Digital Commons Network^™

Anthropology & Open Access: An Interview With Jason Baird Jackson, Ryan B. Anderson

Faculty Publications

During the last few weeks I had the chance to conduct an email based interview with Jason Baird Jackson about Open Access (OA), academic publishing, and anthropology...

Ingestion Of 10 Grams Of Whey Protein Prior To A Single Bout Of Resistance Exercise Does Not Augment Akt/Mtor Pathway Signaling Compared To Carbohydrate, Geoffrey M. Hudson

Faculty Publications

Background: This study examined the effects of a whey protein supplement in conjunction with an acute bout of lower body resistance exercise, in recreationally-active males, on serum insulin and insulin like growth factor 1 (IGF-1) and Akt/mTOR signaling markers indicative of muscle protein synthesis: insulin receptor substrate 1 (IRS-1), AKT, mammalian target of rapamycin (mTOR), p70S6 kinase (p70S6K) and 4E-binding protein 1 (4E-BP1).

Methods: In a randomized, double-blind, cross-over design, 10 males ingested 1 week apart, either 10 g of whey protein (5.25 g EAAs) or carbohydrate (maltodextrose), 30 min prior to a lower-body resistance exercise bout. The …

Functional Dissection Of The Glucose Signaling Pathways That Regulate The Yeast Glucose Transporter Gene (Hxt) Repressor Rgt1, David J. Jouandot Ii, Adhiraj Roy, Jeong-Ho Kim

Faculty Publications

The yeast Rgt1 repressor is a bifunctional protein that acts as a transcriptional repressor and activator. Under glucose-limited conditions, Rgt1 induces transcriptional repression by forming a repressive complex with its corepressors Mth1 and Std1. Here, we show that Rgt1 is converted from a transcriptional repressor into an activator under high glucose conditions and this occurs through two independent but consecutive events mediated by two glucose signaling pathways: (1) disruption of the repressive complex by the Rgt2/Snf3 pathway; (2) phosphorylation of Rgt1 by the cAMP-dependent protein kinase (cAMP-PKA) pathway. Rgt1 is phosphorylated by PKA at four serine residues within its amino-terminal …

Elimination Of The Nlrp3-Asc Inflammasome Protects Against Chronic Obesity-Induced Pancreatic Damage, Yun Hee Youm, Ayinuer Adijiang, Bolormaa Vandanmagsar, David Burk, Anthony Ravussin, Vishwa Deep Dixit

Faculty Publications

Clinical evidence that the blockade of IL-1β in type-2 diabetic patients improves glycemia is indicative of an autoinflammatory mechanism that may trigger adiposity-driven pancreatic damage. IL-1β is a key contributor to the obesity-induced inflammation and subsequent insulin resistance, pancreatic β-cell dysfunction, and the onset of type 2 diabetes. Our previous studies demonstrated that the ceramides activate the Nod-like receptor family, pyrin domain containing 3 (Nlrp3) inflammasome to cause the generation of mature IL-1β and ablation of the Nlrp3 inflammasome in diet-induced obesity improves insulin signaling. However, it remains unclear whether the post-translational processing of active IL-1β in pancreas is regulated …

Genome-Wide Association Study Identifies Loci Influencing Concentrations Of Liver Enzymes In Plasma, J. C. Chambers, W. Zhang, J. Sehmi, X. Li, M. N. Wass, P. Van Der Harst, H. Holm, S. Sanna, M. Kavousi, S. E. Baumeister, L. J. Coin, G. Deng, C. Gieger, N. L. Heard-Costa, J. J. Hottenga, B. Kühnel, V. Kumar, V. Lagou, L. Liang, J. Luan, P. M. Vidal, I. Mateo Leach, P. F. O'Reilly, J. F. Peden, N. Rahmioglu, P. Soininen, E. K. Speliotes, X. Yuan, G. Thorleifsson, B. Z. Alizadeh, L. D. Atwood, I. B. Borecki, M. J. Brown

