Digital Commons Network^™

Hijacking Membrane Transporters For Arsenic Phytoextraction, Melissa S. Leblanc, Elizabeth C. Mckinney, Richard B. Meagher, Aaron P. Smith

Faculty Publications

Arsenic is a toxic metalloid and recognized carcinogen. Arsenate and arsenite are the most common arsenic species available for uptake by plants. As an inorganic phosphate (Pi) analog, arsenate is acquired by plant roots through endogenous Pi transport systems. Inside the cell, arsenate is reduced to the thiol-reactive form arsenite. Glutathione (GSH)-conjugates of arsenite may be extruded from the cell or sequestered in vacuoles by members of the ATP-binding cassette (ABC) family of transporters. In the present study we sought to enhance both plant arsenic uptake through Pi transporter overexpression, and plant arsenic tolerance through ABC transporter overexpression. We demonstrate …

Pan-Arctic Distributions Of Continental Runoff In The Arctic Ocean, Cédric G. Fichot, Karl Kaiser, Stanford B. Hooker, Rainer M.W. Amon, Marcel Babin, Simon Bélanger, Sally A. Walker, Ronald Benner

Faculty Publications

Continental runoff is a major source of freshwater, nutrients and terrigenous material to the Arctic Ocean. As such, it influences water column stratification, light attenuation, surface heating, gas exchange, biological productivity and carbon sequestration. Increasing river discharge and thawing permafrost suggest that the impacts of continental runoff on these processes are changing. Here, a new optical proxy was developed and implemented with remote sensing to determine the first pan-Arctic distribution of terrigenous dissolved organic matter (tDOM) and continental runoff in the surface Arctic Ocean. Retrospective analyses revealed connections between the routing of North American runoff and the recent freshening of …

Profluorogenic Reductase Substrate For Rapid, Selective, And Sensitive Visualization And Detection Of Human Cancer Cells That Overexpress Nqo1, William C. Silvers, Bijeta Prasai, David H. Burk, Matthew L. Brown, Robin L. Mccarley

Faculty Publications

Achieving the vision of identifying and quantifying cancer-related events and targets for future personalized oncology is predicated on the existence of synthetically accessible and economically viable probe molecules fully able to report the presence of these events and targets in a rapid and highly selective and sensitive fashion. Delineated here are the design and evaluation of a newly synthesized turn-on probe whose intense fluorescent reporter signature is revealed only through probe activation by a specific intracellular enzyme present in tumor cells of multiple origins. Quenching of molecular probe fluorescence is achieved through unique photoinduced electron transfer between the naphthalimide dye …

Profluorogenic Reductase Substrate For Rapid, Selective, And Sensitive Visualization And Detection Of Human Cancer Cells That Overexpress Nqo1, William C. Silvers, Bijeta Prasai, David H. Burk, Matthew L. Brown, Robin L. Mccarley

Faculty Publications

Achieving the vision of identifying and quantifying cancer-related events and targets for future personalized oncology is predicated on the existence of synthetically accessible and economically viable probe molecules fully able to report the presence of these events and targets in a rapid and highly selective and sensitive fashion. Delineated here are the design and evaluation of a newly synthesized turn-on probe whose intense fluorescent reporter signature is revealed only through probe activation by a specific intracellular enzyme present in tumor cells of multiple origins. Quenching of molecular probe fluorescence is achieved through unique photoinduced electron transfer between the naphthalimide dye …

Objectively Measured Sedentary Behavior In Preschool Children: Comparison Between Montessori And Traditional Preschools, Wonwoo Byun, Steven N. Blair, Russell R. Pate

Faculty Publications

Background: This study aimed to compare the levels of objectively-measured sedentary behavior in children attending Montessori preschools with those attending traditional preschools.

