Digital Commons Network^™

Increasing Label-Free Stem Cell Sorting Capacity To Reach Transplantation-Scale Throughput, Melinda Simon, Ying Li, Janahan Arulmoli, Lisa Mcdonnell, Adnan Akil, Jamison Nourse, Abraham Lee, Lisa Flanagan

Faculty Publications

Dielectrophoresis (DEP) has proven an invaluable tool for the enrichment of populations of stem and progenitor cells owing to its ability to sort cells in a label-free manner and its biological safety. However, DEP separation devices have suffered from a low throughput preventing researchers from undertaking studies requiring large numbers of cells, such as needed for cell transplantation. We developed a microfluidic device designed for the enrichment of stem and progenitor cell populations that sorts cells at a rate of 150,000 cells/h, corresponding to an improvement in the throughput achieved with our previous device designs by over an order of …

Exposure To Combustion Generated Environmentally Persistent Free Radicals Enhances Severity Of Influenza Virus Infection, Greg I. Lee, Jordy Saravia, Dahui You, Bishwas Shrestha, Sridhar Jaligama, Valerie Y. Hebert, Tammy R. Dugas, Stephania A. Cormier

Faculty Publications

© 2014 Lee et al. Background: Exposures to elevated levels of particulate matter (PM) enhance severity of influenza virus infection in infants. The biological mechanism responsible for this phenomenon is unknown. The recent identification of environmentally persistent free radicals (EPFRs) associated with PM from a variety of combustion sources suggests its role in the enhancement of influenza disease severity. Methods: Neonatal mice (< seven days of age) were exposed to DCB230 (combustion derived PM with a chemisorbed EPFR), DCB50 (non-EPFR PM sample), or air for 30 minutes/day for seven consecutive days. Four days post-exposure, neonates were infected with influenza intranasally at 1.25 TCID50/neonate. Neonates were assessed for morbidity (% weight gain, peak pulmonary viral load, and viral clearance) and percent survival. Lungs were isolated and assessed for oxidative stress (8-isoprostanes and glutathione levels), adaptive immune response to influenza, and regulatory T cells (Tregs). The role of the EPFR was also assessed by use of transgenic mice expressing human superoxide dismutase 2. Results: Neonates exposed to EPFRs had significantly enhanced morbidity and decreased survival following influenza infection. Increased oxidative stress was also observed in EPFR exposed neonates. This correlated with increased pulmonary Tregs and dampened protective T cell responses to influenza infection. Reduction of EPFR-induced oxidative stress attenuated these effects. Conclusions: Neonatal exposure to EPFR containing PM resulted in pulmonary oxidative stress and enhanced influenza disease severity. EPFR-induced oxidative stress resulted in increased presence of Tregs in the lungs and subsequent suppression of adaptive immune response to influenza.

A Single Intramuscular Vaccination Of Mice With The Hsv-1 Vc2 Virus With Mutations In The Glycoprotein K And The Membrane Protein Ul20 Confers Full Protection Against Lethal Intravaginal Challenge With Virulent Hsv-1 And Hsv-2 Strains, Brent A. Stanfield, Jacque Stahl, Vladimir N. Chouljenko, Ramesh Subramanian, Anu Susan Charles, Ahmad A. Saied, Jason D. Walker, Konstantin G. Kousoulas

Faculty Publications

© 2014 Stanfield et al. Herpes Simplex Virus type-1 (HSV-1) and type-2 (HSV-2) establish life-long infections and cause significant orofacial and genital infections in humans. HSV-1 is the leading cause of infectious blindness in the western world. Currently, there are no available vaccines to protect against herpes simplex infections. Recently, we showed that a single intramuscular immunization with an HSV-1(F) mutant virus lacking expression of the viral glycoprotein K (gK), which prevents the virus from entering into distal axons of ganglionic neurons, conferred significant protection against either virulent HSV-1(McKrae) or HSV-2(G) intravaginal challenge in mice. Specifically, 90% of the mice …

