Digital Commons Network^™

Structural Insights Into Peptidoglycan Glycosidase Etga Binding To The Inner Rod Protein Esci Of The Type Iii Secretion System Via A Designed Esci-Etga Fusion Protein, Jacob Boorman, Focco Van Den Akker

Faculty Scholarship

Bacteria express lytic enzymes such as glycosidases, which have potentially self-destructive peptidoglycan (PG)-degrading activity and, therefore, require careful regulation in bacteria. The PG glycosidase EtgA is regulated by localization to the assembling type III secretion system (T3SS), generating a hole in the PG layer for the T3SS to reach the outer membrane. The EtgA localization was found to be mediated via EtgA interacting with the T3SS inner rod protein EscI. To gain structural insights into the EtgA recognition of EscI, we determined the 2.01 Å resolution structure of an EscI (51–87)-linker-EtgA fusion protein designed based on AlphaFold2 predictions. The structure …

A General Materials Data Science Framework For Quantitative 2d Analysis Of Particle Growth From Image Sequences, Sameera Nalin Venkat, Thomas G. Ciardi, Mingjian Lu, Preston C. Deleo, Jube Augustino, Adam Goodman, Jayvic Cristian Jimenez, Anirban Mondal [email protected], Frank Ernst, Yinghui Wu, Roger H. French, Laura S. Bruckman

Faculty Scholarship

Phase transformations are a challenging problem in materials science, which lead to changes in properties and may impact performance of material systems in various applications. We introduce a general framework for the analysis of particle growth kinetics by utilizing concepts from machine learning and graph theory. As a model system, we use image sequences of atomic force microscopy showing the crystallization of an amorphous fluoroelastomer film. To identify crystalline particles in an amorphous matrix and track the temporal evolution of the particle dispersion, we have developed quantitative methods of 2D analysis. 700 image sequences were analyzed using a neural network …

Effects Of Micromachining On Anti-Oxidant Elution From A Mechanically-Adaptive Polymer, Natalie N. Mueller, Youjoung Kim, Mali Ya Mungu Ocoko, Peter Dernelle, Ishani Kale, Simran Patwa, Anna Clarissa Hermoso, Deeksha Chirra, Jeffrey R. Capadona, Allison E. Hess-Dunning

Faculty Scholarship

Intracortical microelectrodes (IMEs) can be used to restore motor and sensory function as a part of brain–computer interfaces in individuals with neuromusculoskeletal disorders. However, the neuroinflammatory response to IMEs can result in their premature failure, leading to reduced therapeutic efficacy. Mechanically-adaptive, resveratrol-eluting (MARE) neural probes target two mechanisms believed to contribute to the neuroinflammatory response by reducing the mechanical mismatch between the brain tissue and device, as well as locally delivering an antioxidant therapeutic. To create the mechanically-adaptive substrate, a dispersion, casting, and evaporation method is used, followed by a microfabrication process to integrate functional recording electrodes on the material. …

Factors Influencing Music Therapists’ Retention Of Clinical Hours With Autistic Clients Over Telehealth During The Covid-19 Pandemic, Nicole M. Richard Williams

Faculty Scholarship

The COVID-19 pandemic influenced music therapists to migrate services to online platforms, though some lost clinical hours during the pandemic when telehealth was not a viable option. This survey study aimed to ascertain factors that helped music-based therapists to continue serving autistic clients over telehealth during the pandemic. We surveyed 193 accredited music therapists located mainly in Canada and the United States. In addition to gathering data on general perceptions of telehealth music therapy and Neurologic Music Therapy® (NMT), one-way ANOVAs were applied to determine differences in percent-change loss of clinical hours for music therapists: 1) working in different employment …

In Vivo Characterization Of Intracortical Probes With Focused Ion Beam-Etched Nanopatterned Topographies, Jonathan L. Duncan, Jaime J. Wang, Gabriele Glusauskas, Yue Gao, George F. Hoeferlin, Allison E. Hess-Dunning, Jeffrey R. Capadona

