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Genetic Monitoring And Rescue In Mid-Atlantic Brook Trout (Salvelinus Fontinalis) Poulations, Zachary Lorn Robinson

Graduate Student Theses, Dissertations, & Professional Papers

Brook trout (Salvelinus fontinalis) populations have experienced dramatic declines throughout their native range, in part, due to anthropogenic land use and habitat fragmentation. In the mid-Atlantic region, brook trout populations often occupy small, headwater habitat fragments in demographic and genetic isolation, making them vulnerable to inbreeding and genetic drift. My dissertation evaluates different methods for genetic assessment, monitoring, and management of small, isolated brook trout populations. First, I examined the potential value of effective number of breeders (N_b) estimates for genetic monitoring by determining whether N_b estimates were sensitive to habitat characteristics known to affect …

Bacteriophage Infection Induces Intracellular Polyamine Accumulation As A Defense Mechanism In Pseudomonas Aeruginosa, Camilia Dornelles De Mattos

Graduate Student Theses, Dissertations, & Professional Papers

Cells are constantly monitoring their extracellular environment for danger signals that warn of imminent threats. When cells are killed by physical or other non-infectious injuries, they release concentrated intracellular molecules into the surrounding area. These danger-associated molecules alert other cells that danger is nearby. When cells are killed by pathogens, they release both damage-associated and pathogen-associated signals. Both eukaryotic and bacterial cells sense these molecules and make threat assessments of cellular injury. Here, I show that lysis of Pseudomonas aeruginosa and other bacterial species releases high concentrations of polyamines that are in turn taken up by surviving cells in a …

Juvenile Temperature Regulation In Apis Mellifera (Honey Bee) And The Impacts Of Brood Temperature Requirements On The Colony, Scott Debnam

Graduate Student Theses, Dissertations, & Professional Papers

Little is known about the energetic costs to insects of raising young. Honey bees collectively raise young, or brood, through a series of complex behaviors that appear to accelerate and synchronize the timing of brood maturation. These include maintaining the brood nest at warmer and consistent temperatures and the exceptional activity of "heater bees." The temperature at which juvenile insects are raised can profoundly affect their development. Apis mellifera (Honey bees) cope with temperature-dependent development via social behavior that maintains the relatively high and constant temperatures within the nest where the brood are raised. Yet juvenile honey bee development is …

Understanding The Utilization Of Woody Biomass Through The Perspectives Of Southwest United States Forest Service Land Managers: A Qualitative Study, Mary-Ellen Reyna

Graduate Student Theses, Dissertations, & Professional Papers

Dry ponderosa pine/mixed conifer stands in the Southwestern United States create an overabundance of woody biomass during restoration and fuel treatments. It has been the job of land managers and resource specialists to develop management goals and practices to treat stands and lower the risk of catastrophic wildfires while managing for accumulations of woody biomass. Knowing the limitations, setbacks, and successes will help researchers, the United States Forest Service, and other land managers better improve woody biomass utilization. In conjunction with three previous ForBio Southwest studies, we present results from ten phone interviews from three Arizona and New Mexico ranger …

Looking To The Future Of Wildlife Conservation: Durable Wildlife Policy For The 21st Century, Charlie R. Booher

Graduate Student Theses, Dissertations, & Professional Papers

Wildlife conservation in the United States was built by the dollars of consumptive users. Monies from the sale of hunting licenses, as well as excise taxes on firearms, ammunition, and archery tackle through the Pittman-Robertson Federal Aid in Wildlife Restoration Act (PR), currently fuel a complex system of wildlife conservation via multiple levels of government. However, the number of hunters in this country is rapidly declining, the sale of firearms and ammunition is increasingly unrelated to hunting, and contemporary consumers tend to express different values than traditional hunters. These changes pose significant challenges of relevancy and funding to state and …

24-Hour Total Energy Expenditure During Ultra-Endurance Cross-Country Cycling, Patrick S. Dodds

