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Host Specificity Tests Of Gratiana Graminea (Coleoptera: Chrysomelidae), A Potential Biological Control Agent Of Tropical Soda Apple, Solanum Viarum (Solanaceae), J. Medal, N. Bustamante, M. Vitorino, L. Beal, W. Overholt, R. Diaz, J. Cuda

Faculty Publications

Multiple-choice and no-choice tests were conducted at the Department of Agriculture, Division of Plant Industry Quarantine facility in Gainesville, FL to determine the specificity of the Brazilian leaf-beetle Gratiana graminea Klug, a candidate for biological control of Solanum viarum, tropical soda apple. One hundred fifteen plant species in 32 families were included in the feeding-oviposition multiple-choice tests including the target weed and the 5 major cultivated Solanaceae Capsicum annuum L., Lycopersicon sculentum Mill., Nicotiana tabacum L., Solanum melongena L., and Solanum tuberosum L. Eight to 12 plant species, including always the main target weed, growing in 1-gallon pots were simultaneously …

Historical Divergence And Gene Flow: Coalescent Analyses Of Mitochondrial, Autosomal And Sex-Linked Loci In Passerina Buntings, Matthew D. Carling, Irby J. Lovette, Robb T. Brumfield

Faculty Publications

Quantifying the role of gene flow during the divergence of closely related species is crucial to understanding the process of speciation. We collected DNA sequence data from 20 loci (one mitochondrial, 13 autosomal, and six sex-linked) for population samples of Lazuli Buntings (Passerina amoena) and Indigo Buntings (Passerina cyanea) (Aves: Cardinalidae) to test explicitly between a strict allopatric speciation model and a model in which divergence occurred despite postdivergence gene flow. Likelihood ratio tests of coalescent-based population genetic parameter estimates indicated a strong signal of postdivergence gene flow and a strict allopatric speciation model was rejected. Analyses of partitioned datasets …

Determination Of Sperm Concentration For Small-Bodied Biomedical Model Fishes By Use Of Microspectrophotometry, Ereene Tan, Huiping Yang, Terrence R. Tiersch

Faculty Publications

The goal of this study was to establish an efficient method for determination of sperm concentration requiring only 1-2 μL of sample by use of microspectrophotometry. The objectives were (1) determination of wavelengths with absorbance profiles appropriate for analysis of sperm suspensions from zebrafish Danio rerio, green swordtail Xiphophorus helleri, and medaka Oryzias latipes collected by crushing of dissected testis or by stripping of live males; (2) generation of standard curves and equations between sperm sample absorbance and sperm concentration estimated by hemocytometer counts; (3) accuracy verification of equations for estimating concentration by microspectrophotometry; and (4) analysis of the precision …

Evaluating Ecosystem Response To Oyster Restoration And Nutrient Load Reduction With A Multispecies Bioenergetics Model, Richard S. Fulford, Denise L. Breitburg, Mark Luckenbach, Roger I.E. Newell

Faculty Publications

Many of the world's coastal ecosystems are impacted by multiple stressors each of which may be subject to different management strategies that may have overlapping or even conflicting objectives. Consequently, management results may be indirect and difficult to predict or observe. We developed a network simulation model intended specifically to examine ecosystem-level responses to management and applied this model to a comparison of nutrient load reduction and restoration of highly reduced stocks of bivalve suspension feeders (eastern oyster, Crassostrea virginica) in an estuarine ecosystem (Chesapeake Bay, USA). Model results suggest that a 50% reduction in nutrient inputs from the watershed …

Natural Selection On Testosterone Production In A Wild Songbird Population, Joel W. Mcglothlin, Danielle J. Whittaker, Sara E. Schrock, Nicole M. Gerlach, Jodie M. Jawor, Eric A. Snajdr, Ellen D. Ketterson

Faculty Publications

Because of their role in mediating life-history trade-offs, hormones are expected to be strongly associated with components of fitness; however, few studies have examined how natural selection acts on hormonal variation in the wild. In a songbird, the dark-eyed junco (Junco hyemalis), field experiments have shown that exogenous testosterone alters individuals' resolution of the survival-reproduction trade-off, enhancing reproduction at the expense of survival. Here we used standardized injections of gonadotropin-releasing hormone (GnRH) to assay variation in the testosterone production of males. Using measurements of annual survival and reproduction, we found evidence of strong natural selection acting on GnRH-induced …

Hispanic Computer Brigade Silicon Valley Computer Camp, Patricia Backer, Belle Wei

