Digital Commons Network^™

A Structural Interpretation Of The Effect Of Gc-Content On Efficiency Of Rna Interference, Chi Yu Chan, C Steven Carmack, Dang D. Long, Anil Maliyekkel, Yu Shao, Igor Roninson, Ye Ding

Faculty Publications

BACKGROUND: RNA interference (RNAi) mediated by small interfering RNAs (siRNAs) or short hairpin RNAs (shRNAs) has become a powerful technique for eukaryotic gene knockdown. siRNA GC-content negatively correlates with RNAi efficiency, and it is of interest to have a convincing mechanistic interpretation of this observation. We here examine this issue by considering the secondary structures for both the target messenger RNA (mRNA) and the siRNA guide strand. RESULTS: By analyzing a unique homogeneous data set of 101 shRNAs targeted to 100 endogenous human genes, we find that: 1) target site accessibility is more important than GC-content for efficient RNAi; 2) …

Antenatal Peer Support Workers And Initiation Of Breast Feeding: Cluster Randomised Controlled Trial, Christine Macarthur, Kate Jolly, Lucy Annang Ingram Phd, Nick Freemantle, Cindy-Lee Dennis, Ros Hamburger, Julia Brown, Jackie Chambers, Khalid Khan

Faculty Publications

OBJECTIVE: To assess the effectiveness of an antenatal service using community based breastfeeding peer support workers on initiation of breast feeding. DESIGN: Cluster randomised controlled trial. SETTING: Community antenatal clinics in one primary care trust in a multiethnic, deprived population. PARTICIPANTS: 66 antenatal clinics with 2511 pregnant women: 33 clinics including 1140 women were randomised to receive the peer support worker service and 33 clinics including 1371 women were randomised to receive standard care. INTERVENTION: An antenatal peer support worker service planned to comprise a minimum of two contacts with women to provide advice, information, and support from approximately 24 …

Does Television Viewing Predict Dietary Intake Five Years Later In High School Students And Young Adults?, Daheia J. Barr-Anderson, Nicole I. Larson, Melissa C. Nelson, Dianne R. Neumark-Sztainer, Mary Story

Faculty Publications

Background: Prior research has found that television viewing is associated with poor diet quality, though little is known about its long-term impact on diet, particularly during adolescence. This study examined the associations between television viewing behavior with dietary intake five years later.

Methods: Survey data, which included television viewing time and food frequency questionnaires, were analyzed for 564 middle school students (younger cohort) and 1366 high school students (older cohort) who had complete data available at Time1 (1998-1999) and five years later at Time 2 (mean age at Time 2, 17.2 ± 0.6 and 20.5 ± 0.8 years, respectively). Regression …

Dietary Resistant Starch Increases Hypothalamic Pomc Expression In Rats, Li Shen, Michael J. Keenan, Roy J. Martin, Richard T. Tulley, Anne M. Raggio, Kathleen L. Mccutcheon, Jun Zhou

Faculty Publications

Resistant starch (RS) is fermentable dietary fiber. Inclusion of RS in the diet causes decreased body fat accumulation and altered gut hormone profile. This study investigates the effect of feeding RS on the neuropeptide messenger RNA (mRNA) expressions in the arcuate nucleus (ARC) of the hypothalamus and whether vagal afferent nerves are involved. The rats were injected intraperitoneally with capsaicin to destroy unmyelinated small vagal afferent nerve fibers. The cholecystokinin (CCK) food suppression test was performed to validate the effectiveness of the capsaicin treatment. Then, capsaicin-treated rats and vehicle-treated rats were subdivided into a control diet or a RS diet …

Race And Ancestry In Biomedical Research: Exploring The Challenges, Timothy Caulfield, Stephanie M. Fullerton, Sarah E. Ali-Khan, Laura Arbour, Esteban G. Burchard, Richard S. Cooper, Billie Jo Hardy, Simrat Harry, Robyn Hyde-Lay, Jonathan Kahn, Rick Kittles, Barbara A. Koenig, Sandra S.J. Lee, Michael Malinowski, Vardit Ravitsky, Pamela Sankar, Stephen W. Scherer, Béatrice Séguin, Darren Shickle, Guilherme Suarez-Kurtz, Abdallah S. Daar