Graduate Student Theses, Dissertations, & Professional Papers

ABSTRACT

Previous studies have used the doubly labelled water (DLW) method to evaluate the total energy expenditure (TEE) during Ironman, ultra-marathon trail runs and competitive road cycling. However, the technique has not been applied to a 24-hour cross-country mountain bike event. This case study examined the TEE, cycling metrics and ad libitum nutrient/fluid intake in a trained male cyclist during a 24-hour cross-country mountain bike race. A trained male cyclist (41 y, 74.1 kg, 172.4 cm) received an oral dose of DLW prior to the 24-hour event for the calculations of TEE and water turnover (rH₂O). Nude body …

A Machine Learning Algorithm Improves Surface Freeze-Thaw Classification, Fredrick Bunt

Graduate Student Theses, Dissertations, & Professional Papers

The frozen or thawed state of the land surface is an important factor affecting a wide range of natural processes such as surface water movement, the carbon cycle, and ecosystem development. It is also important for human endeavors such as permafrost engineering and agricultural planning. This makes having an accurate record important. The Freeze-Thaw (FT) Earth System Data Record (FT-ESDR) is a global, daily product that strives to be a reliable record of the FT ground state. In its current form, the FT-ESDR uses annual regression analysis of reanalysis surface air temperatures (SAT) and brightness temperatures (Tb) at each grid …

Causes And Consquences Of Fire In Forest Ecosystems Of The Selway-Bitterroot Wilderness, Melissa Jaffe

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

A Novel Pathway For The Production Of Poly-N-Acetylglucosamine By Borreliaceae, Adam Matthew Drobish

Graduate Student Theses, Dissertations, & Professional Papers

Poly-N-acetylglucosamine (PNAG) is an extracellular polysaccharide consisting of β-1,6 N-acetylglucosamine monomers. PNAG is a key component of biofilms produced by diverse bacteria including Escherichia coli, Staphylococcus aureus, Yersinia pestis, Bacillus subtilis¸and many more. The structure of PNAG is highly conserved across the microbial world. Here, we demonstrate that PNAG is produced by two Borrelia species, Borrelia (Borreliella) burgdorferi and Borrelia hermsii. PNAG production in B. burgdorferi, the Lyme disease spirochete, depends on pngA (bb0620), a gene annotated to express a glycosyl hydrolase family 3 (GH3) enzyme, which is a novel pathway. Surprisingly, in B. burgdorferi, PNAG appears to be intracellular, …

The Effects Humidity & Temperature Has On Dna Contamination During Storage, Samantha Leigh Allison Ramey

Graduate Student Theses, Dissertations, & Professional Papers

Studies have been conducted on DNA cross-contamination throughout the criminal investigation process in order to evaluation the possibility that DNA from one sample could potentially influence the outcome of another. However, no published studies have examined the potential for contamination during the storage of genetic evidence after samples have been taken from crime scenes or suspects. This study is a continuation of a preliminary project, which examined storage drying time in relation to cross-contamination. The current study tested temperature and humidity for the potential of cross-contamination during storage prior to extraction. Prior to storage, 50 μl of male saliva was …

Linear Programming Analysis And Diet Breadth Modeling At Bridge River, British Columbia, Sean Patrick Boyd

Graduate Student Theses, Dissertations, & Professional Papers

Studies in diet breadth modeling and patch choice have been and continue to be a hot topic of interest among practitioners of human behavioral ecology and the set of data available at Bridge River can certainly add to these debates and discussions that have been dominating anthropology in the past few decades. The faunal assemblage of Housepit 54’s 17 anthropogenic floors have provided researchers with a plethora of data that clearly indicates periods of resource depletion and partial to full site abandonment. Using Linear Programming and Diet Breadth Modelling I analyze the most represented species in the record and establish …

Facilitating Aquatic Invasive Species Management Using Satellite Remote Sensing And Machine Learning Frameworks, Sean C. Carter

Graduate Student Theses, Dissertations, & Professional Papers

The urgent decision-making needs of invasive species managers can be better met by the integration of biodiversity big data with large-domain models and environmental data products in the form of new workflows and tools that facilitate data utilization across platforms. Timely risk assessments allow for the spatial prioritization of monitoring that could streamline invasive species management paradigms and invasive species’ ability to prevent irreversible damage, such that decision makers can focus surveillance and intervention efforts where they are likely to be most effective under budgetary and resource constraints. I present a workflow that generates rapid spatial risk assessments on aquatic …

