Digital Commons Network^™

Rules For Radicals: A Politics Of Patent Law, Kali Murray

Faculty Publications

No abstract provided.

Liberty Of Ecological Conscience, Aaron Lercher

Faculty Publications

Our concern for nonhuman nature can be justified in terms of a human right to liberty of ecological conscience. This right is analogous to the right to religious liberty, and is equally worthy of recognition as that fundamental liberty. The liberty of ecological conscience, like religious liberty, is a negative right against interference. Each ecological conscience supports a claim to protection of the parts of nonhuman nature that are current or potential sites of its active pursuit of natural value. If we acknowledge the fallibility of each conscience in its pursuit of genuine natural value, a policy of indefinitely extensive …

Constitutional Referendum In The United States Of America, William B. Fisch

Faculty Publications

The United States of America, as a federation of now 50 states each with its own constitution and legal system still enjoying a large degree of governmental autonomy within the national legal framework, presents a strikingly mixed picture regarding the use of direct democracy--the submission of proposed governmental action to a popular vote--in law- and constitution-making processes. At the national level, direct democracy has never been used for either type of enactment. At the state and local level, however, its use dates back to colonial times and has been increasing gradually (though still not universal) ever since. Since the mid-19th …

Effects Of Long-Term Hypoxia On Enzymes Of Carbohydrate Metabolism In The Gulf Killifish, Fundulus Grandis, Mery L. Mártinez, Christie Landry, Ryan Boehm, Steve Manning, Bernard B. Rees

Faculty Publications

The goal of the current study was to generate a comprehensive, multi-tissue perspective of the effects of chronic hypoxic exposure on carbohydrate metabolism in the Gulf killifish Fundulus grandis. Fish were held at approximately 1.3 mg l(-1) dissolved oxygen (similar to 3.6 kPa) for 4 weeks, after which maximal activities were measured for all glycolytic enzymes in four tissues (white skeletal muscle, liver, heart and brain), as well as for enzymes of glycogen metabolism (in muscle and liver) and gluconeogenesis (in liver). The specific activities of enzymes of glycolysis and glycogen metabolism were strongly suppressed by hypoxia in white skeletal …

Recent Developments In Copyright Law: Selected U.S. Supreme Court, Court Of Appeals, And District Court Opinions Between February 1, 2005 And May 1, 2006, Tyler T. Ochoa

Faculty Publications

This article highlights nine U.S. copyright law decisions handed down between February 1, 2005 and May 1, 2006. This review was originally delivered as a speech at The 50th Annual Conference of Developments in Intellectual Property Law held by The John Marshall Law School Center for Intellectual Property Law on May 26, 2006. Discussing a wide range of cases from peer-to-peer file sharing to standing, the analysis focuses on the most recent developments in copyright.

Sanctuary Principles For The Successful Church Community, Roy Gane

Faculty Publications

No abstract provided.

Slavery, Prophecy And The American Nation As Seen By The Adventist Pioneers, 1854-1865, Trevor O'Reggio

Faculty Publications

No abstract provided.

Lower Incisors Of Prairie Dogs (Cynomys) As Biorecorders Of Hibernation And Season Of Death, H. Thomas Goodwin, Eva M. Ryckman

Faculty Publications

Incremental dentin and associated enamel, features visible at the surface of lower incisors of rodents, may chronicle important life-history information. This study investigated surface features of lower incisors representing 4 taxa of prairie dogs (Cynomys) in relation to hibernation and season of year. A set of abnormalities in dentin and enamel, observed on 20 of 138 incisors, always chronicled an event ending in late winter or early spring and was interpreted as a hibernation mark. Hibernation was recorded in the incisors of obligate hibernators, C. leucurus and C. gunnisoni, as well as in 20% of specimens of the facultative heterotherm, …

A Fourier Transformation Based Method To Mine Peptide Space For Antimicrobial Activity, Vijayaraj Nagarajan, Navodit Kaushik, Beddhu Murali, Chaoyang Zhang, Sanyogita Lakhera, Mohamed O. Elasri, Youping Deng

Faculty Publications

Background

Naturally occurring antimicrobial peptides are currently being explored as potential candidate peptide drugs. Since antimicrobial peptides are part of the innate immune system of every living organism, it is possible to discover new candidate peptides using the available genomic and proteomic data. High throughput computational techniques could also be used to virtually scan the entire peptide space for discovering out new candidate antimicrobial peptides.