Faculty Publications

The use of race in biomedical research has, for decades, been a source of social controversy. However, recent events, such as the adoption of racially targeted pharmaceuticals, have raised the profile of the race issue. In addition, we are entering an era in which genomic research is increasingly focused on the nature and extent of human genetic variation, often examined by population, which leads to heightened potential for misunderstandings or misuse of terms concerning genetic variation and race. Here, we draw together the perspectives of participants in a recent interdisciplinary workshop on ancestry and health in medicine in order to …

Flow Cytometric And Immunohistochemical Detection Of In Vivo Brdu-Labeled Cells In Mouse Fat Depots, Jaroslaw Staszkiewicz, Jeffrey Gimble, Courtney Cain, Marilyn Dietrich, David Burk, Heather Kirk-Ballard, Barbara Gawronska-Kozak

Faculty Publications

This study has determined the natural frequency and localization of progenitor/stem cells within fat depots in situ based on their ability to retain DNA nucleotide label (BrdU). Neonate and mature male C57BL6/J mice were injected intraperitoneally with BrdU- and label-retaining cells (LRC) were quantified in fat depots by immunohistochemical, immunofluorescent, and flow cytometric methods. In neonates, LRC constituted 27% of the cells in inguinal fat (iWAT) and 65% in interscapular brown fat (BAT) after Day 10 and 26% of the cells in epididymal fat (eWAT) after Day 28. After 52 days, the LRC accounted for 0.72% of iWAT, 0.53% of …

Longitudinal Changes In Global Brain Volume Between 79 And 409 Days After Traumatic Brain Injury: Relationship With Duration Of Coma, Mehul A. Trivedi, Michael A. Ward, Timothy M. Hess, Shawn D. Gale, Robert J. Dempsey, Howard A. Rowley, Sterling C. Johnson

Faculty Publications

Neuropathological and experimental animal studies indicate that traumatic brain injury (TBI) results in long-term, neurodegenerative changes. Structural image evaluation using normalization of atrophy (SIENA) offers an automated analysis of the subtle changes in percent brain volume change (%BVC) associated with TBI. In the present study, SIENA was used to evaluate %BVC in individuals who had sustained a mild to severe TBI. We obtained 3D-T1 weighted anatomical MRI scans approximately 79 days and again 409 days post-injury. TBI patients (n= 37) displayed significantly greater decline in %BVC (-1.43%) relative to a normal comparison group (+ 0.1%, n=30). Greater %BVC was associated …

Structural Characterization Of The Hemophore Hasap From Pseudomonas Aeruginosa: Nmr Spectroscopy Reveals Protein-Protein Interactions Between Holo-Hasap And Hemoglobin, Aileen Y. Alontaga, Juan Carlos Rodriguez, Ernst Schönbrunn, Andreas Becker, Todd Funke, Erik T. Yukl, Takahiro Hayashi, Jordan Stobaugh, Pierre Moënne-Loccoz, Mario Rivera

Faculty Publications

Pseudomonas aeruginosa secretes a 205 residue long hemophore (full-length HasAp) that is subsequently cleaved at the C'-terminal domain to produce mainly a 184 residue long truncated HasAp that scavenges heme [Letoffé, S., Redeker, V., and Wandersman, C. (1998) Mol. Microbiol. 28, 1223-1234]. HasAp has been characterized by X-ray crystallography and in solution by NMR spectroscopy. The X-ray crystal structure of truncated HasAp revealed a polypeptide αβ fold and a ferriheme coordinated axially by His32 and Tyr75, with the side chain of His83 poised to accept a hydrogen bond from the Tyr75 phenolic acid group. NMR investigations conducted with full-length HasAp …

Are Biogenic Emissions A Significant Source Of Summertime Atmospheric Toluene In The Rural Northeastern United States?, Marguerite L. White, Rachel S. Russo, Yong Zhou, Jesse L. Ambrose, Karl B. Haase, E. K. Frinak, Ruth K. Varner, Oliver W. Wingenter, Huiting Mao, Robert W. Talbot, Barkley C. Sive

