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The Use Of Inorganic And Organic Acids As Short Duration Antimicrobial Dips On Mitigating Pathogens Present On Commercial Chicken During Peri-Harvest, Dana Kristen Dittoe

Graduate Theses and Dissertations

With poultry being the most consumed meat in the United States, poultry processors must provide consumers with safe, wholesome products. As a consequence, poultry processors are faced with the challenge of reducing the presence of foodborne pathogens such as Salmonella spp. and Campylobacter jejuni among raw poultry products. Though multi-hurdle approaches using antimicrobials are placed throughout processing to reduce these pathogens, Salmonella and C. jejuni still persist among raw poultry. Thus, it was the objective of the current dissertation to investigate various antimicrobials, organic and inorganic acids, as short duration dips and sprays as means to reduce common pathogens (Salmonella, …

Degree Of Conservation Of Methionines Found To Be Oxidized In The Human Urinary Proteome, Alexis Hall

Graduate Theses and Dissertations

In previous work from this laboratory, methionine containing peptides from the human urinary proteome were examined by mass spectrometry for the degree of methionine oxidation to the sulfoxide form. While this demonstrated that many of the methionines detected were capable of being oxidized, the question of whether these methionines are important in the structure and/or function of the parent proteins came about. In some proteins, methionine oxidation has been linked to conformational changes and alteration of function and thus can serve as a mechanism for reversible regulation of activity. It is hypothesized that methionines which might serve a regulatory purpose …

The Use Of Social Media In English Teaching And Learning: Exploring The Perceptions And Experiences Of English As A Foreign Language Instructors, Waheeb Shadid Albiladi

Graduate Theses and Dissertations

The attention on using social media for educational purposes is increasing. Research shows that social media platforms are effective teaching and learning tools. The purpose of this study was to examine foreign language teachers’ experiences in using social media in the teaching and learning of English. Research Design: A qualitative research method was used for the data collection and analysis. Data were collected through a survey and in-depth interviews with foreign language teachers. The participants included 35 English teachers (15 for the interview, 35 for the survey) representing different educational levels, including public and private schools and foreign language institutions. …

Understanding Radiation Resistance In Head And Neck Tumor Xenografts Using Diffuse Reflectance And Raman Spectroscopy, Sina Dadgar

Graduate Theses and Dissertations

Each year, 800,000 new patients are diagnosed with head and neck squamous cell carcinoma (HNSCC), a majority of whom are treated with a combination of daily fractions of radiation and weekly chemotherapy sessions for up to seven weeks. Current methods to evaluate treatment response of individual patients are limited to anatomical measurements of tumor burden using CT scan or MRI 4-8 weeks after completion of treatment. However, earlier knowledge of radiation-response prior to or at early days after commencement of therapy can aid oncologist with escalating and de-escalating treatment plans for exceptionally non-responding and responding patients. Such a knowledge can …

Effects Of Long-Term Variation In Temperature On Reproductive Phenology In A Population Of Eastern Bluebirds (Sialia Sialis), Paul Pleiman

Graduate Theses and Dissertations

This study investigates the relationship between multiple temperature variables, to include annual and pre-lay date temperatures with first-egg and mean first-egg lay dates of the eastern bluebird at the Warner Parks in Nashville, Tennessee, USA. Data is collected by citizen scientists for the Eastern Bluebird Nesting Box Project while visiting artificial nest boxes throughout the park and recording observations made during the breeding season. Temperature data is retrieved from the Northwest Alliance for Computational Science and Engineering’s Parameter-elevation Regressions on Independent Slopes Model (PRISM) Climate Group, based at Oregon State University. The analyses showed no correlation between annual or pre-lay …

Anti-Inflammatory Properties Of Blackberry Phenolic And Volatile Compounds, Pauline Morin

Graduate Theses and Dissertations

Berries are known for many health benefits including anti-inflammatory properties that lower risks of chronic diseases. These properties have been linked to high concentrations of phenolic compounds, especially anthocyanins. However, the present study hypothesized that volatiles could contribute to the berries’ bioactive properties. Thus, the objectives of this research are to profile the phenolic and volatile composition of 16 blackberry genotypes harvested at the Fruit Research Center of the University of Arkansas and to evaluate the antiinflammatory capacities of three selected genotypes on inflamed cells. Phenolic and volatile profiles were evaluated using chromatographic techniques. The three genotypes A2528T, A2587T and …

Effect Of Feed Additives And Toxic Elements On Swine Growth Performance, Nutrient Digestibility, Immune Function And Reproductive Performance, Richard A. Mudarra Hernández

