Digital Commons Network^™

Conservation Easements: A Tool For Preserving Wildlife Habitat On Private Lands, Robin M. Rotman, Sarah A. Brown, Michael A. Powell, Sonja A. Wilhelm Stanis

Faculty Publications

Conservation easements are an essential tool for conserving private lands, and they have great potential for enhancing wildlife habitat and biodiversity. Private land conservation in the United States is likely to increase in the coming years, in light of Executive Order No. 14,008, issued by President Joseph Biden on January 27, 2021, which set a goal of conserving at least 30% of U.S. lands and waters by 2030 (Executive Office of the President 2021). There is, therefore, a need to evaluate the effect of conservation easements on wildlife habitat and biodiversity and to make recommendations for further enhancing the effectiveness …

The Case Of The Missing Device Patents, Or: Why Device Patents Matter, Erika Lietzan, Kristina M. L. Acri, Evan Weidner

Faculty Publications

A company that earns premarket approval of its medical device is entitled to an extension of one patent claiming the device, to make up for some of the time it spent doing premarket research. Yet, surprisingly, a mere thirteen percent of those eligible for this extension (also known as patent term "restoration") ask for one. In contrast, most drug companies entitled to this same patent extension ask for one.

In this Article, we attribute the imbalance largely to differences between the two regulatory frameworks. In brief, because the FDA classifies and regulates devices based on what they do and how …

Solutions Still Searching For A Problem: A Call For Relevant Data To Support "Evergreening" Allegations, Erika Lietzan, Kristina M. L. Acri

Faculty Publications

For years pharmaceutical policymaking discussions have been revolving around allegations of supposed “evergreening” by pharmaceutical companies, and policymakers have considered a range of significant policy reforms — including to antitrust law and drug regulatory law — to address this purported problem. This paper evaluates empirical data offered to substantiate “evergreening” and explains that these data — though mostly accurate — do not support proposed policy changes.

The “evergreening” claim is that by securing additional patents and FDA-related exclusivities after approval of their new drugs, brand drug companies enjoy a period of exclusivity in the market that is longer than the …

Evaluation Of Fumigant And Non-Fumigant Nematicides For Management Of Rotylenchulus Reniformis On Sweetpotato, Tristan T. Watson, Caleb S. Hamm, J. Cole Gregorie

Faculty Publications

Reniform nematode (Rotylenchulus reniformis) is a major pest of sweetpotato in many production regions in Southern United States. Applying soil fumigants and non-fumigant nematicides are the primary management strategies available to growers. This study compared the relative efficacy of nematicides (1,3-dichloropropene, fluopyram, oxamyl, fluazaindolizine, aldicarb, Majestene, and fluensulfone) for management of reniform nematode on sweetpotato. Fumigating soil with 1,3-dichloropropene consistently reduced soil population densities of reniform nematode at the time of planting in both trial years (31 - 36% reduction relative to the untreated control); however, the duration of suppression varied greatly by growing season. A similar trend was observed …

Factors That Predict Weight Loss Success Differ By Diet Intervention Type, Jordan Losavio, Michael J. Keenan, Elizabeth A. Gollub, Heidi J. Silver

Faculty Publications

Background: Many types of diet intervention can achieve negative energy balance and successful weight loss in persons with obesity. However, within any dietary strategy, there is large inter-individual variation in the weight loss response. The aim of this study is to determine factors that predict weight loss success for diet interventions that vary by macronutrient and caloric composition. Methods: Participants with BMI 30.0 to 49.9 kg/m2 self-selected one of three diet intervention trials for weight loss: low carbohydrate (LOW CHO), low fat (LOW FAT), or low calorie (LOW KCAL). Multivariable regression models were developed to determine the significance of predictor …

Comparative Genomics Of The Niche-Specific Plant Pathogen Streptomyces Ipomoeae Reveal Novel Genome Content And Organization, Natasha R. Soares, José C. Huguet-Tapia, Dongli Guan, Christopher A. Clark, Kuei Ting Yang, Olivia R. Kluchka, Raju S. Thombal, Rendy Kartika, Jonathan H. Badger, Gregg S. Pettis

