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Impact Of Reproductive Effort On Survival Of Rio Grande Wild Turkey Meleagris Gallop Intermedia Hens In Texas, Bret A. Collier, Kyle B. Melton, Jason B. Hardin, Nova J. Silvy, Markus J. Peterson Dec 2009

Impact Of Reproductive Effort On Survival Of Rio Grande Wild Turkey Meleagris Gallop Intermedia Hens In Texas, Bret A. Collier, Kyle B. Melton, Jason B. Hardin, Nova J. Silvy, Markus J. Peterson

Faculty Publications

The southeastern portion of the Edwards Plateau of Texas, historically a stronghold of Rio Grande wild turkeys Meleagris gallopavo intermedia, has seen the numbers of turkeys declining since the 1970s. Because hen survival is a key parameter affecting turkey population dynamics, we monitored radio-tagged Rio Grande wild turkey hens on the Edwards Plateau during 2001-2007 to compare survival during the breeding season in areas where turkey abundance has declined vs areas with stable populations. Breeding season survival was best predicted by a model that allowed variation during different periods of the reproductive season (initial nesting during 25 March 21 April, …


Existing Biomass Infrastructure And Theoretical Potential Biomass Production In The U.S, Jeremy R. Porter, Frank M. Howell, Philip B. Mason, Troy C. Blanchard Dec 2009

Existing Biomass Infrastructure And Theoretical Potential Biomass Production In The U.S, Jeremy R. Porter, Frank M. Howell, Philip B. Mason, Troy C. Blanchard

Faculty Publications

The recent and projected status of energy production and consumption in the U.S., resulting in substantial dependencies upon foreign oil, has continued to provide pressure on domestic energy security. All told, bio-energy systems, and biomass crop production in particular, will be important elements of national security, economic vitality, and public policy. Using biomass crop estimates based upon models developed at the Oak Ridge National Laboratory, we identify potential biomass crop production zones within the contiguous 48 states of the United States using spatially centered analytic methods. The Midwest and the South regions of the United States are, by far, the …


How Values In Scientific Discovery And Pursuit Alter Theory Appraisal, Kevin C. Elliott, Daniel J. Mckaughan Dec 2009

How Values In Scientific Discovery And Pursuit Alter Theory Appraisal, Kevin C. Elliott, Daniel J. Mckaughan

Faculty Publications

Philosophers of science readily acknowledge that nonepistemic values influence the discovery and pursuit of scientific theories, but many tend to regard these influences as epistemically uninteresting. The present paper challenges this position by identifying three avenues through which nonepistemic values associated with discovery and pursuit in contemporary pollution research influence theory appraisal: (1) by guiding the choice
of questions and research projects, (2) by altering experimental design, and (3) by affecting the creation and further investigation of theories or hypotheses. This analysis indicates that the effects of these values are sufficiently complex and epistemically significant to merit further attention.


Heterologous Expression Studies Of Saccharomyces Cerevisiae Reveal Two Distinct Trypanosomatid Caax Protease Activities And Identify Their Potential Targets, David Z. Mokry, Surya P. Manandhar, Kristen A. Chicola, George M. Santangelo, Walter K. Schmidt Dec 2009

Heterologous Expression Studies Of Saccharomyces Cerevisiae Reveal Two Distinct Trypanosomatid Caax Protease Activities And Identify Their Potential Targets, David Z. Mokry, Surya P. Manandhar, Kristen A. Chicola, George M. Santangelo, Walter K. Schmidt

Faculty Publications

The CaaX tetrapeptide motif typically directs three sequential posttranslational modifications, namely, isoprenylation, proteolysis, and carboxyl methylation. In all eukaryotic systems evaluated to date, two CaaX proteases (Rce1 and Ste24/Afc1) have been identified. Although the Trypanosoma brucei genome also encodes two putative CaaX proteases, the lack of detectable T. brucei Ste24 activity in trypanosome cell extracts has suggested that CaaX proteolytic activity within this organism is solely attributed to T. brucei Rce1 (J. R. Gillespie et al., Mol. Biochem. Parasitol. 153: 115-124. 2007). In this study, we demonstrate that both T. brucei Rce1 and T. brucei Ste24 are enzymatically active when …


Cellular Biophysics During Freezing Of Rat And Mouse Sperm Predicts Post-Thaw Motility, Mie Hagiwara, Jeung Hwan Choi, Ramachandra V. Devireddy, Kenneth P. Roberts, Willem F. Wolkers, Antoine Makhlouf, John C. Bischof Dec 2009

