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Comparing Regeneration Techniques For Afforesting Previously Farmed Bottomland Hardwood Sites In The Lower Mississippi Alluvial Valley, Usa, Brian Roy Lockhart, Bob Keeland, John Mccoy, Thomas J. Dean May 2003

Comparing Regeneration Techniques For Afforesting Previously Farmed Bottomland Hardwood Sites In The Lower Mississippi Alluvial Valley, Usa, Brian Roy Lockhart, Bob Keeland, John Mccoy, Thomas J. Dean

Faculty Publications

A study was implemented to test site preparation methods and artificial regeneration of three oak (Quercus spp.) species on four agricultural fields in the Lower Mississippi Alluvial Valley in Louisiana, USA. Six years after establishment, few consistent differences were found in oak density between sowing acorn methods (seed drill versus broadcast seeding), autumn sowing versus spring sowing, and sowing acorns versus planting oak seedlings. Results indicated that some degree of site preparation is needed to establish oak seedlings but few differences were found between site preparation treatments. These results indicate that no one prescription for oak regeneration fits all potential …

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Predicting Irregularities In Population Cycles, Shandelle M. Henson, James R. Reilly, Suzanne L. Robertson, Matthew C. Schu, Eric W. D. Rozier, J. M. Cushing May 2003

Predicting Irregularities In Population Cycles, Shandelle M. Henson, James R. Reilly, Suzanne L. Robertson, Matthew C. Schu, Eric W. D. Rozier, J. M. Cushing

Faculty Publications

Oscillating population data often exhibit cycle irregularities such as episodes of damped oscillation and abrupt changes of cycle phase. The prediction of such irregularities is of interest in applications ranging from food production to wildlife management. We use concepts from dynamical systems theory to present a model-based method for quantifying the risk of impending cycle irregularity.

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A Noise Filtering Method Using Neural Networks, Tony R. Martinez, Xinchuan Zeng May 2003

A Noise Filtering Method Using Neural Networks, Tony R. Martinez, Xinchuan Zeng

Faculty Publications

During the data collecting and labeling process it is possible for noise to be introduced into a data set. As a result, the quality of the data set degrades and experiments and inferences derived from the data set become less reliable. In this paper we present an algorithm, called ANR (automatic noise reduction), as a filtering mechanism to identify and remove noisy data items whose classes have been mislabeled. The underlying mechanism behind ANR is based on a framework of multi-layer artificial neural networks. ANR assigns each data item a soft class label in the form of a class probability …

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Production Of Methyl Bromide In A Temperate Forest Soil, Ruth K. Varner, Marguerite L. White, Cindy H. Mosedale, Patrick M. Crill May 2003

Production Of Methyl Bromide In A Temperate Forest Soil, Ruth K. Varner, Marguerite L. White, Cindy H. Mosedale, Patrick M. Crill

Faculty Publications

Field enclosure measurements of a temperate forest soil show net uptake of ambient methyl bromide (CH3Br), an important trace gas in both tropospheric and stratospheric ozone cycling. The net flux for 1999 was estimated to be −168 ± 72 μg CH3Br m−2 (negative indicates loss from the atmosphere). Individual enclosure flux measurements ranged from −4.0 to +3.3 μg CH3Br m−2 d−1. Soil consumption of CH3Br was estimated from laboratory soil incubations. Production of CH3Br was calculated as the difference between net flux and predicted consumption. Fungi could be responsible for the production of CH3Br in this temperate forest soil.

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High Affinity Ligand Binding By Integrins Does Not Involve Head Separation, Bing Hao Luo, Timothy A. Springer, Junichi Takagi May 2003

High Affinity Ligand Binding By Integrins Does Not Involve Head Separation, Bing Hao Luo, Timothy A. Springer, Junichi Takagi

Faculty Publications

Conformational change in the integrin extracellular domain is required for high affinity ligand binding and is also involved in post-ligand binding cellular signaling. Although there is evidence to the contrary, electron microscopic studies showing that ligand binding triggers α- and β-subunit dissociation in the integrin head-piece have gained popularity and support the hypothesis that head separation activates integrins. To test directly the head separation hypothesis, we enforced head association by introducing disulfide bonds across the interface between the α-subunit β-propeller domain and the β-subunit I-like domain. Basal and activation-dependent ligand binding by αIIbβ3 and αVβ3 was unaffected. The covalent linkage …

