Digital Commons Network^™

Mechanisms Of Animal Diapause: Recent Developments From Nematodes, Crustaceans, Insects, And Fish, Steven C. Hand, David L. Denlinger, Jason E. Podrabsky, Richard Roy

Faculty Publications

© 2016 the American Physiological Society. Life cycle delays are beneficial for opportunistic species encountering suboptimal environments. Many animals display a programmed arrest of development (diapause) at some stage(s) of their development, and the diapause state may or may not be associated with some degree of metabolic depression. In this review, we will evaluate current advancements in our understanding of the mechanisms responsible for the remarkable phenotype, as well as environmental cues that signal entry and termination of the state. The developmental stage at which diapause occurs dictates and constrains the mechanisms governing diapause. Considerable progress has been made in …

The Abiotic And Biotic Drivers Of Rapid Diversification In Andean Bellflowers (Campanulaceae), Laura P. Lagomarsino, Fabien L. Condamine, Alexandre Antonelli, Andreas Mulch, Charles C. Davis

Faculty Publications

© 2016 New Phytologist Trust. The tropical Andes of South America, the world's richest biodiversity hotspot, are home to many rapid radiations. While geological, climatic, and ecological processes collectively explain such radiations, their relative contributions are seldom examined within a single clade. We explore the contribution of these factors by applying a series of diversification models that incorporate mountain building, climate change, and trait evolution to the first dated phylogeny of Andean bellflowers (Campanulaceae: Lobelioideae). Our framework is novel for its direct incorporation of geological data on Andean uplift into a macroevolutionary model. We show that speciation and extinction are …

Addressing Emerging Risks: Scientific And Regulatory Challenges Associated With Environmentally Persistent Free Radicals, Tammy R. Dugas, Slawomir Lomnicki, Stephania A. Cormier, Barry Dellinger, Margaret Reams

Faculty Publications

© 2016 by the authors; licensee MDPI, Basel, Switzerland. Airborne fine and ultrafine particulate matter (PM) are often generated through widely-used thermal processes such as the combustion of fuels or the thermal decomposition of waste. Residents near Superfund sites are exposed to PM through the inhalation of windblown dust, ingestion of soil and sediments, and inhalation of emissions from the on-site thermal treatment of contaminated soils. Epidemiological evidence supports a link between exposure to airborne PM and an increased risk of cardiovascular and pulmonary diseases. It is well-known that during combustion processes, incomplete combustion can lead to the production of …

Vector Species Richness Increases Haemorrhagic Disease Prevalence Through Functional Diversity Modulating The Duration Of Seasonal Transmission, Andrew W. Park, Christopher A. Cleveland, Tad A. Dallas, Joseph L. Corn

Faculty Publications

© Copyright Cambridge University Press 2015. SUMMARY Although many parasites are transmitted between hosts by a suite of arthropod vectors, the impact of vector biodiversity on parasite transmission is poorly understood. Positive relationships between host infection prevalence and vector species richness (SR) may operate through multiple mechanisms, including (i) increased vector abundance, (ii) a sampling effect in which species of high vectorial capacity are more likely to occur in species-rich communities, and (iii) functional diversity whereby communities comprised species with distinct phenologies may extend the duration of seasonal transmission. Teasing such mechanisms apart is impeded by a lack of appropriate …

A Generalized Reed Model With Application To Wildfire Risk In Even-Aged Southern United States Pine Plantations, Andres Susaeta, Douglas R. Carter, Sun Joseph Chang, Damian C. Adams

Faculty Publications

We develop a generalized Reed model to incorporate the risk of wildfires on optimal management of slash pine (Pinus elliotti var. elliotti) in the southern United States. Comparative static analyses are conducted to determine the impacts of the probability of increasing and constant wildfire risk as forest stand ages, and portion of stand that is salvageable following a wildfire, on slash pine harvest decisions. Our results reveal that increasing the current risk of wildfire damage would shorten the current optimal slash pine harvest age, while increasing the future risk of wildfire losses would lengthen the current optimal harvest age. We …

Cancer Stem-Like Cells Accumulated In Nickel-Induced Malignant Transformation, Lei Wang, Jia Fan, John Andrew Hitron, Young Ok Son, James T.F. Wise, Ram Vinod Roy, Donghern Kim, Jin Dai, Poyil Pratheeshkumar, Zhuo Zhang, Xianglin Shi

