Digital Commons Network^™

The 9th Conference On Metal Toxicity And Carcinogenesis: The Conference Overview, James T.F. Wise, Lei Wang, Zhuo Zhang, Xianglin Shi

Faculty Publications

Heavy metals, such as arsenic, chromium, cadmium, nickel, mercury, and uranium are known to cause many human diseases and health complications after occupational or environmental exposure. Consequently, metals are environmental health concerns. This manuscript is an overview of the 9th Conference on Metal Toxicity and Carcinogenesis held in October 2016 in Lexington, Kentucky. Since 2000, this biennial meeting brings together experts in the field to discuss current and prospective research in an effort to advance research pertaining to metal toxicity and carcinogenesis. In this review we summarize the major topics discussed and provide insight regarding current research in the field …

Early Rearing History Influences Oxytocin Receptor Epigenetic Regulation In Rhesus Macaques, Maggie Baker, Stephen G. Lindell, Carlos A. Driscoll, Zhifeng Zhou, Qiaoping Yuan, Melanie L. Schwandt, Isaac Miller-Crews, Elizabeth A. Simpson, Annika Paukner, Pier Francesco Ferrari, Ravi Kumar Sindhu, Muslima Razaqya, Wolfgang H. Sommer, Juan F. Lopez, Robert C. Thompson, David Goldman, Markus Heilig, J. Dee Higley, Stephen J. Suomi, Christina S. Barr

Faculty Publications

Adaptations to stress can occur through epigenetic processes and may be a conduit for informing offspring of environmental challenge. We employed ChIP-sequencing for H3K4me3 to examine effects of early maternal deprivation (peer-rearing, PR) in archived rhesus macaque hippocampal samples (male, n = 13). Focusing on genes with roles in stress response and behavior, we assessed the effects of rearing on H3K4me3 binding by ANOVA. We found decreased H3K4me3 binding at genes critical to behavioral stress response, the most robust being the oxytocin receptor gene OXTR, for which we observed a corresponding decrease in RNA expression. Based on this finding, we …

Spatiotemporal Patterns Of Childhood Asthma Hospitalization And Utilization In Memphis Metropolitan Area From 2005 To 2015, Tonny J. Oyana, Pradeep Podila, Jagila Minso Wesley, Slawo Lomnicki, Stephania Cormier

Faculty Publications

© 2017 Taylor & Francis Group, LLC. Objective: To identify the key risk factors and explain the spatiotemporal patterns of childhood asthma in the Memphis metropolitan area (MMA) over an 11-year period (2005–2015). We hypothesize that in the MMA region this burden is more prevalent among urban children living south, downtown, and north of Memphis than in other areas. Methods: We used a large-scale longitudinal electronic health record database from an integrated healthcare system, Geographic information systems (GIS), and statistical and space-time models to study the spatiotemporal distributions of childhood asthma at census tract level. Results: We found statistically significant …

Reproductive, Maternal, Neonatal And Child Health In Conflict: A Case Study On Syria Using Countdown Indicators, Jocelyn Dejong, Hala Ghattas Ph.D., Hyam Bashour, Rima Mourtada, Chaza Akik, Amelia Reese-Masterson

Faculty Publications

Introduction Women and children account for a disproportionate morbidity burden among conflictaffected populations, and yet they are not included in global accountability frameworks for women's and children's health. We use Countdown to 2015 (Millennium Development Goals) health indicators to provide an up-to-date review and analysis of the best available data on Syrian refugees in Jordan, Lebanon and Turkey and internally displaced within Syria and explore data challenges in this conflict setting.