Faculty Scholarship

(1) Background: Intracortical microelectrodes (IMEs) are an important part of interfacing with the central nervous system (CNS) and recording neural signals. However, recording electrodes have shown a characteristic steady decline in recording performance owing to chronic neuroinflammation. The topography of implanted devices has been explored to mimic the nanoscale three-dimensional architecture of the extracellular matrix. Our previous work used histology to study the implant sites of non-recording probes and showed that a nanoscale topography at the probe surface mitigated the neuroinflammatory response compared to probes with smooth surfaces. Here, we hypothesized that the improvement in the neuroinflammatory response for probes …

Integrating Multiscale Geospatial Analysis For Monitoring Crop Growth, Nutrient Distribution, And Hydrological Dynamics In Large-Scale Agricultural Systems, Olatunde D. Akanbi, Deepa C. Bhuvanagiri, Erika I. Barcelos, Arafath Nihar, Brian Gonzalez Hernandez, Jeffrey M. Yarus, Roger H. French

Faculty Scholarship

Monitoring crop growth, soil conditions, and hydrological dynamics are imperative for sustainable agriculture and reduced environmental impacts. This interdisciplinary study integrates remote sensing, digital soil mapping, and hydrological data to elucidate intricate connections between these factors in the state of Ohio, USA. Advanced spatiotemporal analysis techniques were applied to key datasets, including the MODIS sensor satellite imagery, USDA crop data, soil datasets, Aster GDEM, and USGS stream gauge measurements. Vegetation indices derived from MODIS characterized crop-specific phenology and productivity patterns. Exploratory spatial data analysis show relationships of vegetation dynamics and soil properties, uncovering links between plant vigor, edaphic fertility, and …

Integrating Multiscale Geospatial Analysis For Monitoring Crop Growth, Nutrient Distribution, And Hydrological Dynamics In Large-Scale Agricultural Systems, Olatunde D. Akanbi, Deepa C. Bhuvanagiri, Erika I. Barcelos, Arafath Nihar, Brian Gonzalez Hernandez, Jeffrey M. Yarus, Roger H. French

Faculty Scholarship

Monitoring crop growth, soil conditions, and hydrological dynamics are imperative for sustainable agriculture and reduced environmental impacts. This interdisciplinary study integrates remote sensing, digital soil mapping, and hydrological data to elucidate intricate connections between these factors in the state of Ohio, USA. Advanced spatiotemporal analysis techniques were applied to key datasets, including the MODIS sensor satellite imagery, USDA crop data, soil datasets, Aster GDEM, and USGS stream gauge measurements. Vegetation indices derived from MODIS characterized crop-specific phenology and productivity patterns. Exploratory spatial data analysis show relationships of vegetation dynamics and soil properties, uncovering links between plant vigor, edaphic fertility, and …

Using Spatio-Temporal Graph Neural Networks To Estimate Fleet-Wide Photovoltaic Performance Degradation Patterns, Yangxin Fan, Raymond Wieser, Xuanji Yu, Yinghui Wu, Laura S. Bruckman, Roger H. French

Faculty Scholarship

Accurate estimation of photovoltaic (PV) system performance is crucial for determining its feasibility as a power generation technology and financial asset. PV-based energy solutions offer a viable alternative to traditional energy resources due to their superior Levelized Cost of Energy (LCOE). A significant challenge in assessing the LCOE of PV systems lies in understanding the Performance Loss Rate (PLR) for large fleets of PV systems. Estimating the PLR of PV systems becomes increasingly important in the rapidly growing PV industry. Precise PLR estimation benefits PV users by providing real-time monitoring of PV module performance, while explainable PLR estimation assists PV …

Using Spatio-Temporal Graph Neural Networks To Estimate Fleet-Wide Photovoltaic Performance Degradation Patterns, Yangxin Fan, Raymond Wieser, Xuanji Yu, Yinghui Wu, Laura S. Bruckman, Roger H. French

Faculty Scholarship

Accurate estimation of photovoltaic (PV) system performance is crucial for determining its feasibility as a power generation technology and financial asset. PV-based energy solutions offer a viable alternative to traditional energy resources due to their superior Levelized Cost of Energy (LCOE). A significant challenge in assessing the LCOE of PV systems lies in understanding the Performance Loss Rate (PLR) for large fleets of PV systems. Estimating the PLR of PV systems becomes increasingly important in the rapidly growing PV industry. Precise PLR estimation benefits PV users by providing real-time monitoring of PV module performance, while explainable PLR estimation assists PV …