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Mercury Concentrations In Streams Of East Texas, Mingteh Chang, Mark C. Cochran, R. Scott Beasley, Matthew W. Mcbroom May 2003

Mercury Concentrations In Streams Of East Texas, Mingteh Chang, Mark C. Cochran, R. Scott Beasley, Matthew W. Mcbroom

Faculty Publications

Recent studies on potential mercury (Hg) contamination of fish from East Texas lakes and waterways have caused concern about mercury levels in East Texas waters. Historical records of Hg concentrations in 33 East Texas streams showed that median concentrations for each stream segment were no different than other U.S. streams. All the means and medians for stream segments having at least 20 recorded measurements were less than Texas (2.4 µg/L) water quality standards. Water samples collected in December 1995 and March 1996 from 6 different stream sites in Nacogdoches County had concentrations similar to historical records. Due to biological magnification, …

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Evidence That Acetyl Phosphate Functions As A Global Signal During Biofilm Development, Alan J. Wolfe, Dong Eun Chang, Jason D. Walker, Jeanine E. Seitz-Partridge, Michael D. Vidaurri, Charles F. Lange, Birgit M. Prüß, Margaret C. Henk, John C. Larkin, Tyrrell Conway May 2003

Evidence That Acetyl Phosphate Functions As A Global Signal During Biofilm Development, Alan J. Wolfe, Dong Eun Chang, Jason D. Walker, Jeanine E. Seitz-Partridge, Michael D. Vidaurri, Charles F. Lange, Birgit M. Prüß, Margaret C. Henk, John C. Larkin, Tyrrell Conway

Faculty Publications

We used DNA macroarray analysis to identify genes that respond to the status of the intracellular acetyl phosphate (acP) pool. Genes whose expression correlated negatively with the ability to synthesize acP (i.e. negatively regulated genes) function primarily in flagella biosynthesis, a result consistent with observations that we published previously (Prüß and Wolfe, 1994, Mol Microbiol 12: 973-984). In contrast, genes whose expression correlated positively with the ability to synthesize acP (i.e. positively regulated genes) include those for type 1 pilus assembly, colanic acid (capsule) biosynthesis and certain stress effectors. To our knowledge, this constitutes the first report that these genes …

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Production Of The Siderophore 2,3-Dihydroxybenzoic Acid Is Required For Wild-Type Growth Of Brucella Abortus In The Presence Of Erythritol Under Low-Iron Conditions In Vitro, Bryan H. Bellaire, Philip H. Elzer, Cynthia L. Baldwin, R. Martin Roop May 2003

Production Of The Siderophore 2,3-Dihydroxybenzoic Acid Is Required For Wild-Type Growth Of Brucella Abortus In The Presence Of Erythritol Under Low-Iron Conditions In Vitro, Bryan H. Bellaire, Philip H. Elzer, Cynthia L. Baldwin, R. Martin Roop

Faculty Publications

Production of the siderophore 2,3-dihyroxybenzoic acid (2,3-DHBA) is required for the wild-type virulence of Brucella abortus in cattle. A possible explanation for this requirement was uncovered when it was determined that a B. abortus dhbC mutant (BHB1) defective in 2,3-DHBA production displays marked growth restriction in comparison to its parent strain, B. abortus 2308, when cultured in the presence of erythritol under low-iron conditions. This phenotype is not displayed when these strains are cultured under low-iron conditions in the presence of other readily utilizable carbon and energy sources. The addition of either exogenous 2,3-DHBA or FeCl3 relieves this growth defect, …

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The Utility Of Single Nucleotide Polymorphisms In Inferences Of Population History, Robb T. Brumfield, Peter Beerli, Deborah A. Nickerson, Scott V. Edwards May 2003

The Utility Of Single Nucleotide Polymorphisms In Inferences Of Population History, Robb T. Brumfield, Peter Beerli, Deborah A. Nickerson, Scott V. Edwards

Faculty Publications

Single nucleotide polymorphisms (SNPs) represent the most widespread type of sequence variation in genomes, yet they have only emerged recently as valuable genetic markers for revealing the evolutionary history of populations. Their occurrence throughout the genome also makes them ideal for analyses of speciation and historical demography, especially in light of recent theory suggesting that many unlinked nuclear loci are needed to estimate population genetic parameters with statistical confidence. In spite of having lower variation compared with microsatellites, SNPs should make the comparison of genomic diversities and histories of different species (the core goal of comparative biogeography) more straightforward than …