Faculty Publications

Hispanic students are vastly underrepresented in computing during a time when the Hispanic population in the U.S. is growing dramatically. By 2020, Hispanics will account for 50% of the workers in California. And, by 2050, the Hispanic population is projected to triple in the United States with 25% of the U.S. population being of Hispanic origin. The College of Engineering (CoE) at San José State University proposed a new approach for recruiting Hispanic students into computing disciplines and careers through the Hispanic Computer Brigade (HCB) initiative. By forming HCBs in two local high schools, we aimed to inspire and engage …

Intravenous Ibuprofen: The First Injectable Product For The Treatment Of Pain And Fever, P Brandon Bookstaver, April D. Miller, Celeste N. Rudisill, Leann B. Norris

Faculty Publications

This paper reviews the current data on the use of the first approved intravenous ibuprofen product for the management of post-operative pain and fever in the United States. The management of acute and post-operative pain and fever with nonsteroidal anti-inflammatory agents (NSAIDs) is well documented. A search in Medline and International Pharmaceutical Abstracts of articles until the end of November 2009 and references of all citations were conducted. Available manufacturer data on file were also analyzed for this report. Several randomized controlled studies have demonstrated the opioid-sparing and analgesic effects of 400 and 800 mg doses of intravenous ibuprofen in …

High Amino Acid Diversity And Positive Selection At A Putative Coral Immunity Gene (Tachylectin-2), Marshall L. Hayes, Ron I. Eytan, Michael E. Hellberg

Faculty Publications

Background. Genes involved in immune functions, including pathogen recognition and the activation of innate defense pathways, are among the most genetically variable known, and the proteins that they encode are often characterized by high rates of amino acid substitutions, a hallmark of positive selection. The high levels of variation characteristic of immunity genes make them useful tools for conservation genetics. To date, highly variable immunity genes have yet to be found in corals, keystone organisms of the world's most diverse marine ecosystem, the coral reef. Here, we examine variation in and selection on a putative innate immunity gene from Oculina, …

Performance, Properties And Plasticity Of Identified Oxytocin And Vasopressin Neurones In Vitro, William E. Armstrong, L. Wang, C. Li, R. Teruyama

Faculty Publications

The neurohypophysial hormones oxytocin (OT) and vasopressin (VP) originate from hypothalamic neurosecretory cells in the paraventricular and supraoptic (SON) nuclei. The firing rate and pattern of action potentials arising from these neurones determine the timing and quantity of peripheral hormone release. We have used immunochemical identification of biocytin-filled SON neurones in hypothalamic slices in vitro to uncover differences between OT and VP neurones in membrane and synaptic properties, firing patterns, and plasticity during pregnancy and lactation.In this review, we summarise some recent findings from this approach: (i) VP neuronal excitability is influenced by slow (sDAP) and fast (fDAP) depolarising afterpotentials …

Interaction Of Francisella Asiatica With Tilapia (Oreochromis Niloticus) Innate Immunity, Esteban Soto, Denise Fernandez, Ronald Thune, John P. Hawke

Faculty Publications

Members of the genus Francisella are facultative intracellular bacteria that cause important diseases in a wide variety of animals worldwide, including humans and fish. Several genes that are important for intramacrophage survival have been identified, including the iglC gene, which is found in the iglABCD operon in the Francisella sp. pathogenicity island (FPI). In the present study, we examined the interaction of wild-type Francisella asiatica and a ΔiglC mutant strain with fish serum and head kidney-derived macrophages (HKDM). Both the wild-type and the mutant strains were resistant to killing by normal and heat-inactivated sera. The wild-type F. asiatica is able …

Siamese Cooperates With The Cdh1-Like Protein Ccs52a1 To Establish Endoreplication In Arabidopsis Thaliana Trichomes, Remmy Kasili, Jason D. Walker, L. Alice Simmons, Jing Zhou, Lieven De Veylder, John C. Larkin

Faculty Publications

Endoreplication, also known as endoreduplication, is a phyogenetically widespread modified version of the cell cycle in which DNA replication is not followed by cell division. The SIAMESE (SIM) gene of Arabidopsis thaliana encodes the founding member of a novel class of plant-specific cyclin-dependent kinase (CDK) inhibitors and is a key regulator of endoreplication during the development of trichomes (shoot epidermal hairs). Here, we have identified mutations in the CCS52A1 gene as genetic modifiers of the multicellular trichome phenotype of sim mutants. Loss-of-function ccs52A1 mutations dramatically enhance the multicellularity of sim mutants trichomes in double mutants, whereas overexpression of CCS52A1 completely …

Desire In Marble: Vision, Classical Antiquity, And Homoerotic Spectacle In Melville's Travel Writing, David Greven

Faculty Publications

No abstract provided.

