Digital Commons Network^™

Modified Tlr7/8 Adjuvanted Liposomes For Improved Intranasal Delivery Of Subunit Vaccines, Elizabeth Brander Lorentz

Graduate Student Theses, Dissertations, & Professional Papers

Intranasal vaccines hold enormous potential for improving vaccine efficacy against respiratory pathogens by activating the mucosal immune system. The urgency for novel approaches in vaccine development is amplified by the recent COVID-19 pandemic. Most intranasal vaccines that were developed are either live-attenuated or inactivated vaccine candidates that demonstrated poor safety profiles. Protein-based subunit vaccines represent a safe and cost-effective alternative, but they are often weak immunogenically. TLR7/8 adjuvants such as INI-4001 have shown promise for enhancing the humoral and cellular immunogenicity of protein-based subunit vaccines. It is theorized that poor immunogenicity of intranasal protein subunit vaccines is due to low …

Causes Of Low Leaf-Shredding Insect Diversity In Neotropical Streams, Pavel Ernesto Garcia Soto

Graduate Student Theses, Dissertations, & Professional Papers

Biological diversity peaks in tropical regions followed by a steady decline towards the poles. It is a pattern in γ-diversity that sometimes explains latitudinal patterns in local assemblages (α) and their species turnover (β), which suggests that similar ecological mechanisms might be operating at different latitudes. However, not all taxa have more species towards the equator. Aquatic shredder insect richness peaks in temperate streams relative to tropical streams, where local aquatic shredder insect assemblages have low α-diversity. Three mechanisms have been proposed as factors structuring aquatic shredder insect assemblages in tropical streams, leading to shredder assemblages with low α-diversity, 1) …

Evaluating Nurse Engagement With And Opportunities For Human Papillomavirus (Hpv) Vaccine Promotion In Montana, Juthika Jayendra Thaker

Graduate Student Theses, Dissertations, & Professional Papers

Purpose: Parental vaccine hesitancy is a known driver of sub-optimal vaccine uptake in the United States.1 Even though a study analyzing parental responses in National Immunization Survey-Teen found that HPV vaccine hesitancy has slightly declined (69% in 2010 v/s 63% in 2019) over the years, only about 52.6% of adolescents in Montana had received all the required doses of the HPV vaccine series in 2021.2,3 Nurses are at the forefront of healthcare provision and possess a unique ability to influence parental vaccine decisions.5 By listening to and addressing parents' concerns about immunizations, nurses can dispel misinformation and help change parents' …

“Frozen”: Tonic Immobility And Posttraumatic Outcomes Among Survivors Of Sexual Assault, Nora Grace Halverson

Graduate Student Theses, Dissertations, & Professional Papers

Tonic immobility (TI), a temporary, involuntary state of paralysis marked by motor inhibition and vocal suppression, has been a well-documented occurrence among animals. Humans also experience TI, which has previously been referred to as trauma- or rape-induced paralysis and freezing. TI among survivors of sexual assault has been associated with greater PTSD and increased risk of depression. Existing studies have not adequately explored additional posttraumatic outcomes of TI beyond PTSD diagnostic criteria, nor has prior research sufficiently tested the mechanistic relationship between TI and PTSD. The objective of the current study was to integrate and expand upon prior research regarding …

Predicting Gene Flow Corridors And Wildlife Health Using Landscape Genomics And Non-Invasive Metagenomic Monitoring: Investigation Of Elk (Cervus Canadensis) In The Greater Yellowstone Ecosystem, Samuel B. Pannoni

Graduate Student Theses, Dissertations, & Professional Papers

This work develops and applies novel methods for metagenomic and landscape genomic (LSG) investigation of wildlife populations, specifically in Rocky Mountain elk (Cervus canadensis) in the Greater Yellowstone Ecosystem (GYE). Host fecal microbiomes facilitate and reflect interactions between the host and its environment. We conducted metagenomic surveys and developed a machinelearning classifier for microbiomes that provided new insights into elk-microbiome biogeography, and the impact of fecal microbiomes on host health and the ecology of elk-wolf (Canis lupus) predation. In the microbiome study, we found strong evidence for multiple management-relevant factors structuring elk microbiomes including body fat percentage, sex, age, and …