Faculty Publications

Concentrations of liver enzymes in plasma are widely used as indicators of liver disease. We carried out a genome-wide association study in 61,089 individuals, identifying 42 loci associated with concentrations of liver enzymes in plasma, of which 32 are new associations (P = 10-8 to P = 10-190). We used functional genomic approaches including metabonomic profiling and gene expression analyses to identify probable candidate genes at these regions. We identified 69 candidate genes, including genes involved in biliary transport (ATP8B1 and ABCB11), glucose, carbohydrate and lipid metabolism (FADS1, FADS2, GCKR, JMJD1C, HNF1A, MLXIPL, PNPLA3, PPP1R3B, SLC2A2 and TRIB1), glycoprotein biosynthesis …

The Efficacy Of Detecting Variants With Small Effects On The Affymetrix 6.0 Platform Using Pooled Dna, Charleston W.K. Chiang, Zofia K.Z. Gajdos, Joshua M. Korn, Johannah L. Butler, Rachel Hackett, Candace Guiducci, Thutrang T. Nguyen, Rainford Wilks, Terrence Forrester, Katherine D. Henderson, Loic Le Marchand, Brian E. Henderson, Christopher A. Haiman, Richard S. Cooper, Helen N. Lyon, Xiaofeng Zhu, Colin A. Mckenzie, Mark R. Palmert, Joel N. Hirschhorn

Faculty Publications

Genome-wide genotyping of a cohort using pools rather than individual samples has long been proposed as a cost-saving alternative for performing genome-wide association (GWA) studies. However, successful disease gene mapping using pooled genotyping has thus far been limited to detecting common variants with large effect sizes, which tend not to exist for many complex common diseases or traits. Therefore, for DNA pooling to be a viable strategy for conducting GWA studies, it is important to determine whether commonly used genome-wide SNP array platforms such as the Affymetrix 6.0 array can reliably detect common variants of small effect sizes using pooled …

Trace Metals In Porewater Of Surface Sediments And Their Bioavailability In Jiaozhou Bay, Qingdao, China, Siyuan Ye, Edward A. Laws, Xigui Ding, Hongming Yuan, Guangming Zhao, Jin Wang

Faculty Publications

Samples of porewater and the edible tissue of mottled clams, Ruditapes variegatus, were collected simultaneously at each of 12 stations from Jiaozhou Bay near Qingdao, China, in June of 2003. Chemical analysis focused on trace metals and major elements. Porewater concentrations of Cd, Cr, Pb, and Zn were distributed in a bimodal fashion among the 12 stations, with mean concentrations at six of the stations being 8-32 times mean values at the other six stations. The concentrations of the same metals in clams were remarkably similar among stations, the coefficients of variation being only 12-37%. Calculations performed with the computer …

The Nuclear Pore Complex Mediates Binding Of The Mig1 Repressor To Target Promoters, Nayan J. Sarma, Thomas D. Buford, Terry Haley, Kellie Barbara-Haley, George M. Santangelo, Kristine A. Willis

Faculty Publications

All eukaryotic cells alter their transcriptional program in response to the sugar glucose. In Saccharomyces cerevisiae, the best-studied downstream effector of this response is the glucose-regulated repressor Mig1. We show here that nuclear pore complexes also contribute to glucose-regulated gene expression. NPCs participate in glucose-responsive repression by physically interacting with Mig1 and mediating its function independently of nucleocytoplasmic transport. Surprisingly, despite its abundant presence in the nucleus of glucose-grown nup120Δ or nup133Δ cells, Mig1 has lost its ability to interact with target promoters. The glucose repression defect in the absence of these nuclear pore components therefore appears …

Science Diplomacy With Swissnex China: A Swiss Nation Brand Initiative, Flavia Schlegel, Olivier Jacot, Marc Fetscherin

Faculty Publications

Switzerland has a long tradition of expertise in the fields of science, research and education, with “science diplomacy” (SD) now assuming an important role for innovation economies. Past experience shows that international scientific cooperation can have valuable outcomes for the involved countries and complements traditional foreign policy and diplomacy. In Switzerland, a worldwide network of science and technology outposts under the auspices of the Swiss State Secretariat for Education and Research (SER) in cooperation with the Federal Department of Foreign Affairs (FDFA) was established in 2000. The very swissnex network around the world defines one of those established science diplomacy …

