Digital Commons Network^™

Variants In Cxadr And F2rl1 Are Associated With Blood Pressure And Obesity In African-Americans In Regions Identified Through Admixture Mapping, Priya B. Shetty, Hua Tang, Bamidele O. Tayo, Alanna C. Morrison, Craig L. Hanis, Dabeeru C. Rao, Jeffery H. Young, Ervin R. Fox, Eric Boerwinkle, Richard S. Cooper, Neil J. Risch, Xiaofeng Zhu

Faculty Publications

OBJECTIVE: Genetic variants in 296 genes in regions identified through admixture mapping of hypertension, BMI, and lipids were assessed for association with hypertension, blood pressure (BP), BMI, and high-density lipoprotein cholesterol (HDL-C). METHODS: This study identified coding SNPs identified from HapMap2 data that were located in genes on chromosomes 5, 6, 8, and 21, wherein ancestry association evidence for hypertension, BMI, or HDL-C was identified in previous admixture mapping studies. Genotyping was performed in 1733 unrelated African-Americans from the National Heart, Lung and Blood Institute's Family Blood Pressure Project, and gene-based association analyses were conducted for hypertension, SBP, DBP, BMI, …

Oyun: A New, Free Program For Iterated Prisoner’S Dilemma Tournaments In The Classroom, Charles H. Pence, Lara Buchak

Faculty Publications

Evolutionary applications of game theory present one of the most pedagogically accessible varieties of genuine, contemporary theoretical biology. We present here Oyun (oy-oon, http://charlespence.net/oyun), a program designed to run iterated prisoner's dilemma tournaments, competitions between prisoner's dilemma strategies developed by the students themselves. Using this software, students are able to readily design and tweak their own strategies, and to see how they fare both in round-robin tournaments and in “evolutionary” tournaments, where the scores in a given “generation” directly determine contribution to the population in the next generation. Oyun is freely available, runs on Windows, Mac, and Linux computers, …

The Extrinsic Proteins Of Photosystem Ii, Terry M. Bricker, Johnna L. Roose, Robert D. Fagerlund, Laurie K. Frankel, Julian J. Eaton-Rye

Faculty Publications

In this review we examine the structure and function of the extrinsic proteins of Photosystem II. These proteins include PsbO, present in all oxygenic organisms, the PsbP and PsbQ proteins, which are found in higher plants and eukaryotic algae, and the PsbU, PsbV, CyanoQ, and CyanoP proteins, which are found in the cyanobacteria. These proteins serve to optimize oxygen evolution at physiological calcium and chloride concentrations. They also shield the Mn 4CaO 5 cluster from exogenous reductants. Numerous biochemical, genetic and structural studies have been used to probe the structure and function of these proteins within the photosystem. We will …

Workshop And Panel Discussion: High-Throughput Cryopreservation Of Germplasm As An Exchange Currency For Genetic Resources, Zoltán M. Varga, Terrence R. Tiersch

Faculty Publications

No abstract provided.

Offspring Production With Cryopreserved Sperm From A Live-Bearing Fish Xiphophorus Maculatus And Implications For Female Fecundity, Huiping Yang, Markita G. Savage, Leona Hazlewood, Ronald B. Walter, Terrence R. Tiersch

Faculty Publications

Xiphophorus fishes are well-established models for biomedical research of spontaneous or induced tumors, and their use in research dates back to the 1930s. Currently, 58 well-pedigreed lines exist among 24 Xiphophorus species housed as live animals at the Xiphophorus Genetic Stock Center. The technique of sperm cryopreservation has been applied to preserve these valuable genetic resources, and production of offspring has been reported with cryopreserved sperm in two species (X. helleri and X. couchianus). The goal of this research was to establish protocols for sperm cryopreservation and artificial insemination that yield live young in X. maculatus, a widely used research …

Arbuscular Mycorrhizae Occur In Common Spartina Species, Angela K. Burcham, Joy H. Merino, Thomas C. Michot, John A. Nyman