Methods We searched Medline, PubMed, Scopus, Popline and Index Medicus for WHO Eastern Mediterranean Region Office and relevant development/humanitarian databases in all languages from January 2011 until December 2015. …

Elevated Expression Of Hypoxanthine Guanine Phosphoribosyltransferase Within Malignant Tissue, Michelle H. Townsend, Abigail M. Felsted, Zachary E. Ence, Stephen R. Piccolo, Richard A. Robison, Kim L. O'Neill

Faculty Publications

Hypoxanthine Guanine Phosphoribosyltransferase (HPRT) is a housekeeping enzyme involved in the purine synthesis of guanine and inosine in the salvage pathway. While other salvage pathway enzymes, such as TK1, have been established as biomarkers for both cancer cell proliferation and cancer development, little research been done to evaluate whether HPRT has the same potential as a cancer biomarker. We designed this study to determine if HPRT has value as an identifier of malignancy within the most common types of cancer. We utilized histological samples from lung, colon, prostate, and breast cancer with additional normal tissue to evaluate whether there was …

Invisible Outsiders: Developing A Working Alliance With Appalachian Clients, Jake Protivnak, Cassandra Pusateri, Matthew Paylo, Kyoung Choi

Faculty Publications

Appalachian clients are often ‘invisible’ within the majority culture and possess characteristics unique to the region that must be considered within the counseling relationship (Tang & Russ, 2007).  Individuals in Appalachia have higher incidences of certain mental health disorders and substance use as compared to the national average (Appalachian Regional Commission [ARC], 2008).  Although the need for mental health services is evident, limited research exists to inform mental health professionals how to deliver culturally competent interventions to build a working alliance with Appalachian clients.  The authors will discuss a framework for mental health professionals to develop a strong working alliance …

Salinization And Arsenic Contamination Of Surface Water In Southwest Bangladesh, John C. Ayers, Gregory George, David Fry, Laura Benneyworth, Carol Wilson, Leslie Auerbach, Kushal Roy, Md Rezaul Karim, Farjana Akter, Steven Goodbred

Faculty Publications

To identify the causes of salinization and arsenic contamination of surface water on an embanked island (i.e., polder) in the tidal delta plain of SW Bangladesh we collected and analyzed water samples in the dry (May) and wet (October) seasons in 2012-2013. Samples were collected from rice paddies (wet season), saltwater ponds used for brine shrimp aquaculture (dry season), freshwater ponds and tidal channels (both wet and dry season), and rainwater collectors. Continuous measurements of salinity from March 2012 to February 2013 show that tidal channel water increases from ~0.15 ppt in the wet season up to ~20 ppt in …

Parasite Biodiversity Faces Extinction And Redistribution In A Changing Climate, Colin J. Carlson, Kevin R. Burgio, Eric R. Dougherty, Anna J. Phillips, Veronica M. Bueno, Christopher F. Clements, Giovanni Castaldo, Tad Dallas, Carrie A. Cizauskas, Graeme S. Cumming, Jorge Doña, Nyeema C. Harris, Roger Jovani, Sergey Mironov, Oliver C. Muellerklein, Heather C. Proctor, Wayne M. Getz

Faculty Publications

Climate change is a well-documented driver of both wildlife extinction and disease emergence, but the negative impacts of climate change on parasite diversity are undocumented. We compiled the most comprehensive spatially explicit data set available for parasites, projected range shifts in a changing climate, and estimated extinction rates for eight major parasite clades. On the basis of 53,133 occurrences capturing the geographic ranges of 457 parasite species, conservative model projections suggest that 5 to 10% of these species are committed to extinction by 2070 from climate-driven habitat loss alone. We find no evidence that parasites with zoonotic potential have a …

Alcohol-Induced Risky Sexual Behavior Among Socially Anxious Drinkers, Meredith A. Terlecki, Ashley A. Richter, Clayton Neighbors, Chelsie M. Young, Julia D. Buckner

Faculty Publications

Heavy college drinking is associated with risky sexual behavior. It is therefore important to identify groups that are especially vulnerable to alcohol-influenced sexual risk (e.g., unplanned/unexpected sex). Undergraduates with elevated social anxiety represent one such vulnerable group given that social anxiety is associated with fear of intimacy and heavier drinking in intimate situations and situations with expected negative affect. Drinking to cope with negative affect induced by fear of intimacy might render socially anxious undergraduates vulnerable to risky sexual behavior, yet no known studies have examined this relationship. The current study tested whether social anxiety was related to alcohol-related sexual …