Cellular Biophysics During Freezing Of Rat And Mouse Sperm Predicts Post-Thaw Motility, Mie Hagiwara, Jeung Hwan Choi, Ramachandra V. Devireddy, Kenneth P. Roberts, Willem F. Wolkers, Antoine Makhlouf, John C. Bischof

Faculty Publications

Though cryopreservation of mouse sperm yields good survival and motility after thawing, cryopreservation of rat sperm remains a challenge. This study was designed to evaluate the biophysics (membrane permeability) of rat in comparison to mouse to better understand the cooling rate response that contributes to cryopreservation success or failure in these two sperm types. In order to extract subzero membrane hydraulic permeability in the presence of ice, a differential scanning calorimeter (DSC) method was used. By analyzing rat and mouse sperm frozen at 5°C/min and 20°C/min, heat release signatures characteristic of each sperm type were obtained and correlated to cellular …


Space-Efficient Framework For Top-K String Retrieval Problems, Wing Kai Hon, Rahul Shah, Jeffrey Scott Vitter Dec 2009

Space-Efficient Framework For Top-K String Retrieval Problems, Wing Kai Hon, Rahul Shah, Jeffrey Scott Vitter

Faculty Publications

Given a set D = {d1,d2, ⋯, dD} of D strings of total length n, our task is to report the "most relevant" strings for a given query pattern P. This involves somewhat more advanced query functionality than the usual pattern matching, as some notion of "most relevant" is involved. In information retrieval literature, this task is best achieved by using inverted indexes. However, inverted indexes work only for some predefined set of patterns. In the pattern matching community, the most popular pattern-matching data structures are suffix trees and suffix arrays. However, a typical suffix tree search involves going through …


Nanophotothermolysis Of Multiple Scattered Cancer Cells With Carbon Nanotubes Guided By Time-Resolved Infrared Thermal Imaging, Alexandru S. Biris, Dorin Boldor, Jason Palmer, William T. Monroe, Meena Mahmood, Enkeleda Dervishi, Yang Xu, Zhongrui Li, Ekaterina I. Galanzha, Vladimir P. Zharov Dec 2009

Nanophotothermolysis Of Multiple Scattered Cancer Cells With Carbon Nanotubes Guided By Time-Resolved Infrared Thermal Imaging, Alexandru S. Biris, Dorin Boldor, Jason Palmer, William T. Monroe, Meena Mahmood, Enkeleda Dervishi, Yang Xu, Zhongrui Li, Ekaterina I. Galanzha, Vladimir P. Zharov

Faculty Publications

Nanophotothermolysis with long laser pulses for treatment of scattered cancer cells and their clusters is introduced with the main focus on real-time monitoring of temperature dynamics inside and around individual cancer cells labeled with carbon nanotubes. This technique utilizes advanced time- and spatially-resolved thermal radiometry imaging for the visualization of laser-induced temperature distribution in multiple-point absorbing targets. The capability of this approach was demonstrated for monitoring of thermal effects under long laser exposure (from millisecond to seconds, wavelength 1064nm, maximum power 1W) of cervical cancer HeLa cells labeled with carbon nanotubes in vitro. The applications are discussed with a focus …


Water Uptake Threshold Of Rabbiteye (Vaccinium Ashei) Blueberries And Its Influence On Fruit Splitting, Donna A. Marshall-Shaw, James M. Spiers, Kenneth J. Curry Dec 2009

Water Uptake Threshold Of Rabbiteye (Vaccinium Ashei) Blueberries And Its Influence On Fruit Splitting, Donna A. Marshall-Shaw, James M. Spiers, Kenneth J. Curry

Faculty Publications

Split-resistant and split-susceptible rabbiteye blueberry fruit were evaluated at all stages of development to determine ''water uptake thresholds'' by soaking in distilled water. Weight increase after soaking was measured, and percent weight gain was calculated to take into consideration the weight increase of the fruit from development. The ratio of percent increase in volume to weight increase resulting from water uptake was calculated. Ratios of percent water uptake to weight increase between splitsusceptible 'Tifblue' and split-resistant 'Premier' blueberries were found to be similar. The split-susceptible 'Tifblue' had a 1.6 g/50 fruit increase with a 1.7% water uptake and a ratio …


