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Allopurinol Alters The Expression Of Cytokines And Mediators Of Immune Cells In The Systemic 2 Tissue By Decreasing Plasma Urate., Lundrim S. Marku

Graduate Theses, Dissertations, and Problem Reports (ETD)

Diminished levels of urate have been linked to oxidative stress in birds and mammals. Urate, a major antioxidant that lowers reactive oxygen/nitrogen species (ROS/RNS), is thought to ameliorate the effects. The relationship between reduced urate, the immune system and the pathogenesis of the intestine, the liver or the brain has not been well characterized in avians. Moreover, recent studies have proposed that an increased permeability in the intestine due to some insult can induce inflammation in peripheral organs such as the brain. Allopurinol, a relatively toxic purine analogue that serves as a xanthine oxidase inhibitor, reduces urate levels which can …

A Video Decision Aid For The West Virginia Post: A Randomized, Controlled Trial, Jarred Vicente Gallegos

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Physician Orders for Life Sustaining Treatment (POLST) allows patients to express preferences for end-of-life medical decisions. Although POLST forms are used throughout the United States, evidence is lacking about the quality of the decision-making process of individuals completing these forms. The use of a decision aid developed for the POLST could ensure that patients completing these forms are informed, confident, and certain of their treatment decisions. The purpose of the study was to develop and evaluate a video decision aid for the West Virginia POST. 64 English-speaking, community-dwelling adults (50+), with no evidence of cognitive impairment, were recruited to …

Characterization Of Ovine Monocytes In Response To Haemonchus Contortus Larvae In Vitro And A Novel Role Of Interleukin-13 Inducing Larval Paralysis, Elizabeth Shepherd

Graduate Theses, Dissertations, and Problem Reports (ETD)

Haemonchus contortus is a hematophagous, gastrointestinal parasite affecting small ruminants. Parasitism by H. contortus is the greatest concern of sheep producers in the United States and accounts for billions of dollars in economic loss worldwide. However, different breeds of sheep display differences n natural resistance to H. contortus. St. Croix hair sheep have a remarkable ability to clear H. contortus without the aid of anthelmintics and develop a robust T helper type 2 (Th2) immune response, preventing establishment of infective stage 3 larvae (L3), when compared to susceptible Suffolk sheep. However, the mechanism of development of immune response and …

Evaluating The Impact Of An Intervention To Promote Empathy For Patients With Substance Use Disorder In A Rural Emergency Department, Theresa Poling

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background

Negative attitudes of health professionals towards patients with SUD are common and contribute to suboptimal health care for this marginalized group of patients (Van-Boekel, Brouwers, Van-Weeghel & Garretson, 2013).

Objectives

Promoting empathy in the Emergency Department (ED) has the potential to increase nursing staff’s awareness of their attitudes and perceptions toward patients with SUD, thereby decreasing the stigma associated with addiction and improving patient care and patient outcomes. The rationale for implementing this project was to evaluate a tool to promote empathy. This tool was developed by the student researcher based on a literature search and analysis.

Design

This …

Utilizing The Parts Of Common Food Products As Functional Ingredients Part 1: Separation And Concentration Of Ω-3 Pufa-Rich Phospholipids By Hydration Of Krill Oil Part 2: Functional Properties Of A Concentrated Egg Yolk Protein Powder Using A One-Step Organic Solvent Extraction Process, Casey Lynn Showman

Graduate Theses, Dissertations, and Problem Reports (ETD)

The food industry separates food products into different parts to better utilize the functional and nutritional benefits of each fraction. The overall focus of this thesis is on separating and utilizing fractions and co-products from food manufacturing as functional ingredients. Therefore, the objective of this research was to 1) evaluate the effectiveness of water degumming to separate omega-3 PUFA-rich phospholipids from other lipids found in krill oil and 2) determine how different organic solvents would affect physiochemical and functional properties of egg yolk protein recovered from spray-dried egg yolk using a novel one-step organic solvent extraction method.