Linking Life History And Population Dynamics Of An Ice-Associated Seabird, The Kittlitz’S Murrelet (Brachyramphus Brevirostris), Michelle Lynn Kissling

Graduate Student Theses, Dissertations, & Professional Papers

Mechanistic understanding of population dynamics requires linking population change and demographic processes. However, for some species, accurate estimation of population parameters can be difficult owing to their life histories, resulting in reduced or biased inference. Mobile species that are not territorial or use dynamic habitats are susceptible to estimation problems arising from variable exposure to sampling, or temporary emigration, and common approaches to account for it like robust design are not feasible. The outcome is a population mismatch whereby the statistical population, or what was sampled, is not aligned with the biological population, which is what we want to know …

Impacts Of Conifer Removal On Songbird Density, Reproductive Output, And Habitat Selection, Elise Catherine Zarri

Graduate Student Theses, Dissertations, & Professional Papers

Restoration of anthropogenically altered habitats is vital to protect biodiversity and prevent species extinction as landscapes are increasingly altered, degraded, and lost. However, restoration is often done without a full understanding of its impact on biological communities. Lack of knowledge can lead to unintended negative consequences for some species restoration was intended to benefit. Woody plant encroachment is a form of habitat alteration occurring in grasslands and shrublands around the globe, driven by anthropogenic shifts in disturbance regimes. One example is conifer expansion into historically widespread sagebrush communities. Sagebrush restoration through conifer removal has become a common strategy to aid …

Sagebrush Steppe Songbird Conservation: Inferences Using Remote Sensing Data And A Novel Reproduction Model, Kaitlyn Marie Reintsma

Graduate Student Theses, Dissertations, & Professional Papers

Anthropogenic land cover change continues to degrade and fragment wildlife habitats, threatening biodiversity at local, regional, and global scales. Songbird populations are sensitive to broad habitat changes and therefore can be used to assess and prioritize habitat for biodiversity. For example, biodiversity losses in the arid grass and sagebrush of western North America (Artemisia spp.; hereafter “sagebrush steppe”) are reflected in the rapid decline of songbird populations. However, the influence of the principal land use, livestock grazing, on songbird populations, and by inference biodiversity, is unclear. In this dissertation, I investigated sagebrush steppe songbird conservation in a grazed landscape by …

The Biome Within: Conception And Change In The Paradise Valley, Austin Kirchhoff

Graduate Student Theses, Dissertations, & Professional Papers

The Biome Within is an essay collection that meditates on change. Born and raised in the Paradise Valley of southwest Montana, Austin recounts stories from her childhood, painting a picture of rural life in the Valley that contrasts with its modern-day incarnation as a luxury get-away and millionaire’s playground. Even as Austin pines for a time and a place that no longer exists, embodying the nostalgia that she identifies in the Valley’s transplants, the reader comes to understand that the author – and her family’s way of making a living – are culpable in creating the changes that she now …

The Birds And The Trees: Quantifying The Drivers Of Whitebark Pine Decline And Clark's Nutcracker Habitat Use In Glacier National Park, Vladimir Kovalenko

Graduate Student Theses, Dissertations, & Professional Papers

Whitebark pine (Pinus albicaulis), recently listed as threatened under the Endangered Species Act, is in steep decline in Glacier National Park, Montana, USA due to the non-native pathogen Cronartium ribicola, causal agent of the fatal disease white pine blister rust. A sample of the park’s population suggests that approximately 70 percent of whitebark pines have died, while 65 percent of the remaining trees are infected. Using landscape and climate variables, we show how geographic location, elevation, aspect, solar radiation, relative humidity, and snowpack interact with tree diameter to affect mortality, disease incidence, cone production, and regeneration. We also examine how …