Circadian Disruption, Per3, And Human Cytokine Secretion, Jaclyn Guess, James B. Burch, Kisito Ogoussan, Cheryl A. Armstead, Hongmei Zhang, Sara Wagner, James R. Hébert, Patricia Wood, Shawn D. Youngstedt, Lorne J. Hofseth, Udai P. Singh, Dawen Xie, William J. M. Hrushesky Dec 2009

Circadian Disruption, Per3, And Human Cytokine Secretion, Jaclyn Guess, James B. Burch, Kisito Ogoussan, Cheryl A. Armstead, Hongmei Zhang, Sara Wagner, James R. Hébert, Patricia Wood, Shawn D. Youngstedt, Lorne J. Hofseth, Udai P. Singh, Dawen Xie, William J. M. Hrushesky

Faculty Publications

Circadian disruption has been linked with inflammation, an established cancer risk factor. Per3 clock gene polymorphisms have also been associated with circadian disruption and with increased cancer risk. Patients completed a questionnaire and provided a blood sample prior to undergoing a colonoscopy (n=70). Adjusted mean serum cytokine concentrations (IL-6, TNF-alpha, gamma-INF, IL-I ra, IL-I-beta, VEGF) were compared among patients with high and low scores for fatigue (Multidimensional Fatigue Inventory), depressive symptoms (Beck Depression Inventory II), or sleep disruption (Pittsburgh Sleep Quality Index), or among patients with different Per3 clock gene variants. Poor sleep was associated with elevated VEGF, and fatigue-related …


Non-Negative Steady State Solutions To An Elliptic Biological Model, Brian Ibanez, Joon Hyuk Kang, Jungho Lee Dec 2009

Non-Negative Steady State Solutions To An Elliptic Biological Model, Brian Ibanez, Joon Hyuk Kang, Jungho Lee

Faculty Publications

The non-existence and existence of positive solutions for the generalized predator-prey biological model for two species of animals Δu + ug(u,v) = 0 in Ω, Δv + vh(u,v) = 0 in Ω, u = v = 0 on ∂Ω, is investigated in this paper. The techniques used in this paper are from elliptic theory, the upper-lower solution method, the maximum principles and spectrum estimates. The arguments also rely on detailed properties of solutions to logistic equations. © 2009 Academic Publications.


The Biogeochemical Influence Of Nitrate, Dissolved Oxygen, And Dissolved Organic Carbon On Stream Nitrate Uptake, Joseph A. Thouin, Wilfred M. Wollheim, Charles J. Vorosmarty, Jennifer M. Jacobs, William H. Mcdowell Dec 2009

The Biogeochemical Influence Of Nitrate, Dissolved Oxygen, And Dissolved Organic Carbon On Stream Nitrate Uptake, Joseph A. Thouin, Wilfred M. Wollheim, Charles J. Vorosmarty, Jennifer M. Jacobs, William H. Mcdowell

Faculty Publications

Streams are potential hotspots for retention and removal of NO3−, and understanding the mechanisms that enhance NO3− reactivity in stream systems is critical for predicting and preventing eutrophication. Both dissolved organic C (DOC) and dissolved O2 (DO) influence NO3− removal processes. Assessing the individual impacts of NO3−, DO, and DOC concentrations on stream NO3− removal is difficult because these factors covary and are coupled through the C and N cycles. We used an experimental approach to quantify the influences of NO3−, DOC, and DO on NO3− transport in headwater streams of the Ipswich and Parker River watersheds (Massachusetts, USA) with …


Compact Trench-Based Silicon-On-Insulator Rib Waveguide Ring Resonator With Large Free Spectral Range, Seunghyun Kim, Gregory P. Nordin, Weisheng Hu, Yusheng Qian, Jiguo Song, Gregory L. Wojcik Dec 2009

Compact Trench-Based Silicon-On-Insulator Rib Waveguide Ring Resonator With Large Free Spectral Range, Seunghyun Kim, Gregory P. Nordin, Weisheng Hu, Yusheng Qian, Jiguo Song, Gregory L. Wojcik

Faculty Publications

We demonstrate a compact trench-based silicon-on-insulator (SOI) rib waveguide ring resonator comprised of trench-based bends and splitters. It has a perimeter of 50 μm and occupies an area of only 25×25 μm. The measured free spectral range (FSR) is 13.2 nm, which the largest reported for an SOI rib waveguide ring resonator. The measured FSR, full width at half maximum, and quality factor match reasonably well with analytical calculations. Further calculation shows that a FSR of 50.8 nm is achievable for an SOI rib waveguide ring resonator with a perimeter of 15 μm.


