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Articles 9991 - 10020 of 10789
Full-Text Articles in Entire DC Network
Nutrient Enhanced Coastal Ocean Productivity In The Northern Gulf Of Mexico, William J. Wiseman Jr., Michael J. Dagg, Nancy N. Rabalais, Terry E. Whitledge
Nutrient Enhanced Coastal Ocean Productivity In The Northern Gulf Of Mexico, William J. Wiseman Jr., Michael J. Dagg, Nancy N. Rabalais, Terry E. Whitledge
Faculty Publications
No abstract provided.
Temple And Sacrifice, Roy Gane
The Boundaries Of The Silenced Hmr Domain In Saccharomyces Cerevisiae, David Donze, Christopher R. Adams, Jasper Rine, Rohinton T. Kamakaka
The Boundaries Of The Silenced Hmr Domain In Saccharomyces Cerevisiae, David Donze, Christopher R. Adams, Jasper Rine, Rohinton T. Kamakaka
Faculty Publications
The chromosomes of eukaryotes are organized into structurally and functionally discrete domains that provide a mechanism to compact the DNA as well as delineate independent units of gene activity. It is believed that insulator/boundary elements separate these domains. Here we report the identification and characterization of boundary elements that flank the transcriptionally repressed HMR locus in the yeast Saccharomyces cerevisiae. Deletion of these boundary elements led to the spread of silenced chromatin, whereas the ectopic insertion of these elements between a silencer and a promoter blocked the repressive effects of the silencer on that promoter at HMR and at telomeres. …
Particle Fluxes During Austral Spring And Summer In The Southern Ross Sea, Antarctica, Vernon L. Asper, Walker O. Smith Jr.
Particle Fluxes During Austral Spring And Summer In The Southern Ross Sea, Antarctica, Vernon L. Asper, Walker O. Smith Jr.
Faculty Publications
The flux of particles from the euphotic zone through 200 m was investigated on the Ross Sea continental shelf during two cruises, the first in November-December 1994 and the second in December 1995 and January 1996. An assessment of surface layer phytoplankton biomass and productivity was made simultaneously. Particle flux was measured using floating sediment traps whose collection efficiency was assessed rigorously. Phytoplankton biomass and productivity increased rapidly in November-December, and biomass was maximal in mid-December. Thereafter productivity appeared to decline substantially. Biomass declined as well, but mot as rapidly as productivity. Vertical flux rates were low early in the …
Monte Carlo Study Of The Capacitance Of The Double Layer In A Model Molten Salt, Douglas Henderson, Dezso Boda, Kwong-Yu Chan
Monte Carlo Study Of The Capacitance Of The Double Layer In A Model Molten Salt, Douglas Henderson, Dezso Boda, Kwong-Yu Chan
Faculty Publications
Monte Carlo simulations are reported for charged hard spheres at high density near a charged wall. This system is a simple model for a molten salt double layer. Unfortunately, the reduced temperatures that correspond to experiment are very small. This results in a large Boltzmann factor. As a result, we are unable to obtain meaningful results for such low values and report results only for moderately low values of the reduced temperature. Even so, our results should be a useful benchmark. Further, we are able to give a qualitative answer to an interesting question. We find that at low temperatures …
An Estimate Of The Uptake Of Atmospheric Methyl Bromide By Agricultural Soils, Ruth K. Varner, Patrick M. Crill, Robert W. Talbot, Joanne H. Shorter
An Estimate Of The Uptake Of Atmospheric Methyl Bromide By Agricultural Soils, Ruth K. Varner, Patrick M. Crill, Robert W. Talbot, Joanne H. Shorter
Faculty Publications