He, She, They, Other: An Examination Of Gender Associations With The Chatelaine In The Anglo-Saxon Culture, Dane A. Williams

Graduate Student Theses, Dissertations, & Professional Papers

The purpose of this paper is to study the chatelaine as a marker of gender attribution and overall usage within the Anglo-Saxon culture. Chatelaines are artifacts used to suspend multiple items to be employed for such purposes as grooming, tools, or keys and have been used widely from the Roman occupation of England during which it was used by all genders, to the Ninth Century when it was primarily used by women. As such, it is asserted that a single artifact should not to be solely relied upon to assign a gender identity to a burial, that these should be …

Conservation In Ecuador's Sangay-Podocarpus Connectivity Corridor: A Study Of Coproductive Capacities, Gabriel Isaac Oppler

Graduate Student Theses, Dissertations, & Professional Papers

Connectivity conservation, a growing topic across science, policy, and management, is considered an important supplement to protected areas. A diversity of ecological corridors is emerging to restore fragmented landscapes, preserve intact habitats, protect biodiversity, and improve resilience to climate change. Despite increasing expertise and resources for planning corridors, many initiatives face challenges mobilizing science and governance to implement corridors on the ground. In many cases, biophysical science is supplemented with social science to better understand the human elements of connectivity. Similarly, collaboration and partnership are heralded as essential for effective governance of corridors. However, less is known about how these …

An Integrative Investigation Of The Synechococcus A/B Clade During Adaptive Radiation At The Upper Thermal Limit Of Phototrophy, Christopher L. Pierpont

Graduate Student Theses, Dissertations, & Professional Papers

Thermophilic microorganisms have been scientifically observed since the early nineteenth century and have spurred many questions about the limits of life and the capacity of organisms to survive extreme conditions. Decades of research on thermophile proteins and genomes have yielded several proposed correlates of temperature that may contribute to adaptation of bacteria and archaea to high temperature. However, many of the generalizations reported are drawn from analyses of deeply divergent taxa or from individual case studies in isolation from mesophilic relatives. Members of the Synechococcus A/B (SynAB) group are the only cyanobacteria with members able to grow above 65 °C …

The Effect Of Attentional Manipulation On Cough Reflex Sensitivity In Patients With Cough Hypersensitivity Syndrome, Jane Elizabeth Reynolds

Graduate Student Theses, Dissertations, & Professional Papers

The cough reflex is complex and requires integration of sensorimotor information from the central and peripheral nervous system. Cough motor output can be volitionally modified even when a strong urge-to-cough (UTC) is felt which reinforces the idea of cortical involvement in cough and opens the door to consider how manipulating attention or changing cognitive load may impact cough reflex sensitivity. Two prior studies have shown healthy individuals have reduced cough reflex sensitivity to inhaled tussigenic stimulants when completing a distracting task, presumably due to influence from increased cognitive load. In this prospective case series study, 13 patients with cough hypersensitivity …

Development Of Nanodisc Affinity Capillary Electrophoresis To Study The Interaction Of Giα Protein With Cell Membranes, Bethany R. Emeigh

Graduate Student Theses, Dissertations, & Professional Papers

Guanine nucleotide-binding proteins, or G-proteins, are crucial to cell signaling by acting as molecular switches through interactions with membrane-bound receptors. G-proteins have become of greater interest due to the possibility of novel drug targets. The G-protein alpha subunit, Gα, binds GTP before localizing to the membrane to interact with other G-protein subunits and G-protein-coupled receptors (GPCRs). Gi lacks a hydrophobic region to promote localization to the membrane, but a co-translational lipidation of the N-terminal residue stabilizes this interaction.

