Digital Commons Network^™

Reproductive Status Regulates Expression Of Sex Steroid And Gnrh Receptors In The Olfactory Bulb, Karen P. Maruska, Russell D. Fernald

Faculty Publications

Neuromodulators including gonadotropin-releasing hormone (GnRH) and sex steroids help integrate an animal's internal physiological state with incoming external cues, and can have profound effects on the processing of behaviorally relevant information, particularly from the olfactory system. While GnRH and steroid receptors are present in olfactory processing regions across vertebrates, little is known about whether their expression levels change with internal physiological state or external social cues. We used qRT-PCR to measure mRNA levels of two GnRH receptors (GnRH-R1, GnRH-R2), five sex steroid receptors (estrogen receptors: ERα, ERβa, ERβb; androgen receptors: ARα, ARβ), and aromatase in the olfactory bulb of the …

A Membrane Protein/Signaling Protein Interaction Network For Arabidopsis Version Ampv2, Sylvie Lalonde, Antoinette Sero, Réjane Pratelli, Guillaume Pilot, Jin Chen, Maria I. Sardi, Saman A. Parsa, Do Young Kim, Biswa R. Acharya, Erica V. Stein, Heng Chen Hu, Florent Villiers, Kouji Takeda, Yingzhen Yang, Yong S. Han, Rainer Schwacke, William Chiang, Naohiro Kato, Dominique Loqué, Sarah M. Assmann, June M. Kwak, Julian I. Schroeder, Seung Y. Rhee, Wolf B. Frommer

Faculty Publications

Interactions between membrane proteins and the soluble fraction are essential for signal transduction and for regulating nutrient transport. To gain insights into the membrane-based interactome, 3,852 open reading frames (ORFs) out of a target list of 8,383 representing membrane and signaling proteins from Arabidopsis thaliana were cloned into a Gatewaycompatible vector. The mating-based split ubiquitin system was used to screen for potential protein-protein interactions (pPPIs) among 490 Arabidopsis ORFs. A binary robotic screen between 142 receptor-like kinases (RLKs), 72 transporters, 57 soluble protein kinases and phosphatases, 40 glycosyltransferases, 95 proteins of various functions, and 89 proteins with unknown function detected …

The Contribution Of Wildlife To Sustainable Natural Resource Utilization In Namibia: A Review, Diana L.Schalkwyk Van Schalkwyk, Kenneth W. Mcmillin, R. Corli Witthuhn, Louw C. Hoffman

Faculty Publications

Namibia is the driest country in sub-Saharan Africa, but well known for its richness in species and sustainable natural resource utilization. The Namibian farming sector consists mainly of extensive farming systems. Cattle production contributes 54% of the livestock sector's production output, followed by sheep and goats (25%), hides and skins (9%), and other forms of agricultural production (12%). Namibia's freehold farmers have obtained ownership rights over land and livestock since the early 1900s; commercial rights over wildlife and plants were given to freehold farmers in 1967 and to communal farmers in 1996. Natural resource-based production systems then overtook agricultural production …

Bb0250 Of Borrelia Burgdorferi Is A Conserved And Essential Inner Membrane Protein Required For Cell Division, Fang Ting Liang, Qilong Xu, Rakesh Sikdar, Ying Xiao, James S. Cox, William T. Doerrler

Faculty Publications

The gene bb0250 of Borrelia burgdorferi is a homolog of the dedA family, encoding integral inner membrane proteins that are present in nearly all species of bacteria. To date, no precise function has been attributed to any dedA gene. Unlike many bacterial species, such as Escherichia coli, which has eight dedA genes, B. burgdorferi possesses only one, annotated bb0250, providing a unique opportunity to investigate the functions of the dedA family. Here, we show that bb0250 is able to restore normal growth and cell division to a temperature-sensitive E. coli mutant with simultaneous deletions of two dedA genes, yqjA and …

Does Pyrogenicity Protect Burning Plants?, Paul R. Gagnon, Heather A. Passmore, William J. Platt, Jonathan A. Myers, C. E.Timothy Paine, Kyle E. Harms

Faculty Publications

Pyrogenic plants dominate many fire-prone ecosystems. Their prevalence suggests some advantage to their enhanced flammability, but researchers have had difficulty tying pyrogenicity to individual-level advantages. Based on our review, we propose that enhanced flammability in fire-prone ecosystems should protect the belowground organs and nearby propagules of certain individual plants during fires. We base this hypothesis on five points: (1) organs and propagules by which many fire-adapted plants survive fires are vulnerable to elevated soil temperatures during fires; (2) the degree to which burning plant fuels heat the soil depends mainly on residence times of fires and on fuel location relative …