Published estimates of removal of atmospheric methyl bromide (CH3Br) by agricultural soils are 2.7 Gg yr−1 (Gg = 109 g) [Shorter et al., 1995] and 65.8 Gg yr−1 [Serça et al., 1998]. The Serça et al. estimate, if correct, would suggest that the current value for total removal of atmospheric CH3Br by all sinks of 206 Gg yr−1 (based on Shorter et al., 1995) would be 30% too low. We have calculated a new rate of global agricultural soil uptake of atmospheric CH3Br from a larger sampling of cultivated soils collected from 40 sites located in the United States, Costa …
Orientation Of Heparin-Binding Sites In Native Vitronectin. Analyses Of Ligand Binding To The Primary Glycosaminoglycan-Binding Site Indicate That Putative Secondary Sites Are Not Functional, Angelia D. Gibson, John A. Lamerdin, Ping Zhuang, Kunnumal Baburaj, Engin H. Serpersu, Cynthia B. Peterson
Orientation Of Heparin-Binding Sites In Native Vitronectin. Analyses Of Ligand Binding To The Primary Glycosaminoglycan-Binding Site Indicate That Putative Secondary Sites Are Not Functional, Angelia D. Gibson, John A. Lamerdin, Ping Zhuang, Kunnumal Baburaj, Engin H. Serpersu, Cynthia B. Peterson
Faculty Publications
A primary heparin-binding site in vitronectin has been localized to a cluster of cationic residues near the C terminus of the protein. More recently, secondary binding sites have been proposed. In order to investigate whether the binding site originally identified on vitronectin functions as an exclusive and independent heparin-hinding domain, solution binding methods have been used in combination with MR and recombinant approaches to evaluate ligand binding to the primary site. Evaluation of the ionic strength dependence of heparin binding to vitronectin according to classical linkage theory indicates that a single ionic bond is prominent. It had been previously shown …
The Behavior Of Inorganic Material In Biomass-Fired Power Boilers: Field And Laboratory Experiences, Larry Lin Baxter 8507091, Thomas R. Miles, Thomas R. Miles Jr, Bryan M. Jenkins, Thomas Milne, David Dayton, Richard W. Bryers, Larry L. Oden
The Behavior Of Inorganic Material In Biomass-Fired Power Boilers: Field And Laboratory Experiences, Larry Lin Baxter 8507091, Thomas R. Miles, Thomas R. Miles Jr, Bryan M. Jenkins, Thomas Milne, David Dayton, Richard W. Bryers, Larry L. Oden
Faculty Publications
This paper highlights some of the major findings of the Alkali Deposits Investigation, a collaborative effort to understand the causes of unmanageable ash deposits in biomass-fired electric power boilers. A group of interested industrial institutions and the US DOE Energy Efficiency and Renewable Energy Office's Biomass Power Program through the National Renewable Energy Laboratory jointly sponsored the project. The industries contributed both funding and, in most cases, use of facilities to the project and included Mendota Biomass Power and Woodland Biomass Power (both associated with Thermo Electron Energy Systems), CMS Generation Operating (formerly Hydra-Co Operations), Wheelabrator, Shasta and Hudson Energy, …
Combustion Properties Of Biomass, B. M. Jenkins, Larry Lin Baxter 8507091, T. R. Miles Jr, T. R. Miles
Combustion Properties Of Biomass, B. M. Jenkins, Larry Lin Baxter 8507091, T. R. Miles Jr, T. R. Miles
Faculty Publications
Properties of biomass relevant to combustion are briefly reviewed. The compositions of biomass among fuel types are variable, especially with respect to inorganic constituents important to the critical problems of fouling and slagging. Alkali and alkaline earth metals, in combination with other fuel elements such as silica and sulfur, and facilitated by the presence of chlorine, are responsible for many undesirable reactions in combustion furnaces and power boilers. Reductions in the concentrations of alkali metals and chlorine, created by leaching the elements from the fuel with water, yield remarkable improvements in ash fusion temperatures and confirm much of what is …
Erratum: Lizards Took Express Train To Polynesia (Nature (1999) 397 (13- 114), C. Austin
Erratum: Lizards Took Express Train To Polynesia (Nature (1999) 397 (13- 114), C. Austin
Faculty Publications
No abstract provided.