Using Artificial Neural Networks And Model Predictive Control To Optimize Acoustically Assisted Doxorubicin Release From Polymeric Micelles, Ghaleb A. Husseini, Farouq S. Mjalli, William G. Pitt, Nabil M. Abdel-Jabbar Dec 2009

Using Artificial Neural Networks And Model Predictive Control To Optimize Acoustically Assisted Doxorubicin Release From Polymeric Micelles, Ghaleb A. Husseini, Farouq S. Mjalli, William G. Pitt, Nabil M. Abdel-Jabbar

Faculty Publications

We have been developing a drug delivery system that uses Pluronic P105 micelles to sequester a chemotherapeutic drug - namely, Doxorubicin (Dox) - until it reaches the cancer site. Ultrasound is then applied to release the drug directly to the tumor and in the process minimize the adverse side effects of chemotherapy on non-tumor tissues. Here, we present an artificial neural network (ANN) model that attempts to model the dynamic release of Dox from P105 micelles under different ultrasonic power intensities at two frequencies. The developed ANN model is then utilized to optimize the ultrasound application to achieve a target …


Prospective Associations Between Objective Measures Of Physical Activity And Fat Mass In 12-14 Year Old Children: The Avon Longitudinal Study Of Parents And Children (Alspac), Chris J. Riddoch, Sam D. Leary, Andy R. Ness, Steven N. Blair, Kevin Deere, Calum Mattocks, Alex Griffiths, George Davey Smith, Kate Tilling Nov 2009

Prospective Associations Between Objective Measures Of Physical Activity And Fat Mass In 12-14 Year Old Children: The Avon Longitudinal Study Of Parents And Children (Alspac), Chris J. Riddoch, Sam D. Leary, Andy R. Ness, Steven N. Blair, Kevin Deere, Calum Mattocks, Alex Griffiths, George Davey Smith, Kate Tilling

Faculty Publications

Objective: To investigate associations between physical activity at age 12 and subsequent adiposity at age 14.

Design: Prospective birth cohort study with data collected between 2003 and 2007.

Setting: Original recruitment in 1991-2 of 14,541 pregnant women living in the former County of Avon (United Kingdom).

Participants: At age 12, 11,952 children were invited to attend the research clinic. Of these, 7159 attended, and 4150 (1964 boys, 2186 girls) provided sufficient data on exposure, outcome, and confounding variables.

Main outcome measure: Fat mass at age 14, measured by dual emission x ray absorptiometry, associated with physical activity at age 12, …


Alternative Mrna Splicing Produces A Novel Biologically Active Short Isoform Of Pgc-1Α, Yubin Zhang, Peter Huypens, Aaron W. Adamson, Ji Suk Chang, Tara M. Henagan, Anik Boudreau, Natalie R. Lenard, David Burk, Johannes Klein, Nina Perwitz, Jeho Shin, Mathias Fasshauer, Anastasia Kralli, Thomas W. Gettys Nov 2009

Alternative Mrna Splicing Produces A Novel Biologically Active Short Isoform Of Pgc-1Α, Yubin Zhang, Peter Huypens, Aaron W. Adamson, Ji Suk Chang, Tara M. Henagan, Anik Boudreau, Natalie R. Lenard, David Burk, Johannes Klein, Nina Perwitz, Jeho Shin, Mathias Fasshauer, Anastasia Kralli, Thomas W. Gettys

Faculty Publications

The transcriptional co-activator PGC-1α regulates functional plasticity in adipose tissue by linking sympathetic input to the transcriptional program of adaptive thermogenesis. We report here a novel truncated form of PGC-1α(NT-PGC-1α) produced by alternative 3′ splicing that introduces an in-frame stop codon into PGC-1α mRNA. The expressed protein includes the first 267 amino acids of PGC-1α and 3 additional amino acids from the splicing insert. NT-PGC-1α contains the transactivation and nuclear receptor interaction domains but is missing key domains involved in nuclear localization, interaction with other transcription factors, and protein degradation. Expression and subcellular localization of NT-PGC-1α are dynamically regulated in …


Ultrafast Vibrational Spectroscopy Of A Degenerate Mode Of Guanidinium Chloride, Dmitriy Yu Vorobyev, Chun Hung Kuo, Jian Xin Chen, Daniel G. Kuroda, J. Nathan Scott, Jane M. Vanderkooi, Robin M. Hochstrasser Nov 2009