Methods For Real-Time Prediction Of The Mode Of Travel Using Smartphone-Based Gps And Accelerometer Data, Bryan D. Martin, Vittorio Addona, Julian Wolfson, Gediminas Adomavicius, Yingling Fan

Faculty Publications

We propose and compare combinations of several methods for classifying transportation activity data from smartphone GPS and accelerometer sensors. We have two main objectives. First, we aim to classify our data as accurately as possible. Second, we aim to reduce the dimensionality of the data as much as possible in order to reduce the computational burden of the classification. We combine dimension reduction and classification algorithms and compare them with a metric that balances accuracy and dimensionality. In doing so, we develop a classification algorithm that accurately classifies five different modes of transportation (i.e., walking, biking, car, bus and rail) …

The California Linkages Program: Doorway To Housing Support For Child Welfare‐Involved Parents, Amy D'Andrade, James Simon, Danna Fabella, Lolita Castillo, Cesar Mejia, David Shuster

Faculty Publications

Housing instability can complicate parents’ efforts to provide for their children. Child welfare service agencies have had difficulty adequately serving parents’ housing needs due to limited and constrained funding streams. This article integrates the voices of four important stakeholders to illuminate how an innovative model of service system coordination called Linkages addresses housing needs for child welfare‐involved parents eligible for public assistance. Facilitated by Linkages, these parents can receive supportive housing services through programs affiliated with the California public assistance program CalWORKs. Personal narratives reflecting the diverse perspectives of stakeholders in the Linkages collaboration—the statewide program director, a child welfare …

The Role Of Efferent Reduction Of Cochlear Compression In The Detection Of Tones In Noise, Shaum Bhagat, Anusha Yellamsetty

Faculty Publications

Stimulation of medial olivocochlear (MOC) efferent neurons reduces basilar membrane (BM) sensitivity and increases the slope of BM input-output (I/O) functions in animal models. Decreased compression of I/O functions associated with activation of MOC efferent neurons may assist in extending the neural response to the tone above that of noise, leading to an improvement in masked thresholds. To evaluate this hypothesis, the distortion-product otoacoustic emission (DPOAE) I/O function, a proxy measure of BM compression, was examined in conditions with presentation of contralateral noise. DPOAE I/O functions were measured at f2 frequencies of 1000 and 2000 Hz in 16 normal-hearing adults. …

Parasite Biodiversity Faces Extinction And Redistribution In A Changing Climate, Colin J. Carlson, Kevin R. Burgio, Eric R. Dougherty, Anna J. Phillips, Veronica M. Bueno, Christopher F. Clements, Giovanni Castaldo, Tad A. Dallas, Carrie A. Cizauskas, Graeme S. Cumming, Jorge Doña, Nyeema C. Harris, Roger Jovani, Sergey Mironov, Oliver C. Muellerklein, Heather C. Proctor, Wayne M. Getz

Faculty Publications

Copyright © 2017 The Authors, some rights reserved. Climate change is a well-documented driver of both wildlife extinction and disease emergence, but the negative impacts of climate change on parasite diversity are undocumented. We compiled the most comprehensive spatially explicit data set available for parasites, projected range shifts in a changing climate, and estimated extinction rates for eight major parasite clades. On the basis of 53, 133 occurrences capturing the geographic ranges of 457 parasite species, conservative model projections suggest that 5 to 10% of these species are committed to extinction by 2070 from climate-driven habitat loss alone. We find …