Faculty Publications

Nickel compounds are known as human carcinogens. Chronic environmental exposure to nickel is a worldwide health concern. Although the mechanisms of nickel-induced carcinogenesis are not well understood, recent studies suggest that stem cells/cancer stem cells are likely important targets. This study examines the role of cancer stem cells in nickelinduced cell transformation. The nontransformed human bronchial epithelial cell line (Beas-2B) was chronically exposed to nickel chloride for 12 months to induce cell transformation. Nickel induced Beas-2B cell transformation, and cancer stemlike cells were enriched in nickel-transformed cell (BNiT) population. The BNiT cancer stem-like cells demonstrated enhanced self-renewal and distinctive differentiation …

What Time Is It? Deep Learning Approaches For Circadian Rhythms, Forest Agostinelli, Nicholas Ceglia, Babak Shahbaba, Paolo Sassone-Corsi, Pierre Baldi

Faculty Publications

Motivation: Circadian rhythms date back to the origins of life, are found in virtually every species and every cell, and play fundamental roles in functions ranging from metabolism to cognition. Modern high-throughput technologies allow the measurement of concentrations of transcripts, metabolites and other species along the circadian cycle creating novel computational challenges and opportunities, including the problems of inferring whether a given species oscillate in circadian fashion or not, and inferring the time at which a set of measurements was taken.

Results: We first curate several large synthetic and biological time series datasets containing labels for both periodic and aperiodic …

Effects Of Hypoxia And Elevated Ammonia Concentration On The Viability Of Red Snapper Embryos And Early Larvae, Agnes Bardon-Albaret, Eric A. Saillant

Faculty Publications

The effects of hypoxic conditions and elevated ammonia concentrations on the viability of embryos and newly hatched larvae of the red snapper (Lutjanus campechanus) were investigated. In all experiments, tested levels of hypoxia or ammonia concentrationswere applied to embryos and unfed newly hatched larvae from three different spawns. Exposures began at 1 h post fertilization (pf) and lasted until all individuals in a group had expired. Survival rates were monitored daily in duplicates for each spawn in each treatment. Fertilized eggs exposed to 2 mg L-1 dissolved oxygen (29% saturation) showed complete mortality before hatch while 81% of embryos in …

Larval Fishes Utilize Batesian Mimicry As A Survival Strategy In The Plankton, Adam T. Greer, C. Brock Woodson, Cedric M. Guigand, Robert K. Cowan

Faculty Publications

Marine teleost fishes often experience over 99% mortality in the early life stages (eggs and larvae), yet larval survival is essential to population sustainability. Marine fish larvae from a wide range of families display elaborate, delicate features that bear little resemblance to adult forms and hinder their swimming escape ability by increasing drag. Here, we systematically examine the criteria needed for Batesian mimicry to evolve as a survival strategy and present new evidence from in situ imaging technology and simulation modelling to support the hypothesis that many larval morphological features (particularly long, delicate fin rays) and behaviors evolved at least …

Controlled Thiol-Ene Polymer Microsphere Production Using A Low-Frequency Acoustic Excitation Coaxial Flow Method, Amber D. Windham, Patrick M. Lowe, Keith W. Conley, Anton D. Netchaev, Randy K. Buchanan, J. Paige Buchanan

Faculty Publications

A novel technique for the production of thiol-ene microspheres using acoustic resonance and coaxial flow is reported. The method utilizes low-frequency acoustically driven mechanical perturbations to disrupt the flow of a thiol-ene liquid jet, resulting in small thiol-ene droplets that are photochemically polymerized to yield thiol-ene microspheres. Tuning of the frequency, amplitude, and monomer solution viscosity are critical parameters impacting the diameter of the microspheres produced. Characterization by optical microscopy, scanning electron microscopy, and dynamic light scattering reveal microspheres of diametersm, with narrow particle distributions. (C) 2016 Elsevier Ltd. All rights reserved.