Methods: The participants in this study were preschool children aged 4 years old who were enrolled in Montessori and traditional preschools. The preschool children wore ActiGraph accelerometers. Accelerometers were initialized using 15-second intervals and sedentary behavior was defined as/15-second. The accelerometry data were summarized into the average minutes per hour spent in sedentary behavior during the in-school, the afterschool, and the total-day period. Mixed linear regression models were used to determine differences in the average time spent in …

Transcription Factor Yy1 Is Essential For Regulation Of The Th2 Cytokine Locus And For Th2 Cell Differentiation, Soo Seok Hwang, Young Uk Kim, Sumin Lee, Sung Woong Jang, Min Kyung Kim, Byung Hee Koh, Wonyong Lee, Joomyeong Kim, Abdallah Souabni, Meinrad Busslinger, Gap Ryol Lee

Faculty Publications

The Th2 locus control region (LCR) has been shown to be important in efficient and coordinated cytokine gene regulation during Th2 cell differentiation. However, the molecular mechanism for this is poorly understood. To study the molecular mechanism of the Th2 LCR, we searched for proteins binding to it. We discovered that transcription factor YY1 bound to the LCR and the entire Th2 cytokine locus in a Th2-specific manner. Retroviral overexpression of YY1 induced Th2 cytokine expression. CD4-specific knockdown of YY1 in mice caused marked reduction in Th2 cytokine expression, repressed chromatin remodeling, decreased intrachromosomal interactions, and resistance in an animal …

Narratives Of Longevity: Why Adventist Principals Stay Beyond 10 Years In One School., Janet Ledesma

Faculty Publications

Across public and private school systems, there is a shortage of principals in the United States. Many studies have identified reasons why school principals leave their jobs. However, few studies have been conducted to understand why principals stay. The purpose of this article is to describe the experiences of 14 principals who have remained in leadership in the Seventh- day Adventist system of education in North America longer than 10 years in a single school building. Though the lens of resiliency theory and spiritual leadership theory, meaning was captured i the lived experiences of these Christian educational leaders.

Loving The Questions: Finding Food For The Future Of Theological Education In The Lexington Seminar, Mary E. Hess

Faculty Publications

Although it ran for more than ten years and involved more than 200 faculty from forty-four ATS member schools, the findings of the Lexington Seminar have not been engaged as robustly as they could be in facing current challenges. This essay collates the experiences of the Lexington Seminar with recent educational literature to suggest a range of options in faculty development for meeting the adaptive challenges facing schools, particularly in terms of shifting dynamics of authority, authenticity, and agency.

Growing Up Trans: Socialization And The Gender Binary [Abstract], Michelle Dietert, Dianne Dentice

Faculty Publications

No abstract provided.

Evaluation Of Electrostatic Particle Ionization And Biocurtain™ Technologies To Reduce Air Pollutants From Broiler Houses, Sheryll B. Jerez, W Faulkner, K. D. Casey, M S. Borhan, R. A. Smith

Faculty Publications

The continuing growth of poultry production, along with the increasing urbanization of rural areas, is leading to more odor-related complaints from neighboring communities and more scrutiny from policy makers. It is, therefore, in the best interest of poultry producers to look at control methods for abating odors. Previous studies have shown that substantial amounts of volatile and odorous compounds are adsorbed and transported by dust particles. Thus, by reducing the amount of dust emitted from poultry facilities such as broiler houses, odor may be reduced as well. The objective of this study was to evaluate the effectiveness of two commercially …

Accounting For Agricultural Products: Us Versus Ifrs Gaap, Treba Marsh, Mary Fischer

Faculty Publications

Currently there is a mix of accounting guidance for agriculture producers in the US that is both GAAP including Accounting Statement Codification 905 and non-GAAP financial guidelines. Should the US adopt International Financial Reporting Standards (IFRS), this guidance would be replaced with International Accounting Standard (IAS) 41 – Agriculture.

This study identifies systematic differences between the US and International accounting and reporting for agricultural assets and products. The study also finds that international and US agricultural accounting recognition and reporting guidance result in dissimilar reporting due to guidance interpretation. Valuation variances and definition differences including the requirement to change the …

Female-Only Classes In A Rural Context: Self-Concept, Achievement, And Discourse, Hope E. Wilson, Gloria J. Gresham Dr., Michelle Williams, Claudia Whitley, James G. Partin

Faculty Publications

Two middle schools in rural east Texas implemented an optional, single-sex program. Although previous studies have documented the effects of single-sex instruction, and recent educational innovations have focused on its benefits, little research has investigated its effects in rural contexts. This study found that for rural populations, patterns of discourse differ between female-only and coeducational classes, with all-female classes participating in higher levels of thinking and engaging in more discourse overall. However, girls in single-sex classes had significantly lower levels of general self-concept than girls in coeducational classes. There were no statistically significant differences in the achievement test scores between …