Graduate Theses and Dissertations

To evaluate the effects of peptide in combination of zinc oxide (Zno) or acidifiers (Exp 1), and gossypol (G) from cottonseed meal (CSM) ( Exp 2&3) on growth performance, complete blood cell counts (Exp 1, 2&3), nutrient digestibility (Exp 1), plasma gossypol (Exp 2&3) and semen quality (Exp 3), weaned pigs (Exp 1), growing gilts (Exp 2) and growing boars (Exp 3) were randomly allotted to dietary treatments. Treatments for Exp 1 during phase 1&2 were: (1) Positive Control (PC), formulated to meet NRC (2012) nutrient requirements; (2) Negative control (NC), fish meal was reduced to achieve -0.13% SID lysine; …

Association Mapping For Soybean (Glycine Max L. Merr.) Protein And Oil Content, Joseph Najjar

Graduate Theses and Dissertations

Soybean [Glycine Max (L.) Merrill] is an important economic crop because of its high content of seed protein and oil. Seed oil and protein content are quantitative inherited traits. The genetics of seed protein and oil levels have been extensively studied, with 367 QTL reported for protein and 475 QTL reported for oil to date. Validation of such QTLs, and identification of easily-automatable molecular markers around these QTL will aid the progression of breeding for such traits. The focus of this research was to discover novel and verify previously reported QTL related to protein and/or oil content via Genome-Wide Association …

Multiphoton Microscopy And Deep Learning Neural Networks For The Automated Quantification Of In Vivo, Label-Free Optical Biomarkers Of Skin Wound Healing, Jake D. Jones

Graduate Theses and Dissertations

Non-healing ulcerative wounds that occur frequently in diseases such as diabetes are challenging to diagnose and treat due to numerous possible etiologies and the variable efficacy of wound care products. With advanced age, skin wound healing is often delayed, leaving elderly patients at high risk for developing these chronic injuries. As it is challenging to discriminate age-related delays from disease-related chronicity, there is a critical need to develop new quantitative biomarkers that are sensitive to wound status. Multiphoton microscopy (MPM) techniques are well-suited for 3D imaging of epithelia and are capable of non-invasively detecting metabolic cofactors (NADH and FAD) without …

Evaluation Of Different Ensiling Methods On Storage And Feeding Value Of The Residual Material From Edamame Soybean Processing, Ellen Herring

Graduate Theses and Dissertations

Use of organic waste material by ruminant animals from food processing operations potentially reduces costs and reduces environmental issues from disposal of these residues. Therefore, 2 experiments were conducted to evaluate the storage and feeding value of residual from edamame soybean processing for ruminant animals. Two types of waste streams, waste during harvest time and waste from processing stored material, were ensiled (on a laboratory scale) using various methods and effects on post-ensiling nutritive value were examined. Material from both waste streams were ensiled either without wilting or after wilting; each moisture level was ensiled with and without an inoculant. …

Fate And Dissemination Of Salmonella Reading In Market-Age Turkeys At Processing Using Oral Gavage Challenge Model, Aaron Ashcraft

Graduate Theses and Dissertations

This study aimed to evaluate the fate and dissemination of Salmonella Reading (SR) in market-age turkeys using an oral gavage challenge model. One hundred twenty-eight-week-old commercial turkey hens were moved from commercial production to research facilities. Upon arrival, a combination of enrofloxacin, 10 mg/kg, and florfenicol, 20 mg/kg, were orally administered sequentially before comingled placement on fresh pine shavings. Turkeys were challenged with 108 cfu SR by oral gavage on days 4 and 7 post-placement. Subsets were subjected to simulated commercial processing on days 14 (n=40), 21 (n=40) and 28 (n=32) post-placement (corresponding to 10, 11, and 12 weeks of …

Biogeography Of The Bovine Respiratory Microbiome And Its Relationship With Bovine Respiratory Disease, Jianmin Chai

Graduate Theses and Dissertations

Bovine respiratory disease (BRD) is the most common and costly disease in the beef cattle industry, leading to high morbidity, mortality and huge economic loss. Despite the recent advances in vaccination and antimicrobial techniques, no significant health-improved outcomes have developed. Due to a deep investigation of the microbiome, respiratory microbiotas are known to have important roles for host health and disease. However, BRD specific pathogens have not yet been identified since they are found in both healthy and diseased animals. A systemic and comprehensive study of the biogeography of the bovine respiratory microbiome and its relationship with BRD is lacking …