Ultrafast Vibrational Spectroscopy Of A Degenerate Mode Of Guanidinium Chloride, Dmitriy Yu Vorobyev, Chun Hung Kuo, Jian Xin Chen, Daniel G. Kuroda, J. Nathan Scott, Jane M. Vanderkooi, Robin M. Hochstrasser

Faculty Publications

Nearly degenerate asymmetric stretches with perpendicular transition dipóle moments of the deuterated guanidinium cation (DGdm-) in D2O and D-glycerol/D2O mixtures at 1600 cm-1 were investigated by linear FTIR spectroscopy and polarization dependent femtosecond pump-probe spectroscopy. The vibrational coupling of the asymmetric stretches of guanidinium occurs within 0.5 ps and leads to fast decay of the anisotropy to a level of 0.1. A systematic study of the influence of the coherence transfer on pump-probe signals is given. Following this decay, the anisotropy decays with a time constant of 4.1 ps in D2O by rotational diffusion about an axis perpendicular to the …


The Yeast High Mobility Group Protein Hmo2, A Subunit Of The Chromatin-Remodeling Complex Ino80, Binds Dna Ends, Sreerupa Ray, Anne Grove Nov 2009

The Yeast High Mobility Group Protein Hmo2, A Subunit Of The Chromatin-Remodeling Complex Ino80, Binds Dna Ends, Sreerupa Ray, Anne Grove

Faculty Publications

DNA damage is a common hazard that all cells have to combat. Saccharomyces cerevisiae HMO2 is a high mobility group protein (HMGB) that is a component of the chromatin-remodeling complex INO80, which is involved in double strand break (DSB) repair. We show here using DNA end-joining and exonuclease protection assays that HMO2 binds preferentially to DNA ends. While HMO2 binds DNA with both blunt and cohesive ends, the sequence of a single stranded overhang significantly affects binding, supporting the conclusion that HMO2 recognizes features at DNA ends. Analysis of the effect of duplex length on the ability of HMO2 to …


Alu Repeats Increase Local Recombination Rates, David J. Witherspoon, W. Scott Watkins, Yuhua Zhang, Jinchuan Xing, Whitney L. Tolpinrud, Dale J. Hedges, Mark A. Batzer, Lynn B. Jorde Nov 2009

Alu Repeats Increase Local Recombination Rates, David J. Witherspoon, W. Scott Watkins, Yuhua Zhang, Jinchuan Xing, Whitney L. Tolpinrud, Dale J. Hedges, Mark A. Batzer, Lynn B. Jorde

Faculty Publications

Background: Recombination rates vary widely across the human genome, but little of that variation is correlated with known DNA sequence features. The genome contains more than one million Alu mobile element insertions, and these insertions have been implicated in non-homologous recombination, modulation of DNA methylation, and transcriptional regulation. If individual Alu insertions have even modest effects on local recombination rates, they could collectively have a significant impact on the pattern of linkage disequilibrium in the human genome and on the evolution of the Alu family itself. Results: We carried out sequencing, SNP identification, and SNP genotyping around 19 AluY insertion …


3d-Surfer: Software For High-Throughput Protein Surface Comparison And Analysis, David La, Juan Esquivel-Rodríguez, Vishwesh Venkatraman, Bin Li, Lee Sael, Stephen Ueng, Steven Ahrendt, Daisuke Kihara Nov 2009

3d-Surfer: Software For High-Throughput Protein Surface Comparison And Analysis, David La, Juan Esquivel-Rodríguez, Vishwesh Venkatraman, Bin Li, Lee Sael, Stephen Ueng, Steven Ahrendt, Daisuke Kihara

Faculty Publications

Summary: We present 3D-SURFER, a web-based tool designed to facilitate high-throughput comparison and characterization of proteins based on their surface shape. As each protein is effectively represented by a vector of 3D Zernike descriptors, comparison times for a query protein against the entire PDB take, on an average, only a couple of seconds. The web interface has been designed to be as interactive as possible with displays showing animated protein rotations, CATH codes and structural alignments using the CE program. In addition, geometrically interesting local features of the protein surface, such as pockets that often correspond to ligand binding sites …


A Novel Plant Virus With Unique Properties Infecting Japanese Holly Fern, Rodrigo A. Valverde, Sead Sabanadzovic Nov 2009