Rethinking Extraordinary Circumstances, Scott Dodson

Faculty Publications

This short essay for Northwestern University Law Review's Colloquy seeks to rationalize the "extraordinary circumstances" doctrine of Rue 60(b)(6) of the Federal Rules of Civil Procedure. The usual rule is that a movant for Rule 60(b)(6) relief must show extraordinary circumstances for that relief. Under the Ackermann rule (so named after the Supreme Court decision that spawned it), courts have held that any extraordinary circumstances cannot have been caused by the movant's own litigation conduct. I argue that the Ackermann rule, at its broadest, would be unjust to those litigants most in need of Rule 60(b)(6) relief and would overserve …

Social And Clinical Predictors Of Prostate Cancer Treatment Decisions Among Men In South Carolina, Sara E. Wagner, Bettina F. Drake, Keith Elder, James R. Hébert

Faculty Publications

OBJECTIVE:

To assess social and clinical influences of prostate cancer treatment decisions among white and black men in the Midlands of South Carolina.

METHODS:

We linked data collected on treatment decision making in men diagnosed with prostate cancer from 1996 through 2002 with clinical and sociodemographic factors collected routinely by the South Carolina Central Cancer Registry (SCCCR). Unconditional logistic regression was used to assess social and clinical influences on treatment decision.

RESULTS:

A total of 435 men were evaluated. Men of both races who chose surgery (versus radiation) were more likely to be influenced by their physician and by family/friends. …

Patients’ Reports About Medical Doctors’ Inquiries On Their Mental Health: Do Generational Status, Ethnicity And Mental Health/Substance Use Disorders Matter?, Van M. Ta Park, P. Holck, T. Chen, N. Zane

Faculty Publications

Immigrants are less likely than others to use mental health (MH) services. Physicians' limited time often precludes inquiry about MH. This study investigated the influence of generational status, ethnicity, and mental/substance use disorders on physicians' inquiries about Asian American (AA) MH. Data from the National Latino and Asian American Study were analyzed (n=1,853). The outcome was past year physician's inquiry regarding MH. Results revealed that AA with U.S.-born parents had significantly greater odds compared to AA born outside the U.S. to report that their doctors inquired about their MH (OR=218, 95% CI: 1.28, 3.73). Past year mental/substance use disorder increased …

Adipose Tissue Endocannabinoid System Gene Expression: Depot Differences And Effects Of Diet And Exercise, Tongjian You, Beth L. Disanzo, Xuewen Wang, Rongze Yang, Dawei Gong

Faculty Publications

Background: Alterations of endocannabinoid system in adipose tissue play an important role in lipid regulation and metabolic dysfunction associated with obesity. The purpose of this study was to determine whether gene expression levels of cannabinoid type 1 receptor (CB1) and fatty acid amide hydrolase (FAAH) are different in subcutaneous abdominal and gluteal adipose tissue, and whether hypocaloric diet and aerobic exercise influence subcutaneous adipose tissue CB1 and FAAH gene expression in obese women.

Methods: Thirty overweight or obese, middle-aged women (BMI = 34.3 ± 0.8 kg/m², age = 59 ± 1 years) underwent one of three 20-week weight …

Modeling Nitrogen Loading In A Small Watershed In Southwest China Using A Dndc Model With Hydrological Enhancements, Jia Deng, Zaixing Zhou, Bo Zhu, Xunhua Zheng, Changsheng Li, X. Wang, Z. Jian

Faculty Publications

The degradation of water quality has been observed worldwide, and inputs of nitrogen (N), along with other nutrients, play a key role in the process of contamination. The quantification of N loading from non-point sources at a watershed scale has long been a challenge. Process-based models have been developed to address this problem. Because N loading from non-point sources result from interactions between biogeochemical and hydrological processes, a model framework must include both types of processes if it is to be useful. This paper reports the results of a study in which we integrated two fundamental hydrologic features, the SCS …

