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Effects Of Optical Turbulence And Density Gradients On Particle Image Velocimetry, Silvia Matt, Gero Nootz, Samuel Hellman, Weilin Hou Feb 2020

Effects Of Optical Turbulence And Density Gradients On Particle Image Velocimetry, Silvia Matt, Gero Nootz, Samuel Hellman, Weilin Hou

Faculty Publications

Particle image velocimetry (PIV) is a well-established tool to collect high-resolution velocity and turbulence data in the laboratory, in both air and water. Laboratory experiments are often performed under conditions of constant temperature or salinity or in flows with only small gradients of these properties. At larger temperature or salinity variations, the changes in the index of refraction of water or air due to turbulent microstructure can lead to so-called optical turbulence. We observed a marked influence of optical turbulence on particle imaging in PIV. The effect of index of refraction variations on PIV has been described in air for …

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De Novo Assembly Of A High-Quality Reference Genome For The Horned Lark (Eremophila Alpestris), Nicholas A. Mason, Paulo Pulgarin, Carlos Daniel Cadena, Irby J. Lovette Feb 2020

De Novo Assembly Of A High-Quality Reference Genome For The Horned Lark (Eremophila Alpestris), Nicholas A. Mason, Paulo Pulgarin, Carlos Daniel Cadena, Irby J. Lovette

Faculty Publications

Copyright © 2020 Mason et al. The Horned Lark (Eremophila alpestris) is a small songbird that exhibits remarkable geographic variation in appearance and habitat across an expansive distribution. While E. alpestris has been the focus of many ecological and evolutionary studies, we still lack a highly contiguous genome assembly for the Horned Lark and related taxa (Alaudidae). Here, we present CLO_EAlp_1.0, a highly contiguous assembly for E. alpestris generated from a blood sample of a wild, male bird captured in the Altiplano Cundiboyacense of Colombia. By combining short-insert and mate-pair libraries with the ALLPATHS-LG genome assembly pipeline, we generated a …

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Bromodomain And Extraterminal Inhibition By Jq1 Produces Divergent Transcriptional Regulation Of Suppressors Of Cytokine Signaling Genes In Adipocytes, Paula Mota De Sá, Allison J. Richard, Jacqueline M. Stephens Feb 2020

Bromodomain And Extraterminal Inhibition By Jq1 Produces Divergent Transcriptional Regulation Of Suppressors Of Cytokine Signaling Genes In Adipocytes, Paula Mota De Sá, Allison J. Richard, Jacqueline M. Stephens

Faculty Publications

© 2019 Endocrine Society 2019. All rights reserved. The Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway has cell-specific functions. Suppressors of cytokine signaling (SOCS) proteins are negative-feedback regulators of JAK-STAT signaling. STAT5 plays a significant role in adipocyte development and function, and bromodomain and extraterminal (BET) proteins may be involved in STAT5 transcriptional activity. We treated 3T3-L1 adipocytes with the BET inhibitor JQ1 and observed that growth hormone (GH)-induced expression of 2 STAT5 target genes from the SOCS family, Socs3 and Cish, were inversely regulated (increased and decreased, respectively) by BET inhibition. Chromatin immunoprecipitation analyses revealed that …

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Bromodomain And Extraterminal Inhibition By Jq1 Produces Divergent Transcriptional Regulation Of Suppressors Of Cytokine Signaling Genes In Adipocytes, Paula Mota De Sá, Allison J. Richard, Jacqueline M. Stephens Feb 2020

Bromodomain And Extraterminal Inhibition By Jq1 Produces Divergent Transcriptional Regulation Of Suppressors Of Cytokine Signaling Genes In Adipocytes, Paula Mota De Sá, Allison J. Richard, Jacqueline M. Stephens

Faculty Publications

© Endocrine Society 2019. All rights reserved. For permissions, please e-mail: [email protected]. The Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway has cell-specific functions. Suppressors of cytokine signaling (SOCS) proteins are negative-feedback regulators of JAK-STAT signaling. STAT5 plays a significant role in adipocyte development and function, and bromodomain and extraterminal (BET) proteins may be involved in STAT5 transcriptional activity. We treated 3T3-L1 adipocytes with the BET inhibitor JQ1 and observed that growth hormone (GH)-induced expression of 2 STAT5 target genes from the SOCS family, Socs3 and Cish, were inversely regulated (increased and decreased, respectively) by BET inhibition. Chromatin …