Crystal Structure Of The H256a Mutant Of Rat Testis Fructose-6- Phosphate,2-Kinase/Fructose-2,6-Bisphosphatase: Fructose 6-Phosphate In The Active Site Leads To Mechanisms For Both Mutant And Wild Type Bisphosphatase Activities, Mi H. Yuen, Hiroyuki Mizuguchi, Yong Hwan Lee, Paul F. Cook, Kosaku Uyeda, Charles A. Hasemann
Crystal Structure Of The H256a Mutant Of Rat Testis Fructose-6- Phosphate,2-Kinase/Fructose-2,6-Bisphosphatase: Fructose 6-Phosphate In The Active Site Leads To Mechanisms For Both Mutant And Wild Type Bisphosphatase Activities, Mi H. Yuen, Hiroyuki Mizuguchi, Yong Hwan Lee, Paul F. Cook, Kosaku Uyeda, Charles A. Hasemann
Faculty Publications
Fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase (Fru-6-P,2- kinase/Fru-2,6-Pase) is a bifunctional enzyme, catalyzing the interconversion of β-D-fructose-6-phosphate (Fru-6-P) and fructose-2,6-bisphosphate (Fru- 2,6-P2) at distinct active sites. A mutant rat testis isozyme with an alanine replacement for the catalytic histidine (H256A) in the Fru-2,6-Pase domain retains 17% of the wild type activity (Mizuguchi, H., Cook, P. F., Tai, C-H., Hasemann, C. A., and Uyeda, K. (1998) J. Biol. Chem. 274, 2166- 2175). We have solved the crystal structure of H256A to a resolution of 2.4 Å by molecular replacement. Clear electron density for Fru-6-P is found at the Fru-2,6-Pase active site, revealing the important interactions in …
Rainfall Stimulation Of Primary Production In Western Atlantic Ocean Waters: Roles Of Different Nitrogen Sources And Co-Limiting Nutrients, Hans W. Paerl, Joan D. Willey, Malia Go, Benjamin L. Peierls, James L. Pinckney, Marilyn L. Fogel
Rainfall Stimulation Of Primary Production In Western Atlantic Ocean Waters: Roles Of Different Nitrogen Sources And Co-Limiting Nutrients, Hans W. Paerl, Joan D. Willey, Malia Go, Benjamin L. Peierls, James L. Pinckney, Marilyn L. Fogel
Faculty Publications
Using shipboard bioassays, we examined the roles rainfall, individual and combined nutrients play in accelerating primary production in coastal, Gulf Stream and pelagic (Sargasso Sea) locations in the North Atlantic Ocean off North Carolina, USA, from 1993 to 1995. Photosynthetic CO2 fixation and net chlorophyll a (chl a) production were measured In replicated bioassays to assess individual and combined impacts of different constituents of atmospheric deposition, including natural rainfall, a synthetic rain mix, dissolved inorganic nitrogen (DIN; NH4+ ,NO3-), dissolved organic nitrogen (DON; urea),phosphorus (PO43-) and iron (as EDTA-chelated and …
Interaction Of Soil Moisture And Seedling Shelters On Water Relations Of Baldcypress Seedlings, Ty Swirin, Hans Williams, Bob Keeland
Interaction Of Soil Moisture And Seedling Shelters On Water Relations Of Baldcypress Seedlings, Ty Swirin, Hans Williams, Bob Keeland
Faculty Publications
Stomata1 conductance, transpiration, and leaf water potential were measured during the 1996 growing season on baldcypress (Taxodium disfichum (L.) Rich.) seedlings. Seedlings were hand-planted from 1-O bareroot stock in mesic and permanently Rooded soil conditions. One-half of all seedlings were fitted with 122-cm tall polyethylene tree shelters, Seedlings were planted 1 year before the initiation of plant water relation measurements. The study was located within the boundary of the Longhorn Army Ammunition Plant, Kamack, TX. The objective of the research was to study the feasibility of artificially regenerating baldcypress along the shores of Caddo Lake. Stomata1 conductance and transpiration …
Caudal Distraction By Rat Snakes (Colubridae, Elaphe): A Novel Behavior Used When Capturing Mammalian Prey, Stephen J. Mullin
