Digital Commons Network^™

Single Family Zoning, Intimate Association, And The Right To Choose Household Companions, Rigel C. Oliveri

Faculty Publications

Many local governments use single family zoning ordinances to restrict occupancy in residential areas to households whose members are all related to one another by blood, marriage, or adoption. The Supreme Court upheld such ordinances in the 1974 case of Belle Terre v. Boraas, and they have been used to prevent all sort of groups from living together – from unmarried couples who are raising children to college students. This Article contends that Belle Terre is wholly incompatible with the Court’s modern jurisprudence on privacy and the right of intimate association. The case appears to have survived this long because …

Thriving In The Cold: Glacial Expansion And Post-Glacial Contraction Of A Temperate Terrestrial Salamander (Plethodon Serratus), Catherine E. Newman, Christopher C. Austin

Faculty Publications

© 2015 Newman, Austin. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The dynamic geologic history of the southeastern United States has played a major role in shaping the geographic distributions of amphibians in the region. In the phylogeographic literature, the predominant pattern of distribution shifts through time of temperate species is one of contraction during glacial maxima and persistence in refugia. However, the diverse biology and ecology of amphibian species suggest that a …

Phylogenetics, Divergence Times And Diversification From Three Genomic Partitions In Monocots, Kate L. Hertweck, Michael S. Kinney, Stephanie A. Stuart, Olivier Maurin, Sarah Mathews, Mark W. Chase, Maria A. Gandolfo, J. Chris Pires

Faculty Publications

© 2015 The Authors. Resolution of evolutionary relationships among some monocot orders remains problematic despite the application of various taxon and molecular locus sampling strategies. In this study we sequenced and analysed a fragment of the low-copy, nuclear phytochrome C (PHYC) gene and combined these data with a previous multigene data set (four plastid, one mitochondrial, two nuclear ribosomal loci) to determine if adding this marker improved resolution and support of relationships among major lineages of monocots. Our results indicate the addition of PHYC to the multigene dataset increases support along the backbone of the monocot tree, although relationships among …

Ocean Acidification Research In The 'Post-Genomic' Era: Roadmaps From The Purple Sea Urchin Strongylocentrotus Purpuratus, Tyler G. Evans, Jacqueline L. Padilla-Gamiño, Morgan W. Kelly, Melissa H. Pespeni, Francis Chan, Bruce A. Menge, Brian Gaylord, Tessa M. Hill, Ann D. Russell, Stephen R. Palumbi, Eric Sanford, Gretchen E. Hofmann

Faculty Publications

© 2015 Elsevier Inc. Advances in nucleic acid sequencing technology are removing obstacles that historically prevented use of genomics within ocean change biology. As one of the first marine calcifiers to have its genome sequenced, purple sea urchins (Strongylocentrotus purpuratus) have been the subject of early research exploring genomic responses to ocean acidification, work that points to future experiments and illustrates the value of expanding genomic resources to other marine organisms in this new 'post-genomic' era. This review presents case studies of S. purpuratus demonstrating the ability of genomic experiments to address major knowledge gaps within ocean acidification. Ocean acidification …

Vitrification Of Sperm From Marine Fish: Effect On Motility And Membrane Integrity, Rafael Cuevas-Uribe, Edward J. Chesney, Jonathan Daly, Terrence R. Tiersch

Faculty Publications

Our goal was to develop a standardized approach for sperm vitrification of marine fish that can be applied generally in aquatic species. The objectives were to: (i) estimate acute toxicity of cryoprotectants over a range of concentrations; (ii) evaluate the properties of vitrification solutions (VS); (iii) evaluate different thawing solutions and (iv) evaluate sperm quality after thawing by examination of motility and membrane integrity. Sperm were collected from red snapper (Lutjanus campechanus), spotted seatrout (Cynoscion nebulosus) and red drum (Sciaenops ocellatus). A total of 29 combinations of cryoprotectants were evaluated for toxicity and glass formation. Samples were loaded onto 10-μL …