The aim of …

Soil Health Assessment In Reclaimed Mine Soils, Katie Danielle Stutler

Graduate Theses, Dissertations, and Problem Reports (ETD)

West Virginia is one of the top coal-producing states in the nation, which has resulted in over 500,000 acres of highly disturbed, anthropogenic mine soils caused by surface mining. Mine soils are often rocky and contain low organic matter content, low plant-available nutrients, and toxic metals, which could result in poor soil health and low productivity. Soil health has been defined as the continued capacity of soil to function as a living ecosystem that sustains plants, animals, and humans. It is assumed that with time since reclamation, mine soil properties and related functions may change, and soil health will improve …

Three Essays In Applied Econometrics, Collin Dean Hodges

Graduate Theses, Dissertations, and Problem Reports (ETD)

The body of work presented here consists of a collection of research projects developed during my time as a graduate student at West Virginia University. As will soon become apparent, this collection of research topics is quite eclectic. This is, in part, due to the to the nature of the research process itself--where one often begins by asking one question and ends by answering a completely different one--but also due to the fact that my time at West Virginia University has provided me the opportunity to gain insight into a wide variety of economic fields as well as work on …

Utilizing Lipid Biomarkers To Understand The Microbial Community Structure Of Deep Subsurface Black Shale Formations, Rawlings Akondi

Graduate Theses, Dissertations, and Problem Reports (ETD)

The deep subsurface environment has been known to host microbes as early as 1926 and has also been suggested to potentially account for as much as 50% of the Earth`s biomass. Researchers have shown that microbes alter their membrane lipid components in response to physiological stress, producing stress indicative lipid biomarkers. However, little effort has been made to understand the subsurface microbial community of the shale ecosystem which is increasingly being exploited and altered by addition of drilling and hydraulic fluids to meet our growing energy needs. Phospholipid fatty acids (PLFAs) are microbial lipid biomarkers and are found in all …

The Effect Of Bovine Galectin-1, A Conceptus Secretory Protein, On The Endometrial Transcriptome, Lindsay Faye Grose

Graduate Theses, Dissertations, and Problem Reports (ETD)

Early embryonic loss in cattle is detrimental to reproduction thus, the profitability of both the beef and dairy industries. Therefore, an important aspect of study is to find techniques that beef or dairy producers could use to decrease early embryonic loss Galectin-1, the protein that was investigated in this research is important in modulating the maternal immune system in rodent and human early pregnancies by promoting fetomaternal immune tolerance. The role of galectin-1 within the reproductive tissues has not been thoroughly investigated in cattle. Galectin-1 is located in the caruncular and intercaruncular regions of the bovine endometrium, specifically the maternal …

A Comprehensive Evaluation Of A Home Visitation Program Model's Prenatal Physical Activity Curriculum Modules Delivered Primarily To Low-Income Women, Angela Marie Dyer

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background. Many low-income pregnant women receive prenatal physical activity (PA) curricula modules through home visitation programs. However, there is a lack of scientific evidence on the structural capacity, processes, and outcomes relating to these modules. Aims. The objective of this dissertation project was to examine the aforementioned in a single home visitation program model - the Maternal Infant Health Outreach Worker (MIHOW) program. More precisely, the aims of this study were to: (Aim 1) examine home visitors’ (i.e., Outreach Workers [OWs]) competencies in implementing the MIHOW program’s prenatal PA curriculum modules (i.e., structural capacity), (Aim 2) …

Improving The Care Of Infants With Neonatal Abstinence Syndrome By Implementing Rooming In, Tammi M. Clutter

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Opioid use and drug abuse has led to an increase in fetal exposure to illicit drugs in the United States, putting these infants at risk for developing neonatal abstinence syndrome (NAS). Parental involvement in the care of these infants during their hospitalization has become an essential part of the treatment process

Purpose: The purpose of this project is to implement rooming in for infants with in utero exposure to opioids as a quality improvement practice change.

Methods: A literature search relating to improving the care of infants with NAS was conducted. Educational handouts were provided to staff regarding the …

Determining The Role Of Nudt7 In The Regulation Of Cellular Coa Levels And Metabolism, Stephanie Anne Shumar

Graduate Theses, Dissertations, and Problem Reports (ETD)

Coenzyme A (CoA) is an essential cofactor required for hundreds of metabolic processes. Because it is such a critical cofactor, CoA levels are tightly regulated. In the fasted state and in diabetic mice, the concentration of CoA increases dramatically in the liver. This phenotype is associated with constitutively low CoA degradation, a process that is emerging as a potentially important mechanism for CoA regulation. Nudt7 and Nudt19 are two mammalian peroxisomal enzymes with CoA-degrading activity, which are highly expressed in the liver and kidney, respectively. Limited information is available on the biochemistry of Nudt7 and Nudt19; the structural basis for …