Faculty Publications

The sweet potato soil rot pathogen Streptomyces ipomoeae differs in disease pathology, host range, and virulence factor production from Streptomyces species that cause scab diseases on potato and other plant hosts. Nevertheless, previous phylogenomic analysis suggested S. ipomoeae and the oldest scab species, Streptomyces scabiei, are derived from a common ancestor. While genomes of scab pathogens have been described in some detail, similar knowledge of S. ipomoeae has been lacking. Here, we performed comparative genomic analyses involving both virulent and avirulent strains of S. ipomoeae, along with other plant-pathogenic and saprophytic Streptomyces spp. The txt gene cluster for the phytotoxin …

Effect Of Storage Temperature And Produce Type On The Survival Or Growth Of Listeria Monocytogenes On Peeled Rinds And Fresh-Cut Produce, Juan Moreira, Erika Mera, Vijay Singh Chhetri, Joan M. King, Thanos Gentimis, Achyut Adhikari

Faculty Publications

Whole and fresh-cut produce are minimally processed and susceptible to microbial contamination. This study evaluated the survival or growth of L. monocytogenes on peeled rinds, and fresh-cut produce at different storage temperatures. Fresh-cut fruits and vegetables, including cantaloupe, watermelon, pear, papaya, pineapple, broccoli, cauliflower, lettuce, bell pepper, and kale (25 g pieces) were spot inoculated with 4 log CFU/g of L. monocytogenes and stored at 4 or 13°C for 6 days. Cantaloupe and bell pepper rind disks (20 cm2), mimicking whole produce were inoculated with low inoculum level (4 log CFU/mL) and high inoculum level (6 log CFU/mL) and stored …

Targeting The Aspergillus Flavus P2c Gene Through Host-Induced Gene Silencing Reduces A. Flavus Infection And Aflatoxin Contamination In Transgenic Maize, Yenjit Raruang, Olanike Omolehin, Dongfang Hu, Qijian Wei, Surassawadee Promyou, Lidiya J. Parekattil, Kanniah Rajasekaran, Jeffrey W. Cary, Kan Wang, Zhi Yuan Chen

Faculty Publications

Aspergillus flavus is an opportunistic fungal pathogen that infects maize and produces aflatoxins. Using biocontrol or developing resistant cultivars to reduce aflatoxin contamination has only achieved limited success. Here, the A. flavus polygalacturonase gene (p2c) was targeted for suppression through host-induced gene silencing (HIGS) to reduce aflatoxin contamination in maize. An RNAi vector carrying a portion of the p2c gene was constructed and transformed into maize B104. Thirteen out of fifteen independent transformation events were confirmed to contain p2c. The T2 generation kernels containing the p2c transgene had less aflatoxin than those without the transgene in six out of eleven …

Biology, Genetic Diversity, And Conservation Of Wild Bees In Tree Fruit Orchards, Olivia Kline, Ngoc T. Phan, Mitzy F. Porras, Joshua Chavana, Coleman Z. Little, Lilia Stemet, Roshani S. Acharya, David J. Biddinger, Gadi V.P. Reddy, Edwin G. Rajotte, Neelendra K. Joshi

Faculty Publications

Different species of bees provide essential ecosystem services by pollinating various agricultural crops, including tree fruits. Many fruits and nuts depend on insect pollination, primarily by wild and managed bees. In different geographical regions where orchard crops are grown, fruit growers rely on wild bees in the farmscape and use orchard bees as alternative pollinators. Orchard crops such as apples, pears, plums, apricots, etc., are mass-flowering crops and attract many different bee species during their bloom period. Many bee species found in orchards emerge from overwintering as the fruit trees start flowering in spring, and the active duration of these …

Invasiveness, Biology, Ecology, And Management Of The Fall Armyworm, Spodoptera Frugiperda, Marc Kenis, Giovanni Benelli, Antonio Biondi, Paul André Calatayud, Roger Day, Nicolas Desneux, Rhett D. Harrison, Darren Kriticos, Ivan Rwomushana, Johnnie Van Den Berg, François Verheggen, Yong Jun Zhang, Lakpo Koku Agboyi, Régis Besmer Ahissou, Malick N. Ba, Julio Bernal, Adeney Freitas De Bueno, Yves Carrière, Geraldo Andrade Carvalho, Xue Xin Chen, Lizette Cicero, Hannalene Du Plessis, Regan Early, Patrick Fallet, Komi K.M. Fiaboe, Dnyaneshwar M. Firake, Georg Goergen, Astrid T. Groot, Raul N.C. Guedes, Ankita Gupta, Gao Hu, F. N. Huang, Lara R. Jaber

