Digital Commons Network^™

Dietary Quality And Dietary Inflammatory Potential During Pregnancy And Offspring Emotional And Behavioral Symptoms In Childhood: An Individual Participant Data Meta-Analysis Of Four European Cohorts, Kinga Polanska, Pawel Kaluzny, Adrien M. Aubert, Liesbeth Duijts, Hanan El Marroun, Wojciech Hanke, James R. Hébert, Barbara Heude, Agnieszka Jankowska, Giulia Mancano, Sara M. Mensink-Bout, Caroline Relton, Nitin Shivappa Mbbs, Mph, Ph.D., Matthew Suderman, Elzbieta Trafalska, Ewelina Wesolowska, Raquel Garcia-Esteban, Mònica Guxens, Maribel Casas, Catherine M. Phillips

Faculty Publications

Background

The impact of maternal diet during pregnancy on child neurodevelopment is of public health and clinical relevance. We evaluated the associations of dietary quality based on the Dietary Approaches to Stop Hypertension (DASH) score and dietary inflammatory potential based on the energy-adjusted Dietary Inflammatory Index (E-DII) score during pregnancy with emotional and behavioral symptoms of offspring at 7 to 10 years of age.

Methods

Individual participant data for 11,870 mother–child pairs from four European cohorts participating in the ALPHABET project were analyzed. Maternal antenatal DASH and E-DII scores were generated from self-completed food frequency questionnaires. Symptoms of depression …

Compoundhetvip: Compound Heterozygous Variant Identification Pipeline, Dustin B. Miller, Stephen R. Picollo

Faculty Publications

Compound Heterozygous ( CH) variant identification requires distinguishing maternally from paternally derived nucleotides, a process that requires numerous computational tools. Using such tools often introduces unforeseen challenges such as installation procedures that are operating-system specific, software dependencies that must be installed, and formatting requirements for input files. To overcome these challenges, we developed Compound Heterozygous Variant Identification Pipeline (CompoundHetVIP), which uses a single Docker image to encapsulate commonly used software tools for file aggregation ( BCFtools or GATK4), VCF liftover ( Picard Tools), joint-genotyping ( GATK4), file conversion ( Plink2), phasing ( SHAPEIT2, Beagle …

The Roles Of Identity Formation And Moral Identity In College Student Mental Health, Health-Risk Behaviors, And Psychological Well-Being, Sam A. Hardy, Stephen W. Francis, Byron L. Zamboanga, Su Yeong Kim, Spencer G. Anderson, Larry F. Forthun

Faculty Publications

Objectives: This study examined the roles of identity formation and moral identity in predicting college student mental health (anxiety and depressive symptoms), health-risk behaviors (hazardous alcohol use and sexual risk taking), and psychological well-being (self-esteem and meaning).

Method: The sample comprised 9,500 college students (aged 18–25 years, mean = 19.78, standard deviation = 1.61: 73% female; 62% European American), from 31 different universities, who completed an online self-report survey. Results: Structural equation models found that identity maturity (commitment making and identity synthesis) predicted 5 of the health outcomes (except sexual risk taking), and moral identity predicted ail of the health …

Mosquito Vector Production Across Socio-Economic Divides In Baton Rouge, Louisiana, Rebeca De Jesús Crespo, Madison Harrison, Rachel Rogers, Randy Vaeth

Faculty Publications

No abstract provided.

A Cluster-Based Approach For Identifying Prognostic Microrna Signatures In Digestive System Cancers, Jun Zhou, Xiang Cui, Feifei Xiao, Guoshuai Cai

Faculty Publications

Cancer remains the second leading cause of death all over the world. Aberrant expression of miRNA has shown diagnostic and prognostic value in many kinds of cancer. This study aims to provide a novel strategy to identify reliable miRNA signatures and develop improved cancer prognostic models from reported cancer-associated miRNAs. We proposed a new cluster-based approach to identify distinct cluster(s) of cancers and corresponding miRNAs. Further, with samples from TCGA and other independent studies, we identified prognostic markers and validated their prognostic value in prediction models. We also performed KEGG pathway analysis to investigate the functions of miRNAs associated with …

Assessing Chlorophyll A Spatiotemporal Patterns Combining In Situ Continuous Fluorometry Measurements And Landsat 8/Oli Data Across The Barataria Basin (Louisiana, Usa), Ivan A. Vargas-Lopez, Victor H. Rivera-Monroy, John W. Day, Julie Whitbeck, Kanchan Maiti, Christopher J. Madden, Armando Trasviña-Castro