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Mitochondrial Pyruvate Carriers Are Not Required For Adipogenesis But Are Regulated By High-Fat Feeding In Brown Adipose Tissue, Jasmine A. Burrell, Allison J. Richard, William T. King, Jacqueline M. Stephens Feb 2020

Mitochondrial Pyruvate Carriers Are Not Required For Adipogenesis But Are Regulated By High-Fat Feeding In Brown Adipose Tissue, Jasmine A. Burrell, Allison J. Richard, William T. King, Jacqueline M. Stephens

Faculty Publications

© 2020 The Obesity Society. Objective: The objectives of this study were to assess the role of mitochondrial pyruvate carriers (MPCs) in adipocyte development in vitro and determine whether MPCs are regulated in vivo by high-fat feeding in male and female C57BL/6J mice. Methods: This study utilized small interfering RNA-mediated knockdown to assess the requirement of MPC1 for adipogenesis in the 3T3-L1 model system. Treatment with UK-5099, a potent pharmacological MPC inhibitor, was also used to assess the loss of MPC activity. Western blot analysis was performed on adipose tissue samples from mice on a low-fat diet or a high-fat …

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Ncapg Dynamically Coordinates The Myogenesis Of Fetal Bovine Tissue By Adjusting Chromatin Accessibility, Xin Hu, Yishen Xing, Xing Fu, Qiyuan Yang, Ling Ren, Yahui Wang, Qian Li, Junya Li, Lupei Zhang Feb 2020

Ncapg Dynamically Coordinates The Myogenesis Of Fetal Bovine Tissue By Adjusting Chromatin Accessibility, Xin Hu, Yishen Xing, Xing Fu, Qiyuan Yang, Ling Ren, Yahui Wang, Qian Li, Junya Li, Lupei Zhang

Faculty Publications

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. NCAPG is a subunit of condensin I that plays a crucial role in chromatin condensation during mitosis. NCAPG has been demonstrated to be associated with farm animal growth traits. However, its role in regulating myoblast differentiation is still unclear. We used myoblasts derived from fetal bovine tissue as an in vitro model and found that NCAPG was expressed during myogenic differentiation in the cytoplasm and nucleus. Silencing NCAPG prolonged the mitosis and impaired the differentiation due to increased myoblast apoptosis. After 1.5 days of differentiation, silencing NCAPG enhanced muscle-specific gene expression. …

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Perinatal Maternal Antibiotic Exposure Augments Lung Injury In Offspring In Experimental Bronchopulmonary Dysplasia, Kent A. Willis, David T. Siefker, Michael M. Aziz, Catrina T. White, Naiha Mussarat, Charles K. Gomes, Amandeep Bajwa, Joseph F. Pierre, Stephania A. Cormier, Ajay J. Talati Feb 2020

Perinatal Maternal Antibiotic Exposure Augments Lung Injury In Offspring In Experimental Bronchopulmonary Dysplasia, Kent A. Willis, David T. Siefker, Michael M. Aziz, Catrina T. White, Naiha Mussarat, Charles K. Gomes, Amandeep Bajwa, Joseph F. Pierre, Stephania A. Cormier, Ajay J. Talati

Faculty Publications

Copyright © 2020 the American Physiological Society. During the newborn period, intestinal commensal bacteria influence pulmonary mucosal immunology via the gut-lung axis. Epidemiological studies have linked perinatal antibiotic exposure in human newborns to an increased risk for bronchopulmonary dysplasia, but whether this effect is mediated by the gut-lung axis is unknown. To explore antibiotic disruption of the newborn gut-lung axis, we studied how perinatal maternal antibiotic exposure influenced lung injury in a hyperoxia-based mouse model of bronchopulmonary dysplasia. We report that disruption of intestinal commensal colonization during the perinatal period promotes a more severe bronchopulmonary dysplasia phenotype characterized by increased …

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Mice Without A Microbiome Are Partially Protected From Lung Injury By Hyperoxia, Kent A. Willis, Joseph F. Pierre, Stephania A. Cormier, Ajay J. Talati Feb 2020

Mice Without A Microbiome Are Partially Protected From Lung Injury By Hyperoxia, Kent A. Willis, Joseph F. Pierre, Stephania A. Cormier, Ajay J. Talati

Faculty Publications

No abstract provided.