Functional Traits And The Growth-Mortality Trade-Off In Tropical Trees, S. Joseph Wright, Kaoru Kitajima, Nathan J.B. Kraft, Peter B. Reich, Ian J. Wright, Daniel E. Bunker, Richard Condit, James W. Dalling, Stuart J. Davies, Sandra Díaz, Bettina M.J. Engelbrecht, Kyle E. Harms, Stephen P. Hubbell, Christian O. Marks, Maria C. Ruiz-Jaen, Cristina M. Salvador, Amy E. Zanne

Faculty Publications

A trade-off between growth and mortality rates characterizes tree species in closed canopy forests. This trade-off is maintained by inherent differences among species and spatial variation in light availability caused by canopy-opening disturbances. We evaluated conditions under which the trade-off is expressed and relationships with four key functional traits for 103 tree species from Barro Colorado Island, Panama. The trade-off is strongest for saplings for growth rates of the fastest growing individuals and mortality rates of the slowest growing individuals (r2 = 0.69), intermediate for saplings for average growth rates and overall mortality rates (r 2=0.46), and much weaker for …

Molecular Mechanisms Of Ligand-Mediated Attenuation Of Dna Binding By Marr Family Transcriptional Regulators, Inoka C. Perera, Anne Grove

Faculty Publications

Bacteria and archaea encode members of the large multiple antibiotic resistance regulator (MarR) family of transcriptional regulators. Generally, MarR homologs regulate activity of genes involved in antibiotic resistance, stress responses, virulence or catabolism of aromatic compounds. They constitute a diverse group of transcriptional regulators that includes both repressors and activators, and the conventional mode of regulation entails a genetic locus in which the MarR homolog and a gene under its regulation are encoded divergently; binding of the MarR homolog to the intergenic region typically represses transcription of both genes, while binding of a specific ligand to the transcription factor results …

Accurate Quantification Of Seasonal Rainfall And Associated Climate-Wildfire Relationships, Matthew G. Slocum, William J. Platt, Brian Beckage, Steve L. Orzell, Wayne Taylor

Faculty Publications

Wildfires are often governed by rapid changes in seasonal rainfall. Therefore, measuring seasonal rainfall on a temporally finescale should facilitate the prediction of wildfire regimes. To explore this hypothesis, daily rainfall data over a 58-yr period (1950-2007) in south-central Florida were transformed into cumulative rainfall anomalies (CRAs). This transformation allowed precise estimation of onset dates and durations of the dry and wet seasons, as well as a number of other variables characterizing seasonal rainfall. These variables were compared with parameters that describe ENSO and a wildfire regimeinthe region (at the Avon Park Air Force Range). Onset dates and durations were …

Colony Breeding System Influences Cuticular Bacterial Load Of Formosan Subterranean Termite (Isoptera: Rhinotermitidae) Workers, Claudia Husseneder, Dawn M. Simms, Gabriel K. Aluko, Jennifer Delatte

Faculty Publications

The goal of this study was to test whether the breeding system and/or the degree of inbreeding of field colonies of the Formosan subterranean termite, Coptotermes formosanus, Shiraki (Isoptera: Rhinotermitidae) influences bacterial load on the cuticle of foraging workers. We enumerated bacterial load on the cuticle of groups of workers foraging in 20 inground monitoring stations surrounding the French Market in New Orleans, LA, and identified bacteria species using 16S rRNA gene sequencing. We used microsatellite genotyping to assign the 20 worker groups to seven simple family colonies (headed by a single pair of reproductives) and four extended family colonies …

2-(2-Chloropyridin-3-Yl)-N-Ethyl-4-Methyl-1,3-Oxazole-5-Carboxamide, Guiqiu Yang, Jiakuang Liang, Haibo Yu, Bin Li

Faculty Publications

In the title compound, C12H12ClN3O 2, the dihedral angle between the aromatic rings is 8.42 (10)°. In the crystal, molecules are linked by N - H⋯O hydrogen bonds, generating C(4) chains propagating in [001].