Faculty Publications

The fall armyworm (FAW), Spodoptera frugiperda (JE Smith, 1797), is a serious pest of several crops, particularly maize and other cereals. It has long been known as a pest in the Americas and has invaded most of Africa and parts of the Middle East, Asia, and Australia in the last six years. Its new status as an invasive species causing serious damage in many regions worldwide has highlighted the need for better understanding and has generated much research. In this article, we provide a comprehensive review of FAW covering its (i) taxonomy, biology, ecology, genomics, and microbiome, (ii) worldwide status …

Deep Brain Stimulation For Substance Use Disorder: A Systematic Review And Meta-Analysis, Nour Shaheen, Ahmed Shaheen, Can Sarica, Arun Singh, Mario Zanaty, Karim Johari, Andrew Yang, Theresa Zesiewicz, Brian Dalm, Yarema Bezchlibnyk, Andres M. Lozano, Oliver Flouty

Faculty Publications

Objective: Substance use disorder (SUD) is a significant public health issue with a high mortality rate. Deep brain stimulation (DBS) has shown promising results in treating SUD in certain cases. In this study, we conducted a meta-analysis to evaluate the efficacy of DBS in the treatment of SUD and reduction of relapse rates. Methods: We performed a thorough and methodical search of the existing scientific literature, adhering to the PRISMA guidelines, to identify 16 original studies that fulfilled our inclusion criteria. We used the evidence levels recommended by the Oxford Centre for Evidence-Based Medicine to assess bias. The R version …

Preliminary Findings From A Collaborative Storytelling Program On Life Participation And Communication Among Persons With Aphasia, Bijoyaa Mohapatra, Ranjini Mohan

Faculty Publications

Aims: The purpose of this study is to evaluate the outcomes of a remotely delivered collaborative storytelling program based on the TimeslipsTM approach in persons with aphasia (PWA). The program aims to improve social interaction, communication, and participation through a guided experience. Methods: Six PWA of mild-moderate severity and of fluent and nonfluent types participated in the program twice a week for seven weeks. They collaboratively created 28 free-form stories from staged pictures. PWA were tested pre- and post-treatment on several validated outcome measures representing each of the Living with Aphasia: Framework for Outcome Measurement (A-FROM) domains. PWA self-rated on …

Bamboo Nanocellulose/Montmorillonite Nanosheets/Polyethyleneimine Gel Adsorbent For Methylene Blue And Cu(Ii) Removal From Aqueous Solutions, Xuelun Zhang, Feng Li, Xiyu Zhao, Jiwen Cao, Shuai Liu, You Zhang, Zihui Yuan, Xiaobo Huang, Cornelis F. De Hoop, Xiaopeng Peng, Xingyan Huang

Faculty Publications

In recent years, the scarcity of pure water resources has received a lot of attention from society because of the increasing amount of pollution from industrial waste. It is very important to use low-cost adsorbents with high-adsorption performance to reduce water pollution. In this work, a gel adsorbent with a high-adsorption performance on methylene blue (MB) and Cu(II) was prepared from bamboo nanocellulose (BCNF) (derived from waste bamboo paper) and montmorillonite nanosheet (MMTNS) cross-linked by polyethyleneimine (PEI). The resulting gel adsorbent was characterized by Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (SEM), X-ray photoelectron spectroscopic (XPS), etc. …

Design, Alpha Testing, And Beta Testing Of A 3-D Printed Open-Hardware Portable Cryopreservation Device For Aquatic Species, William M. Childress, Yue Liu, Terrence R. Tiersch