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Identification Of A Pai-1-Binding Site Within An Intrinsically Disordered Region Of Vitronectin, Yuzhuo Chu, Joel C. Bucci, Cynthia B. Peterson Feb 2020

Identification Of A Pai-1-Binding Site Within An Intrinsically Disordered Region Of Vitronectin, Yuzhuo Chu, Joel C. Bucci, Cynthia B. Peterson

Faculty Publications

© 2019 The Protein Society The serine protease inhibitor, plasminogen activator inhibitor Type-1 (PAI-1) is a metastable protein that undergoes an unusual transition to an inactive conformation with a short half-life of only 1–2 hr. Circulating PAI-1 is bound to a cofactor vitronectin, which stabilizes PAI-1 by slowing this latency conversion. A well-characterized PAI-1-binding site on vitronectin is located within the somatomedin B (SMB) domain, corresponding to the first 44 residues of the protein. Another PAI-1 recognition site has been identified with an engineered form of vitronectin lacking the SMB domain, yet retaining PAI-1 binding capacity (Schar, Blouse, Minor, Peterson. …

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Mud Deposition And Diagenesis Within An Early Palaeozoic Clinothem: Power Steps Formation, Newfoundland, Canada, Kathryn C. Denommee, Dario Harazim, Samuel J. Bentley, James H. Macquaker, Stefanie Lode, Babatunde John Olanipekun Feb 2020

Mud Deposition And Diagenesis Within An Early Palaeozoic Clinothem: Power Steps Formation, Newfoundland, Canada, Kathryn C. Denommee, Dario Harazim, Samuel J. Bentley, James H. Macquaker, Stefanie Lode, Babatunde John Olanipekun

Faculty Publications

© Cambridge University Press 2019. The early Ordovician (∼385 Ma) Power Steps Formation, Newfoundland, Canada, exposes a well-preserved mudstone-dominated clinothem that serves as an excellent archive for understanding how mud has been produced, transported and converted into mudstone prior to the evolution of globally widespread, deep soil horizons. Sedimentological analysis of four sandstone and five mudstone facies, along the Ochre Cove clinothem, reveal that mud and sand were delivered by unidirectional currents and experienced episodic reworking by storm waves. Petrographic examination and X-ray diffraction from described mudstone facies reveal significant variability in the distribution of illite versus chlorite between the …

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Erratum: The Extended Specimen Network: A Strategy To Enhance Us Biodiversity Collections, Promote Research And Education (Bioscience Doi: 10.1093/Biosci/Biz140, James Lendemer, Barbara Thiers, Anna K. Monfils, Jennifer Zaspel, Elizabeth R. Ellwood, Andrew Bentley, Katherine Levan, John Bates, David Jennings, Dori Contreras, Laura Lagomarsino, Paula Mabee, Linda S. Ford, Robert Guralnick, Robert E. Gropp, Marcy Revelez, Neil Cobb, Katja Seltmann, M. Catherine Aime Feb 2020

Erratum: The Extended Specimen Network: A Strategy To Enhance Us Biodiversity Collections, Promote Research And Education (Bioscience Doi: 10.1093/Biosci/Biz140, James Lendemer, Barbara Thiers, Anna K. Monfils, Jennifer Zaspel, Elizabeth R. Ellwood, Andrew Bentley, Katherine Levan, John Bates, David Jennings, Dori Contreras, Laura Lagomarsino, Paula Mabee, Linda S. Ford, Robert Guralnick, Robert E. Gropp, Marcy Revelez, Neil Cobb, Katja Seltmann, M. Catherine Aime

Faculty Publications

© 2020 The Author(s). In a recent article (doi:10.1093/biosci/biz140), in the original publication of this report, Marcy Revelez's affiliation was listed as the Centers for Disease Control and Prevention, Atlanta, GA 30333. It has since been changed to correctly reflect affiliation with Museum of Texas Tech University, Lubbock, TX 79409-3191.

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Respiratory Syncytial Virus Disease Severity Is Associated With Distinct Cd8+ T-Cell Profiles, David T. Siefker, Luan Vu, Dahui You, Andrew Mcbride, Ryleigh Taylor, Tamekia L. Jones, John Devincenzo, Stephania A. Cormier Feb 2020

Respiratory Syncytial Virus Disease Severity Is Associated With Distinct Cd8+ T-Cell Profiles, David T. Siefker, Luan Vu, Dahui You, Andrew Mcbride, Ryleigh Taylor, Tamekia L. Jones, John Devincenzo, Stephania A. Cormier

Faculty Publications

Copyright © 2020 by the American Thoracic Society. Rationale: Respiratory syncytial virus (RSV) causes significant morbidity and mortality in infants worldwide. Although T-helper type 2 (Th2) cell pathology is implicated in severe disease, the mechanisms underlying the development of immunopathology are incompletely understood. Objectives: We aimed to identify local immune responses associated with severe RSV in infants. Our hypothesis was that disease severity would correlate with enhanced Th2 cellular responses. Methods: Nasal aspirates were collected from infants hospitalized with severe (admitted to the pediatric ICU) or moderate (maintained in the general ward) RSV disease at 5 to 9 days after …