Result

We have identified a unique indexing method based on biologically distinct characteristic features of known antimicrobial peptides. Analysis of the entries in the antimicrobial peptide databases, based on our indexing method, using Fourier transformation …

Post-Thaw Amendment Of Cryopreserved Sperm For Use In Artificial Insemination Of A Viviparous Fish, The Green Swordtail Xiphophorus Helleri, Qiaoxiang Dong, Changjiang Huang, Terrence R. Tiersch

Faculty Publications

Sperm cryopreservation protocols have been developed for live-bearers such as the green swordtail Xiphophorus helleri and the platyfish Xiphophorus couchianus. Despite the high post-thaw motility (∼ 75%) obtained in both species, the requirements of sperm storage within the female reproductive tract coupled with the process of internal fertilization place functional demands upon cryopreserved sperm samples far beyond those of oviparous species. The purpose of this study was to facilitate the artificial insemination process with cryopreserved sperm of X. helleri through evaluation of parameters related to sperm quality after thawing. Specifically, this study evaluated the effects on motility for fresh and …

Tc-99m Pyrophosphate Imaging Of Poloxamer-Treated Electroporated Skeletal Muscle In An In Vivo Rat Model, Kenneth L. Matthews, John N. Aarsvold, Robert A. Mintzer, Chin Tu Chen, Raphael C. Lee

Faculty Publications

Objective: This study investigates whether 99mTc pyrophosphate (PYP) imaging provides a quantitative non-invasive assessment of the extent of electroporation injury, and of the effect of poloxamer in vivo on electroporated skeletal muscle. Methods: High-voltage electrical shock was used to produce electroporation injury in an anesthetized rat's hind limb. In each experiment, the injured limb was treated intravenously by either poloxamer-188, dextran, or saline, and subsequently imaged with 99mTc PYP. The radiotracer's temporal behavior among the experimental groups was compared using curve fitting of time-activity curves from the dynamic image data. Results: The washout kinetics of 99mTc PYP changed in proportion …

Tc-99m Pyrophosphate Imaging Of Poloxamer-Treated Electroporated Skeletal Muscle In An In Vivo Rat Model, Kenneth L. Matthews, John N. Aarsvold, Robert A. Mintzer, Chin Tu Chen, Raphael C. Lee

Faculty Publications

Objective: This study investigates whether 99mTc pyrophosphate (PYP) imaging provides a quantitative non-invasive assessment of the extent of electroporation injury, and of the effect of poloxamer in vivo on electroporated skeletal muscle. Methods: High-voltage electrical shock was used to produce electroporation injury in an anesthetized rat's hind limb. In each experiment, the injured limb was treated intravenously by either poloxamer-188, dextran, or saline, and subsequently imaged with 99mTc PYP. The radiotracer's temporal behavior among the experimental groups was compared using curve fitting of time-activity curves from the dynamic image data. Results: The washout kinetics of 99mTc PYP changed in proportion …

Effects Of Resistant Starch, A Non-Digestible Fermentable Fiber, On Reducing Body Fat, Michael J. Keenan, Jun Zhou, Kathleen L. Mccutcheon, Anne M. Raggio, H. Gale Bateman, Emily Todd, Christina K. Jones, Richard T. Tulley, Sheri Melton, Roy J. Martin, Maren Hegsted