Strange Creature, Dagny Walton

Graduate Student Theses, Dissertations, & Professional Papers

Strange Creature is an exploration and renovation of the myth of the American West. I extract elements from the known and recognizable myth of the West and create my own rendition, focusing in particular on themes of transformation and violence. Here in this black mirror world, animals speak out loud, cowboys face down a wildland with eyes, and two suns light up the lonely sky. There is no continuous narrative thread, but each piece is a vignette that takes place in a single shared world. This world is at once familiar and completely alien. I intend to surprise the viewer …

Making Mochi!, Shannon 'Owo' Crystal Webb

Graduate Student Theses, Dissertations, & Professional Papers

Making mochi (rice cakes) is a metaphor for making a way of life for myself through spiritual and cultural practice. I navigate the complexities of cultural mixing and identity as someone who is half Korean and half white. Rather than one or the other, I have always felt mixed, so my path to understanding my place in the world is also mixed. On one level, I am honoring my heritage by referencing Korean customs, folktales, and mythology. On another, I address how my needs are based on my current state, which includes my location, pop culture, and society at large. …

From The Green Room: Notes On A Saltwater Girlhood In The Borderlands, Izabela Garcia-Arce

Graduate Student Theses, Dissertations, & Professional Papers

In this debut book, Bela García-Arce explores what it means to reconnect with saltwater roots in tiny coastal towns across Baja California through a collection of personal essays. She starts by telling the story of her foundation—about growing up in San Diego and falling in love with the ocean, about what it was like to be a young Mexican girl with a family with no interest in the water. She leans heavily on humor to deliver playful jabs at the toxic masculine beach culture while also revealing how she played a role in combatting it. Between exploration of toxic sludge …

The Reproductive Consequences Of Low Energy Availability In Females, Courtney Couture

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

Motown Movie Magic: Respectability, Gender, And Authenticity In Crossover Films, 1972-1989, Nicholas Andrew Ambs

Graduate Student Theses, Dissertations, & Professional Papers

At the start of the 1970s, Berry Gordy, Jr., moved Motown Industries to Los Angeles to expand into the film and television industries. Just as in the music industry, Gordy utilized respectability politics to navigate a segregated market to appeal to a wider audience. As rhetoric around notions of respectability changed perspectives on the Black experience, Gordy’s business practices represented a traditional tactic for uplift ideology that he sought to demonstrate in his film. In the context of national changes and industrial trends, Gordy balanced building credibility, establishing a profitable studio, and creating a positive image throughout the 70s and …

Responses Of Elk To Mangement Actions And Hunter Access In The Northern Sapphire Mountains, Montana, Usa, Peter O. Mumford

Graduate Student Theses, Dissertations, & Professional Papers

Chapter 1

Regulation of rifle hunting of adult female elk is the main tool wildlife managers use to manage the abundance of elk (Cervus canadensis) in the western United States. However, elk are increasingly located in areas where hunting access is restricted during rifle-hunting season, decreasing the ability of managers to reduce elk abundance through hunter harvest. Management actions that restricted hunter access in a 26 km2 area and closed 62 kms of predominantly 2-wheel motorized routes occurred in the northern Sapphire Mountains, Montana, USA. We collected data on elk locations before and two years after the management actions and …

A Dual-Function Filamentous Pf Bacteriophage Protein Modulates Pseudomonas Aeruginosa Virulence Potential, Caleb Michael Schwartzkopf

Graduate Student Theses, Dissertations, & Professional Papers

Pseudomonas aeruginosa is an opportunistic pathogen that often plagues hospitals. More than half of P. aeruginosa isolates are infected by temperate Pf bacteriophage. Pf virions protect bacteria from antibiotics, promote biofilm formation, and modulate animal immune responses in ways that promote chronic infection. These virions can be produced without lysing P. aeruginosa, but lysis may occur during superinfection. Superinfection is the process whereby virions of the same or similar phage infect an already infected host. Temperate phages typically encode superinfection exclusion mechanisms. Here we elucidate one such mechanism in Pf phage. We observed that superinfection-surviving P. aeruginosa were transiently resistant …

How Aquatic Insects Mitigate Temperature-Oxygen Challenges Via Behavioral, Morphological, And Physiological Plasticity, Jackson H. Birrell