Adventitious Root Primordia Formation And Development In Stem Nodes Of ‘Georgia Jet’ Sweetpotato, Ipomoea Batatas, Jun Ma, Roni Aloni, Arthur Villordon, Don Labonte, Yanir Kfir, Hanita Zemach, Amnon Schwartz, Leviah Althan, Nurit Firon

Faculty Publications

PREMISE OF THE STUDY: Yield in sweetpotato is determined by the number of storage roots produced per plant. Storage roots develop from adventitious roots (ARs) present in stem cuttings that serve as propagation material. Data on the origin of sweetpotato ARs and the effect of nodal position on AR establishment and further development are limited. METHODS: We anatomically described root primordium initiation using stem sections and measured number of root primordia formed at different nodal positions using light microscopy and correlated nodal positions with AR number and length 14 d after planting (DAP). KEY RESULTS: Primordia for ARs initiate at …

An Insight Into The Sialome Of The Lone Star Tick, Ambylomma Americanum, With A Glimpse On Its Time Dependent Gene Expression, Shahid Karim, José M.C. Ribeiro

Faculty Publications

Hard ticks feed for several days or weeks on their hosts. Blood feeding is assisted by tick saliva, which is injected in the host skin regularly, alternating with blood ingestion. Tick saliva contains hundreds or thousands of different peptides and other bioactive compounds that assist feeding by inhibiting their hosts’ blood clotting, platelet aggregation, vasoconstriction, as well as pain and itching. Immunomodulatory and antimicrobial peptides are also found in tick saliva. Molecular characterization of tick salivary compounds, or its sialome (from the Greek sialos = saliva), helps identification of possible antigens that might confer anti-tick immunity, as well as identifying …

Comment On "Fluorotechnology Is Critical To Modern Life: The Fluorocouncil Counterpoint To The Madrid Statement", Ian T. Cousins, Simona A. Balan, Martin Scheringer, Roland Weber, Zhanyun Wang, Arlene Blum, Miriam Diamond, Tony Fletcher, Gretta Goldenman, Christopher Higgins, Avery E. Lindeman, Graham Peaslee

Faculty Publications

No abstract provided.

Structural Variation Of Alpha-Synuclein With Temperature By A Coarse-Grained Approach With Knowledge-Based Interactions, Peter Mirau, Barry L. Farmer, Ras B. Pandey

Faculty Publications

Despite enormous efforts, our understanding the structure and dynamics of α-synuclein (ASN), a disordered protein (that plays a key role in neurodegenerative disease) is far from complete. In order to better understand sequence-structure-property relationships in α-SYNUCLEIN we have developed a coarse-grained model using knowledge-based residue-residue interactions and used it to study the structure of free ASN as a function of temperature (T) with a large-scale Monte Carlo simulation. Snapshots of the simulation and contour contact maps show changes in structure formation due to self-assembly as a function of temperature. Variations in the residue mobility profiles reveal clear distinction among three …

Discovering Self: Childbearing Adolescents' Maternal Identity, Janelle L. B. Macintosh, Lynn Clark Callister

Faculty Publications

Purpose: Adolescent pregnancy and motherhood have long been a topic of interest for many healthcare professionals. However, there are limited data on how childbearing adolescents incorporate motherhood identity into their sense of self. The purpose of this study was to explore how childbearing adolescents perceive motherhood as becoming part of their personal identity.

Study Design: This qualitative study using ethnographic data collection involved 7 months of observation, interaction, and interviews.

Methods: Data were collected from nine expectant adolescents during in-depth interviews. All participants were patients at a teen mother and child clinic staffed by certified nurse midwives and a pediatrician. …

Dynamic Environmentalism And Adaptive Management: Legal Obstacles And Opportunities, Jonathan H. Adler

Faculty Publications

Noted ecologist Daniel Botkin argues that "solving our environmental problems requires a new perspective" of environmental concerns that incorporates contemporary scientific understandings and embraces humanity's role in environmental management. Recognizing a new perspective is but the first step, however. There is also a need to identify how this perspective can inform environmental policy, not just on the ground but in the very institutional architecture of environmental law and management. Then comes the really hard part, for even if it is possible to conceive of how environmental management should proceed, it may be devilishly difficult to put such ideas into practice. …