Data Access For The 1,000 Plants (1kp) Project, Naim Matasci, Ling Hong Hung, Zhixiang Yan, Eric J. Carpenter, Norman J. Wickett, Siavash Mirarab, Nam Nguyen, Tandy Warnow, Saravanaraj Ayyampalayam, Michael Barker, J. G. Burleigh, Matthew A. Gitzendanner, Eric Wafula, Joshua P. Der, Claude W. Depamphilis, Béatrice Roure, Hervé Philippe, Brad R. Ruhfel, Nicholas W. Miles, Sean W. Graham, Sarah Mathews, Barbara Surek, Michael Melkonian, Douglas E. Soltis, Pamela S. Soltis, Carl Rothfels, Lisa Pokorny, Jonathan A. Shaw, Lisa Degironimo, Dennis W. Stevenson, Juan C. Villarreal, Tao Chen, Toni M. Kutchan

Faculty Publications

© 2014 Matasci et al.; licensee BioMed Central Ltd. The 1,000 plants (1KP) project is an international multi-disciplinary consortium that has generated transcriptome data from over 1,000 plant species, with exemplars for all of the major lineages across the Viridiplantae (green plants) clade. Here, we describe how to access the data used in a phylogenomics analysis of the first 85 species, and how to visualize our gene and species trees. Users can develop computational pipelines to analyse these data, in conjunction with data of their own that they can upload. Computationally estimated protein-protein interactions and biochemical pathways can be visualized …

Economic Evaluation Of An Area-Wide Integrated Pest Management Program To Control The Asian Tiger Mosquito In New Jersey, Donald S. Shepard, Yara A. Halasa, Dina M. Fonseca, Ary Farajollahi, Sean P. Healy, Randy Gaugler, Kristen Bartlett-Healy, Daniel A. Strickman, Gary G. Clark

Faculty Publications

Aedes albopictus is the most invasive mosquito in the world, an important disease vector, and a biting nuisance that limits outdoor activities. Area-wide integrated pest management (AW-IPM) is the recommended control strategy. We conducted an economic evaluation of the AW-IPM project in Mercer and Monmouth Counties, New Jersey with a controlled design (AW-IPM vs. control) from 2009 through 2011. The study analyzed financial documents and staff time for AW-IPM and surveyed an average of 415 randomly chosen households in AW-IPM and control areas each fall from 2008 through 2011. Hours lost from yard and porch activities were calculated as differences …

Seqassist: A Novel Toolkit For Preliminary Analysis Of Next-Generation Sequencing Data, Yan Peng, Andrew S. Maxwell, Natalie D. Barker, Jennifer G. Laird, Alan J. Kennedy, Nan Wang, Chaoyang Zhang, Ping Gong

Faculty Publications

Background: While next-generation sequencing (NGS) technologies are rapidly advancing, an area that lags behind is the development of efficient and user-friendly tools for preliminary analysis of massive NGS data. As an effort to fill this gap to keep up with the fast pace of technological advancement and to accelerate data-to-results turnaround, we developed a novel software package named SeqAssist ("Sequencing Assistant" or SA).

Results: SeqAssist takes NGS-generated FASTQ files as the input, employs the BWA-MEM aligner for sequence alignment, and aims to provide a quick overview and basic statistics of NGS data. It consists of three separate workflows: …

Proceedings Of The 2014 Midsouth Computational Biology And Bioinformatics Society (Mcbios) Conference, Jonathan D. Wren, Mikhail G. Dozmorov, Dennis Burian, Andy Perkins, Chaoyang Zhang, Peter Hoyt, Rakesh Kaundal

Faculty Publications

No abstract provided.