Faculty Publications

The acquisition of reliable and accurate data to assess environmental changes over large spatial scales is one of the main limitations to determine the impact of eutrophication, and the effectiveness of management strategies in coastal systems. Here, we used a continuous in situ Chl-a fluorometry sensor and L8/OLI satellite data to develop an algorithm and map Chl-a spatial distribution to assess the impact of freshwater diversions and associated high nutrient loading rates in the Barataria Basin (BB) complex, a coastal system in the northern Gulf of Mexico. We collected water quality samples at 24 sampling stations and high-frequency continuous fluorometry …

Temporal And Spatial Blood Feeding Patterns Of Urban Mosquitoes In The San Juan Metropolitan Area, Puerto Rico, Matthew W. Hopken, Limarie J. Reyes-Torres, Nicole Scavo, Antoinette J. Piaggio, Zaid Abdo, Daniel Taylor, James Pierce, Donald A. Yee

Faculty Publications

Urban ecosystems are a patchwork of habitats that host a broad diversity of animal species. Insects comprise a large portion of urban biodiversity which includes many pest species, including those that transmit pathogens. Mosquitoes (Diptera: Culicidae) inhabit urban environments and rely on sympatric vertebrate species to complete their life cycles, and in this process transmit pathogens to animals and humans. Given that mosquitoes feed upon vertebrates, they can also act as efficient samplers that facilitate detection of vertebrate species that utilize urban ecosystems. In this study, we analyzed DNA extracted from mosquito blood meals collected temporally in multiple neighborhoods of …

Effects Of Nonlinearity And Network Architecture On The Performance Of Supervised Neural Networks, Nalinda Kulathunga, Nishath Rajiv Ranasinghe, Daniel Vrinceanu, Zackary Kinsman, Lei Huang, Yunjiao Wang

Faculty Publications

The nonlinearity of activation functions used in deep learning models is crucial for the success of predictive models. Several simple nonlinear functions, including Rectified Linear Unit (ReLU) and Leaky-ReLU (L-ReLU) are commonly used in neural networks to impose the nonlinearity. In practice, these functions remarkably enhance the model accuracy. However, there is limited insight into the effects of nonlinearity in neural networks on their performance. Here, we investigate the performance of neural network models as a function of nonlinearity using ReLU and L-ReLU activation functions in the context of different model architectures and data domains. We use entropy as a …

Transepithelial Anti-Neuroblastoma Response To Kale Among Four Vegetable Juices Using In Vitro Model Co-Culture System, John Piletz, Yuhan Mao, Debarshi Roy, Bilal Qizilbash, Eurielle Nkamssi, Enleyona Weir, Jessica Graham, Mary Emmanuel, Suwaira Iqbal, Kellie Brue, Bidisha Roy Sengupta

Faculty Publications

Juicing vegetables is thought to be an anticancer treatment. Support exists for a rank order of anticancer greens (kale > dandelion > lettuce > spinach) based on degrees of bioavailability of different phytochemicals, also offset by some noxious molecules (i.e., calcium-oxalate). We developed a new in vitro transepithelial anti-neuroblastoma model system. The juices were diluted as predicted once in the small intestine. They were applied to apical Caco-2Bbe1 cells atop dividing SH-SY5Y neuroblastoma cells, and changes in transepithelial electrical resistance (TEER) and cell growth were considered with juice spectroscopies. Studied first in monoculture, kale and dandelion were the most cytostatic juices on SH-SY5Ys, …

Documentation Of Atlantic Tarpon (Megalops Atlanticus) Space Use And Move Persistence In The Northern Gulf Of Mexico Facilitated By Angler Advocates, J. Marcus Drymon, Matthew B. Jargowsky, Michael A. Dance, Mitchell Lovell, Crystal Lou Allen Hightower, Amanda E. Jefferson, Andrea M. Kroetz, Sean P. Powers

Faculty Publications

Atlantic tarpon (Megalops atlanticus, hereafter tarpon) are facing a multitude of stressors and are considered Vulnerable by the IUCN; however, significant gaps remain in our understanding of tarpon space use and movement. From 2018 to 2019, citizen scientists facilitated tagging of 23 tarpon with SPOT tags to examine space use and movement across the northern Gulf of Mexico. Movement-based kernel densities were used to estimate simplified biased random bridge-based utilization distributions and a joint move persistence model was used to estimate a behavioral index for each fish. Tarpon showed consistent east–west movement from the Alabama/Florida border to Louisiana, and utilization …

