Digital Commons Network^™

Localization Of Sip470, A Plant Lipid Transfer Protein In Nicotiana Tabacum, Shantaya Andrews

Electronic Theses and Dissertations

SABP2-interacting protein 470 (SIP470), a non-specific lipid transfer protein (nsLTP), was discovered in a yeast two-hybrid screening using SABP2 as bait and tobacco leaf proteins as prey. SABP2 is an important enzyme in systemic acquired resistance that converts salicylic acid to methyl salicylate. Localization studies are an important aspect to understanding the biological function of proteins. nsLTPs are generally considered apoplastic proteins and has been localized intracellularly and extracellularly. Transient expression shows highest expression of SIP470-eGFP at 2 days post infiltration into Nicotiana benthamiana. Confocal microscopy showed localization near the periphery of the cell. Subcellular localization using differential centrifugation showed …

The Study Of Alcoholic Liver Diseases, Seun Emmanuel Owoseni

Electronic Theses and Dissertations

Excessive alcohol consumption is the primary contributing factor in the development of alcoholic liver diseases (ALD). Nicotine contained in tobacco is a major addictive alkaloid, which enhances the effects of ALDs. The major enzyme involved in nicotine metabolism is cytochrome P450 2A5 (CYP2A5) which is produced in the liver. Alcohol can stimulate the CYP2A5 enzyme. We utilized cyp2a5-/- knockout mice in this research to examine the effects of CYP2A5.

The cyp2a5-/- mice and wild-type (WT) mice were fed liquid ethanol diet with or without nicotine to induce ALD. Nicotine enhancing effects on ALD were observed in WT mice but not …

The Role Of Cyp2a5 In Cadmium-Induced Liver Injury, Julia Salamat

Electronic Theses and Dissertations

Cadmium is present in food and groundwater. Tobacco smoking and occupational exposure are also major sources for cadmium. Cadmium is primarily accumulated in liver, a major organ metabolizing exogenous chemicals. Chemical metabolism may cause detoxification, but it can also cause bio-activation resulting in liver damage. Cytochrome P450s (CYP) are major liver metabolism enzymes, and cadmium chloride (CdCl2) can induce CYP2A5 in mice. We examined the effect of CYP2A5 on CdCl2-induced liver injury using CYP2A5-knockout (cyp2a5-/-) mice. The cyp2a5-/- mice and their control WT mice were injected CdCl2 intraperitoneally at 5 mg/kg body weight, respectively, to induce liver injury. …

Imidacloprid Persistence, Mobility, And Effect On Ecosystem Function, Joanna Hardin

Electronic Theses and Dissertations

Imidacloprid is a neonicotinoid pesticide used to protect against biting and sucking insects. Land managers rely on its systemic properties, however long-term studies investigating imidacloprid effects on ecosystem function are limited. This study investigated imidacloprid applications to Tsuga caroliniana and Tsuga canadensis over time and compared concentrations to measures of ecosystem function including soil respiration, microbial function, and invertebrate density. Results indicate that imidacloprid is persistent (p0.5). Microbial function and invertebrate density were not significantly different between control and treatment locations nor did imidacloprid concentrations correlate with ecosystem functional indicator activity (p>0.05). It is evident that imidacloprid does not …

Epidermal Growth Factor Receptor (Egfr) Inhibition By Polychlorinated Biphenyls Contributes To Non-Alcoholic Fatty Liver Disease (Nafld)., Josiah Hardesty

Electronic Theses and Dissertations

This dissertation describes how poly-chlorinated biphenyls (PCBs) exacerbate the pathogenesis of non-alcoholic fatty liver disease (NAFLD). While PCBs were banned in 1979, they still persist in contaminated biota, including food, and are detected in human plasma and adipose. The body burden of PCBs is associated with elevation of liver enzymes and necrosis markers in humans, characteristic of NAFLD. PCB exposure in high-fat diet fed mice leads to steatohepatitis that recapitulate the findings seen in exposed humans. The global estimate of people diagnosed with NAFLD is up to 1 in 4 people, unrelated to dietary or genetic factors. The hepatic mechanisms …

Characterization Of Sip68 For Its Role In Plant Stress Signaling, Saroj Chandra Lohani

