Digital Commons Network^™

Enhancing Pastoralist Women's Knowledge Of Danger Signs Through Home-Based Life-Saving Skills Intervention In Northern Kenya: A Quasi Experimental Study, Dabo Galgalo Halake, Elijah Isinta Maranga, Japheth Mativo Nzioki, John Gachohi

Faculty Publications

Background: Maternal recognition of obstetric danger signs is crucial for reducing maternal mortality and delays in seeking emergency care. However, there is insufficient knowledge about obstetric danger signs among women in rural Kenya, especially in the hard-to-reach pastoralist communities. This study aimed to determine whether home-based life-saving skills intervention improves knowledge about obstetric danger signs among women in Marsabit County, Kenya. Methods: We conducted a quasi-experimental study with pre-posttests among 256 pregnant women, allocated to intervention and control groups. We implemented sensitization of pregnant women on obstetric danger signs and basic life-saving actions as components of home-based life-saving skills intervention. …

New Features Of Laboratory-Generated Epfrs From 1,2-Dichlorobenzene (Dcb) And 2-Monochlorophenol (Mcp), Lavrent Khachatryan, Marwan Y. Rezk, Divine Nde, Farhana Hasan, Slawomir Lomnicki, Dorin Boldor, Robert Cook, Phillip Sprunger, Randall Hall, Stephania Cormier

Faculty Publications

The present research is primarily focused on investigating the characteristics of environmentally persistent free radicals (EPFRs) generated from commonly recognized aromatic precursors, namely, 1,2-dichlorobenzene (DCB) and 2-monochlorophenol (MCP), within controlled laboratory conditions at a temperature of 230 °C, termed as DCB230 and MCP230 EPFRs, respectively. An intriguing observation has emerged during the creation of EPFRs from MCP and DCB utilizing a catalyst 5% CuO/SiO2, which was prepared through various methods. A previously proposed mechanism, advanced by Dellinger and colleagues (a conventional model), postulated a positive correlation between the degree of hydroxylation on the catalyst’s surface (higher hydroxylated, HH and less …

Canada's Special Economic Measures Act Under International Law, Preston Jordan Lim

Faculty Publications

No abstract provided.

Predictors Of Prior Hiv Testing And Acceptance Of A Community-Based Hiv Test Offer Among Male Bar Patrons In Northern Tanzania, Deng B. Madut, Preeti Manavalan, Antipas Mtalo, Timothy Peter, Jan Ostermann, Bernard Njau, Nathan M. Thielman

Faculty Publications

Community-based HIV testing offers an alternative approach to encourage HIV testing among men in sub-Saharan Africa. In this study, we evaluated a community-based HIV testing strategy targeting male bar patrons in northern Tanzania to assess factors predictive of prior HIV testing and factors predictive of accepting a real-time HIV test offer. Participants completed a detailed survey and were offered HIV testing upon survey completion. Poisson regression was used to identify prevalence ratios for the association between potential predictors and prior HIV testing or real-time testing uptake. Of 359 participants analyzed, the median age was 41 (range 19–82) years, 257 (71.6%) …

Does Chinese Investment Into Europe Facilitate Strategic Asset Growth In The Chinese Parent Company? The Role Of Entry Mode, John Anderson, Dylan Sutherland, Sean Severe

Faculty Publications

Strategic asset seeking foreign direct investment has undergone tremendous growth over the past decade. This paper first attempts to evaluate the location choice of such investments in Europe. We find that Chinese companies target strategic assets in Europe. The paper then moves to understand the efficacy of these investments in terms of the creation of strategic assets in the Chinese parent company. Our results show the intangible assets of Chinese domestic parent firms significantly increase in the wake of their investments. For greenfield investments, there is a longer time-lag in creation of intangible strategic assets than for acquisitions. However, greenfield …

