Digital Commons Network^™

Egg Intake And Dietary Quality Among Overweight And Obese Mexican-American Postpartum Women, Sonia Sonia Vega-López, Giselle Pignotti, Michael Todd, Colleen Keller

Faculty Publications

Despite their low cost and high nutrient density, the contribution of eggs to nutrient intake and dietary quality among Mexican-American postpartum women has not been evaluated. Nutrient intake and dietary quality, as assessed by the Healthy Eating Index 2010 (HEI-2010), were measured in habitually sedentary overweight/obese (body mass index (BMI) = 29.7 ± 3.5 kg/m2) Mexican-American postpartum women (28 ± 6 years) and compared between egg consumers (n = 82; any egg intake reported in at least one of three 24-h dietary recalls) and non-consumers (n = 57). Egg consumers had greater intake of energy (+808 kJ (193 kcal) or …

Improving Regulatory Enforcement In The Face Of Inadequate Resources, Sharona Hoffman

Faculty Publications

This article is a teaching exercise, designed to focus students’ attention on the challenges of regulatory enforcement. It grows out of the author’s Robert Wood Johnson Foundation Public Health Law Fellowship during which she worked with the Oregon Health Authority. The case example is drawn from Oregon’s regulation of in-home care agencies (IHCA). Students are asked to formulate suggestions for enhancing compliance with IHCA regulations in the absence of additional funding. The author includes her own recommendations for positive and negative incentives for regulatory compliance, which she developed during her fellowship.

Increased Phosphate Transport Of Arabidopsis Thalianapht1;1 By Site-Directed Mutagenesis Of Tyrosine 312 May Be Attributed To The Disruption Of Homomeric Interactions, Elena B. Fontenot, Sandra Feuer Ditusa, Naohiro Kato, Danielle M. Olivier, Renee Dale, Wei Yi Lin, Tzyy Jen Chiou, Megan A. Macnaughtan, Aaron P. Smith

Faculty Publications

& Sons Ltd. Members of the Pht1 family of plant phosphate (Pi) transporters play vital roles in Pi acquisition from soil and in plantaPi translocation to maintain optimal growth and development. The study of the specificities and biochemical properties of Pht1 transporters will contribute to improving the current understanding of plant phosphorus homeostasis and use-efficiency. In this study, we show through split in vivo interaction methods and in vitro analysis of microsomal root tissues that Arabidopsis thalianaPht1;1 and Pht1;4 form homomeric and heteromeric complexes. Transient and heterologous expression of the Pht1;1 variants, Pht1;1^Y312D, Pht1;1^Y312A and Pht1;1^{Y312F …}

Il-1Β Reciprocally Regulates Chemokine And Insulin Secretion In Pancreatic Β-Cells Via Nf-Κb, Susan J. Burke, Krisztian Stadler, Danhong Lu, Evanna Gleason, Anna Han, Dallas R. Donohoe, Richard C. Rogers, Gerlinda E. Hermann, Michael D. Karlstad, J. Jason Collier

Faculty Publications

© 2015 the American Physiological Society. Proinflammatory cytokines impact islet β-cell mass and function by altering the transcriptional activity within pancreatic β-cells, producing increases in intracellular nitric oxide abundance and the synthesis and secretion of immunomodulatory proteins such as chemokines. Herein, we report that IL-1β, a major mediator of inflammatory responses associated with diabetes development, coordinately and reciprocally regulates chemokine and insulin secretion. We discovered that NF-κB controls the increase in chemokine transcription and secretion as well as the decrease in both insulin secretion and proliferation in response to IL-1β. Nitric oxide production, which is markedly elevated in pancreatic β-cells …

Increased Phosphate Transport Of Arabidopsis Thalianapht1;1 By Site-Directed Mutagenesis Of Tyrosine 312 May Be Attributed To The Disruption Of Homomeric Interactions, Elena B. Fontenot, Sandra Feuer Ditusa, Naohiro Kato, Danielle M. Olivier, Renee Dale, Wei Yi Lin, Tzyy Jen Chiou, Megan A. Macnaughtan, Aaron P. Smith