Characterization Of Nodule-Specific, Slac And Mate Membrane Transporters In Medicago Truncatula, Lina Yang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Legumes play a crucial role in sustainable agriculture because of their intrinsic ability to reduce atmospheric N₂ into NH₃ via symbiotic nitrogen fixation (SNF). SNF is carried out in the symbiosome, a quasi-organelle containing endosymbiotic rhizobial bacteria in the cytoplasm of infected cells of the nodule. Therefore, the bacteria are surrounded by a symbiosome membrane (SM), which is derived from the plant plasma membrane during infection. SNF requires constant nutritional exchanges between symbionts, including reduced carbon (dicarboxylates) from the plant for reduced nitrogen (NH₄⁺) from the bacteroids. This exchange of nutrients and signals is fundamental …

Evaluating The Effects Of Exogenous Enzyme Supplementation On Broiler Growth, Victoria E. Ayres

Graduate Theses, Dissertations, and Problem Reports (ETD)

Experiments were conducted to investigate the effects of exogenous enzymes in poultry diets. In Chapter 4, a study was conducted to determine the effects of a corn-expressed recombinant carbohydrase (AC1) on broiler performance and digesta viscosity in high non-starch polysaccharide (NSP) diets through 21 days of age. One day-old Hubbard × Cobb 500 chicks were assigned to 6 dietary treatments. Each treatment consisted of 12 replicate pens of 10 birds. The positive control diet (PC) was a corn and soybean meal formulation. The negative control diet (NC) included 10% wheat and 10% corn distiller’s dried grains with solubles (DDGS). The …

An Assessment Of The Methods Used To Teach Content Skill Sets Required For The Introduction To Agriculture, Food, And Natural Resource Course, Lee I. Wright

Graduate Theses, Dissertations, and Problem Reports (ETD)

Numerous studies have shown that students learn best when theory is applied to hands-on experiences. Traditionally, vocational agriculture, which evolved into agricultural education, has incorporated hands-on learning. For example, students typically raise livestock and crops as part of supervised agricultural experiences; learn to use chain saws, tractors, hay rakes, balers and other equipment found on farms; and basic metal working skills. They also sample soil and water to test for mineral levels and types of pollution that might affect agricultural practices. The purpose of this study, which incorporates a survey of agricultural education teachers in West Virginia, is to determine …

Characterization Of Major Flavonols In Ramps (Allium Tricoccum), Wijdan M. Dabeek

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ramps (Allium tricoccum) is a traditional plant in the eastern Appalachian Mountains. Ramps have been used in Appalachian folk medicine for its health promoting roles in lowering blood pressure and cholesterol. Information on the chemical composition of bioactive components responsible for the potential health benefits are scarce. Analysis of the chemical composition in similar Alliumplants showed that flavonols, induced similar health benefits. Therefore, the aim of this work is to characterize major flavonol conjugates in ramps. The secondary aim is to develop a preliminary quantification protocol. Flavonol glycosides were extracted in 50% methanol and 3% acetic acid. …

Ferritin: An Iron Deficiency Biomarker In Collegiate Female Distance Runners, Ty Bigelow

Graduate Theses, Dissertations, and Problem Reports (ETD)

Context: Iron deficiency is a concern in distance runners due to the implications that hemoglobin production becomes impaired limiting oxygen uptake and tissue oxidation in the body. Ferritin is a biomarker used to diagnose iron deficiency. The related signs and symptoms of iron deficiency are associated with decreased performance and prolonged recovery. Objective: The purpose of this study was to investigate whether the change in ferritin levels affect measures of performance, measures of recovery, and rate of perceived exertion in female endurance runners. Design: The study was a descriptive longitudinal study. Setting: The study was conducted at a Mid-Atlantic Division …

The Role Of Sirt1 In Skeletal Muscle Function And Repair Of Older Mice, Matthew John Myers

Graduate Theses, Dissertations, and Problem Reports (ETD)

Human skeletal muscle is a highly metabolic tissue necessary for mobility and coordination. Responsible for approximately one-fifth of the resting human metabolism, skeletal muscle is also an important regulator of metabolites like glucose and contributes to the regulation of body temperature. Although there is a gradual decline in muscle mass associated with aging, a certain percentage of the population suffer from severe muscle mass and strength deterioration, classified as sarcopenia (5-13% for people aged 60-70 years old, 11-50% for those 80 or older). Sarcopenia is linked to increased morbidity and mortality rates in the elderly population, while annual healthcare costs …

Evaluating The Potential Of Methyl Jasmonate Application As An Agricultural Practice On Phytochemicals In Brassica Vegetables: Sensory Quality, Cooking, And Cost-Benefit Analysis, Yu-Chun Chiu