A Novel Plant Virus With Unique Properties Infecting Japanese Holly Fern, Rodrigo A. Valverde, Sead Sabanadzovic

Faculty Publications

A novel RNA virus with a bipartite genome has been found associated with an emerging disease affecting Japanese holly fern (Cyrtomium falcatum). Diseased Japanese holly fern plants showed a variety of foliar symptoms and reduction in size. The virus was transmitted by grafting, as well as through spores from an infected plant. Partially purified preparations of the virus from infected ferns contained quasi-spherical particles that ranged from 30 to 40 nm in diameter. Doublestranded RNA (dsRNA) analyses from diseased plants yielded two major molecules of approximately 6.2 and 3.0 kbp in size, together with three other dsRNAs ascertained to be …


Novel Begomovirus Species Of Recombinant Nature In Sweet Potato (Ipomoea Batatas) And Ipomoea Indica: Taxonomic And Phylogenetic Implications, Gloria Lozano, Helena P. Trenado, Rodrigo A. Valverde, Jesús Navas-Castillo Nov 2009

Novel Begomovirus Species Of Recombinant Nature In Sweet Potato (Ipomoea Batatas) And Ipomoea Indica: Taxonomic And Phylogenetic Implications, Gloria Lozano, Helena P. Trenado, Rodrigo A. Valverde, Jesús Navas-Castillo

Faculty Publications

Viral diseases occur wherever sweet potato (Ipomoea batatas) is cultivated and because this crop is vegetatively propagated, accumulation and perpetuation of viruses can become a major constraint for production. Up to 90% reductions in yield have been reported in association with viral infections. About 20 officially accepted or tentative virus species have been found in sweet potato and other Ipomoea species. They include three species of begomoviruses (genus Begomovirus, family Geminiviridae) whose genomes have been fully sequenced. In this investigation, we conducted a search for begomoviruses infecting sweet potato and Ipomoea indica in Spain and characterized the complete genome of …


Morphological Evolution: By Any Means Necessary?, John C. Larkin Nov 2009

Morphological Evolution: By Any Means Necessary?, John C. Larkin

Faculty Publications

Recent debate has focused on the role of cis-regulatory mutations in the evolution of genes controlling morphology. Identification of the molecular basis of naturally occurring variation in leaf hair (trichome) density in Arabidopsis, combined with earlier work in the same system, sheds light on this debate. © 2009 Elsevier Ltd. All rights reserved.


6-Azahemiporphycene: A New Member Of The Porphyrinoid Family, Federica Mandoj, Sara Nardis, Giuseppe Pomarico, Manuela Stefanelli, Luca Schiaffino, Gianfranco Ercolani, Luca Prodi, Damiano Genovese, Nelsi Zaccheroni, Frank R. Fronczek, Kevin M. Smith, Xiao Xiao, Jing Shen, Karl M. Kadish, Roberto Paolesse Nov 2009

6-Azahemiporphycene: A New Member Of The Porphyrinoid Family, Federica Mandoj, Sara Nardis, Giuseppe Pomarico, Manuela Stefanelli, Luca Schiaffino, Gianfranco Ercolani, Luca Prodi, Damiano Genovese, Nelsi Zaccheroni, Frank R. Fronczek, Kevin M. Smith, Xiao Xiao, Jing Shen, Karl M. Kadish, Roberto Paolesse

Faculty Publications

The reaction of 5,10,15-triarylcorrole with 4-amino-4H-1,2,4-triazole provides another example of corroie ring expansion to give the corresponding 6-azahemiporphycene, a novel porphyrin analogue. The facile oxidation of the corroie ring is a required step for the ring expansion and for this reason the reaction fails in the case of corroles bearing meso-phenyl groups carrying electron-withdrawing substituents. Sterlc requirements also limited the scope of the reaction, which is not successful In the case of 2,6-disubstituted meso-aryl corroles. The occurrence of an initial oxidation is further supported by formation of the 6-azahemiporphycene derivative when the reaction is carried out under the same conditions, …


Comparative Acute Toxicity Of Neonicotinoid And Pyrethroid Insecticides To Non-Target Crayfish (Procambarus Clarkii) Associated With Rice-Crayfish Crop Rotations, Gary C. Barbee, Michael J. Stout Nov 2009

Comparative Acute Toxicity Of Neonicotinoid And Pyrethroid Insecticides To Non-Target Crayfish (Procambarus Clarkii) Associated With Rice-Crayfish Crop Rotations, Gary C. Barbee, Michael J. Stout

