Digital Commons Network^™

A Comparative Analysis Of Media Representation Of Us College Athletic Scandals In Traditional News Media And Mediated Communication Within The College, Colleen Starrs

Electronic Theses and Dissertations

This study critically analyzes organizational and mass media communication about the Penn State Child Sexual Abuse Scandal of 2011. The author examines the different ways in which Pennsylvania State University administrators and officials communicated the scandal internally to their community (campus community and surrounding city community) and compared it to how The New York Times communicated the scandal through their unique media platform to the world. The timeline for the data is from November 1, 2011, to July 31, 2012, as the scandal broke to the world, as well as, the months immediately after. The findings suggest that as the …

Materials Design With Polylactic Acid-Polyethylene Glycol Blends Using 3d Printing And For Medical Applications., Jeremiah R. Bauer

Electronic Theses and Dissertations

This thesis is an examination of two material systems derived from polylactic acid (PLA) and polyethylene glycol (PEG). PLA is a polymer commonly sourced from renewable sources such as starches and sugars. It is a relatively strong, biodegradable polymer, making it ideal for use in the body. Even though it has a relative high strength, PLA is also brittle leading to the use of plasticizers to increase flexibility. One such plasticizer is PEG, which is a material that can exist at room temperature as either a thin liquid, or a hard waxy solid depending on the molecular weight. The first …

Sum Frequency Generation Spectroscopy Of Simulated Protein Secondary Structures., Andrew J. Adams

Electronic Theses and Dissertations

Sum frequency generation (SFG) spectroscopy is an experimental technique for differentiating between various conformations and orientations of interfacial proteins. Combining a theoretical framework for SFG with molecular dynamics (MD) simulations provides a powerful tool for studying systems containing interfacial proteins with applications in cell transport, biofilms, and fermentation processes. Roeters’ method was used to calculate theoretical SFG responses for a variety of individual α-helix and β-sheet peptide secondary structures simulated using MD. Results show how the shape and locations of SFG amide I responses change with differences in hydrogen bonding patterns, peptide orientations, and SFG polarization combinations. The data presented …

Tumor-Targeted Double Emulsions For Ultrasound-Triggered Delivery Of Molecular Therapeutics., Connor S Centner

Electronic Theses and Dissertations

Cancer is the second leading cause of death in the United States, with 1.74 million new cancer cases diagnosed and 610,000 cancer deaths expected in 2018 alone. Current treatments often result in negative systemic effects and ineffective treatment of the tumor. Drug delivery vehicles have been developed for more effective local delivery methods, but many drug delivery vehicles lack spatial and temporal control. Targeted double emulsions are a new class of drug delivery vehicles which present a promising option for a high payload and controlled delivery. The purpose of our project was to develop and characterize an aptamer-chelated double emulsion …

The Empathy Mitigation: Empathy And Its Impact On Pain Perception And Altruistic Motivation, Amanda Daly

Electronic Theses and Dissertations

Empathy and its impact on pain perception has been studied narrowly with the focus being on participants receiving empathy during a pain procedure. This study reversed the focus and ran a standard cold pressor test (CPT) in the context of an empathy frame structured to elicit an empathic response for others from participants. It was hypothesized that the group receiving the empathic frame would have longer CPT times due to alterations in pain perception from empathy activation and these subjects’ self-reported state-trait empathy level would positively correlate with the increased times. 85 subjects participated with a control group of 43 …

Interplay Of Molecular And Nanoscale Behaviors In Biological Soft Matter, Nicholas Ciaffone

Electronic Theses and Dissertations

The complexity of biological soft matter at the sub-micrometer level is fundamentally correlated to the functionalities at the larger scale. Reflecting the level of heterogeneities in the properties of systems remains challenging when probing small scales, due to the mismatch between the area surveyed with the tools offering nanoscale resolution, such as atomic force microscopy (AFM), and the scale of natural variations inherent to biology. Hence, to understand the physiological and mechanical alterations that occur within a single cell relative to a cell population, a multiscale approach is necessary. In this work we show that it is possible to observe …