Observational Features Of Charge Distribution In Earth's Inner Magnetosphere, Lai Gao, Chao Shen, Yufei Zhou, Yong Ji, Zuyin Pu, George Parks, Christopher T. Russell, Gang Zeng, Lan Ma, Roy B. Torbert, Yanyan Yang, James L. Burch

Faculty Publications

Understanding the motion of charged particles in the electromagnetic field in the inner magnetosphere is essential for space science and space weather. Charge accumulation can occur due to the dipole magnetic and convective electric fields in this region. However, until the recent Magnetospheric Multiscale (MMS) mission, there were few means to detect charge distribution in situ. We report unambiguous in situ observation of the spatial distribution of the excess charge density in the inner magnetosphere by the MMS mission. We find that a positive (negative) charge accumulates in the dusk (dawn) side inner magnetospheres, which is contrary to the long …

Observational Features Of Charge Distribution In Earth's Inner Magnetosphere, Lai Gao, Chao Shen, Yufei Zhou, Yong Ji, Zuyin Pu, George Parks, Christopher T. Russell, Gang Zeng, Lan Ma, Roy B. Torbert, Yanyan Yang, James L. Burch

Faculty Publications

Understanding the motion of charged particles in the electromagnetic field in the inner magnetosphere is essential for space science and space weather. Charge accumulation can occur due to the dipole magnetic and convective electric fields in this region. However, until the recent Magnetospheric Multiscale (MMS) mission, there were few means to detect charge distribution in situ. We report unambiguous in situ observation of the spatial distribution of the excess charge density in the inner magnetosphere by the MMS mission. We find that a positive (negative) charge accumulates in the dusk (dawn) side inner magnetospheres, which is contrary to the long …

Improving The Practical Skills Of Stem Students At A Historically Black College And University (Hbcu), Yasser Ismail, Phyllis Okwan, Fred Lacy

Faculty Publications

Improving the practical skills of Science, Technology, Engineering and Mathematics (STEM) students at a historically black college and university (HBCU) was done by implementing a transformative teaching model. The model was implemented on undergraduate students of different educational levels in the Electrical Engineering (EE) Department at HBCU. The model was also extended to carefully chosen high and middle schools. These middle and high school students serve as a pipeline to the university, with a particular emphasis on fostering growth within the EE Department. The model aligns well with the core mission of the EE Department, aiming to enhance the theoretical …

The Mediating Effect Of The Covid-19 Pandemic On Heuristic Techniques And Cognitive Biases On Investment Decision-Making, Williams Kwasi Peprah, Christopher Ampadu Kwakwah-Oppong, Francis Osei-Kuffour, Lenny Leorina Evinita

Faculty Publications

US investors’ behavioral finance and investment decision-making approach became a concern during the COVID-19 pandemic era as the capital market crashed. The study used mediation analysis to explain how or why the COVID-19 pandemic intervened in the causal association of behavioral finance concepts of heuristic techniques and cognitive biases on their investment decision-making. This causal research design study was based on 500 snowball-sampled US investors who answered self-constructed, validated, and reliability-tested Likert-scale quantitative variables measured through a first-party data collection approach. The results showed that the COVID-19 pandemic was a specific moderate to partial significant mediator on the low positive …

Heavy-Element Production In A Compact Object Merger Observed By Jwst, Andrew J. Levan, Benjamin P. Gompertz, Om Sharan Salafia, Mattia Bulla, Eric Burns, Kenta Hotokezaka, Luca Izzo, Gavin P. Lamb, Daniele B. Malesani, Samantha R. Oates, Maria Edvige Ravasio, Alicia Rouco Escorial, Benjamin Schneider, Nikhil Sarin, Steve Schulze, Nial R. Tanvir, Kendall Ackley, Gemma Anderson, Gabriel B. Brammer, Lise Christensen, Vikram S. Dhillon, Phil A. Evans, Michael Fausnaugh, Wen Fai Fong, Andrew S. Fruchter, Chris Fryer, Johan P.U. Fynbo, Nicola Gaspari, Kasper E. Heintz, Jens Hjorth, Jamie A. Kennea, Mark R. Kennedy, Tanmoy Laskar

