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The Expression Of Recombinant Cas9 Proteins In E. Coli Bl21(De3) Strains, Morgan Reese
The Expression Of Recombinant Cas9 Proteins In E. Coli Bl21(De3) Strains, Morgan Reese
Graduate Theses and Dissertations
Abstract Extensively used for genome editing, CRISPR-Cas9 is a relatively new tool that has made a major impact in the genomic engineering landscape. Due to this, the production of CRISPR proteins that are not commercially available can limit research applications. Here, we present methods for optimizing laboratory scale production of CRISPR proteins including Cas9. The method uses IPTG to induce gene expression of the specified Cas9 and nickel column purification to isolate proteins. CRISPR proteins are validated for purity by SDS-PAGE gel, western blot, and for activity by in vitro digestion. We thoroughly validate and compare the effectiveness of BL21(DE3) …
Advancing Qualitative Inquiry In Agricultural Communications: An Analysis Of Theories, Methodologies, And Standards For Quality, Karli Yarber
Graduate Theses and Dissertations
This dissertation offers a deep dive into the evolving landscape of qualitative research within the field of agricultural communications. Through a trio of research articles, this research critically assesses the application of theories, methodologies, and standards for quality across studies published in six disciplinary journals from 2003 to 2023. The first manuscript explores the theoretical frameworks guiding qualitative research in agricultural communications, uncovering a diverse but scattered theoretical underpinning with Framing, Diffusion of Innovations, and Uses and Gratifications Theory emerging as the most prevalent. The second manuscript meticulously examines the methodologies employed, revealing a growing acceptance and application of qualitative …
Perceptions Of School-Based Agricultural Education Teachers Toward Promoting Agricultural Education Teaching As A Career, Emmanuel Kwadwo Sekyere
Perceptions Of School-Based Agricultural Education Teachers Toward Promoting Agricultural Education Teaching As A Career, Emmanuel Kwadwo Sekyere
Graduate Theses and Dissertations
School-based agricultural education has experienced a scarcity of qualified teachers over the years. To reduce the prevalence of school-based agricultural teacher shortages, research has led to the adoption of several approaches in schools across the country. The purpose of this study was to examine the perceptions of school-based agricultural education teachers toward promoting agricultural teaching as a career. The theoretical framework for this research was based on the Theory of Planned Behavior. The research objectives were to determine the perceived importance of career promotion activities, the frequency of career promotion activities, the perceived job satisfaction of SBAE teachers, and the …
Describing Benefits, Challenges, And Barriers Of Offering Adolescent Programs At Public Gardens In The United States, Hiliary Rodgers
Describing Benefits, Challenges, And Barriers Of Offering Adolescent Programs At Public Gardens In The United States, Hiliary Rodgers
Graduate Theses and Dissertations
Research shows a lack of educational programs for the adolescent audience at public gardens in the United States. Along with benefits that adolescent programs create for the institutions that offer them, there are also challenges associated with designing programs for this specific audience, and barriers preventing public gardens from offering them. There is little research related to adolescent programs at public gardens in the United States, aside from a 2010 study by Purcell et al. that explored the benefits, challenges, and barriers of adolescent programs. The current study aimed to determine the prevalence of adolescent programs at public gardens in …
Constructing Trust Among Large-Scale Organizations: An Archaeological Case Of Collective Action From Tlaxcallan, Mexico, Marc Daniel Marino
Constructing Trust Among Large-Scale Organizations: An Archaeological Case Of Collective Action From Tlaxcallan, Mexico, Marc Daniel Marino
Graduate Theses and Dissertations
Within nation-states, agencies, cooperatives, and other groups seeking to coordinate in the management of resources, conflicting interests can hinder cooperation and preclude joint action. Increasing transparency in resource management policies and facilitating communication among groups can reduce cooperative dilemmas by building trust. Developments in collective action research have demonstrated that increased communication and transparency among participants can build trust because future outcomes and behaviors can be predicted based on observations of past actions. Archaeology provides case studies to explore some of the earliest attempts by nation-states to reduce tensions caused by competing factions. This dissertation explores one such case study …