Faculty Publications

& Sons Ltd. Members of the Pht1 family of plant phosphate (Pi) transporters play vital roles in Pi acquisition from soil and in plantaPi translocation to maintain optimal growth and development. The study of the specificities and biochemical properties of Pht1 transporters will contribute to improving the current understanding of plant phosphorus homeostasis and use-efficiency. In this study, we show through split in vivo interaction methods and in vitro analysis of microsomal root tissues that Arabidopsis thalianaPht1;1 and Pht1;4 form homomeric and heteromeric complexes. Transient and heterologous expression of the Pht1;1 variants, Pht1;1^Y312D, Pht1;1^Y312A and Pht1;1^{Y312F …}

Induced Jasmonate Signaling Leads To Contrasting Effects On Root Damage And Herbivore Performance, Jing Lu, Christelle Aurélie Maud Robert, Michael Riemann, Marco Cosme, Laurent Mène-Saffrané, Josep Massana, Michael Joseph Stout, Yonggen Lou, Jonathan Gershenzon, Matthias Erb

Faculty Publications

© 2015 American Society of Plant Biologists. All rights reserved. Induced defenses play a key role in plant resistance against leaf feeders. However, very little is known about the signals that are involved in defending plants against root feeders and how they are influenced by abiotic factors. We investigated these aspects for the interaction between rice (Oryza sativa) and two root-feeding insects: the generalist cucumber beetle (Diabrotica balteata) and the more specialized rice water weevil (Lissorhoptrus oryzophilus). Rice plants responded to root attack by increasing the production of jasmonic acid (JA) and abscisic acid, whereas in contrast to in herbivore-attacked …

Factors Influencing Expression Of Antixenosis In Soybean To Anticarsia Gemmatalis And Spodoptera Frugiperda (Lepidoptera: Noctuidae), Arlindo Leal Boiça Júnior, Bruno Henrique Sardinha De Souza, Eduardo Neves Costa, Zulene Antonio Ribeiro, Michael Joseph Stout

Faculty Publications

© The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. This study aimed to evaluate some factors that influence the expression of antixenosis in soybean genotypes against Anticarsia gemmatalis Hübner and Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae). Free-choice and no-choice feeding assays were performed with the resistant soybean genotype IAC 100 and the susceptible genotype BRSGO 8360 using A. gemmatalis and S. frugiperda larvae. The following factors that may affect expression of resistance were evaluated: one larva versus two larvae per leaf disc; use of larvae without prior feeding suspension versus larvae starved …

Role Of Resistant Starch In Improving Gut Health, Adiposity, And Insulin Resistance, Michael J. Keenan, June Zhou, Maren Hegsted, Christine Pelkman, Holiday A. Durham, Diana B. Coulon, Roy J. Martin

Faculty Publications

© 2015 American Society for Nutrition. The realization that low-glycemic index diets were formulated using resistant starch led to more than a decade of research on the health effects of resistant starch. Determination of the metabolizable energy of the resistant starch product allowed for the performance of isocaloric studies. Fermentation of resistant starch in rodent studies results in what appears to be a healthier gut, demonstrated by increased amounts of short-chain fatty acids, an apparent positive change in the microbiota, and increased gene expression for gene products involved in normal healthy proliferation and apoptosis of potential cancer cells. Additionally, consumption …

Role Of Resistant Starch In Improving Gut Health, Adiposity, And Insulin Resistance, Michael J. Keenan, June Zhou, Maren Hegsted, Christine Pelkman, Holiday A. Durham, Diana B. Coulon, Roy J. Martin

Faculty Publications

© 2015 American Society for Nutrition. The realization that low-glycemic index diets were formulated using resistant starch led to more than a decade of research on the health effects of resistant starch. Determination of the metabolizable energy of the resistant starch product allowed for the performance of isocaloric studies. Fermentation of resistant starch in rodent studies results in what appears to be a healthier gut, demonstrated by increased amounts of short-chain fatty acids, an apparent positive change in the microbiota, and increased gene expression for gene products involved in normal healthy proliferation and apoptosis of potential cancer cells. Additionally, consumption …

Prowashonupana Barley Dietary Fibre Reduces Body Fat And Increases Insulin Sensitivity In Caenorhabditis Elegans Model, Chenfei Gao, Michael L. King, Zachary L. Fitzpatrick, Wenqian Wei, Jason F. King, Mingming Wang, Frank L. Greenway, John W. Finley, Jeffrey H. Burton, William D. Johnson, Michael J. Keenan, Frederick M. Enright, Roy J. Martin, Jolene Zheng