Faculty Publications

Background: Most insecticides used to control rice water weevil (Lissorhoptrus oryzophilus Kuscel) infestations are pyrethroids. However, pyrethroids are highly toxic to non-target crayfish associated with rice-crayfish crop rotations. One solution to the near-exclusive reliance on pyrethroids in a rice-crayfish pest management program is to incorporate neonicotinoid insecticides, which are insect specific and effective against weevils but not extremely toxic to crayfish. This study aimed to take the first step to assess neonicotinoids as alternatives to pyrethroids in rice-crayfish crop rotations by measuring the acute toxicities of three candidate neonicotinoid insecticides, clothianidin, dinotefuran and thiamethoxam, to juvenile Procambarus clarkii (Girard) crayfish …


Edwardsiella Ictaluri Encodes An Acid-Activated Urease That Is Required For Intracellular Replication In Channel Catfish (Ictalurus Punctatus) Macrophages, Natha J. Booth, Judith B. Beekman, Ronald L. Thune Nov 2009

Edwardsiella Ictaluri Encodes An Acid-Activated Urease That Is Required For Intracellular Replication In Channel Catfish (Ictalurus Punctatus) Macrophages, Natha J. Booth, Judith B. Beekman, Ronald L. Thune

Faculty Publications

Genomic analysis indicated that Edwardsiella ictaluri encodes a putative urease pathogenicity island containing the products of nine open reading frames, including urea and ammonium transporters. In vitro studies with wild-type E. ictaluri and a ureG::kan urease mutant strain indicated that E. ictaluri is significantly tolerant of acid conditions (pH 3.0) but that urease activity is not required for acid tolerance. Growth studies demonstrated that E. ictaluri is unable to grow at pH 5 in the absence of urea but is able to elevate the environmental pH from pH 5 to pH 7 and grow when exogenous urea is available. Substantial …


Arabidopsis Actin-Related Protein Arp5 In Multicellular Development And Dna Repair, Muthugapatti K. Kandasamy, Elizabeth C. Mckinney, Roger B. Deal, Aaron P. Smith, Richard B. Meagher Nov 2009

Arabidopsis Actin-Related Protein Arp5 In Multicellular Development And Dna Repair, Muthugapatti K. Kandasamy, Elizabeth C. Mckinney, Roger B. Deal, Aaron P. Smith, Richard B. Meagher

Faculty Publications

Actin-related protein 5 (ARP5) is a conserved subunit of the INO80 chromatin-remodeling complex in yeast and mammals. We have characterized the expression and subcellular distribution of Arabidopsis thaliana ARP5 and explored its role in the epigenetic control of multicellular development and DNA repair. ARP5-specific monoclonal antibodies localized ARP5 protein to the nucleoplasm of interphase cells in Arabidopsis and Nicotiana tabacum. ARP5 promoter-reporter fusions and the ARP5 protein are ubiquitously expressed. A null mutant and a severe knockdown allele produced moderately dwarfed plants with all organs smaller than the wild type. The small and slightly deformed organs such as leaves and …


Permanent Genetic Resources Added To Molecular Ecology Resources Database 1 May 2009-31 July 2009, Glenn R. Almany, Maurício P. De Arruda, Wolfgang Arthofer, Z. K. Atallah, Steven R. Beissinger, Michael L. Berumen, S. M. Bogdanowicz, S. D. Brown, Michael W. Bruford, C. Burdine, Jeremiah W. Busch, Nathan R. Campbell, D. Carey, Bryan C. Carstens, K. H. Chu, Marc A. Cubeta, J. P. Cuda, Zhaoxia Cui, L. E. Datnoff, J. A. Dávila, Emily S. Davis, R. M. Davis, Onno E. Diekmann, Eduardo Eizirik, J. A. Fargallo, Fabiano Fernandes, Hideo Fukuda, L. R. Gale, Elizabeth Gallagher, Yongqiang Gao, Philippe Girard, Anna Godhe, Evonnildo C. Gonçalves Nov 2009

