Digital Commons Network^™

Stromal Vascular Fraction Restores Vasodilatory Function By Reversing Mitochondrial Dysfunction And Oxidative Stress In Aging-Induced Coronary Microvascular Disease., Evan Paul Tracy

Electronic Theses and Dissertations

Background: Coronary Microvascular Disease (CMD) presents in aging post-menopausal women with chronic angina due to microvascular hyperconstriction. The objective was to identify mechanisms of adipose stromal vascular fraction’s (SVF) restorative effects on vasodilation. We hypothesize aging-induced CMD is caused by a) abrogated flow-mediated dilation (FMD) due to loss of nitric oxide signaling and b) ROS-dependent βADR desensitization & internalization, reversible by ameliorating mitochondrial dysfunction and oxidative stress with SVF. Methods: Coronary microvessels were isolated from female rats either young, old, or old with SVF tail-vein injection (OSVF). Pressure myography, RNA-sequencing, immunofluorescence, Western blotting, and morphological analysis were performed to …

Ecological Differences In The Associations Between Air Pollution, Greenness, And Risk Of Stroke: The Regards Study., Daniel W. Riggs

Electronic Theses and Dissertations

The adverse health effects of air pollution have long been recognized, with the majority of morbidity and mortality due to its effects on the cardiovascular system. Alternatively, living in areas with higher greenness has been found to be beneficial to a wide range of health outcomes. However, few studies have considered that these relationships may vary depending on the surrounding ecosystem. The purpose of this dissertation was to examine the effects of long-term exposure to air pollution and greenness on incidence of stroke, and how these relationships vary with the major ecological regions of the United States. We utilized the …

Bayesian Methodologies For Constrained Spaces., Siddhesh Kulkarni

Electronic Theses and Dissertations

Due to advances in technology, there is a presence of directional data in a wide variety of fields. Often distributions to model directional data are defined on manifold or constrained spaces. Regular statistical methods applied to data defined on special geometries can give misleading results, and this demands new statistical theory. This dissertation addresses two such problems and develops Bayesian methodologies to improve inference in these arenas. It consists of two projects: 1. A Bayesian Methodology for Estimation for Sparse Canonical Correlation, and 2. Bayesian Analysis of Finite Mixture Model for Spherical Data. In principle, it can be challenging to …

Stability Analysis Of Ionically And Electronically Conductors In Energy Applications., Tanner D. Whatley

Electronic Theses and Dissertations

The increasing need of electrical power around the world creates the search for sustainable energy to become a major stride in the manufacturing industry. The pursuit of energy storage systems being better, longer lasting, lighter, and cost effective have been a priority for companies, but one of the most crucial attributes in a battery should be its safety without the reduction in performance. For energy conversion and storage devices, such as solar cells and batteries, many important components are sensitive to air or moisture, therefore, the stability studies are important. This thesis focuses on two representative materials: hydrogenated carbon nanosheets …

Characterization Of Microbotryum Lychnidis-Dioicae Secreted Effector Proteins That Manipulate Its Host Plant, Silene Latifolia., Ming-Chang Tsai

Electronic Theses and Dissertations

The smut fungal species Microbotryum lychnidis-dioicae is an obligate phytopathogen colonizing the plant host, Silene latifolia. A significant feature of M. lychnidis-dioicae infection is that the fungus can replace pollen on the anthers of susceptible host plants with fungal teliospores, thus earning the fungus the name: anther smut disease of flowers. The fungus synthesizes and secretes effector proteins into the cells of the plant host during infection, and the protein-protein interactions may interfere with and modify metabolism, plant development, and gene expression of the host to allow fungal colonization. Two potential fungal effector proteins, MVLG_06175 and MVLG_05122, were identified …

Cultivating The Human Narrative: On Nietzsche, Science Fiction, And The Aesthetics Of Life., Derek Carpenter

Electronic Theses and Dissertations

In this dissertation, I will argue that there is a specific model of morality which can account for the intuition about moral features that we take to be intrinsic to the human type. This model of morality is that of Virtue Ethics, but not a conventional kind of Virtue Ethics. Instead, I will argue that the moral philosophy of Friedrich Nietzsche presents a model of cultivating narrative types that we take to be grounded in the kinds of beings we are, without resorting to any problematic metaphysical systems. This will require that I defend a novel interpretation of Nietzsche’s ethics …

