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Mammalian Retroelements, Prescott L. Deininger, Mark A. Batzer Oct 2002

Mammalian Retroelements, Prescott L. Deininger, Mark A. Batzer

Faculty Publications

The eukaryotic genome has undergone a series of epidemics of amplification of mobile elements that have resulted in most eukaryotic genomes containing much more of this 'junk' DNA than actual coding DNA. The majority of these elements utilize an RNA intermediate and are termed retroelements. Most of these retroelements appear to amplify in evolutionary waves that insert in the genome and then gradually diverge. In humans, almost half of the genome is recognizably derived from retroelements, with the two elements that are currently actively amplifying, L1 and Alu, making up about 25% of the genome and contributing extensively to disease. …

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Identification Of A Streptolysin S-Associated Gene Cluster And Its Role In The Pathogenesis Of Streptococcus Iniae Disease, Jeffrey D. Fuller, Alvin C. Camus, Carla L. Duncan, Victor Nizet, Darrin J. Bast, Ronald L. Thune, Donald E. Low, Joyce C.S. De Azavedo Oct 2002

Identification Of A Streptolysin S-Associated Gene Cluster And Its Role In The Pathogenesis Of Streptococcus Iniae Disease, Jeffrey D. Fuller, Alvin C. Camus, Carla L. Duncan, Victor Nizet, Darrin J. Bast, Ronald L. Thune, Donald E. Low, Joyce C.S. De Azavedo

Faculty Publications

Streptococcus iniae causes meningoencephalitis and death in cultured fish species and soft-tissue infection in humans. We recently reported that S. iniae is responsible for local tissue necrosis and bacteremia in a murine subcutaneous infection model. The ability to cause bacteremia in this model is associated with a genetic profile unique to strains responsible for disease in fish and humans (J. D. Fuller, D. J. Bast, V. Nizet, D. E. Low, and J. C. S. de Azavedo, Infect. Immun. 69:1994-2000, 2001). S. iniae produces a cytolysin that confers a hemolytic phenotype on blood agar media. In this study, we characterized the …

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Heca, A Member Of A Class Of Adhesins Produced By Diverse Pathogenic Bacteria, Contributes To The Attachment, Aggregation, Epidermal Cell Killing, And Virulence Phenotypes Of Erwinia Chrysanthemi Ec16 On Nicotiana Clevelandii Seedlings, Clemencia M. Rojas, Jong Hyun Ham, Wen Ling Deng, Jeff J. Doyle, Alan Collmer Oct 2002

Heca, A Member Of A Class Of Adhesins Produced By Diverse Pathogenic Bacteria, Contributes To The Attachment, Aggregation, Epidermal Cell Killing, And Virulence Phenotypes Of Erwinia Chrysanthemi Ec16 On Nicotiana Clevelandii Seedlings, Clemencia M. Rojas, Jong Hyun Ham, Wen Ling Deng, Jeff J. Doyle, Alan Collmer

Faculty Publications

Erwinia chrysanthemi is representative of a broad class of bacterial pathogens that are capable of inducing necrosis in plants. The E. chrysanthemi EC16 hecA gene predicts a 3,850-aa member of the Bordetella pertussis filamentous hemagglutinin family of adhesins. A hecA::Tn7 mutant was reduced in virulence on Nicotiana clevelandii seedlings after inoculation without wounding. Epifluorescence and confocal laser-scanning microscopy observations of hecA and wild-type cells expressing the green fluorescent protein revealed that the mutant is reduced in its ability to attach and then form aggregates on leaves and to cause an aggregate-associated killing of epidermal cells. Cell killing also depended on …

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Between Law And Grace: Ritual And Ritual Studies In Recent Evangelical Thought, Gerald Klingbeil Oct 2002

Between Law And Grace: Ritual And Ritual Studies In Recent Evangelical Thought, Gerald Klingbeil

Faculty Publications

No abstract provided.

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The End Of The Hudson Valley's Peculiar Institution: The Anti-Rent Movement's Politics, Social Relations, & Economics, Eric Kades Oct 2002

The End Of The Hudson Valley's Peculiar Institution: The Anti-Rent Movement's Politics, Social Relations, & Economics, Eric Kades

Faculty Publications

No abstract provided.