Caudal Distraction By Rat Snakes (Colubridae, Elaphe): A Novel Behavior Used When Capturing Mammalian Prey, Stephen J. Mullin
Faculty Publications
Caudal movement in snakes may serve either a predatory (e.g., caudal luring) or defensive (eg., rattling, aposematism) function. I describe a new behavioral pattern of tail movement in snakes. Gray rat snakes (Elaphe obsoleta spiloides) foraging on small mammals (Mus domesticus) moved their tails in an erratic, whiplike fashion after detecting their prey in the vicinity. The thrashing movement in the horizontal plane was audibly and visually obvious, resulting in displacement of leaf litter around the tail. All subjects displayed the behavior, but not in all foraging episodes. Shorter durations of caudal distraction resulted in greater …
Does Nonrandom Nest Placement Imply Nonrandom Nest Predation?: A Reply, Robert J. Cooper, R. Randy Wilson, Gary D. Zenitsky, Stephen J. Mullin, Jennifer A. Dececco, Matthew R. Marshall, Dorothy J. Wolf, Lars Y. Pomara
Does Nonrandom Nest Placement Imply Nonrandom Nest Predation?: A Reply, Robert J. Cooper, R. Randy Wilson, Gary D. Zenitsky, Stephen J. Mullin, Jennifer A. Dececco, Matthew R. Marshall, Dorothy J. Wolf, Lars Y. Pomara
Faculty Publications
In response to the critique by Schmidt and Whelan (Condor 101 (4): 916-920, 1999), we find that the relationship between nest success and tree selectivity is dependent upon inclusion or exclusion of particular tree species, whether or not years are pooled, and the selectivity index used. We question their use of point estimates of nest success with extremely high variances, defend our index, question the application of the Chesson (1983) index to our data, and explain the need to analyze years separately. Bottomland hardwood forest systems are extremely variable; hydroperiods alter the suitability of nesting substrates, availability of alternative food, …
Iron-Induced Oxidative Stress In Erythrocyte Membranes Of Non-Insulin-Dependent Diabetic Nigerians, Gbolahan W. Okunade, Odutayo O. Odunuge, Olufunso O. Olorunsogo
Iron-Induced Oxidative Stress In Erythrocyte Membranes Of Non-Insulin-Dependent Diabetic Nigerians, Gbolahan W. Okunade, Odutayo O. Odunuge, Olufunso O. Olorunsogo
Faculty Publications
The presence of higher level of endogenous free radical reaction products in the erythrocyte ghost membrane (EGM) of Non-insulin-dependent diabetes mellitus (NIDDM) subjects compared with that of normal healthy controls has been demonstrated. The EGMs of NIDDM subjects were also shown to be more susceptible to exogenously generated oxidative stress than those of normal healthy individuals. The decreased level of reactive thiol groups in the EGM of NIDDM individuals supported this observation. We propose that the presence of significant levels of non-heme iron in the EGM of NIDDM subjects is an indication of the potential for iron-catalysed production of hydroxy …
Reversal Of Sodium Arsenite Inhibition Of Rat Liver Microsomal Ca2+ Pumping Atpase By Vitamin C, Odutayo O. Odunuge, Gbolahan W. Okunade, Olufunso O. Olorunsogo
Reversal Of Sodium Arsenite Inhibition Of Rat Liver Microsomal Ca2+ Pumping Atpase By Vitamin C, Odutayo O. Odunuge, Gbolahan W. Okunade, Olufunso O. Olorunsogo
Faculty Publications
Sodium arsenite (NaAsO2), at 10% of its median lethal dose, was administered to rats with and without vitamin C pretreatment. Liver microsomal fraction was isolated and the activity of Ca2+-ATPase was assayed. Sodium arsenite was found to inhibit the activity of the liver microsomal Ca2+-ATPase to 50% to that of control rats. The specific activity of the enzyme in rats administered sodium arsenite with vitamin C pretreatment was not significantly different from that of control rats.