Culturally Responsive Pedagogy And Writing Achievement For Native High School Students, Angelia Christine Greiner

Graduate Theses and Dissertations

This study focuses on the effects of culturally responsive pedagogy as it is implemented in a high school classroom with Native American students. This mixed methods case study collects, analyzes and synthesizes both quantitative and qualitative data within an overall formative and experimental design approach to measure the effects of culturally responsive pedagogy on students’ writing and their attitudes about writing. The study identified specific approaches to literacy instruction that hold promise for engaging Native students. Furthermore, findings from the study reveal the use of visual art as a particularly powerful tool that extends students’ meaning-making skills, leading to more …

Evaluation Of Selected Bacillus Direct-Fed Microbial Candidates In Reduced Energy Diets On Live Performance, Carcass Characteristics, And Foot Pad Dermatitis, Kyle Teague

Graduate Theses and Dissertations

Bacillus spp. are ubiquitous, Gram-positive, spore forming bacteria that are commonly recovered from the environment and gastrointestinal tract (GIT) of poultry. These spores are capable of withstanding harsh condition such as feed pelletization, which facilitates inclusion in poultry feeds. Once ingested by the bird, spores germinate into metabolically active vegetative cells which can produce extracellular enzymes which can hydrolyze otherwise indigestible components of the feed. Soybean meal (SBM) is the gold standard vegetable protein source for non-ruminant animals worldwide and is included in practically all poultry diets in the United States at levels ranging from 10 to more than 30%. …

Characterization Of Growth Patterns And Meat Quality Characteristics Of Four Commercial Broiler Strains In Small Bird And Large Bird Programs In The United States, Clay Johnny Maynard

Graduate Theses and Dissertations

Through current trends in animal protein consumption, the United States poultry industry has seen a drastic rise in production and popularity. Over the last few decades, poultry meat has surpassed both beef and pork production to become the most widely consumed animal protein. This rise may be accredited to an increased transition to more health-conscious consumers. As more consumers purchase poultry as a lean source of protein, the need for a superior quality product is of great interest to poultry integrators.

Relationships between commercial broiler lines have been well documented in previous years, but constant and intensive genetic selection in …

Functional And Physiological Role Of Extra-Hypothalamic Corticotropin Releasing Hormone Neurons In The Nucleus Of The Hippocampal Commissure In Regulation Of Stress Response, Hakeem Kadhim

Graduate Theses and Dissertations

Corticotropin releasing hormone (CRH) neurons located within the paraventricular nucleus (PVN) are known to be involved in regulation of stress responses. Recently, CRH neurons were identified above the PVN within the nucleus of the hippocampal commissure (NHpC) that located in the septum. We hypothesized that CRH neurons in the NHpC play a critical role in the stress response due to their rapid activation and could be a part of the traditional hypothalamo-pituitary-adrenal (HPA) axis. The dissertation addresses the role of 1) CRH expressing neurons in the NHpC compared with those within the PVN utilizing two different stressors, food deprivation (FD) …

The Effects Of Multiple Stressors On Stream Communities: The Convergence Of Drought, Nutrient Pollution, And Invasive Species, Robert Joseph Fournier Iii

Graduate Theses and Dissertations

Freshwater systems experience multi-faceted degradation from a variety of ecological and environmental stressors. Three common stressors in these systems, drought, nutrient pollution, and invasive species, have wide-ranging effects on stream population- community- and ecosystem dynamics. We have a broad understanding of how each of these stressors works to influence stream systems independently. However, we still know relatively little about if, and how, these stressors might interact when they co-occur. Though drought is a natural part of many stream systems, all three of these stressors can be exacerbated or facilitated by anthropogenic actions. Accordingly, as human population and resource use continue …

The Effect Of Epa And Dha On Skeletal Muscle Physiology And Energy Balance, Saeed Jebor Ghnaimawi

Graduate Theses and Dissertations

Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA) supplementation commonly suggested during pregnancy confers many health benefits on offspring and more recently improves fetal BAT development and induces myoblast trans-differentiation into adiopocytes through activating PPARs expression. Embryonic myoblast is a highly flexible cell showing great capacity to change its phenotype into adipocyte in response to EPA and DHA supplementation in many in vitro models. However, a little is known about the identity of derived adipocytes, whether, they change into brown or white adipocyte, and in vivo evidence is still missing. Given that the myogeneis process is initiated at the mid of …