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I Am/Was The Walrus, Daniel Beller-Mckenna Sep 2002

I Am/Was The Walrus, Daniel Beller-Mckenna

Faculty Publications

For most of his adult life, John Lennon fought to define himself against his public identity as "Beatle-:John." That image, crafted by the Beatles' manager Brian Epstein and the pop music industry became a burden to all four of the Beatles during the group's seven years of international star-dom. In place of the rough leather-clad rock and roll band that played rambling one hour sets in the Hamburg Kaiserkeller and the Liverpool Cavern Club during 1960-61, Epstein forged a more commercially viable group of four well (and uniformly) dressed young men whose stage act was reduced to twenty minute carefully …

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Comparative Proteome Analysis Of Brucella Melitensis Vaccine Strain Rev 1 And A Virulent Strain, 16m, Michel Eschenbrenner, Mary Ann Wagner, Troy A. Horn, Jo Ann Kraycer, Cesar V. Mujer, Sue Hagius, Philip Elzer, Vito G. Delvecchio Sep 2002

Comparative Proteome Analysis Of Brucella Melitensis Vaccine Strain Rev 1 And A Virulent Strain, 16m, Michel Eschenbrenner, Mary Ann Wagner, Troy A. Horn, Jo Ann Kraycer, Cesar V. Mujer, Sue Hagius, Philip Elzer, Vito G. Delvecchio

Faculty Publications

The genus Brucella consists of bacterial pathogens that cause brucellosis, a major zoonotic disease characterized by undulant fever and neurological disorders in humans. Among the different Brucella species, Brucella melitensis is considered the most virulent. Despite successful use in animals, the vaccine strains remain infectious for humans. To understand the mechanism of virulence in B. melitensis, the proteome of vaccine strain Rev 1 was analyzed by two-dimensional gel electrophoresis and compared to that of virulent strain 16M. The two strains were grown under identical laboratory conditions. Computer-assisted analysis of the two B. melitensis proteomes revealed proteins expressed in either 16M …

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Alteration Of Oriented Deposition Of Cellulose Microfibrils By Mutation Of A Katanin-Like Microtubule-Severing Protein, David H. Burk, Zheng Hua Ye Sep 2002

Alteration Of Oriented Deposition Of Cellulose Microfibrils By Mutation Of A Katanin-Like Microtubule-Severing Protein, David H. Burk, Zheng Hua Ye

Faculty Publications

It has long been hypothesized that cortical microtubules (MTs) control the orientation of cellulose microfibril deposition, but no mutants with alterations of MT orientation have been shown to affect this process. We have shown previously that in Arabidopsis, the fra2 mutation causes aberrant cortical MT orientation and reduced cell elongation, and the gene responsible for the fra2 mutation encodes a katanin-like protein. In this study, using field emission scanning electron microscopy, we found that the fra2 mutation altered the normal orientation of cellulose microfibrils in walls of expanding cells. Although cellulose microfibrils in walls of wild-type cells were oriented transversely …

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Inhibition Of Amyloid-Β Aggregation And Caspase-3 Activation By The Ginkgo Biloba Extract Egb761, Yuan Luo, Julie V. Smith, Vijaykumar Paramasivam, Adam Burdick, Kenneth J. Curry, Justin P. Buford, Ikhlas Khan, William J. Netzer, Huaxi Xu, Peter Butko Sep 2002

Inhibition Of Amyloid-Β Aggregation And Caspase-3 Activation By The Ginkgo Biloba Extract Egb761, Yuan Luo, Julie V. Smith, Vijaykumar Paramasivam, Adam Burdick, Kenneth J. Curry, Justin P. Buford, Ikhlas Khan, William J. Netzer, Huaxi Xu, Peter Butko

Faculty Publications

Standardized extract from the leaves of the Ginkgo biloba tree, labeled EGb761, has been used in clinical trials for its beneficial effects on brain functions, particularly in connection with age-related dementias and Alzheimer's disease (AD). Substantial experimental evidence indicates that EGb761 protects against neuronal damage from a variety of insults, but its cellular and molecular mechanisms remain unknown. Using a neuroblastoma cell line stably expressing an AD-associated double mutation, we report that EGb761 inhibits formation of amyloid-β (Aβ) fibrils, which are the diagnostic, and possibly causative, feature of AD. The decreased Aβ fibrillogenesis in the presence of EGb761 was observed …