Faculty Publications

We report the presence of arbuscular mycorrhizae (AM) in Spartina alterniflora Loisel roots. Roots were sampled for AM in field-collected and greenhouse-maintained Spartina patens (Aiton) Muhl. and S. alterniflora, the dominant species of Louisiana's brackish and saline marshes, respectively. Previous reports of AM association in these Spartina sp. are limited and conflicting. Field-collected 5. alterniflora had minimal AM (2.4%), whereas 39.5% of the 5. patens roots examined were AM colonized. Greenhouse conditions of reduced salinity [3 parts per thousand (ppt)] appeared to increase AM association for 5. patens compared with field samples. AM occurrence varied significantly among the three sample …

Characterization Of Lateral Root Development At The Onset Of Storage Root Initiation In 'Beauregard' Sweetpotato Adventitious Roots, Arthur Villordon, Don Labonte, Julio Solis, Nurit Firon

Faculty Publications

This study characterized lateral root (LR) development attributes during the onset of storage root (SR) initiation stage in 'Beauregard' sweetpotato. SR initiation has been defined as the appearance of cambia around the protoxylem and secondary xylem elements. Our results showed that 20-day-old adventitious roots (ARs) classified as SRs had 53% and 85% greater mean LR count than pencil roots (PRs) and lignified roots (LGs), respectively. SRs had 53% and 78% greater mean LR density relative to PRs and LGs, respectively. SRs had 66% and 130% greater mean total LR length than PRs and LGs, respectively. SRs had lower mean main …

Host Interferon-Γ Inducible Protein Contributes To Brucella Survival., Jennifer A. Ritchie, Adam Rupper, James A. Cardelli, Bryan H. Bellaire

Faculty Publications

Brucella spp. are highly adapted intracellular pathogens of mammals that cause chronic infections while surving and replicating in host monocytes and macrophages. Although monocytes are normally susceptible to infection, pretreatment with pro-inflammatory cytokine interferon-γ (IFN-γ) activates cellular defense mechanisms that increase intracellular killing of Brucella and prevents bacterial replication. We examined the contribution of the IFN-γ inducible GTPase, LRG-47, to B. abortus 2308 infection in in vitro and in vivo murine models. Infecting non-activated macrophages from LRG-47(-/-) mice revealed that loss of this host protein negatively effected the intracellular survival and replication of IgG opsonized B. abortus. In contrast, survival …

Overexpression Of Arabidopsis And Rice Stress Genes' Inducible Transcription Factor Confers Drought And Salinity Tolerance To Rice, Karabi Datta, Niranjan Baisakh, Moumita Ganguly, Sellapan Krishnan, Kazuko Yamaguchi Shinozaki, Swapan K. Datta

Faculty Publications

Rice yield is greatly affected by environmental stresses such as drought and salinity. In response to the challenge of producing rice plants tolerant to these stresses, we introduced cDNA encoding the transcription factors DREB1A and DREB1B under the control of the stress inducible rd29 promoter. Two different indica rice cultivars were used, BR29, an improved commercially cultivated variety from Bangladesh and IR68899B, an IRRI bred maintainer line for hybrid rice. Agrobacterium mediated transformation of BR29 was done independently with DREB1A isolated from rice and Arabidopsis and DREB1B isolated from rice, whereas biolistic transformation was done with rice- DREB1B in the …

The Origin And In Situ Identification Of Uncultured Gut Bacteria In Fourth-Instar Larvae Of The Red Imported Fire Ant, Solenopsis Invicta (Hymenoptera: Formicidae), Albert H. Lee, Linda Hooper-Bui

Faculty Publications

This study corroborated the findings of Lee et al. (2008) and relied on culture-independent techniques, particularly 16SrDNA sequencing. Results from the study demonstrated that ants in captivity completely change their gut bacterial diversity strongly supports the conclusion that fire ant gut bacteria are environmental in origin. Bacteria in the fourth-instar larvae were present because they were consumed with food sources. From molecular data, a differing list of bacteria from each colony with different branching and clustering among bacterial clades in the phylogenetic trees were found. Different bacterial diversity and evenness was found in all colonies, which provided evidence against the …

H2/Ch4 Ratios Cannot Reliably Distinguish Abiotic Vs. Biotic Methane In Natural Hydrothermal Systems, Susan Q. Lang, G. L. Früh-Green, D. S. Kelley, M. D. Lilley, G. Proskurowski, E. P. Reeves

Faculty Publications

No abstract provided.