Inferring Pathway Dysregulation In Cancers From Multiple Types Of Omic Data, Shelley M. Macneil, William E. Johnson, Dean Y. Li, Stephen R. Piccolo, Andrea H. Bild

Faculty Publications

Although in some cases individual genomic aberrations may drive disease development in isolation, a complex interplay among multiple aberrations is common. Accordingly, we developed Gene Set Omic Analysis (GSOA), a bioinformatics tool that can evaluate multiple types and combinations of omic data at the pathway level. GSOA uses machine learning to identify dysregulated pathways and improves upon other methods because of its ability to decipher complex, multigene patterns. We compare GSOA to alternative methods and demonstrate its ability to identify pathways known to play a role in various cancer phenotypes. Software implementing the GSOA method is freely available from https://bitbucket.org/srp33/gsoa …

Experimental Support For A Single Electron-Transfer Oxidation Mechanism In Firefly Bioluminescence, Bruce R. Branchini, Curran E. Behney, Tara L. Southworth, Danielle M. Fontaine, Andrew M. Gulick, David J. Vinyard, Gary W. Brudvig

Faculty Publications

© 2015 American Chemical Society. Firefly luciferase produces light by converting substrate beetle luciferin into the corresponding adenylate that it subsequently oxidizes to oxyluciferin, the emitter of bioluminescence. We have confirmed the generally held notions that the oxidation step is initiated by formation of a carbanion intermediate and that a hydroperoxide (anion) is involved. Additionally, structural evidence is presented that accounts for the delivery of oxygen to the substrate reaction site. Herein, we report key convincing spectroscopic evidence of the participation of superoxide anion in a related chemical model reaction that supports a single electron-transfer pathway for the critical oxidative …

Structural Analysis Of Substrate, Reaction Intermediate, And Product Binding In Haemophilus Influenzae Biotin Carboxylase, Tyler C. Broussard, Svetlana Pakhomova, David B. Neau, Ross Bonnot, Grover L. Waldrop

Faculty Publications

© 2015 American Chemical Society. Acetyl-CoA carboxylase catalyzes the first and regulated step in fatty acid synthesis. In most Gram-negative and Gram-positive bacteria, the enzyme is composed of three proteins: biotin carboxylase, a biotin carboxyl carrier protein (BCCP), and carboxyltransferase. The reaction mechanism involves two half-reactions with biotin carboxylase catalyzing the ATP-dependent carboxylation of biotin-BCCP in the first reaction. In the second reaction, carboxyltransferase catalyzes the transfer of the carboxyl group from biotin-BCCP to acetyl-CoA to form malonyl-CoA. In this report, high-resolution crystal structures of biotin carboxylase from Haemophilus influenzae were determined with bicarbonate, the ATP analogue AMPPCP; the carboxyphosphate …

A Simple And Fast Physics-Based Analytical Method To Calculate Therapeutic And Stray Doses From External Beam, Megavoltage X-Ray Therapy, Lydia J. Jagetic, Wayne D. Newhauser

Faculty Publications

State-of-the-art radiotherapy treatment planning systems provide reliable estimates of the therapeutic radiation but are known to underestimate or neglect the stray radiation exposures. Most commonly, stray radiation exposures are reconstructed using empirical formulas or lookup tables. The purpose of this study was to develop the basic physics of a model capable of calculating the total absorbed dose both inside and outside of the therapeutic radiation beam for external beam photon therapy. The model was developed using measurements of total absorbed dose in a water-box phantom from a 6 MV medical linear accelerator to calculate dose profiles in both the in-plane …