Pubertal Maturation Compression And Behavioral Impulsivity Among Boys At Increased Risk For Substance Use, Charles W. Mathias, Nora E. Charles, Yuanyuan Lang, Ashley Acheson, Sarah L. Lake, Stacy R. Ryan, Rene L. Olvera, Donald M. Dougherty

Faculty Publications

Objectives: While early onset of puberty among girls has been related to substance use involvement and other adverse outcomes, less research has examined pubertal development and outcomes in boys. Further, research on puberty has not been conducted in the context of other risk factors for substance use involvement such as impulsivity. To address these gaps, this study characterized boys' pubertal development from preadolescence to mid adolescence and related it to substance use risk and behavioral impulsivity.

Methods: A sample of 153 boys completed the Pubertal Development Scale to assess perception of their pubertal development relative to same-age peers …

Design Of Compact Mach-Zehnder Interferometer-Based Slow-Light-Enhanced Plasmonic Waveguide Sensors, Yin Huang, Changjun Min, Shaohua Tao, Georgios Veronis

Faculty Publications

We investigate slow-light-enhanced ultracompact plasmonic Mach-Zehnder interferometer sensors. The sensing arm consists of either a conventional metal-dielectric-metal plasmonic waveguide or a waveguide system based on a plasmonic analogue of electromagnetically induced transparency. Such plasmonic electromagnetically induced transparency waveguide systems can be engineered to support slow-light modes. We find that, as the slowdown factor increases, the sensitivity of the effective index of the mode to variations of the refractive index of the material filling the structures increases. Such slow-light enhancements of the sensitivity to refractive index variations lead to enhanced sensing performance. We show that the Mach-Zehnder interferometer sensor using the …

A Critical Comparison Of Pyrolysis Of Cellulose, Lignin, And Pine Sawdust Using An Induction Heating Reactor, Pranjali D. Muley, Charles Henkel, Kamran K. Abdollahi, Cosmin Marculescu, Dorin Boldor

Faculty Publications

Fast pyrolysis of pinewood sawdust and two of its major components, namely lignin and cellulose was carried out using a laboratory scale induction-heating reactor. The effect of five different temperatures (500, 550, 600, 650 and 700 °C) was tested on the product yield and quality. The products were characterized to evaluate the water content, elemental composition, chemical composition and energy content. The char yield decreased with temperature for all of the biomasses. The maximum liquid yield of 55.28% was achieved at 600 °C for pine sawdust, and the highest liquid yields for cellulose and lignin were obtained at 500 °C. …

Sustainability Science Graduate Students As Boundary Spanners, Spencer R. Meyer, Vanessa R. Levesque, Karen H. Bieluch, Michelle L. Johnson, Bridie Mcgreavy, Stacia Dreyer, Hollie Smith

Faculty Publications

Graduate training in sustainability science (SS) focuses on interdisciplinary research, stakeholder-researcher partnerships, and creating solutions from knowledge. But becoming a sustainability scientist also requires specialized training that addresses the complex boundaries implicit in sustainability science approaches to solving social-ecological system challenges. Using boundary spanning as a framework, we use a case study of the Sustainability Solutions Initiative (SSI) at the University of Maine to explicate key elements for graduate education training in SS. We used a mixed-methods approach, including a quantitative survey and autoethnographic reflection, to analyze our experiences as SSI doctoral students. Through this research, we identified four essential …

Self-Forgiveness And Forgiveness-Seeking In Response To Rumination: Cardiac And Emotional Responses Of Transgressors, Sérgio P. Da Silva, Charlotte Vanoyen-Witvliet, Blake Riek

Faculty Publications

Self-forgiveness and forgiveness-seeking are important and understudied aspects of forgiveness. We examined the cardiac and emotional patterns of healthy young adults (40 women, 40 men) who recalled an unresolved offense they had caused another person. Participants engaged in four imagery conditions: ruminating about the offense, being humbly repentant and engaging in self-forgiveness, seeking forgiveness from the victim and receiving forgiveness, and seeking forgiveness from the victim and being begrudged. Being repentant and begrudged forgiveness by one’s victim was associated with the same level of guilt as when ruminating, but significantly more negative emotion, less control, and less empathy than when …

Feeding Ecology Of Three Coastal Shark Species In The Northwest Gulf Of Mexico, Jeffrey D. Plumlee, R. J.David Wells