Faculty Publications

Efforts in development of germplasm repositories to preserve genetic resources of aquatic species are impeded globally by a lack of standardized, inexpensive, reproducible, and portable cryopreservation technologies. The present work demonstrates a 3-D printed standardizable freezing device that can be used with nitrogen vapor shipping dewars for on-site sperm cryopreservation for aquatic species and be distributed as open-hardware. This device could hold 22 French straws (0.25-mL or 0.5-mL) and a quick-release ring design could eject straws directly into a canister inside a dewar by pressing a button after freezing. The final prototypes produced cooling rates of 1 to 68°C/min for …

Integrated Processes Turning Pepper Sauce Waste Into Valuable By-Products, Jicheng Shu, Yongqin Yin, Zhijun Liu

Faculty Publications

Background: Safe and efficient disposal of millions of tons of pepper sauce waste (PSW) can be a challenge to pepper sauce manufacturers that are concerned about creating environmental hazards from the processing (e.g., preservative salt and vinegar) and intrinsic (e.g., the pungent capsaicins) ingredients. It will be immensely beneficial to process these waste materials before they go to disposal. This work presents integrated approaches for a complete utilization of waste materials by removing and recovering valuable by-products and/or ingredients while succeeding to minimal to zero hazards. Methods: Laboratory- and pilot-scale extraction processes were used to demonstrate the recovery of intrinsic …

Acute Stressors Do Not Impair Short-Term Memory Or Attention In An Aged Mouse Model Of Amyloidosis, Giuliana M. Dimarco, Breanna N. Harris, Alena V. Savonenko, Paul L. Soto

Faculty Publications

Memory impairment in Alzheimer’s disease patients is thought to be associated with the accumulation of amyloid-beta peptides and tau proteins. However, inconsistent reports of cognitive deficits in pre-clinical studies have raised questions about the link between amyloid-beta and cognitive decline. One possible explanation may be that studies reporting memory deficits often involve behavioral assessments that entail a high stress component. In contrast, in tasks without a high stress component transgenic mice do not consistently show declines in memory. The glucocorticoid cascade hypothesis of aging and the vicious cycle of stress framework suggest that stress exacerbates dementia progression by initiating a …

Remote Assessment Of Negative Symptoms Of Schizophrenia, David G. Daniel, Alex S. Cohen, Dawn Velligan, Phillip D. Harvey, Larry Alphs, Michael Davidson, William Potter, Alan Kott, Nina Schooler, Christopher R. Brodie, Raeanne C. Moore, Pierre Lindenmeyer, Stephen R. Marder

Faculty Publications

In contrast to the validated scales for face-to-face assessment of negative symptoms, no widely accepted tools currently exist for remote monitoring of negative symptoms. Remote assessment of negative symptoms can be broadly divided into 3 categories: (1) remote administration of an existing negative-symptom scale by a clinician, in real time, using videoconference technology to communicate with the patient; (2) direct inference of negative symptoms through detection and analysis of the patient's voice, appearance, or activity by way of the patient's smartphone or other device; and (3) ecological momentary assessment, in which the patient self-reports their condition upon receipt of periodic …

Guest Editor’S Introduction: The Need For A Broader, More Inclusive Approach To Child And Adolescent Suicide Prevention, Ryan M. Hill

Faculty Publications

No abstract provided.

The Effects Of Internet-Based Cognitive Behavioral Therapy For Suicidal Ideation Or Behaviors On Depression, Anxiety, And Hopelessness In Individuals With Suicidal Ideation: Systematic Review And Meta-Analysis Of Individual Participant Data, Lasse B. Sander, Marie Beisemann, Philipp Doebler, Hannah Moon Micklitz, Ad Kerkhof, Pim Cuijpers, Philip Batterham, Alison Calear, Helen Christensen, Eva De Jaegere, Matthias Domhardt, Annette Erlangsen, Ozlem Eylem-Van Bergeijk, Ryan Hill, Charlotte Mühlmann, Marie Österle, Jeremy Pettit, Gwendolyn Portzky, Lena Steubl, Bregje Van Spijker, Joseph Tighe, Aliza Werner-Seidler, Rebekka Büscher