The Ocean Sampling Day Consortium, Anna Kopf, Mesude Bicak, Renzo Kottman, Julia Schnetzer, Ivaylo Kostadinov, Katja Lehmann, Antonio Fernandez-Guerra, Christian Jeanthon, Eyal Rahav, Matthias Ulrich, Antje Wichels, Gunnar Gerdts, Paraskevi Polymenakou, Giorgos Kotoulas, Rania Siam, Rehab Z. Abdallah, Eva C. Sonnenschein, Thierry Cariou, Fergal O'Gara, Stephen Jackson, Sandi Orlic, Michael Steinke, Julia Busch, Bernardo Duarte, Isabel Caçador, João Canning-Clode, Oleksandra Bobrova, Viggo Marteinsson, Eyjolfur Reynisson, Clara Magalhães Loureiro, Gian Marco Luna, Grazia Marina Quero, Carolin R. Löscher, Anke Kremp, Marie E. Delorenzo, Lise Øvreås, Jennifer Tolman, Julie Laroche, Antonella Penna, Marc Frischer, Timothy Davis, Barker Katherine, Christopher P. Meyer, Sandra Ramos, Catarina Magalhães, Florence Jude-Lemeilleur, Ma Leopoldina Aguirre-Macedo, Shiao Y. Wang, Nicole Poulton, Scott Jones, Rachel Collin, Jed A. Fuhrman, Pascal Conan, Cecilia Alonso, Noga Stambler, Kelly Goodwin, Michael M. Yakimov, Federico Baltar, Levente Bodrossy, Jodie Van De Kamp, Dion Mf Frampton, Martin Ostrowski, Paul Van Ruth, Paul Malthouse, Simon Claus, Klaas Deneudt, Jonas Mortelmans, Sophie Pitois, David Wallom, Ian Salter, Rodrigo Costa, Declan C. Schroeder, Mahrous M. Kandil, Valentina Amaral, Florencia Biancalana, Rafael Santana, Maria Luiza Pedrotti, Takashi Yoshida, Hiroyuki Ogata, Tim Ingleton, Kate Munnik, Naiara Rodriguez-Ezpeleta, Veronique Berteaux-Lecellier, Patricia Wecker, Ibon Cancio, Daniel Valout, Christina Bienhold, Hassan Ghazal, Bouchra Chaouni, Soumya Essayeh, Sara Ettamimi, El Houcine Zaid, Noureddine Boukhatem, Abderrahim Bouali, Rajaa Chahboune, Said Barrijal, Mohammed Timinouni, Fatima El Otmani, Mohamed Bennani, Marianna Mea, Nadezhda Todorova, Ventzislav Karamfilov, Petra Ten Hoopten, Guy Cochrane, Stephanie L'Haridon, Kemal Can Bizel, Alessandro Vezzi, Federico M. Lauro, Patrick Martin, Rachelle M. Jensen, Jamie Hinks, Susan Gebbels, Riccardo Rosselli, Fabio De Pascale, Riccardo Schiavon, Antonina Dos Santos, Emilie Villar, Stéphane Pesant, Bruno Cataletto, Francesca Malfatti, Ranjith Edirisinghe, Jorge A. Herrera Silveira, Michele Barbier, Valentina Turk, Tinkara Tinta, Wayne J. Fuller, Ilkay Salihoglu, Nedime Serakinci, Mahmut Cerkez Ergoren, Eileen Bresnan, Juan Iriberri, Paul Anders Fronth Nyhus, Edvardsen Bente, Hans Erik Karlsen, Peter N. Golyshin, Josep M. Gasol, Snejana Moncheva, Nina Dzhembekova, Zackary Johnson, Christopher Daivd Sinigalliano, Maribeth Louise Gidley, Adriana Zingone, Roberto Danovaro, George Tsiamis, Melody S. Clark, Ana Cristina Costa, Monia El Bour, Ana M. Martins, R. Eric Collins, Anne-Lise Ducluzeau, Jonathan Martinez, Mark J. Costello, Linda A. Amaral-Zettler, Jack A. Gilbert, Neil Davies, Dawn Field, Frank Oliver Glöckner

Faculty Publications

Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world’s oceans. It is a simultaneous global mega-sequencing campaign aiming to generate the largest standardized microbial data set in a single day. This will be achievable only through the coordinated efforts of an Ocean Sampling Day Consortium, supportive partnerships and networks between sites. This commentary outlines the establishment, function and aims of the Consortium and describes our vision for a sustainable study of marine microbial communities and their embedded functional traits.