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Linkage For Bmi At 3q27 Region Confirmed In An African-American Population, Amy Luke, Xiaodong Wu, Xiaofeng Zhu, Donghui Kan, Yan Su, Richard Cooper May 2003

Linkage For Bmi At 3q27 Region Confirmed In An African-American Population, Amy Luke, Xiaodong Wu, Xiaofeng Zhu, Donghui Kan, Yan Su, Richard Cooper

Faculty Publications

Significant and suggestive linkage for BMI on 3q27 has been reported by several groups, including our own study in African Americans. To further establish the linkage evidence on 3q27, we recruited an independent African-American sample comprising 545 individuals in 128 families. We genotyped 15 short tandem-repeat markers evenly spaced in the 112 cM region around the peak on 3q27 identified in our earlier study. Multipoint linkage analysis by GENEHUNTER2 gave the maximum logarithm of odds (LOD) score 2.4 at map position 188 cM in this sample. When we combined the two samples, linkage evidence was increased to a maximum LOD …

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Associations Between Hypertension And Genes In The Renin-Angiotensin System, Xiaofeng Zhu, Yen Pei C. Chang, Denise Yan, Alan Weder, Richard Cooper, Amy Luke, Donghui Kan, Aravinda Chakravarti May 2003

Associations Between Hypertension And Genes In The Renin-Angiotensin System, Xiaofeng Zhu, Yen Pei C. Chang, Denise Yan, Alan Weder, Richard Cooper, Amy Luke, Donghui Kan, Aravinda Chakravarti

Faculty Publications

The genes of the renin-angiotensin system have been subjected to intense molecular scrutiny in cardiovascular disease studies, but their contribution to risk is still uncertain. In this study, we sampled 192 African American and 153 European American families (602 and 608 individuals, respectively) to evaluate the contribution of variations in genes that encode renin-angiotensin system components of susceptibility to hypertension. We genotyped 25 single-nucleotide polymorphisms in the renin-angiotensin system genes ACE, AGT, AGTR1, and REN. The family-based transmission/disequilibrium test was performed with each single-nucleotide polymorphism and with the multilocus haplotypes. Two individual single-nucleotide polymorphisms were significantly associated with hypertension among …

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Enabling Remote Access To Personal Electronic Medical Records, James K. Archibald, Eric S. Hall, Charles D. Knutson, David K. Vawdrey May 2003

Enabling Remote Access To Personal Electronic Medical Records, James K. Archibald, Eric S. Hall, Charles D. Knutson, David K. Vawdrey

Faculty Publications

Millions of people suffer from medical conditions that should be made known to healthcare practitioners prior to treatment. Paramedics and emergency room doctors cannot provide optimal care without sufficient knowledge of a patient’s medical history. Lacking patient information such as allergies, current prescriptions, and preexisting conditions, medical professionals are often forced to either delay treatment or rely on instincts. Medical mistakes in situations like these kill thousands of people and cost an estimated US$37 billion each year in the United States [1]. With the advent of electronic medical records (EMRs), patient information can be stored in computer databases at hospitals …

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Evidence For Survival Of Pleistocene Climatic Changes In Northern Refugia By The Land Snail Trochoidea Geyeri, David Posada, Markus Pfenninger, Frederic Magnin Apr 2003

Evidence For Survival Of Pleistocene Climatic Changes In Northern Refugia By The Land Snail Trochoidea Geyeri, David Posada, Markus Pfenninger, Frederic Magnin

Faculty Publications

The study of organisms with restricted dispersal abilities and presence in the fossil record is particularly adequate to understand the impact of climate changes on the distribution and genetic structure of species. Trochoidea geyeri (Soos 1926) is a land snail restricted to a patchy, insular distribution in Germany and France. Fossil evidence suggests that current populations of T. geyeri are relicts of a much more widespread distribution during more favourable climatic periods in the Pleistocene. Results: Phylogeographic analysis of the mitochondrial 16S rDNA and nuclear ITS-1 sequence variation was used to infer the history of the remnant populations of T. …

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Normal Proliferation And Differentiation Of Hoxc-8 Transgenic Chondrocytes In Vitro, Stephania A. Cormier, Maria Alice Mello, Claudia Kappen Apr 2003