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Metabotropic Glutamate Receptor 5 And Calcium Signaling In Retinal Amacrine Cells, Romina Sosa, Brian Hoffpauir, Michele L. Rankin, Richard C. Bruch, Evanna L. Gleason Aug 2002

Metabotropic Glutamate Receptor 5 And Calcium Signaling In Retinal Amacrine Cells, Romina Sosa, Brian Hoffpauir, Michele L. Rankin, Richard C. Bruch, Evanna L. Gleason

Faculty Publications

To begin to understand the modulatory role of glutamate in the inner retina, we examined the mechanisms underlying metabotropic glutamate receptor 5 (mGluR5)-dependent Ca2+ elevations in cultured GABAergic amacrine cells. A partial sequence of chicken retinal mGluR5 encompassing intracellular loops 2 and 3 suggests that it can couple to both Gq and Gs. Selective activation of mGluR5 stimulated Ca2+ elevations that varied in waveform from cell to cell. Experiments using high external K+ revealed that the mGluR5-dependent Ca2+ elevations are distinctive in amplitude and time course from those engendered by depolarization. Experiments with a Ca2+-free external solution demonstrated that the …

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A Metadata Approach To Preservation Of Digital Resources: The University Of North Texas Libraries' Experience, Daniel Gelaw Alemneh, Samantha K. Hastings, Cathy Hartman Aug 2002

A Metadata Approach To Preservation Of Digital Resources: The University Of North Texas Libraries' Experience, Daniel Gelaw Alemneh, Samantha K. Hastings, Cathy Hartman

Faculty Publications

Preserving long-term access to digital information resources is one of the key challenges facing libraries and information centers today. The University of North Texas (UNT) Libraries has entered into partnership agreements with federal and state agencies to ensure permanent storage and public access to a variety of government information sources. As digital resource preservation encompasses a wide variety of interrelated activities, the UNT Libraries are taking a phased approach to ensure the long-term access to its digital resources. Formulation of preservation policy and creation of preservation metadata for electronic files and digital collections are among the most important steps. This …

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Element Ratios And Aquatic Food Webs, R. Eugene Turner Aug 2002

Element Ratios And Aquatic Food Webs, R. Eugene Turner

Faculty Publications

Organic matter is the result of concentrating a few non-metals that are relatively rare in the earth’s crust. Most of these essential elements are in a rough proportionality within phylogenetic groupings. Life is thus working against a concentration gradient to extract or accumulate these elements, and this metabolic work is accomplished in interrelated and often subtle ways for many other elements. The physiological requirement to sustain these elemental ratios (commonly discussed in terms of the N∶P ratios, but also C∶N, C∶P, and Si∶N ratios) constrains organization at the cellular, organism, and community level. Humans, as geochemical engineers, significantly influence the …

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May Harvey Rest In Peace: Lakin V. Postal Life And Casualty Company, Robert H. Jerry Ii Jul 2002

May Harvey Rest In Peace: Lakin V. Postal Life And Casualty Company, Robert H. Jerry Ii

Faculty Publications

More than a quarter-century has passed since I entered law school as a first-year student and began what has become a career of reading, among other things, cases. I cannot even guess the number of cases I have read in the ensuing years. Most of them have been fairly ordinary, but many have been wonderful for one reason or another. Because I hope to read at least as many cases during my next twenty-five years (or more) of legal study, I am not yet ready to crown any particular case with the title of "my favorite," "the most significant," or …

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Manufacturing Defects, David G. Owen Jul 2002

Manufacturing Defects, David G. Owen

Faculty Publications

No abstract provided.

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Recent Developments Concerning Environmental Law And Agriculture, Linda A. Malone Jul 2002

Recent Developments Concerning Environmental Law And Agriculture, Linda A. Malone

Faculty Publications

No abstract provided.

