (AARSs) are an ancient and highly conserved family of enzymes which can catalyze a two-steps aminoacylation reaction to charge tRNAs with their cognate amino acids, thus playing crucial roles in ribosomal protein synthesis. Naturally, the accurate amino acids and tRNA recognition of these synthetases are essential to the fidelity of translation process. To assure the correct recognition, some of these synthetases have evolved with an editing function to help remove the mischarged tRNAs. In addition to these functions, AARSs are also involved in various biological processes ranging from transcription to translation. Currently, a series of proteomic studies have …

Stress Among Ncaa Division Ii Head Coaches, Dee Gerlach

Graduate Theses and Dissertations

The purpose of this quantitative study was to identify factors that create stress among head coaches at the National Association Athletic Association Division II level. Data was collected through a demographic questionnaire and the Coaching Issues Survey (CIS), a tool used to measure specific factors that may create stress among coaches. The factors of the CIS include four subscales: Athlete-Concerns, Time-Role, Program-Success, and Win-Loss. Participants (N=416) consisted of head coaches representing the following sports: baseball, men’s basketball, women’s basketball, men’s golf, women’s golf, men’s and women’s golf, softball, women’s tennis, and men’s and women’s tennis. The independent variables for this …

Efficacy Of Selected Powdered Floor Treatments And Turmeric Rhizome Powder Against Salmonella, E. Coli, And L. Monocytogenes On Polyurethane-Concrete Flooring Material Carriers, Robert Sidney Fuller

Graduate Theses and Dissertations

The flooring in a food processing environment can become contaminated with pathogenic bacteria in many ways including foot and equipment traffic, incoming materials, and drain backups. Turmeric (Curcuma longa L.) rhizome powder and seventeen other commercially available powdered floor treatments with a variety of active ingredients including quaternary ammonium compounds (QAC), sodium percarbonate and similar perhydrates (SPC), urea, and borax, were investigated for reducing the levels of pathogens on flooring thereby reducing the risk of cross-contamination from the floor to food contact surfaces. Some of the commercially available floor treatments were Environmental Protection Agency (EPA) registered antimicrobials while others were …

Investigation Of Iron-Nickel Films Based Catalysts: Controlling Hydrogen Evolution Reaction (Her) Through An Electrode-Electrolyte Engineering Approach, Sergio Ivan Perez Bakovic

Graduate Theses and Dissertations

Electrochemical energy conversion technologies need to be economically viable in order to be adopted. Earth-abundant transition metals (i.e., Fe, Co, and Ni) have attracted attention due to their high availability, low cost, and catalytic characteristics that are in many cases found in and exploited by nature. Herein, we synthesize FexNi100-xOy electrocatalyst films and study how the electrocatalyst-electrolyte interactions alter the hydrogen evolution reaction (HER).In this work, FexNi100-xOy films were synthesized to study the composition, chemical state, and morphology. Characterization results provided a well-established electrocatalyst film platform to then proceed with electrochemical evaluations. The effects of the electrode composition and electrochemically …

How Are Agricultural Research Projects Conceiving Innovation? An Assessment Of The European Union Multi-Actor Projects, Samuel Brea Martinez-Collado

Graduate Theses and Dissertations

Horizon research programs are the European Union’s lighthouse for innovation and research concerning the agri-food chain, the management of natural resources, and the bioeconomy. Their innovation strategy approach is one that pursues the practical application of the research via the collaboration of all actors involved. Such strategy is the reflection of a Multi-Actor Approach as their procedure to enhance innovation. This paper presents the main trends and directions 101 Multi-Actor Projects (MAPs) are following on said approach towards innovation. The analysis is based on the use of well-defined “pathways” and “measures” proposed in the Guidelines for Evaluation of Innovation in …

Designing Digital Antiquity: Classical Archaeology In New Virtual Applications, William Loder

Graduate Theses and Dissertations

In this thesis, I argue that the combination of existing archaeological theory with game design theory offers an innovative avenue for creating serious 3D applications of archaeological sites in virtual reality that can be productively used for pedagogical, research, and outreach solutions. In this thesis, I engage with the archaeological theories of phenomenology and sensory studies, briefly touching on structure and agency as well as discussion of some current digital applications in use in the field. For this project, I am interested in game design theory as it relates to education and I view Virtual Reality as an important tool …