Hunger And Reduced Self-Control In The Laboratory And Across The World: Reducing Hunger As A Self-Control Panacea, Matthew T. Gailliot

Faculty Publications

Ten studies link hunger to reduced self-control. Higher levels of hunger-as assessed by self-report, time since last eating, or physiology-predicted reduced self-control, as indicated by increased racial prejudice, (hypothetical) sexual infidelity, passivity, accessibility of death thoughts and perceptions of task difficulty, as well as impaired Stroop performance and decreased self-monitoring. Increased rates of hunger across 200 countries predicted increased war killings, suggestive of reduced aggressive restraint. In a final experiment, self-reported hunger mediated the effect of hungry (v fed) participants performing worse on the Stroop task, suggesting a causal relationship of hunger reducing self-control.

Productive Resources In Students’ Ideas About Energy: An Alternative Analysis Of Watts’ Original Interview Transcripts, Benedikt W. Harrer, Virginia J. Flood, Michael C. Wittmann

Faculty Publications

For over 30 years, researchers have investigated students’ ideas about energy with the intent of reforming instructional practice. In this pursuit, Watts contributed an influential study with his 1983 paper “Some alternative views of energy” [Phys. Educ. 18, 213 (1983)]. Watts’ “alternative frameworks” continue to be used for categorizing students’ non-normative ideas about energy. Using a resources framework, we propose an alternate analysis of student responses from Watts’ interviews. In our analysis, we show how students’ activated resources about energy are disciplinarily productive. We suggest that fostering seeds of scientific understandings in students’ ideas about energy may play an important …

Contact Hypersensitivity To Oxazolone Provokes Vulvar Mechanical Hyperalgesia In Mice, Tijana Martinov, Rose Glenn-Finer, Sarah Burley, Elena Tonc, Evelyn Balsells, Alyssa Ashbaugh, Linnea Swanson, Randy S. Daughters, Devavani Chatterjea

Faculty Publications

The interplay among pain, allergy and dysregulated inflammation promises to yield significant conceptual advances in immunology and chronic pain. Hapten-mediated contact hypersensitivity reactions are used to model skin allergies in rodents but have not been utilized to study associated changes in pain perception in the affected skin. Here we characterized changes in mechanical hyperalgesia in oxazolone-sensitized female mice challenged with single and repeated labiar skin exposure to oxazolone. Female mice were sensitized with topical oxazolone on their flanks and challenged 1-3 times on the labia. We then measured mechanical sensitivity of the vulvar region with an electronic pressure meter and …

Ethnobotany, Phytochemistry, And Pharmacological Activities Of Taxodium Rich, Zushang Su

Faculty Publications

Taxodium Rich. is a genus of the family Cupressaceae. The trees are especially prized for their rot and termite resistant wood. Taxodium leaves and cones, which are particularly rich in essential oils were used as folk medicine to treat skin, gastro-intestinal, respiratory, inflammation, and infections. Preliminary bio-activity assays reported antimicrobial, antitumor, antitermitic, antispasmodic and bronchodilator activities of the extracts or isolates from Taxodium but further pharmacological investigations of bio-active compounds are not available. To data, at least 130 compounds have been identified from extant or fossil Taxodium. Fifty-seven compounds have been isolated primarily from the cones, leaves,and branches of extant …

Occupational Engagement And Health In Older South Asian Immigrants, Sheama R. Krishnagiri, Erin Fuller, Lesley Ruda, Sadhna Diwan

Faculty Publications

The purpose of this study was to investigate the elements that shape how healthy active South Asian elder immigrants engage in daily activities to maintain their health. A descriptive grounded theory approach was employed using semi-structured interviews and a daily activity chart. Convenience sampling was used to recruit 12 South Asian elders, 7 males and 5 females, who immigrated after retirement to the South San Francisco Bay area. Open, axial, and selective coding of the transcribed data led to two broad themes, control and interdependence. These themes may be viewed as dynamic and interconnected forces that shape and determine the …