Psychological Fears Among Low-Paid Female Sex Workers In Southwest China And Their Implications For Hiv Prevention, Shan Qiao Ph.D., Xiaoming Li Ph.D., Chen Zhang, Yuejiao Zhou, Zhiyong Shen, Zhenzhu Tang, Bonita Stanton

Faculty Publications

Commercial sex plays a critical role in rapidly increasing heterosexual transmission of HIV in China. Low-paid female sex workers (FSWs) are especially vulnerable to HIV/AIDS. Because of the illegality and stigma associated with sex work, FSWs may constantly live with fears in their daily life. Based on cross-sectional study of 794 low-paid FSWs in China we described their psychological fears related to commercial sex and examined the associations between fears and HIV-related behaviors. Fear of HIV infection was significantly associated with consistent use of condoms with clients. However, fear of breaching sex worker identity significantly prevented the FSWs from consistently …

Amplification Of Angular Rotations Using Weak Measurements, Omar S. Magaña-Loaiza, Mohammad Mirhosseini, Brandon Rodenburg, Robert W. Boyd

Faculty Publications

We experimentally demonstrate the first weak-value amplification of the azimuthal degree of freedom. We estimate rotations using shifts in angular position and orbital angular momentum, via spin-orbit coupling and an applied geometric phase.

Residency Time As An Indicator Of Reproductive Restraint In Male Burying Beetles, Ashlee N. Smith, Mark C. Belk, J. Curtis Creighton

Faculty Publications

The cost of reproduction theory posits that there are trade-offs between current and future reproduction because resources that are allocated to current offspring cannot be used for future reproductive opportunities. Two adaptive reproductive strategies have been hypothesized to offset the costs of reproduction and maximize lifetime fitness. The terminal investment hypothesis predicts that as individuals age they will allocate more resources to current reproduction as a response to decreasing residual reproductive value. The reproductive restraint hypotheses predicts that as individuals age they will allocate fewer resources to current reproduction to increase the chance of surviving for an additional reproductive opportunity. …

Health Literacy And Obesity Among Native Hawaiian And Pacific Islanders In The U.S., Jane Lassetter

Faculty Publications

Objectives: Our purpose was to describe relationships between demographic characteristics, body mass index (BMI), and health literacy among Native Hawaiians and other Pacific Islanders (NHPIs).

Design and Sample: In this cross-sectional survey, we interviewed 364 NHPI adults.

Measures: We used Newest Vital Sign (NVS), a health literacy tool; measured heights and weights; and demographic questions.

Results: According to participants’ NVS scores, 45.3% had at least a possibility of low health literacy. Lower NVS scores were associated with increased BMI (r = -0.12, p = 0.027) and increased age (r = -0.26, p < 0.001). Higher NVS scores were associated …

Assessment Of The Adoption Of Agroforestry Technologies By Limited-Resource Farmers In North Carolina, Paula E. Faulkner, Bismark Owooh, Joshua Idassi

Faculty Publications

Agroforestry is a natural resource management system that integrates trees, forages, and livestock. The study reported here was conducted to determine farmers' knowledge about and willingness to adopt agroforestry technologies in North Carolina. The study reported participants were primarily older, male farmers, suggesting the need to attract more females and younger individuals to adopt agroforestry technologies. The increasing number of diversified farm operators presents a new audience for Extension educators to offer programs to improve limited-resource farmers' livelihood. The study recommends Extension training programs and information centers for farmers who need skills and knowledge to manage agroforestry technologies.

Bullish On Forestry Careers, Steven H. Bullard, Thomas J. Straka

Faculty Publications

During the next decade, baby boomer-aged foresters, those born from 1946 to 1964, will be retiring in large numbers. This retirement trend is true for all sectors of forestry employment, from state and federal agencies to private firms and industries.

Forestry employers in both public and private sectors are clamoring for more graduates, as well as for more diverse graduates, from forestry degree programs. With looming retirements and industry growth, employers can project the number of positions opening, and they are aware that U.S. forestry schools are not producing sufficient graduates to fill the positions.