Faculty Publications

© 2015 Elsevier Ltd. Prowashonupana barley (PWB) is high in β-glucan with moderate content of resistant starch. PWB reduced intestinal fat deposition (IFD) in wild type Caenorhabditis elegans (C. elegans, N2), and in sir-2.1 or daf-16 null mutants, and sustained a surrogate marker of lifespan, pharyngeal pumping rate (PPR), in N2, sir-2.1, daf-16, or daf-16/daf-2 mutants. Hyperglycaemia (2% glucose) reversed or reduced the PWB effect on IFD in N2 or daf-16/daf-2 mutants with a sustained PPR. mRNA expression of cpt-1, cpt-2, ckr-1, and gcy-8 were dose-dependently reduced in N2 or daf-16 mutants, elevated in daf-16/daf-2 mutants with reduction in cpt-1, …

Comparative Gut Microbiota Of 59 Neotropical Bird Species, Sarah M. Hird, César Sánchez, Bryan C. Carstens, Robb T. Brumfield

Faculty Publications

© 2015 Hird, Sánchez, Carstens and Brumfield. The gut microbiota of vertebrates are essential to host health. Most non-model vertebrates, however, lack even a basic description of natural gut microbiota biodiversity. Here, we sampled 116 intestines from 59 Neotropical bird species and used the V6 region of the 16S rRNA molecule as a microbial fingerprint (average coverage per bird ~80,000 reads). A core microbiota of Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria was identified, as well as several gut-associated genera. We tested 18 categorical variables associated with each bird for significant correlation to the gut microbiota; host taxonomic categories were most frequently …

The Codex Alimentarius Commission, Corporate Influence, And International Trade: A Perspective On Fda's Global Role, Sam F. Halabi

Faculty Publications

Section 305 of the FDA Food Safety Modernization Act specifically calls for FDA to develop recommendations on whether and how to harmonize requirements under the Codex Alimentarius Commission (“Codex”), an international organization charged with developing food standards, guidelines, codes of practice and “other recommendations to ensure fair practices in food trade and protect[ion of] the health of consumers.” FDA’s International Food Safety Capacity-Building Plan is largely supportive and deferential to Codex, concluding that “the use of Codex standards helps assure a safe global food supply.” To be sure, Codex’s stated mission and policies should create and facilitate adoption of universal …

Blueberries Improve Glucose Tolerance Without Altering Body Composition In Obese Postmenopausal Mice, Carrie M. Elks, Jennifer D. Terrebonne, Donald K. Ingram, Jacqueline M. Stephens

Faculty Publications

© 2015 The Obesity Society. Objective Metabolic syndrome (MetS) risk increases significantly during menopause and remains elevated postmenopause. Several botanicals, including blueberries (BB), have been shown to delay MetS progression, but few studies have been conducted in postmenopausal animal models. Here, the effects of BB supplementation on obese postmenopausal mice using a chemically induced menopause model were examined. Methods After induction of menopause, mice were fed a high-fat diet or the same diet supplemented with 4% BB powder for 12 weeks. Body weight and body composition were measured, and mice were subjected to glucose and insulin tolerance tests. Serum triglycerides …

Isothiocyanate-Rich Moringa Oleifera Extract Reduces Weight Gain, Insulin Resistance, And Hepatic Gluconeogenesis In Mice, Patricio Rojas-Silva, Tugba Boyunegmez Tumer, Peter Kuhn, Allison J. Richard, Shawna Wicks, Jacqueline M. Stephens, Zhong Wang, Randy Mynatt, William Cefalu, Ilya Raskin

Faculty Publications

© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Scope: Moringa oleifera (moringa) is tropical plant traditionally used as an antidiabetic food. It produces structurally unique and chemically stable moringa isothiocyanates (MICs) that were evaluated for their therapeutic use in vivo. Methods and results: C57BL/6L mice fed very high fat diet (VHFD) supplemented with 5% moringa concentrate (MC, delivering 66 mg/kg/d of MICs) accumulated fat mass, had improved glucose tolerance and insulin signaling, and did not develop fatty liver disease compared to VHFD-fed mice. MC-fed group also had reduced plasma insulin, leptin, resistin, cholesterol, IL-1β, TNFα, and lower hepatic glucose-6-phosphatase …