Faculty Publications

Summertime atmospheric toluene enhancements at Thompson Farm in the rural northeastern United States were unexpected and resulted in a toluene/benzene seasonal pattern that was distinctly different from that of other anthropogenic volatile organic compounds. Consequently, three hydrocarbon sources were investigated for potential contributions to the enhancements during 2004–2006. These included: (1) increased warm season fuel evaporation coupled with changes in reformulated gasoline (RFG) content to meet US EPA summertime volatility standards, (2) local industrial emissions and (3) local vegetative emissions. The contribution of fuel evaporation emission to summer toluene mixing ratios was estimated to range from 16 to 30 pptv …

Whole-Genome Association Study Identifies Stk39 As A Hypertension Susceptibility Gene, Ying Wang, Jeffrey R. O'Connell, Patrick F. Mcardle, James B. Wade, Sarah E. Dorff, Sanjiv J. Shah, Xiaolian Shi, Lin Pan, Evadnie Rampersaud, Haiqing Shen, James D. Kim, Arohan R. Subramanya, Nanette I. Steinle, Afshin Parsa, Carole C. Ober, Paul A. Welling, Aravinda Chakravarti, Alan B. Weder, Richard S. Cooper, Braxton D. Mitchell, Alan R. Shuldiner, Yen Pei C. Chang

Faculty Publications

Hypertension places a major burden on individual and public health, but the genetic basis of this complex disorder is poorly understood. We conducted a genome-wide association study of systolic and diastolic blood pressure (SBP and DBP) in Amish subjects and found strong association signals with common variants in a serine/threonine kinase gene, STK39. We confirmed this association in an independent Amish and 4 non-Amish Caucasian samples including the Diabetes Genetics Initiative, Framingham Heart Study, GenNet, and Hutterites (meta-analysis combining all studies: n = 7,125, P < 10-6). The higher BP-associated alleles have frequencies > 0.09 and were associated with increases of 3.3/1.3 mm Hg in SBP/DBP, respectively, in the …

Health Effects Of Vegan Diets, Winston J. Craig

Faculty Publications

Recently, vegetarian diets have experienced an increase in popularity. Avegetarian diet is associated with many health benefits because of its higher content of fiber, folic acid, vitamins C and E, potassium, magnesium, and many phytochemicals and a fat content that is more unsaturated. Compared with other vegetarian diets, vegan diets tend to contain less saturated fat and cholesterol and more dietary fiber. Vegans tend to be thinner, have lower serum cholesterol, and lower blood pressure, reducing their risk of heart disease. However, eliminating all animal products from the diet increases the risk of certain nutritional deficiencies. Micronutrients of special concern …

Tolerance, Democracy And Fundamentalism(S) : Challenges In Time Of Systemic Bifurcations, Guillermo C. Hansen

Faculty Publications

No abstract provided.

Rural School Principals’ Perception Of The School Counselor's Role, Gerta Bardhoshi, Kelly Duncan

Faculty Publications

Responding to the lack of clarity related to the defined role of the school counselor, the authors investigated school principals (n = 538), in a rural Midwest state, and their perceptions of the role of the school counselor. The survey utilized was developed based on professional standards of service delivery for professional school counselors as identified by the American School Counselor Association and a listing of identified appropriate and inappropriate school counselor tasks. Results indicate that school principals see responsive service provision as an essential task of the school counselor. School principals also perceive a number of ASCA identified inappropriate …

How Can A Video Game Cause Panic Attacks? 1. Effects Of An Auditory Stressor On The Human Brainstem, Judith Lauter, Elizabeth Mathukutty, Brandon Scott

Faculty Publications

The auditory brainstem response (ABR) was recorded during simultaneous binaural presentation of two types of sounds: 1) condensation clicks presented through in-the-ear earphones at 43.1/sec, 60dB nHL; and 2) recordings of breathing sounds, presented through supra-aural headphones, at levels adjusted by participants to be equivalent to the clicks. In alternate blocks, the breathing sounds were either: 1) a recording of quiet breathing (blocks 1, 3, 5); or 2) a recording of erratic (stressed) breathing (blocks 2, 4). The erratic breathing was modeled on a video game soundtrack in which the character was represented as running, wounded, and frightened. Four 2048-sweep …

Speaking Into Silences: Autoethnography, Communication, And Applied Research, Lisa M. Tillmann Ph.D.