Faculty Publications

The mergers of binary compact objects such as neutron stars and black holes are of central interest to several areas of astrophysics, including as the progenitors of gamma-ray bursts (GRBs)¹, sources of high-frequency gravitational waves (GWs)² and likely production sites for heavy-element nucleosynthesis by means of rapid neutron capture (the r-process)³. Here we present observations of the exceptionally bright GRB 230307A. We show that GRB 230307A belongs to the class of long-duration GRBs associated with compact object mergers^4–6 and contains a kilonova similar to AT2017gfo, associated with the GW merger GW170817 (refs. ^7–12). …

Detecting Substance Use Disorder Using Social Media Data And Dark Web: Time And Knowledge Aware Study, Usha Lokala, Orchid Chetia Phukan, Triyasha Ghosh Dastidar, Francois Lamy, Raminta Daniulaityte, Amit Sheth

Faculty Publications

Opioid and substance misuse is rampant in the United States today, with the phenomenon known as the "opioid crisis". The relationship between substance use and mental health has been extensively studied, with one possible relationship being: substance misuse causes poor mental health. However, the lack of evidence on the relationship has resulted in opioids being largely inaccessible through legal means. This study analyzes the substance use posts on social media with opioids being sold through crypto market listings. We use the Drug Abuse Ontology, state-of-the-art deep learning, and knowledge-aware BERT-based models to generate sentiment and emotion for the social media …

Overview Of The Alaskan Layered Pollution And Chemical Analysis (Alpaca) Field Experiment, William R. Simpson, Jingqiu Mao, Gilberto J. Fochesatto, Kathy S. Law, Peter F. Decarlo, Julia Schmale, Kerri A. Pratt, Steve R. Arnold, Jochen Stutz, Jack E. Dibb, Jessie M. Creamean, Rodney J. Weber, Brent J. Williams, Becky Alexander, Lu Hu, Robert J. Yokelson, Manabu Shiraiwa, Stefano Decesari, Cort Anastasio, Barbara D’Anna, Robert C. Gilliam, Athanasios Nenes, Jason M. St. Clair, Barbara Trost, James H. Flynn, Joel Savarino, Laura D. Conner, Nathan Kettle, Krista M. Heeringa, Sarah Albertin, Andrea Baccarini, Brice Barret, Michael A. Battaglia, Slimane Bekki, T. J. Brado, Natalie Brett, David Brus, James R. Campbell, Meeta Cesler-Maloney, Sol Cooperdock, Karolina Cysneiros De Carvalho, Hervé Delbarre, Paul J. Demott, Conor J.S. Dennehy, Elsa Dieudonné, Kayane K. Dingilian, Antonio Donateo, Konstantinos M. Doulgeris, Kasey C. Edwards, Kathleen Fahey, Ting Fang, Fangzhou Guo, Laura M. D. Heinlein, Andrew L. Holen, Deanna Huff, Amna Ijaz, Sarah Johnson, Sukriti Kapur, Damien T. Ketcherside, Ezra Levin, Emily Lill, Allison R. Moon, Tatsuo Onishi, Gianluca Pappaccogli, Russell Perkins, Roman Pohorsky, Jean-Christophe Raut, Francois Ravetta, Tjarda Roberts, Ellis S. Robinson, Federico Scoto, Vanessa Selimovic, Michael O. Sunday, Brice Temime-Roussel, Xinxiu Tian, Judy Wu, Yuhan Yang