Permanent Genetic Resources Added To Molecular Ecology Resources Database 1 May 2009-31 July 2009, Glenn R. Almany, Maurício P. De Arruda, Wolfgang Arthofer, Z. K. Atallah, Steven R. Beissinger, Michael L. Berumen, S. M. Bogdanowicz, S. D. Brown, Michael W. Bruford, C. Burdine, Jeremiah W. Busch, Nathan R. Campbell, D. Carey, Bryan C. Carstens, K. H. Chu, Marc A. Cubeta, J. P. Cuda, Zhaoxia Cui, L. E. Datnoff, J. A. Dávila, Emily S. Davis, R. M. Davis, Onno E. Diekmann, Eduardo Eizirik, J. A. Fargallo, Fabiano Fernandes, Hideo Fukuda, L. R. Gale, Elizabeth Gallagher, Yongqiang Gao, Philippe Girard, Anna Godhe, Evonnildo C. Gonçalves

Faculty Publications

This article documents the addition of 512 microsatellite marker loci and nine pairs of Single Nucleotide Polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Alcippe morrisonia morrisonia, Bashania fangiana, Bashania fargesii, Chaetodon vagabundus, Colletes floralis, Coluber constrictor flaviventris, Coptotermes gestroi, Crotophaga major, Cyprinella lutrensis, Danaus plexippus, Fagus grandifolia, Falco tinnunculus, Fletcherimyia fletcheri, Hydrilla verticillata, Laterallus jamaicensis coturniculus, Leavenworthia alabamica, Marmosops incanus, Miichthys miiuy, Nasua nasua, Noturus exilis, Odontesthes bonariensis, Quadrula fragosa, Pinctada maxima, Pseudaletia separata, Pseudoperonospora cubensis, Podocarpus elatus, Portunus trituberculatus, Rhagoletis cerasi, Rhinella schneideri, Sarracenia alata, Skeletonema marinoi, Sminthurus viridis, …


Reading Between The Lines To See Into The Past, David A. Ray, Roy N. Platt, Mark A. Batzer Nov 2009

Reading Between The Lines To See Into The Past, David A. Ray, Roy N. Platt, Mark A. Batzer

Faculty Publications

Transposable elements (TEs) are an important source of genome diversity and play a crucial role in genome evolution. A recent study by Zhao et al. describes novel patterns of TE diversification in the genome of the extinct mammoth Mammuthus primigenius. Analysis of Mammuthus has provided a unique genome landscape, a pivotal species for understanding TEs and genome evolution and hints at the diversity we verge on discovering by expanding our taxonomic sampling among genomes. Strategies based on this work might also revolutionize investigations of the interface between TE dynamics and genome diversity. © 2009 Elsevier Ltd. All rights reserved.


5́-Transducing Sva Retrotransposon Groups Spread Efficiently Throughout The Human Genome, Annette Damert, Julija Raiz, Axel V. Horn, Johannes Löwer, Hui Wang, Jinchuan Xing, Mark A. Batzer, Roswitha Löwer, Gerald G. Schumann Nov 2009

5́-Transducing Sva Retrotransposon Groups Spread Efficiently Throughout The Human Genome, Annette Damert, Julija Raiz, Axel V. Horn, Johannes Löwer, Hui Wang, Jinchuan Xing, Mark A. Batzer, Roswitha Löwer, Gerald G. Schumann

Faculty Publications

SVA elements represent the youngest family of hominid non-LTR retrotransposons, which alter the human genome continuously. They stand out due to their organization as composite repetitive elements. To draw conclusions on the assembly process that led to the current organization of SVA elements and on their transcriptional regulation, we initiated our study by assessing differences in structures of the 116 SVA elements located on human chromosome 19. We classified SVA elements into seven structural variants, including novel variants like 3′-truncated elements and elements with 5′-flanking sequence transductions. We established a genome-wide inventory of 5′-transduced SVA elements encompassing ∼8% of all …


Timing Of Migration And Patterns Of Winter Settlement By Henslow's Sparrows, Erik I. Johnson, Jennifer K. Dimiceli, Philip C. Stouffer Nov 2009

Timing Of Migration And Patterns Of Winter Settlement By Henslow's Sparrows, Erik I. Johnson, Jennifer K. Dimiceli, Philip C. Stouffer

Faculty Publications

Fall arrival is an important period in the life history of migratory birds because processes during this period determine where birds spend the winter, which is linked to subsequent survival and condition at the time of spring migration. Henslow's Sparrows (Ammodramus henslowii) winter in savannas of longleaf pine (Pinus palustris), but their secretive behavior limits our knowledge of their winter ecology, including behavioral processes that lead to their documented preference of an ephemeral habitat-recently burned savannas. We expected that upon arrival Henslow's Sparrows actively seek out recently burned savannas and that their over-winter location is dependent on intraspecific interactions during …