Faculty Publications

In 2004, two articles in the Journal of Applied Communication Research (Ashcraft & Tretheway, 2004; Goodall, 2004) celebrated the merits of auto- and narrative ethnography, methods of research grounded in lived experience and evocative modes of representation that seek to engage readers emotionally, aesthetically, ethically, and politically. Despite these and other persuasive calls for auto- and narrative ethnographic works, few have been published in communication journals. More than four years ago, JACR offered readers arguments for this kind of scholarship, yet no full-length autoethnography appeared in its pages—until now. This article, a prelude to its companion essay, “Body and Bulimia …

Performance Comparison Between The Use And Nonuse Of An Enteric Health Medication Program Across Five Consecutive Commercial Broiler Flocks, Joey Lynn Bray, C. S. Taylor, T. E. Cherry, J. Carey

Faculty Publications

A study was conducted to examine broiler performance and carcass yield across 5 consecutive commercial broiler flocks after the removal of roxarsone (ROX) and growth-promoting antibiotics (GPA) from the feed. Over a 1-yr period, approximately 552,000 broilers were reared in 4 solid-walled, tunnel-ventilated houses, divided into 2 paired-house facilities, and were assigned 1 of 2 dietary treatments. The treated group received basal diets containing salinomycin, ROX, and GPA, whereas the control group received the same diets without ROX and GPA. Average BW were recorded for 200 sample birds/treatment per flock at 18, 35, and 48 d of age. Average BW, …

Calls For Accountability: Will It Help The Overall Incentives Process?, Annette M. Nellen

Faculty Publications

No abstract provided.

The Effects Of Different Types And Patterns Of Services On Successful Reunification, California Social Work Education Center (Calswec), Amy D'Andrade

Faculty Publications

No abstract provided.

Body And Bulimia Revisited: Reflections On "A Secret Life", Lisa M. Tillmann Ph.D.

Faculty Publications

In 1996, the author published “A Secret Life in a Culture of Thinness: Reflections on Body, Food, and Bulimia” (Tillmann-Healy, 1996), an account of her struggle with binging and purging from ages 15 to 25. She came to understand bulimia as a communicative act, expressing fear, anxiety, and grief. From 25 to 35, her recovery from bulimia involved learning to “purge” emotion through other forms of communication (e.g., dialogue, writing, and teaching). At 35, separation and divorce pose the greatest challenge to the author’s 10-year recovery, yet she does not return to bulimic expression. This article invites readers to sense …

Demographics Of A Geographically-Isolated Population Of Threatened Salamander (Caudata: Ambystomatidae) In Central Illinois, Stephen J. Mullin, Sarabeth Klueh

Faculty Publications

Amphibian populations that use small isolated wetlands are often small in size, susceptible to stochastic extinction processes, and have little to no contact with other populations. One can ascertain the persistence of such populations only by obtaining data that allow the prediction of future changes in population’s size, and propensity to achieve a sustainable number of individuals. The number of metamorphosing larvae leaving a pond predicts the viability of a salamander population, and thus, the number recruited into the terrestrial adult population. The Jefferson Salamander, Ambystoma jeffersonianum, is a listed threatened species in Illinois, occurring at fewer than 15 ponds …

Singlet Oxygen Delivery Through The Porous Cap Of A Hollow-Core Fiber Optic Device, Matibur Zamadar, David Aebisher, Alexander Greer

Faculty Publications

The development of the first photosensitizer/fiber optic device is reported. An oxygen-flowing, fiber-capped configuration is used for the application of heterogeneous, spatially confined singlet oxygen delivery in aqueous media. This is a unique device, unlike other heterogeneous photosensitizers, in which local concentrations of singlet oxygen can be delivered via introduction and withdrawal of the fiber tip.

Policing Miscarriage: Infertility Blogging, Rhetorical Enclaves, And The Case Of House Bill 1677, Clancy Ratliff

Faculty Publications

No abstract provided.

It’S Just A Shot Away: Mmr Vaccines And Autism And The End Of The Daubertista Revolution, Joelle A. Moreno

Faculty Publications

No abstract provided.

