Digital Commons Network^™

Admixture Mapping Provides Evidence Of Association Of The Vnn1 Gene With Hypertension, Xiaofeng Zhu, Richard S. Cooper

Faculty Publications

Migration patterns in modern societies have created the opportunity to use population admixture as a strategy to identify susceptibility genes. To implement this strategy, we genotyped a highly informative ancestry marker panel of 2270 single nucleotide polymorohisms in a random nonulation sample of African American (N = 1743). European Americans (N = 1000) and Mexican Americans (N = 581). We then examined the evidence for over-transmission of specific loci to cases from one of the two ancestral populations. Hypertension cases and controls were defined based on standard clinical criteria. Both case-only and case-control analyses were performed among African Americans. With …

Social Class And Belonging: Implications For College Adjustment, Joan Ostrove

Faculty Publications

This study addressed the extent to which social class position structures a sense of belonging at college, and the ways in which belonging informs adjustment to college. Among 322 liberal arts college students, social class background was significantly associated with a sense of belonging at college and was marginally related to academic performance but was not related to the quality of the experience they were having at college. Sense of belonging mediated the relationship between class background and adjustment to college.

Improving Protein Crystal Quality By Selective Removal Of A Ca 2+-Dependent Membrane-Insertion Loop, David B. Neau, Nathaniel C. Gilbert, Sue G. Bartlett, Adam Dassey, Marcia E. Newcomer

Faculty Publications

Lipoxygenases (LOXs) catalyze the regiospecific and stereospecific dioxygenation of polyunsaturated membrane-embedded fatty acids. A Ca 2+-dependent membrane-binding function was localized to the amino-terminal C2-like domain of 8R-lipoxygenase (8R-LOX) from the soft coral Plexaura homomalla. The 3.2 Å crystal structure of 8R-LOX and spectroscopic data suggested that Ca2+ stabilizes two membrane-insertion loops. Analysis of the protein packing contacts in the crystal lattice indicated that the conformation of one of the two loops complicated efforts to improve the resolution of the X-ray data. A deletion mutant of 8R-LOX in which the corresponding membrane-insertion loop is absent (Δ41-45:GSLOX) was engineered. Removal of the …

Outcomes Of Religious And Spiritual Adaptations To Psychotherapy: A Meta-Analytic Review, Timothy B. Smith, Jeremy Bartz, P. Scott Richards

Faculty Publications

The use of spiritually oriented psychotherapies has increased dramatically during the past decade. This article reports a meta-analysis of 31 outcome studies of spiritual therapies conducted from 1984 to 2005 with clients suffering from a variety of psychological problems. Across the 31 studies, the random-effects weighted average effect size was 0.56. This finding provides some empirical evidence that spiritually oriented psychotherapy approaches may be beneficial to individuals with certain psychological problems (e.g., depression, anxiety, stress, eating disorders). Recommendations for future research in this domain are offered.

Who Says You're Disabled? The Role Of Medical Evidence In The Ada Definition Of Disability, Deirdre M. Smith

Faculty Publications

The Americans with Disabilities Act (ADA), enacted by Congress seventeen years ago, offered disabled people a hope of equality and access that has not been fulfilled. ¹ Court decisions halt an overwhelming majority of claims, particularly in the employment context, at the summary judgment stage. ² A key mechanism for fencing out disabled people's claims is the pernicious requirement, based upon the very construction of disability that the ADA's proponents aimed to dispel, that medical evidence is required as a threshold matter to demonstrate that the plaintiff is entitled to seek protection under the statute. ³ The medical evidence requirement …

Role Of Adiponectin And Inflammation In Insulin Resistance Of Mc3r And Mc4r Knockout Mice, James L. Trevaskis, Barbara Gawronska-Kozak, Gregory M. Sutton, Michele Mcneil, Jacqueline M. Stephens, Steven R. Smith, Andrew A. Butler

Faculty Publications

Objective: To investigate the involvement of hypoadiponectinemia and inflammation in coupling obesity to insulin resistance in melanocortin-3 receptor and melanocortin-4 receptor knockout (KO) mice (Mc3/4rKO). Research Methods and Procedures: Sera and tissue were collected from 6-month-old Mc3rKO, Mc4rKO, and wild-type C57BL6J litter mates maintained on low-fat diet or exposed to high-fat diet (HFD) for 1 or 3 months. Inflammation was assessed by both real-time polymerase chain reaction analysis of macrophage-specific gene expression and immunohistochemistry. Results: Mc4rKO exhibited hypoadiponectinemia, exacerbated by HFD and obesity, previously reported in murine models of obesity. Mc4r deficiency was also associated with high levels of macrophage …