Graduate Student Theses, Dissertations, & Professional Papers

How do organisms respond to environmental challenges and to environmental change? These questions occupy a central place in ecology and answering them will help us to understand why species live where they do, how organisms are affected by human activities, and, ultimately, how to choose among alternative conservation strategies. These questions are difficult, however, for two reasons. First, environmental challenges often involve multiple, interacting stressors. Second, individual responses can be modified by behavioral, morphological, and physiological plasticity. My dissertation investigates how interactions between temperature and oxygen influence the performance and survival of aquatic insects and how plasticity allows individuals to …

The Effect Of Place Attachment And Leisure Identity On Stewardship Participation In The Rattlesnake National Recreation Area And Wilderness, Elena Rene Thomas

Graduate Student Theses, Dissertations, & Professional Papers

As outdoor recreation participation increases in the U.S., and many communities in the American West are experiencing rising amenity migration, park and protected area managers face significant challenges to balance both providing for visitors’ desired experiences and protecting the natural resources on which these experiences depend. Confronted with declining management capacity, agencies are increasingly looking to engage both newer and established recreationists in stewardship behaviors to help maintain these areas and improve human-environment relationships. One barrier to community involvement in stewardship efforts is the need to better understand the connection between recreationists’ antecedent conditions and their motivations to steward. Past …

Municipal Musk Oxen, Gentry Hale

Graduate Student Theses, Dissertations, & Professional Papers

You’d be hard-pressed to find a town with more sled dogs and mushers per capita than Nome, the finish line of the 938-mile Iditarod sled dog race.

It is also home to a growing herd of musk oxen, massive ungulates that Congress reintroduced to the Seward Peninsula in 1970. These icons of the far north have changed little over their 1.8 million years of existence and more closely related to sheep and goats than actual oxen.

While musk oxen pose a risk to dogs and people, they are also a major tourist attraction that helps inject much needed outside cash …

Tlr4 Adjuvanted Vaccine Against Pseudomonas Aeruginosa Virulence Factor, Pf Bacteriophage, Induces Increased Immunoglobulin Response And Highly Mutated Antigen-Specific B-Cell Receptors, Valery Cristina Roman-Cruz

Graduate Student Theses, Dissertations, & Professional Papers

Pseudomonas aeruginosa is listed as a priority 1 pathogen by the World Health Organization. Although this Gram-negative bacterium is ubiquitous and can naturally be found in the environment, it is opportunistic. P. aeruginosa has a wide range of host immune evasion strategies; one such strategy is the establishment of biofilms. Pf bacteriophage, a P. aeruginosa virulence factor, can strengthen the integrity of biofilms and can also misdirect the host immune response from an appropriate anti-bacterial response to an anti-viral response. Previous studies have established that antibodies against Pf bacteriophage can enhance clearance of P. aeruginosa infections. Next generation adjuvants such …

A Biophysical Approach To Modeling Elevational Range Shifts In Colorado Mammal Communities, Ryan T. Mahar

Graduate Student Theses, Dissertations, & Professional Papers

Species geographic ranges are shifting in the face of contemporary climate warming, and documenting range shifts is crucial to our understanding of the underlying drivers mediating movement in geographic range limits. Studies on elevational range shifts with climate change are beginning to accrue within the literature, though observed shifts are idiosyncratic and difficult to predict. Some species may respond to warming temperatures by shifting their range limits upslope, where temperatures are cooler owing to the adiabatic lapse rate. However, species may also respond to warming temperatures in an elevation-dependent manner: if changes in snow depth expose overwintering organisms to colder …

Life After Ice: Ecology Of Mountain Lakes Across Glacial And Elevation Gradients, Joseph William Vanderwall

Graduate Student Theses, Dissertations, & Professional Papers

Conceptual models of primary succession in lakes often do not match with empirical data. New lakes arising in mountain regions due to the loss of alpine glaciers offer an opportunity to study newly forming lakes. In addition to the loss of alpine glaciers, mountain lakes are currently experiencing rapid changes, including increased vegetation cover, variable snowpack, and increased nutrient deposition. In this dissertation, I used glacial and elevation gradients to investigate the ecology and biogeochemistry of early lakes and how they may change through time. The first chapter details the glacial gradient and shows that early lakes dominated by glacial …