Turfgrass Soil Surfactants: Examining Active Ingredients, Application Strategies, And The Structure–Function Relationship In Sand-Based Putting Greens, Daniel O'Brien
Graduate Theses and Dissertations
Soil surfactants, commonly referred to as wetting agents, are a leading water management strategy for golf courses. Non-ionic block copolymers are the most common type of turfgrass soil surfactant. However, differences in the registration process and labeling requirements allow soil surfactants to enter the marketplace accompanied by far-less data compared to other products used on golf courses. This has led to considerable confusion among golf course superintendents. Three primary sources of confusion are: (i) the widely-used marketing terminology of “penetrants” and “retainers” to classify wetting agents; (ii) active ingredients which in some cases are not disclosed at all; and (iii) …
Examining The Relationship Between Mobile Phone Utilization On Self-Directed Online Professional Development Among Early Childhood Practitioners: A Preregistered Study, Jennifer Bowman
Graduate Theses and Dissertations
This study examines Arkansas early childhood practitioners' propensity for self-directed learning when using mobile phones to participate in self-paced online professional development. Further, the activity system model provides context for understanding practitioners' willingness to use technology, like smartphones, to adopt new information when interacting in online learning formats. The problem is that although we must offer professional development to ensure we have competent early childhood practitioners statewide, we are uncertain whether those practitioners have the propensity to use mobile phones to direct their online learning. The purpose of this cross-sectional regression study is to examine the relationship between early childhood …
High Performance Silicon Carbide Based Converters For Powertrain Electrification, Nan Lin
High Performance Silicon Carbide Based Converters For Powertrain Electrification, Nan Lin
Graduate Theses and Dissertations
With the increasing concern towards environment protection, electric vehicles (EVs) and hybrid electric vehicles (HEVs) have become popular in recent years. They not only help reduce carbon emissions, but also come with other benefits such higher efficiency and better user experience. Many countries and vehicle manufactures have made their plans to keep up with this trend in vehicle electrification. Powertrain is an assembly of components in a vehicle that pushes it forward. As for EVs and HEVs, their powertrains are composed of one or more power electronics converters, which are significant to the system. Silicon carbide MOSFETs have shown superior …
Determination Of Factors Influencing Microbial Food Safety Risks Of Additive Manufacturing And 3d Printing Of Food, Allyson Nichole Hamilton
Determination Of Factors Influencing Microbial Food Safety Risks Of Additive Manufacturing And 3d Printing Of Food, Allyson Nichole Hamilton
Graduate Theses and Dissertations
The burgeoning field of 3D food printing introduces opportunities for customizing the physicochemical properties of food, offering tailored solutions in color, shape, and texture. Despite its potential, the implications of this technology on food safety, particularly the efficacy of manufacturer-recommended cleaning protocols for stainless steel food ink capsules, the transferability of pathogens from a contaminated food ink capsule into the food ink based on macronutrient composition of the food, and the impact of storage conditions on pathogen viability, have not been thoroughly investigated. This dissertation provides a comprehensive analysis of these aspects, focusing on the control of foodborne pathogens and …
Risk, Protection And Mental Health Among Incarcerated People, Cecilie Froulund Jensen
Risk, Protection And Mental Health Among Incarcerated People, Cecilie Froulund Jensen
Graduate Theses and Dissertations
Approximately 40 percent of incarcerated adults experience some history of mental illness, whereas prevalence in the general population is less than 20 percent (Criss & John, 2023). The Survey of Prison Inmates (SPI) is a self-reported survey, examining numerous aspects of prisoners’ life. It has been used to quantify the prevalence of both physical and mental health problems, however, little or no examination regarding the underlying health determinants has been done. The current study assessed state prisoners in the United States and examined both risk and protective factors (historical and current) and their relationships to mental health. The results showed …