Faculty Publications

Objective: To assess the effects of energy dilution with non-fermentable and fermentable fibers on abdominal fat and gut peptide YY (PYY) and glucagon-like peptide (GLP)-1 expressions, three rat studies were conducted to: determine the effects of energy dilution with a non-ferment-able fiber, compare similar fiber levels of fermentable and non-fermentable fibers, and compare similar metabolizable energy dilutions with fermentable and non-fermentable fibers. Research Methods and Procedures: In Study 1, rats were fed one of three diets with different metabolizable energy densities. In Study 2, rats were fed diets with similar fiber levels using high amylose-resistant cornstarch (RS) or methylcellulose. In …

Introduction: Self-Organization In Nonequilibrium Chemical Systems, Irving R. Epstein, John A. Pojman, Oliver Steinbock

Faculty Publications

The field of self-organization in nonequilibrium chemical systems comprises the study of dynamical phenomena in chemically reacting systems far from equilibrium. Systematic exploration of this area began with investigations of the temporal behavior of the Belousov-Zhabotinsky oscillating reaction, discovered accidentally in the former Soviet Union in the 1950s. The field soon advanced into chemical waves in excitable media and propagating fronts. With the systematic design of oscillating reactions in the 1980s and the discovery of Turing patterns in the 1990s, the scope of these studies expanded dramatically. The articles in this Focus Issue provide an overview of the development and …

Intestinal Enzymes During Malnutrition & Infection In Rabbits, Sufia Islam, Amal K. Mitra, Ashish K. Chowdhury, Nur Haque Alam

Faculty Publications

Background & objectives: Malnutrition plays an important role in the intestinal absorption of nutrients. However, reports are not consistent whether intestinal enzymes are decreased in the presence of malnutrition. It is also not clear whether simultaneous presence of malnutrition and infection adds to the problem of malabsorption of nutrients. The aim of the present study was to determine intestinal functions in terms of concentrations of disaccharidase enzymes during diarrhoea and protein energy malnutrition. Methods: Concentrations of three disaccharidase enzymes, namely maltase, sucrase and lactase were measured in nine energy-restricted and five control rabbits during diarrhoea induced by rabbit diarrhoeagenic Escherichia …

Co-Variation In Soil Biodiversity And Biogeochemistry In Northern And Southern Victoria Land, Antarctica, Byron J. Adams, J. E. Barrett, Ross A. Virginia, Diana H. Wall, S. C. Cary, A. L. Hacker, J. M. Aislabie

Faculty Publications

Data from six sites in Victoria Land (72-77degrees S) investigating co-variation in soil communities (microbial and invertebrate) with biogeochemical properties show the influence of soil properties on habitat suitability varied among local landscapes as well as across climate gradients. Species richness of metazoan invertebrates (Nematoda, Tardigrada and Rotifera) was similar to previous descriptions in this region, though identification of three cryptic nematode species of Eudorylaimus through DNA analysis contributed to the understanding of controls over habitat preferences for individual species. Denaturing Gradient Gel Electrophoresis profiles revealed unexpectedly high diversity of bacteria. Distribution of distinct bacterial communities was associated with specific …

Hiv-1 Dna/Mva Vaccination Reduces The Per Exposure Probability Of Infection During Repeated Mucosal Shiv Challenges, Dennis Ellenberger, Ronald A. Otten, Bin Li, Michael Aidoo, I. Vanessa Rodriguez, Carlos A. Sariol, Melween Martinez, Michael Monsour, Linda Wyatt, Michael G. Hudgens, Edmundo Kraiselburd, Bernard Moss, Harriet Robinson, Thomas Folks, Salvatore Butera

Faculty Publications

Historically, HIV vaccines specifically designed to raise cellular immunity resulted in protection from disease progression but not infection when tested in monkeys challenged with a single high virus exposure. An alternative approach, more analogous to human sexual exposures, is to repetitively challenge immunized monkeys with a much lower dose of virus until systemic infection is documented. Using these conditions to mimic human sexual transmission, we found that a multi-protein DNA/MVA HIV-1 vaccine is indeed capable of protecting rhesus monkeys against systemic infection when repeatedly challenged with a highly heterologous immunodeficiency virus (SHIV). Furthermore, this repetitive challenge approach allowed us to …