Transnational Integration Regimes As Development Programs, Laszlo Bruszt, Gerald A. Mcdermott

Faculty Publications

In drawing on recent advances in international and comparative political economy, this paper argues that diverging paths of institutional development among emerging market democracies are driven by the Transnational Integration Regimes (TIRs), in which a country is embedded.

As development programs, TIRs differ in their effectiveness not simply in terms of their incentives and largess and more in terms of their emphasis on building institutional capacities, empowering a variety of domestic state and non-state actors via multiplex methods of assistance and monitoring, and their ability to merge monitoring and learning at both the national and supra-national levels. We develop a …

The Study Of Teachers’ Task Values And Self-Efficacy On Their Commitment And Effectiveness For Technology-Instruction Integration, Chia-Jung Lin, Mei-Yan Lu

Faculty Publications

The city of Taipei has been considered as a leading role of information technology education in Taiwan. However, many questions have been waited to be answered. The purpose of this study was to investigate the current situations and problems of primary school teachers’ technology-instruction integration. By implementing the approach of cognitive motivators and the human performance technology (HPT) theory, this study also investigated the relationships among teachers’ cognitive motivators (self-efficacy and task values) and their commitment and effort on technology-instruction integration. The researchers delivered 2,952 questionnaires via Internet, e-mail and airmail in January 2008. Finally, 1,549 questionnaires replied back and …

Steroid Receptor Expression In The Fish Inner Earvaries With Sex, Social Status, And Reproductive State, Karen P. Maruska, Russell D. Fernald

Faculty Publications

Background: Gonadal and stress-related steroid hormones are known to influence auditory function across vertebrates but the cellular and molecular mechanisms responsible for steroid-mediated auditory plasticity at the level of the inner ear remain unknown. The presence of steroid receptors in the ear suggests a direct pathway for hormones to act on the peripheral auditory system, but little is known about which receptors are expressed in the ear or whether their expression levels change with internal physiological state or external social cues. We used qRT-PCR to measure mRNA expression levels of multiple steroid receptor subtypes (estrogen receptors: ERα, ERβa, ERβb; androgen …

Transcriptional Factors That Promote Formation Of White Adipose Tissue, Ursula A. White, Jacqueline M. Stephens

Faculty Publications

Adipocytes are highly specialized cells that play a major role in energy homeostasis in vertebrate organisms. Excess adipocyte size or number is a hallmark of obesity, which is currently a global epidemic. Obesity is a major risk factor for the development of type II diabetes (T2DM), cardiovascular disease, and hypertension. Obesity and its related disorders result in dysregulation of the mechanisms that control the expression of metabolic and endocrine related genes in adipocytes. Therefore, understanding adipocyte differentiation is relevant not only for gaining insight into the pathogenesis of metabolic diseases, but also for identifying proteins or pathways which might be …

Food Insecurity As A Barrier To Sustained Antiretroviral Therapy Adherence In Uganda, Sheri D. Weiser, David M. Tuller, Edward A. Frongillo Jr., Jude Senkungu, Nozmu Mukiibi, David R. Bangsberg

Faculty Publications

Background: Food insecurity is emerging as an important barrier to antiretroviral (ARV) adherence in sub-Saharan Africa and elsewhere, but little is known about the mechanisms through which food insecurity leads to ARV non-adherence and treatment interruptions.

Methodology: We conducted in-depth, open-ended interviews with 47 individuals (30 women, 17 men) living with HIV/ AIDS recruited from AIDS treatment programs in Mbarara and Kampala, Uganda to understand how food insecurity interferes with ARV therapy regimens. Interviews were transcribed, coded for key themes, and analyzed using grounded theory.