Definitions Of Recovery And Reintegration Across The First Year: A Qualitative Study Of Perspectives Of Persons With Spinal Cord Injury And Caregivers, Kim D. Anderson, Anne M. Bryden, Brian Gran, Susan W. Hinze

Faculty Scholarship

Study Design: Longitudinal, qualitative cohort study. Objectives: To understand how people with newly acquired spinal cord injury (PWS) and their support person (SP) define recovery and successful community reintegration (CR) across the first 12 months post-injury (mpi) and their satisfaction with the rate of recovery and reintegration experienced. Setting: Academic and Veterans hospitals in Midwest USA. Methods: In-depth, semi-structured interviews were conducted in two cohorts of PWS and SP during the initial inpatient rehabilitation stay, at 6 mpi, and at 12 mpi. Recordings were transcribed; four authors independently undertook line-by-line coding. The team discussed codes to reach consensus and synthesize …

Real-Time Assessment Of Rodent Engagement Using Aruco Markers: A Scalable And Accessible Approach For Scoring Behavior In A Nose-Poking Go/No-Go Task, Jeffrey R. Capadona

Faculty Scholarship

In the field of behavioral neuroscience, the classification and scoring of animal behavior play pivotal roles in the quantification and interpretation of complex behaviors displayed by animals. Traditional methods have relied on video examination by investigators, which is labor-intensive and susceptible to bias. To address these challenges, research efforts have focused on computational methods and image-processing algorithms for automated behavioral classification. Two primary approaches have emerged: marker-and markerless-based tracking systems. In this study, we showcase the utility of “Augmented Reality University of Cordoba” (ArUco) markers as a marker-based tracking approach for assessing rat engagement during a nose-poking go/no-go behavioral task. …

Flow Cytometry Of Dnmt1 As A Biomarker Of Hypomethylating Therapies, Philip G. Woost, Basem M. William, Brenda W. Cooper, Masumi Ueda Oshima, Folashade Otegbeye, Marcos J. De Lima, David Wald, Reda Z. Mahfouz, Yogen Saunthararajah, Tammy Stefan, James W. Jacobberger

Faculty Scholarship

The 5-azacytidine (AZA) and decitabine (DEC) are noncytotoxic, differentiation-inducing therapies approved for treatment of myelodysplastic syndrome, acute myeloid leukemias (AML), and under evaluation as maintenance therapy for AML postallogeneic hematopoietic stem cell transplant and to treat hemoglobinapathies. Malignant cell cytoreduction is thought to occur by S-phase specific depletion of the key epigenetic regulator, DNA methyltransferase 1 (DNMT1) that, in the case of cancers, thereby releases terminal-differentiation programs. DNMT1-targeting can also elevate expression of immune function genes (HLA-DR, MICA, MICB) to stimulate graft versus leukemia effects. In vivo, there is a large inter-individual variability in DEC and 5-AZA activity because of …

Dose Tapering And Discontinuation Of Biologic Dmards In Axial Spondyloarthritis: A Narrative Review (2023 Spartan Annual Meeting Proceedings), Haseeb Chaudhary, Ansaam Daoud, Marina Magrey

Faculty Scholarship

Purpose of Review: Limited data is available for tapering or discontinuation of biologic therapy in patients with axSpA who are in disease remission. The current review concentrates on published studies regarding dose tapering or withdrawal of biologics in axSpA. Recent Findings: Recent evidence in light of randomized controlled trials suggests that tapering of b-DMARDs is a feasible strategy to maintain remission or low disease activity in axSpA patients. TNF inhibitors were the studied biologics in most of these trials. The disease flare rates were comparable to those maintained on standard dose in most of these studies, although with variable tapering …

Composition-Property Relationships Of Choline Based Eutectic Solvents: Impact Of The Hydrogen Bond Donor And Co2 Saturation, Ruth Dikki, Vaishali Khokhar, Muhammad Zeeshan, Oguz Kagan Coskun, Burcu Gurkan