Variation In The Checkpoint Kinase 2 Gene Is Associated With Type 2 Diabetes In Multiple Populations, Kari E. North, Nora Franceschini, Christy L. Avery, Lisa Baird, Mariaelisa Graff, Mark Leppert, Jay H. Chung, Jinghui Zhang, Craig Hanis, Eric Boerwinkle, Kelly A. Volcik, Megan L. Grove, Thomas H. Mosley, Charles Gu, Gerardo Heiss, James S. Pankow, David J. Couper, Christie M. Ballantyne, W. H. Linda Kao, Alan B. Weder, Richard S. Cooper, Georg B. Ehret, Ashley A. O'Connor, Aravinda Chakravarti, Steven C. Hunt

Faculty Publications

Identification and characterization of the genetic variants underlying type 2 diabetes susceptibility can provide important understanding of the etiology and pathogenesis of type 2 diabetes. We previously identified strong evidence of linkage for type 2 diabetes on chromosome 22 among 3,383 Hypertension Genetic Epidemiology Network (HyperGEN) participants from 1,124 families. The checkpoint 2 (CHEK2) gene, an important mediator of cellular responses to DNA damage, is located 0.22 Mb from this linkage peak. In this study, we tested the hypothesis that the CHEK2 gene contains one or more polymorphic variants that are associated with type 2 diabetes in HyperGEN individuals. In …

Interrogating Local Population Structure For Fine Mapping In Genome-Wide Association Studies, Huaizhen Qin, Nathan Morris, Sun J. Kang, Mingyao Li, Bamidele Tayo, Helen Lyon, Joel Hirschhorn, Richard S. Cooper, Xiaofeng Zhu

Faculty Publications

Motivation: Adjustment for population structure is necessary to avoid bias in genetic association studies of susceptibility variants for complex diseases. Population structure may differ from one genomic region to another due to the variability of individual ancestry associated with migration, random genetic drift or natural selection. Current association methods for correcting population stratification usually involve adjustment of global ancestry between study subjects.Results: We suggest interrogating local population structure for fine mapping to more accurately locate true casual genes by better adjusting the confounding effect due to local ancestry. By extensive simulations on genome-wide datasets, we show that adjusting global ancestry …

Discrete Diffusion Models To Study The Effects Of Mg2+ Concentration On The Phopq Signal Transduction System, Preetam Ghosh, Samik Ghosh, Kalyan Basu, Sajal K. Das, Chaoyang Zhang

Faculty Publications

Background: The challenge today is to develop a modeling and simulation paradigm that integrates structural, molecular and genetic data for a quantitative understanding of physiology and behavior of biological processes at multiple scales. This modeling method requires techniques that maintain a reasonable accuracy of the biological process and also reduces the computational overhead. This objective motivates the use of new methods that can transform the problem from energy and affinity based modeling to information theory based modeling. To achieve this, we transform all dynamics within the cell into a random event time, which is specified through an information domain …

A New Approach To Construct Pathway Connected Networks And Its Application In Dose Responsive Gene Expression Profiles Of Rat Liver Regulated By 2,4dnt, Sudhir Chowbina, Youping Deng, Junmei Ai, Xiaogang Wu, Xin Guan, Mitchell S. Wilbanks, Barbara Lynn Escalon, Edward J. Perkins, Jake Y. Chen

Faculty Publications

Background: Military and industrial activities have lead to reported release of 2,4-dinitrotoluene (2,4DNT) into soil, groundwater or surface water. It has been reported that 2,4DNT can induce toxic effects on humans and other organisms. However the mechanism of 2,4DNT induced toxicity is still unclear. Although a series of methods for gene network construction have been developed, few instances of applying such technology to generate pathway connected networks have been reported.

Results: Microarray analyses were conducted using liver tissue of rats collected 24h after exposure to a single oral gavage with one of five concentrations of 2,4DNT. We observed …

Measured And Estimated Ground Reaction Forces For Multi-Segment Foot Models, Dustin A. Bruening, Kevin M. Cooney, Frank L. Buczek

Faculty Publications

Accurate measurement of ground reaction forces under discrete areas of the foot is important in the development of more advanced foot models, which can improve our understanding of foot and ankle function. To overcome current equipment limitations, a few investigators have proposed combining a pressure mat with a single force platform and using a proportionality assumption to estimate subarea shear forces and free moments. In this study, two adjacent force platforms were used to evaluate the accuracy of the proportionality assumption on a three segment foot model during normal gait. Seventeen right feet were tested using a targeted walking approach, …

Quantitative Template For Subtyping Primary Progressive Aphasia, Marsel Mesulam, Christina Wieneke, Emily Rogalski, Derin J. Cobia, Cynthia Thompson, Sandra Weintraub

Faculty Publications

Objective—To provide a quantitative algorithm for classifying primary progressive aphasia (PPA) into agrammatic (PPA-G), semantic (PPA-S) and logopenic (PPA-L) variants, each of which is known to have a different probability of association with Alzheimer’s disease (AD) versus frontotemporal lobar degeneration (FTLD).