Relationships Of Selected Soil Properties And Community Species Composition In Turkey Hill Wilderness Area In East Texas, Kenneth W. Farrish, Brian P. Oswald
Relationships Of Selected Soil Properties And Community Species Composition In Turkey Hill Wilderness Area In East Texas, Kenneth W. Farrish, Brian P. Oswald
Faculty Publications
-Sixty-nine different stands within 15 different previously managed communities were sampled in what is now the Turkey Hill Wilderness of the Angelina National Forest. Within each stand, 0.04 ha plots were randomly located, and the height, diameter, crown class and species of each tree recorded. Soil samples were collected in two locations within each plot, and the soil type confirmed at plot center. Soil samples were analyzed for selected soil chemical and physical properties. Due to the low occurrence (less than 4 times) of some communities, only six community types were analyzed Analysis of the vegetation and soil data using …
Management Of Droughty Site: Typic Quartzipsamments, Ecological Considerations, David Kulhavy, W. G. Ross, L. Allen Smith, R. R. Cahal
Management Of Droughty Site: Typic Quartzipsamments, Ecological Considerations, David Kulhavy, W. G. Ross, L. Allen Smith, R. R. Cahal
Faculty Publications
Pine plantations on Typic Quartzipsamments in East Texas are difficult to establish. Forest management options following clearcutting are limited. A 12 year regeneration study of the growth and survival of loblolly, Pinus faeda, L. shortleaf, P. echinata Mill., slash, f. elliofii Engelm and longleaf pines P. pa/u&is Mill. was conducted to determine optimum tree species and treatments for reforestation. With successful regeneration also comes insects and pathogens. Impacts of the Nantucket pine tip moth, Rhyacionia frusfrana, (Comstock) and the Texas leaf-cutting ant, Affa texana, (Buckley) will be discussed in the context of droughty site management.
Changes In Understory Woody Vegetation In Red-Cockaded Woodpecker (Picoides Borealis) Nesting Clusters, W. D. Hacker, W. G. Ross, David Kulhavy
Changes In Understory Woody Vegetation In Red-Cockaded Woodpecker (Picoides Borealis) Nesting Clusters, W. D. Hacker, W. G. Ross, David Kulhavy
Faculty Publications
Understory woody vegetation in red-cockaded woodpecker nesting clusters located in Pinus taeda (L.) and P. echinara (Mill.) dominated stands were inventoried from December 1986 through January 1987 and later in January 1995 to determine what effects court-ordered management schemes had on the composition, abundance, and diversity of these stands. There was a significant difference (Pin the composition between the first sampling period and the second with species diversity increasing and a change from a
P. (aeda/P. echinara dominated community to a P. weda/Rubus spp. understory
Seedling Survival And Natural Regeneration For A Bottomland Hardwood Planting On Sites Differing In Site Preparation, Daniel T. Johns, Brett Williams, Hans M. Williams, Matthew Stroupe
Seedling Survival And Natural Regeneration For A Bottomland Hardwood Planting On Sites Differing In Site Preparation, Daniel T. Johns, Brett Williams, Hans M. Williams, Matthew Stroupe
Faculty Publications
In January 1998, three tracts in Hardin County, TX, were hand-planted with seven species of 1-0 bareroot bottomland hardwood seedlings. The tracts, managed by The Nature Conservancy of Texas, were previously 20-year-old pine plantations. The tracts are located within the floodplain of Village Creek. An objective for this conversion is the restoration of a bottomland hardwood wetland in order to meet Clean Water Act requirements. A pre-harvest plant inventory was conducted for each tract. The tracts were clearcut during the Winter and Spring of 1997. Following harvest, each tract was subjected to a different site preparation technique. One tract was …
Some Aspects Of The Adsorption Of A Lennard-Jones Gas On A Rough Surface, Douglas Henderson, Pawel Bryk, Stefan Sokolowski
Some Aspects Of The Adsorption Of A Lennard-Jones Gas On A Rough Surface, Douglas Henderson, Pawel Bryk, Stefan Sokolowski
Faculty Publications