Faculty Scholarship

Eutectic solvents are tunable for targeted applications through the functional groups in their hydrogen bond acceptor (HBA) and hydrogen bond donor (HBD) components, as well as the HBA : HBD composition ratio. This study examines the properties of choline-based eutectics containing imidazole, phenol, pyrrole-2-carbonitrile, and 1,2,4-triazole HBAs, and ethylene glycol, 1,2-propylene glycol, and ethanolamine HBDs. The viscosity, conductivity, degree of hydrogen bonding, thermal stability, and solvatochromic properties are examined as a function of HBA, HBD, and the composition. These studies revealed a predominant dependence of physical properties on the HBD and determined that the strong hydrogen bonding in phenol and …

Intracellular Neural Control Of An Active Feeding Structure In Aplysia Using A Carbon Fiber Electrode Array, Yu Huan, Benjamin N. Tibbetts, Hillel J. Chiel

Faculty Scholarship

Background: To study neural control of behavior, intracellular recording and stimulation of many neurons in freely moving animals would be ideal. However, current technologies limit the number of neurons that can be monitored and manipulated. A new technology has become available for intracellular recording and stimulation which we demonstrate in the tractable nervous system of Aplysia. New method: Carbon fiber electrode arrays (whose tips are coated with platinum-iridium) were used with an in vitro feeding preparation to intracellularly record from and to control the activity of multiple neurons during feeding movements. Results: In an in vitro feeding preparation, the carbon …

Nurse Practitioners In Critical Care Transport, David M. Kaniecki, Robert L. Grabowski, David Holloway

Faculty Scholarship

The inclusion of nurse practitioners (NPs) in critical care transport teams has the potential to enhance patient care and improve team operations. NPs can manage complex clinical situations during transport and excel in various roles such as leadership, education, mentoring, research, quality improvement, and clinical expertise. As we navigate the evolving landscape of critical care transport, it is crucial to explore the potential benefits offered by NPs. Their distinct skills and experiences effectively position them to improve patient outcomes, enhance team performance, and contribute to health care's financial sustainability. This article discusses the role of NPs in critical care transport, …

Point-Of-Care Diagnostic Test For Beta-Thalassemia, Ran An, Umut A. Gurkan

Faculty Scholarship

Hemoglobin (Hb) disorders are among the most common monogenic diseases affecting nearly 7% of the world population. Among various Hb disorders, approximately 1.5% of the world population carries β-thalassemia (β-Thal), affecting 40,000 newborns every year. Early screening and a timely diagnosis are essential for β-thalassemia patients for the prevention and management of later clinical complications. However, in Africa, Southern Europe, the Middle East, and Southeast Asia, where β-thalassemia is most prevalent, the diagnosis and screening for β-thalassemia are still challenging due to the cost and logistical burden of laboratory diagnostic tests. Here, we present Gazelle, which is a paper-based microchip …

The Use Of A Behavior Chain Interruption Strategy To Teach Mands For Help With An Adult With Intellectual Disability And Deaf-Blindness, Hannah E. Thompson, Robbie Hanson

Faculty Scholarship

Individuals with deaf-blindness and co-occurring diagnoses, such as intellectual and developmental disabilities, may experience difculty with independence, specifcally with communication. One behavior-analytic procedure that may be useful for increasing independence and teaching communication to this population is the behavior-chain interruption strategy (BCIS). The current study examined the use of the BCIS to teach a 65-year-old deaf-blind participant with severe intellectual disability to use a SadoTech Elderly Monitoring Pager to notify others in the environment when help was needed. The researcher alternated between establishing operation (EO; help needed, items missing, or inoperable) and abolishing operation (AO; help not needed, items present, …

Enhancing Workplace Neuro Health And Productivity: The Synergy Of Wearable Technology With Biophilic And Oxygenation Strategies, Piper Hutson, James Hutson

Faculty Scholarship

In the contemporary workplace, where a staggering 62% of employees reported experiencing burnout in 2023 according to a Medium article, the integration of wearable technology with biophilic and oxygenation strategies emerges as a vital Neurohemal initiative. This approach is particularly relevant for supporting neurodivergent individuals, as well as those recovering from stroke and long-COVID, in the context of return-to-office mandates. The article underscores the significance of research on increased hydrostatic pressure in circulation, particularly its impact on spinal and spinal cord blood flow during water immersion. This insight lays the groundwork for innovations like non-wet water massage devices, which could …