The Geographic Distribution Of A Tropical Montane Bird Is Limited By A Tree: Acorn Woodpeckers (Melanerpes Formicivorus) And Colombian Oaks (Quercus Humboldtii) In The Northern Andes, Benjamin G. Freeman, Nicholas A. Mason

Faculty Publications

© 2015 Freeman, Mason. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Species distributions are limited by a complex array of abiotic and biotic factors. In general, abiotic (climatic) factors are thought to explain species' broad geographic distributions, while biotic factors regulate species' abundance patterns at local scales. We used species distribution models to test the hypothesis that a biotic interaction with a tree, the Colombian oak (Quercus humboldtii), limits the broad-scale distribution of …

Living On The Edge: Settlement Patterns By The Symbiotic Barnacle Xenobalanus Globicipitis On Small Cetaceans, Juan M. Carillo, Robin M. Overstreet, Juan A. Raga, Francisco J. Aznar

Faculty Publications

The highly specialized coronulid barnacle Xenobalanus globicipitis attaches exclusively on cetaceans worldwide, but little is known about the factors that drive the microhabitat patterns on its hosts. We investigate this issue based on data on occurrence, abundance, distribution, orientation, and size of X. globicipitis collected from 242 striped dolphins (Stenella coeruleoalba) that were stranded along the Mediterranean coast of Spain. Barnacles exclusively infested the fins, particularly along the trailing edge. Occurrence, abundance, and density of X. globicipitis were significantly higher, and barnacles were significantly larger, on the caudal fin than on the flippers and dorsal fin. …

Correction: Biological Evidence Supports An Early And Complex Emergence Of The Isthmus Of Panama (Proceedings Of National Academy Of Sciences United States Of America (2015), 112 (6110-6115) Doi: 10.1073/Pnas.1423853112), Christine D. Bacon, Daniele Silvestro, Carlos Jaramillo, Brian Tilston Smith, Prosanta Chakrabarty, Alexandre Antonelli

Faculty Publications

No abstract provided.

When Nanoparticles Meet Biofilms—Interactions Guiding The Environmental Fate And Accumulation Of Nanoparticles, Kaoru Ikuma, Alan W. Decho, Boris T. Lau

Faculty Publications

Bacteria are essential components of all natural and many engineered systems. The most active fractions of bacteria are now recognized to occur as biofilms, where cells are attached and surrounded by a secreted matrix of “sticky” extracellular polymeric substances. Recent investigations have established that significant accumulation of nanoparticles (NPs) occurs in aquatic biofilms. These studies point to the emerging roles of biofilms for influencing partitioning and possibly transformations of NPs in both natural and engineered systems. While attached biofilms are efficient “sponges” for NPs, efforts to elucidate the fundamental mechanisms guiding interactions between NPs and biofilms have just begun. In …

The Identification Of Novel Protein-Protein Interactions In Liver That Affect Glucagon Receptor Activity, Junfeng Han, Ming Zhang, Sean Froese, Feihan F. Dai, Mélanie Robitaille, Alpana Bhattacharjee, Xinyu Huang, Weiping Jia, Stéphane Angers, Michael B. Wheeler, Li Wei

Faculty Publications

Glucagon regulates glucose homeostasis by controlling glycogenolysis and gluconeogenesis in the liver. Exaggerated and dysregulated glucagon secretion can exacerbate hyperglycemia contributing to type 2 diabetes (T2D). Thus, it is important to understand how glucagon receptor (GCGR) activity and signaling is controlled in hepatocytes. To better understand this, we sought to identify proteins that interact with the GCGR to affect ligand-dependent receptor activation. A Flag-tagged human GCGR was recombinantly expressed in Chinese hamster ovary (CHO) cells, and GCGR complexes were isolated by affinity purification (AP). Complexes were then analyzed by mass spectrometry (MS), and protein-GCGR interactions were validated by co-immunoprecipitation (Co-IP) …