Dietary Regulation Of Successful Aging, Aubree L. Hawley

Graduate Theses and Dissertations

The current growth of the older population is unprecedented in U.S. history. Chronic disease and functional limitation commonly develop prior to old age, leading to prolonged physical disability and decreased well-being. The development of chronic disease and loss of independence is associated with lean body mass (LBM) loss and fat mass gain beginning in middle age. Therefore, it is important to identify modifiable factors to mitigate deleterious shifts in body composition to promote successful aging (SA). The concept of SA is associated with longevity, the absence of disease and disability, and subjective components of well-being, however, an operational definition has …

Effects Of Alcohol Intoxication On Hostile Attribution Bias And Relational Aggression In Women, Alita M. Mobley

Graduate Theses and Dissertations

Alcohol intoxication is consistently linked to physical and sexual aggression in men, but not women. The lack of evidence supporting the relationship between alcohol and aggression for women could be due to a failure to measure relational aggression (i.e., harmful social manipulation), the form of aggression more commonly employed by women. Further, alcohol intoxication may interfere with the interpretation of social cues, resulting in greater perceived provocation in ambiguous social interactions and increased aggression. The current study examined the relationship between alcohol intoxication and relational aggression in women and the extent to which interpretation of social cues (i.e., hostile attribution …

Examining Pathogenesis And Preventatives In Spontaneous And Staphylococcus-Induced Bacterial Chondronecrosis With Osteomyelitis In Broilers, Nnamdi Simon Ekesi

Graduate Theses and Dissertations

Bacterial chondronecrosis with osteomyelitis is the most important cause of lameness in broilers. This is important to poultry production, as it poses animal welfare issues, and causes a significant loss in revenue. The remediation of this disease requires the study of its etiology with fitting models and evaluating preventatives. The research reported herein covers genomic virulence analysis of BCO isolates, mainly Staphylococcus aureus, and Escherichia coli retrieved from lame birds. We found that S. aureus isolates were closest to chicken strains in Europe but may have been in the Arkansas area for a decade. Phylogenomics suggest our S. aureus is …

Optimization Of In-Vessel Food Waste Composting: Enzyme Activity And Microbial Dynamics, Ayawovi Selom Ametepe

Graduate Theses and Dissertations

A series of greenhouse-based, rotary-drum bioreactor experiments was designed to study microbial dynamics and enzyme activity during optimization of food waste composting. This work aims to optimize food waste composting by defining the best food waste-to-bulking agent proportion controlling conditions and by evaluating the food waste composting process when inoculated by a bacterial inoculant product compared to uninoculated compost. Three experiments were run in total. The two first experiments were conducted for 48 days with sampling at each step of composting, while the third experiment last 50 days and included one extra sampling date. In the first experiment, 50:50, 65:35 …

Assimilating Through Consumption: A Rhetorical History Of The Early Years Of The Advocate, Cora Beth Butcher-Spellman

Graduate Theses and Dissertations

This thesis uses analysis of constitutive rhetoric and queer archival methods to examines how The Advocate used assimilationist rhetoric and consumerist rhetoric in fundamentally anti-democratic ways to consolidate the form of ideal gay consumer-citizenship. Focusing on the first three years of the publication, I utilize queer theory and theories of citizenship and political economy to explain how The Advocate’s rhetoric and mainstream success allowed the publication to normalize a limited and politically weak gay identity. This thesis argues The Advocate’s rhetoric of exclusion, authority, and consumerism were three central features shaping ideal gay consumer-citizenship as most available to people who …

Providence Lost: Natural And Urban Landscapes In H. P. Lovecraft's Fiction, Dylan Henderson

Graduate Theses and Dissertations

S. T. Joshi, the preeminent scholar of weird fiction, considers H. P. Lovecraft a “topographical realist,” noting that, in his later fiction, Lovecraft creates realistic and painstakingly detailed settings. In “Providence Lost: Natural and Urban Landscapes in H. P. Lovecraft’s fiction,” I explore the significance of Lovecraft’s topographical realism and trace its evolution through Lovecraft’s career. I argue that Lovecraft’s early fiction, the tales, that is, that he wrote from 1917 to 1924 under the influence of Edgar Allan Poe and Lord Dunsany, pays little attention to the natural landscape, though Lovecraft does, in story after story, allude to fabulous, …

An Impedimetric Aptasensing System For The Rapid Detection Of Salmonella Typhimurium, America Sotero