Faculty Publications

The feeding ecology of 3 coastal shark species-Atlantic sharpnose Rhizoprionodon terraenovae, bonnethead Sphyrna tiburo, and blacktip Carcharhinus limbatus shark-was examined in the northwest Gulf of Mexico (GOM). A total of 601 sharks (305 Atlantic sharpnose, 239 bonnethead, and 57 blacktip) were collected over 2 yr from recreational anglers in Galveston, TX. All individuals had stomach contents examined and a subset (50 Atlantic sharpnose, 50 bonnethead, and 36 blacktip sharks) was analyzed for stable isotopes (carbon, nitrogen, and sulfur) in muscle tissue, revealing short-term and long-term feeding strategies. Both blacktip and Atlantic sharpnose shark stomach contents consisted of teleost fishes with …

Turning Contention Into Collaboration: Engaging Power, Trust, And Learning In Collaborative Networks, Vanessa R. Levesque, Aram J. K. Calhoun, Kathleen P. Bell, Teresa R. Johnson

Faculty Publications

Given the complexity and multiplicity of goals in natural resource governance, it is not surprising that policy debates are often characterized by contention and competition. Yet at times adversaries join together to collaborate to find creative solutions not easily achieved in polarizing forums. We employed qualitative interviews and a quantitative network analysis to investigate a collaborative network that formed to develop a resolution to a challenging natural resource management problem, the conservation of vernal pools. We found that power had become distributed among members, trust had formed across core interests, and social learning had resulted in shared understanding and joint …

Rickettsia Parkeri Colonization In Ambylomma Maculatum: The Role Of Superoxide Dismutases, Gary Crispell, Khemraj Budachetri, Shahid Karim

Faculty Publications

Background

The Gulf Coast tick (Amblyomma maculatum) is an arthropod vector of Rickettsia parkeri, the causative agent of American boutonneuse fever and an infectious agent of public health significance. In this study, we evaluated the biological significance of the superoxide dismutases (SODs) of A. maculatum in hematophagy and R. parkeri colonization within the tick host.

Methods

An RNA interference approach was used to measure the functional roles of tick SODs (Cu/Zn-SOD and Mn-SOD) in R. parkeri colonization of the tick vector. Total microbial load, R. parkeri infection rate, and compensatory mechanisms by tick genes were …

Genetic Manipulation Of Putrescine Biosynthesis Reprograms The Cellular Transcriptome And The Metabolome., Andrew Francis Page, Leland J. Cseke, Rakesh Minocha, Swathi A. Turlapati, Gopi K. Podila, Alexander V. Ulanov, Zhong Li, Subhash C. Minocha

Faculty Publications

With the increasing interest in metabolic engineering of plants using genetic manipulation and gene editing technologies to enhance growth, nutritional value and environmental adaptation, a major concern is the potential of undesirable broad and distant effects of manipulating the target gene or metabolic step in the resulting plant. A comprehensive transcriptomic and metabolomic analysis of the product may shed some useful light in this regard. The present study used these two techniques with plant cell cultures to analyze the effects of genetic manipulation of a single step in the biosynthesis of polyamines because of their well-known roles in plant growth, …

The Mechanosensory Lateral Line System Mediates Activation Of Socially-Relevant Brain Regions During Territorial Interactions, Julie M. Butler, Karen P. Maruska

Faculty Publications

© 2016 Butler and Maruska. Animals use multiple senses during social interactions and must integrate this information in the brain to make context-dependent behavioral decisions. For fishes, the largest group ofvertebrates, the mechanosensory lateral line system provides crucial hydrodynamic information for survival behaviors, but little is known about its function in social communication. Our previous work using the African cichlid fish, Astatotilapia burtoni, provided the first empirical evidence that fish use their lateral line system to detect water movements from conspecifics for mutual assessment and behavioral choices. It is unknown, however, where this socially-relevant mechanosensory information is processed in the …

A Randomized Controlled Trial Of An Integrated Brain, Body, And Social Intervention For Children With Adhd, Stephanie D. Smith, Lawrence A. Vitulano, Liliya Katsovich, Shuaixing Li, Christina Moore, Fenghua Li, Heidi Grantz, Xixi Zheng, Virginia Eicher, Selin Aktan Guloksuz, Yi Zheng, Jinxia Dong, Denis G. Sukhodolsky, James F. Leckman

Faculty Publications

Objective: This study evaluated the efficacy of an Integrated Brain, Body, and Social (IBBS) intervention for children with ADHD. Treatment consisted of computerized cognitive remediation training, physical exercises, and a behavior management strategy.