Faculty Publications

Background: Suicide is a global public health problem. Digital interventions are considered a low-threshold treatment option for people with suicidal ideation or behaviors. Internet-based cognitive behavioral therapy (iCBT) targeting suicidal ideation has demonstrated effectiveness in reducing suicidal ideation. However, suicidal ideation often is related to additional mental health problems, which should be addressed for optimal care. Yet, the effects of iCBT on related symptoms, such as depression, anxiety, and hopelessness, remain unclear. Objective: We aimed to analyze whether digital interventions targeting suicidal ideation had an effect on related mental health symptoms (depression, anxiety, and hopelessness). Methods: We systematically searched CENTRAL, …

A Review Of The Fundamental Concepts Of Cfd For Novice Users In Building Research And Design, Soo Jeong Jo, James R. Jones

Faculty Publications

Computational Fluid Dynamics (CFD) has been known as the most effective method for airflow analyses, but its complexity has been a barrier to dissemination in the building industry. Although, there have been continuous efforts to simplify the simulation process and develop more user-friendly CFD tools for novice users with reduced complexity, utilizing the tools without understanding the fundamental knowledge may bring about misleading results. The purpose of the present study is to help novice users understand the configuration process of building simulations using CFD by reviewing the fundamental concepts and proposing a framework for a simulation process in interaction with …

Horizon Scanning For Potentially Invasive Non-Native Marine Species To Inform Trans-Boundary Conservation Management – Example Of The Northern Gulf Of Mexico, Kathryn A. O’Shaughnessy, Lorenzo Vilizzi, Wesley Daniel, Monica E. Mcgarrity, Hanna Bauer, Leslie Hartman, Stephen Geiger, Paul Sammarco, Steve Kolian, Scott Porter, Jessica Dutton, Matthew R. Mcclure, Michael Norberg, Alex Fogg, Timothy J. Lyons, Justin Procopio, Lauren Bantista, Wayne Bennett, Mary Wicksten, David Reeves, Julie Lively, Elizabeth Robinson, Jorge Brenner, Joseph Goy, Ashley Morgan-Olvera, Anna L.E. Yunnie, Gordon H. Copp

Faculty Publications

Prevention of non-native species introductions and establishment is essential to avoid adverse impacts of invasive species in marine environments. To identify potential new invasive species and inform non-native species management options for the northern Gulf of Mexico (Alabama, Mississippi, Louisiana, Texas), 138 marine species were risk screened for current and future climate conditions using the Aquatic Species Invasiveness Screening Kit. Species were risk-ranked as low, medium, high, and very high risk based on separate (calibrated) thresholds for fishes, tunicates, and inverte-brates. In the basic screening, 15 fishes, two tunicates, and 26 invertebrates were classified as high or very high risk …

Rbp4-Gal4, A Germline Driver That Activates In Meiosis, Reveals Functions For Vcp In Spermatid Development, Tyler J. Butsch, Alyssa E. Johnson, K. Adam Bohnert

Faculty Publications

Valosin-containing protein (VCP) is a versatile and ubiquitously expressed AAA+ ATPase that regulates multiple stages of Drosophila spermatogenesis. While VCP has documented roles in mitotic spermatogonia and meiotic spermatocytes, it is also highly expressed in post-meiotic spermatids, suggesting potential late-stage developmental functions as well. However, tools to assess late-stage activities of pleiotropic spermatogenesis genes such as VCP are lacking. Available germline-specific Gal4 drivers activate in stem cells or spermatogonia; consequently, knocking down VCP using one of these drivers disrupts or blocks early germ-cell development, precluding analysis of VCP in later stages. A Gal4 driver that activates later in development, such …

Identification Of Oral Therapeutics Using An Ai Platform Against The Virus Responsible For Covid-19, Sars-Cov-2, Adam Bess, Frej Berglind, Supratik Mukhopadhyay, Michal Brylinski, Chris Alvin, Fanan Fattah, Kishor M. Wasan

Faculty Publications

Purpose: This study introduces a sophisticated computational pipeline, eVir, designed for the discovery of antiviral drugs based on their interactions within the human protein network. There is a pressing need for cost-effective therapeutics for infectious diseases (e.g., COVID-19), particularly in resource-limited countries. Therefore, our team devised an Artificial Intelligence (AI) system to explore repurposing opportunities for currently used oral therapies. The eVir system operates by identifying pharmaceutical compounds that mirror the effects of antiviral peptides (AVPs)—fragments of human proteins known to interfere with fundamental phases of the viral life cycle: entry, fusion, and replication. eVir extrapolates the probable antiviral efficacy …

Black:White Health Inequalities, Genes Versus Environment—A New Chapter?, Richard Cooper

Faculty Publications

No abstract provided.