Graduate Theses and Dissertations

Salmonella Typhimurium is a foodborne pathogen associated with raw and undercooked eggs, poultry, beef, fruits, and vegetables. In the United States, Salmonella is responsible for approximately 1.2 million illnesses, 23,000 hospitalizations, and 450 deaths annually. For many years, conventional detection methods such as culture-dependent and PCR-based methods have been the “golden standards” for the detection of this pathogen due to their high sensitivity and reliability. However, they still have some disadvantages such as long enrichment steps and high costs that need to be overcome. The development of a rapid and reliable method for the detection of S. Typhimurium is needed …

Improving Breeding Program Efficiency And Genetic Gain Through The Implementation Of Genomic Selection In Diverse Wheat Germplasm, Dylan Larkin

Graduate Theses and Dissertations

Genomic selection (GS) is an important tool for increasing genetic gain for economically important traits in breeding programs. Genomic selection uses molecular markers across the entire genome in order to predict the performance of breeding lines for a trait of interest prior to phenotyping. A training population (TP) of elite germplasm, representative of the University of Arkansas wheat breeding program, was developed in order to predict important agronomic and Fusarium head blight (FHB) resistance traits within the University of Arkansas wheat breeding program through cross-validation and forward prediction.

A genome-wide association study (GWAS) was performed on the TP to identify …

Investigating The Potential Of Microbial Metabolites And Mtor Signaling On Chicken Necrotic Enteritis, Mohit Bansal

Graduate Theses and Dissertations

Necrotic enteritis (NE), caused by C. perfringens and coccidiosis, is responsible for substantial economic loss annually. In the first chapter, we investigated the effect of secondary bile acid on necrotic enteritis. Day-old broiler chicks were randomly assigned to 5 groups of diets supplemented with 0 (basal diet), 0.8, 1.0, and 1.5 g/kg (on top of basal diet) deoxycholic acid (DCA). The birds were challenged with Eimeria maxima (20,000 oocysts/bird) at d 18 and C. perfringens (109CFU/bird/day) at d 23 and d 24 to induce NE. Birds were sacrificed at d 26, and ileal tissue and digesta samples were collected. DCA …

The Surveillance Of Gastrointestinal Parasitic Nematodes Of Northwest Arkansas Dairy Cattle Using Traditional And Genetic Parasitological Identification Procedures, Eva M. Wray

Graduate Theses and Dissertations

Traditional and genetic parasitological identification procedures were compared using naturaland artificial nematode parasite infections in Holstein steer calves. The traditional parasitological procedures measured fecal egg counts, coprocultures with subsequent larval collection and adult nematodes collected at necropsy. The genetic identification procedures measured ITS-2 sequences extracted from different stages of nematode development: raw feces, concentrated nematode eggs, third stage larvae and adults. The primary nematodes observed were Cooperia oncophora, Cooperia punctata and Ostertagia ostertagi. The traditional techniques were not significantly different from one another, while the genetic sequencing showed variation amongst the different procedures. The raw feces sequences showed the most …

Effects Of Commercial Feed Additives On Growth Performance And Gut Microbiota Of Nursery Pigs, Xiaoyuan Wei

Graduate Theses and Dissertations

To secure animal welfare and maximum production, feed additives are often added to nursery pig diets to increase nutrition digestibility, enhance intestinal health, and prevent or mitigate disease, with the ultimate goal of improving growth performance. This thesis performed a longitudinal analysis using next-generation sequencing to investigate the dynamic changes of gut microbiota in weaned pigs fed commercial feed additives. Chapter II was used to evaluate the effects of organic acid mixture on growth performance and gut microbiota of weaning pigs. This study demonstrated that the inclusion of 0.035% (SBA0.035) or 0.070% (SBA0.070) sodium butyrate in a diet containing 0.5% …

Neogene And Quaternary Events Shaped Diversification And Speciation In Bhutanese Rheophilic Fishes Of The Family Nemacheilidae (Cypriniformes) And Sisoridae (Siluriformes), Karma Wangchuk

Graduate Theses and Dissertations

Biogeography of the Himalayan region [to include the Qinghai-Tibetan Plateau (QTP)] evolved over a ~30M year span, catalyzed by the collision of the Indian and Eurasian plates. The resulting uplift produced major ecological and climatic effects, that in turn drove the diversification of biodiversity. As a result, the QTP is designated as a global biodiversity hotspot particularly vulnerable to cumulative climatic effects, including shrinking distributions, declining numbers, and local extinctions. Understanding how the biodiversity within the Himalaya/ QTP was established and maintained is a necessary first step in prioritizing conservation efforts.

Fishes in global montane regions, such as the Himalaya, …