Use Of Taxonomic And Trait-Based Approaches To Evaluate The Effect Of Bt Maize Expressing Cry1ie Protein On Non-Target Collembola: A Case Study In Northeast China, Bai Feng Wang, Feng Ci Wu, Jun Qi Yin, Zhi Lei Jiang, Xin Yuan Song, Gadi V.P. Reddy

Faculty Publications

To evaluate the effect of Bt maize expressing Cry1Ie protein on non-target soil Collembola, a two-year field study was conducted in Northeast China. Bt maize line IE09S034 and its near isoline Zong 31 were selected as experimental crops; we investigated the collembolan community using both taxonomic and trait-based approaches, and elucidated the relationship between environmental variables and the collembolan community using redundancy analysis (RDA).The ANOVA results showed that maize variety neither had significant effect on the parameters based on taxonomic approach (abundance, species richness, Shannon–Wiener index, Pielou’s evenness index), nor on the parameters based on trait-based approach (ocelli number, body …

Characterization Of The Spatial Distribution Of Alfalfa Weevil, Hypera Postica, And Its Natural Enemies, Using Geospatial Models, Govinda Shrestha, Jhalendra P. Rijal, Gadi V.P. Reddy

Faculty Publications

BACKGROUND: Understanding the spatio-temporal dynamics of prey and predator distributions can provide valuable insights into pest management strategies and conservation of natural enemies in agro-ecosystems. The alfalfa weevil, Hypera postica (Gyllenhal), is an economically important pest of alfalfa throughout the western United States. Coccinellids and nabids are among the most important natural enemies of this species, contributing to the biological control of H. postica in alfalfa fields. The spatio-temporal dynamics of H. postica and these two predator groups were investigated using 81 (= 9 × 9 grid) sample points in each of five alfalfa fields in north-central Montana. The data …

A Direct Measure Of Stand Density Based On Stand Growth, Thomas J. Dean, Anthony W. D'Amato, Brian J. Palik, Mike A. Battaglia, Constance A. Harrington

Faculty Publications

Standardizing gross volume increment on periodic height increment of the dominant trees is a means of minimizing the effects of site quality and age in growth-growing-stock relations; however, volume increment per height increment contains more information than just a normalization method for fitting growth models. This study builds on previous work suggesting that the cumulative sum of the ratios between individual-tree volume increment and height increment may be a direct measure of stand density. We used data from several levels of growing-stock studies for Douglas-fir, ponderosa pine, and red pine to explore this hypothesis. Regression analysis indicated that the sum …

Disturbed Amazonian Forests Support Diminished Breeding Bird Communities, Cameron L. Rutt, Michael D. Kaller, Philip C. Stouffer

Faculty Publications

Twenty percent of the Brazilian Amazon has now been deforested, and deforestation rates are increasing. This compels us to evaluate the conservation potential of human-modified landscapes; yet the ecological value of regenerating and fragmented Amazonian forests remains poorly understood. To date, most faunal studies in disturbed forests have examined metrics derived from presence or abundance. Although valuable, these data cannot tell us how old-growth species are using disturbed forests. In this study, we complement presence data with breeding observations to assess more comprehensively the habitat quality of disturbed forests in central Amazonia. We synthesized breeding evidence from standardized passive mist-netting, …

Increased Mood Disorder Symptoms, Perceived Stress, And Alcohol Use Among College Students During The Covid-19 Pandemic, Nora E. Charles, Stephanie J. Strong, Lauren C. Burns, Margaret R. Bullerjahn, Katherine M. Serafine

Faculty Publications

The COVID-19 pandemic caused significant disruption during the spring of 2020. Many college students were told to leave campus at spring break and to complete the semester remotely. This study evaluates effects of this disruption on student well-being. Measures of psychological symptoms, perceived stress, and alcohol use during the pandemic were completed by 148 students in spring 2020 and 352 students in fall 2020 at a university in the southeastern U.S. Results from both cohorts were compared to 240 students who completed the same measures in the fall 2019 semester. Participants in spring 2020 reported more mood disorder symptoms, perceived …