Effects Of Storage Conditions On The Morphology And Titer Of Lentiviral Vectors, H. Rahman, Josephine Taylor, Beatrice Clack, R. S. Stewart, Sarah C. Canterberry

Faculty Publications

Lentiviral vectors are commonly used in laboratory experiments to stably integrate transgenes into host genomes. It has long been observed that storage of virus stocks leads to a decrease in viral titer, but the mechanisms driving this decrease have yet to be identified. To that end, lentiviral vector stocks were generated and stored as follows: room temperature for less than one hour, -80ºC for 24 hours, 4ºC for three days and 4ºC for 7 days. These stocks were subsequently evaluated with regard to their transducing ability and their morphology, specifically particle diameter. The vector that was stored at room temperature …

Differential Habitat Use By Common Watersnakes (Nerodia Sipedon), Lorin A. Neuman-Lee, Andrew M. Durso, Nicholas M. Kiriazis, Melanie J. Olds, Stephen J. Mullin

Faculty Publications

Understanding intraspecific variation in habitat use is important for the management of any species. In many studies of reptiles, habitat use by juveniles is poorly understood when compared to their adult conspecifics because of capture biases and logistical constraints. We compared habitat use between sexes and age classes of Common Watersnakes (Nerodia sipedon) at a reservoir in central Illinois. Juvenile N. sipedon occurred more frequently in habitat with high canopy cover, whereas adult N. sipedon, especially reproductive females, were found exclusively in habitat with no canopy cover. Adult males used both locations equally. We emphasize the need to investigate ontogenetic …

Differential Jumping Performance In Newly Metamorphosed Blanchard's Cricket Frogs, Acris Blanchardi (Anura: Hylidae), From Fish- And Invertebrate-Dominated Ponds, Taylor L. Hall, Daniel Saenz, Matthew Kwiatkowski

Faculty Publications

Organisms that adopt phenotypically plastic anti-predator strategies during larval stages may incur fitness costs later in development. These costs are typically difficult to define in many species. The difficulty of identifying ecological trade-offs may result from experimental comparisons that do not adequately mirror naturally occurring predator-prey relatioships. To examine this, we captured 61 newly metamorphosed Acris blanchardi from ponds dominated either by fish or invertebrate predators. These predators are known to induce alternate phenotypic anti-predator responses at the larval stage. We use jumping performance as a measure of post-metamorphic fitness, and compared the morphological traits and jumping ability of frogs …

Synergistic Effects Of The Invasive Chinese Tallow (Triadica Sebifera) And Climate Change On Aquatic Amphibian Survival, Daniel Saenz, Erin M. Fucik, Matthew Kwiatkowski

Faculty Publications

Changes in climate and the introduction of invasive species are two major stressors to amphibians, although little is known about the interaction between these two factors with regard to impacts on amphibians. We focused our study on an invasive tree species, the Chinese tallow (Triadica sebifera), that annually sheds its leaves and produces leaf litter that is known to negatively impact aquatic amphibian survival. The purpose of our research was to determine whether the timing of leaf fall from Chinese tallow and the timing of amphibian breeding (determined by weather) influence survival of amphibian larvae. We simulated a …

A Dft Study Of Vibrational Spectra And Mutagenicity Predictions Of Mononitrated Fluoranthenes [Abstract], Kefa Karimu Onchoke, Jorge Ojeda

Faculty Publications

No abstract provided.

Assessment Of Genetic Diversity In Peruvian Amaranth (Amaranthus Caudatus And A. Hybridus) Germplasm Using Single Nucleotide Polymorphism Markers [Abstract], Felix Jimenez, Peter J. Maughan, Aquilino Alvarez, Kiel D. Kielinski, Scott M. Smith, Donald B. Pratt, David B. Elzinga, Eric N. Jellen

Faculty Publications

No abstract provided.