Resveratrol Induces Brown-Like Adipocyte Formation In White Fat Through Activation Of Amp-Activated Protein Kinase (Ampk) Α1, S. Wang, X. Liang, Q. Yang, X. Fu, C. J. Rogers, M. Zhu, B. D. Rodgers, Q. Jiang, M. V. Dodson, M. Du

Faculty Publications

© 2015 Macmillan Publishers Limited. All rights reserved. Objective: Development of brown-like/beige adipocytes in white adipose tissue (WAT) helps to reduce obesity. Thus we investigated the effects of resveratrol, a dietary polyphenol capable of preventing obesity and related complications in humans and animal models, on brown-like adipocyte formation in inguinal WAT (iWAT). Methods: CD1 female mice (5-month old) were fed a high-fat diet with/without 0.1% resveratrol. In addition, primary stromal vascular cells separated from iWAT were subjected to resveratrol treatment. Markers of brown-like (beige) adipogenesis were measured and the involvement of AMP-activated protein kinase (AMPK) α1 was assessed using conditional …

A Comprehensive Characterization Of The 70 Virginis Planetary System, Stephen R. Kane, Tabetha S. Boyajian, Gregory W. Henry, Y. Katherina Feng, Natalie R. Hinkel, Debra A. Fischer, Kaspar Von Braun, Andrew W. Howard, Jason T. Wright

Faculty Publications

An on-going effort in the characterization of exoplanetary systems is the accurate determination of host star properties. This effort extends to the relatively bright host stars of planets discovered with the radial velocity method. The Transit Ephemeris Refinement and Monitoring Survey (TERMS) is aiding in these efforts as part of its observational campaign for exoplanet host stars. One of the first known systems is that of 70 Virginis, which harbors a jovian planet in an eccentric orbit. Here we present a complete characterization of this system with a compilation of TERMS photometry, spectroscopy, and interferometry. We provide fundamental properties of …

Nitric Oxide Releases Cl− From Acidic Organelles In Retinal Amacrine Cells, Vijai Krishnan, Evanna Gleason

Faculty Publications

© 2015 Krishnan and Gleason Determining the factors regulating cytosolic Cl− in neurons is fundamental to our understanding of the function of GABA- and glycinergic synapses. This is because the Cl− distribution across the postsynaptic plasma membrane determines the sign and strength of postsynaptic voltage responses. We have previously demonstrated that nitric oxide (NO) releases Cl− into the cytosol from an internal compartment in both retinal amacrine cells and hippocampal neurons. Furthermore, we have shown that the increase in cytosolic Cl− is dependent upon a decrease in cytosolic pH. Here, our goals were to confirm the compartmental nature of the …

Na+/K+-Atpase α1 Mrna Expression In The Gill And Rectal Gland Of The Atlantic Stingray, Dasyatis Sabina, Following Acclimation To Increased Salinity Physiology, Andrew N. Evans, Faith N. Lambert

Faculty Publications

Background: The salt-secreting rectal gland plays a major role in elasmobranch osmoregulation, facilitating ion balance in hyperosmotic environments in a manner analogous to the teleost gill. Several studies have examined the central role of the sodium pump Na⁺/K⁺-ATPase in osmoregulatory tissues of euryhaline elasmobranch species, including regulation of Na⁺/K⁺-ATPase activity and abundance in response to salinity acclimation. However, while the transcriptional regulation of Na⁺/K⁺-ATPase in the teleost gill has been well documented the potential for mRNA regulation to facilitate rectal gland plasticity during salinity acclimation in elasmobranchs …

Incorporation Of Iodine Into Apatite Structure: A Crystal Chemistry Approach Using Artificial Neural Network, Jianwei Wang