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Justice Unconceived: How Posterity Has Rights, Aaron-Andrew P. Bruhl Jul 2002

Justice Unconceived: How Posterity Has Rights, Aaron-Andrew P. Bruhl

Faculty Publications

No abstract provided.

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Ecosystem Decay Of Amazonian Forest Fragments: A 22-Year Investigation, William F. Laurance, Thomas E. Lovejoy, Heraldo L. Vasconcelos, Emilio M. Bruna, Raphael K. Didham, Philip C. Stouffer, Claude Gascon, Richard O. Bierregaard, Susan G. Laurance, Erica Sampaio Jun 2002

Ecosystem Decay Of Amazonian Forest Fragments: A 22-Year Investigation, William F. Laurance, Thomas E. Lovejoy, Heraldo L. Vasconcelos, Emilio M. Bruna, Raphael K. Didham, Philip C. Stouffer, Claude Gascon, Richard O. Bierregaard, Susan G. Laurance, Erica Sampaio

Faculty Publications

We synthesized key findings from the Biological Dynamics of Forest Fragments Project, the world's largest and longest-running experimental study of habitat fragmentation. Although initially designed to assess the influence of fragment area on Amazonian biotas, the project has yielded insights that go far beyond the original scope of the study. Results suggest that edge effects play a key role in fragment dynamics, that the matrix has a major influence on fragment connectivity and functioning, and that many Amazonian species avoid even small (<100-m-wide) clearings. The effects of fragmentation are highly eclectic, altering species richness and abundances, species invasions, forest dynamics, the trophic structure of communities, and a variety of ecological and ecosystem processes. Moreover, forest fragmentation appears to interact synergistically with ecological changes such as hunting, fires, and logging, collectively posing an even greater threat to the rainforest biota.

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Volcanic Eruptions On Mid-Ocean Ridges: New Evidence From The Superfast Spreading East Pacific Rise, 17˚-19˚S, John Sinton, Eric Bergmanis, Ken Rubin, Rodey Batiza, Tracy K P Gregg, Karl Gronvold, Ken C. Macdonald, Scott M. White Jun 2002

Volcanic Eruptions On Mid-Ocean Ridges: New Evidence From The Superfast Spreading East Pacific Rise, 17˚-19˚S, John Sinton, Eric Bergmanis, Ken Rubin, Rodey Batiza, Tracy K P Gregg, Karl Gronvold, Ken C. Macdonald, Scott M. White

Faculty Publications

Side-scan sonar, submersible observations and sampling of lava flows from the East Pacific Rise, 17_–19_S constrain the character and variability of submarine volcanic eruptions along mid-ocean ridges. Nine separate lava sequences were mapped using relative age and lithological contrasts among recovered samples. Axial lengths activated during eruptive episodes range from _1 to >18 km; individual flow field areas vary from <1 to >19 km2. Estimated erupted volumes range from <1 to >200 _ 106 m3. The largest unit is the chemically uniform Animal Farm lava near 18_370S. The youngest lava is the Aldo-Kihi flow field, 17_240–340S, probably erupted in the early 1990s from a …

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Insect Conservation In America: Status And Perspectives, Janice L. Bossart, Christopher E. Carlton Jun 2002

Insect Conservation In America: Status And Perspectives, Janice L. Bossart, Christopher E. Carlton

Faculty Publications

No abstract provided.

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Predicting Defection, Elmer J. Schaefer May 2002

Predicting Defection, Elmer J. Schaefer

Faculty Publications

No abstract provided.

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The Body In Resurrection: Science And Scripture On The "Spiritual Body" (1 Cor. 15:35-58), Alan G. Padgett Apr 2002

The Body In Resurrection: Science And Scripture On The "Spiritual Body" (1 Cor. 15:35-58), Alan G. Padgett

Faculty Publications

Understanding the science of Paul’s day helps us understand his comments on the resurrected body. Understanding the science of our day helps us proclaim the resurrection coherently to our own world.

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Survey Of Research On Learning Styles, Rita Dunn, Jeffrey Beaudry Phd, Angela Klavas Apr 2002

Survey Of Research On Learning Styles, Rita Dunn, Jeffrey Beaudry Phd, Angela Klavas

Faculty Publications

A number of studies conducted during the last decade have found that students’ achievement increases when teaching methods match their learning styles—biological and developmental characteristics that affect how they learn.