Faculty Publications

The Alaskan Layered Pollution And Chemical Analysis (ALPACA) field experiment was a collaborative study designed to improve understanding of pollution sources and chemical processes during winter (cold climate and low-photochemical activity), to investigate indoor pollution, and to study dispersion of pollution as affected by frequent temperature inversions. A number of the research goals were motivated by questions raised by residents of Fairbanks, Alaska, where the study was held. This paper describes the measurement strategies and the conditions encountered during the January and February 2022 field experiment, and reports early examples of how the measurements addressed research goals, particularly those of …

Comparative Methods For Teaching Contemporary And Ancient Saints, Todd E. French, Mohammed Forero Bucheli

Faculty Publications

This paper argues that a comparative study of saints is not only a useful classroom tool for historians and religionists, but an exceptionally powerful locus of pedagogical insight and cultural understanding. By reframing contemporary consumptive patterns, media representations, and power discourses as religious vectors of saintliness, the professor has an opportunity to explore and assess cultural values, rituals, beliefs, worldviews, communities, traditions, and meaning making in the contemporary college student’s world. By acknowledging the dangers and possibilities of the category of saint while reframing the ascetical impact on developing subjectivities, we propose six pedagogical examples of how this might best …

Identification Of Potential Therapist “Missteps” In The Context Of Engagement In Public Mental Health Services: Analysis Of Digital Recordings And Key Informant Validation, Hyun Seon Park, Kimberly Becker, Wendy Chu, Kendal Reeder, Carolina Lechuga, Johnathan K. Ahuna, Eleanor Wu, Lanalle Darden, Rosalyn Williams, Rosana Serrano, Bruce F. Chorpita

Faculty Publications

Objective: This study aimed to characterize and validate potential therapist “missteps,” or inadvertent behaviors that may impact youth and family engagement in mental health services.

Method: Ten misstep types were coded from 224 treatment sessions provided by 92 therapists. Rapid qualitative analysis using key informant interviews was conducted to examine insights related to the occurrence, expected impact, and perceived severity of therapist missteps as an indicator of the face validity of the missteps construct and types.

Results: All 10 misstep types demonstrated good reliability (Fleiss’ k = .71−.88). All 10 misstep types appeared to have facevalidity with key informants. Key …

Effects Of Adjunct Questions On L2 Reading Comprehension With Texts Of Different Types, Yunmei Sun, Wenhui Zhou, Shifang Tang

Faculty Publications

Answering text-related questions while reading is a questioning strategy which is called adjunct questions or embedded questions, the benefits of which have been established in first language reading as to enhance comprehension. The present study aims to study the effects different adjunct questions exert on second-language (L2) readers’ comprehension of texts of various types. One hundred and forty-four intermediate-level Chinese EFL learners participated in this study and were divided randomly into six groups. Each group was given either a narrative or an expository text with ‘what or why’ questions or no questions. A brief topic familiarity questionnaire was attached to …

Food Systems Under Shock: The Evolution Of Food Security Status Of Karantina Residents After The Beirut Explosion, Mariana Makoukji, Nour El Houda Amhez, Abed Al Kareem Yehya, Hala Ghattas Ph.D., Yaser Abunnasr, Rami Zurayk

Faculty Publications

Robust food systems are crucial for ensuring access to affordable and nutritious food, particularly in times of crisis. Economic instability, currency devaluation, and political turmoil can disrupt food systems, leading to higher food prices, compromised nutrition, and increased vulnerability. Lebanon is a stark example of this, where a catastrophic explosion in Beirut’s Port in 2020 worsened an existing economic and humanitarian crisis. The depreciation of the Lebanese currency led to soaring food prices, impacting people’s ability to buy food, and pushing many into poverty, illustrating the significant impact of the state food systems during crises (FAO, 2021). In times of …

Children In The Workplace: An Exploration In Library Policy Making, Sharolyn Swenson, Marissa Anne Bischoff, Ryan Lee