Faculty Publications

© 2015 Wang. Materials with apatite crystal structure have a great potential for incorporating the long-lived radioactive iodine isotope (129 I) in the form of iodide (I−) from nuclear waste streams. Because of its durability and potentially high iodine content, the apatite waste form can reduce iodine release rate and minimize the waste volume. Crystal structure and composition of apatite (A5 (XO4)3 Z) was investigated for iodide incorporation into the channel of the structure using Artificial Neural Network. A total of 86 experimentally determined apatite crystal structures of different compositions were compiled from literature, and 44 of them were used …

Investigating The Paleoecological Consequences Of Supercontinent Breakup: Sponges Clean Up In The Early Jurassic, Frank Corsetti, Kathleen Ritterbush, David Bottjer, Sarah Greene, Yadira Ibarra, Joyce Yager, A. Joshua West, William Berelson, Silvia Rosas, Thorsten Becker, Naomi Levine, Sean Loyd, Rowan Martindale, Victoria Petryshyn, Nathan Carroll, Elizabeth Petsios, Olivia Piazza, Carlie Pietsch, Jessica Stellmann, Jeffrey Thompson, Kirstin Washington, Dylan Wilmeth

Faculty Publications

The continued release of fossil fuel carbon into the atmosphere today means it is imperative to understand Earth system response to CO2 rise, and the geologic record offers unique opportunities to investigate such behavior. Stomatal and paleosol proxies demonstrate a large change in atmospheric pCO2 across the Triassic-Jurassic (T-J) transition, concomitant with the eruption and emplacement of the Central Atlantic Magmatic Province (CAMP) and the splitting of Pangea. As one of the “big 5” mass extinctions—when the so-called modern fauna was particularly hard hit—we know the biosphere was severely affected during this time, but the details are relatively poorly understood, …

Directional Sound Sensitivity In Utricular Afferents In The Toadfish Opsanus Tau, Karen P. Maruska, Allen F. Mensinger

Faculty Publications

© 2015 Published by The Company of Biologists Ltd. The inner ear of fishes contains three paired otolithic end organs, the saccule, lagena and utricle, which function as biological accelerometers. The saccule is the largest otolith in most fishes and much of our current understanding on auditory function in this diverse group of vertebrates is derived from anatomical and neurophysiological studies on this end organ. In contrast, less is known about how the utricle contributes to auditory functions. In this study, chronically implanted electrodes were used, along with neural telemetry or tethers to record primary afferent responses from the utricular …

Ucp1 Is An Essential Mediator Of The Effects Of Methionine Restriction On Energy Balance But Not Insulin Sensitivity, Desiree Wanders, David H. Burk, Cory C. Cortez, Nancy T. Van, Kirsten P. Stone, Mollye Baker, Tamra Mendoza, Randall L. Mynatt, Thomas W. Gettys

Faculty Publications

© FASEB. Dietary methionine restriction (MR) by 80%increases energy expenditure (EE), reduces adiposity, and improves insulin sensitivity. We propose that the MRinduced increase in EE limits fat deposition by increasing sympathetic nervous system-dependent remodeling of white adipose tissue and increasing uncoupling protein 1 (UCP1) expression in both white and brown adipose tissue. In independent assessments of the role of UCP1 as a mediator of MR's effects on EE and insulin sensitivity, EE did not differ between wild-type (WT) and Ucp1^-/- mice on the control diet, butMR increased EE by 31%and reduced adiposity by 25% in WT mice. In contrast, …

Accelerating The Pace Of Protein Functional Annotation With Intel Xeon Phi Coprocessors, Wei P. Feinstein, Juana Moreno, Mark Jarrell, Michal Brylinski

Faculty Publications

© 2002-2011 IEEE. Intel Xeon Phi is a new addition to the family of powerful parallel accelerators. The range of its potential applications in computationally driven research is broad; however, at present, the repository of scientific codes is still relatively limited. In this study, we describe the development and benchmarking of a parallel version of {\mmb e}FindSite, a structural bioinformatics algorithm for the prediction of ligand-binding sites in proteins. Implemented for the Intel Xeon Phi platform, the parallelization of the structure alignment portion of {\mmb e}FindSite using pragma-based OpenMP brings about the desired performance improvements, which scale well with the …