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Hot-Water Baths, Biologicals And Re-Curing Effects On Rhizopus Soft Rot During Sweetpotato Packing, R. R. Sweany, D. H. Picha, C. A. Clark Feb 2020

Hot-Water Baths, Biologicals And Re-Curing Effects On Rhizopus Soft Rot During Sweetpotato Packing, R. R. Sweany, D. H. Picha, C. A. Clark

Faculty Publications

Rhizopus soft rot (RSR) caused by Rhizopus stolonifer is one of the most devastating postharvest diseases of sweetpotato. It causes greatest losses when sweetpotatoes are removed from storage, washed and packed for marketing. The disease has been managed effectively by prophylactic application of synthetic fungicides on the packing line. However, there is increasing demand for alternative management strategies that do not rely on prophylactic use of synthetic fungicides. While curing immediately after harvest is a standard industry practice, re-curing after storage is not widely practised for postharvest disease management. In this study, the use of hot-water baths, biocontrol agents and …

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Synthesis, Characterization, Dna Binding, Topoisomerase Inhibition, And Apoptosis Induction Studies Of A Novel Cobalt(Iii) Complex With A Thiosemicarbazone Ligand, Stephen J. Beebe, Michael J. Celestine, Jimmie L. Bullock, Shayna Sandhaus, Jessa Faye Arca, Donald M. Cropek, Tekettay A. Ludvig, Sydney R. Foster, Jasmine S. Clark, Floyd A. Beckford, Criszcele M. Tano, Elizabeth A. Tonsel-White, Raj K. Gurung, Courtney E. Stankavich, Yuk Ching Tse-Dinh, William L. Jarrett, Alvin A. Holder Feb 2020

Synthesis, Characterization, Dna Binding, Topoisomerase Inhibition, And Apoptosis Induction Studies Of A Novel Cobalt(Iii) Complex With A Thiosemicarbazone Ligand, Stephen J. Beebe, Michael J. Celestine, Jimmie L. Bullock, Shayna Sandhaus, Jessa Faye Arca, Donald M. Cropek, Tekettay A. Ludvig, Sydney R. Foster, Jasmine S. Clark, Floyd A. Beckford, Criszcele M. Tano, Elizabeth A. Tonsel-White, Raj K. Gurung, Courtney E. Stankavich, Yuk Ching Tse-Dinh, William L. Jarrett, Alvin A. Holder

Faculty Publications

In this study, 9-anthraldehyde-N(4)-methylthiosemicarbazone (MeATSC) 1 and [Co(phen)2(O2CO)]Cl·6H2O 2 (where phen = 1,10-phenanthroline) were synthesized. [Co(phen)2(O2CO)]Cl·6H2O 2 was used to produce anhydrous [Co(phen)2(H2O)2](NO3)3 3. Subsequently, anhydrous [Co(phen)2(H2O)2](NO3)3 3 was reacted with MeATSC 1 to produce [Co(phen)2(MeATSC)](NO3)3·1.5H2O·C2H5OH 4. The ligand, MeATSC 1 and all complexes were characterized by elemental analysis, FT IR, UV–visible, and multinuclear NMR ( …

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Experimental Forest Fragmentation Alters Amazonian Mixed-Species Flocks, Cameron L. Rutt, Karl Mokross, Michael D. Kaller, Philip C. Stouffer Feb 2020

Experimental Forest Fragmentation Alters Amazonian Mixed-Species Flocks, Cameron L. Rutt, Karl Mokross, Michael D. Kaller, Philip C. Stouffer

Faculty Publications

Habitat fragmentation has been associated with myriad negative effects for forest-dependent birds in the Neotropics. However, the vast majority of negative effects have been inferred from comparisons of pre-existing fragments with separate control sites. Such comparisons confound area loss with isolation and ignore effects of patchy distributions and local habitat heterogeneity. To directly test the effects of fragmentation on Amazonian mixed-species flocks—a complex and diverse species interaction network—we observed birds before and after re-isolation of three 10-ha fragments at the Biological Dynamics of Forest Fragments Project in central Amazonia. Following initial isolation in the 1980s, these fragments have been surrounded …