Key Practices In P-6 Integrated Stem Education: A Delphi Panel Investigation, Leah R. Cheek
Key Practices In P-6 Integrated Stem Education: A Delphi Panel Investigation, Leah R. Cheek
Graduate Theses and Dissertations
Throughout the past two decades policymakers, practitioners and researchers have been summoned to pursue best practices to address and implement improvements in STEM education for P-12 students (Archer, Moote, MacLeod, Francis, & DeWitt, 2020; Emrey-Arras, 2018; Hodson, 2003; National Research Council, 2012). Irrespective of the longstanding investment of time and resources towards STEM education, students’ patterns of STEM involvement in schools continue to be persistently unchanged (Archer, et al., 2020; Ro & Knight, 2016) and even fewer Americans are entering STEM disciplines in the workforce (National Science Foundation, 2018). The purpose of this three-article dissertation study is to support effective …
Salafi Reformist Fatwas And The Challenge To Traditionalism In Egypt And Arabia, 1895 – 1930, Samuel Scurry
Salafi Reformist Fatwas And The Challenge To Traditionalism In Egypt And Arabia, 1895 – 1930, Samuel Scurry
Graduate Theses and Dissertations
Studies of the Islamic intellectual movement commonly known as Salafism have long focused either on the modernist reformers in Egypt and Syria in the late nineteenth and early twentieth centuries, or on the far more austere and sometimes militant iterations of Salafism from the mid-twentieth century to the present day, often associated with Wahhabism. A handful of scholars have examined the links between the two and the transition from the earlier to the later Salafis, however many works which impacted the evolution of Salafism at the dawn of the twentieth century have been neglected, especially those which do not align …
Hardware Trojan Detection Utilizing Graph Neural Networks And Structural Checking, Hunter Nauman
Hardware Trojan Detection Utilizing Graph Neural Networks And Structural Checking, Hunter Nauman
Graduate Theses and Dissertations
The integrated circuit (IC) industry has experienced exponential growth, particularly in the complexity and scale of hardware designs. To sustain this growth, faster development cycles and cost-effective solutions have been the focus for many companies. One strategy to maintain this growth is through the incorporation of third-party intellectual property (IP) into the IC design process. Outsourcing the production of sub-components reduces development time and enables faster time-to-market, however, this approach also introduces the threat of Hardware Trojans. Hardware Trojans, defined as any malicious modification or addition to an IC, pose significant security risks due to their small size, low activation …
Ripperature: Crossing Gender And Genre, Gracie M. Bain
Ripperature: Crossing Gender And Genre, Gracie M. Bain
Graduate Theses and Dissertations
The murders of Polly Nichols, Annie Chapman, Elizabeth Stride, Catherine Eddowes, and Mary Jane Kelly in London in 1888 by the serial killer commonly known as “Jack the Ripper” were grotesque displays of violence against vulnerable women. As unsolved crimes, they have continuously been sensationalized and fictionalized since they occurred, beginning with dime novels like The Whitechapel Murders; Or, On the Track of a Fiend (1888) and continuing with novels like Stalking Jack the Ripper (2016). The sheer number of adaptations is staggering. This extensive adaptation process results in the mythologization of Jack the Ripper as a culture-text. This study …
Talking About Aphasia: A Social Media Analysis Of What Individuals With Aphasia And Communication Partners Convey About Functional Discourse, Anna Williams
Graduate Theses and Dissertations
This study aimed to blend what we know about aphasia and functional communication. Specifically, how individuals with aphasia are functionalizing communication on the social media platform of YouTube. Extensive clinically significant literature exists on functional communication within speech-language pathology that provides practical guidance for clinical applications. Moreover, an emerging theoretical framework on functional communication and aphasia has sparked fresh insights, particularly from the perspective of individuals with aphasia. This perspective holds promise for exploring how individuals and their families leverage social media for functional communication. This study aims to integrate established clinical research findings on functional communication with the evolving …
Exploring The Governmental Intervention, Journalistic Approach, And Theories Involved In Reducing Poverty Journalistic For A Special Project – Feature Story On Non-Profit Organization Called Circles In Northwest Arkansas, Trevor Spencer
Graduate Theses and Dissertations