Capillary Electrophoretic Screening For The Inhibition Of Homocysteine Thiolactone-Induced Protein Oligomerization, Arther T. Gates, Mark Lowry, Kristin A. Fletcher, Abitha Murugeshu, Oleksandr Rusin, James W. Robinson, Robert M. Strongin, Isiah M. Warner

Faculty Publications

We report the first demonstration of rapid electrophoretic monitoring of homocysteine thiolactone-induced protein oligomerization (HTPO), a unique type of post-translational protein modification that may have clinical significance as an indicator of cardiovascular and neurovascular diseases. HTPO of the model protein bovine cytochrome c was initiated in vitro. The relative monomer and aggregate levels of the resultant protein mixtures were determined following separation using capillaries coated with the cationic polymer, poly(diallyldimethylammonium chloride). UV detection provided adequate sensitivity for the monitoring of higher order species, which exist at relatively low concentrations in the protein reaction mixture as compared to the monomelic species. …

Calcium-Dependent Fast Depolarizing Afterpotentials In Vasopressin Neurons In The Rat Supraoptic Nucleus, Ryoichi Teruyama, William E. Armstrong

Faculty Publications

Oxytocin (OT) and vasopressin (VP) synthesizing magnocellular cells (MNCs) in the supraoptic nucleus (SON) display distinct firing patterns during the physiological demands for these hormones. Depolarizing afterpotentials (DAPs) in these neurons are involved in controlling phasic bursting in VP neurons. Our whole cell recordings demonstrated a Cs+-resistant fast DAP (fDAP; decay tau = ∼200 ms), which has not been previously reported, in addition to the well-known Cs+-sensitive slower DAP (sDAP; decay tau = ∼2 s). Immunoidentification of recorded neurons revealed that all VP neurons, but only 20% of OT neurons, expressed the fDAP. The activation of the fDAP required influx …

Cloning, Analysis And Functional Annotation Of Expressed Sequence Tags From The Earthworm Eisenia Fetida, Mehdi Pirooznia, Ping Gong, Xin Guan, Laura S. Inouye, Kuan Yang, Edward J. Perkins, Youping Deng

Faculty Publications

Background

Eisenia fetida, commonly known as red wiggler or compost worm, belongs to the Lumbricidae family of the Annelida phylum. Little is known about its genome sequence although it has been extensively used as a test organism in terrestrial ecotoxicology. In order to understand its gene expression response to environmental contaminants, we cloned 4032 cDNAs or expressed sequence tags (ESTs) from two E. fetida libraries enriched with genes responsive to ten ordnance related compounds using suppressive subtractive hybridization-PCR.

Results

A total of 3144 good quality ESTs (GenBank dbEST accession number EH669363–EH672369 and EL515444–EL515580) were obtained from the raw clone …

The Fema Trailer Parks: Negative Perceptions And The Social Structure Of Avoidance, Matthew R. Lee, Frederick D. Weil, Edward S. Shihadeh

Faculty Publications

In recent years, negative media attention has fostered the impression that Federal Emergency Management Agency (FEMA) parks are social wastelands filled with criminal elements and other undesirables. FEMA parks have subsequently come to be viewed by some as a major threat to the safety and quality of life of the local communities in which they are situated. This analysis addresses attitudes toward FEMA trailer parks in Baton Rouge after Hurricane Katrina among Baton Rouge residents. Drawing on the Locally Undesirable Land Use/Not in My Backyard (LULU/NIMBY) literature, the contact hypothesis, and a broad paradigm of social status and social control, …

Influence Of Fat-Replacing Ingredients On Process And Age Induced Soluble Nitrogen Content And Ultrastructure Of Lowfat Cheddar Cheese, Zahur U. Haque, Erdogan Küçüköner, Kayanush J. Aryana

Faculty Publications

Influence of fat-replacers on process and age (up to 6 months at 5°C) induced water soluble nitrogen generation in lowfat (5%, w/w) Cheddar cheese was investigated. Treatments with cellulose plus guar gum based Novagel NC200 (NOV), starch based Stellar (STEL), and protein based Dairy Lo (DL) and Simplesse (SIMP) were compared with lowfat (LFC) and fullfat (32%) controls. Nitrogen contents in aqueous extract were determined by Kjeldahl method. Process induced proteolysis followed the order DL > NOV > SIMP > STEL > LFO FFC. Age related proteolysis, which generated hydrophobic peptides, was rapid in the first 3 months, and resulted in an increase of …

Associations Among Physical Activity, Health Indicators, And Employment In 12th Grade Girls, Marsha Dowda, Karin A. Pfeiffer, Rod K. Dishman, Russell R. Pate

Faculty Publications

Objectives: This study compared physical activity, sedentary behaviors, and other health indicators between 1381 employed and nonemployed 12th grade girls.