Characteristics Of Ovarian Cancer Detection By A Near-Infrared Fluorescent Probe Activated By Human Nad(P)H: Quinone Oxidoreductase Isozyme 1 (Hnqo1), Yuko Nakamura, Zhenhua Shen, Toshiko Harada, Tadanobu Nagaya, Kazuhide Sato, Shuhei Okuyama, Fusa Ogata, Peter L. Choyke, Robin L. Mccarley, Hisataka Kobayashi

Faculty Publications

© Nakamura et al. Near-infrared (NIR) fluorescent probes are ideal for in vivo imaging, because they offer deeper tissue penetration by the light and lower background autofluorescence than fluorophores that emit in the visible range. Q3STCy is a newly synthesized, NIR light-emitting probe that is activated by an enzyme commonly overexpressed in tumor cells, human nicotinamide adenine dinucleotide (phosphate): quinone oxidoreductase isozyme 1, known as hNQO1 or DT-diaphorase. The purpose of this study is to compare the sensitivity of detecting peritoneal ovarian cancer metastasis (POCM) with Q3STCy and gGlu-HMRG, a green fluorescent probe, upon their surface application. In vitro uptake …

Evolution Of Alu Subfamily Structure In The Saimiri Lineage Of New World Monkeys, Mark Batzer, Jasmine N. Baker, Jerilyn A. Walker, John A. Vanchiere, Kacie R. Philippe, Corey P. St. Romain, Paulina Gonzalez-Quiroga, Michael W. Denham, Jackson R. Mierl, Miriam K. Konkel

Faculty Publications

Squirrelmonkeys,Saimiri,arecommonlyfoundinzoologicalparksandusedinbiomedicalresearch.S.boliviensisisthemostcommon species for research; however, there is little information about genome evolution within this primate lineage. Here, we reconstruct the Alu element sequence amplification and evolution in the genus Saimiri at the time of divergence within the family Cebidae lineage. Alu elements are the most successful SINE (Short Interspersed Element) in primates. Here, we report 46 Saimiri lineage specificAlusubfamilies.RetrotranspositionactivityinvolvedsubfamiliesrelatedtoAluS,AluTa10,andAluTa15.Manysubfamiliesare simultaneously active within the Saimiri lineage, a finding which supports the stealth model of Alu amplification. We also report a high resolution analysis of Alu subfamilies within the S. boliviensis genome [saiBol1].

Alu Insertion Polymorphisms As Evidence For Population Structure In Baboons, Mark Batzer, Cody J. Steely, Jerilyn A. Walker, Vallmer E. Jordan, Thomas O. Beckstrom, Cullen L. Mcdaniel, Corey P. St. Romain, Emily C. Bennett, Arianna Robichaux, Brooke N. Clement, Muthuswamy Raveendran, Kim C. Worley, Jane Phillips-Conroy, Clifford J. Jolly, Jeff Rogers, Miriam K. Konkel

Faculty Publications

Male dispersal from the natal group at or near maturity is a feature of most baboon (Papio) species. It potentially has profound effects upon population structure and evolutionary processes, but dispersal, especially for unusually long distances, is not readily documented by direct field observation. In this pilot study, we investigate the possibility of retrieving baboon population structure in yellow (Papio cynocephalus) and kinda (Papio kindae) baboons from the distribution of variation in a genome-wide set of 494 Alu insertion polymorphisms, made available via the recently completed Baboon Genome Analysis Consortium. Alu insertion variation in a mixed …

Short Communication: Lack Of Support For Socially Connected Hiv-1 Transmission Among Young Adult Black Men Who Have Sex With Men, Kayo Fujimoto, Lyndon M. Coghill, Christopher A. Weier, Lu Yu Hwang, Ju Yeong Kim, John A. Schneider, Michael L. Metzker, Jeremy M. Brown

Faculty Publications

© Copyright 2017, Mary Ann Liebert, Inc. 2017. We explore the phylogenetic relationships among HIV sequences sampled from young adult black men who have sex with men (YAB-MSM), who are connected through peer referral/social ties and who attend common venues. Using 196 viral sequences sampled from the peripheral blood mononuclear cells of 10 individuals, our preliminary phylogenetic results indicate that these socially connected YAB-MSM are infected with distantly related viruses and provide no evidence for viral transmission between network members. Our results suggest that HIV-prevention strategies that target young adult MSM should extend beyond their network members and local community.