Gene-Educational Attainment Interactions In A Multi-Population Genome-Wide Meta-Analysis Identify Novel Lipid Loci, Lisa De Las Fuentes, Karen L. Schwander, Michael R. Brown, Amy R. Bentley, Thomas W. Winkler, Yun Ju Sung, Patricia B. Munroe, Clint L. Miller, Hugo Aschard, Stella Aslibekyan, Traci M. Bartz, Lawrence F. Bielak, Jin Fang Chai, Ching Yu Cheng, Rajkumar Dorajoo, Mary F. Feitosa, Xiuqing Guo, Fernando P. Hartwig, Andrea Horimoto, Ivana Kolčić, Elise Lim, Yongmei Liu, Alisa K. Manning, Jonathan Marten, Solomon K. Musani, Raymond Noordam, Sandosh Padmanabhan, Tuomo Rankinen, Melissa A. Richard, Paul M. Ridker, Albert V. Smith, Dina Vojinovic, Alan B. Zonderman

Faculty Publications

Introduction: Educational attainment, widely used in epidemiologic studies as a surrogate for socioeconomic status, is a predictor of cardiovascular health outcomes. Methods: A two-stage genome-wide meta-analysis of low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), and triglyceride (TG) levels was performed while accounting for gene-educational attainment interactions in up to 226,315 individuals from five population groups. We considered two educational attainment variables: “Some College” (yes/no, for any education beyond high school) and “Graduated College” (yes/no, for completing a 4-year college degree). Genome-wide significant (p < 5 × 10−8) and suggestive (p < 1 × 10−6) variants were identified in Stage 1 (in up to 108,784 individuals) through genome-wide analysis, and those variants were followed up in Stage 2 studies (in up to 117,531 individuals). Results: In combined analysis of Stages 1 and 2, we identified 18 novel lipid loci (nine for LDL, seven for HDL, and two for TG) by two degree-of-freedom (2 DF) joint tests of main and interaction effects. Four loci showed significant interaction with educational attainment. Two loci were significant only in cross-population analyses. Several loci include genes with known or suggested roles in adipose (FOXP1, MBOAT4, SKP2, STIM1, STX4), brain (BRI3, FILIP1, FOXP1, LINC00290, LMTK2, MBOAT4, MYO6, SENP6, SRGAP3, STIM1, TMEM167A, TMEM30A), and liver (BRI3, FOXP1) biology, highlighting the potential importance of brain-adipose-liver communication in the regulation of lipid metabolism. An investigation of the potential druggability of genes in identified loci resulted in five gene targets shown to interact with drugs approved by the Food and Drug Administration, including genes with roles in adipose and brain tissue. Discussion: Genome-wide interaction analysis of educational attainment identified novel lipid loci not previously detected by analyses limited to main genetic effects.

Biochemical Alterations In The Follicular Fluid Of Bovine Peri-Ovulatory Follicles And Their Association With Final Oocyte Maturation, Noof Abdulrahman Alrabiah, Constantine A. Simintiras, Alexander C.O. Evans, Patrick Lonergan, Trudee Fair

Faculty Publications

Follicular fluid (FF), a product of vascular transudate and granulosa and thecal cell secretions, is the milieu that has evolved to support oocyte growth and maturation which plays a central role in oocyte quality determination. Therefore, a suboptimal FF composition may be reflected in compromised oocyte progression through maturation, fertilization, or embryo development. To date, the composition of bovine FF remains understudied. To address this, we comprehensively characterized the metabolomic constituency of bovine FF in the period during which the oocyte undergoes meiotic maturation. More specifically, FF from pre (-24 h) and peri (-2 h)-ovulatory follicles was profiled by high-throughput …

Estrus Response Patches, Timing For Artificial Insemination, And Gnrh Protocol In Split Timed Ai Beef Cattle, Danilo Demeterco, Ryon S. Walker, Luiz Ernandes Kozicki, Vitor Mohad Valle, A. K. Edwards, Jake E. Anderson, Cathleen Collet Williams