Methods: The girls were from 22 high schools in South Carolina (2002-2003); 56% of the girls were African American, and the mean age was 17.7 (0.6) years. Physical activity and sedentary behaviors were measured using the 3-Day Physical Activity Recall (3DPAR). Fitness, depressive symptoms, and smoking behavior were assessed.

Results: Fifty percent of the girls were employed, and on average, employed girls worked 9.6 30-minute blocks per day. Girls who worked reported significantly (p≤0.001) higher average total metabolic …

Undergraduate Use Of Federated Searching: A Survey Of Preferences And Perceptions Of Value-Added Functionality, C. Jeffrey Belliston, Jared L. Howland, Brian C. Roberts

Faculty Publications

Randomly selected undergraduates at Brigham Young University, Brigham Young University-Idaho and Brigham Young University-Hawaii, all private universities sponsored by The Church of Jesus Christ of Latter-day Saints, participated in a study that investigated four questions regarding federated searching: (1) Does it save time? (2) Do undergraduates prefer it? (3) Are undergraduates satisfied with the results they get from it? (4) Does it yield higher quality results than non-federated searching? Federated searching was, on average, 11% faster than non-federated searching. Undergraduates rated their satisfaction with the citations gathered by federated searching 17% higher than their satisfaction using non-federated search methods. A …

Comparison Of A Low Carbohydrate And Low Fat Diet For Weight Maintenance In Overweight Or Obese Adults Enrolled In A Clinical Weight Management Program, James D. Lecheminant, Cheryl A. Gibson, Debra K. Sullivan, Sandra Hall, Rik Washburn, Mary C. Vernon, Chelsea Curry, Elizabeth Stewart, Eric C. Westman, Joseph E. Donnelly

Faculty Publications

Background: Recent evidence suggests that a low carbohydrate (LC) diet may be equally or more effective for short-term weight loss than a traditional low fat (LF) diet; however, less is known about how they compare for weight maintenance. The purpose of this study was to compare body weight (BW) for participants in a clinical weight management program, consuming a LC or LF weight maintenance diet for 6 months following weight loss. Methods: Fifty-five (29 low carbohydrate diet; 26 low fat diet) overweight/obese middle-aged adults completed a 9 month weight management program that included instruction for behavior, physical activity (PA), and …

Wireless Power Transmission To A Buried Sensor In Concrete, Khan M.Z. Shams, Mohammod Ali

Faculty Publications

The feasibility of sending wireless power to a buried sensor antenna within concrete was studied. a receive patch rectenna with 75.8% conversion efficiency was designed for operation at 5.7 GHz. The received DC power at the rectenna was measured within dry and wet concrete samples with various cover thicknesses and air-gaps. For the rectenna buried within 30 mm of the concrete, the received DC power was 10.37 mW, which was, about 70% of the received DC power in free-space.

School-Based Mentoring: An Effective Intervention With Students At Risk For Ebd, Paul Caldarella, Shauna Valentine, K. Richard Young, Michael Adams

Faculty Publications

Relationships with non-parental adults are vital assets for positive youth development (Scales, 2003). Studies of resilience among youth from at risk backgrounds alerted scholars to protective functions that can be fulfilled by relationships with non parental adults (Werner, 1995).

The Effects Of Peer Praise Notes On Socially Withdrawn Adolescents: A Classroom Intervention, Julie Nelson, Natalie Webb, Paul Caldarella

Faculty Publications

Relationships with non-parental adults are vital assets for positive youth development (Scales, 2003). Studies of resilience among youth from at risk backgrounds alerted scholars to protective functions that can be fulfilled by relationships with non parental adults (Werner, 1995)

The Impact Of Maternal Relationship Quality On Emerging Adults’ Prosocial Tendencies: Indirect Effects Via Regulation Of Prosocial Values, Carolyn Mcnamara Barry, Laura M. Padilla-Walker, Stephanie D. Madsen, Larry J. Nelson