Diosgenin, 4-Hydroxyisoleucine, And Fiber From Fenugreek: Mechanisms Of Actions And Potential Effects On Metabolic Syndrome, Scott Fuller, Jacqueline M. Stephens

Faculty Publications

© 2015 American Society for Nutrition. Metabolic syndrome and its complications continue to rise in prevalence and show no signs of abating in the immediate future. Therefore, the search for effective treatments is a high priority in biomedical research. Products derived from botanicals have a time-honored history of use in the treatment of metabolic diseases including type 2 diabetes. Trigonella foenum-graecum, commonly known as fenugreek, is an annual herbaceous plant that has been a staple of traditional herbal medicine in many cultures. Although fenugreek has been studied in both clinical and basic research settings, questions remain about its efficacy and …

Fat In Flames: Influence Of Cytokines And Pattern Recognition Receptors On Adipocyte Lipolysis, Ryan W. Grant, Jacqueline M. Stephens

Faculty Publications

© 2015 the American Physiological Society. Adipose tissue has the largest capacity to store energy in the body and provides energy through the release of free fatty acids during times of energy need. Different types of immune cells are recruited to adipose tissue under various physiological conditions, indicating that these cells contribute to the regulation of adipose tissue. One major pathway influenced by a number of immune cells is the release of free fatty acids through lipolysis during both physiological (e.g., cold stress) and pathophysiological processes (e.g., obesity, type 2 diabetes). Adipose tissue expansion during obesity leads to immune cell …

Seasonal Changes In Soil Organic Matter After A Decade Of Nutrient Addition In A Lowland Tropical Forest, Benjamin L. Turner, Joseph B. Yavitt, Kyle E. Harms, Milton N. Garcia, S. Joseph Wright

Faculty Publications

© 2015, US Government. Soil organic matter is an important pool of carbon and nutrients in tropical forests. The majority of this pool is assumed to be relatively stable and to turn over slowly over decades to centuries, although changes in nutrient status can influence soil organic matter on shorter timescales. We measured carbon, nitrogen, and phosphorus concentrations in soil organic matter and leaf litter over an annual cycle in a long-term nutrient addition experiment in lowland tropical rain forest in the Republic of Panama. Total soil carbon was not affected by a decade of factorial combinations of nitrogen, phosphorus, …

Cumulative Systolic Bp And Changes In Urine Albuminto-Creatinine Ratios In Nondiabetic Participants Of The Multi-Ethnic Study Of Atherosclerosis, Paul Zemaitis, Kiang Liu, David R. Jacobs, Mary Cushman, Ramon Durazo-Arvizu, David Shoham, Walter Palmas, Richard Cooper, Holly Kramer

Faculty Publications

Background and objectives Cumulative exposure to elevated systolic BP (cumSBP) may affect progression of urine albumin excretion in the absence of diabetes. The objective of this study was to examine the association between cumSBP exposure and progression of spot urine albumin-to-creatinine ratio (UACR) in a multi-ethnic cohort of adults without diabetes. Design, setting, participants,&measurements The analysis included 3789 participants without severely increased urine albumin excretion or diabetes in the Multi-Ethnic Study of Atherosclerosis, a cohort of 6814 adults aged 45– 84 years. UACR was measured at baseline and approximately 1.6, 3.1, and 9.4 years after the baseline examination. cumSBP was …

A Resistant-Starch Enriched Yogurt: Fermentability, Sensory Characteristics, And A Pilot Study In Children, Frank Greenway, Jolene Zheng, Kayanush Aryana, Nikhil Dhurandhar, Richard Tulley, John Finley, Michael Keenan, Roy Martin, Christine Pelkman, Douglas Olson