Over the 60 years since President Johnson declared the War on Poverty, with close to 40 million people currently in poverty nationwide as of 2022, government measures of the issue, like the Federal Poverty Level and Supplementary Poverty Measure, have continued to be significant determiners for who is constituted as poor. Yet, framing of the poor by sociologists has shifted to a more multi-dimensional approach to who constitutes as poor and how to help them. This means that the definition of poverty is beginning to include a larger set of deprivation unaccounted for in a nation where the definition of …
Well-Being Behaviors And Outcomes In Arkansas Teachers: A Qualitative Analysis Of Teachers In Arkansas During The Covid-19 Pandemic, Ashton Elizabeth Human
Well-Being Behaviors And Outcomes In Arkansas Teachers: A Qualitative Analysis Of Teachers In Arkansas During The Covid-19 Pandemic, Ashton Elizabeth Human
Graduate Theses and Dissertations
Introduction: The COVID-19 pandemic introduced new challenges to teachers and emphasized those already existing in the education system. The increased reliance upon technology and lack of administrative support perceived during distance learning led to lower levels of commitment to teaching and increased levels of teacher burnout. Burnout risk is associated with an increased workload, exhaustion from job demands, cynicism toward the professional community and has been shown to have a negative relationship with well-being. Teachers experiencing burnout are unable to establish positive relationships with students, understand students’ needs, stay in contact with trends in the education field, or provide pedagogically …
Navigating Transitions: Utilizing Autoethnographic Writing Prompts To Support Nontraditional College Students, Brijet Lynn Beazer
Navigating Transitions: Utilizing Autoethnographic Writing Prompts To Support Nontraditional College Students, Brijet Lynn Beazer
Graduate Theses and Dissertations
This study explores the transition nontraditional students undergo as they embark on their college journey and the unique challenges they face. Leveraging autoethnographic writing prompts as an intervention strategy, the project employed a participatory approach over virtual platforms to capture the tapestry of experiences and connect participants to form a supportive community. Participants included older individuals with work experience, some who experienced parenthood, or those returning after an extended break. Through crafted prompts, individuals shared their academic journey, balancing work, and family responsibilities. The reflections gathered provide valuable data, offering insights into the challenges and triumphs of nontraditional students. The …
Evaluating The Association Between Daily Fluctuations In Sleep And Blood Pressure, Mara Egeler
Evaluating The Association Between Daily Fluctuations In Sleep And Blood Pressure, Mara Egeler
Graduate Theses and Dissertations
Hypertension is a major contributing factor for heart disease, with nearly half of US adults meeting criteria. While studies have begun to examine the relationship between insomnia and blood pressure (BP), little is still known regarding the relationship between daily fluctuations in sleep and BP. This study aimed to provide further clarification on the role insomnia plays in the development of hypertension, including the impact that daily fluctuations in sleep have on fluctuations in BP. Participants included 61 adults from the greater Northwest Arkansas area (63.9% White, 67.2% Female, 30-79 y) who completed 10 consecutive days of sleep and blood …
Perceptions Of Families, Students, And Teachers On Iep Engagement For Families And Students Of Color During Junior High And Senior High School Iep Conferences, Amillia K. Oswalt
Perceptions Of Families, Students, And Teachers On Iep Engagement For Families And Students Of Color During Junior High And Senior High School Iep Conferences, Amillia K. Oswalt
Graduate Theses and Dissertations
This phenomenological study explored the experiences of families and students of color and their teachers during junior high and high school IEP meetings. This study, informed by DisCrit, explored the similarities and differences of families, students, and teachers during IEP meetings as well as the use of research-based practices designed to increase family engagement for culturally diverse families. Participants completed surveys and participated in interviews about their experiences. The student’s most current IEP was also reviewed to determine the ways in which teachers document family and student engagement within the IEP document. The major themes identified were that research-based practices …
Understanding Video Modeling Adoption: Perspectives Of Special Education Teachers, Support Specialists And Transition Consultants, Hayot Tuychiev