Expanding Hot Flow In The Black Hole Binary Swift J1753.5-0127: Evidence From Optical Timing, Alexandra Veledina, Poshak Gandhi, Robert Hynes, Jari J.E. Kajava, Sergey S. Tsygankov, Michail G. Revnivtsev, Martin Durant, Juri Poutanen

Faculty Publications

We describe the evolution of optical and X-ray temporal characteristics during the outburst decline of the black hole X-ray binary SWIFT J1753.5-0127. The optical/X-ray crosscorrelation function demonstrates a single positive correlation at the outburst peak, then it has multiple dips and peaks during the decline stage, which are then replaced by the precognition dip plus peak structure in the outburst tail. Power spectral densities and phase lags show a complex evolution, revealing the presence of intrinsically connected optical and X-ray quasi-periodic oscillations. For the first time, we quantitatively explain the evolution of these timing properties during the entire outburst within …

Effect Of Dietary Salt Intake On Epithelial Na+ Channels (Enac) In Vasopressin Magnocellular Neurosecretory Neurons In The Rat Supraoptic Nucleus, Kaustubh Sharma, Masudul Haque, Richard Guidry, Yoichi Ueta, Ryoichi Teruyama

Faculty Publications

© 2017 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society Key points: A growing body of evidence suggests that epithelial Na+ channels (ENaCs) in the brain play a significant role in the regulation of blood pressure; however, the brain structures that mediate the effect are not well understood. Because vasopressin (VP) neurons play a pivotal role in coordinating neuroendocrine and autonomic responses to maintain cardiovascular homeostasis, a basic understanding of the regulation and activity of ENaC in VP neurons is of great interest. We show that high dietary salt …

Response By Bress Et Al To Letters Regarding Article, "Potential Deaths Averted And Serious Adverse Events Incurred From Adoption Of The Sprint (Systolic Blood Pressure Intervention Trial) Intensive Blood Pressure Regimen In The United States: Projections From Nhanes (National Health And Nutrition Examination Survey)", Adam P. Bress, Holly Kramer, Richard S. Cooper

Faculty Publications

No abstract provided.

Evolution Of Alu Subfamily Structure In The Saimiri Lineage Of New World Monkeys, Jasmine N. Baker, Jerilyn A. Walker, John A. Vanchiere, Kacie R. Phillippe, Corey P. St Romain, Paulina Gonzalez-Quiroga, Michael W. Denham, Jackson R. Mierl, Miriam K. Konkel, Mark A. Batzer

Faculty Publications

© The Author 2017. Squirrel monkeys, Saimiri, are commonly found in zoological parks and used in biomedical research. S. Boliviensis is the most common species for research; however, there is little information about genome evolution within this primate lineage. Here, we reconstruct the Alu element sequence amplification and evolution in the genus Saimiri at the time of divergence within the family Cebidae lineage. Alu elements are the most successful SINE (Short Interspersed Element) in primates. Here, we report 46 Saimiri lineage specific Alu subfamilies. Retrotransposition activity involved subfamilies related to AluS, AluTa10, and AluTa15. Many subfamilies are simultaneously active within …

Alu Insertion Polymorphisms As Evidence For Population Structure In Baboons, Cody J. Steely, Jerilyn A. Walker, Vallmer E. Jordan, Thomas O. Beckstrom, Cullen L. Mcdaniel, Corey P. St Romain, Emily C. Bennett, Arianna Robichaux, Brooke N. Clement, Muthuswamy Raveendran, Kim C. Worley, Jane Phillips-Conroy, Clifford J. Jolly, Jeff Rogers, Miriam K. Konkel, Mark A. Batzer, Yue Liu, Shwetha Murali, Tauras P. Vilgalys, Jerilyn A. Walker