Faculty Publications

Children in the workplace are becoming a more common discussion in various work environments, including libraries. Since the university has no policy addressing this issue, a task force was charged to draft a recommended policy for the university library regarding bringing children to the workplace. The task force reviewed existing policies and conducted a survey and interviews with library employees. The resulting policy provided guidelines for employees and their supervisors without being overly prescriptive. This article provides a case study of how the task force used assessment methods and tools to create an appropriate and inclusive policy. While the specific …

Examining The Evolution Of Network Governance Forms Of An Event Leveraging Collective: A Longitudinal Investigation, Landy Lu, Ran Zhou, Guangzhou Chen, Laura Misener

Faculty Publications

This study aims to investigate how an event leveraging collective’s network governance forms evolved from pre- to post‐Games and how these forms influenced members' collaborative engagement. We adopted a longitudinal qualitative case study approach. Our primary data sources included 996 pages of archival documents and 18 interviews with collective members. We found that in the pre- and during-Games stage, the leveraging collective adopted a shared participant-governed form with a facilitator. This participant-driven structure could enhance connectedness and collaborative engagement among member organizations. In the postevent phase, the collective assumed a pillar-governed form with a facilitator. Although this structure appeared to …

Resonance: Collaborative Musicking Through Tactile Ecologies, Ka Hei Cheng, Jesse Allison

Faculty Publications

This paper unveils a PhD research on "Resonance,"an instrument at the crossroads of collaborative music-making, gameplay, and music-related human-computer interaction. Resonance creates a unique communal gameplay performance system, where players are interdependent, transcending the boundaries of human and non-human agency. This collective process has the potential to enhance human connections and bonding, amplifying players' sensitivity to one another and their environment. The heart of this collaboration comprises intricate networks of signals, nurturing relationships, shared expression, decision-making processes, negotiation, and a spectrum of implementations. Resonance's design showcases tactile and tangible interfaces, employing capacitive touch technology as a sound interface, thereby providing …

Technical Efficiency, Scale Effect, And Trade Liberalization: Evidence From The Nepalese Manufacturing Sector, Amrit Pathak, Shawn Leu, Mari L. Robertson, Mahinda Siriwardanaa

Faculty Publications

This paper investigates the impact of trade liberalization and scale of operation on technical efficiency of the Nepalese manufacturing sector after the 1992 economic liberalization. The study utilizes industry panel data and analyses the relationship using stochastic frontier analysis. We find that the manufacturing sector of Nepal is highly inefficient. Technical progress is found initially, however after 1996/97, technical regress dominates each period. Sectoral efficiency, on average, is estimated around 28.7% of potential value added whereas inefficiency is as high as 146%. The scale of operation, as expected, is highly significant in explaining industry efficiency. Trade liberalization, on the other …

Drivers Of Anticoagulant Rodenticide Exposure In Fishers (Pekania Pennanti) Across The Northeastern United States, Georgianna Silveira, Jacqueline L. Frair, Lisa Murphy, Julie C. Ellis, David Needle, Stephanie A. Cunningham, Amanda Watson, Aaron Facka, Patrick Tate, Shevenell Webb, Kim Royar, Chris Bernier, Thomas Keller, Krysten Schuler

Faculty Publications

Studies in parts of Europe, New Zealand, and North America indicate uptake of anticoagulant rodenticides (ARs) by predatory mammals to be widespread and common, with proximity to urban and agricultural areas being an important driver of exposure. Yet, little is known regarding the patterns and drivers of AR exposure in predatory mammals within more forest-dominated landscapes. Across the forest-dominated northeastern United States (US), a region spanning Pennsylvania to Maine, we tested livers from 597 fisher (Pekania pennanti) obtained from the legal harvest for 11 anticoagulant rodenticide compounds. We used kriging to determine potential hot zones (within or among states) and …

Drivers Of Anticoagulant Rodenticide Exposure In Fishers (Pekania Pennanti) Across The Northeastern United States, Georgianna Silveira, Jacqueline L. Frair, Lisa Murphy, Julie C. Ellis, David Needle, Stephanie A. Cunningham, Amanda Watson, Aaron Facka, Patrick Tate, Shevenell Webb, Kim Royar, Chris Bernier, Thomas Keller, Krysten Schuler