Method: Ninety-two children aged 5 to 9 years with ADHD were randomly assigned to 15 weeks of IBBS or to treatment-as-usual. Primary outcome measures included blinded clinician ratings of ADHD symptoms and global clinical functioning. Secondary outcome measures consisted of parent and teacher ratings of ADHD and neurocognitive tests.

Results: No significant treatment effects were found on any of our primary outcome measures. In terms of secondary …

Insights From The Pollination Drop Proteome And The Ovule Transcriptome Of Cephalotaxus At The Time Of Pollination Drop Production, Cary Pirone-Davies, Natalie Prior, Patrick Von Aderkas, Derek Smith, Darryl Hardie, William E. Friedman, Sarah Mathews

Faculty Publications

© The Author 2016. Published by Oxford University Press on behalf of the Annals of Botany Company. Background and Aims Many gymnosperms produce an ovular secretion, the pollination drop, during reproduction. The drops serve as a landing site for pollen, but also contain a suite of ions and organic compounds, including proteins, that suggests diverse roles for the drop during pollination. Proteins in the drops of species of Chamaecyparis, Juniperus, Taxus, Pseudotsuga, Ephedra and Welwitschia are thought to function in the conversion of sugars, defence against pathogens, and pollen growth and development. To better understand gymnosperm pollination biology, the pollination …

Destruction Of Opportunistic Pathogens Via Polymer Nanoparticle-Mediated Release Of Plant-Based Antimicrobial Payloads, Dahlia N. Amato, Douglas V. Amato, Olga V. Mavrodi, Dwaine A. Braasch, Susan E. Walley, Jessica R. Douglas, Dmitri V. Mavrodi, Derek L. Patton

Faculty Publications

The synthesis of antimicrobial thymol/carvacrol‐loaded polythioether nanoparticles (NPs) via a one‐pot, solvent‐free miniemulsion thiol‐ene photopolymerization process is reported. The active antimicrobial agents, thymol and carvacrol, are employed as “solvents” for the thiol‐ene monomer phase in the miniemulsion to enable facile high capacity loading (≈50% w/w), excellent encapsulation efficiencies (>95%), and elimination of all postpolymerization purification processes. The NPs serve as high capacity reservoirs for slow‐release and delivery of thymol/carvacrol‐combination payloads that exhibit inhibitory and bactericidal activity (>99.9% kill efficiency at 24 h) against gram‐positive and gram‐negative bacteria, including both saprophytic (Bacillus subtilis ATCC 6633 and Escherichia coli …

Replacing Arginine 33 For Alanine In The Hemophore Hasa From Pseudomonas Aeruginosa Causes Closure Of The H32 Loop In The Apo-Protein, Ritesh Kumar, Yifei Qi, Hirotoshi Matsumura, Scott Lovell, Huili Yao, Kevin P. Battaile, Wonpil Im, Pierre Moënne-Loccoz, Mario Rivera

Faculty Publications

© 2016 American Chemical Society. Previous characterization of hemophores from Serratia marcescens (HasAs), Pseudomonas aeruginosa (HasAp), and Yersinia pestis (HasAyp) showed that hemin binds between two loops, where it is axially coordinated by H32 and Y75. The Y75 loop is structurally conserved in all three hemophores and harbors conserved ligand Y75. The other loop contains H32 in HasAs and HasAp, but a noncoordinating Q32 in HasAyp. The H32 loop in apo-HasAs and apo-HasAp is in an open conformation, which places H32 about 30 Å from the hemin-binding site. Hence, hemin binding onto the Y75 loop of HasAs or HasAp triggers …

Perceptions And Experiences Of Human Papillomavirus (Hpv) Infection And Testing Among Low-Income Mexican Women, Leith León-Maldonado, Emily Wentzell, Brandon Brown, Betania Allen-Leigh, Leticia Torres-Ibarra, Jorge Salmerón, Deborah L. Billings, Jim Thrasher, Eduardo Lazcano-Ponce

Faculty Publications

Background

HPV infection causes cervical cancer, a major contributor to morbidity and mortality among low-income Mexican women. Human papillomavirus (HPV) DNA testing is now a primary screening strategy in Mexico’s early cervical cancer detection program (ECDP). Research on Mexican women’s perceptions of HPV and testing is necessary for establishing culturally appropriate protocols and educational materials. Here, we explore perceptions about HPV and HPV-related risk factors among low-income Mexican ECDP participants.