Design—Prospectively and consecutively enrolled 16 PPA patients tested with neuropsychological instruments and magnetic resonance imaging (MRI).

Setting—University medical center. Participants—PPA patients recruited nationally in the USA as part of a longitudinal study.

Results—A two-dimensional template, reflecting performance on tests of syntax (Northwestern Anagram Test) and lexical semantics (Peabody Picture Vocabulary Test), classified all 16 patients in concordance with a …

2k09 And Thereafter: The Coming Era Of Integrative Bioinformatics, Systems Biology And Intelligent Computing For Functional Genomics And Personalized Medicine Research, Jack Y. Yang, Andrzej Niemierko, Ruzena Bajcsy, Dong Xu, Brian D. Athey, Aidong Zhang, Okan K. Ersoy, Guo-Zheng Li, Mark Borodovsky, Joe C. Zhang, Hamid R. Arabnia, Youping Deng, A. Keith Dunker, Yunlong Liu, Arif Ghafoor

Faculty Publications

Significant interest exists in establishing synergistic research in bioinformatics, systems biology and intelligent computing. Supported by the United States National Science Foundation (NSF), International Society of Intelligent Biological Medicine (http://www.ISIBM.org), International Journal of Computational Biology and Drug Design (IJCBDD) and International Journal of Functional Informatics and Personalized Medicine, the ISIBM International Joint Conferences on Bioinformatics, Systems Biology and Intelligent Computing (ISIBM IJCBS 2009) attracted more than 300 papers and 400 researchers and medical doctors world-wide. It was the only inter/multidisciplinary conference aimed to promote synergistic research and education in bioinformatics, systems biology and intelligent computing. The conference committee …

Systematic Approach To Electrostatically Induced 2d Crystallization Of Nanoparticles At Liquid Interfaces, Sumit Kewalramani, Suntao Wang, Yuan Lin, Huong Giang Nguyen, Qian Wang, Masafumi Fukuto, Lin Yang

Faculty Publications

We report an experimental demonstration of a strategy for inducing two-dimensional (2D)crystallization of charged nanoparticles on oppositely charged fluid interfaces. This strategy aims to maximize the interfacial adsorption of nanoparticles, and hence their lateral packing density, by utilizing a combination of weakly charged particles and a high surface charge density on the planar interface. In order to test this approach, we investigated the assembly of cowpea mosaic virus (CPMV) on positively charged lipid monolayers at the aqueous solution surface, by means of in situX-ray scattering measurements at the liquid–vapor interface. Theassembly was studied as a function of the solution …

Cryomicroscopic Investigations Of Freezing Processes In Cell Suspensions, Tathagata Acharya, Ram V. Devireddy

Faculty Publications

This study evaluates the freezing response of three different cell types, Pacific oyster embryos (∼50 m in diameter), Jurkat cells and HeLa cells (∼12 to 15 m's in diameter), using cryomicroscopy. Freezing experiments were performed on oyster embryos at cooling rates of either 5 or 10 °C/min, while Jurkats were cooled at either 1 or 10 °C/min and HeLa cells were cooled at either 1, 15 or 20 °C/min, respectively. The experiments with oyster embryos were primarily designed to investigate the phenomena of intracellular ice formation (IIF) while the experiments for Jurkat and HeLa cells were designed to investigate both …

New Insights Into The Organization, Recombination, Expression And Functional Mechanism Of Low Molecular Weight Glutenin Subunit Genes In Bread Wheat, Lingli Dong, Xiaofei Zhang, Dongcheng Liu, Huajie Fan, Jiazhu Sun, Zhongjuan Zhang, Huanju Qin, Bin Li, Shanting Hao, Zhensheng Li, Daowen Wang, Aimin Zhang, Hong Qing Ling

Faculty Publications

The bread-making quality of wheat is strongly influenced by multiple low molecular weight glutenin subunit (LMW-GS) proteins expressed in the seeds. However, the organization, recombination and expression of LMW-GS genes and their functional mechanism in bread-making are not well understood. Here we report a systematic molecular analysis of LMW-GS genes located at the orthologous Glu-3 loci (Glu-A3, B3 and D3) of bread wheat using complementary approaches (genome wide characterization of gene members, expression profiling, proteomic analysis). Fourteen unique LMW-GS genes were identified for Xiaoyan 54 (with superior bread-making quality). Molecular mapping and recombination analyses revealed that the three Glu-3 loci …

In Vitro Gene Regulatory Networks Predict In Vivo Function Of Liver, Youping Deng, David R. Johnson, Junmei Ai, Edward J. Perkins, Sharon A. Meyer

Faculty Publications

Background: Evolution of toxicity testing is predicated upon using in vitro cell based systems to rapidly screen and predict how a chemical might cause toxicity to an organ in vivo. However, the degree to which we can extend in vitro results to in vivo activity and possible mechanisms of action remains to be fully addressed.