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the United States, Brassica vegetables, including broccoli (Brassica oleracea var. italica) and kale (Brassica oleracea var. acephala or Brassicae napus var. pabularia), are widely consumed and are easily accessible in farmer’s market or grocery stores with increasing interest of their health-promoting properties. For example, the consumption of broccoli has been associated with anti-cancer activity in in vitro and in vivo trails due to the high content of phytochemicals, minerals, vitamins, and fibers. Application of methyl jasmonate (MeJA) has been reported to enhance the potential health-promoting compounds in Brassica vegetables, glucosinolates (GS), especially indolyl GS neoglucobrassicin …

Genetic Regulation Of The Elicitation Of Glyceollin Biosynthesis In Soybean, Md. Asraful Jahan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Glyceollin phytoalexins are the pathogen-elicited defense metabolites that belong to the isoflavonoid family of molecules of soybean (Glycine max L. Merr). Phytophthora root and stem rot of soybean caused by Phytophthora sojae is a destructive disease throughout the soybean-growing regions worldwide causing devastating economic damages (globally $1-2 billion and over $250 million in the USA) every year. Engineering soybean plants that produce higher levels of glyceollins could confer resistance against this pathogen. Glyceollins also show anticancer and neuroprotective activities in mammals, therefore they are important for agriculture and medical research. Firstly, we were interested in understanding how biotic and …

The Effect Of Occlusal Pressure On Vertical Force Generation On Dentate And Edentulous Subjects, Matthew Thomas Harper

Graduate Theses, Dissertations, and Problem Reports (ETD)

Objectives: To determine 1) the difference in lower extremity power obtained from a vertical jump in individuals who are dentate or edentulous (implants or dentures) and 2) the difference occlusal pressure makes on lower body force output. Variables included jump height, maximum kicking force, maximum push off force, take off velocity, flight time, jump impulse, and maximum velocity. Groups included dentate, implant overdenture, and conventional denture.

Methods: Counter movement jumping with force plate collection was utilized to test the three populations in this study. The three populations were dentate, mandibular implant overdenture opposing maxillary complete denture, and conventional maxillary and …

Organizational Models In Athletic Training And The Effect On The Quality Of Care Delivered, Matthew Ferreira

Graduate Theses, Dissertations, and Problem Reports (ETD)

Context: Quality of care is an ethical priority to all healthcare professionals. Healthcare fields such as nursing and physical therapy have made changes in staff size and focus that have increased the quality of care provided to patients. The athletic training field has not made changes to the organizational structure. Thus, the quality of care delivered by athletic trainers is an understudied topic. Likewise, the organizational models have just recently been explored in the literature. Objective: Identify differences in the three models of organization (academic, athletic, and medical) related to quality of care delivered and coverage versus care. Design: This …

Learn First, Practice Second Approach To Increase Health Professionals’ Nutrition-Related Knowledge, Attitudes, And Self-Efficacy., Madison E. Santella

Graduate Theses, Dissertations, and Problem Reports (ETD)

The aim of this study was to assess the effectiveness of a “learn first, practice second” intervention on the nutrition-related knowledge, attitudes, and self-efficacy of multidisciplinary health professionals in West Virginia, specifically highlighting the Mediterranean Diet. Data was captured via online surveys at four time points (Baseline, Post-education, Post-immersion, Follow Up). All information was self-reported. This approach consisted of 16 weeks of online education, 2 weeks of cultural immersion in Tuscany, Italy, and a 7 month return to practice period. Data were analyzed using JMP Version Pro 12.2 and SAS Version 9.3 for Windows. Repeated measures ANOVA with irregular spacing …

Service Dogs For Veterans With Ptsd: Taxonomy, Work Stress Reduction, And Matching, Lindsay Parenti

Graduate Theses, Dissertations, and Problem Reports (ETD)

Research suggests that many veterans with post-traumatic stress disorder (PTSD) struggle with reintegration (Sayer et al., 2010), but are unlikely to seek help or complete treatment (Schottenbauer et al., 2008). To make matters worse, available treatment options are often time consuming, challenging, and/or associated with negative side effects (Carafano & Hutchinson, 2017). Using animals as a treatment modality for veterans with PTSD is an emerging topic of interest and has shown promise (Owen, et al., 2016; Richie et al., 2016). However, several factors have hindered the advancement of this field. Obstacles include a lack of standard terminology and classification system, …