A New Sir Model With Mobility., Ciana Applegate

Electronic Theses and Dissertations

In this paper, a mobility-based SIR model is built to understand the spread of the pandemic. A traditional SIR model used in epidemiology describes the transition of particles among states, such as susceptible, infected, and recovered states. However, the traditional model has no movement of particles. There are many variations of SIR models when it comes to the factor of mobility, the majority of studies use mobility intensity or population density as a measure of mobility. In this paper, a new dynamical SIR model, including the spatial motion of three-type particles, is constructed and the long-time behavior of the first …

Voices Of The "Served": Community Resident Perspectives On International Service-Learning In Peru., Adam C. Stieglitz

Electronic Theses and Dissertations

The purpose of this study is to capture and share community resident perspectives on international service-learning (ISL). Community resident voice is largely underrepresented in ISL scholarship and practice. Conversely, research on student outcomes is ubiquitous, which generally makes student benefit a predominant consideration in ISL program design. Consequently, scholars have criticized ISL programs for being inherently extractive and even an embodiment of modern-day colonialism. This study aims to address this critique by offering a robust representation of community residents’ perspectives for how to responsibly design ISL programs. This study used action research methodology to capture community resident voices who participate …

Targeting Pathological Changes In The Gut Protects The Cns From Neuropathology., Benjamin Tyler Charpentier

Electronic Theses and Dissertations

There is growing evidence that the health of the gut microbiome (GM) has an important role on multiple host organ systems. Ethanol-related neuropathologies and Alzheimer’s disease (AD) currently have no effective clinical treatment. Therefore, we developed a novel mouse model of fecal matter transplantation (FMT) utilizing the microbiome from alcoholic hepatitis (AH) patients, characterized by a loss of butyrogenic potential. Minimal neuroinflammation occurred in AH-FMT mice, but significant increases in the endoplasmic reticulum stress response (ERSR) across the majority of neurons in the prefrontal cortex and hippocampus were observed. In the cerebellum, Purkinje cells were specifically affected. Targeting the loss …

Search For A Dark Leptophilic Scalar Produced In Association With Taupair In Electron-Positron Annihilation At Center-Of-Mass Energies Near 10.58 Gev., Diptaparna Biswas

Electronic Theses and Dissertations

Dark matter is believed to be a form of matter which seemingly accounts for approximately 85% of the matter in the universe and about 27% of its total mass–energy density. It doesn't participate in electromagnetic interaction, i.e. doesn't interact with light. Consequently, we cannot see it using optical or radio telescope and hence the name dark matter. However, it participates in gravitational interaction, and we hypothesize its existence based on a variety of astrophysical observations, including gravitational effects, that cannot be explained by the accepted theories of gravity unless we account for more matter than can be perceived through electromagnetic …

Experimental Evaluation Of Heat Leak From Anti-Sweat Components Into Fresh Food And Freezer Compartments Of A Household Refrigerator., Delva Felissaint

Electronic Theses and Dissertations

Condensation beads forming on outside surfaces around a refrigerator is an undesirable byproduct driven by the cold temperature exhibited throughout the unit. To combat this issue, anti-sweat components usually in the form of electrical heaters or tubes carrying hot refrigerant, bring heat to the cold surfaces to ensure their temperatures do not drop below the dew point. Some heat produced by the anti-sweat components may transfer to the interior refrigerated compartment. The purpose of this study is to quantify how much heat from the anti-sweat components are entering the fresh food and freezer compartments and what impact that has on …

Classifying The 2022 Status Of Tsuga Canadensis (Eastern Hemlock) Along The Kentucky Portion Of The Pine Mountain Wildlands Corridor., Grace M. Embree

Electronic Theses and Dissertations

The invasion of the Hemlock Woolly Adelgid (HWA) (Adelges tsugae) has posed a continual threat in the United States to the Eastern Hemlock (Tsuga canadensis) trees since the 1950s. HWA feed on eastern hemlock needles, reducing the amount of healthy photosynthesizing vegetative area. The use of satellite imagery has been instrumental in identifying areas of eastern hemlock presence. Satellite platforms like Landsat and AVIRIS are commonly used for identification, classification, and mapping of eastern hemlock. Sentinel-2 imagery was released in 2015 for free access. It has a finer spatial grain of with the majority of the …