A Comparison Of Pragmatic Language In Boys With Autism And Fragile X Syndrome, Jessica Klusek, Gary E. Martin, Molly Losh

Faculty Publications

No abstract provided.

Social Regulation Of Reproduction In Male Cichlid Fishes, Karen P. Maruska

Faculty Publications

© 2014 Elsevier Inc. Social interactions and relative positions within a dominance hierarchy have helped shape the evolution of reproduction in many animals. Since reproduction is crucial in all animals, and rank typically regulates access to reproductive opportunities, understanding the mechanisms that regulate socially-induced reproductive processes is extremely important. How does position in a dominance hierarchy impact an individual's reproductive behavior, morphology, and physiology? Teleost fishes, and cichlids in particular, are ideally-suited models for studying how social status influences reproduction on multiple levels of biological organization. Here I review the current knowledge on the reproductive behavioral and physiological consequences of …

The Early History Of Chance In Evolution, Charles H. Pence

Faculty Publications

Work throughout the history and philosophy of biology frequently employs ‘chance’, ‘unpredictability’, ‘probability’, and many similar terms. One common way of understanding how these concepts were introduced in evolution focuses on two central issues: the first use of statistical methods in evolution (Galton), and the first use of the concept of “objective chance” in evolution (Wright). I argue that while this approach has merit, it fails to fully capture interesting philosophical reflections on the role of chance expounded by two of Galton's students, Karl Pearson and W.F.R. Weldon. Considering a question more familiar from contemporary philosophy of biology—the relationship between …

Contributions Of Non-Traditional Meat Animals To Global Food Security And Agricultural Economy, Surendranath P. Suman, Kenneth W. Mcmillin

Faculty Publications

No abstract provided.

Anonymization Of Dicom Electronic Medical Records For Radiation Therapy, Wayne Newhauser, Timothy Jones, Stuart Swerdloff, Warren Newhauser, Mark Cilia, Robert Carver, Andy Halloran, Rui Zhang

Faculty Publications

Electronic medical records (EMR) and treatment plans are used in research on patient outcomes and radiation effects. In many situations researchers must remove protected health information (PHI) from EMRs. The literature contains several studies describing the anonymization of generic Digital Imaging and Communication in Medicine (DICOM) files and DICOM image sets but no publications were found that discuss the anonymization of DICOM radiation therapy plans, a key component of an EMR in a cancer clinic. In addition to this we were unable to find a commercial software tool that met the minimum requirements for anonymization and preservation of data integrity …

Comparison Of Infrapatellar And Subcutaneous Adipose Tissue Stromal Vascular Fraction And Stromal/Stem Cells In Osteoarthritic Subjects, Pedro Pires De Carvalho, Katie M. Hamel, Robert Duarte, Andrew G.S. King, Masudul Haque, Marilyn A. Dietrich, Xiying Wu, Forum Shah, David Burk, Rui L. Reis, Jennifer Rood, Ping Zhang, Mandi Lopez, Jeffrey M. Gimble, Vinod Dasa

Faculty Publications

© 2012 John Wiley & Sons, Ltd. Since inflammatory mechanisms have been postulated to link obesity to osteoarthritis, the current study evaluated the ratio of immune cells to multipotent stromal cells within the infrapatellar fat pad (IPFP) and subcutaneous adipose tissue (SQ) of the knee; each depot has potential as a source of regenerative cells. The immunophenotypes of stromal vascular fraction (SVF) and adipose-derived stem cells (ASCs) of the IPFP and SQ were determined in tissues from osteoarthritic subjects (n=7) undergoing total knee replacement. Based on a subset of surface antigens, the immunophenotype of ASCs from SQ of OA subjects …

Influence Of Season And Method Of Topkill On Resprouting Characteristics And Biomass Of Quercus Nigra Saplings From A Southeastern U.S. Pine-Grassland Ecosystem, Tracy L. Hmielowski, Kevin M. Robertson, William J. Platt