Faculty Publications

Studies in parts of Europe, New Zealand, and North America indicate uptake of anticoagulant rodenticides (ARs) by predatory mammals to be widespread and common, with proximity to urban and agricultural areas being an important driver of exposure. Yet, little is known regarding the patterns and drivers of AR exposure in predatory mammals within more forest-dominated landscapes. Across the forest-dominated northeastern United States (US), a region spanning Pennsylvania to Maine, we tested livers from 597 fisher (Pekania pennanti) obtained from the legal harvest for 11 anticoagulant rodenticide compounds. We used kriging to determine potential hot zones (within or among states) and …

Advancing Cr(Vi) Electroreduction: A Redox Mediator To Catalyze The Electrochemical Reduction Of Cr(Vi) In Water While Preventing Fouling Of Carbon Electrodes, Callie M. Stern, Malithi M. Abeythunga, Noémie Elgrishi

Faculty Publications

Hexavalent chromium is a contaminant of concern and is found in drinking water supplies. Electrochemical methods are well-suited to accomplish the reduction of toxic Cr(VI) to Cr(III). However, high overpotentials and plating of Cr(III) products on electrodes have stymied the development of efficacious purification methods. The Cr(VI) reduction reaction necessitates the transfer of multiple protons and electrons, which is accompanied by a high kinetic barrier. Following recent advances in the electrocatalytic energy storage community, we report that the use of [Fe(CN)6]^3- as a small molecular electrocatalyst not only diminishes the overpotential for Cr(VI) reduction on carbon electrodes by 0.575 …

Experimental Determination Of The Electrochemical Properties Of Bismuth Chloride In Eutectic Licl-Kcl And Licl-Kcl-Cacl2 Molten Salts, Greg Chipman, Bryant Johnson, Cameron Vann, Lance Whitesides, Devin Rappleye

Faculty Publications

Bismuth has been investigated as a potential liquid electrode for molten salt electrorefining, but the electrochemical behavior of Bi³⁺/Bi redox couple has received scant attention in eutectic LiCl-KCl melts and no studies were found in the ternary LiCl-KCl-CaCl₂ melts. LiCl-KCl-CaCl₂ melts offer some advantages over eutectic LiCl-KCl, such as a lower melting point. Cyclic voltammetry, square wave voltammetry, chronoamperometry, chronopotentiometry and open-circuit chronopotentiometry were used to measure electrochemical parameters, such as diffusivity and standard apparent potential of bismuth electrodeposition, in LiCl-KCl and LiCl-KCl-CaCl₂ eutectics. The mean diffusivity values of 6.71 x 10^-6 cm^{2 …}

Sexual Orientation And Gender Identity Measures And Viral Suppression For People Living With Hiv: A Protocol For A Population-Based Cohort Study, Xueying Yang, Bankole Olatosi Ph.D., Sharon Weissman, Xiaoming Li Ph.D., Jiajia Zhang Ph.D.

Faculty Publications

Introduction The measure of sexual orientation and gender identity (SOGI) data in electronic health records (EHR) has been critical for addressing health disparities and inequalities, especially for HIV care. Given that gender and sexual minorities (eg, transgender, men who have sex with men and intersex) are key groups in people living with HIV (PLWH), SOGI data can facilitate a more accurate understanding about the HIV outcomes (eg, viral suppression) among this key group and then lead to tailored therapeutic services. The two-step SOGI collection method as an emerging gender measurement can be used to measure SOGI status in medical settings. …

Hybrid Interpersonal Violence In Latin America: Causes And Patterns, Abigail Weitzman, Mónica Caudillo, Eldad J. Levy

