Digital Commons Network^™

A Biologic Argument For Gay Essentialism-Determinism: Implications For Equal Protection And Substantive Due Process, E. Gary Spitko

Faculty Publications

Classifications, like race, that historically have been used to saddle certain people with disabilities on the basis of a characteristic that otherwise would be irrelevant to an individual's ability to contribute to society are inherently suspect as more likely to have been the product of irrational prejudice and, thus, are deserving of heightened equal protection scrutiny. Thus, in adjudicating whether a sexual orientation classification is deserving of heightened equal protection scrutiny, a court should ask only whether gay people have suffered a history of discrimination and whether their sexual orientation says anything about their ability to contribute to society.

The …

The Effects Of Malnutrition On Child Mortality In Developing Countries, D.L. Pelletier, Edward A. Frongillo Jr., D.G. Schroeder, J.P. Habicht

Faculty Publications

No abstract provided.

Country/Region Reports -- United States Of America, Linda A. Malone

Faculty Publications

No abstract provided.

Biological And Genetic Characterization Of A Hemagglutinating Coronavirus Isolated From A Diarrhoeic Child, X. M. Zhang, W. Herbst, K. G. Kousoulas, J. Storz

Faculty Publications

The coronavirus strain HECV‐4408 was isolated from diarrhea fluid of a 6‐year‐old child with acute diarrhea and propagated in human rectal tumor (HRT‐18) cells. Electron microscopy revealed coronavirus particles in the diarrhea fluid sample and the infected HRT‐18 cell cultures. This virus possessed hemagglutinating and acetylesterase activities and caused cytopathic effects in HRT‐18 cells but not in MDBK, GBK and FE cells. One of four S‐specific monoclonal antibodies reacted in Western blots with HECV‐4408, BCV‐L9 and BCV‐LY138 but not with HCV‐OC43, and two reacted with BCV‐L9 but not with HECV‐4408, BCV‐LY138 and HCV‐OC43. One S‐specific and two N‐specific monoclonal antibodies …

The Human Remains From Carter-Ranch Pueblo,Arizona: Health In Isolation, Marie E. Danforth, Della Collins Cook, Stanley G. Knick Iii

Faculty Publications

Health patterns in the Pueblo III (A.D. 1100-1225) population from Carter Ranch Pueblo were investigated in skeletal remains from 34 individuals. Childhood health disruptions were assessed using stature, linear enamel hypoplasias, and Harris lines. Periostitis, arthritis, anemia, trauma, and dental pathology were also observed. Although the low juvenile representation is probably an effect of age-biased mortuary practices, results suggest a healthy population compared to larger southwestern sites. Trauma levels at the site are quite high, possibly indicating burial practices differentiated on the ability to work or other health criteria. Additionally, a number of genetic anomalies are present, suggesting an isolated …

Aids And Athletics, Matthew J. Mitten

Faculty Publications

No abstract provided.

Truncation Of The Carboxy-Terminal 28 Amino Acids Of Glycoprotein B Specified By Herpes Simplex Virus Type 1 Mutant Amb1511-7 Causes Extensive Cell Fusion, A. Baghian, L. Huang, S. Newman, S. Jayachandra, K. G. Kousoulas

Faculty Publications

Three amber mutations were introduced proximal to the syn3 locus of the herpes simplex virus type 1 glycoprotein B (gB) gene specifying gB derivatives lacking the carboxy-terminal 28, 49, or 64 amino acids. A complementation system that utilized gBs expressed in COS cells to complement gB-null virus KΔT was established. The 49- or 64-amino-acid-truncated gBs failed to complement gB-null virus KΔT, while the 28-amino-acid-truncated gB complemented KΔT efficiently. Mutant herpes simplex virus type 1 KOS (amb1511-7) specifying the 28-amino-acid-truncated gB fused Vero cells extensively.

Relationship Between Vegetation And Soil Formation In A Rapidly Submerging Coastal Marsh, J. A. Nyman, R. D. Delaune, H. H. Roberts, W. H. Patrick

Faculty Publications

In a Terrebonne Basin (Louisiana) marsh, Cs dating indicated that vertical accretion (0.98cm yr ) was extremely rapid relative to other marshes, but insufficient to counter submergence (1.38cm yr ). Mineral and organic matter accumulation were similar to that in other marshes. This marsh is threatened by a positive feedback loop of plant flooding stress and inadequate vertical accretion. Inadequate plant growth limits vertical accretion, which further increases flooding and decreases plant production. Thus plant production partly determines the degree of submergence some coastal and estuarine marshes will tolerate. -from Authors 137 -1 -1