Faculty Publications

© 2014, Springer Science+Business Media Dordrecht. The resprouting ability of woody plants in frequently burned ecosystems may be influenced by the season and method of topkill. We conducted an experiment to test for the effects of season and method of topkill on aboveground biomass, belowground biomass, and mortality of hardwoods found in a southeastern U.S. pine-grassland. We predicted that topkill occurring during the growing season and topkill by fire would have greater negative impacts on resprouting and root growth and result in greater mortality. We conducted a shadehouse experiment in north Florida in which we applied topkill treatments (burn, clip, …

Development Of Ultrasound Sensitive Eliposomes Containing Doxorubicin For Drug Delivery, Chung-Yin Lin, William G. Pitt, Kuo-Chen Wei

Faculty Publications

Aims: A novel nanocarrier was formulated by remote loading of doxorubicin (Dox) into a dipalmitoylphosphatidylcholine (DPPC) liposome that also contains various perfluorocarbon (PFC) droplets within its aqueous interior. It was shown that Dox can be loaded to a level of up to 67% into these large unilamellar vesicles composed of DPPC and cholesterol by employing a transmembrane pH gradient technique.

Methods: The different encapsulation efficiencies for these eLipoDox constructs of differing PFC composition are 45.5% (PFC5), 31.5% (PFC6) and 66.7% (PFC5/PFC6 mixture, PFCm). At 30 seconds of insonation, the eLipoDox formulation with PFCm droplets appeared to release more Dox than …

A Comparison Of The Caulobacter Na1000 And K31 Genomes Reveals Extensive Genome Rearrangements And Differences In Metabolic Potential, Kurt Ash, Theta Brown, Tynetta Watford, Latia E. Scott, Craig Stephens, Bert Ely

Faculty Publications

The genus Caulobacter is found in a variety of habitats and is known for its ability to thrive in low-nutrient conditions. K31 is a novel Caulobacter isolate that has the ability to tolerate copper and chlorophenols, and can grow at 4°C with a doubling time of 40 h. K31 contains a 5.5 Mb chromosome that codes for more than 5500 proteins and two large plasmids (234 and 178 kb) that code for 438 additional proteins. A comparison of the K31 and the Caulobacter crescentus NA1000 genomes revealed extensive rearrangements of gene order, suggesting that the genomes had been randomly scrambled. …

Review Of The Face Of The Earth, Ann E. Lundberg

Faculty Publications

Review of SueEllen Campbell, et al. The Face of the Earth: Natural Landscapes, Science, and Culture. Published review is at Western American Literature.

A Test Of The Mean Distance Method For Forest Regeneration Assessment, Daniel Unger, Jeremy P. Stovall, Brian P. Oswald, David Kulhavy, I-Kuai Hung

Faculty Publications

A new distance-based estimator for forest regeneration assessment, the mean distance method, was developed by combining ideas and techniques from the wandering quarter method, T-square sampling and the random pairs method. The performance of the mean distance method was compared to conventional 4.05 square meter plot sampling through simulation analysis on 405 square meter blocks of a field surveyed clumped distribution and a computer generated random distribution at different levels of density of 100, 50 and 25%. The mean distance method accurately estimated density on the random populations but the mean distance method estimates were more variable than those of …

Paternally Expressed Peg3 Controls Maternally Expressed Zim1 As A Trans Factor, An Ye, Hongzhi He, Joomyeong Kim

Faculty Publications

© 2014 Ye et al. The expression of two adjacent imprinted genes, Peg3 and Zim1, is inversely correlated: down-regulation of Peg3 coinciding with up-regulation of Zim1. The current study characterized this inverse correlation using a mutant allele targeting Peg3. According to the results, the mutation on the paternal allele of Peg3 resulted in a dramatic increase in the transcription levels of the maternal allele of Zim1, suggesting the involvement of unknown trans factors in this trans-allelic event. Subsequent ChIP experiments revealed that the protein encoded by Peg3 itself binds to the zinc finger exon of Zim1, which is modified with …