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Rubusoside-Assisted Solubilization Of Poorly Soluble C6-Ceramide For A Pilot Pharmacokinetic Study, Jianzhong Chen, Sachin K. Khiste, Xiaomei Fu, Kartik R. Roy, Yixuan Dong, Jian Zhang, Mei Liu, Yong Yu Liu, Zhijun Liu Feb 2020

Rubusoside-Assisted Solubilization Of Poorly Soluble C6-Ceramide For A Pilot Pharmacokinetic Study, Jianzhong Chen, Sachin K. Khiste, Xiaomei Fu, Kartik R. Roy, Yixuan Dong, Jian Zhang, Mei Liu, Yong Yu Liu, Zhijun Liu

Faculty Publications

Although C6-Ceramide has attracted much attention as a possible tumor suppressor, the delivery of C6-Ceramide is still challenging due to its inherent hydrophobicity and insolubility. In this study we explored the use of a natural compound rubusoside (RUB) as a solubilizer to enhance the solubility of a fluorescence-labeled C6-Ceramide (NBD C6-Ceramide) and to characterize its pharmacokinetics and tissue distribution in an animal model. RUB significantly enhanced the solubility of NBD C6-Ceramide by forming nanomicelles, and efficiently delivered NBD C6-Ceramide in rats by oral and intravenous administration. RUB loaded 1.96 % of NBD C6-Ceramide in the nanomicelles and solubilized it to …

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The Response Of Stomatal Conductance To Seasonal Drought In Tropical Forests, Jin Wu, Shawn P. Serbin, Kim S. Ely, Brett T. Wolfe, L. Turin Dickman, Charlotte Grossiord, Sean T. Michaletz, Adam D. Collins, Matteo Detto, Nate G. Mcdowell, S. Joseph Wright, Alistair Rogers Feb 2020

The Response Of Stomatal Conductance To Seasonal Drought In Tropical Forests, Jin Wu, Shawn P. Serbin, Kim S. Ely, Brett T. Wolfe, L. Turin Dickman, Charlotte Grossiord, Sean T. Michaletz, Adam D. Collins, Matteo Detto, Nate G. Mcdowell, S. Joseph Wright, Alistair Rogers

Faculty Publications

Stomata regulate CO2 uptake for photosynthesis and water loss through transpiration. The approaches used to represent stomatal conductance (gs) in models vary. In particular, current understanding of drivers of the variation in a key parameter in those models, the slope parameter (i.e. a measure of intrinsic plant water-use-efficiency), is still limited, particularly in the tropics. Here we collected diurnal measurements of leaf gas exchange and leaf water potential (Ψleaf), and a suite of plant traits from the upper canopy of 15 tropical trees in two contrasting Panamanian forests throughout the dry season of the 2016 El Niño. The plant traits …

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Trans-Disciplinary Responses To Climate Change: Lessons From Rice-Based Systems In Asia, Jon Hellin, Jean Balié, Eleanor Fisher, Ajay Kohli, Melanie Connor, Sudhir Yadav, Virender Kumar, Timothy J. Krupnik, Bjoern Ole Sander, Joshua Cobb, Katherine Nelson, Tri Setiyono, Ranjitha Puskur, Pauline Chivenge, Martin Gummert Feb 2020

Trans-Disciplinary Responses To Climate Change: Lessons From Rice-Based Systems In Asia, Jon Hellin, Jean Balié, Eleanor Fisher, Ajay Kohli, Melanie Connor, Sudhir Yadav, Virender Kumar, Timothy J. Krupnik, Bjoern Ole Sander, Joshua Cobb, Katherine Nelson, Tri Setiyono, Ranjitha Puskur, Pauline Chivenge, Martin Gummert

Faculty Publications

Climate change will continue to have a largely detrimental impact on the agricultural sector worldwide because of predicted rising temperatures, variable rainfall, and an increase in extreme weather events. Reduced crop yields will lead to higher food prices and increased hardship for low income populations, especially in urban areas. Action on climate change is one of the Sustainable Development Goals (SDG 13) and is linked to the Paris Climate Agreement. The research challenge posed by climate change is so complex that a trans-disciplinary response is required, one that brings together researchers, practitioners, and policy-makers in networks where the lines between …

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Evaluation Of Ultraviolet (Uv-C) Light Treatment For Microbial Inactivation In Agricultural Waters With Different Levels Of Turbidity, Achyut Adhikari, Katheryn J. Parraga Estrada, Vijay S. Chhetri, Marlene Janes, Kathryn Fontenot, John C. Beaulieu Feb 2020