Faculty Publications

An estrous-detection patch was used to determine the optimum timing for STAI and the necessity of GnRH at STAI on a 7-day CO-Synch+CIDR protocol. Crossbred beef cows (n=216) were stratified into the following treatment groups: CTRL=TAI (n=67) at 72 h post CIDR removal, or TRT=STAI (n=149) at 72 or 84 h post CIDR removal. All females received GnRH (100 mcg) plus a CIDR on d0, PGF2α, CIDR removal, and an Estrotect estrous-detector patch on d7. At 72 h post-CIDR removal, a patch score was assigned (PS150% removed) to all females. Cows in the CTRL group were administered a second GnRH …

Evidence For The Efficacy Of Pre-Harvest Agricultural Practices In Mitigating Food-Safety Risks To Fresh Produce In North America, Naresh Devarajan, Daniel L. Weller, Matthew Jones, Aiko D. Adell, Achyut Adhikari, Ana Allende, Nicole L. Arnold, Patrick Baur, Sarah M. Beno, Donna Clements, Elissa M. Olimpi, Faith Critzer, Hyatt Green, Lisa Gorski, Angela Ferelli Gruber, Jasna Kovac, Jeffery Mcgarvey, Claire M. Murphy, Sarah I. Murphy, Nora Navarro-Gonzalez, Jeb P. Owen, Alda F.A. Pires, Nicole Richard, Sandipan Samaddar, Radomir Schmidt, Kate Scow, Nikki W. Shariat, Olivia M. Smith, Austin R. Spence, Don Stoeckel, Thao D.H. Tran, Gretchen Wall, Daniel S. Karp

Faculty Publications

Consumption of contaminated produce remains a leading cause of foodborne illness. Increasingly, growers are altering agricultural practices and farm environments to manage food-safety hazards, but these changes often result in substantial economic, social, and environmental costs. Here, we present a comprehensive evidence synthesis evaluating the efficacy of soil, non-crop vegetation, animal, landscape, and irrigation water management strategies aimed at reducing produce-safety risk in North America. We systematically summarized findings from 78 peer-reviewed papers on the effect of 21 management practices on the prevalence, abundance, or survival of four foodborne pathogens (i.e., E. coli, Salmonella spp., Listeria spp., and Campylobacter spp.), …

Optimization Of Lycopene Extraction From Tomato Pomace And Effect Of Extract On Oxidative Stability Of Peanut Oil, Dinh Thi Tran, Lan Thi Hoang Nguyen, Cuong Ngoc Nguyen, Maarten L.A.T.M. Hertog, Bart Nicolaï, David Picha

Faculty Publications

Lycopene, the pigment principally responsible for the red colour of tomatoes, is well-known for its strong antioxidant property and substantial health benefits. In this study, the optimal combination of factors for lycopene extraction from tomato pomace was identified using response surface methodology. The best practically feasible results maximising both lycopene yield and antioxidant activity of the extract were obtained by applying a ratio of ethyl acetate to tomato waste of 35:1 (v/w), at a temperature of 55°C for 100 min of extraction. The extract was then added to the peanut oil to evaluate its oxidative stability during 60 days of …

Comprehensive Meta-Analysis Of Qtl And Gene Expression Studies Identify Candidate Genes Associated With Aspergillus Flavus Resistance In Maize, Niranjan Baisakh, Eduardo A. Da Silva, Anjan K. Pradhan, Kanniah Rajasekaran

Faculty Publications

Aflatoxin (AF) contamination, caused by Aspergillus flavus, compromises the food safety and marketability of commodities, such as maize, cotton, peanuts, and tree nuts. Multigenic inheritance of AF resistance impedes conventional introgression of resistance traits into high-yielding commercial maize varieties. Several AF resistance-associated quantitative trait loci (QTLs) and markers have been reported from multiple biparental mapping and genome-wide association studies (GWAS) in maize. However, QTLs with large confidence intervals (CI) explaining inconsistent phenotypic variance limit their use in marker-assisted selection. Meta-analysis of published QTLs can identify significant meta-QTLs (MQTLs) with a narrower CI for reliable identification of genes and linked markers …