Tunable Effect Of Metal Ions On Polyelectrolyte Mechanics, Angie Diaz

Electronic Theses and Dissertations

Polyelectrolyte based hydrogel fibers can mimic extracellular matrix and have applications such as drug delivery and tissue scaffolding. Metal ions play a critical role in hydrogel fiber stability via electrostatic interactions, but knowledge of how they modulate mechanical properties of individual polyelectrolyte polymers is lacking. In this study, electrospun polyacrylic acid with chitosan is used as a model system to evaluate ferric ion effect on nanofiber mechanics. Using dark field microscopy imaging and persistence length analysis, we demonstrate that ferric ions modulate the bending stiffness of nanofibers. Young's modulus of individual nanofibers is estimated at values of a few kilopascals, …

Development Of Low Frequency Electron Paramagnetic Resonance Methods And Instrumentation For Biological Applications, Laura A. Buchanan

Electronic Theses and Dissertations

EPR is a powerful biophysical tool that can be used to measure tumor physiology. With the addition of magnetic field gradients, the spectral properties of paramagnetic species can be mapped. To facilitate EPR imaging, methods and instrumentation at frequencies between 250 MHz and 1 GHz were developed.

At low spin concentrations, the rapid scan background signal is often many times larger than the EPR signal of interest. To help remove the background contribution, a data acquisition procedure that takes advantage of a cross-loop resonator and bipolar power supplies was developed at 250 MHz. In this procedure, two scans are collected. …

Learning From Disorder And Noise In Physical Biology, Taylor Emil Firman

Electronic Theses and Dissertations

Stochasticity, disorder, and noise play crucial roles in the functioning of many biological systems over many different length scales. On the molecular scale, most proteins are envisioned as pristinely folded structures, but intrinsically disordered proteins (IDPs) have no such folded state and still serve distinct purposes within the cell. At the scale of gene regulation, realistic in vivo conditions produce stochastic fluctuations in gene expression that can lead to advantageous bet-hedging strategies, but can be difficult to characterize using a deterministic framework. Even at the organismal scale, germband extension (GBE) in Drosophila melanogaster embryos systematically elongates the epithelial tissue using …

Tragic Creation: Hope For The Future—Moltmann's Creative (Mis)Reading Of Hegel's Philosophy, John Michael Bechtold

Electronic Theses and Dissertations

Christian theology, in its many and varied forms, and to the detriment of both the church and the world, is often built upon a shaky epistemological foundation. In this dissertation, I describe this shaky foundation by the term 'insular universalism'. The oxymoronic nature of the term is both intentional and telling. A theology which strives for, or unwittingly arrives at, a position which is here being called 'insular universalism' achieves neither while rejecting or misunderstanding the complexity of both. When considered theologically, insular universalism could be simplistically described as the idea that "one cultural expression of the religion is exclusive …

Brønsted Acid Catalyzed Carbon-Carbon Bond Forming Reactions, Jiangyue Miao

Electronic Theses and Dissertations

The focus of this research project lies in the development of new methodology in the field of Brønsted acid catalysis enabling rapid synthesis of medicinally relevant compounds. It is foreseen that small molecule sulfonic acids evaluated in this research project will unveil new asymmetric carbon carbon bond forming reactions between substrates hitherto unexplored with Brønsted acid catalysis. It has been established that strong Brønsted acids, such as phosphoric acids, are capable of mediating highly selective transformations operating through unique mechanistic manifolds.

Specific focus for the sulfonic acid catalysts was geared towards asymmetric coupling reactions with synthetically useful precursors such as …

Diet Breadth Evolution And Diversification Of A Generalist Insect Herbivore, Mayra Cadorin Vidal

Electronic Theses and Dissertations

Insect herbivores are one of the most diverse groups of multicellular organisms, and the vast majority are specialists, which feed on only a few plant species. The factors that cause some herbivores to be specialists and others to be generalists are still unclear. It is known that the selective forces from natural enemies (top-down) and the host plants (bottom-up) influence an herbivore's diet breadth. In my meta-analysis evaluating the relative important of top-down and bottom-up forces on insect herbivore fitness, I found that herbivores usually have greater performance on better quality plants and in the absence or reduction of enemy …