The On-Line Width Of Various Classes Of Posets., Israel R. Curbelo

Electronic Theses and Dissertations

An on-line chain partitioning algorithm receives a poset, one element at a time, and irrevocably assigns the element to one of the chains. Over 30 years ago, Szemer\'edi proved that any on-line algorithm could be forced to use $\binom{w+1}{2}$ chains to partition a poset of width $w$. The maximum number of chains that can be forced on any on-line algorithm remains unknown. In the survey paper by Bosek et al., variants of the problem were studied where the class is restricted to posets of bounded dimension or where the poset is presented via a realizer of size $d$. We prove …

Defining The Importance Of The Hnrnp I Interaction To The Sindbis Virus Subgenomic Viral Rna Using An Innovative Tethering Approach., Claire Westcott

Electronic Theses and Dissertations

Old World alphaviruses cause significant outbreaks of illness and debilitating multi-joint arthritis for prolonged periods. Currently, there are no FDA approved vaccines or antiviral therapies; and thus, there is a critical need to identify and characterize the molecular biology of alphaviruses. Alphaviruses rely on the host cell machinery to complete the viral lifecycle and are dependent on interactions with host RNA binding proteins. Accordingly, several host heterogenous nuclear ribonucleoprotein proteins (hnRNPs) have been found to bind to the Sindbis virus (SINV) RNAs. Disrupting the interaction sites in the viral RNAs of these RNA:Protein interactions results in decreased viral titers in …

Unraveling Dna And Identity: A Humanistic Perspective On Epistemologies And Ethics Of Genetic Ancestry Testing., Eve Carlisle Polley

Electronic Theses and Dissertations

The advent of DNA ancestry testing motivated a burst of human activities that constitute a scientific-technological-industrial-personal-social movement of immense scale, infused with epistemological and ethical questions of great and important variety. This movement has motivated many discourses in the social sciences, with study subjects ranging from the language usage of geneticists, to moral conundrums faced by test-takers, to potential ramifications in global structures of political power. At the same time, and especially in recent decades, the discourses of the comparative humanities have included with increasing frequency and urgency research and theorization about concepts and consequences of human social identities, alongside …

Disparate Sense Of Exclusion Between Young People Of Color Living Within Variable Social Infrastructures., James M. Joyce

Electronic Theses and Dissertations

I analyzed transcripts of listening sessions with youth/young adults of color in 2021-2022 for the purpose of addressing local racial inequity during COVID-19. I used inductive coding methods and found three themes on sense of exclusion to be most salient. These themes related to racial exclusion, exclusion of social infrastructures in the community, exclusion of young people of color by people working in schools and other public settings, and exclusion or disconnection of young people of color from opportunities for building community. I show how these themes vary across some dimensions of the local social infrastructure, and I discuss implications …

Novel Applications Of Hot Melt Extrusion For Developing Oral Solid Dosage Forms With Improved Solubility, Permeability, And Stability, Dinesh Nyavanandi

Electronic Theses and Dissertations

In today's world, with an increasing patient population, the demand for pharmaceutical medications is increasing enormously. However, pharmaceutical industries are continuously failing to meet market and patient populations' demands. Poor solubility of the drug substances is the major roadblock affecting the pharmaceutical industries in meeting the market demand and revenue. Around 70-80% of the new chemical entities within the developmental pipeline are claimed to be poorly soluble, affecting the oral bioavailability. Improving the solubility of drug substances by preserving their stability and physiological action is the primary prerequisite for developmental scientists. The underdeveloped manufacturing process with various limitations is another …

Effect Of Excipients/Solvents On The Barrier Integrity And Topical Drug Delivery Across Micropores, Abhishek Shettar

Electronic Theses and Dissertations

The topical and transdermal drug delivery system offers several advantages over conventional drug delivery; non-invasive, patient compliance, avoidance of first-pass metabolism, and sustaining therapeutic drug levels for several days. As a route for drug administration, skin presents a large surface area and can be used for both local and systemic drug delivery. However, the skin forms a formidable barrier protecting the human body from external environmental rigors and excessive water loss, maintaining equilibrium. The skin's barrier properties can be attributed to its unique macromolecular organization and morphology. However, the organized structure of the skin, since intended to prevent the entry …