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Ephesians And Stoic Physics, David E. Fredrickson Apr 2002

Ephesians And Stoic Physics, David E. Fredrickson

Faculty Publications

A primer in Stoic physics is required to understand Ephesians’ description of the body of Christ. The result is problematic

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The Uniform Trust Code (2000): Significant Provisions And Policy Issues, David M. English Apr 2002

The Uniform Trust Code (2000): Significant Provisions And Policy Issues, David M. English

Faculty Publications

This Article provides an overview of the UTC, describes how it responds to recent developments in American trust practice, and describes how its enactment would change the trust law prevailing in most American states.

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Master-Slave Rituals Of Power At A Gold Mine In Nineteenth Century Brazil, Matt D. Childs Apr 2002

Master-Slave Rituals Of Power At A Gold Mine In Nineteenth Century Brazil, Matt D. Childs

Faculty Publications

No abstract provided.

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The Sabbath In The First Creation Accounts, Jiri Moskala Apr 2002

The Sabbath In The First Creation Accounts, Jiri Moskala

Faculty Publications

No abstract provided.

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Inauguration Or Day Of Atonement?: A Response To Norman Young's "Old Testament Background To Hebrews 6:19-20 Revisited", Richard M. Davidson Apr 2002

Inauguration Or Day Of Atonement?: A Response To Norman Young's "Old Testament Background To Hebrews 6:19-20 Revisited", Richard M. Davidson

Faculty Publications

No abstract provided.

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Review: The Issue Of Dystocia Expressed When Sires Varying In Percent Bos Indicus Inheritance Are Mated To Bos Taurus Females., F. A. Thrift, D. E. Franke, T. A. Thrift Mar 2002

Review: The Issue Of Dystocia Expressed When Sires Varying In Percent Bos Indicus Inheritance Are Mated To Bos Taurus Females., F. A. Thrift, D. E. Franke, T. A. Thrift

Faculty Publications

This review reveals that dystocia, with associated increased mortality and(or) reduced survival rate, can be a problem when sires varying in percent Bos indicus inheritance are mated to Bos taurus females. The fact that male calves resulting from these matings are gestated longer and have considerably heavier birth BW than heifer calves is a major contributor to this increased dystocia. A small number of studies involving Brahman sires have indicated larger birth BW for late-born calves in spring calving programs. Furthermore, several studies have documented poor reproductive performance of Brahman bulls, which contributes to subsequent delayed birth of their calves. …

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"Do You Have Any Books On..." An Examination Of Public Library Holdings, Cynthia Bassett, John M. Budd Mar 2002

"Do You Have Any Books On..." An Examination Of Public Library Holdings, Cynthia Bassett, John M. Budd

Faculty Publications

Public librarians have long debated the question of whether quality or popularity should drive collection decisions. A survey of the selected holdings of twenty medium-sized public libraries indicates that both quality and popularity may be determining factors in whether to acquire particular titles. Furthermore, the study suggests that the existence and content of reviews may also influence the likelihood of libraries to select particular titles, as may the selectors' perception of the diversity of their community.

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Two Splice Variants Of Nopp140 In Drosophila Melanogaster, John M. Waggener, Patrick J. Dimario Feb 2002

Two Splice Variants Of Nopp140 In Drosophila Melanogaster, John M. Waggener, Patrick J. Dimario

Faculty Publications

The Nopp140 gene of Drosophila maps within 79A5 of chromosome 3. Alternative splicing yields two variants. DmNopp140 (654 residues) is the sequence homolog of vertebrate Nopp140. Its carboxy terminus is 64% identical to that of the prototypical rat Noppl40. DmNopp140-RGG (688 residues) is identical to DmNopp140 throughout its first 551 residues, but its carboxy terminus contains a glycine/arginine-rich domain that is often found in RNA-binding proteins such as vertebrate nucleolin. Both Drosophila variants localize to nucleoli in Drosophila Schneider II cells and Xenopus oocytes, specifically within the dense fibrillar components. In HeLa cells, DmNopp140-RGG localizes to intact nucleoli, whereas DmNopp140 …

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