We study the adsorption, including wetting, of a Lennard-Jones gas on a rough surface consisting of rough layer on a smooth substrate with which the gas interacts via a 9-3 potential. The rough layer is two molecular diameters thick and consists of a disordered quenched matrix of hard spheres. As well as interacting with the other matrix spheres by the hard-sphere potential, the matrix molecules interact with the gas molecules by the hard-sphere potential. Hence, on average, the degree to which the gas molecules can approach the substrate depends only on the density of the matrix layer. The density of …
Biochemical And Biophysical Analysis Of The Intracellular Lipid Binding Proteins Of Adipocytes, Melanie A. Simpson, Vince J. Licata, Natalie Ribarik Coe, David A. Bernlohr
Biochemical And Biophysical Analysis Of The Intracellular Lipid Binding Proteins Of Adipocytes, Melanie A. Simpson, Vince J. Licata, Natalie Ribarik Coe, David A. Bernlohr
Faculty Publications
Adipocytes express two lipid-binding proteins; the major one termed the adipocyte lipid-binding protein or aP2 (ALBP/aP2) and a minor one referred to as the keratinocyte lipid-binding protein (KLBP). In order to evaluate the potential physiological roles for these proteins, their biochemical and biophysical properties have been analyzed and compared. ALBP/aP2 and KLBP exhibit similar binding affinities for most long-chain fatty acids; however, ALBP/aP2 exhibits a two to three-fold increased affinity for myristic, palmitic, oleic and linoleic acids, the predominant fatty acids of adipocytes. As measured by guanidinium hydrochloride denaturation, the stability of ALBP/aP2 is nearly 3 kcal/mol greater than that …
Hydrostatic Pressure Alters The Time Course Of Gtp[S] Binding To G Proteins In Brain Membranes From Two Congeneric Marine Fishes, Joseph F. Siebenaller, Thomas F. Murray
Hydrostatic Pressure Alters The Time Course Of Gtp[S] Binding To G Proteins In Brain Membranes From Two Congeneric Marine Fishes, Joseph F. Siebenaller, Thomas F. Murray
Faculty Publications
The effects of hydrostatic pressure on the receptor-stimulated exchange of guanosine triphosphate (GTP) for guanosine diphosphate (GDP) on the α subunit of G proteins were studied in two congeneric marine teleost fishes that differ in their depths of distribution. The poorly hydrolyzable GTP analog [35S]guanosine 5'-[γ-thio]triphosphate ([35S]GTP[S]) was used to monitor the modulation of signal transduction by the A(I) adenosine receptor agonist N6-R-(phenylisopropyl)adenosine (R-PIA) in brain membranes of the scorpaenids Sebastolobus alascanus and S. altivelis. The maximal binding (B(max)) and dissociation constant (K(d)) values, determined from equilibrium binding isotherms at atmospheric pressure (5°C), were similar in the two species. The …
Protein And Ligand Adaptation In A Retinoic Acid Binding Protein, Rekha Pattanayek, Marcia E. Newcomer
Protein And Ligand Adaptation In A Retinoic Acid Binding Protein, Rekha Pattanayek, Marcia E. Newcomer
Faculty Publications
A retinoic acid binding protein isolated from the lumen of the rat epididymis (ERABP) is a member of the lipocalin superfamily. ERABP binds both the all-trans and 9-cis isomers of retinoic acid, as well as the synthetic retinoid (E)-4-[2-(5,6,7,8)-tetrahydro-5,5,8,8-tetramethyl-2 napthalenyl-1 propenyl]-benzoic acid (TTNPB), a structural analog of all-trans retinoic acid. The structure of ERABP with a mixture of all-trans and 9-cis retinoic acid has previously been reported. To elucidate any structural differences in the protein when bound to the all-trans and 9-cis isomers, the structures of all-trans retinoic acid-ERABP and 9-cis retinoic acid ERABP were determined. Our results indicate that …
Attributes Of Atmospheric Carbon Monoxide Oxidation By Maine Forest Soils, G. M. King
Attributes Of Atmospheric Carbon Monoxide Oxidation By Maine Forest Soils, G. M. King
Faculty Publications
CO, one of the most important trace gases, regulates tropospheric methane, hydroxyl radical, and ozone contents. Ten to 25% of the estimated global CO flux may be consumed by soils annually. Depth profiles for 14CO oxidation and CO concentration indicated that CO oxidation occurred primarily in surface soils and that photooxidation of soil organic matter did not necessarily contribute significantly to CO fluxes. Kinetic analyses revealed that the apparent K(m) was about 18 nM (17 ppm) and the V(max) was 6.9 μmol g (fresh weight)-1 h-1; the apparent K(m) was similar to the apparent K(m) for atmospheric methane consumption, but …