Normal Proliferation And Differentiation Of Hoxc-8 Transgenic Chondrocytes In Vitro, Stephania A. Cormier, Maria Alice Mello, Claudia Kappen

Faculty Publications

Background: Hox genes encode transcription factors that are involved in pattern formation in the skeleton, and recent evidence suggests that they also play a role in the regulation of endochondral ossification. To analyze the role of Hoxc-8 in this process in more detail, we applied in vitro culture systems, using high density cultures of primary chondrocytes from neonatal mouse ribs. Results: Cultured cells were characterized on the basis of morphology (light microscopy) and production of cartilage-specific extracellular matrix (sulfated proteoglycans and type II Collagen). Hypertrophy was demonstrated by increase in cell size, alkaline phosphatase activity and type X Collagen immunohistochemistry. …

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Search And Recovery Of The Space Shuttle Columbia: A Geospatial 1st Responder Perspective, Jeffrey M. Williams Apr 2003

Search And Recovery Of The Space Shuttle Columbia: A Geospatial 1st Responder Perspective, Jeffrey M. Williams

Faculty Publications

A first person account of the Texas geospatial volunteers and their efforts to recover the remains of the Space Shuttle Columbia and her crew lost over eastern Texas and western Louisiana on February 1st, 2003.

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Valuation Of Continuous And Intermittent Phragmites Control, R. Eugene Turner, R. S. Warren Apr 2003

Valuation Of Continuous And Intermittent Phragmites Control, R. Eugene Turner, R. S. Warren

Faculty Publications

Quantifying the relative value of Phragmites australis and Spartina alterniflora habitat is important to evaluate the benefits and risks of different attempts to address Phragmites expansion on the U.S. eastern seaboard. Two contrasting approaches commonly used to restore tidal marsh habitats invaded by Phragmites communities involve spraying Phragmites with herbicide only when its coverage of a particular marsh area is near or close to 100%. Alternatively, after the first application, herbicide is annually applied on any surviving patches of Phragmites present in a mosaic of other marsh vegetation. A model is introduced to evaluate the relative habitat value of these …

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Genetic Organization And Iron-Responsive Regulation Of The Brucella Abortus 2,3-Dihydroxybenzoic Acid Biosynthesis Operon, A Cluster Of Genes Required For Wild-Type Virulence In Pregnant Cattle, Bryan H. Bellaire, Philip H. Elzer, Sue Hagius, Joel Walker, Cynthia L. Baldwin, R. Martin Roop Apr 2003

Genetic Organization And Iron-Responsive Regulation Of The Brucella Abortus 2,3-Dihydroxybenzoic Acid Biosynthesis Operon, A Cluster Of Genes Required For Wild-Type Virulence In Pregnant Cattle, Bryan H. Bellaire, Philip H. Elzer, Sue Hagius, Joel Walker, Cynthia L. Baldwin, R. Martin Roop

Faculty Publications

Brucella abortus reportedly produces the monocatechol siderophore 2,3-dihydroxybenzoic acid (2,3-DHBA) in response to iron limitation. Nucleotide sequence analysis of the cloned DHBA biosynthesis locus from virulent B. abortus 2308 and genetic complementation of defined Escherichia coli mutants were used to identify the B. abortus genes (designated dhbC, -B, and -A) responsible for synthesis of this siderophore. Reverse transcriptase PCR analysis of total RNA with dhb-specific primers demonstrated that dhbC, -B, and -A are transcribed as components of an operon, together with dhbE, a functional homolog of the Escherichia coli entE gene. Homologs of the E. coli entD and Vibrio cholerae …

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Interstrain Inhibition In The Sweet Potato Pathogen Streptomyces Ipomoeae: Purification And Characterization Of A Highly Specific Bacteriocin And Cloning Of Its Structural Gene, Xiujun Zhang, Christopher A. Clark, Gregg S. Pettis Apr 2003

Interstrain Inhibition In The Sweet Potato Pathogen Streptomyces Ipomoeae: Purification And Characterization Of A Highly Specific Bacteriocin And Cloning Of Its Structural Gene, Xiujun Zhang, Christopher A. Clark, Gregg S. Pettis