Findings: Food insecurity was common and an important barrier to accessing medical care and ARV …

Past And Projected Future Changes In Snowpack And Soil Frost At The Hubbard Brook Experimental Forest, New Hampshire, Usa, John L. Cambell, Scott V. Ollinger, Gerald N. Flerchinger, Haley Wicklein, Katharine Hayhoe, Amey S. Bailey

Faculty Publications

Long‐term data from the Hubbard Brook Experimental Forest in New Hampshire show that air temperature has increased by about 1 °C over the last half century. The warmer climate has caused significant declines in snow depth, snow water equivalent and snow cover duration. Paradoxically, it has been suggested that warmer air temperatures may result in colder soils and more soil frost, as warming leads to a reduction in snow cover insulating soils during winter. Hubbard Brook has one of the longest records of direct field measurements of soil frost in the United States. Historical records show no long‐term trends in …

"The Lord Is A God Of Justice" (Isaiah 30:18): The Prophetic Insistence On Justice In Social Context, Rolf A. Jacobson

Faculty Publications

The prophets insisted on economic justice because they understood the Lord to be “a God of justice.” If justice is part of the very character of God, God's people must also embody justice.

To Possess Is Human?: Not In The Economy Of Salvation!, Amy E. Marga

Faculty Publications

A meaningful conversation about faith and economics requires the participants to know the fundamentals of each discipline. Here, Professor Marga offers an introduction to basic principles in the Christian “economy of salvation.”

Biogeographic Breaks In Vanuatu, A Nascent Oceanic Archipelago, Alison M. Hamilton, Elaine R. Klein, Christopher C. Austin

Faculty Publications

The study of distinct biogeographic demarcations has played a pivotal role in our understanding processes responsible for patterns of species distributions and, importantly, the role of geologic processes in promoting biotic diversification. Biogeographic barriers such as Wallace's line have been shown to be the result of old geologic processes shaping ancient faunal or floral diversification events. Based on distributions of birds, bats, reptiles, plants, and invertebrates we identify a distinct biogeographic disjunction in Vanuatu, a geologically nascent oceanic archipelago. We discuss mechanisms contributing to this concordant pattern across these disparate taxonomic groups in light of geologic history, ocean currents, vegetation, …

The Genome Of A Songbird, Wesley C. Warren, David F. Clayton, Hans Ellegren, Arthur P. Arnold, Ladeana W. Hillier, Axel Künstner, Steve Searle, Simon White, Albert J. Vilella, Susan Fairley, Andreas Heger, Lesheng Kong, Chris P. Ponting, Erich D. Jarvis, Claudio V. Mello, Pat Minx, Peter Lovell, Tarciso A.F. Velho, Margaret Ferris, Christopher N. Balakrishnan, Saurabh Sinha, Charles Blatti, Sarah E. London, Yun Li, Ya Chi Lin, Julia George, Jonathan Sweedler, Bruce Southey, Preethi Gunaratne, Michael Watson, Kiwoong Nam, Niclas Backström, Linnea Smeds, Benoit Nabholz

Faculty Publications

The zebra finch is an important model organism in several fields with unique relevance to human neuroscience. Like other songbirds, the zebra finch communicates through learned vocalizations, an ability otherwise documented only in humans and a few other animals and lacking in the chickenthe only bird with a sequenced genome until now. Here we present a structural, functional and comparative analysis of the genome sequence of the zebra finch (Taeniopygia guttata), which is a songbird belonging to the large avian order Passeriformes. We find that the overall structures of the genomes are similar in zebra finch and chicken, but they …

Discovery And Characterization Of Proteins Associated With Aflatoxin-Resistance: Evaluating Their Potential As Breeding Markers, Robert L. Brown, Zhi Yuan Chen, Marilyn Warburton, Meng Luo, Abebe Menkir, Ahmad Fakhoury, Deepak Bhatnagar

Faculty Publications

Host resistance has become a viable approach to eliminating aflatoxin contamination of maize since the discovery of several maize lines with natural resistance. However, to derive commercial benefit from this resistance and develop lines that can aid growers, markers need to be identified to facilitate the transfer of resistance into commercially useful genetic backgrounds without transfer of unwanted traits. To accomplish this, research efforts have focused on the identification of kernel resistanceassociated proteins (RAPs) including the employment of comparative proteomics to investigate closely-related maize lines that vary in aflatoxin accumulation. RAPs have been identified and several further characterized through physiological …

Symbiosis In Subterranean Termites: A Review Of Insights From Molecular Studies, Claudia Husseneder

Faculty Publications

The symbiotic relationship of termites and their eukaryotic and prokaryotic gut microbiota is a focal point of research because of the important roles symbionts play in termite nutrition. The use of molecular methods has recently provided valuable insights into the species diversity and the roles of microorganisms in the guts of termites. This paper provides a review of the current knowledge of symbiont species inventories, genome analysis, and gene expression in the guts of subterranean termites. Particular emphasis is given to the termite genera Reticulitermes and Coptotermes (Isoptera: Rhinotermitidae), because they contain pest species of global impact in their native …