A Draft Genome Of Yersinia Pestis From Victims Of The Black Death, Kirsten I. Bos, Verena J. Schuenemann, G. Brian Golding, Hernán A. Burbano, Nicholas Waglechner, Brian K. Coombes, Joseph B. Mcphee, Sharon Dewitte, Matthias Meyer, Sarah Schmedes, James Wood, David J. D. Earn, D. Ann Herring, Peter Bauer, Hendrik N. Poinar, Johannes Krause

Faculty Publications

Technological advances in DNA recovery and sequencing have drastically expanded the scope of genetic analyses of ancient specimens to the extent that full genomic investigations are now feasible and are quickly becoming standard1. This trend has important implications for infectious disease research because genomic data from ancient microbes may help to elucidate mechanisms of pathogen evolution and adaptation for emerging and re-emerging infections. Here we report a reconstructed ancient genome of Yersinia pestis at 30-fold average coverage from Black Death victims securely dated to episodes of pestilence-associated mortality in London, England, 1348–1350. Genetic architecture and phylogenetic analysis indicate …

Prescribing Exercise In Primary Care: Ten Practical Steps On How To Do It, Karim M. Khan, Richard Weller, Steven N. Blair

Faculty Publications

No abstract provided.

Influence Of Substrate Modification And C-Terminal Truncation On The Active Site Structure Of Substrate-Bound Heme Oxygenase From Neisseriae Meningitidis. A 1H Nmr Study, Dungeng Peng, James D. Satterlee, Li Hua Ma, Jerry L. Dallas, Kevin M. Smith, Xuhong Zhang, Michihiko Sato, Gerd N. La Mar

Faculty Publications

Heme oxygenase (HO), from the pathogenic bacterium N. meningitidis(NmHO), which secures host iron, shares many properties with mammalian HOs but also exhibits some key differences. The crystal structure appears more compact, and the crystal-undetected C-terminus interacts with substrate in solution. The unique nature of substrate-protein, specifically pyrrole-I/II-helix-2, peripheral interactions in NmHO are probed by 2D 1H NMR to reveal unique structural features controlling substrate orientation. The thermodynamics of substrate orientational isomerism are mapped for substrates with individual vinyl → methyl → hydrogen substitutions and with enzyme C-terminal deletions. NmHO exhibits significantly stronger orientational preference, reflecting much stronger and selective pyrrole-I/II …

Finding Shelter, Hyveth Williams

Faculty Publications

No abstract provided.

Oxidative Stress Response To Short Duration Bout Of Submaximal Aerobic Exercise In Healthy Young Adults, Keith M. Diaz, Deborah L. Feairheller, Kathleen M. Sturgeon, Sheara T. Williamson, Michael D. Brown

Faculty Publications

The purpose of this study was to investigate the oxidative stress response to a short duration bout of submaximal exercise in a cohort of healthy young adults. 15 apparently healthy college age males and females completed a modified Bruce-protocol treadmill test to 75–80% of their heart rate reserve. Blood samples collected immediately before (pre-exercise), immediately after, 30, 60 and 120 minutes post-exercise were assayed for total antioxidant capacity (TAC), superoxide disumutase (SOD), thiobarbituric acid-reactive substances (TBARS), and protein carbonyls (PC). SOD activity was significantly increased from pre-exercise levels at 30 minutes (77%), 60 minutes (33%), and 120 minutes (37%) post-exercise. …

Why Not Consider A Spherical Protein? Implications Of Backbone Hydrogen Bonding For Protein Structure And Function, Michal Brylinski, Mu Gao, Jeffrey Skolnick

Faculty Publications

The intrinsic ability of protein structures to exhibit the geometric features required for molecular function in the absence of evolution is examined in the context of three systems: the reference set of real, single domain protein structures, a library of computationally generated, compact homopolypeptides, artificial structures with protein-like secondary structural elements, and quasi-spherical random proteins packed at the same density as proteins but lacking backbone secondary structure and hydrogen bonding. Without any evolutionary selection, the library of artificial structures has similar backbone hydrogen bonding, global shape, surface to volume ratio and statistically significant structural matches to real protein global structures. …