Photoproduction Of Ammonium In The Southeastern Beaufort Sea And Its Biogeochemical Implications, H. Xie, S. Belanger, G. Song, Ronald Benner, A. Taalba, M. Blais, J. É. Tremblay, M. Babin

Faculty Publications

Photochemistry of dissolved organic matter (DOM) plays an important role in marine biogeochemical cycles, including the regeneration of inorganic nutrients. DOM photochemistry affects nitrogen cycling by converting bio-refractory dissolved organic nitrogen to labile inorganic nitrogen, mainly ammonium (NH₄⁺).During the August 2009 Mackenzie Light and Carbon (MALINA) Program, the absorbed photon-based efficiency spectra of NH₄⁺ photoproduction (i.e. photoammonification) were determined using water samples from the SE Beaufort Sea, including the Mackenzie River estuary, shelf, and Canada Basin. The photoammonification efficiency decreased with increasing wavelength across the ultraviolet and visible regimes and was higher in offshore waters …

Publish And Flourish, Stephen K. Taylor

Faculty Publications

At primarily undergraduate institutions (PUIs), teaching is usually the primary responsibility, and many PUI professors do not regularly produce publications, even if they were hired to include research as an important responsibility. Most instructors at these schools are busy enough with just teaching and other academic responsibilities. It can seem intimidating to think of doing research, writing a research proposal, then a publication, and so on. Perhaps the sum of these duties may seem like a daunting set of tasks, and a "fear factor," consciously or subconsciously, may slow or even stop the research initiative. But at PUIs, it is …

The Family And The Market -- Redux, Maxine Eichner

Faculty Publications

No abstract provided.

Piezoelectric Wafer Active Sensors In Lamb Wave-Based Structural Health Monitoring, Lingyu Yu, Victor Giurgiutiu

Faculty Publications

Recent advancements in sensors and information technologies have resulted in new methods for structural health monitoring (SHM) of the performance and deterioration of structures. The enabling element is the piezoelectric wafer active sensor (PWAS). This paper presents an introduction to PWAS transducers and their applications in Lamb wave-based SHM. We begin by reviewing the fundamentals of piezoelectric intelligent materials. Then, the mechanism of using PWAS transducers as Lamb wave transmitters and receivers is presented. PWAS interact with the host structure through the shear-lag model. Lamb wave mode tuning can be achieved by judicious combination of PWAS dimensions, frequency value, and …

Tracing The Evolution Of Chiropractic Students’ Confidence In Clinical And Patient Communication Skills During A Clinical Internship: A Multi-Methods Study, Mark Hecimovich, Simone Volet

Faculty Publications

Background: Anecdotal evidence points to variations in individual students’ evolving confidence in clinical and patient communication skills during a clinical internship. A better understanding of the specific aspects of internships that contribute to increasing or decreasing confidence is needed to best support students during the clinical component of their study.

Methods: A multi-method approach, combining two large-scale surveys with 269 students and three in-depth individual interviews with a sub-sample of 29 students, was used to investigate the evolution of change in student confidence during a 10-month long internship. Change in levels of confidence in patient communication and clinical skills was …

A Molecular Perspective On The Ageing Of Marine Dissolved Organic Matter, R. Flerus, O. J. Lechtenfeld, B. P. Koch, S. L. Mccallister, P. Schmitt-Kopplin, Ronald Benner, G. Kattner

Faculty Publications

Dissolved organic matter (DOM) was extracted by solid-phase extraction (SPE) from 137 water samples from different climate zones and different depths along an eastern Atlantic Ocean transect. The extracts were analyzed with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) with electrospray ionization (ESI). Δ14C analyses were performed on subsamples of the SPE-DOM. In addition, the amount of dissolved organic carbon was determined for all water and SPE-DOM samples as well as the yield of amino sugars for selected samples. Linear correlations were observed between the magnitudes of 43 % of the FT-ICR mass peaks and the extract Δ14C …

Tp53 Codon 72 Polymorphism Affects Accumulation Of Mtdna Damage In Human Cells, Serena Altilia, Aurelia Santoro, Davide Malagoli, Catia Lanzarini, Josué Adolfo Ballesteros Álvarez, Gianluca Galazzo, Donald Carl Porter, Paolina Crocco, Giuseppina Rose, Giuseppe Passarino, Igor Roninson, Claudio Franceschi, Stefano Salvioli