Does A Gatekeeper Suicide Prevention Program Work In A School Setting? Evaluating Training Outcome And Moderators Of Effectiveness, Tanya L. Tompkins, Jody Witt, Nadia Abraibesh

Faculty Publications

The current study sought to evaluate the suicide prevention gatekeeper training program QPR (Question, Persuade, and Refer) among school personnel using a non-equivalent control group design. Substantial gains were demonstrated from pre- to post-test for attitudes, knowledge, and beliefs regarding suicide and suicide prevention. Exploratory analyses revealed the possible moderating effects of age, professional role, prior training, and recent contact with suicidal youth on QPR participants’ general knowledge, questioning, attitudes toward suicide and suicide prevention, QPR quiz scores, and self-efficacy. The need for replication using a more rigorous experimental design in the context of strong community collaboration is discussed.

The Short-Term Effectiveness Of A Suicide Prevention Gatekeeper Training Program In A College Setting With Residence Life Advisers, Tanya L. Tompkins, Jody Witt

Faculty Publications

Although the college years prove to be a vulnerable time for students and a critical period for suicide prevention, few school-based prevention strategies have been empirically evaluated. The current study examined the short-term effects of QPR (Question, Persuade, and Refer), a gatekeeper training program that teaches how to recognize warning signs, question suicidal intent, listen to problems, and refer for help. The 122 residence advisers (RAs) who were trained in QPR demonstrated significant post-training gains across a variety of domains relevant to suicide and suicide prevention, with the 60 completing the follow-up assessment showing sustained knowledge and appraisals into the …

Sunday Friends: The Working Alternative To Charity, James D. Lee, Yoko Baba, Claudio V. Sanchez, Rebecca Wang, Chelsey White

Faculty Publications

Sunday Friends is a non-profit organization in San José, California, that provides multiple activities for families who are in need of financial support. Given the particular location of the program, most families are Latino and bilingual. Participants and program volunteers form a community at an elementary school on a couple of scheduled Sundays each month. When family members participate in activities designed to educate, improve skills, and to give back to the larger community, they earn tickets that they can redeem for items that they need and want from the Sunday Friends store. Activities include healthy cooking projects, “Thank You …

Salt Marshes And Eutrophication: An Unsustainable Outcome, R. Eugene Turner, B L. Howes, J M. Teal, C S. Milan, D D. Goehringer-Toner

Faculty Publications

Most plant production by emergent coastal marshes occurs belowground. This belowground production adds to the accumulation of organic matter sustaining salt marshes as sea level rises, thus preventing excessive flooding, eventual plant death, and habitat loss. The ubiquitous nutrient enrichment of coastal salt marshes stimulating aboveground plant growth may result in higher rates of inorganic matter accumulation that compensates for marsh flooding caused by sea level rise. Results from several short-term experiments, however, demonstrate that root and rhizome biomass and carbon accumulation is reduced with nutrient enrichment, suggesting that eutrophication of coastal waters may not be a compensatory counterbalance to …

Comments On Buzan Et Al. “Positive Relationships Between Freshwater Inflow And Oyster Abundance In Galveston Bay, Texas”, R. Eugene Turner

Faculty Publications

Buzan et al. critique Turner’s (Estuaries and Coasts 29:345–352, 2006) analysis of the relationship between freshwater inflow and oyster productivity in the Gulf of Mexico, using 16 years of fisheries-independent data for Galveston Bay. They conclude that the catch-per-unit effort (CPUE; number h−1) of marketable oysters increase 1 to 2 years after years with increased freshwater inflows, and they express concerns that water supply managers may mis-apply the results of Turner (Estuaries and Coasts 29:345–352, 2006) to justify a reduced freshwater inflow to Galveston Bay. I find no relationship between the CPUE of oyster spat or marketable oyster density and …

Disturbance Impacts On Mercury Dynamics In Northern Gulf Of Mexico Sediments, B. Liu, L.A. Schaider, R.P. Mason, M.S. Bank, N.N. Rabalais, P.W. Swarzenski, J.P. Shine, T. Holiweg, D.B. Senn

Faculty Publications

No abstract provided.

Age Discrimination In The Delivery Of Health Care Services To Our Elders, Phoebe Weaver Williams

Faculty Publications

The problem of health care providers making age biased decisions when treating elderly patients has received considerable attention and condemnation from both medical and social science researchers. When health care providers offer inappropriate or less care to patients because of their advanced chronological age, they potentially violate the Age Act of 1975. However, a review of the cases litigated under the Age Act suggests that advocates, regulators, and elders have not used the Age Act to address even the most blatant ageist practices in health care. Using methods developed to identify unlawful discrimination under employment and civil rights laws, this …