A Synthesis Of Deepwater Horizon Impacts On Coastal And Nearshore Living Marine Resources, Steven A. Murawski, Joshua P. Kilborn, Adriana C. Bejarano, David Chagaris, David Donaldson, Frank J. Hernandez, Timothy C. Macdonald, Craig Newton, Ernst Peebles, Kelly L. Robinson

Faculty Publications

The 2010 Deepwater Horizon (DWH) oil blowout in the Gulf of Mexico began on April 20, originating in the deep sea 66 km off the Louisiana coast. By early June, DWH oil had spread to coastal Louisiana, Mississippi, Alabama and western Florida. An estimated 2,113 km of shoreline were oiled, making DWH the largest marine oil spill in global history by length of affected shoreline. Additionally, a series of oil spill response measures were deployed, including diversions of Mississippi River discharge to forestall oil coming ashore, and the establishment of large-scale fishery closures, with both affecting coastal resources to varying …

Biophysical Characteristics Of Lipid-Induced Aβ Oligomers Correlate To Distinctive Phenotypes In Transgenic Mice, Jhinuk Saha, Dexter N. Dean, Shailendra Dhakal, Kelli A. Stockmal, Sarah E. Morgan, Kristy D. Dillon, Munir F. Adamo, Yona Levites, Vijayaraghavan Rangachari

Faculty Publications

Alzheimer's disease (AD) is a progressive neurodegenerative disorder that affects cognition and memory. Recent advances have helped identify many clinical sub-types in AD. Mounting evidence point toward structural polymorphism among fibrillar aggregates of amyloid-β (Aβ) to being responsible for the phenotypes and clinical manifestations. In the emerging paradigm of polymorphism and prion-like propagation of aggregates in AD, the role of low molecular weight soluble oligomers, which are long known to be the primary toxic agents, in effecting phenotypes remains inconspicuous. In this study, we present the characterization of three soluble oligomers of Aβ42, namely 14LPOs, 16LPOs, and GM1Os with discreet …

Landscape Performance Of Ebony Crepe Myrtle Cultivars In Low-Input Landscapes In North-Central Texas, Derald A. Harp, Kevin Chretien, Mariah Brown, Curtis Jones, Jose Lopez

Faculty Publications

The Ebony series of crepe myrtle (Lagerstroemia indica) cultivars includes several (Ebony Embers, Ebony Fire, Ebony Flame, Ebony Glow, and Ebony and Ivory), marketed today under the Black Diamond® brand. These are relatively new crepe myrtle cultivars unique for their dark foliage, but with little information concerning their performance in north-central Texas, especially in low-input landscapes. The study was conducted from 2014 to 2017 at three locations in north-central Texas with three soil types, an acidic fine sandy loam, a neutral pH silt loam, and an alkaline heavy clay. Although soils and environmental conditions caused variations between sites, …

Click Conjugation Of Boron Dipyrromethene (Bodipy) Fluorophores To Egfr-Targeting Linear And Cyclic Peptides, Tyrslai M. Williams, Nichole E.M. Kaufman, Zehua Zhou, Sitanshu S. Singh, Seetharama D. Jois, Maria Da Graça H. Vicente

Faculty Publications

Through a simple 1,3-cycloaddition reaction, three BODIPY-peptide conjugates that target the extracellular domain of the epidermal growth factor receptor (EGFR) were prepared and their ability for binding to EGFR was investigated. The peptide ligands K(N3)LARLLT and its cyclic analog cyclo(K(N3)larllt, previously shown to have high affinity for binding to the extracellular domain of EGFR, were conjugated to alkynyl-functionalized BODIPY dyes 1 and 2 via a copper-catalyzed click reaction. This reaction produced conjugates 3, 4, and 5 in high yields (70-82%). In vitro studies using human carcinoma HEp2 cells that overexpress EGFR demonstrated high cellular uptake, particularly for the cyclic peptide …

Anemia Management In Rural Haitian Children: A Mixed Methods Study, Marc-Aurel Martial, Kathy A. Sward, Janice M. Morse, Andrew R. Wilson, Cempaka S. Martial, Debra S. Penney, Elie Nicolas