Evaluation Of Ultraviolet (Uv-C) Light Treatment For Microbial Inactivation In Agricultural Waters With Different Levels Of Turbidity, Achyut Adhikari, Katheryn J. Parraga Estrada, Vijay S. Chhetri, Marlene Janes, Kathryn Fontenot, John C. Beaulieu

Faculty Publications

Produce growers using surface or well water to irrigate their crops may require an appropriate water treatment system in place to meet the water quality standard imposed by FSMA Produce Safety Rule. This study evaluated the potential of using ultraviolet (UV-C) treatment in reducing the microbial population in agricultural water. Waters with turbidity levels ranging from 10.93 to 23.32 Nephelometric Turbidity Units (NTU) were prepared by mixing pond water and well water. The waters were inoculated with a cocktail of generic Escherichia coli (ATCC 23716, 25922, and 11775) and then treated with UV-C light (20–60 mJ/cm2). All tested doses of …

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Plumage Patterns: Ecological Functions, Evolutionary Origins, And Advances In Quantification, Nicholas A. Mason, Rauri C.K. Bowie Feb 2020

Plumage Patterns: Ecological Functions, Evolutionary Origins, And Advances In Quantification, Nicholas A. Mason, Rauri C.K. Bowie

Faculty Publications

Birds exhibit remarkable variation in plumage patterns, both within individual feathers and among plumage patches. Differences in the size, shape, and location of pigments and structural colors comprise important visual signals involved in mate choice, social signaling, camouflage, and many other functions. While ornithologists have studied plumage patterns for centuries, recent technological advances in digital image acquisition and processing have transformed pattern quantification methods, enabling comprehensive, detailed datasets of pattern phenotypes that were heretofore inaccessible. In this review, we synthesize recent and classic studies of plumage patterns at different evolutionary and organismal scales and discuss the various roles that plumage …

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Fine Structure Of Viral Dsdna Encapsidation, Shawn Walker, Javier Arsuaga, Lindsey Hiltner, M. Carme Calderer, Mariel Vázquez Feb 2020

Fine Structure Of Viral Dsdna Encapsidation, Shawn Walker, Javier Arsuaga, Lindsey Hiltner, M. Carme Calderer, Mariel Vázquez

Faculty Publications

Unraveling the mechanisms of packing of DNA inside viral capsids is of fundamental importance to understanding the spread of viruses. It could also help develop new applications to targeted drug delivery devices for a large range of therapies. In this article, we present a robust, predictive mathematical model and its numerical implementation to aid the study and design of bacteriophage viruses for application purposes. Exploiting the analogies between the columnar hexagonal chromonic phases of encapsidated viral DNA and chromonic aggregates formed by plank-shaped molecular compounds, we develop a first-principles effective mechanical model of DNA packing in a viral capsid. The …

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Agricultural And Finance Intervention Increased Dietary Intake And Weight Of Children Living In Hiv-Affected Households In Western Kenya, Lisa M. Butler, Shiva Bhandari, Phelgona Otieno, Sheri D. Weiser, Craig R. Cohen, Edward A. Frongillo Jr. Feb 2020

Agricultural And Finance Intervention Increased Dietary Intake And Weight Of Children Living In Hiv-Affected Households In Western Kenya, Lisa M. Butler, Shiva Bhandari, Phelgona Otieno, Sheri D. Weiser, Craig R. Cohen, Edward A. Frongillo Jr.

Faculty Publications

We tested whether a multisectoral household agricultural and finance intervention increased the dietary intake and improved the nutritional status of HIV-affected children. Two hospitals in rural Kenya were randomly assigned to be either the intervention or the control arm. The intervention comprised a human-powered water pump, microfinance loan for farm commodities, and training in sustainable farming practices and financial management. In each arm, 100 children (0-59 mo of age) were enrolled from households with HIV-infected adults 18-49 y old. Children were assessed beginning in April 2012 and every 3 mo for 1 y for dietary intake and anthropometry. Children in …

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Energetic And Environmental Constraints On The Community Structure Of Benthic Microbial Mats In Lake Fryxell, Antarctica, Megan L. Dillon, Ian Hawes, Anne D. Jungblut, Tyler J. Mackey, Jonathan A. Eisen, Peter T. Doran, Dawn Y. Sumner Feb 2020

Energetic And Environmental Constraints On The Community Structure Of Benthic Microbial Mats In Lake Fryxell, Antarctica, Megan L. Dillon, Ian Hawes, Anne D. Jungblut, Tyler J. Mackey, Jonathan A. Eisen, Peter T. Doran, Dawn Y. Sumner