Results: Here we use the nitroaromatic 2,4,6-trinitrotoluene (TNT) as a model chemical to compare and determine how we might extrapolate from in vitro data to in vivo effects. We found 341 transcripts differentially expressed in common among in vitro and in vivo assays …

Nucleosome Structure Incorporated Histone Acetylation Site Prediction In Arabidopsis Thaliana, Chen Zhao, Hui Liu, Youping Deng, Tieliu Shu

Faculty Publications

Background

Acetylation is a crucial post-translational modification for histones, and plays a key role in gene expression regulation. Due to limited data and lack of a clear acetylation consensus sequence, a few researches have focused on prediction of lysine acetylation sites. Several systematic prediction studies have been conducted for human and yeast, but less for Arabidopsis thaliana.

Results

Concerning the insufficient observation on acetylation site, we analyzed contributions of the peptide-alignment-based distance definition and 3D structure factors in acetylation prediction. We found that traditional structure contributes little to acetylation site prediction. Identified acetylation sites of histones in Arabidopsis thaliana …

Global Protein Interactome Exploration Through Mining Genome-Scale Data In Arabidopsis Thaliana, Feng Xu, Guang Li, Chen Zhao, Yuhua Li, Peng Li, Jian Cui, Youping Deng, Tieliu Shi

Faculty Publications

Background

Many essential cellular processes, such as cellular metabolism, transport, cellular metabolism and most regulatory mechanisms, rely on physical interactions between proteins. Genome-wide protein interactome networks of yeast, human and several other animal organisms have already been established, but this kind of network reminds to be established in the field of plant.

Results

We first predicted the protein protein interaction in Arabidopsis thaliana with methods, including ortholog, SSBP, gene fusion, gene neighbor, phylogenetic profile, coexpression, protein domain, and used Naïve Bayesian approach next to integrate the results of these methods and text mining data to build a genome-wide protein interactome …

Speckle Reducing Bilateral Filter For Cattle Follicle Segmentation, Jinshang Teng, Shengwen Guo, Qingling Sun, Youping Deng, Dongfeng Zhou

Faculty Publications

Background: Ultrasound imaging technology has wide applications in cattle reproduction and has been used to monitor individual follicles and determine the patterns of follicular development. However, the speckles in ultrasound images affect the post-processing, such as follicle segmentation and finally affect the measurement of the follicles. In order to reduce the effect of speckles, a bilateral filter is developed in this paper.

Results: We develop a new bilateral filter for speckle reduction in ultrasound images for follicle segmentation and measurement. Different from the previous bilateral filters, the proposed bilateral filter uses normalized difference in the computation of the …

Generation, Analysis And Functional Annotation Of Expressed Sequence Tags From The Sheepshead Minnow (Cyprinodon Variegatus), Mehdi Pirooznia, Alexander Pozhitkov, Edward J. Perkins, Youping Deng, Marius Brouwer

Faculty Publications

Background: Sheepshead minnow (Cyprinodon variegatus) are small fish capable of withstanding exposure to very low levels of dissolved oxygen, as well as extreme temperatures and salinities. It is an important model in understanding the impacts and biological response to hypoxia and co-occurring compounding stressors such as polycyclic aromatic hydrocarbons, endocrine disrupting chemicals, metals and herbicides. Here, we initiated a project to sequence and analyze over 10,000 ESTs generated from the Sheepshead minnow (Cyprinodon variegatus) as a resource for investigating stressor responses. Results: We sequenced 10,858 EST clones using a normalized cDNA library made from larval, embryonic and adult suppression subtractive …

Genetic Diversity In A Deep-Sea Harpacticoid Copepod Found Near Two Oil-Drilling Sites In The Gulf Of Mexico, Christopher S. Gregg, David W. Foltz, John W. Fleeger