Faculty Publications

Introduction: We examined factors influencing anemia outcomes in rural children following implementation of a prevention program. Method: Mixed methods study of children, parents, and clinicians utilized statistical modeling and content/ ethnographic analysis. Retrospective chart abstraction evaluated treatments administered and measured hemoglobin in children aged 6 to 59 months (n = 161). Prospective interviews/questionnaires examined parent (n = 51) and clinician (n = 19) perceptions. Results: Anemia prevalence decreased by 21.2%. Predictors of increased hemoglobin were clinic visit number and age at first visit. Once anemia improved, children were likely to remain improved (P = .65). Despite favorable program perceptions, stakeholders …

Linking Genes To Traits In Fungi, A. L. Romero-Olivares, Eric W. Morrison, A. Pringle, Serita D. Frey

Faculty Publications

Fungi are mediators of the nitrogen and carbon cycles in terrestrial ecosystems. Examining how nitrogen uptake and organic matter decomposition potential differs in fungi can provide insight into the underlying mechanisms driving fungal ecological processes and ecosystem functioning. In this study, we assessed the frequency of genes encoding for specific enzymes that facilitate nitrogen uptake and organic matter decomposition in 879 fungal genomes with fungal taxa grouped into trait-based categories. Our linked gene-trait data approach revealed that gene frequencies vary across and within trait-based groups and that trait-based categories differ in trait space. We present two examples of how this …

Patterns Of Relative And Quantitative Abundances Of Marine Bacteria In Surface Waters Of The Subtropical Northwest Pacific Ocean Estimated With High-Throughput Quantification Sequencing, Jie Kong, Xin Liu, Lei Wang, Hao Huang, Danyun Ou, Jiayu Guo, Edward A. Laws, Bangqin Huang

Faculty Publications

Bacteria play a pivotal role in shaping ecosystems and contributing to elemental cycling and energy flow in the oceans. However, few studies have focused on bacteria at a trans-basin scale, and studies across the subtropical Northwest Pacific Ocean (NWPO), one of the largest biomes on Earth, have been especially lacking. Although the recently developed high-throughput quantitative sequencing methodology can simultaneously provide information on relative abundance, quantitative abundance, and taxonomic affiliations, it has not been thoroughly evaluated. We collected surface seawater samples for high-throughput, quantitative sequencing of 16S rRNA genes on a transect across the subtropical NWPO to elucidate the distribution …

Associations Of Maternal Dietary Inflammatory Potential And Quality With Offspring Birth Outcomes: An Individual Participant Data Pooled Analysis Of 7 European Cohorts In The Alphabet Consortium, Ling-Wei Chen, Adrien M. Aubert, Nitin Shivappa, Jonathan Y. Bernard, Sara M. Mensink-Bout, Aisling A. Geraghty, John Mehegan, Matthew Suderman, Kinga Polanska, Wojciech Hanke, Elzbieta Trafalska, Caroline L. Relton, Sarah R. Crozier, Nicholas C. Harvey, Cyrus Cooper, Liesbeth Duijts, Barbara Heude, James R. Hébert Scd, Fionnuala M. Mcauliffe, Cecily C. Kelleher, Catherine M. Phillips

Faculty Publications

Background

Adverse birth outcomes are major causes of morbidity and mortality during childhood and associate with a higher risk of noncommunicable diseases in adult life. Maternal periconception and antenatal nutrition, mostly focusing on single nutrients or foods, has been shown to influence infant birth outcomes. However, evidence on whole diet that considers complex nutrient and food interaction is rare and conflicting. We aim to elucidate the influence of whole-diet maternal dietary inflammatory potential and quality during periconceptional and antenatal periods on birth outcomes.

Methods and findings

We harmonized and pooled individual participant data (IPD) from up to 24,861 mother–child pairs …

Effects Of Spatial Variability On The Exposure Of Fish To Hypoxia: A Modeling Analysis For The Gulf Of Mexico, Elizabeth D. Labone, Kenneth A. Rose, Dubravko Justic, Haosheng Huang, Lixia Wang

Faculty Publications

The hypoxic zone in the northern Gulf of Mexico varies spatially (area, location) and temporally (onset, duration) on multiple scales. Exposure of fish to hypoxic dissolved oxygen (DO) concentrations (

Trends In Growth Modeling In Fisheries Science, Shane A. Flinn, Stephen R. Midway

Faculty Publications

No abstract provided.