Faculty Publications

© The Author 2017. Male dispersal from the natal group at or near maturity is a feature of most baboon (Papio) species. It potentially has profound effects upon population structure and evolutionary processes, but dispersal, especially for unusually long distances, is not readily documented by direct field observation. In this pilot study, we investigate the possibility of retrieving baboon population structure in yellow (Papio cynocephalus) and kinda (Papio kindae) baboons from the distribution of variation in a genome-wide set of 494 Alu insertion polymorphisms, made available via the recently completed Baboon Genome Analysis Consortium. Alu insertion variation in a mixed …

In-Home Firearm Access Among Us Adolescents And The Role Of Religious Subculture: Results From A Nationally Representative Study, Samuel Stroope, Joshua C. Tom

Faculty Publications

Religious participation is linked to numerous positive safety outcomes for adolescents. Scant attention, however, has been paid to associations between religious participation and safety risks among adolescents. Using data from Add Health (N = 18,449), a nationally representative school-based sample of US adolescents, this study examines the relationship between adolescents' religious affiliation and easy access to firearms at home. Regression analyses adjust for complex sampling design and compare easy firearm access at home among conservative Protestant adolescents to adolescent firearm access in other religious traditions. Conservative Protestant adolescents have a substantially greater likelihood of easy access to a gun at …

Effect Of Metabolic Status On Conceptus–Maternal Interactions On Day 19 In Dairy Cattle: Ii. Effects On The Endometrial Transcriptome, Stefan Bauersachs, Constantine A. Simintiras, Roger G. Sturmey, Stefan Krebs, Jochen Bick, Helmut Blum, Eckhard Wolf, Pat Lonergan, Niamh Forde

Faculty Publications

The aim of this study was to test the hypothesis that the metabolic stresses associated with lactation alter the ability of the endometrium to respond appropriately to the conceptus by examining endometrial gene expression on day 19 of pregnancy. Immediately after calving, primiparous Holstein cows with similar production and fertility estimated breeding values were randomly divided into two groups and either dried off (i.e. never milked) immediately or milked twice daily. Approximately 65–75 days postpartum, grade 1 blastocysts recovered from superovulated Holstein heifer donors (n = 5) were transferred (1 per recipient) into lactating (n = 11) and nonlactating (n …

Amblyomma Maculatum Secis Binding Protein 2 And Putative Selenoprotein P Are Indispensable For Pathogen Replication And Tick Fecundity, Khemraj Budachetri, Gary Crispell, Shahid Karim

Faculty Publications

Selenium, a vital trace element, is incorporated into selenoproteins to produce selenocysteine. Our previous studies have revealed an adaptive co-evolutionary process that has enabled the spotted fever-causing tick-borne pathogen Rickettsia parkeri to survive by manipulating an antioxidant defense system associated with selenium, which includes a full set of selenoproteins and other antioxidants in ticks. Here, we conducted a systemic investigation of SECIS binding protein 2 (SBP2) and putative selenoprotein P (SELENOP) by transcript silencing in adult female Gulf-coast ticks (Amblyomma maculatum). Knockdown of the SBP2 and SELENOP genes depleted the respective transcript levels of these tick selenogenes, and …

Ethanol-Induced Stress Response Of Staphylococcus Aureus, Jasmine M. Pando, Richard F. Pfeltz, Jesus A. Cuaron, Vijayaraj Nagarajan, Mukti N. Mishra, Nathaniel J. Torres, Mohamed O. Elasri, Brian J. Wilkinson, John E. Gustafson