Faculty Publications

© 2020 FEMS 2020. Ecological communities are regulated by the flow of energy through environments. Energy flow is typically limited by access to photosynthetically active radiation (PAR) and oxygen concentration (O2). The microbial mats growing on the bottom of Lake Fryxell, Antarctica, have well-defined environmental gradients in PAR and (O2). We analyzed the metagenomes of layers from these microbial mats to test the extent to which access to oxygen and light controls community structure. We found variation in the diversity and relative abundances of Archaea, Bacteria and Eukaryotes across three (O2) and PAR conditions: high (O2) and maximum PAR, variable …

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Emerging Decolonialized Research Collaboration: The Max Planck Society And The Leibniz Association In Latin America, Pedro Pineda, Gustavo Gregorutti, Bernhard Streitwieser Feb 2020

Emerging Decolonialized Research Collaboration: The Max Planck Society And The Leibniz Association In Latin America, Pedro Pineda, Gustavo Gregorutti, Bernhard Streitwieser

Faculty Publications

Analyzing the number of publications and proportion of corresponding authors of Latin American scholars and scholars from the German Max Planck Society (MPS) and the Leibniz Association (LA; 1954–2018), this article asks if North–South partnerships continue to represent power imbalances. Our bibliometric analysis indicates that (a) in comparison with the LA, the MPS’s scientists published more articles with Latin American countries, led by Brazil, Chile, Argentina, and Mexico; (b) researchers from the MPS and the LA frequently took the role of corresponding author; (c) researchers from Brazil, Chile, Argentina, and Mexico primarily controlled their region’s productivity, but (d) Brazil built …

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Highly Active Cationic Cobalt(Ii) Hydroformylation Catalysts, Drew M. Hood, Ryan A. Johnson, Alex E. Carpenter, Jarod M. Younker, David J. Vinyard, George G. Stanley Jan 2020

Highly Active Cationic Cobalt(Ii) Hydroformylation Catalysts, Drew M. Hood, Ryan A. Johnson, Alex E. Carpenter, Jarod M. Younker, David J. Vinyard, George G. Stanley

Faculty Publications

© 2020 American Association for the Advancement of Science. All rights reserved. The cobalt complexes HCo(CO)4 and HCo(CO)3(PR3) were the original industrial catalysts used for the hydroformylation of alkenes through reaction with hydrogen and carbon monoxide to produce aldehydes. More recent and expensive rhodium-phosphine catalysts are hundreds of times more active and operate under considerably lower pressures. Cationic cobalt(II) bisphosphine hydrido-carbonyl catalysts that are far more active than traditional neutral cobalt(I) catalysts and approach rhodium catalysts in activity are reported here. These catalysts have low linear-to-branched (L:B) regioselectivity for simple linear alkenes. However, owing to their high alkene isomerization activity …

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Recombination And Gene Loss Occur Simultaneously During Bacterial Horizontal Gene Transfer, Bert Ely Jan 2020

Recombination And Gene Loss Occur Simultaneously During Bacterial Horizontal Gene Transfer, Bert Ely

Faculty Publications

Bacteria can acquire new genes by incorporating environmental DNA into their genomes, yet genome sizes stay relatively constant. In nature, gene acquisition is a rare event so it is difficult to observe. However, the Caulobacter crescentus CB2A genome contains 114 insertions of genetic material from the closely-related NA1000 strain, providing a unique opportunity to analyze the horizontal transfer of genetic material. Analyses of these insertions led to a new model that involves preferential recombination at non-homologous regions that are flanked by regions of homology and does not involve any mutational processes. The net result is the replacement of segments of …

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Functional And Transcriptomic Characterization Of Cisplatin-Resistant Ags And Mkn-28 Gastric Cancer Cell Lines, Barbara Mora-Lagos, Irene Cartas-Espinel, Ismael Riquelme, Alyssa C. Parker, Stephen R. Piccolo, Tamara Viscarra, Maria Elena Reyes, Louise Zanella, Kurt Buchegger, Carmen Ili, Priscilla Brebi Jan 2020

Functional And Transcriptomic Characterization Of Cisplatin-Resistant Ags And Mkn-28 Gastric Cancer Cell Lines, Barbara Mora-Lagos, Irene Cartas-Espinel, Ismael Riquelme, Alyssa C. Parker, Stephen R. Piccolo, Tamara Viscarra, Maria Elena Reyes, Louise Zanella, Kurt Buchegger, Carmen Ili, Priscilla Brebi