Understanding Video Modeling Adoption: Perspectives Of Special Education Teachers, Support Specialists And Transition Consultants, Hayot Tuychiev
Graduate Theses and Dissertations
This qualitative study investigates the knowledge, perceptions, and adoption factors of special education teachers and support specialists regarding video modeling (VM) as a vocational intervention for high school students with autism spectrum disorder (ASD). Grounded in Rogers' theory of innovation diffusion, the research explores their understanding of VM, its perceived effectiveness, and the factors influencing its adoption or rejection.This qualitative study investigates special education teachers' knowledge, perceptions, and adoption factors related to Video Modeling (VM) as a vocational intervention for high school students with autism spectrum disorder (ASD). Grounded in Rogers' theory of innovation diffusion, the research explores teachers' understanding …
Three Years Of Abrahamic Accords: A Contemporary Episode Of False Peace, Wael Abdelhafez
Three Years Of Abrahamic Accords: A Contemporary Episode Of False Peace, Wael Abdelhafez
Graduate Theses and Dissertations
The Abrahamic Accords mark the latest phase in the peace negotiation efforts concerning the Arab-Israeli conflict. Through diplomatic maneuvers, temptations, and promises, the United States managed to persuade four Arab countries to depart from the principles of the Arab Initiative and instead sign the Abrahamic Accords, establishing full normalization treaties with Israel. Many other countries and significant powers maintain divergent perspectives on the Abrahamic Accords. After three years, The Accords offer numerous potential economic, security, and strategic benefits, with Israel standing to gain the lion's share. This thesis explores the connection between the Frustration-Aggression Theory and the ongoing violence in …
An Inductive Analysis Examining Factors Contributing To Student Attrition In Introductory College Chemistry Courses And Proposed Solutions., Zephaniah Daniel Greenwell
An Inductive Analysis Examining Factors Contributing To Student Attrition In Introductory College Chemistry Courses And Proposed Solutions., Zephaniah Daniel Greenwell
Graduate Theses and Dissertations
A grounded theory study was conducted to investigate possible methods for reducing the number of “D”, “F”, or “W” grades (DFW rate) in the 1st semester general chemistry course. Classroom observations conducted over the 2022-2023 school year resulted in a 17 questions survey that was distributed in both the fall 2023 and spring 2024 semesters with 157 total student responses. While these surveys were being collected, an interview protocol was developed for students and instructors, who have experience teaching or taking an introductory chemistry course. These interviews were conducted in the early spring 2024 semester. Ten undergraduate students, six graduate …
Development Of Genomic Resources And Group Specific Markers For Evaluation Of Genetic Diversity Of Pathogenic Rhizoctonia Species, Juanita Gil Bedoya
Development Of Genomic Resources And Group Specific Markers For Evaluation Of Genetic Diversity Of Pathogenic Rhizoctonia Species, Juanita Gil Bedoya
Graduate Theses and Dissertations
Rhizoctonia is a complex genus in the phylum Basidiomycota comprised of several taxa distributed across different families in the order Cantharellales. Many species in the genus have been identified as plant pathogens, saprobes, or beneficial mycorrhizal symbionts of orchids. Rhizoctonia isolates are divided into binucleate and multinucleate and then classified into anastomosis groups (AGs) based on the ability of compatible hyphae to fuse. Binucleate Rhizoctonia species (Ceratobasidium spp.) are classified into 21 AGs identified with letters (AG-A to AG-U). Multinucleate Rhizoctonia species (Thanatephorus spp.) are identified with numbers and classified into 14 anastomosis groups (AG-1 to AG-13, and bridging group …
Standing On The Edge Of A Dream, Parto Ahmadpour Mobarake
Standing On The Edge Of A Dream, Parto Ahmadpour Mobarake
Graduate Theses and Dissertations
Standing On the Edge of a Dream delves into the intricate tapestry of lived experiences shaped by relocation, emphasizing the nuanced space that exists between reality and imagination. As an individual who has undergone the transformative journey of immigration, I recognize that the concept of relocation is like standing on the edge of a dream. This notion becomes a living structure, intricately woven with threads from our past, present, and future. My artistic exploration extends beyond my artworks, yet it remains deeply rooted in my personal narratives. The artworks in the exhibition continue to draw inspiration from personal memories and …