Faculty Publications

Strains of the sweet potato soil rot pathogen Streptomyces ipomoeae had previously been divided into three groups based on their ability to inhibit one another during pairwise cocultivation. While group I strains are not antagonistic to members of the other groups, group II and group III strains produce separate substances that are inhibitory to strains outside their respective cognate groups. Here, we purified the group III inhibitory substance from the culture supernatant of a representative strain and found that it consists of a single 10-kDa cationic protein which is bacteriolytic for S. ipomoeae group I and II strains but which …

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Y Chromosome Str Haplotypes And The Genetic Structures Of U.S. Populations Of African, European, And Hispanic Ancestry, Manfred Kayser, Silke Brauer, Hiltrud Schädlich, Mechthild Prinz, Mark A. Batzer, Peter A. Zimmerman, B. A. Boatin, Mark Stoneking Apr 2003

Y Chromosome Str Haplotypes And The Genetic Structures Of U.S. Populations Of African, European, And Hispanic Ancestry, Manfred Kayser, Silke Brauer, Hiltrud Schädlich, Mechthild Prinz, Mark A. Batzer, Peter A. Zimmerman, B. A. Boatin, Mark Stoneking

Faculty Publications

To investigate geographic structure within U.S. ethnic populations, we analyzed 1705 haplotypes on the basis of 9 short tandem repeat (STR) loci on the Y-chromosome from 9-11 groups each of African-Americans, European-Americans, and Hispanics. There were no significant differences in the distribution of Y-STR haplotypes among African-American groups, whereas European-American and Hispanic groups did exhibit significant geographic heterogeneity. However, the significant heterogeneity resulted from one sample; removal of that sample in each case eliminated the significant heterogeneity. Multidimensional scaling analysis of RST values indicated that African-American groups formed a distinct cluster, whereas there was some intermingling of European-American and Hispanic …

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Aintegumenta Utilizes A Mode Of Dna Recognition Distinct From That Used By Proteins Containing A Single Ap2 Domain, Beth A. Krizek Apr 2003

Aintegumenta Utilizes A Mode Of Dna Recognition Distinct From That Used By Proteins Containing A Single Ap2 Domain, Beth A. Krizek

Faculty Publications

The Arabidopsis protein AINTEGUMENTA (ANT) is an important regulator of organ growth during flower development. ANT is a member of the AP2 subclass of the AP2/ERF family of plant-specific transcription factors. These proteins contain either one or two copies of a DNA-binding domain called the AP2 domain. Here, it is shown that ANT can act as a transcriptional activator in yeast through binding to a consensus ANT-binding site. This activity was used as the basis for a genetic screen to identify amino acids that are critical for the DNA binding ability of ANT. Mutants that showed reduced or no activation …

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The Struggle For School Desegregation In Cincinnati Before 1954, Davison M. Douglas Apr 2003

The Struggle For School Desegregation In Cincinnati Before 1954, Davison M. Douglas

Faculty Publications

No abstract provided.

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Evolution, Theology, And Method - Part 1: Outline And Limits Of Scientific Methodology, Fernando L. Canale Apr 2003

Evolution, Theology, And Method - Part 1: Outline And Limits Of Scientific Methodology, Fernando L. Canale

Faculty Publications

No abstract provided.

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Influence Of A Cyclonic Eddy On Microheterotroph Biomass And Carbon Export In The Lee Of Hawaii, Robert R. Bidigare, Claudia R. Benitez-Nelson, Carrie L. Leonard, Paul D. Quay, Michael L. Parsons, David G. Foley, Michael P. Seki Mar 2003

Influence Of A Cyclonic Eddy On Microheterotroph Biomass And Carbon Export In The Lee Of Hawaii, Robert R. Bidigare, Claudia R. Benitez-Nelson, Carrie L. Leonard, Paul D. Quay, Michael L. Parsons, David G. Foley, Michael P. Seki

Faculty Publications

[1] A multi‐platform sampling strategy was used to investigate carbon cycling in a cold‐core eddy that formed in the lee of Hawaii during September 2000. Microheterotroph biomass and 234Th‐derived carbon export rates within the eddy were 2 to 3 times higher than those observed for adjacent waters. If this eddy is representative of other cyclonic eddies that are frequently formed in the lee of Hawaii, then eddy activity may significantly enhance the areal efficiency of the biological pump and facilitate the transfer of organic carbon to organisms inhabiting the mesopelagic and abyssal‐benthic zones of this subtropical ecosystem.