Genetic Variants In Novel Pathways Influence Blood Pressure And Cardiovascular Disease Risk, Georg B. Ehret, Patricia B. Munroe, Kenneth M. Rice, Murielle Bochud, Andrew D. Johnson, Daniel I. Chasman, Albert V. Smith, Martin D. Tobin, Germaine C. Verwoert, Shih Jen Hwang, Vasyl Pihur, Peter Vollenweider, Paul F. O'Reilly, Najaf Amin, Jennifer L. Bragg-Gresham, Alexander Teumer, Nicole L. Glazer, Lenore Launer, Jing Hua Zhao, Yurii Aulchenko, Simon Heath, Siim Sober, Afshin Parsa, Jian'an Luan, Pankaj Arora, Abbas Dehghan, Feng Zhang, Gavin Lucas, Andrew A. Hicks, Anne U. Jackson, John F. Peden, Toshiko Tanaka, Sarah H. Wild

Faculty Publications

Blood pressure is a heritable trait influenced by several biological pathways and responsive to environmental stimuli. Over one billion people worldwide have hypertension (≥140mmg Hg systolic blood pressure ≥90mmg Hg diastolic blood pressure). Even small increments in blood pressure are associated with an increased risk of cardiovascular events. This genome-wide association study of systolic and diastolic blood pressure, which used a multi-stage design in 200,000 individuals of European descent, identified sixteen novel loci: six of these loci contain genes previously known or suspected to regulate blood pressure (GUCY1A3 GUCY1B3, NPR3 C5orf23, ADM, FURIN FES, GOSR2, GNAS EDN3); the other ten …

Rumi Functions As Both A Protein O-Glucosyltransferase And A Protein O-Xylosyltransferase, Hideyuki Takeuchi, Rodrigo C. Fernańdez-Valdivia, Devin S. Caswell, Aleksandra Nita-Lazar, Nadia A. Rana, Thomas P. Garner, Thomas K. Weldeghiorghis, Megan A. Macnaughtan, Hamed Jafar-Nejad, Robert S. Haltiwanger

Faculty Publications

Mutations in rumi result in a temperature-sensitive loss of Notch signaling in Drosophila. Drosophila Rumi is a soluble, endoplasmic reticulum-retained protein with a CAP10 domain that functions as a protein O-glucosyltransferase. In human and mouse genomes, three potential Rumi homologues exist: one with a high degree of identity to Drosophila Rumi (52%), and two others with lower degrees of identity but including a CAP10 domain (KDELC1 and KDELC2). Here we show that both mouse and human Rumi, but not KDELC1 or KDELC2, catalyze transfer of glucose from UDPglucose to an EGF repeat from human factor VII. Similarly, human Rumi, but …

A Comparison Of The Primary And Secondary Wood Products Sectors In Louisiana, Richard P. Vlosky

Faculty Publications

As part of an ongoing research program, the Louisiana forest products industry is surveyed every five years to identify salient issues, challenges, and opportunities. In this paper, we compare the results from two studies conducted on the primary and secondary sectors in Louisiana. In the past, we produced two papers based on research results, one for the primary and one for the secondary sector. However, this paper compares the answers to questions asked in both surveys. Louisiana's forests represent an important resource for the state, both in terms of income to landowners and as inputs to the forest products industry. …

Shaping A Lfe Of Significance For Retirement By R. Jack Hansen And Jerry P. Haas, E Michael Brady Phd

Faculty Publications

The article reviews the book Shaping a Life of Significance for Retirement, by R. Jack Hansen and Jerry P. Haas.

Diagnosing Liability : The Legal History Of Posttraumatic Stress Disorder, Deirdre M. Smith

Faculty Publications

This Article examines the origins of the unique relationship between the psychiatric diagnosis Posttraumatic Stress Disorder (PTSD) and the law and considers the implications of that relationship for contemporary uses of the diagnosis in legal settings. PTSD stands apart from all other diagnoses in psychiatry 's standard classification system, the Diagnostic and Statistical Manual of Mental Disorders (DSM} , and is the focus of significant controversy within psychiatry, because its diagnostic criteria require a determination of causation. By diagnosing a person with PTSD, a clinician necessarily assigns responsibility to a specific event or agent for causing the person 's symptoms, …