Assessing The Evaporation Method For Soil-Water Retention Curve Development And Comparison Of Soil-Water Characteristics Across Different Tillage Practices In A Furrow-Irrigated Corn (Zea Mays L.) System, Jeferson Prass Pimentel
Assessing The Evaporation Method For Soil-Water Retention Curve Development And Comparison Of Soil-Water Characteristics Across Different Tillage Practices In A Furrow-Irrigated Corn (Zea Mays L.) System, Jeferson Prass Pimentel
Graduate Theses and Dissertations
The thesis comprises three studies. The first study in this thesis (Chapter I) focused on a six-year corn field experiment to assess the influence of conservation agricultural practices on soil properties and their long-term effects on water-use efficiency and yield. Non-tillage management did not significantly reduce soil bulk density compared to conventional tillage, as no differences were observed between non-tillage and tillage systems throughout the 6-year experiment. There was no difference in total water-use efficiency among soil management practices in 2018, 2019, 2020, and 2021. Even though in one year of the study, a significant 24 kg ha-1 mm-1 improvement …
Development Of A Prototype Satellite-Tracking Telescope For Ground-Based Spectroscopy, Charles Smith
Development Of A Prototype Satellite-Tracking Telescope For Ground-Based Spectroscopy, Charles Smith
Graduate Theses and Dissertations
Spectroscopy is the practice of determining the chemical composition of a gas through analyzing the light that is filtered by the gas. Spectroscopy is commonly used by astronomers to deduce the atmospheric composition of planets. This process is done by initially measuring the spectrum of light from the star that the planet orbits, and then measuring it again once the planet is in between the observer and the star. The spectrum light that is filtered out is dependent on the chemical composition of the gas, and so this information can be used to determine the chemical composition of the atmosphere …
Advanced Jet Impingement Cooling For Power Electronics, Reece Whitt
Advanced Jet Impingement Cooling For Power Electronics, Reece Whitt
Graduate Theses and Dissertations
Convective heat transfer by jet impingement cooling offers a suitable solution for high heat flux applications. Compared to techniques that rely on bulk conduction in series with convection, direct liquid impingement reduces the thermal resistance between power device hot spots and the coolant. Using additive manufacturing processes, location specific jets can be integrated into high voltage power electronics systems. Custom manifolds can be designed to reduce pressure drop, while simultaneously fine-tuning the thermal management efficiency. Although capable of highly efficient cooling, static impingement devices must be designed for the worst-case cooling requirements for a transient power profile, possibly resulting in …
The Impact Of Eye-Movement Desensitization And Reprocessing On Self-Reported Test Anxiety In College Students, Renee Wilson
The Impact Of Eye-Movement Desensitization And Reprocessing On Self-Reported Test Anxiety In College Students, Renee Wilson
Graduate Theses and Dissertations
Test Anxiety (TA) has become a major concern for college students. Experiencing mental concern about test-taking perpetuated by physiological hyperarousal directly affects students’ ability to be successful in college. Eye Movement Desensitization and Reprocessing (EMDR) is a multi-faceted therapeutic approach that aims to process dysfunctional negative cognitions which originate with negative past experiences to an adaptive state. Developed by Francine Shapiro in 1989, the Adaptive Information Processing (AIP) model undergirds the 30-second, therapist-initiated, bi-lateral eye-movements. These movements identify and reprocess emotionally disturbing experiences, linked to an individual’s emotional expression, by deliberatively triggering a connected trauma network. This dissertation examined the …
The Use Of Culturally Relevant Pedagogy In Esol Classes, Beth A. O. Haddinger
The Use Of Culturally Relevant Pedagogy In Esol Classes, Beth A. O. Haddinger
Graduate Theses and Dissertations
This dissertation is organized into five distinct chapters addressing multiple aspects of learning and engagement as it relates to English Language Learners (ELLs) in the classroom. First, the problem of practice is identified and explored. We, as educators, must look closely at instructional and systemic issues, actionable elements, broader strategies of improvement, and leverage, which refers to the ability of a program to motivate students and to generate a sense of practical value in academics. The next aspect of this dissertation proposal reviews the existing research literature. This literature review focuses on the specific learning needs of ELL students. Specifically …