Tau Aggregation, Conformational Selection, And Inhibition, Michael R. Holden

Electronic Theses and Dissertations

Tau fibrils are a pathological hallmark of over 20 neurodegenerative disorders, including Alzheimer's disease. There currently is no cure for these diseases and treatments are limited. Once Tau fibrils form in the brain, they propagate down neuronal networks, and this spreading is linked to disease progression. Studying the behavior and structure of Tau monomer and Tau aggregates therefore may give insight into methods by which the spread of Tau fibrils can be inhibited. The structures of the Tau fibrils from different diseases are thought to vary, partially giving rise to the different disease phenotypes. Tau natively binds to microtubules by …

Social Conflict On The Seas: Links Between Overfishing-Induced Marine Fish Stock Declines And Forced Labor Slavery, Jessica L. Sparks

Electronic Theses and Dissertations

Despite media attention detailing labor abuses in fisheries, social-ecological systems research has largely failed to consider whether fish stock declines could be contributing to increases in forced labor slavery. Empirical fisheries data suggests, though not a ubiquitous response to declining stocks, many vessels will fish longer, farther from shore, and deeper in waters to maintain yields. This effort intensification increases production costs, and Brashares et al. (2014), consistent with slavery theory, posited cheap and/or unpaid labor as an approach to offset increasing costs and continue harvesting fish species at a rate otherwise cost-prohibitive.

Using fuzzy cognitive mapping - a participatory, …

Psychotropic Medications And Children: Perceptions Of Mental Health Professionals, Elinor Jane Brereton

Electronic Theses and Dissertations

This project explores mental health professionals' perspectives on the prescription of psychotropic medications to children. It emphasizes the placement of biomedicine within its larger social, economic, and political context, and the influence these structures have on the way mental illness is conceptualized and treated in children. Eight semi-structured interviews were conducted in Denver, Colorado with psychiatrists, psychologists, social workers, and a pharmaceutical board member to capture multiple perspectives from different positionalities within the field. Participants discussed factors that they believe influence prescribing practices including: professional role changes, issues of access, limited evidence, cost, and institutional pressures to practice within a …

Away From The End Of Motherhood: Sites Of Haunting In The Social Imaginary In Lemonade And The Handmaid's Tale, Julia Michele Fleming

Electronic Theses and Dissertations

This thesis analyzes the television series adaptation of The Handmaid's Tale, specifically the episode "A Woman's Place," and Beyoncé's Lemonade: A Visual Album. I argue that these cultural texts leverage representations of women's lived experiences to scrutinize contemporary American anxieties about motherhood and reproductive justice. Lemonade, a celebration of Black womanhood, presents a counterpoint to The Handmaid's Tale's preoccupation with white motherhood in way that speculates on the utopian potentials of a woman-centered society.

Using bell hooks' film analysis, Avery Gordon's "haunting," and Luce Irigaray's "mimicry," I examine two interconnected themes: feminist aesthetics and generational haunting. …

Through The Yoruba Lens: A Postcolonial Discourse Of Female Circumcision, Jennifer Quichocho

Electronic Theses and Dissertations

Despite the Western media attention and the critique of female circumcision in sub-Saharan Africa, few studies consider the local populations' traditions, values, and ideologies. Through the Yoruba Lens: A Postcolonial Discourse of Female Circumcision investigates female circumcision practices from a philosophical, Yoruba traditionalist perspective. African philosophy and religion provides an ideological foundation and helps reveal the postcolonial and feminist theoretical framework that continues the academic debate. Framed by LeCompte and Schensul's notion that "ethnography emphasized discovery; it does not assume answers" (2010: 33), my research draws from literature reviews, quantitative data, and interviews. I will present and investigate three hypotheses …

Patient-Specific Quantification Of The Relationship Between The Left Atrium Pressure And The Ostial Diameter Of The Left Atrial Appendage, Sky Tianqi Gao

Electronic Theses and Dissertations

The left atrial appendage has been a historically understudied region of the heart until fairly recently with the new understanding of its role in the stroke pathway of patients with atrial fibrillation. The goal of this study is to take a look at the biomechanical behavior of the left atrium and left atrial appendage under normal physiological loading conditions using material properties taken from biaxial stretch tests. Several different options for material properties models were tested and biaxial stretch test data of cadaveric human tissue samples for the left atrium and appendage were fit to a Fung-type strain-energy function for …

Robust Distributed Stabilization Of Interconnected Multiagent Systems, Vahid Rezaei