Faculty Publications

Studies document that parents serve as children’s primary socialization agents, particularly for moral development and prosocial behavior; however, less is known regarding parental influences on prosocial outcomes during the transition to adulthood. The purpose of this study was to investigate how mother–child relationship quality was related to prosocial tendencies via emerging adults’ regulation of prosocial values. Participants included 228 undergraduate students (ranging from 18 to 25 years; 90% European American) and their mothers (ranging from 38 to 59 years) from four locations across the United States. Path analyses using structural equation modeling revealed that mother–child relationship quality was related to …

Looking On The Bright Side: The Role Of Identity Status And Gender On Positive Orientations During Emerging Adulthood, Laura M. Padilla-Walker, Carolyn Mcnamara Barry, Jason S. Carroll, Stephanie D. Madsen, Larry J. Nelson

Faculty Publications

Emerging adulthood has been characterized as an age of possibilities that involves heightened identity exploration and risk-taking. Although some scholars have investigated the relation between identity status and risk behaviors in emerging adulthood, less attention has been paid to the relation between identity status and prosocial orientations. Thus, the current study examined how emerging adults’ engagement in positive behaviors (i.e., prosocial tendencies, internalization of values, religiosity) varied as a function of identity status and gender. Results indicated that emerging adults who were in identity diffusion reported fewer prosocial tendencies, lower scores on internalization of values and lower religiosity, but emerging …

Spiritual Interventions In Psychotherapy: Evaluations By Highly Religious Clients, Jennifer S. Martinez, Timothy B. Smith, Sally H. Barlow

Faculty Publications

Spiritual and religious interventions in psychotherapy have increasingly received research attention, particularly with highly religious clients. This study examined client opinions about and experiences with religious interventions in psychotherapy. A sample of 152 clients at a counseling center of a university sponsored by the Church of Jesus Christ of Latter-day Saints (LDS) completed a survey with ratings of specific religious interventions with regards to appropriateness, helpfulness, and prevalence. Out-of-session religious interventions were considered more appropriate by clients than in-session religious interventions, but in-session interventions were rated as more helpful. Specific interventions considered both appropriate and helpful by the LDS participants …

Practical Consequences, Institutional Competence, And The Kentucky Bond Case, Bradley W. Joondeph

Faculty Publications

On November 5, 2007, the Supreme Court will hear arguments in Department of Revenue of Ky. v. Davis. At issue is whether state income tax preferences for their own municipal bonds impermissibly discriminate against interstate commerce. This article makes two basic arguments as to why the Court should find such preferences constitutional. First, as a doctrinal matter, Davis is essentially indistinguishable from the Court's decision last term in United Haulers Assn., Inc. v. Oneida-Herkimer Solid Waste Mgmt. Auth. There, the Court held that laws that "favor the government," that "treat every private business. . . exactly the same," and that …

The Association Of Racial Attitudes And Spiritual Beliefs In Post-Apartheid South Africa, Timothy B. Smith, Christopher R. Stones, Christopher E. Peck, Anthony V. Naidoo

Faculty Publications

Previous research has investigated the complex association between religious beliefs and racism. Many studies have found that fundamentalist religious beliefs are positively associated with racial prejudice among European and European American populations. However, few studies have examined whether this association is found in other cultures or whether the association also characterizes spiritual beliefs. Data from 493 South African university students from three racial backgrounds revealed significant differences among the groups. A positive association between fundamentalism and racial prejudice was found among participants, but general spiritual beliefs were negatively associated with racist attitudes. The results emphasize the need to address contextual …

The Chlamydomonas Genome Reveals The Evolution Of Key Animal And Plant Functions, Sabeeha S. Merchant, Simon E. Prochnik, Olivier Vallon, Elizabeth H. Harris, Steven J. Karpowicz, George B. Witman, Astrid Terry, Asaf Salamov, Lillian K. Fritz-Laylin, Laurence Maréchal-Drouard, Wallace F. Marshall, Liang Hu Qu, David R. Nelson, Anton A. Sanderfoot, Martin H. Spalding, Vladimir V. Kapitonov, Qinghu Ren, Patrick Ferris, Erika Lindquist, Harris Shapiro, Susan M. Lucas, Jane Grimwood, Jeremy Schmutz, Igor V. Grigoriev, Daniel S. Rokhsar, Arthur R. Grossman, Pierre Cardol, Heriberto Cerutti, Guillaume Chanfreau, Chun Long Chen, Valérie Cognat, Martin T. Croft, Rachel Dent