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A Causative Relationship Exists Between Eosinophils And The Development Of Allergic Pulmonary Pathologies In The Mouse, Hua Hao H. Shen, Sergei I. Ochkur, Michael P. Mcgarry, Jeffrey R. Crosby, Edie M. Hines, Michael T. Borchers, Huiying Wang, Travis L. Biechelle, Katie R. O'Neill, Tracy L. Ansay, Dana C. Colbert, Stephania A. Cormier, J. Paul Justice, Nancy A. Lee, James J. Lee Mar 2003

A Causative Relationship Exists Between Eosinophils And The Development Of Allergic Pulmonary Pathologies In The Mouse, Hua Hao H. Shen, Sergei I. Ochkur, Michael P. Mcgarry, Jeffrey R. Crosby, Edie M. Hines, Michael T. Borchers, Huiying Wang, Travis L. Biechelle, Katie R. O'Neill, Tracy L. Ansay, Dana C. Colbert, Stephania A. Cormier, J. Paul Justice, Nancy A. Lee, James J. Lee

Faculty Publications

Asthma and mouse models of allergic respiratory inflammation are invariably associated with a pulmonary eosinophilia; however, this association has remained correlative. In this report, a causative relationship between eosinophils and allergen-provoked pathologies was established using eosinophil adoptive transfer. Eosinophils were transferred directly into the lungs of either naive or OVA-treated IL-5-/- mice. This strategy resulted in a pulmonary eosinophilia equivalent to that observed in OVA-treated wild-type animals. A concomitant consequence of this eosinophil transfer was an increase in Th2 bronchoalveolar lavage cytokine levels and the restoration of intracellular epithelial mucus in OVA-treated IL-5-/- mice equivalent to OVA-treated wild-type levels. Moreover, …

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Stabilizing The Open Conformation Of The Integrin Headpiece With A Glycan Wedge Increases Affinity For Ligand, Bing Hao Luo, Timothy A. Springer, Junichi Takagi Mar 2003

Stabilizing The Open Conformation Of The Integrin Headpiece With A Glycan Wedge Increases Affinity For Ligand, Bing Hao Luo, Timothy A. Springer, Junichi Takagi

Faculty Publications

The affinity of the extracellular domain of integrins for ligand is regulated by conformational changes signaled from the cytoplasm. Alternative types of conformational movement in the ligandbinding headpiece have been proposed. In one study, electron micrograph image averages of the headpiece of integrin aVβ3 show two different conformations. The open conformation of the headpiece is present when a ligand mimetic peptide is bound and differs from the closed conformation in the presence of an obtuse angle between the β3 subunit hybrid and I-like domains. We tested the hypothesis that opening of the hybrid-I-like domain interface increases ligand-binding affinity by mutationally …

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Bacillus Anthracis Virulence In Guinea Pigs Vaccinated With Anthrax Vaccine Adsorbed Is Linked To Plasmid Quantities And Clonality, Pamala R. Coker, Kimothy L. Smith, Patricia F. Fellows, Galena Rybachuck, Konstantin G. Kousoulas, Martin E. Hugh-Jones Mar 2003

Bacillus Anthracis Virulence In Guinea Pigs Vaccinated With Anthrax Vaccine Adsorbed Is Linked To Plasmid Quantities And Clonality, Pamala R. Coker, Kimothy L. Smith, Patricia F. Fellows, Galena Rybachuck, Konstantin G. Kousoulas, Martin E. Hugh-Jones

Faculty Publications

Bacillus anthracis is a bacterial pathogen of great importance, both historically and in the present. This study presents data collected from several investigations and indicates that B. anthracis virulence is associated with the clonality and virulence of plasmids pXO1 and pXO2. Guinea pigs vaccinated with Anthrax Vaccine Adsorbed were challenged with 20 B. anthracis isolates representative of worldwide genetic diversity. These same isolates were characterized with respect to plasmid copy number by using a novel method of quantitative PCR developed for rapid and efficient detection of B. anthracis from environmental samples. We found that the copy numbers for both pXO1 …

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Human Population Genetic Structure And Inference Of Group Membership, Michael J. Bamshad, Stephen Wooding, W. Scott Watkins, Christopher T. Ostler, Mark A. Batzer, Lynn B. Jorde Mar 2003