Automating Selective Area Electron Diffraction Phase Identification Using Machine Learning, Nathaniel Tomczak, Jennifer Carter

Faculty Scholarship

Selective area electron diffraction (SAED) patterns can provide valuable insight into the structure of a material. However, the manual identification of collected patterns can be a significant bottleneck in the overall phase classification workflow. In this work, we utilize the recent advances in computer vision and machine learning (ML) to automate the indexing of SAED patterns. The performance of six different ML algorithms is demonstrated using metallic plutonium-zirconium alloys. The most successful approach trained a neural network (NN) to make a classification of the phase and zone axis, and then utilized a second NN to synthesize multiple independent predictions of …

Fostering Empathetic Career Development Through Non-Violent Communication Principles, Aysegul Keskin Zeren

Faculty Scholarship

Career services professionals can embody compassion and essential dialogue skills by embracing Nonviolent Communication (NVC) principles. This framework can aid career services professionals in serving as powerful role models for students entering the job market. Students will feel empowered to navigate the professional world with increased emotional intelligence. Influential leaders like Satya Nadella, the CEO of Microsoft, recognized the significance of NVC. When he became the CEO of Microsoft in 2014, Nadella decided to use the NVC framework to change the company culture (Abadi, 2018).

Time To Blood Pressure Control And Predictors Among Patients Receiving Integrated Treatment For Hypertension And Hiv Based On An Adapted Who Hearts Implementation Strategy At A Large Urban Hiv Clinic In Uganda, Willington Amutuhaire, Lewis S. Musoke, George A. Yendewa, Lewis S. Musoke, George A. Yendewa

Faculty Scholarship

In this cohort study, we determined time to blood pressure (BP) control and its predictors among hypertensive PLHIV enrolled in integrated hypertension-HIV care based on the World Health Organization (WHO) HEARTS strategy at Mulago Immunosuppression Clinic in Uganda. From August 2019 to March 2020, we enrolled hypertensive PLHIV aged ≥18 years and initiated Amlodipine 5 mg mono-therapy for BP (140–159)/(90–99) mmHg or Amlodipine 5 mg/Valsartan 80 mg duo-therapy for BP ≥ 160/90 mmHg. Patients were followed with a treatment escalation plan until BP control, defined as BP < 140/90 mmHg. We used Cox proportional hazards models to identify predictors of time to BP control. Of 877 PLHIV enrolled (mean age 50.4 years, 62.1% female), 30% received mono-therapy and 70% received duo-therapy. In the monotherapy group, 66%, 88% and 96% attained BP control in the first, second and third months, respectively. For patients on duo-therapy, 56%, 83%, 88% and 90% achieved BP control in the first, second, third, and fourth months, respectively. In adjusted Cox proportional hazard analysis, higher systolic BP (aHR 0.995, 95% CI 0.989-0.999) and baseline ART tenofovir/lamivudine/efavirenz (aHR 0.764, 95% CI 0.637–0.917) were associated with longer time to BP control, while being on ART for >10 years was associated with a shorter time to BP control (aHR 1.456, …

The Physical-Emotional Distinction In Tort, Philip Petrov

Faculty Scholarship

Several legal scholars have recently argued that U.S. tort law’s physical-emotional distinction commits tort to the objectionable position of mind-body dualism, but they have not considered the distinction’s role as an aid to judicial cognition and decision-making. Drawing primarily on the law of negligent infliction of emotional distress, this essay argues that tort’s physical-emotional distinction is not a relic of mind-body dualism but a heuristic that judges have used to structure and simplify the difficult but unavoidable task of drawing lines between legally cognizable and non-cognizable harm. The analysis has at least three normative implications: (1) users of tort’s physical-emotional …