Methods

We conducted semi-structured interviews with 24 ECDP participants from two primary care health clinics in Michoacán state, Mexico. Interviews addressed women’s understandings of and experiences with HPV and HPV …

Biological Hot Spots And The Accumulation Of Marine Dissolved Organic Matter In A Highly Productive Ocean Margin, Yuan Shen, Cédric G. Fichot, Sheng-Kang Liang, Ronald Benner

Faculty Publications

Concentrations of dissolved organic carbon (DOC) and major biochemicals (amino acids and carbohydrates) were measured during five cruises (2009–2010) to the Louisiana margin in the northern Gulf of Mexico. Concentrations of amino acids and carbohydrates were elevated at mid-salinities and were indicative of plankton production of dissolved organic matter (DOM) in surface waters. Hot spots of two compositionally distinct types of labile DOM were identified based on the relative abundances of amino acids and carbohydrates. Amino acid-rich hot spots occurred sporadically in regions of high phytoplankton biomass and were mostly observed between dusk and dawn, reflecting a grazing source. In …

Comprehensive Analysis And Expression Profiling Of The Oslax And Osabcb Auxin Transporter Gene Families In Rice (Oryza Sativa) Under Phytohormone Stimuli And Abiotic Stresses, Chenglin Chai, Prasanta K. Subudhi

Faculty Publications

The plant hormone auxin regulates many aspects of plant growth and developmental processes. Auxin gradient is formed in plant as a result of polar auxin transportation by three types of auxin transporters such as OsLAX, OsPIN, and OsABCB. We report here the analysis of two rice auxin transporter gene families, OsLAX and OsABCB, using bioinformatics tools, publicly accessible microarray data, and quantitative RT-PCR. There are 5 putative OsLAXs and 22 putative OsABCBs in rice genome, which were mapped on 8 chromosomes. The exon-intron structure of OsLAX genes and properties of deduced proteins were relatively conserved within grass family, while that …

Wrinkled1 Rescues Feedback Inhibition Of Fatty Acid Synthesis In Hydroxylase-Expressing Seeds, Neil D. Adhikari, Philip D. Bates, John Browse

Faculty Publications

Previous attempts at engineering Arabidopsis (Arabidopsis thaliana) to produce seed oils containing hydroxy fatty acids (HFA) have resulted in low yields of HFA compared with the native castor (Ricinus communis) plant and caused undesirable effects, including reduced total oil content. Recent studies have led to an understanding of problems involved in the accumulation of HFA in oils of transgenic plants, which include metabolic bottlenecks and a decrease in the rate of fatty acid synthesis. Focusing on engineering the triacylglycerol assembly mechanisms led to modest increases in the HFA content of seed oil, but much room for …

Biological Aggregation Driven By Social And Environmental Factors: A Nonlocal Model And It Degenerate Cahn-Hilliard Approximation, Andrew J. Bernoff, Chad Topaz

Faculty Publications

No abstract provided.

Long-Term Shifts In The Patterns And Underlying Processes Of Plant Associations In Wisconsin Forests, Daijiang Li, Donald Waller

Faculty Publications

© 2016 John Wiley & Sons Ltd. Aim: Plant species co-occur within communities in response to variation in environmental conditions, limited species dispersal and biotic interactions. We used surveys and resurveys of the same sites of three temperate forest plant communities to study patterns of association between co-occurring species pairs and to infer how these mechanisms contribute to community assembly. Our goal was to compare these forces among communities occupying more and less disturbed landscapes and examine how these have changed over the last 50 years. Location: Wisconsin, USA. Methods: We resurveyed 266 sites first surveyed in the 1950s to …