Faculty Publications

In this review, we argue that to understand patterns and causes of violence in contemporary Latin America, we must explicitly consider when violence takes on interpersonal qualities. We begin by reviewing prominent definitions and measurements of interpersonal violence. We then detail the proliferation of interlocking sources of regional insecurity, including gender-based violence, gangs, narcotrafficking, vigilantism, and political corruption. Throughout this description, we highlight when and how each source of insecurity can become interpersonal. Next, we outline mutually reinforcing macro and micro conditions underlying interpersonal violence in its many hybrid forms. To conclude, we call for more multifaceted conceptualizations of interpersonal …

Creating An Inclusive Metadata Policy, Nicole Lewis, Jeremy Myntti

Faculty Publications

Best of Core Forum webinar series

The BYU Library has created an Inclusive Metadata Policy for metadata creators to use when creating and remediating descriptions to be more inclusive. Accompanying the policy is a companion document with recommendations and examples, including links to many external resources. In addition to working with stakeholders in the library to develop the policy, we consulted with the University’s Office of Belonging. This presentation describes the process we used to undertake the creation of the library policy and recommendations document.

Racialized Early Grade (Mis)Behavior: The Links Between Same-Race/Ethnicity Teachers And Discipline In Elementary School, Nayoung Hwang, Patrick Graff, Mark Berends

Faculty Publications

Studies persistently show disparities in exclusionary discipline across racial/ethnic groups in U.S. schools. Using administrative data from kindergarteners through fifth graders in Indiana, we examine the effects of student-teacher race/ethnicity matching on disciplinary outcomes. We find that Black students exhibit lower rates of suspension and expulsion when they study with Black teachers—driven mainly by fewer defiance and profanity offenses. By contrast, for Latinx and White students, having a teacher of the same race/ethnicity is not associated with suspension and expulsion. In light of the shortage of Black teachers in the teacher workforce, our findings underscore the vulnerability of Black students …

On Personality Measures And Their Data: A Classification Of Measurement Approaches And Their Recommended Uses, John D. Mayer, Victoria M. Bryan

Faculty Publications

We employ a new approach for classifying methods of personality measurement such as self-judgment, mental ability, and lifespace measures and the data they produce. We divide these measures into two fundamental groups: personal-source data, which arise from the target person’s own reports, and external-source data, which derive from the areas surrounding the person. These two broad classes are then further divided according to what they target and the response processes that produce them. We use the model to organize roughly a dozen kinds of data currently employed in the field. With this classification system in hand, we …

High-Yield And Rapid Isolation Of Extracellular Vesicles By Flocculation Via Orbital Acoustic Trapping: Float, Joseph Rufo, Peiran Zhang, Zeyu Wang, Yuyang Gu, Kaichun Yang, Joseph Rich, Chuyi Chen, Ruoyu Zhong, Ke Jin, Je He, Jianping Xia, Ke Li, Jiarong Wu, Yingshi Ouyang, Yoel Sadovsky, Luke P. Lee, Tony Jun Huang

Faculty Publications

Extracellular vesicles (EVs) have been identified as promising biomarkers for the noninvasive diagnosis of various diseases. However, challenges in separating EVs from soluble proteins have resulted in variable EV recovery rates and low purities. Here, we report a high-yield ( > 90%) and rapid ( <  10 min) EV isolation method called FLocculation via Orbital Acoustic Trapping (FLOAT). The FLOAT approach utilizes an acoustofluidic droplet centrifuge to rotate and controllably heat liquid droplets. By adding a thermoresponsive polymer flocculant, nanoparticles as small as 20 nm can be rapidly and selectively concentrated at the center of the droplet. We demonstrate the ability of FLOAT to separate urinary EVs from the highly abundant Tamm-Horsfall protein, addressing a significant obstacle in the development of EV-based liquid biopsies. Due to its high-yield nature, FLOAT reduces biofluid starting volume requirements by a factor of 100 (from 20 mL to 200 µL), demonstrating its promising potential in point-of-care diagnostics.