Protein interactions with biological membranes are challenging to study due to the complex structure of membranes and the often-limited quantities of the …

Getting To Know You: Relationship Formation Across Development In Octodon Degus, Amber Lynn Thatcher

Graduate Student Theses, Dissertations, & Professional Papers

Social relationships are a necessary part of group formation and cohesion across many animal species and can increase the health and fitness of the individuals involved. These benefits are seen across the lifespan, although the exact advantages and functions differ across developmental stages. Our current understanding of how relationships are formed and maintained is incomplete. Previous findings suggest that a measure of stability may be a useful method for understanding how relationships are formed and how this process may differ across development. The goal of this dissertation is to investigate the variability of social interactions over time and how it …

The Birds, The Bees, The Trees, And The Breeze: Expanding The Moral Universe Throught Rsk Anlysis, Jared Gibbs

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

Preliminary Study In More Effective Utilization Of 87sr/86sr Analysis In The Field Of Forensic Anthropology, Samantha Powers

Graduate Student Theses, Dissertations, & Professional Papers

Isotopic analyses are gaining attention in the field of anthropology. The current utilization of strontium isotopes primarily focuses on residence and migration patterns of past populations extracting data from tooth enamel to indicate early-life residence This study aims to identify a skeletal feature to be set as a field standard in strontium analysis of modern remains when seeking end-of-life residence, the impact of diagenesis on modern remains, and to test the accuracy of the petrous as an alternative to tooth enamel.

The results of this research were unable to suggest such a standard but gathered helpful data to be used …

Re-Storying Grant Creek: A Case Study Of Relational Dynamics On A Degraded Montana Stream, Seamus Rucci Land

Graduate Student Theses, Dissertations, & Professional Papers

The UN Decade on Ecosystem Restoration began in 2021, and after a history of contentious ethical debates, ecological restoration is increasingly portrayed as a viable framework for combating environmental degradation and supporting more healthy and stable social-ecological systems. The proposed ecological restoration of Grant Creek, a degraded stream near Missoula, Montana, offers an opportunity to connect a restoration site to the broader, rapidly growing field of restoration practice. It also allows the opportunity to forward the ‘relational turn’ proposed by many in the sustainability sciences as an ontological and methodological means to move beyond positivist portrayals of social-ecological systems, which …

Other Orchards, Sam B. Robison

Graduate Student Theses, Dissertations, & Professional Papers

Other Orchards is comprised of poems each suspect in their own way of those boundaries that might separate humans from nature, rural from urban, worker from scholar, or human from beast. Using the figure of the orchard, a kind of “false forest,” this collection studies the ways we map ourselves onto our work and the way work might inform an understanding of the self. Ultimately, these are poems that emerge from the seams of things—the shoulder of highway strewn with dead antelope, the feral apple tree lost to the woods, the farmer lost in their work, slowing becoming less and …

Conserving And Sharing Freshwater In The West, Kelsea Harris-Capuano

Graduate Student Theses, Dissertations, & Professional Papers

There are infinite stories to be told about water in the West. As an essential and finite resource, countries, states, tribal nations and neighbors must figure out how to manage this shared resource, whether for ecosystem sustainability, agricultural, household, or recreational use.

The Flathead Lake Biological Station in Polson, MT is one of the oldest active biological research stations in the United States. One of its former faculty, Dr. Mark Lorang, has been working on Flathead Lake’s erosion problem for over 30 years. As a result of seasonal lake level fluctuations controlled by the dam, erosion over the years has …

Rancher Perspectives On Livelihood Diversification Options In Montana's Blackfoot River Watershed, Sara N. Schmidt

Graduate Student Theses, Dissertations, & Professional Papers

The Blackfoot River watershed in Western Montana, like many rural landscapes across the Intermountain West, has faced a suite of economic, demographic, and social change over the last 40 years. Cattle ranching specifically, a principal land use in the Blackfoot watershed, has faced increasing socioeconomic challenges due to global competition, falling cattle prices, amenity migration, and climate change. Ranch livelihood diversification presents an opportunity to bolster the profitability of ranches in the watershed, while also supporting the well-being of rural communities and conserving the intact, ecologically-significant rangelands upon which ranching depends. The Blackfoot Challenge (BC) is a nonprofit community-based conservation …

The Mountain Home: Spatial And Optimal Foraging Assessments Of Hunter- Gatherer Mountain Landscape Use In The Beartooth Mountains, Montana, Scott William Dersam

Graduate Student Theses, Dissertations, & Professional Papers

Patterns of cultural mountain adaptations are recorded throughout the Western Cordillera of North America. They demonstrate prolonged and complex uses of these ecosystems over the Holocene. What behaviors or circumstances in the Early Holocene transitioned mountains from inhospitable to reliable territory? This research addresses this question in the Beartooth Mountains of the Greater Yellowstone Ecosystem (GYE).