Dna Diversity Among Clinical Isolates Of Helicobacter Pylori Detected By Pcr-Based Rapd Fingerprinting, Natalia Akopyanz, Nickolai O. Bukanov, T. Ulf Westblom, Stephen Kresovich, Douglas E. Berg

Faculty Publications

The RAPD (or AP-PCR) DNA fingerprinting method was used to distinguish among clinical isolates of Helicobacter pylori, a bacterium whose long term carriage is associated with gastritis, peptic ulcers and gastric carcinomas. This method uses arbitrarily chosen oligonucleotides to prime DNA sythesis from genomic sites to which they are fortuitously matched, or almost matched. Most 10-nt primers with > 60% G + C yielded strain-specific arrays of up to 15 prominent fragments, as did most longer ( > 17-nt) primers, whereas most 10-nt primers with 50% G +C did not. Each of 64 independent H.pylori isolates, 60 of which were from …

Beyond Guardianship Reform: A Reevaluation Of Autonomy And Beneficence For A System Of Principled Decision-Making In Long Term Care, Alison Patrucco Barnes

Faculty Publications

No abstract provided.

The Hemagglutinin/Esterase Gene Of Human Coronavirus Strain Oc43: Phylogenetic Relationships To Bovine And Murine Coronaviruses And Influenza C Virus, Xuming Zhang, Konstantin G. Kousoulas, Johannes Storz

Faculty Publications

The complete nucleotide sequences of the hemagglutinin/esterase (HE) genes of human coronavirus (HCV) strain OC43 and bovine respiratory coronavirus (BRCV) strain G95 were determined from single-stranded cDNA fragments generated by reverse transcription of virus-specific mRNAs and amplified by polymerase chain reaction. An open reading frame of 1272 nucleotides was identified as the putative HE gene by homology to the bovine coronavirus HE gene. This open reading frame encodes a protein of 424 amino acids with an estimated molecular weight of 47.7 kDa. Ten potential N-linked glycosylation sites were predicted in the HE protein of HCV-OC43 while nine of them were …

Two-Step Polymerase Chain Reactions And Restriction Endonuclease Analyses Detect And Differentiate Ompa Dna Of Chlamydia Spp., B. Kaltenboeck, K. G. Kousoulas, J. Storz

Faculty Publications

Specific and sensitive amplification of major outer membrane protein (MOMP) gene (ompA) DNA sequences of Chlamydia species with various MOMP genotypes was achieved by a two-step polymerase chain reaction (PCR). Degenerate, inosine-containing oligonucleotide primers homologous to the 5' and 3' ends of the translated regions of all chlamydial MOMP genes were used in a PCR to amplify a DNA fragment of approximately 1,120 bp. A portion of this DNA fragment was amplified in a second genus-specific reaction that yielded a DNA fragment of approximately 930 bp. A pair of degenerate oligonucleotide primers homologous to internal sequences of the primary DNA …

Involvement Of Interleukin 2 Receptors In Conceptus-Derived Suppression Of T And B Cell Proliferation In Horses, T. L. Roth, K. L. White, D. L. Thompson, S. Rahmanian, D. W. Horohov

Faculty Publications

The mechanism by which a horse conceptus-derived immunosuppressive factor (HCS) of M(r) >100 000 inhibits lymphocyte proliferation was investigated. The factor was obtained from the culture supernatants of 20-day-old horse conceptuses; activity, identified by reduced uptake of [3H]thymidine by mitogen-stimulated lymphocytes, was greatest (P0.05). Data from a fluorescence-activated cell sorter indicated that supplementation with HCS reduced the number of T cells in phytohaemagglutinin-stimulated cultures and suppressed proliferation of T and B cells in pokeweed-mitogen-stimulated cultures compared with controls. Cell proliferation was greater (P0.05) from that of control cells. The addition of stimulated equine lymphocyte supernatant to cultures supplemented with HCS …

Detection And Strain Differentiation Of Chlamydia Psittaci Mediated By A Two-Step Polymerase Chain Reaction, B. Kaltenboeck, K. G. Kousoulas, J. Storz

Faculty Publications

Specific and sensitive amplification of major outer membrane protein (MOMP) gene DNA sequences of Chlamydia psittaci was achieved in a two-step polymerase chain reaction. First, oligonucleotide primers specific for 5' and 3' nontranslated regulatory regions of the MOMP gene were used in a polymerase chain reaction to amplify a DNA fragment of approximately 1,400 bp. A portion of this DNA fragment was amplified in a second reaction using a degenerate oligonucleotide primer specific for a DNA sequence contained within the 1,400-bp DNA fragment and one of the first-step primers. This method detected 10 cognate chlamydial genomes. C. psittaci MOMP genes …