Faculty Publications

The rising prevalence of obesity and the vulnerability of the pediatric age group have highlighted the critical need for a careful consideration of effective, safe, remedial and preventive dietary interventions. Amylose starch (RS2) from high-amylose maize (HAM) ferments in the gut and affects body weight. One hundred and ten children, of 7-8 (n=91) or 13-14 (n=19) years of age scored the sensory qualities of a yogurt supplemented with either HAM-RS2 or an amylopectin starch. The amylopectin starch yogurt was preferred to the HAM-RS2-enriched yogurt by 7-8 year old panelists (P<0.0001). Appearance, taste, and sandiness scores given by 13- to 14-year-old panelists were more favorable for the amylopectin starch yogurt than for HAM-RS2-enriched yogurt (P<0.05). HAM-RS2 supplementation resulted in acceptable (≥6 on a 1-9 scale) sensory and hedonic ratings of the yogurt in 74% of subjects. Four children consumed a HAM-RS2-enriched yogurt for four weeks to test its fermentability in a clinical trial. Three adolescents, but not the single pre-pubertal child, had reduced stool pH (P=0.1) and increased stool short-chain fatty acids (SCFAs) (P<0.05) including increased fecal acetate (P=0.02), and butyrate (P=0.089) from resistant starch (RS) fermentation and isobutyrate (P=0.01) from protein fermentation post-treatment suggesting a favorable change to the gut microbiota. HAM-RS2 was not modified by pasteurization of the yogurt, and may be a palatable way to increase fiber intake and stimulate colonic fermentation in adolescents. Future studies are planned to determine the concentration of HAM-RS2 that offers the optimal safe and effective strategy to prevent excessive fat gain in children.

Consumer Health Reference Interview: Ideas For Public Librarians, Lili Luo

Faculty Publications

This two-stage study seeks to help public librarians become better prepared, and more confident and competent, when answering medical/health questions. At the first stage, eleven barriers that public librarians often encounter in the consumer health reference interview were identified through a comprehensive literature review, and at the second stage, input from professional consumer health librarians on how to overcome the barriers was gathered via a qualitative survey. Findings of the study provide concrete and practical strategies that will help public librarians more successfully conduct the reference interview to assist library users in their health information-seeking journey.

Measuring Client Experiences Of Motivational Interviewing During A Lifestyle Intervention, Michael B. Madson, Richard S. Mohn, Julie A. Schumacher, Alicia S. Landry

Faculty Publications

The Client Evaluation of Motivational Interviewing was used to assess motivational interviewing experiences in a predominantly female, African American sample from the Southeastern United States who received motivational interviewing-based feedback during a multicomponent lifestyle intervention. Motivational interviewing was experienced differently than a primarily White, male, Northeastern mental health sample.

Longstanding Agency Interpretations, Anita S. Krishnakumar

Faculty Publications

How much deference — or what kind — should courts give to longstanding agency interpretations of statutes? Surprisingly, courts and scholars lack a coherent answer to this question. Legal scholars long have assumed that longstanding agency statutory interpretations are treated with heightened deference on judicial review, and federal courts sometimes have made statements suggesting that this is the case. But in practice, federal court review of longstanding agency interpretations — at both the Court of Appeals and the U.S. Supreme Court levels — turns out to be surprisingly erratic. Reviewing courts sometimes note the longevity of an agency’s statutory interpretation …

Spiritual Pathways To Healing And Recovery: An Intensive Single-N Study Of An Eating Disorder Patient, Kawika Allen, Troy Lea, P. Scott Richards, Peter W. Sanders, Jason A. Mcbride

Faculty Publications

This article presents an in-depth single case study of 8 sessions of spiritually integrated psychotherapy with a 20-year-old woman recovering from an Eating Disorder. The inclusion and utility of session-to-session outcome data as well as systematic follow up data in conjunction with in-depth qualitative interviews are shown. Dr. H and client’s perspectives are highlighted over the course of treatment. Clinical areas of focus were extracted from the the qualitative interviews and the therapeutic process is described. Single-case study statistical analyses were conducted to highlight clinical gains and maintenance.