Topsentinol L Trisulfate, A Marine Natural Product That Targets Basal-Like And Claudin-Low Breast Cancers, Nader N. El-Chaar, Thomas E. Smith, Gajendra Shrestha, Stephen R. Piccolo, Mary Kay Harper, Ryan M. Van Wagoner, Zhenyu Lu, Ashlee R. Venancio, Chris M. Ireland, Andrea H. Bild, Philip J. Moos

Faculty Publications

Patients diagnosed with basal-like breast cancer suffer from poor prognosis and limited treatment options. There is an urgent need to identify new targets that can benefit patients with basal-like and claudin-low (BL-CL) breast cancers. We screened fractions from our Marine Invertebrate Compound Library (MICL) to identify compounds that specifically target BL-CL breast cancers. We identified a previously unreported trisulfated sterol, i.e., topsentinol L trisulfate (TLT), which exhibited increased efficacy against BL-CL breast cancers relative to luminal/HER2+ breast cancer. Biochemical investigation of the effects of TLT on BL-CL cell lines revealed its ability to inhibit activation of AMP-activated protein kinase (AMPK) …

3d-Printed Microfluidic Droplet Generator With Hydrophilic And Hydrophobic Polymers, Chandler A. Warr, Hunter S. Hinnen, Saroya Avery, Rebecca J. Cate, Gregory P. Nordin, William G. Pitt

Faculty Publications

Droplet generation has been widely used in conventional two-dimensional (2D) microfluidic devices, and has recently begun to be explored for 3D-printed droplet generators. A major challenge for 3D-printed devices is preventing water-in-oil droplets from sticking to the interior surfaces of the droplet generator when the device is not made from hydrophobic materials. In this study, two approaches were investigated and shown to successfully form droplets in 3D-printed microfluidic devices. First, several printing resin candidates were tested to evaluate their suitability for droplet formation and material properties. We determined that a hexanediol diacrylate/lauryl acrylate (HDDA/LA) resin forms a solid polymer that …

3d-Printed Microfluidic Droplet Generator With Hydrophilic And Hydrophobic Polymers, Chandler A. Warr, Hunter S. Hinnen, Saroya Avery, Rebecca J. Cate, Gregory P. Nordin, William G. Pitt

Faculty Publications

Droplet generation has been widely used in conventional two-dimensional (2D) microfluidic devices, and has recently begun to be explored for 3D-printed droplet generators. A major challenge for 3D-printed devices is preventing water-in-oil droplets from sticking to the interior surfaces of the droplet generator when the device is not made from hydrophobic materials. In this study, two approaches were investigated and shown to successfully form droplets in 3D-printed microfluidic devices. First, several printing resin candidates were tested to evaluate their suitability for droplet formation and material properties. We determined that a hexanediol diacrylate/lauryl acrylate (HDDA/LA) resin forms a solid polymer that …

The Barley Hvwrky6 Transcription Factor Is Required For Resistance Against Pyrenophora Teres F. Teres, Prabin Tamang, Jonathan K. Richards, Shyam Solanki, Gazala Ameen, Roshan Sharma Poudel, Priyanka Deka, Karl Effertz, Shaun J. Clare, Justin Hegstad, Achintya Bezbaruah, Xuehui Li, Richard D. Horsley, Timothy L. Friesen, Robert S. Brueggeman

Faculty Publications

Barley is an important cereal crop worldwide because of its use in the brewing and distilling industry. However, adequate supplies of quality malting barley are threatened by global climate change due to drought in some regions and excess precipitation in others, which facilitates epidemics caused by fungal pathogens. The disease net form net blotch caused by the necrotrophic fungal pathogen Pyrenophora teres f. teres (Ptt) has emerged as a global threat to barley production and diverse populations of Ptt have shown a capacity to overcome deployed genetic resistances. The barley line CI5791 exhibits remarkably effective resistance to diverse Ptt isolates …

Identify Rna-Associated Subcellular Localizations Based On Multi-Label Learning Using Chou’S 5-Steps Rule, Hao Wang, Yijie Ding, Jijun Tang Ph.D., Quan Zou, Fei Guo

Faculty Publications

Background: Biological functions of biomolecules rely on the cellular compartments where they are located in cells. Importantly, RNAs are assigned in specific locations of a cell, enabling the cell to implement diverse biochemical processes in the way of concurrency. However, lots of existing RNA subcellular localization classifiers only solve the problem of single-label classification. It is of great practical significance to expand RNA subcellular localization into multi-label classification problem.

Results: In this study, we extract multi-label classification datasets about RNA-associated subcellular localizations on various types of RNAs, and then construct subcellular localization datasets on four RNA categories. In order to …