Stereoselectivity Of 8-Oh-Dpat Toward The Serotonin 5-Ht1a Receptor: Biochemical And Molecular Modeling Study, Joanna Dabrowska, Michal Brylinski

Faculty Publications

The great majority of pharmacological investigations of 5-HT1A receptors' reactivity has been performed using racemic 8-OH-DPAT, therefore the biochemical as well as behavioral profiles of both 8-OH-DPAT enantiomers are not circumstantiated. In the biochemical study capability of racemic 8-OH-DPAT (0.05, 0.1 mg/kg s.c.) and its counterparts R-8-OH-DPAT (0.05, 0.1 mg/kg s.c.) and S-8-OH-DPAT (0.05, 0.1 mg/kg s.c.) to influence 5-HT synthesis rate in rats' prefrontal cortex, hypothalamus, hippocampus and brainstem was evaluated by HPLC/ED technique. Biochemical results are supported by the exhaustive computational study of possible differences between R- and S-enantiomer toward the 5-HT1A receptor. A reliable 3D model of …

Exposure Of Neonates To Respiratory Syncytial Virus Is Critical In Determining Subsequent Airway Response In Adults, Dahui You, David Becnel, Kai Wang, Michael Ripple, Melissa Daly, Stephania A. Cormier

Faculty Publications

Background: Respiratory syncytial virus (RSV) is the most common cause of acute bronchiolitis in infants and the elderly. Furthermore, epidemiological data suggest that RSV infection during infancy is a potent trigger of subsequent wheeze and asthma development. However, the mechanism by which RSV contributes to asthma is complex and remains largely unknown. A recent study indicates that the age of initial RSV infection is a key factor in determining airway response to RSV rechallenge. We hypothesized that severe RSV infection during neonatal development significantly alters lung structure and the pulmonary immune micro-environment; and thus, neonatal RSV infection is crucial in …

Parasites In A Biodiversity Hotspot: A Survey Of Hematozoa And A Molecular Phylogenetic Analysis Of Plasmodium In New Guinea Skinks, Christopher C. Austin, Susan L. Perkins

Faculty Publications

A sample of 204 skinks (Squamata: Scincidae) from 10 genera representing 24 species were collected from 10 different localities in New Guinea and examined for blood parasites. Hemogregarines, trypanosomes, microfilarial worms, and 8 infections showing 2 distinct morphological types of malaria parasites (Plasmodium sp.) were observed. Molecular sequence data, in the form of mitochondrial cytochrome b sequences from the Plasmodium infections, showed 2 distinct clades of parasites, 1 in Sphenomorphus jobiense hosts and 1 in Emoia spp., which correspond to the 2 morphotypes. There was substantial genetic variation between the 2 clades, as well as within the clade of Emoia …

Rickettsia Felis From Cat Fleas: Isolation And Culture In A Tick-Derived Cell Line, Walairat Pornwiroon, Susan S. Pourciau, Lane D. Foil, Kevin R. Macaluso

Faculty Publications

Rickettsia felis, the etiologic agent of spotted fever, is maintained in cat fleas by vertical transmission and resembles other tick-borne spotted fever group rickettsiae. In the present study, we utilized an Ixodes scapularis-derived tick cell line, ISE6, to achieve isolation and propagation of R. felis. A cytopathic effect of increased vacuolization was commonly observed in R. felis-infected cells, while lysis of host cells was not evident despite large numbers of rickettsiae. Electron microscopy identified rickettsia-like organisms in ISE6 cells, and sequence analyses of portions of the citrate synthase (gltA), 16S rRNA, Rickettsia genus-specific 17-kDa antigen, and spotted fever group-specific outer …

The Drosophila Boundary Element-Associated Factors Beaf-32a And Beaf-32b Affect Chromatin Structure, Matthew K. Gilbert, Yian Yee Tan, Craig M. Hart