The Effects Of Interactive Object Provisioning On Corticosterone, Stress-Related Behaviors, And Cognition In Juvenile And Adult Zebra Finches (Taeniopygia Guttata), Laura West

Electronic Theses and Dissertations

Identification and reduction of unnecessary stress in lab animals is important for welfare, and for scientific validity. Providing animals with infrastructure and objects that encourage exercise and species-typical manipulative behaviors can help reduce stress by meeting physical and psychological needs. The effects of such “environmental enrichment” (EE) have been heavily studied in both zoo animals—where it has been shown to reduce maladaptive behaviors—and in lab animals such as rodents, where it clearly reduces stress and anxiety, dampens reactions to stressful events, and increases cognition and the volume of related brain regions. While there is some evidence that EE is similarly …

Genomic Analysis And Metabolic Potential Of Myxobacteria, Hanan Albataineh

Electronic Theses and Dissertations

Myxobacteria are known for their large genomes that bestow them with a distinct lifestyle that is characterized by fruiting body formation, gliding motility, social interactions, predation, and secondary metabolite production. The powerful combination of whole-genome sequencing and bioinformatics-driven analysis of sequence data has delivered unexpected insights into myxobacterial microbial communities. We applied these two approaches to investigate the chemical space and biosynthetic potential of myxobacteria. Mining different myxobacterial genomes for biosynthetic gene clusters led us to the discovery of a cryptic acyl-homoserine lactone (AHL) synthase encoded in the genome of the myxobacterium Archangium gephyra. Bioinformatic analysis of this AHL synthase …

The Ties That Bind: Creole Networks And Reform In New Granada During The Age Of Revolution, 1780-1811, Philip John Baltuskonis

Electronic Theses and Dissertations

At the beginning of the nineteenth century, Spanish Americans were immersed in a practical Enlightenment that aimed to improve society during the Age of Revolution (1780-1850). In the city of Santafé de Bogotá, elites comprised of Spaniards and creoles (Spaniards born in the Americas) joined in scientific expeditions, higher education, creating newspapers, and proposing economic and educational reforms that all promised to bring prosperity to the Viceroyalty of New Granada (present-day Colombia, Venezuela, Ecuador, and Panama). Rather than a source of major confrontation, elites’ activities aligned with the Spanish Crown’s interest in turning its American kingdoms into centers of wealth. …

Low-Velocity Impact, Vibration And Shock Response Of Unstitched/Stitched E- And S- Glass/Vinyl Ester Composites, Shahriar Chowdhury

Electronic Theses and Dissertations

In this study, the effects of through-thickness stitching on the dynamic properties, low-velocity impact and shock responses of stitched and unstitched E- and S- Glass/Vinyl Ester composites have been investigated. The stiffness and loss factor (inherent damping) properties are obtained from impulse-frequency response experiments using the hammer excitation method. Drop weight impact testing machine was used to carry out low-velocity punch shear tests on the composite specimens following ASTM D3763 Standard, and shock tube apparatus was used to determine center point deflection under blast loading. The stitching increased the dynamic flexural modulus by 13% for E- and 8% for S- …

The Paleoecology Of High-Elevation Bison In The Greater Yellowstone Ecosystem And Implications For Modern Bison Conservation, Darian Bouvier

Electronic Theses and Dissertations

The national mammal of the United States, the American Bison (Bison bison) was once nearly extinct. Populations have recovered to the degree that thousands roam the Great Plains today. Due to their large numbers and body size, this species has an oversized impact on the ecological communities where it lives and is considered a keystone herbivore in modern North American grasslands. This study explores the detailed, seasonally resolved, paleoecology of seven bison from the Greater Yellowstone Ecosystem during the Late Holocene through stable isotope analyses and species niche modeling. Isotopic analyses of δ¹³C, δ¹⁵N, …

Adverse Childhood Experiences And Quality Of Life: A Mediating Role Of Physical Activity And Executive Function, Loni Parrish

Electronic Theses and Dissertations

ACEs have been associated with heightened risk for a range of chronic health problems, substance use, and cognition in adulthood (Center for Disease Control (CDC), 2019; Hinojosa et al., 2017). One potential protective factor is physical activity (McEwen, 2016; Wu et al., 2013). Physical activity is associated with sustaining overall health, improving mental health by reducing symptoms of depression and anxiety (Murri et al., 2019; Sharma et al., 2006; Tasci et al., 2019), and maintaining a healthy body weight and BMI (WHO, 2021). Therefore, this study examined whether barriers to physical activity, physical activity levels, and executive outcomes serve as …