Faculty Publications

Human TP53 gene is characterised by a polymorphism at codon 72 leading to an Arginine-to-Proline (R/P) substitution. The two resulting p53 isoforms have a different subcellular localisation after stress (more nuclear or more mitochondrial for the P or R isoform, respectively). p53P72 variant is more efficient than p53R72 in inducing the expression of genes involved in nuclear DNA repair. Since p53 is involved also in mitochondrial DNA (mtDNA) maintenance, we wondered whether these p53 isoforms are associated with different accumulation of mtDNA damage. We observed that cells bearing p53R72 accumulate lower amount of mtDNA damage upon rotenone stress with respect …

Reproducing Value: How Tax Law Differentially Values Fertility, Sexuality & Marriage, Tessa R. Davis

Faculty Publications

Section 213 of the Internal Revenue Code permits a deduction for an individual’s fertility expenses, but it does not do so evenhandedly. This paper focuses on the current discriminatory effects of §213 doctrine as it is applied to the deductibility of fertility treatments for single persons and/or homosexual couples, as compared to heterosexual, married couples. Traditional economic analysis of the Code fails to explain such discrimination, thus a new approach is required. Utilizing tools from anthropological theory, this paper recognizes and analyzes our tax code (and specifically §213) as a cultural artifact and therein challenges the presumed objectivity of our …

Middle-Income Peers As Educational Resources And The Constitutional Right To Equal Access, Derek W. Black

Faculty Publications

Concentrated poverty in public schools continues to be a leading determinate of the educational opportunities that minority students receive. Since the effective end of mandatory desegregation, advocates have lacked legal tools to address it. As an alternative, some advocates and scholars have attempted to incorporate the concerns of concentrated poverty and racial segregation into educational litigation under state constitutions, but these efforts have been slow to take hold. Thus, all that has remained for students in poor and minority schools is the hope that school finance litigation could direct sufficient resources to mitigate their plight. This Article offers another solution. …

Corporate Social Responsibility After Disaster, Susan S. Kuo, Benjamin Means

Faculty Publications

In recent years, corporations have devoted substantial resources to disaster relief worldwide. For instance, Wal-Mart garnered favorable attention for its contributions in New Orleans and the Gulf Coast after Hurricane Katrina. According to company press releases, Wal-Mart recently gave hundreds of thousands of dollars for disaster relief in Brazil following a flood, and it has pledged millions in support of Japan in the wake of the tsunami.

Large corporations have not only the economic resources, but also the logistical capacity and operational expertise to make a difference in the first terrible days after a disaster. However, commentators disagree about how …

Decoupling Marriage & Procreation: A Feminist Argument For Same-Sex Marriage, Hannah Haksgaard

Faculty Publications

The quickly arriving reality of same-sex marriage is a move forward for all couples who marry, including opposite-sex and transgender couples. This piece examines the way that same-sex marriage will improve these other marriages. In particular, I argue that the "essential aspects" of marriage remain sex and reproduction - domains associated with the wife in a traditional marriage in a way that serves to denigrate women. Although the long-existing link between procreation and marriage has begun to fade, it will not be until same-sex marriage is fully legalized that opposite-sex marriage can fundamentally change. Therefore, both feminists and advocates of …

Anticancer Activity And Biophysical Reactivity Of Copper Complexes Of 2-(Benzo[D][1,3]Dioxol-5-Ylmethylene)-N-Alkylhydrazinecarbothioamides, Floyd A. Beckford, Jeffrey Thessing, Alyssa Stott, Alvin A. Holder, Oleg G. Poluektov, Liya Li, Navindra P. Seeram

Faculty Publications

A series of copper complexes were synthesized from benzo[d][1,3]dioxole-5-carbaldehyde (piperonal) thiosemicarbazones (RHpTSC where R = H, CH3, C2H5 or C6H5 (Ph)). The complexes show interesting variations in geometry depending on the thiosemicarbazone; a dinuclear complex [Cu(HpTSC)Cl]2, a mononuclear complex [Cu(RHpTSC)2Cl2] (R = CH3 or C2H5) and another mononuclear complex [Cu(PhHpTSC)(PhpTSC)Cl] was generated. The complexes bind in a moderately strong fashion to DNA with binding constants on the order of 10⁴ M^{− 1}. They are also strong binders of human serum albumin with binding constants near 10⁴ M^{− 1}. The complexes show good in vitro …

Report And Reflections From Year One Of The Nsf Gk- 12 Program At Usm, Sherry S. Herron, J. Hanna, K. Wingo

Faculty Publications

No abstract provided.