Costs Of Induced Responses And Tolerance To Herbivory In Male And Female Fitness Components Of Wild Radish, A. A. Agrawal, S. Y. Strauss, M. J. Stout
Costs Of Induced Responses And Tolerance To Herbivory In Male And Female Fitness Components Of Wild Radish, A. A. Agrawal, S. Y. Strauss, M. J. Stout
Faculty Publications
Theory predicts that plant defensive traits are costly due to trade-offs between allocation to defense and growth and reproduction. Most previous studies of costs of plant defense focused on female fitness costs of constitutively expressed defenses. Consideration of alternative plant strategies, such as induced defenses and tolerance to herbivory, and multiple types of costs, including allocation to male reproductive function, may increase our ability to detect costs of plant defense against herbivores. In this study we measured male and female reproductive costs associated with induced responses and tolerance to herbivory in annual wild radish plants (Raphanus raphanistrum). We induced resistance …
Genetic Analysis Of The Role Of Herpes Simplex Virus Type 1 Glycoprotein K In Infectious Virus Production And Egress, Timothy P. Foster, Konstantin G. Kousoulas
Genetic Analysis Of The Role Of Herpes Simplex Virus Type 1 Glycoprotein K In Infectious Virus Production And Egress, Timothy P. Foster, Konstantin G. Kousoulas
Faculty Publications
Herpes simplex virus type 1 (KOS)ΔgK is a mutant virus which lacks glycoprotein K (gK) and exhibits defects in virion egress (S. Jayachandra, A. Baghian, and K. G. Kousoulas, J. Virol. 69:5401-5413, 1997). To further understand the role of gK in virus egress, we constructed recombinant viruses, ΔgKhpd-1, -2, -3, and -4, that specified gK amino-terminal portions of 139, 239, 268, and 326 amino acids, respectively, corresponding to truncations immediately after each of the four putative membrane-spanning domains of gK. ΔgKhpd-1 and ΔgKhpd-2 viruses produced lower yields and smaller plaques than ΔgK. Numerous ΔgKhpd-1 capsids accumulated predominately within large double-membrane …
Use Of Western Immunoblot Analysis For Testing Moose Serum For Brucella Suis Biovar 4 Specific Antibodies, Matthew D. Edmonds, Felicia M. Ward, Todd M. O'Hara, Philip H. Elzer
Use Of Western Immunoblot Analysis For Testing Moose Serum For Brucella Suis Biovar 4 Specific Antibodies, Matthew D. Edmonds, Felicia M. Ward, Todd M. O'Hara, Philip H. Elzer
Faculty Publications
To determine if 12 moose (Alces alces) from northern Alaska with agglutinating antibodies specific for Brucella spp. had been exposed to either B. suis biovar 4 or B. abortus biovar 1, western immunoblot serologic analysis was performed. Differential serologic responses to strain specific A and M antigenic variances of the lipopolysaccharide O-polysaccharide sugar allowed strain identification. Prior to examination, test sera were absorbed with killed whole cells from either B. abortus biovar 1, containing predominately A antigen (A+ M-); B. melitensis biovar 1, containing essentially M antigen (A-M+); or B. suis biovar 4, containing both antigenic types (A+ M+). The …
The Siderophore 2,3-Dihydroxybenzoic Acid Is Not Required For Virulence Of Brucella Abortus In Balb/C Mice, Bryan H. Bellaire, Philip H. Elzer, Cynthia L. Baldwin, R. Martin Roop
The Siderophore 2,3-Dihydroxybenzoic Acid Is Not Required For Virulence Of Brucella Abortus In Balb/C Mice, Bryan H. Bellaire, Philip H. Elzer, Cynthia L. Baldwin, R. Martin Roop
Faculty Publications
2,3-Dihydroxybenzoic acid (DHBA) is the only siderophore described for Brucella, and previous studies suggested that DHBA might contribute to the capacity of these organisms to persist in host macrophages. Employing an isogenic siderophore mutant (ΔentC) constructed from virulent Brucella abortus 2308, however, we found that production of DHBA is not required for replication in cultured murine macrophages or for the establishment and maintenance of chronic infection in the BALB/c mouse model.