Agricultural Biotechnology: Drawing On International Law To Promote Progress, J. Janewa Oseitutu

Faculty Publications

In Bowman v. Monsanto, the Supreme Court declined to apply the principle of exhaustion to limit the patentee’s ability to control the reproduction of self-replicating inventions. This decision was justified from a patent law perspective on the basis that patent holder has a right to prevent others from making the invention. But what happens when we take other perspectives into account? For instance, a farmer might have human rights or other rights that may need to be balanced against the patentee’s right. Since globalized intellectual property standards were established through international agreements and much of the resistance to intellectual property …

Toward A Synthesis Of Hr Competency Models: The Common Hr "Food Groups", Dave Ulrich, Wayne Brockbank, Mike Ulrich, David Kryscynski

Faculty Publications

As businesses face unprecedented rates of volatility, uncertainty, complexity, and ambiguity (VUCA), business leaders increasingly recognize the importance of talent (workforce, competencies, skills, abilities), leadership (senior leader and leadership team), and organization (workplace, capability, culture, processes, systems) as sources of competitiveness. Competitors can readily copy access to capital, strategic intent, and operational efficiency but they have a more difficult time copying talent, leadership, and organizational practices. As a result of this increased visibility, HR issues have received increased attention in the C-suite.

Weed And Water Law: Regulating Legal Marijuana, Ryan Stoa

Faculty Publications

Marijuana is nearing the end of its prohibition in the United States. Arguably the country’s largest cash crop, marijuana is already legal for recreational use in Colorado, Washington, Oregon, Alaska, and Washington DC. Between now and election day 2016, an additional 14 states may place marijuana legalization initiatives on their ballots. In addition, 23 states and Washington DC have legalized medical marijuana, with up to seven states pending legislation. The era of marijuana prohibition is rapidly coming to a close.

At the same time, traditional doctrines of water law are struggling to cope with the modern realities of water scarcity. …

Association Between Toxocariasis And Cognitive Function In Young To Middle-Aged Adults, Bruce L. Brown, Lance D. Erickson, Shawn D. Gale, Andrew Berrett, Dawson W. Hedges

Faculty Publications

The ascarid nematodes Toxocara canis (Werner, 1782) and Toxocara cati (Schrank, 1788) may infect humans resulting in toxocariasis. A prior study associated species of Toxocara Stiles, 1905 with cognitive deficits in children. To determine if a similar association between toxocariasis and cognition exists in adults, we analysed a large dataset from the United States’ Center for Disease Control’s National Health and Nutrition Examination Survey. We used linear-regression and multivariate models to examine the association between toxocariasis as assessed by the presence of anti-Toxocara IgG antibodies and three measures of cognitive function – simple reaction time (SRT), symbol-digit substitution (SDS) and …

Interaction Between Helicobacter Pylori And Latent Toxoplasmosis And Demographic Variables On Cognitive Function In Young To Middle-Aged Adults, Bruce L. Brown, Shawn D. Gale, Lance D. Erickson, Dawson W. Hedges

Faculty Publications

Helicobacter pylori and latent toxoplasmosis are widespread diseases that have been associated with cognitive deficits and Alzheimer’s disease. We sought to determine whether interactions between Helicobacter pylori and latent toxoplasmosis, age, race-ethnicity, educational attainment, economic status, and general health predict cognitive function in young and middle-aged adults. To do so, we used multivariable regression and multivariate models to analyze data obtained from the United States’ National Health and Nutrition Examination Survey from the Centers for Disease Control and Prevention, which can be weighted to represent the US population. In this sample, we found that 31.6 percent of women and 36.2 …

Symposium On The Challenges Of Electronic Evidence, Daniel J. Capra, Sidney A. Fitzwater, Peter Pitegoff, Jeffrey S. Sutton, Paul Grimm, John Haried, Richard W. Vorder Bruegge, Jeffrey Bellin, Paul Scechtman, Deirdre M. Smith, Shira A. Scheindlin, David Shonka, Daniel Gelb, Andrew Goldsmith, George Paul, Paul Lippe

Faculty Publications

No abstract provided.

Subsampling Reduces Sorting Effort For Waterfowl Foods In Salt-Marsh Core Samples, Mark C. Livolsi, Kevin M. Ringelman, Christopher K. Williams

Faculty Publications

Waterfowl researchers often use soil core samples to estimate food availability in foraging habitats, and these estimates are needed for bioenergetic models of carrying capacity. However, core sampling is frequently a time-and resource-intensive process, and some researchers have suggested that subsampling may be a valuable way to reduce processing time. We evaluated whether 10% and 25% by mass subsampling are appropriate techniques for reducing core-sorting effort while maintaining precision for samples taken in six separate habitat types along the Delaware bayshore. We found no significant difference between biomass found in 100% sorted cores and estimated biomass obtained by 10% and …