Challenges To Terry For The Twenty-First Century, Richard E. Myers Ii

Faculty Publications

No abstract provided.

Marine Snow Formation In The Aftermath Of The Deepwater Horizon Oil Spill In The Gulf Of Mexico, Uta Passow, K. Ziervogel, V. Asper, A. Diercks

Faculty Publications

The large marine snow formation event observed in oil-contaminated surface waters of the Gulf of Mexico (GoM) after the Deepwater Horizon accident possibly played a key role in the fate of the surface oil. We characterized the unusually large and mucus-rich marine snow that formed and conducted roller table experiments to investigate their formation mechanisms. Once marine snow lost its buoyancy, its sinking velocity, porosity and excess density were then similar to those of diatom or miscellaneous aggregates. The hydrated density of the component particles of the marine snow from the GoM was remarkably variable, suggesting a wide variety of …

The Weathering Of Oil After The Deepwater Horizon Oil Spill: Insights From The Chemical Composition Of The Oil From The Sea Surface, Salt Marshes And Sediments, Zhanfei Liu, Jiqing Liu, Qingzhi Zhu, Wei Wu

Faculty Publications

The oil released during the Deepwater Horizon (DWH) oil spill may have both short- and long-time impacts on the northern Gulf of Mexico ecosystems. An understanding of how the composition and concentration of the oil are altered by weathering, including chemical, physical and biological processes, is needed to evaluate the oil toxicity and impact on the ecosystem in the northern Gulf of Mexico. This study examined petroleum hydrocarbons in oil mousse collected from the sea surface and salt marshes, and in oil deposited in sediments adjacent to the wellhead after the DWH oil spill. Oil mousses were collected …

Discrimination Of Juvenile Red Snapper Otolith Chemical Signatures From Gulf Of Mexico Nursery Regions, Michelle Zapp Sluis, Beverly K. Barnett, William F. Patterson Iii, Alan M. Shiller

Faculty Publications

Age-0 red snapper Lutjanus campechanus from the 2005-2007 year-classes were sampled in six regions across the Gulf of Mexico (Gulf) to develop nursery signatures from otolith element : Ca ratios (Ba:Ca, Mg:Ca, Mn:Ca, Sr:Ca, and Li:Ca) and stable isotope delta values (delta C-13 and delta O-18). Element : Ca ratios were analyzed with sector field inductively coupled plasma mass spectrometry on dissolved right sagittae; isotope ratio mass spectrometry was employed to analyze pulverized left otoliths for delta C-13 and delta O-18. Otolith chemical signatures were significantly different among regions in each year. Year-class-specific quadratic discriminant function analysis (QDFA) distinguished nursery …

Minimalist Ensemble Algorithms For Genome-Wide Protein Localization Prediction, J.-R. Lin, A. M. Mondal, R. Liu, Jianjun Hu

Faculty Publications

Background

Computational prediction of protein subcellular localization can greatly help to elucidate its functions. Despite the existence of dozens of protein localization prediction algorithms, the prediction accuracy and coverage are still low. Several ensemble algorithms have been proposed to improve the prediction performance, which usually include as many as 10 or more individual localization algorithms. However, their performance is still limited by the running complexity and redundancy among individual prediction algorithms.

Results

This paper proposed a novel method for rational design of minimalist ensemble algorithms for practical genome-wide protein subcellular localization prediction. The algorithm is based on combining a feature …

The Friendly Yeti, Daniel S. Capper

Faculty Publications

Most images of yetis in Western popular culture and scholarly literature portray them as secular, predatory monsters. These representations overlook important religious dimensions of yetis that are hidden in the current literature, so I take a new look at yetis in Tibetan religions in order to clarify our understanding of these legendary creatures. Following a phenomenological approach that sets aside the issue of the ontological existence of yetis, I examine texts, art, ritual, and folklore in order to propose four yeti personal ideal types: the Buddhist practitioner, the human religious ally, the friendly yeti, and the mountain deity yeti. These …