Faculty Publications

Binding sites for the Drosophila boundary element-associated factors BEAF-32A and -32B are required for the insulator activity of the scs' insulator. BEAF binds to hundreds of sites on polytene chromosomes, indicating that BEAF-utilizing insulators are an important class in Drosophila. To gain insight into the role of BEAF in flies, we designed a transgene encoding a dominant-negative form of BEAF under GAL4 UAS control. This BID protein encompasses the BEAF self-interaction domain. Evidence is provided that BID interacts with BEAF and interferes with scs' insulator activity and that BEAF is the major target of BID in vivo. BID expression during …

Health In Cuba, Richard S. Cooper, Joan F. Kennelly, Pedro Orduñez-Garcia

Faculty Publications

The poorer countries of the world continue to struggle with an enormous health burden from diseases that we have long had the capacity to eliminate. Similarly, the health systems of some countries, rich and poor alike, are fragmented and inefficient, leaving many population groups underserved and often without health care access entirely. Cuba represents an important alternative example where modest infrastructure investments combined with a well-developed public health strategy have generated health status measures comparable with those of industrialized countries. Areas of success include control of infectious diseases, reduction in infant mortality, establishment of a research and biotechnology industry, and …

Classical Biological Control Of The Papaya Mealybug, Paracoccus Marginatus (Hemiptera: Pseudococcidae) In The Republic Of Palau, R. Muniappan, D. E. Meyerdirk, F. M. Sengebau, D. D. Berringer, G. V.P. Reddy

Faculty Publications

The papaya mealybug (PM), Paracoccus marginatus Williams and Granara de Willink (Hemiptera: Pseudococcidae), a pest in Central America and the Caribbean, was noted to have established on Palau in March 2003 and was causing serious damage to papaya, plumeria, hibiscus, and other plants. The parasitoids Anagyrus loecki Noyes, Pseudleptomastix mexicana Noyes and Schauff, and Acerophagus papayae Noyes and Schauff (Hymenoptera: Encyrtidae) totaling 24,586 were imported from Puerto Rico and field released in Palau from August 2003 to June 2004. Anagyrus loecki and A. papayae appear to be promising biological control agents of PM in Palau. No field recovery of P. …

The Importance Of Demographic Niches To Tree Diversity, Richard Condit, Peter Ashton, Sarayudh Bunyavejchewin, H. S. Dattaraja, Stuart Davies, Shameema Esufali, Corneille Ewango, Robin Foster, I. A.U.N. Gunatilleke, C. V.S. Gunatilleke, Pamela Hall, Kyle E. Harms, Terese Hart, Consuelo Hernandez, Stephen Hubbell, Akira Itoh, Somboon Kiratiprayoon, James Lafrankie, Suzanne Loo De Lao, Jean Remy Makana, Md Nur Supardi Noor, Abdul Rahman Kassim, Sabrina Russo, Raman Sukumar, Cristián Samper, Hebbalalu S. Suresh, Sylvester Tan, Sean Thomas, Renato Valencia, Martha Vallejo, Gorky Villa, Tommaso Zillio

Faculty Publications

Most ecological hypotheses about species coexistence hinge on species differences, but quantifying trait differences across species in diverse communities is often unfeasible. We examined the variation of demographic traits using a global tropical forest data set covering 4500 species in 10 large-scale tree inventories. With a hierarchical Bayesian approach, we quantified the distribution Of mortality and growth rates of all tree species at each site. This allowed us to test the prediction that demographic differences facilitate species richness, as suggested by the theory that a tradeoff between high growth and high survival allows species to coexist. Contrary to the prediction, …

Ecological Fitting As A Determinant Of The Community Structure Of Platyhelminth Parasites Of Anurans, Daniel R. Brooks, Virginia León-Règagnon, Deborah Mclennan, Derek Zelmer

Faculty Publications

Host–parasite associations are assumed to be ecologically specialized, tightly coevolved systems driven by mutual modification in which host switching is a rare phenomenon. Ecological fitting, however, increases the probability of host switching, creating incongruences between host and parasite phylogenies, when (1) specialization on a particular host resource is a shared characteristic of distantly related parasites, and (2) the resource being tracked by the parasite is widespread among many host species. We investigated the effect of ecological fitting on structuring the platyhelminth communities of anurans from a temperate forest and grassland in the United States and tropical dry and wet forests …