Faculty Publications

Transcriptional profiles of 2 unrelated clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates were analyzed following 10% (v/v) ethanol challenge (15 min), which arrested growth but did not reduce viability. Ethanol-induced stress (EIS) resulted in differential gene expression of 1091 genes, 600 common to both strains, of which 291 were upregulated. With the exception of the downregulation of genes involved with osmotic stress functions, EIS resulted in the upregulation of genes that contribute to stress response networks, notably those altered by oxidative stress, protein quality control in general, and heat shock in particular. In addition, genes involved with …

A Whooping Cough Education Module For Wic Clients In Utah, Karlen E. Luthy, Alicia Anderson, Janelle L. B. Macintosh, Renea L. Beckstrand, Lacey M. Eden, Ryan Amy, Christopher I. Macintosh

Faculty Publications

Background: Clients in the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) are required to complete education modules quarterly to maintain eligibility. The purposes of this project were to: (1) create a whooping cough vaccination education module for WIC clients; (2) evaluate baseline perceptions of WIC clients on the whooping cough vaccine and disease; and (3) evaluate whooping cough knowledge following completion of the module. Problem: A decline in vaccination rates among infants and children using WIC services was reported by a local WIC program director who requested whooping cough vaccination education materials. This quality improvement project included …

Clostridium Tertium Peritonitis And Concurrent Bacteremia In A Patient With A History Of Alcoholic Cirrhosis, S. Scott Sutton, Mark Jumper, Ansal Shah, Babatunde Edun

Faculty Publications

Spontaneous bacterial peritonitis (SBP) is a recognized cause of morbidity and mortality in cirrhotic patients. Enterobacteriaceae have been isolated from the majority of peritonitis cases and the gram negative aerobe Escherichia coli is the most commonly isolated organism. Anaerobic organisms are rarely isolated because of the high oxygen tension in ascetic fluid. We report a patient with a history of alcoholic cirrhosis who developed SBP and concurrent bacteremia with the anaerobe Clostridium tertium. The patient was successfully treated with intravenous antibiotics and was discharged home on oral ciprofloxacin. This case report is unique in that it is the fourth …

Effect Of Alcohol Dose On Deliberate Self-Harm In Men And Women, Mitchell E. Berman, Jennifer R. Fanning, Casey R. Guillot, Angelika Marsic, Joshua Bullock, Michael R. Nadorff, Michael S. Mccloskey

Faculty Publications

Objective: Nonexperimental survey and field research support the notion that alcohol use may be associated with deliberate self-harm (DSH) across the spectrum of lethality, from nonsuicidal self-injury (NSSI) through suicide. Nonexperimental studies, however, provide limited information about potential causal relationships between alcohol consumption and DSH. Two previous experiments showed that a relatively high-dose of alcohol increases the likelihood of engaging in DSH in men, with DSH defined by the self-administration of a "painful" shock (the self-aggression paradigm [SAP]; Berman & Walley, 2003; McCloskey & Berman, 2003). In this study, we examined whether (a) lower doses of alcohol also elicit DSH, …

Application Of An Advanced Maximum Likelihood Estimation Restoration Method For Enhanced-Resolution And Contrast In Second-Harmonic Generation Microscopy, Mayandi Sivaguru, Mohammad M. Kabir, Manas Ranjan Gartia, David S.C. Biggs, Barghav S. Sivaguru, Vignesh A. Sivaguru, Glenn A. Fried, Gang Logan Liu, Sakthivel Sadayappan, Kimani C. Toussaint

Faculty Publications

Second-harmonic generation (SHG) microscopy has gained popularity because of its ability to perform submicron, label-free imaging of noncentrosymmetric biological structures, such as fibrillar collagen in the extracellular matrix environment of various organs with high contrast and specificity. Because SHG is a two-photon coherent scattering process, it is difficult to define a point spread function (PSF) for this modality. Hence, compared to incoherent two-photon processes like two-photon fluorescence, it is challenging to apply the various PSF-engineering methods to improve the spatial resolution to be close to the diffraction limit. Using a synthetic PSF and application of an advanced maximum likelihood estimation …