Electronic Theses and Dissertations

Many large-scale systems can be modeled as groups of individual dynamics, e.g., multi-vehicle systems, as well as interconnected multiagent systems, power systems and biological networks as a few examples. Due to the high-dimension and complexity in configuration of these infrastructures, only a few internal variables of each agent might be measurable and the exact knowledge of the model might be unavailable for the control design purpose. The collective objectives may range from consensus to decoupling, stabilization, reference tracking, and global performance guarantees. Depending on the objectives, the designer may choose agent-level low-dimension or multiagent system-level high-dimension approaches to develop distributed …

Hidden In White(Ness)? Using Racial Logics To Interrogate The Instructional Arc Of A Crisis Intervention Team Training, Tara N. Meister

Electronic Theses and Dissertations

Some scholars argue that communities of color have been historically considered criminal by law enforcement (Fanon, 1963/2004; Gordon, 1996; Sharpley-Whiting, 1999), and Parenti (2003) and Kendi (2016) traced the history of modern policing to slave patrols. Wynter (2003) illuminated the racial logics undergirding our society - including policing - to the overlay of monotheism and its fixity with racial constructions of human as historically rooted in slavery and colonization. Thus, policing, too, carries a racialized dimension (Gamal, 2016) that creates or amplifies trauma and mental illness (Westcott, 2015). To address mental health, an undergirding feature of use of force and …

Effects Of Anthropogenic Noise On Mating Behavior And Fitness, Gabrielle A. Gurule-Small

Electronic Theses and Dissertations

When environments change rapidly, adaptive phenotypic plasticity can ameliorate negative effects of environmental change on survival and reproduction. Recent evidence, however, suggests that plastic responses to human induced environmental change are often maladaptive or insufficient to overcome novel selection pressures. Anthropogenic noise is a ubiquitous and expanding disturbance with demonstrated effects on fitness-related traits of animals like stress responses, foraging, vigilance, and pairing success. Elucidating the lifetime fitness effects of noise has been challenging because long-lived vertebrate systems are typically studied in this context. In both chapters described herein, I reared field crickets, Teleogryllus oceanicus, in masking traffic noise, …

Boitawl: Soil, Lost And Left, Bishnupriya Chowdhuri

Electronic Theses and Dissertations

Boitawl ("Boi"- lack, devoid of, "Tawl"- bottom/ ground/ foundation), the word in one of the Bengali dialects refers to one without a ground beneath her feet. The thesis, a hybrid collection of prose and verse including narratives and graphic vignettes, flash, fabulist and short stories, prose poems and free verse imagines the inside worlds of such un-settled existences. In the process, the pieces connect migration, memory, childhood and lost towns with fractured humans caught in between - to reveal what lies under pillars of desires, the shapes of unsaid longings and recurrent images in their dreams.

Using Dialogue To Interrupt Legacies Of Exclusion, Incite Hope, Invite Change, And Increase Levels Of Awareness At Predominantly White Institutions With Diversity, Equity, And Inclusion Rhetoric, Amanda Meise

Electronic Theses and Dissertations

This dissertation questions how a predominantly white institution (PWI) could infuse dialogue to aid the implementation of diversity, equity, and inclusion (DEI) initiatives and invite institutional change. There has been an increased spotlight on racial tensions permeating predominantly white campuses with DEI initiatives; higher education scholars have identified several factors that prevent institutions from fostering inclusive spaces. This research addresses three specific hurdles for PWIs implementing DEI initiatives: (1) social amnesia characterized by romanticized versions of history; (2) a discontinuity between professed values and the goals of DEI initiatives with policy, structure, and experience; and (3) low awareness of privilege …

Rnn-Based Generation Of Polyphonic Music And Jazz Improvisation, Andrew Hannum

Electronic Theses and Dissertations

This paper presents techniques developed for algorithmic composition of both polyphonic music, and of simulated jazz improvisation, using multiple novel data sources and the character-based recurrent neural network architecture char-rnn. In addition, techniques and tooling are presented aimed at using the results of the algorithmic composition to create exercises for musical pedagogy.