Statistical Modeling, Learning And Computing For Stochastic Dynamics Of Complex Systems, Mohammadmahdi Hajiha

Graduate Theses and Dissertations

With the recent advances in sensor technology, it is much easier to collect and store streams of system operational and environmental (SOE) data. These data can be used as input to model the underlying behavior of complex engineered systems and phenomenons if appropriate algorithms with well-defined assumptions are developed. This dissertation is comprised of the research work to show the applicability of SOE data when fed into proposed tailored algorithms. The first purposes of these algorithms are to estimate and analyze the reliability of a system as elaborated in Chapter 2. This chapter provides the derivation of closed-form expressions that …

Effects Of Localized Oxygen Production By Electrolysis On The First-Generation Glucose Sensor Response, Nandita Halder

Graduate Theses and Dissertations

Glucose sensors are very important for detecting blood glucose both in vitro and in vivo. First-generation glucose biosensors were based on the glucose oxidase (GOx) enzyme using molecular oxygen as the electron acceptor and therefore oxygen dependent. Unfortunately for in-vivo work, oxygen in the body is variable and limited. Alternative approaches to overcome the oxygen dependency came with their own limitations. The widely used and commercially available ex-vivo glucose test strip uses a mediator in place of oxygen to free it from oxygen dependency. The mediator-based technology, in most cases cannot be transferred to in vivo applications due to the …

Sigesn Light-Emitting Devices: From Optical To Electrical Injection, Yiyin Zhou

Graduate Theses and Dissertations

Si photonics is a fast-developing technology that impacts many applications such as data centers, 5G, Lidar, and biological/chemical sensing. One of the merits of Si photonics is to integrate electronic and photonic components on a single chip to form a complex functional system that features compact, low-cost, high-performance, and reliability. Among all building blocks, the monolithic integration of lasers on Si encountered substantial challenges. Si and Ge, conventional epitaxial material on Si, are incompetent for light emission due to the indirect bandgap. The current solution compromises the hybrid integration of III-V lasers, which requires growing on separate smaller size substrates …

Systematics Of Eastern North American Podothrombium (Parasitengona: Podothrombiidae), Kelsey Lynn Cline

Graduate Theses and Dissertations

Velvet mites of the genus Podothrombium Berlese, 1910 are found worldwide with only two described species known from North America, both occurring on the west coast. The present study describes five new species occurring in the east, from both larval and post-larval stages. Species hypotheses are supported with morphology and analysis of mitochondrial (barcoding region of COI) and nuclear genes (D2-3 expansion regions of 28S rDNA). Specimens are incorporated from the Barcode of Life Data Systems (BOLD), allowing our dataset to span most of North America. Therefore, we were able to raise the number of Podothrombium in North America from …

Age, Ty Barnes

Graduate Theses and Dissertations

Age is a body of work that uses open ended, multi-directional narrative, economical craft, body positioning, and disorder to create situations for curiosity to take hold and rekindle a sense of naivety. Intentionally pedestrian material choice and playful, curious methodology work in tandem with the visual language of play to create a world building opportunity for the participating viewer. The objects are anchors or starting points with spaces in between for flexible narratives and imagined and reimagined worlds with no prescribed beginning or end point.

The exhibition and written thesis represent a conglomeration of connected-by-association ideas, a Rube Goldberg machine …

The Systemic Punches: Displacement Experience Of Vulnerable Immigrants, Eric Andre

Graduate Theses and Dissertations

"The Systemic Punches: Displacement Experience of Vulnerable Immigrants" focuses on the impact of systems of state control such as immigration laws, policies, and practices that have been institutionalized and that have marginalized immigrants. In my thesis, I pay specific attention to inhuman acts of exclusion and discrimination resulting from the systemic barriers perpetuated by xenophobic and nationalist ideologies.

From this standpoint, my thesis exhibition employs interactive space, which includes visual art (drawing, sculpture ceramics), projection, video, and sound, as a means to explore the effects of the exclusive and discriminatory immigration policies and practices. Furthermore, it is designed to explore …