Dietary Protein Deficiency And Mycobacterium Bovis Bcg Affect Interleukin-2 Activity In Experimental Pulmonary Tuberculosis, David N. Mcmurray, C. L. Mintzer, R. A. Bartow, Rebecca D. Parr

Faculty Publications

Inbred strain 2 guinea pigs were vaccinated with Mycobacterium bovis BCG or were left unvaccinated. They were maintained for 6 weeks on defined, isocaloric diets containing either 30% (control animals) or 10% (animals receiving low protein) ovalbumin as the sole protein source. Animals were challenged by the respiratory route with a low dose of virulent M. tuberculosis H37Rv and killed 4 weeks later. Protein-malnourished animals were not protected by previous vaccination with BCG. Lymphocytes isolated from various tissues were tested in vitro for proliferative responses to mitogen (concanavalin A) and antigen (purified protein derivative [PPD]), production of interleukin-2 (IL-2), and …

A Subset Of Type-Specific Epitopes Map In The Amino Terminus Of Herpes Simplex Virus Type 1 Glycoprotein B, K. Kousoulas, M. Arsenakis, L. Pereira

Faculty Publications

No abstract provided.

A Major Neutralizing Domain Maps Within The Carboxyl-Terminal Half Of The Cleaved Cytomegalovirus B Glycoprotein, T. Banks, B. Huo, K. Kousoulas, R. Spaete, C. Pachl, L. Pereira

Faculty Publications

No abstract provided.

The Various Human Rights In Healthcare, Alison Barnes, Michael Mcchrystal

Faculty Publications

No abstract provided.

Purification, Partial Characterization, And Seroreactivity Of A Genuswide 60-Kilodalton Legionella Protein Antigen, Chou-Pong Pau, B. B. Plikaytis, G. M. Carlone, I. M. Warner

Faculty Publications

A genuswide protein antigen extracted from Legionella pneumophila serogroup 1 (strain Philadelphia 1) cells was enriched by differential pelleting and ammonium sulfate precipitation and subsequently purified with a combination of high-performance size-exclusion and ion-exchange chromatography. The protein has an apparent molecular weight of 650,000 before and 63,000 after urea (5 M) treatment, as determined by size-exclusion chromatography. These proteins resolved to a single band of 60,000 after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The urea-treated protein had an isoelectric point of 5.8. This purified 60-kilodalton protein reacted with a convalescent-phase serum sample from a patient with legionellosis and rabbit immune sera …

Religion And The State (Introduction), Gene R. Nichol

Faculty Publications

No abstract provided.

Effects Of Water Quality And Water Quantity On Nutritional Status: Findings From A South Indian Community, James R. Hébert

Faculty Publications

Quantitative assessments of the relative effects on health of various aspects of water supply are virtually absent from the literature. Despite the lack of information, resources are being allocated throughout the developing world, for projects related to water and sanitation. The present study was designed specifically to overcome many of the methodological problems that other researchers have faced. Data were collected concerning the nutritional status of 627 children in three urban communities in South India. Information was also collected on water quality, water quantity, household sanitation, socioeconomic conditions, and housing. A statistical technique is presented that allows for controlling potential …

Comparison Of Haitian Children In A Nutrition Intervention Programme With Children In The Haitian National Nutrition Survey, G. G. Berggren, James R. Hébert, C. M. Waternaux

Faculty Publications

Weight-for-height and height-for-age data were compared for preschool-age Haitian children enrolled in a community health and nutrition intervention programme and children measured in the Haiti national nutrition survey of 1978. Cross-sections of the longitudinal data of the intervention programme corresponding to the season when the national survey was conducted (May to September) were chosen for the three years of available programme data (1969, 1970, 1971). Significantly less stunting was found in children in the 1970 and 1971 intervention group than in the children covered by the national survey. Tests of trend also showed that the height (or length) status of …

Cytokine Production By Blue Tongue Virus-Infected Fetal Sheep Cells, F. M. Enright, B. I. Osburn

Faculty Publications

The migration inhibition of guinea pig peritoneal macrophages by a factor(s) from media obtained from blue tongue virus-infected monolayer cultures was studied. Medium from blue tongue virus-infected sheep fetal cell cultures inhibited migration of guinea pig macrophages from agarose droplets. Medium from control cultures and stock virus did not inhibit macrophage migration. Medium containing migration inhibiting factor(s) in vitro induced an inflammatory reaction in the skin of a newborn sheep. The inflammatory reaction was observed 20 h after intradermal inoculation. The skin reaction consisted of infiltrates of mononuclear leukocytes in the superficial dermis. Control medium and stock virus caused no …

Surrogate Management Of The Property Of The Aged, George J. Alexander

Faculty Publications

No abstract provided.

Lord Bacon, Part Iii : His Character, And Writings, Lucian Minor

Faculty Publications

No abstract provided.