Online Disinhibited Contracts, Wayne R. Barnes

Faculty Scholarship

There have been at least two dominant forces at work in the realm of consumer contracting over the past several decades. One has been the rise and domination of the standard form contract (whereby merchants contract with consumers via the use of standardized, boilerplate terms and conditions that consumers do not read or understand). The second force has been the rise of e-commerce and the purchase of goods and services via websites and other online platforms, and the use of “wrap” formation methodology (whereby merchants obtain consumer assent to the online terms and conditions via the consumer’s informal click, scroll, …

The Wages Of Crying Roe: Some Realism About Dobbs V. Jackson Women’S Health Organization, Neil S. Siegel

Faculty Scholarship

The U.S. Supreme Court’s reasoning in Dobbs v. Jackson Women’s Health Organizationcannot be taken seriously. The Court’s stated rationale for rejecting continued protection of the abortion right—that abortion is not deeply rooted in nineteenth century history and tradition—applies with equal force to other substantive due process rights that the DobbsCourt repeatedly insisted were not under threat even without considering stare decisis, including contraception, sexual intimacy between consenting adults, and same-sex marriage. One should add inter-racial marriage and protection from involuntary sterilization to that list. Rather than applying its draconian version of the “history and tradition” test in a …

Applicable Law And The Civil And Common Law Divide, George A. Bermann

Faculty Scholarship

In examining the relevance of civil law and common law synergies in international arbitration, we often dwell on aspects of arbitral practice and procedure. This should occasion no surprise. Our attention turns to arbitral practice and procedure, for that is what we do, as arbitrator and counsel, and on a daily basis. Leaving aside investor-State arbitration, in which substantive public international law looms large, international arbitration is very much a procedural endeavor.

But it should not be forgotten that the differences between the civil law and the common law manifest themselves not only, and not even primarily, in practice and …

Yes, Trump’S Pacs Really Can Pay His Legal Fees, Richard Briffault

Faculty Scholarship

Campaign finance data released at the end of January 2024 revealed that Save America, a political action committee founded and controlled by former President Donald Trump, spent more than US$50 million in 2023 on legal fees resulting from Trump’s multiple criminal and civil cases.

OpenSecrets, a nonpartisan nonprofit tracking campaign funds, found that other Trump-aligned organizations also paid a combined $10 million in additional legal fees for Trump in 2023.

Though I have spent much of my career as a scholar of campaign finance law, I’m not certain whether that use of campaign donations is legal under federal election law. …

The Crusade For The Holy Grail Of Competitive Balance, Mislav Mataija, Petros C. Mavroidis

Faculty Scholarship

The specificity of sport is a concept acknowledged on both sides of the Atlantic. In the US it is linked to a (limited) exception from antitrust laws. In Europe, the application of the rules on free movement of persons, services, and workers, as well as antitrust, has left some room for it. But whereas the US has gone full steam ahead to regulate competitive balance, Europe has not. In this contribution, inspired by the work of Steve Weatherill and others, we ask the question whether the observed differentiated approach is justified because of the nature of competition on the two …

The Administrative State, Financial Regulation, And The Case For Commissions, Kathryn Judge, Dan Awrey

Faculty Scholarship

Administrative law is under attack, with the Supreme Court reviving, expanding, and creating doctrines that limit the authority and autonomy wielded by regulatory agencies. This anti-administrative turn is particularly alarming for financial regulation, which already faces enormous challenges stemming from the dynamism of modern finance, its growing complexity, and fundamental contestability. Yet that does not mean that defending the current regime is the optimal response. The complexity and dynamism of modern finance also undercut the efficacy of established administrative procedures. And the panoply of financial regulators with unclear and overlapping jurisdictional bounds only adds to the challenge. Both these procedural …

Corpus Linguistics And The Original Public Meaning Of The Sixteenth Amendment, Thomas R. Lee, Lawrence B. Solum, James C. Phillips, Jesse A. Egbert

Faculty Scholarship

Moore v. United States raises the question whether unrealized gains, such as an increase in property value or a stock portfolio, constitute “incomes, from whatever source derived” under the original meaning of the Sixteenth Amendment. Moore is widely viewed as the most important tax case to reach the United States Supreme Court in decades. It is also an opportunity for the Court to refine its theory and method of finding original meaning.

We focus here on the original public meaning of the Sixteenth Amendment—the ordinary, common meaning attributed to its text by the general public in 1913. So far, the …