Investigating The Stability Of Eliposomes At Elevated Temperatures, Ghaleb A. Husseini, William G. Pitt, Marjan Javadi

Faculty Publications

eLiposomes encapsulate a perfluorocarbon nanoemulsion droplet inside a liposome. Ultrasound is then used as a trigger mechanism to vaporize the perfluorocarbon, break the liposome, and release the desired drug to the tumor tissue. The purpose of this research is to show that eLiposomes synthesized using perfluoropentane are stable above the normal boiling point of the perfluoropentane and at body temperature and thus has potential for use in vivo. Experiments involving the release of fluorescent calcein molecules were performed on eLiposomes to measure the release of calcein at various temperatures in the absence of ultrasound. Results showed that eLiposomes are stable …

Investigating The Stability Of Eliposomes At Elevated Temperatures, Ghaleb A. Husseini, William G. Pitt, Marjan Javadi

Faculty Publications

eLiposomes encapsulate a perfluorocarbon nanoemulsion droplet inside a liposome. Ultrasound is then used as a trigger mechanism to vaporize the perfluorocarbon, break the liposome, and release the desired drug to the tumor tissue. The purpose of this research is to show that eLiposomes synthesized using perfluoropentane are stable above the normal boiling point of the perfluoropentane and at body temperature and thus has potential for use in vivo. Experiments involving the release of fluorescent calcein molecules were performed on eLiposomes to measure the release of calcein at various temperatures in the absence of ultrasound. Results showed that eLiposomes are stable …

Integrating The Public In Mosquito Management: Active Education By Community Peers Can Lead To Significant Reduction In Peridomestic Container Mosquito Habitats, Kristen Healy, George Hamilton, Taryn Crepeau, Sean Healy, Isik Unlu, Ary Farajollahi, Dina M. Fonseca

Faculty Publications

Mosquito species that utilize peridomestic containers for immature development are commonly aggressive human biters, and because they often reach high abundance, create significant nuisance. One of these species, the Asian tiger mosquito Aedes albopictus, is an important vector of emerging infectious diseases, such as dengue, chikungunya, and Zika fevers. Integrated mosquito management (IMM) of Ae. albopictus is particularly difficult because it requires access to private yards in urban and suburban residences. It has become apparent that in the event of a public health concern due to this species, homeowners will have to be active participants in the control process by …

Bringing The Congregations Back In: Religious Markets, Congregational Density, And American Religious Participation, Troy Blanchard, Samuel Stroope, Charles Tolbert

Faculty Publications

We draw on the organizational ecology tradition to frame the relationship between the religious environment of a community and local religious participation. Prior research linking religious environments to religious participation downplays a key organizational aspect of religion: the congregation. Following the organizational ecology usage of density, we argue that congregational density—the number of congregations per person within a community—impacts religious involvement by providing opportunities for participation and by fostering social accountability networks within congregations. Drawing on data from the 2000 Social Capital Community Benchmark Survey, we test the hypothesis that congregational density in a locality is associated with greater religious …

Responses Of Sympatric Karenia Brevis, Prorocentrum Minimum, And Heterosigma Akashiwo To The Exposure Of Crude Oil, Koray Özhan, Sibel Bargu

Faculty Publications

Impacts of the Deepwater Horizon oil spill on phytoplankton, particularly, the tolerability and changes to the toxin profiles of harmful toxic algal species remain unknown. The degree to which oil-affected sympatric Karenia brevis, Prorocentrum minimum, and Heterosigma akashiwo, all of which are ecologically important species in the Gulf of Mexico, was investigated. Comparison of their tolerability to that of non-toxic species showed that the toxin-production potential of harmful species does not provide a selective advantage. Investigated toxin profiles for K. brevis and P. minimum demonstrated an increase in toxin productivity at the lowest crude oil concentration (0.66 mg L^{−1 …}