Comedy, Camaraderie, And Conflict: Using Humor To Defuse Disputes Among Friends, Sheena A. Bringa

Graduate Student Theses, Dissertations, & Professional Papers

This study sought to examine the role humor plays in defusing conflict between friends from an evolutionary perspective. Although a vast amount of research exists on humor, friendship, and conflict, no single study connects all three of these concepts together. This study attempted to fill this gap by examining how different humor styles used between friends in times of conflict relate to friendship satisfaction and life satisfaction. Specifically, the hypotheses predicted that friends who use affiliative humor to deescalate conflict are more inclined to report higher relational satisfaction and improved individual well-being than friends who use maladaptive humor to deescalate …

In The Weeds, Bianca J. Jensen

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

Governing Human-Elephant Conflict: A Critical Institutional Approach To Decolonizing Conservation In The Chobe Enclave Of Botswana, Jake Rayapati

Graduate Student Theses, Dissertations, & Professional Papers

Within the largest population of African savanna elephants (Loxodonta africana) on the continent, Botswana’s Chobe Enclave is a group of agricultural communities bounded by protected areas and an international border. Human-elephant conflict (HEC) is a major concern for both Enclave residents and conservation practitioners. This research asked how institutional arrangements shape the governance of HEC in the Chobe Enclave. Previous research has shown that technical solutions to HEC must be complemented by integrating governance across local, national, and international levels to make elephant conservation and management more inclusive of people who live alongside elephants. This research employed a …

Montana's Local Exotic Pet Trade, Alexa Bene Hansen

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

Three-Dimensional Visualization And Mechanical Assessment Of The Human Running Gait, John A. Leske Ii

Graduate Student Theses, Dissertations, & Professional Papers

This thesis had two goals; 1) the development of an anatomically accurate visualization tool capable of illustrating high-speed 3D kinematic data, and 2) to rigorously evaluate the accuracy and capacity of the visualization tool by measuring the joint moments, net extensor muscle forces, and effective mechanical advantage in a group of sprinters running across the range of human speeds. Our visualization tool successfully integrates these complexities, pinpointing a location within 1.3 mm in a volume of 0.87 m x 2.00 m x 1.98 m, and accurately reproduces the observed movement trajectories of the subject's anatomical landmarks. Our approach was successful …

Greater Sage-Grouse In A Grazed Landscape: Habitat Selection, Demographic Rates, And Population Trends In Central Montana, Jennifer Evans Helm

Graduate Student Theses, Dissertations, & Professional Papers

Greater sage-grouse (Centrocercus urophasianus) abundance has declined across the species’ range due to habitat loss, degradation, and fragmentation. To address this decline, information is needed to guide habitat conservation priorities and population management efforts. This includes information about patterns of habitat selection at multiple spatial scales, habitat and land use variables that affect demographic rates, and population trend estimates. We collected ten years of data (2011-2020) on sage-grouse demographic rates and habitat selection, as well as on vegetation and livestock grazing patterns, to address these topics. We were specifically interested in the effects of a rotational grazing system implemented through …

Understanding Identity, Power, And Use Of Space Over Time Within Housepit 54, Bridge River Site (K’Etxelkná’Z), British Columbia, Ashley Elizabeth Hampton

Graduate Student Theses, Dissertations, & Professional Papers

This research examines cultural change and continuity as embodied within a singular multi-generational housepit (Housepit 54) located within the Bridge River site (K’etxelkná’z) in the Mid-Fraser Canyon, British Columbia, Canada. Previous research has highlighted the distinctive differences between Bridge River 2 and 3 time periods wherein the village was faced with dramatic population growth and climate change. These pressures crafted a Malthusian ceiling-type event which corresponded with the emergence of persistent institutionalized inequality. This research aims to illuminate issues of gender, kinship, social identity, and household social relationships in order to highlight how macro-scale cultural change is evinced in micro-scale …