Morphological And Functional Evidence Of Sexual Dimorphism In The Retrochiasmatic Area Population Of Nk3r-Containing Neurons In Sheep, Justin A. Lopez

Graduate Theses, Dissertations, and Problem Reports (ETD)

Neurokinin B (NKB) and kisspeptin are critical for fertility and potent stimulators of gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH) secretion in sheep. We previously demonstrated that administration of NKB receptor (NK3R) agonist, senktide, within the retrochiasmatic area (RCh) resulted in surge-like release of LH and increased expression of kisspeptin neurons in the arcuate nucleus (ARC) of female lambs. We concluded that NKB-responsive neurons in the RCh stimulate GnRH secretion by inducing kisspeptin release from neurons in the ARC.

We have demonstrated morphological and functional evidence of sexual dimorphism in NKB-NK3R signaling in ovine RCh. RCh senktide administration resulted in …

The Effect Of Sodium Selenate Biofortification On Plant Yield, Mineral Content, And Antioxidant Capacity Of Culinary Herb Microgreens, Rachel G. Newman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Selenium (Se) biofortification of plants has been suggested as a method for enhancing dietary Se intake. Popular herbs such as basil (Ocimum basilicum), cilantro (Coriandrum sativum), and scallions (Allium fistulosum) are used for enhancing flavors. Microgreens are young seedlings harvested with one to two true leaves and are increasingly popular in the consumer marketplace. In this study, basil, cilantro, and scallion microgreens were treated with various concentrations of Se as sodium selenate in hydroponic conditions to investigate the effect on plant yield, mineral content (selenium, sulfur, sodium, potassium, phosphorus, calcium, magnesium, copper, iron, manganese, zinc, and boron), total phenolic compounds, …

The Effect Of Feed Manufacture Techniques On Pathogen Reduction And Feed Nutrient Value, Timothy Paul Boltz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Experiments were conducted to evaluate how differing feed manufacture techniques, such as varying conditioning times and new equipment, can affect pathogen mitigation, feed manufacture metrics, amino acid digestibility, and enzyme recovery in feed. Feed hygienics are of increasing importance in providing safe feed to animals, and ultimately safe food for consumers. Salmonella has been identified as a major microbial hazard in animal feed that has been linked to animal and human illness. In chapter 2, the effects of standard pelleting and more thermally aggressive techniques were utilized to determine pathogen mitigation potential. Use of antibiotics has decreased in recent years …

Wish4campus: Evaluating College Food Insecurity And Promoting Solutions For Student Wellbeing, Rebecca L. Hagedorn

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: Interest in college food insecurity has increased in previous years, however, little research focuses on the Appalachian and Southeastern regions of the United States resulting in limited understanding of food insecurity’s impact on college students in these regions. Additionally, resources to help food insecure students are often sparse with universities lacking evidence-based programming to implement for student benefit.

Aims: This dissertation aims to (1) investigate the correlates and behavioral consequences of food insecurity on college students at an Appalachian university, (2) expand college food insecurity research to a regional investigation in the Appalachian and Southeastern regions, and (3) develop …

Apple Pomace As A Novel Aid For Western Diet-Induced Nonalcoholic Fatty Liver Disease In Young Female Sprague Dawley Rats, R. Chris Skinner

Graduate Theses, Dissertations, and Problem Reports (ETD)

Apple pomace is a “waste” byproduct of apple processing that causes environmental pollution and is costly to dispose of. Yet, apple pomace is rich in dietary fibers and antioxidants. Analysis of apple pomace’s nutritional profile indicates suitability as a potential dietary treatment for non-alcoholic fatty liver disease (NAFLD) and the more severe non-alcoholic steatohepatitis (NASH). NAFLD is the most prevalent liver disease in the world with prevalence and severity expected to increase in both adults and children. Currently, there is no approved drug treatment for NAFLD and therefore, dietary intervention is the primary treatment. The study objectives were to determine …

West Virginia University’S Changing Mission In The 21st Century, Katlin Swisher

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the midst of the ongoing shifts of the perception of higher education and the public’s question of the role and value of public universities, West Virginia University has simultaneously experienced institutional change through five different presidential administrations in a decade. As seen through WVU’s historical context as the state of West Virginia’s land-grant, flagship, research university, the institution has and continues to face many administrative, policy, and contextual changes as it strives to meet its strategic goals and operationalize its mission. Through document analysis and interviews with key officials, this qualitative, multi-case study seeks to understand how WVU’s mission …