Symmetry Identified In 2-Dimensional Artwork Compositions Using Visuospatial Ability, Theresa Ferg

Electronic Theses and Dissertations

At the John Langdon Down Foundation A.C. in the La Escuela Mexicana de Arte Down school in Mexico City D.F., Mexico, art students with Trisomy 21 display the use of a mathematical construct in the painting compositions of their artworks. The mathematical construct is a type of symmetry and it carries a positive affect. This is important because there have been no studies that have investigated the use of the symmetry in the artwork compositions of persons with Down syndrome. The geometric construction of the artwork compositions follows the artistic principle of the Rule of Three and the division of …

Origins Of Animal Cell Adhesion, Jennyfer Mora Mitchell

Electronic Theses and Dissertations

A fundamental requirement for multicellularity is cell adhesion. This includes mechanisms by which cells adhere to each other and to their secreted extracellular matrix (ECM). Two well characterized adhesion complexes in animals include: 1) adherens junctions (AJs), which are involved in cell-cell adhesion and composed of cadherin receptors, p120-, α- and β-catenin, and 2) focal adhesions (FAs), which are involved in cell-ECM adhesion and include proteins such as integrins, vinculin, paxillin, talin and focal adhesion kinase (FAK). The molecular components of AJs are widely conserved in animals, and largely absent in non-animals. In contrast, the molecular components of FAs have …

Improving The Detection Limit Of Tau Aggregates For Use With Biological Samples, Emily Rickman Hager

Electronic Theses and Dissertations

The protein Tau is found in neurofibrillary tangles in Alzheimer's disease and over 20 other neurodegenerative diseases. An assay has been developed to detect minute amounts of fibrils from human brain tissue. This assay subjects brain tissue extract and recombinant Tau to several rounds of sonication and incubation. Incubation allows recombinant Tau to add itself to the ends of the existing fibrils in brain tissue extract. Sonication breaks the existing fibrils in the brain tissue extract offering more ends for Tau to add onto. Cycles of sonication and incubation have been shown to allow for amplification of Tau fibrils from …

Relationship Between Tdp-43 Toxicity And Aggregation In Saccharomyces Cerevisiae, Martin Anthony Aguilar

Electronic Theses and Dissertations

Protein aggregation and inclusion body formation are hallmarks of neurodegenerative diseases such as Alzheimer's, Parkinson's, Huntington's, and amyotrophic lateral sclerosis (ALS). These neurodegenerative diseases share a common pathology in that all include accumulation of insoluble protein aggregates in the brain. TAR-DNA-binding protein (TDP-43) is the major component found in the pathological inclusions of two of these diseases, ALS and frontotemporal lobar degeneration with ubiquitin-positive inclusions (FTLD-U). This thesis focuses upon the biophysical basis for TDP-43 aggregation in S. cerevisiae. Current in vitro evidence indicates that TDP-43 is a natively dimeric protein and that binding to RNA inhibits aggregation. Corresponding …

Photochemistry Of Polycyclic Aromatic Hydrocarbons With Environmentally Relevant Metals, John P. Haynes

Electronic Theses and Dissertations

The details of the work presented in this dissertation focus on simulated reactions in the atmosphere and water systems between persistent aromatic compounds and varying species of metals with an emphasis on iron. Bulk water suspensions of a standard soil and polycyclic aromatic hydrocarbons (PAH) were analyzed for soluble iron by inductively-coupled plasma mass spectroscopy (ICP-MS) following a 16 hour reaction period using simulated sunlight. Significant increases in soluble iron were only seen with 2-3 ring linear PAH and carboxylic acids.

A two-stage study was conducted to investigate the potential for and possible mechanisms involving the photo-oxidation of PAH into …

Superresolved Three-Dimensional Analysis Of The Spatial Arrangement Of The Human Immunodeficiency Virus Type-1 (Hiv-1) Envelope Glycoprotein At Sites Of Viral Assembly, Carmen Anne Buttler

Electronic Theses and Dissertations

Human Immunodeficiency Virus type 1 (HIV-1) replicates by forcing infected host cells to produce new virus particles, which assemble form protein components on the inner leaflet of the host cell's plasma membrane. This involves incorporation of the essential viral envelope glycoprotein (Env) into a structural lattice of viral Gag proteins. The mechanism of Env recruitment and incorporation is not well understood. To better define this process, we seek to describe the timing of Env-Gag encounters during particle assembly by measuring angular positions of Env proteins about the surfaces of budding particles. Using three-dimensional superresolution microscopy, we show that Env distributions …