Human Population Genetic Structure And Inference Of Group Membership, Michael J. Bamshad, Stephen Wooding, W. Scott Watkins, Christopher T. Ostler, Mark A. Batzer, Lynn B. Jorde

Faculty Publications

A major goal of biomedical research is to develop the capability to provide highly personalized health care. To do so, it is necessary to understand the distribution of interindividual genetic variation at loci underlying physical characteristics, disease susceptibility, and response to treatment. Variation at these loci commonly exhibits geographic structuring and may contribute to phenotypic differences between groups. Thus, in some situations, it may be important to consider these groups separately. Membership in these groups is commonly inferred by use of a proxy such as place-of-origin or ethnic affiliation. These inferences are frequently weakened, however, by use of surrogates, such …

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Preparation And Identification Of Anti-Transforming Growth Factor Β1 U1 Small Nuclear Rna Chimeric Ribozyme In Vitro, Ju Sheng Lin, Yu Hu Song, Xin Juan Kong, Bin Li, Nan Zhi Liu, Xiao Li Wu, You Xin Jin Mar 2003

Preparation And Identification Of Anti-Transforming Growth Factor Β1 U1 Small Nuclear Rna Chimeric Ribozyme In Vitro, Ju Sheng Lin, Yu Hu Song, Xin Juan Kong, Bin Li, Nan Zhi Liu, Xiao Li Wu, You Xin Jin

Faculty Publications

Aim: To study the preparation and cleavage activity of anti-transforming growth factor (TGF)β1 U1 small nuclear (sn) RNA chimeric hammerhead ribozymes in vitro. Methods: TGFβ1 partial gene fragment was cloned into T-vector at the downstream of T7 promoter. 32p-labeled TGFβ1 partial transcripts as target RNA were transcribed in vitro and purified by denaturing polyacrylamide gel electrophoresis (PAGE). Anti-TGFβ1 ribozymes were designed by computer, then synthetic ribozyme fragments were cloned into the U1 ribozyme vector pZeoU1EcoSpe containing U1 snRNA promoter/enhancer and terminator. 32p-labeled U1 snRNA chimeric ribozyme transcripts were gel-purified, incubated with target-RNAs at different conditions and autoradiographed after running denaturing …

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Blue Crab Larval Dispersion And Retention In The Mississippi Bight: Testing The Hypothesis, Harriet M. Perry, Donald R. Johnson, Kirsten M. Larsen, Christine Trigg, Fred Vukovich Mar 2003

Blue Crab Larval Dispersion And Retention In The Mississippi Bight: Testing The Hypothesis, Harriet M. Perry, Donald R. Johnson, Kirsten M. Larsen, Christine Trigg, Fred Vukovich

Faculty Publications

An hypothesis relating physical forcing to dispersion and retention of blue crab larvae was tested in the area of the Mississippi Bight. Seasonal circulation patterns derived from a 3-dimensional, primitive equation, sigma-coordinate model of the Gulf of Mexico (GOM) indicate favorable conditions for offshore dispersal of larvae and their return to nearshore waters as megalopae occur between April and October. Large basin-scale events, such as Loop Current intrusions into the GOM with spin-off eddy generation and anomalies in average wind stress may interrupt this circulation pattern and change the settlement success rate. Meteorological and hydrological factors thought to influence settlement …

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Biochemical Composition Of Embryonic Blue Crabs Callinectes Sapidus Rathbun 1896 (Crustacea : Decapoda) From The Gulf Of Mexico, Janet R. Jacobs, Patricia M. Biesiot, Harriet M. Perry, Christine Trigg Mar 2003

Biochemical Composition Of Embryonic Blue Crabs Callinectes Sapidus Rathbun 1896 (Crustacea : Decapoda) From The Gulf Of Mexico, Janet R. Jacobs, Patricia M. Biesiot, Harriet M. Perry, Christine Trigg

Faculty Publications

Blue crab Callinectes sapidus embryos from the Mississippi Sound were sampled in spring and in late summer to determine patterns of biochemical composition and of yolk utilization during embryogenesis and to ascertain potential seasonal differences in biochemical composition. The diameter of spring embryos was similar to 6% greater than summer embryos but this significant size difference was due to increased water content, not to increased organic content. The general trend in initial biochemical composition was similar in both seasons; protein was the primary component at similar to 50% of initial dry weight followed by lipid (similar to 30%), ash (similar …

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