Foraging behaviors dictate hunter-gatherer mobility and habitation patterning. These behaviors are reflected in the spatial arrangement of archaeological activity areas and lithic tools in mountainous ecosystems. Energy-demanding ecosystems, require foraging behaviors and habitation strategies that conserve energy. I assert that to understand the emergence …

Functional And Pharmacological Characterization Of Glun3-Containing Nmda Receptors, Nirvan Rouzbeh

Graduate Student Theses, Dissertations, & Professional Papers

N-methyl-D-aspartate (NMDA) receptors are a member of ionotropic glutamate receptors that mediate excitatory neurotransmission across the mammalian central nervous system. NMDA receptors are organized into heterotetrameric assemblies containing two obligatory GluN1 subunits and two GluN2A-D, or GluN3A-B subunits. GluN1 and GluN3A-B bind glycine or D-serine, whereas GluN2A-D bind glutamate. The structural, functional, and pharmacological investigation of GluN1/3 NMDA receptors has been greatly hindered by their unconventional properties such as impermeability to Ca2+, insensitivity to the voltage-dependent block by Mg2+, and robust desensitization upon binding their endogenous agonist glycine. Another major barrier in GluN3 research has been the lack of selective …

Body Temperature And Reproductive Effort In A Long-Lived Tropical Songbird, Holly R. Jackson

Graduate Student Theses, Dissertations, & Professional Papers

Heat production relative to dissipation rates have constrained reproductive effort and reduced fitness in short-lived, temperate birds. To determine whether heat constrains reproductive effort in long-lived taxa with low reproductive effort, we experimentally clipped plumage from tropical Gray-throated babblers (Stachyris nigriceps) to increase heat dissipation rates. Contrary to findings in short-lived species, we found no strong evidence of heat dissipation constraints on reproductive effort in our mid-elevation study. Clipped adults did not increase feeding rates compared to controls, but clipped females did spend more time incubating and brooding eggs and young. Increased time in the nest may reduce increased heat …

Wilderness As An Aesthetic Concept, Nathan A. Pagel

Graduate Student Theses, Dissertations, & Professional Papers

In this paper I argue that many of the philosophical problems with the concept of wilderness can be mitigated by thinking of wilderness as an aesthetic concept rather than as a real feature of the world or a special metaphysical category. In Section 1, I respond to arguments for wilderness no longer being extant in the world as well as being conceptually contradictory by examining the ways in which people continue to experience wilderness in spite of these challenges. To resolve this tension, I offer an account of wilderness as an aesthetic concept, that is, as something that cannot be …

Feeling The Burn: Assessing The Effects Of Fire Severity On Elk Nutritional Resources And Resource Selection, Lauren Ashley Snobl

Graduate Student Theses, Dissertations, & Professional Papers

Wildfires in the western United States have been increasing in size and severity over the last several decades, and climate forecasts suggest a continued increase in wildfire activity in the future. Large-scale wildfires often burn in a mosaic-like pattern of varying fire severity creating a heterogenous landscape with concomitant effects on vegetation communities. By changing vegetative structure, fire severity potentially alters the availability and distribution of key resources for wildlife such as food and cover, ultimately influencing habitat use. However, the impact of fire on wildlife has frequently been treated as binary (i.e., burned vs unburned), and a deeper understanding …

Epistemoligical Uncertainty And Political Ideology: Exploring The Relationship Between Temporal Threats And Ideology, James Donald Mcfarland

Graduate Student Theses, Dissertations, & Professional Papers

Research in motivation suggests that individuals are highly attuned to perceived risk and danger and tend to form groups (physical and ideological) on the basis of the threats they collectively share with like-minded others. These initial stages of threat detection and evaluation are often found to occur through subtle environmental cues. This study examined the relationship between feelings of uneasiness and political ideology through the framing of temporal cues (e.g., past- vs. future-tense). Participants (n = 181) were recruited through Amazon’s Mechanical Turk (MTurk) and were randomly assigned a short vignette about a relatable life experience (e.g., purchasing a car). …