Our Bodies, Our Stories: Mental And Physical Self Connection As A Protective Factor In Sexual And Gender Minorities, Emerson A. Todd

Electronic Theses and Dissertations

The mind and body connection has been actively examined in neuroscience and developmental psychology backgrounds. Sociological theorists posit ways that individuals develop a sense of self through mind, body, and the social world. While research on the connection between the mind and body in disabled and aging populations is common, less is known of the everyday impact of these connections for other minority populations. The present study uses mixed-methods research to explore the relationship between mind and body and the impacts on internal and social life. The quantitative aspect of the study found significant relationships between physical and mental health …

Evaluating Spatial-Temporal Patterns In Us Tornado Occurrence With Space Time Cube Analysis And Linear Kernel Density Estimation: 1950-2019, Darrell L. Wiser

Electronic Theses and Dissertations

This research estimated the spatial-temporal patterns of tornadoes in the continental United States from 1950-2019 using the National Weather Service Storm Prediction Center’s Severe Weather GIS (SVRGIS) database. This study employed Space-Time Cube Analysis and Linear Kernel Density (Kernel Density Linear Process, (KDLP)) rather than the standard Kernel Density Estimation (KDE) approach; to evaluate whether tornado hotspot locations and intensities shift over time.

The first phase of the study utilized KDLP to map changes in tornado hotspots and qualitatively assess decadal shifts in hotspot locations and intensities by occurrence and magnitude between decades using ArcGIS Pro and CrimeStat. Next an …

A Phenomenological Study Of Contributing Factors And Common Trends Related To Teacher Attrition In Western North Carolina, Preston Clarke

Electronic Theses and Dissertations

A phenomenological approach was used to identify contributing factors and common trends related teacher attrition by conducting interviews with teachers who decided to exit a North Carolina School District located in Western North Carolina. A qualitative approach allowed the researcher to gain valuable insight into the decision-making process of the 15 research participants. Data collection involved conducting one on one telephone interviews with participants by asking a series of open-ended questions that allowed for probing and follow-up questions. Analysis of researcher notes, evaluation of textual data produced by interviews, and observations allowed the qualitative investigator to identify emerging themes and …

Individual Characteristics Of Postsecondary Underachievement, Natasha Godkin

Electronic Theses and Dissertations

Underachievement in postsecondary education has been a growing concern for educators and researchers. Higher education institutions have implemented early alert systems to identify students performing below standards. This early warning system has major limitations that confine it to an identification only approach. The current study aimed to investigate the psychometric properties of the Student Attitude Assessment Survey-R (SAAS-R) in a postsecondary sample. First, a confirmatory factor analysis validated the SAAS-R in a postsecondary sample. Predictive validity was then investigated by identifying students as underachieving or achieving based on responses to the SAAS-R and via a regression-based discrepancy model (ability vs. …

Reduced Fuel Emissions Through Connected Vehicles And Truck Platooning, Paul D. Brummitt

Electronic Theses and Dissertations

Vehicle-to-infrastructure (V2I) and vehicle-to-vehicle (V2V) communication enable the sharing, in real time, of vehicular locations and speeds with other vehicles, traffic signals, and traffic control centers. This shared information can help traffic to better traverse intersections, road segments, and congested neighborhoods, thereby reducing travel times, increasing driver safety, generating data for traffic planning, and reducing vehicular pollution. This study, which focuses on vehicular pollution, used an analysis of data from NREL, BTS, and the EPA to determine that the widespread use of V2V-based truck platooning—the convoying of trucks in close proximity to one another so as to reduce air drag …

Look, But Don’T Touch: Impact Of Covid-19 On The Nicu Caregiver, Rebecca C. Otwell-Dove

Electronic Theses and Dissertations

Admission of an infant into the neonatal intensive care unit (NICU) is an experience that has been associated with a variety of both positive and negative health outcomes for parent and child. While both the setting name and the admitted population alludes to a sole focus on the affected infant, what often remains underrecognized is the impact on the ones who care for the newborn long before and longer after their NICU stay. Given the recency and unprecedented impact of the COVID-19 pandemic, very little research has examined the impact of COVID-19-specific stress on the experience of parents of infants …