Biocatalytic And Antimetastatic Studies Of The Marine Cembranoids Sarcophine And 2-Epi-16-Deoxysarcophine, Swapnali S. Sawant, Diaa T.A. Youssef, Jane Reiland, Melissa Ferniz, Dario Marchetti, Khalid A. El Sayed

Faculty Publications

The soft coral Sarcophyton glaucum is a rich resource of several bioactive cembranoids. Sarcophytol A (1) and sarcophine (2) are cembranoid diterpenes reported from this soft coral and extensively investigated for their cancer chemopreventive properties. This study aimed at investigating the antimetastatic potential of the major cembranoids, sarcophine (2) and 2-epi-16-deoxysarcophine (3), from the Red Sea soft coral S. glaucum. Biocatalytic transformation of 3 using Rhizopus stolonifer ATCC 6227a and Absidia spinosa ATCC 6648 afforded four new metabolites, 5-7 and 9, along with the known 9α-hydroxysarcophine (8). Sarcophine, 2-epi-16-deoxysarcophine, and metabolites 5-9 revealed significant antimetastatic activity against the highly metastatic …

Reca And Rada Proteins Of Brucella Abortus Do Not Perform Overlapping Protective Dna Repair Functions Following Oxidative Burst, Christelle M. Roux, Natha J. Booth, Bryan H. Bellaire, Jason M. Gee, R. Martin Roop, Michael E. Kovach, Renée M. Tsolis, Philip H. Elzer, Don G. Ennis

Faculty Publications

Very little is known about the role of DNA repair networks in Brucella abortus and its role in pathogenesis. We investigated the roles of RecA protein, DNA repair, and SOS regulation in B. abortus. While recA mutants in most bacterial species are hypersensitive to UV damage, surprisingly a B. abortus recA null mutant conferred only modest sensitivity. We considered the presence of a second RecA protein to account for this modest UV sensitivity. Analyses of the Brucella spp. genomes and our molecular studies documented the presence of only one recA gene, suggesting a RecA-independent repair process. Searches of the available …

Species-Habitat Associations In A Sri Lankan Dipterocarp Forest, C. V.S. Gunatilleke, I. A.U.N. Gunatilleke, S. Esufali, K. E. Harms, P. M.S. Ashton, D. F.R.P. Burslem, P. S. Ashton

Faculty Publications

Forest structure and species distribution patterns were examined among eight topographically defined habitats for the 205 species with stems ≥ 1 cm dbh inhabiting a 25-ha plot in the Sinharaja rain forest, Sri Lanka. The habitats were steep spurs, less-steep spurs, steep gullies and less-steep gullies, all at either lower or upper elevations. Mean stem density was significantly greater on the upper spurs than in the lower, less-steep gullies. Stem density was also higher on spurs than in gullies within each elevation category and in each upper-elevation habitat than in its corresponding lower-elevation habitat. Basal area varied less among habitats, …

Stat5 Activators Modulate Acyl Coa Oxidase (Aox) Expression In Adipocytes And Stat5a Binds To The Aox Promoter In Vitro, Ann A. Coulter, Jacqueline M. Stephens

Faculty Publications

Growth hormone (GH) diminishes adipose tissue mass in vivo and prolactin (PRL) can also modulate adipocyte metabolism. Both GH and PRL are potent activators of STAT5 and exert a variety of effects on adipocyte gene expression. In this study, we have demonstrated that GH and PRL increase the mRNA of acyl CoA oxidase in 3T3-L1 adipocytes. We also identified seven putative STAT elements in the murine AOX promoter. We observed that GH modulates protein binding to the majority of these promoter elements. However, GH induced very potent binding to -1841 to -1825 of the murine AOX promoter. EMSA supershift analysis …