Lochner V. New York (1905) And Kennedy V. Louisiana (2008): Judicial Reliance On Adversary Argument, Douglas E. Abrams

Faculty Publications

Chief Justice William H. Rehnquist called Lochner v. New York (1905) “one of the most ill-starred decisions that [the Supreme Court ever rendered.” The Justices’ deliberations preceding the 5-4 decision demonstrate the courts’ reliance on advocacy in the adversary system of civil and criminal justice. The stark imbalance between the state’s “incredibly sketchy” brief and Joseph Lochner’s sterling submission may have determined Lochner’s outcome, and thus may have changed the course of constitutional history, by leading two Justices to join the majority on the central question of whether New York’s maximum-hours law for bakery workers was a reasonable public health …

Dietary-Resistant Starch Improves Maternal Glycemic Control In Goto-Kakizaki Rat, Li Shen, Michael J. Keenan, Anne Raggio, Cathy Williams, Roy J. Martin

Faculty Publications

Scope: Dietary prebiotics show potential in anti-diabetes. Dietary resistant starch (RS) has a favorable impact on gut hormone profiles, including glucagon-like peptide-1 (GLP-1) consistently released, a potent anti-diabetic incretin. Also RS reduced body fat and improved glucose tolerance in rats and mice. In the current project, we hypothesize that dietary-resistant starch can improve insulin sensitivity and pancreatic β cell mass in a type 2 diabetic rat model. Altered gut fermentation and microbiota are the initial mechanisms, and enhancement in serum GLP-1 is the secondary mechanism. Methods and results: In this study, GK rats were fed an RS diet with 30% …

Dietary-Resistant Starch Improves Maternal Glycemic Control In Goto-Kakizaki Rat, Li Shen, Michael J. Keenan, Anne Raggio, Cathy Williams, Roy J. Martin

Faculty Publications

Scope: Dietary prebiotics show potential in anti-diabetes. Dietary resistant starch (RS) has a favorable impact on gut hormone profiles, including glucagon-like peptide-1 (GLP-1) consistently released, a potent anti-diabetic incretin. Also RS reduced body fat and improved glucose tolerance in rats and mice. In the current project, we hypothesize that dietary-resistant starch can improve insulin sensitivity and pancreatic β cell mass in a type 2 diabetic rat model. Altered gut fermentation and microbiota are the initial mechanisms, and enhancement in serum GLP-1 is the secondary mechanism. Methods and results: In this study, GK rats were fed an RS diet with 30% …

Arbitration Ambush In A Policy Polemic, Amy J. Schmitz

Faculty Publications

Arbitration has been demonized in the media and consumer protection debates, often without empirical support or consideration of its attributes. This has led to renewed efforts to pass the Arbitration Fairness Action, which would bar enforcement of pre-dispute arbitration clauses in consumer, employment, and civil rights contexts. It also inspired Dodd-Frank’s preclusion of arbitration clauses in mortgage contracts, along with the Consumer Financial Protection Bureau’s charge to prohibit or limit enforcement of pre-dispute arbitration agreements in consumer financial products and services contracts. Some of this negativity toward arbitration is warranted, especially in the wake of the United Supreme Court’s recent …

Problem Profiles Of At-Risk Youth In Two Service Programs: A Multigroup Exploratory Latent Class Analysis, Richard Dembo, Rhissa Briones-Robinson, Rocío Aracelis Ungaro, Lora M. Karas, Laura M. Gulledge, Paul Greenbaum, James Schmeidler, Ken C. Winters, Steven Belenko

Faculty Publications

Baseline data collected in two brief intervention projects (BI-Court and Truancy Project) were used to assess similarities and differences in subgroups of at-risk youth. Classifications of these subgroups were based on their psychosocial characteristics (e. g., substance use). Multigroup latent class analysis identified two BI-Court subgroups of youth and three truant subgroups. These classes can be viewed as differing along two dimensions, substance use involvement and emotional and behavioral issues. Equality tests of means across the latent classes for BI-Court and Truancy Project youths found significant differences that were consistent with their problem group classification. These findings highlight the importance …