Fpga Acceleration Of The Phylogenetic Likelihood Function For Bayesian Mcmc Inference Methods, Stephanie Zierke, Jason D. Bakos

Faculty Publications

Background
Likelihood (ML)-based phylogenetic inference has become a popular method for estimating the evolutionary relationships among species based on genomic sequence data. This method is used in applications such as RAxML, GARLI, MrBayes, PAML, and PAUP. The Phylogenetic Likelihood Function (PLF) is an important kernel computation for this method. The PLF consists of a loop with no conditional behavior or dependencies between iterations. As such it contains a high potential for exploiting parallelism using micro-architectural techniques. In this paper, we describe a technique for mapping the PLF and supporting logic onto a Field Programmable Gate Array (FPGA)-based co-processor. By leveraging …

Learning, Changing, And Doing: A Model For Transformational Leadership Development In Religious And Non-Profit Organizations, Skip Bell

Faculty Publications

Religious and non-profit organizations seek people who continually experience learning and growth within their work. Such persons will not stop at doing their jobs well; they develop as leaders who in turn create positive transformation within their group. The purpose of this article is to define a transformational leadership development process integrating learning, changing, and doing as the preferred model in the life of a religious or non-profit organization. The outcome will be persons who grow as leaders, experience meaning in their service, and contribute significant organizational change.

Associations Between Polycyclic Aromatic Hydrocarbon-Related Exposures And P53 Mutations In Breast Tumors, Irina Mordukhovich, Pavel Rossner Jr., Mary Beth Terry, Regina M. Santella, Yu-Jing Zhang, Hanina Hibshoosh, Lorenzo Memeo, Mahesh Mansukhani, Chang-Min Long, Gail Garbowski, Meenakshi Agrawal, Mia M. Gaudet, Susan E. Steck, Sharon K. Sagiv, Sybil M. Eng, Susan L. Teitelbaum, Alfred I. Neugut, Kathleen Conway-Dorsey, Marilie D. Gammon

Faculty Publications

BACKGROUND: Previous studies have suggested that polycyclic aromatic hydrocarbons (PAHs) may be associated with breast cancer. However, the carcinogenicity of PAHs on the human breast remains unclear. Certain carcinogens may be associated with specific mutation patterns in the p53 tumor suppressor gene, thereby contributing information about disease etiology.

OBJECTIVES: We hypothesized that associations of PAH-related exposures with breast cancer would differ according to tumor p53 mutation status, effect, type, and number.

METHODS: We examined this possibility in a population-based case–control study using polytomous logistic regression. As previously reported, 151 p53 mutations among 859 tumors were identified using Surveyor nuclease and …

Is The Sabbath Part Of The "New Covenant"?, Roy E. Gane

Faculty Publications

No abstract provided.

Tradition And Innovation: Extremeñan Connections And Collaborations, Jo Farb Hernandez

Faculty Publications

The writer discusses the collaboration between California artist Sam Hernández and the Moreno León family from Extremadura, Spain, who create tinajas, monumental ceramic storage vessels. While Hernández approached this collaboration with admiration for the elegance of the traditional tinaja, and respect for the Moreno León ceramists, the innovation in superimposing his own images led to a totally new set of sculptures that, though linked to his earlier oeuvre, are markedly different from it. By recontextualizing the tinaja into a contemporary narrative through the overlay of his chosen subject matter, he shifted the collaboration into a new territory, as, at the …

Preliminary Results Of Combining Low Frequency Low Intensity Ultrasound And Liposomal Drug Delivery To Treat Tumors In Rats, William G. Pitt, Ghaleb A. Husseini, Beverly L. Roeder, David Dickinson, David Warden, Jonathan Hartley, Peter Jones

Faculty Publications

Ultrasound is a convenient trigger for site-specific drug delivery in cancer therapy. Nanosized liposomes formulated from soy phosphatidyl choline, cholesterol, 1,2-distearoyl-sn-glycero3-phosphoethanolamine-N-[carboxy(polyethylene glycol)-2000] and alpha-tocopherol were loaded with Doxorubicin (Dox)using a pH gradient. The liposomal suspension was infused through the tail vein of BDIX rats possessing bilateral intradermal DHD/K12 tumors on their hind legs. Then 20-kHz ultrasound was applied to only one of the tumors for 15 minutes. This therapy was repeated weekly for 4 weeks. The results showed that in five of sixrats, the tumors regressed to non-measurable size within 4 weeks. A paired comparison of the normalized size of …