Faculty Publications

Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It is a model system for studying chloroplast-based photosynthesis, as well as the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited from the common ancestor of plants and animals, but lost in land plants. We sequenced the ∼120-megabase nuclear genome of Chlamydomonas and performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated with the function and biogenesis of chloroplasts or eukaryotic flagella. Analyses of the Chlamydomonas genome advance our understanding of the ancestral eukaryotic cell, …

Habitat Fragmentation, Variable Edge Effects, And The Landscape-Divergence Hypothesis, William F. Laurance, Henrique E.M. Nascimento, Susan G. Laurance, Ana Andrade, Robert M. Ewers, Kyle E. Harms, Regina C.C. Luizão, José E. Ribeiro

Faculty Publications

Edge effects are major drivers of change in many fragmented landscapes, but are often highly variable in space and time. Here we assess variability in edge effects altering Amazon forest dynamics, plant community composition, invading species, and carbon storage, in the world's largest and longest-running experimental study of habitat fragmentation. Despite detailed knowledge of local landscape conditions, spatial variability in edge effects was only partially foreseeable: relatively predictable effects were caused by the differing proximity of plots to forest edge and varying matrix vegetation, but windstorms generated much random variability. Temporal variability in edge phenomena was also only partially predictable: …

Sammd: Staphylococcus Aureus Microarray Meta-Database, Vijayaraj Nagarajan, Mohamed O. Elasri

Faculty Publications

Background

Staphylococcus aureus is an important human pathogen, causing a wide variety of diseases ranging from superficial skin infections to severe life threatening infections. S. aureus is one of the leading causes of nosocomial infections. Its ability to resist multiple antibiotics poses a growing public health problem. In order to understand the mechanism of pathogenesis of S. aureus, several global expression profiles have been developed. These transcriptional profiles included regulatory mutants of S. aureus and growth of wild type under different growth conditions. The abundance of these profiles has generated a large amount of data without a uniform annotation …

Treatment Of Legal Fees Incurred By Individuals, Annette M. Nellen

Faculty Publications

If the origin of a claim that generated legal fees is personal, the fees are not deductible. Possible favorable treatment for legal fees includes either above-the-line deductions or adjustments to basis or selling price in a property transaction. Some taxpayers have claimed described legal fees in such a way that they directly reduce the related income.

Changing Course: Navigating Life After Fifty, E Michael Brady Phd

Faculty Publications

The article reviews the book Changing Course: Navigating Life After Fifty, by William A. Sadler and James H. Kreft.

Consideration Of 'Contracting Culture' In Enforcing Arbitration Provisions, Amy J. Schmitz

Faculty Publications

The Federal Arbitration Act mandates strict and uniform enforcement of standardized pre-dispute arbitration provisions. This may not be proper, however, in light of the importance of context with respect to these provisions. This Article therefore seeks to remind courts of the importance of exchange context by proposing a "contracting culture" continuum for enforcing these arbitration provisions that acknowledges the impacts of these provisions in a particular communal context. "Contracting culture" encompasses economic and non-economic relational factors that impact dispute resolution agreements, but go beyond common conceptions of "culture" focused on ethnicity, nationality, or religion. It also explores beyond the primary …

When Accommodations For Religion Violate The Establishment Clause: Regularizing The Supreme Court's Analysis, Carl H. Esbeck

Faculty Publications

This article sets forth five rules with respect to what government may do to accommodate religious practice and five rules with respect to what government may not do. As it turns out the Supreme Court has said that most religious accommodations are left to the broad discretion of legislators and public officials. So long as the object of the accommodation is to protect or expand religious freedom, as distinct from expanding religion, the accommodation will be permitted.

Requirement Of Α And Β Subunit Transmembrane Helix Separation For Integrin Outside-In Signaling, Jieqing Zhu, Christopher V. Carman, Minsoo Kim, Motomu Shimaoka, Timothy A. Springer, Bing Hao Luo

Faculty Publications

Adhesion to extracellular ligands through integrins regulates cell shape, migration, growth, and survival. How integrins transmit signals in the outside-to-in direction remains unknown. Whereas in resting integrins the α and β subunit transmembrane domains are associated, ligand binding promotes dissociation and separation of these domains. Here we address whether such separation is required for outside-in signaling. By introduction of an intersubunit disulfide bond, we generated mutant integrin αIIbβ3 with blocked transmembrane separation that binds ligand, mediates adhesion, adopts an extended conformation after ligand binding, and forms antibody-induced macroclusters on the cell surface similarly to wild type. However, the mutant integrin …