Ammonium Sulfate Precipitation Combined With Liquid Chromatography Is Sufficient For Purification Of Bovine Serum Albumin That Is Suitable For Most Routine Laboratory Applications, Odutayo O. Odunuge, Alina Shazhko

Faculty Publications

The use of bovine serum albumin (BSA) in routine biochemical assays such as restriction enzyme digestion and immunodetection is plagued largely by two common contaminants, DNase and immunoglobulins G (IgGs). Acetylation of BSA to inactivate DNase limits its use as a protein standard due to interference with color development in assays such as Lowry’s. In spite of the availability of inexpensive BSA, its purification involves several cumbersome and time-consuming steps. In this work, we employed a modified strategy of ammonium sulfate precipitation coupled with liquid chromatography to purify BSA that is free of DNase and IgGs. Purified BSA tested negative …

Students Talk About Energy In Project- Based Inquiry Science, Benedikt W. Harrer, Virginia J. Flood, Michael C. Wittmann

Faculty Publications

We examine the types of emergent language eighth grade students in rural Maine middle schools use when they discuss energy in their first experiences with Project-Based Inquiry Science: Energy, a research-based curriculum that uses a specific language for talking about energy. By comparative analysis of the language used by the curriculum materials to students’ language, we find that students’ talk is at times more aligned with a Stores and Transfer model of energy than the Forms model supported by the curriculum.

The Supreme Court Screws Up The Science: There Is No Abusive Head Trauma/Shaken Baby Syndrome “Scientific” Controversy, Joelle A. Moreno, Brian Holmgren

Faculty Publications

No abstract provided.

Influence Of Translocations On Eastern Wild Turkey Population Genetics In Texas, Christopher E. Comer, Warren C. Conway, Gary Calkins, Sabrina Seidel, Randy Deyoung, Jason Hardin

Faculty Publications

Between 1979 and 2006, over 7,000 eastern wild turkeys (Meleagris gallopavo silvestris) from 16 states were translocated to east Texas in an attempt to restore a stable, huntable population. Although current populations are stable in some areas and a spring male-only hunting season was opened in 1995, turkey density in the region remains low and large areas of apparently suitable habitat are not occupied. The longterm effects of the extensive translocations and current levels of connectivity among various populations are unknown. We used microsatellite DNA analysis to assess the influence of translocations on current genetic structure and gene flow in …

Using Gis-Based, Regional Extent Habitat Suitability Modeling To Identify Conservation Priority Areas: A Case Study Of The Louisiana Black Bear In East Texas, Christopher E. Comer, I-Kuai Hung, Gary Calkins, Dan J. Kaminski, Nathan Garner

Faculty Publications

State and federal recovery plans mandate that priority areas for future population expansion be identified within the historical range of the Louisiana black bear (Ursus americanus luteolus). Despite the presence of potentially suitable habitat in east Texas and expanding populations in adjacent states, quantitative estimates of regional habitat suitability do not exist. We developed a regional extent habitat suitability index (HSI) model in a geographic information system (GIS) to evaluate year-round habitat requirements for black bears in the 43,530-km2 south black bear recovery zone in southeastern Texas. We measured hard and soft mast production, understory vegetation density, and tree den …

Seasonal Variation In Offspring Sex Ratio In The Snowy Plover, Sarah T. Saalfeld, Warren C. Conway, David A. Haukos, William P. Johnson

Faculty Publications

The Snowy Plover (Charadrius nivosus) is unique in being a determinate layer of an odd modal clutch size and in having a variable mating system in which female brood desertion occurs regularly. These traits make determining Snowy Plover offspring sex ratios important not only for long-term population stability, as the species is of conservation concern, but also for application to sex allocation theory. In this study, we determined Snowy Plover offspring sex ratios, examined differential costs of producing male and female offspring, and evaluated sex ratio variation in relation to maternal condition, habitat condition, and time during the …

Psychologists And Medications In The Era Of Interprofessional Care: Collaboration Is Less Problematic And Costly Than Prescribing, William N. Robiner, Tim R. Tumlin, Tanya Tompkins

Faculty Publications

Increasing emphasis on interprofessionalism and teamwork in healthcare renders psychologists’ collaborations critical and invites reexamination of psychologists’ roles related to medications. The Collaboration Level outlined by the APA’s Ad Hoc Task Force is more achievable and in synch with health reform than prescription privileges (RxP). RxP remains controversial due to training and safety concerns, lacking support from health professionals, psychologists, and consumers. Differences in educational preparation of psychologists relative to prescribing professionals are discussed. Enactment of only three of 170 RxP initiatives reveals RxP to be a costly, ineffectual agenda. Alternatives (e.g., integrated care, collaboration, telehealth) increase access without risks …