Faculty Publications

Gastric cancer (GC) is a significant cancer-related cause of death worldwide. The most used chemotherapeutic regimen in GC is based on platinum drugs such as cisplatin (CDDP). However, CDDP resistance reduces advanced GC survival. In vitro drug-resistant cell model would help in the understanding of molecular mechanisms underlying this drug-resistance phenomenon. The aim of this study was to characterize new models of CDDP-resistant GC cell lines (AGS R-CDDP and MKN-28 R-CDDP) obtained through a stepwise increasing drug doses method, in order to understand the molecular mechanisms underlying chemoresistance as well as identify new therapeutic targets for the treatment of GC. …

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Green Edge Ice Camp Campaigns: Understanding The Processes Controlling The Under-Ice Arctic Phytoplankton Spring Bloom, Philippe Massicotte, Rémi Amiraux, Marie-Pier Amyot, Philippe Archambault, Mathieu Ardyna, Laurent Arnaud, Lise Artigue, Cyril Aubry, Pierre Ayotte, Guislain Bécu, Simon Bélanger, Ronald Benner, Henry C. Bittig, Annick Bricaud, Éric Brossier, Flavienne Bruyant, Et. Al. Jan 2020

Green Edge Ice Camp Campaigns: Understanding The Processes Controlling The Under-Ice Arctic Phytoplankton Spring Bloom, Philippe Massicotte, Rémi Amiraux, Marie-Pier Amyot, Philippe Archambault, Mathieu Ardyna, Laurent Arnaud, Lise Artigue, Cyril Aubry, Pierre Ayotte, Guislain Bécu, Simon Bélanger, Ronald Benner, Henry C. Bittig, Annick Bricaud, Éric Brossier, Flavienne Bruyant, Et. Al.

Faculty Publications

The Green Edge initiative was developed to investigate the processes controlling the primary productivity and fate of organic matter produced during the Arctic phytoplankton spring bloom (PSB) and to determine its role in the ecosystem. Two field campaigns were conducted in 2015 and 2016 at an ice camp located on landfast sea ice southeast of Qikiqtarjuaq Island in Baffin Bay (67.4797∘ N, 63.7895∘ W). During both expeditions, a large suite of physical, chemical and biological variables was measured beneath a consolidated sea-ice cover from the surface to the bottom (at 360 m depth) to better understand the factors driving the …

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A Transcriptional Regulatory System Of The S. Cerevisiae Ole1 Gene Responds To Fatty Acid Species And Intracellular Amount, And Not Simply Membrane Status, Marshall Willey, Matthew Ochs, Clara Schriemer, Virginia Mcdonough Jan 2020

A Transcriptional Regulatory System Of The S. Cerevisiae Ole1 Gene Responds To Fatty Acid Species And Intracellular Amount, And Not Simply Membrane Status, Marshall Willey, Matthew Ochs, Clara Schriemer, Virginia Mcdonough

Faculty Publications

We examined the effects of unsaturated fatty acid (UFA) species and their concentration on the expression of OLE1, which encodes the stearoyl CoA desaturase, in Saccharomyces cerevisiae. We controlled the amount of UFA taken up by the cell by varying the concentration of tergitol in the medium. When cultured with 1 mM fatty acid in 0.1% tergitol, cells took up much more fatty acid than when cultured with the same concentration of fatty acid at 1% tergitol, although the amount incorporated was dependent on UFA species. For each fatty acid tested, we found that the higher uptake (0.1% tergitol condition) …

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Production Of The Polysaccharide Curdlan By Agrobacterium Species On Processing Coproducts And Plant Lignocellulosic Hydrolysates, Thomas P. West Jan 2020

Production Of The Polysaccharide Curdlan By Agrobacterium Species On Processing Coproducts And Plant Lignocellulosic Hydrolysates, Thomas P. West

Faculty Publications

This review examines the production of the biopolymer curdlan, synthesized by Agrobacterium species(sp.),on processing coproducts and plant ligno cellulosichydrolysates. Curdlanis a-(1 3)-D-glucan that has various food, non-food and biomedical applications. A number of carbon sources support bacterial curdlan production upon depletion of nitrogen in the culture medium. The influence of culture medium pH is critical to the synthesis of curdlan. The biosynthesis of the-(1 3)-D-glucan is likely controlled by a regulatory protein that controls the genes involved in the bacterial production of curdlan. Curdlan overproducer mutant strains have been isolated from Agrobacterium sp. ATCC 31749 and ATCC 31750 by chemical …

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