Faculty Publications

Sites adjacent to (near-field) and distant from (far-field) oil-drilling platforms were sampled for harpacticoid copepods at two locations in the northern Gulf of Mexico off the coast of Louisiana, both at depths of ≈ 1100 m. The sites were located at Garden Banks Area Block 602 (GB 602) and Mississippi Canyon Area Block 292 (MC 292). Near-field sites at both locations were characterized by large numbers of a single undescribed species of harpacticoid copepod, Bathycletopsyllus sp., but this species was essentially absent from far-field sites. We sequenced a 710 bp portion of the mitochondrial cytochrome c oxidase subunit I gene …

Lines And Sines Of Primate Evolution, Miriam K. Konkel, Jerilyn A. Walker, Mark A. Batzer

Faculty Publications

The primate order is a monophyletic group thought to have diverged from the Euarchonta more than 65 mya.1 Recent paleontological and molecular evolution studies place the last common ancestor of primates even earlier (≥ 85 mya).2 More than 300 extant primate species are recognized today,3, 4 clearly emphasizing their diversity and success. Our understanding of the evolution of primates and the composition of their genomes has been revolutionized within the last decade through the increasing availability and analyses of sequenced genomes. However, several aspects of primate evolution have yet to be resolved. DNA sequencing of a wider array of primate …

Development Of Microsatellites For Genetic Analyses And Population Assignment Of The Cat Flea (Siphonaptera: Pulicidae), Claudia Husseneder, Susan P. Garner, Lane D. Foil, Kevin R. Macaluso

Faculty Publications

Cat fleas, Ctenocephalides felis (Bouch) (Siphonaptera: Pulicidae), are common ectoparasites of companion animals that negatively impact their hosts directly by causing dermatitis and blood loss during feeding and indirectly through the potential transmission of disease causing agents. We isolated and characterized seven novel microsatellite loci from a partial genomic library of the cat flea enriched for di-, tri-, and tetranucleotide repeats. We screened these loci in cat fleas from two laboratory colonies and one wild-caught population collected at a temporary animal shelter (Parker coliseum) in Baton Rouge, LA. Six loci were polymorphic, with two to 15 alleles per locus and …

Genome Structures And Halophyte-Specific Gene Expression Of The Extremophile Thellungiella Parvula In Comparison With Thellungiella Salsuginea (Thellungiella Halophila) And Arabidopsis, Dong Ha Oh, Maheshi Dassanayake, Jeffrey S. Haas, Anna Kropornika, Chris Wright, Matilde Paino D'Urzo, Hyewon Hong, Shahjahan Ali, Alvaro Hernandez, Georgina M. Lambert, Gunsu Inan, David W. Galbraith, Ray A. Bressan, Dae Jin Yun, Jian Kang Zhu, John M. Cheeseman, Hans J. Bohnert

Faculty Publications

The genome of Thellungiella parvula, a halophytic relative of Arabidopsis (Arabidopsis thaliana), is being assembled using Roche-454 sequencing. Analyses of a 10-Mb scaffold revealed synteny with Arabidopsis, with recombination and inversion and an uneven distribution of repeat sequences. T. parvula genome structure and DNA sequences were compared with orthologous regions from Arabidopsis and publicly available bacterial artificial chromosome sequences from Thellungiella salsuginea (previously Thellungiella halophila). The three-way comparison of sequences, from one abiotic stress-sensitive species and two tolerant species, revealed extensive sequence conservation and microcolinearity, but grouping Thellungiella species separately from Arabidopsis. However, the T. parvula segments are distinguished from …

Assessment Of Glycoprotein Interactions With 4-[(2-Aminoethyl)Carbamoyl] Phenylboronic Acid Surfaces Using Surface Plasmon Resonance Spectroscopy, Jennifer Mac Alindong De Guzman, Steven A. Soper, Robin L. Mccarley

Faculty Publications

Reported here are analyses of the interactions between a select group of solution-phase glycoproteins and a unique boronic acid capture surface. The boronic acid derivative, 4-[(2-aminoethyl)carbamoyl]phenylboronic acid, AECPBA, was synthesized and then immobilized on carboxymethyl dextran surfaces using simple coupling methods. From surface plasmon resonance spectroscopy responses, it is found that model glycoproteins interact strongly with the AECPBA surface and subsequently can be readily released from the AECPBA surface using borate buffer. A striking difference between the glycoproteins fetuin and asialofetuin (desialylated fetuin), in terms of glycoprotein binding to the AECPBA surface, indicates that the interaction of glycoproteins with the …