Electronic Theses and Dissertations

Glucosyltransferases catalyze the transfer of glucose molecules from an active donor to acceptor molecules and are involved in many plant processes. SIP68, a tobacco glucosyltransferase protein, is a SABP2-interacting protein. It was identified in a yeast two-hybrid screen using SABP2 as bait and tobacco proteins as prey. SABP2, converts methyl salicylate to salicylic acid (SA) as a part of the signal transduction pathways in SA-mediated defense signaling. Subcellular localization is a crucial aspect of protein functional analysis to assess its biological function. The recombinant SIP68 tagged with eGFP was expressed transiently in Nicotiana benthamiana and observed under confocal microscopy. Fluorescent …

Computational Quantum Chemistry Studies Of The Interactions Of Amino Acids Side Chains With The Guanine Radical Cation., Edward Acheampong

Electronic Theses and Dissertations

Guanine is generally accepted as the most easily oxidized DNA base when cells are subjected to ionizing radiation, photoionization or photosensitization. At pH 7, the midpoint reduction potential is on the order of 0.2 – 0.3 V higher than those of the radicals of e.g. tyrosine, tryptophan cysteine and histidine, so that the radical “repair” (or at least, a thermodynamically favorable reaction) involving these amino acids is feasible. Computational quantum studies have been done on tyrosine, tryptophan, cysteine and histidine side chains as they appear in histones. Density functional theory was employed using B3LYP/6-31G+ (d, p) basis set to study …

“I’Ve Always Identified With The Women:” How Appalachian Women Ballad Singers’ Repertoire Choices Reflect Their Gendered Concerns, Sara Lynch-Thomason

Electronic Theses and Dissertations

This thesis explores how contemporary Appalachian women’s gendered experiences influence their choices of ballad repertoire. This inquiry is pursued through a feminist analysis of interviews with six women ballad singers from Madison County, North Carolina. In evaluating the women’s choices of ballads and their commentary on the songs, this thesis draws upon narratological theories as well as concepts from Appalachian traditional music studies.

This study finds that women’s repertoire preferences reveal contemporary female concerns for physical safety and political agency. The singers also extract hidden transcripts from ballad texts and use ballads to educate audiences about women’s historic oppression. However, …

Undergraduate Student Perceptions Of Ap And Dual Enrollment In Relation To College Readiness Skills, Ashleigh E. Norris-Shu

Electronic Theses and Dissertations

The purpose of this quantitative study was to determine if undergraduate students perceived that their college readiness was impacted by participation in Advanced Placement courses, dual-enrollment courses, or both. Perceptual data were gathered from freshmen and sophomores enrolled at East Tennessee State University using an online survey. The number of participants in this study was 265. Perceptions of the individual program components of both Advanced Placement and dual-enrollment courses were also assessed in relation to college readiness. The results of the study indicated that undergraduate students perceive instructor quality and course rigor of both AP and dual-enrollment as beneficial to …

Self-Compassion And Physical Health-Related Quality Of Life In Cancer: Mediating Effects Of Control Beliefs And Treatment Adherence, Morgan Treaster

Electronic Theses and Dissertations

Among the 14 million persons living in the United States with current or remitted cancer, poor physical health-related quality of life (HRQL) is a significant concern. However, self-compassion (i.e., common humanity, mindfulness, self-kindness) may be a protective factor, either directly or indirectly, by allowing for a sense of empowerment and control over illness, and in turn, facilitating engagement in treatment and positive perceptions of health. Serial mediation analyses among persons living with current (n = 67) or remitted (n = 168) cancer lend support for a positive, direct association between self-compassion and physical HRQL, as well as indirect effects via …

Gaming Behaviors In Day-To-Day Life: Exploring A Problematic Gaming Behavior Scale, Christin Collie

Electronic Theses and Dissertations

Growing concern for the problematic use of video games has prompted new research in a now growing field of literature. Internet Gaming Disorder (IGD) is a proposed behavioral addiction in the Diagnostic and Statistical Manual of Mental Disorders, fifth edition (DSM-5). To examine the proposed criteria for IGD, a self-report problematic gaming behavior scale (PGBS) was developed for adults residing in the United States. One hundred eighty-nine participants completed an online questionnaire comprised of demographic items, general gaming information, the PGBS, and the Internet Gaming Disorder-20 Test (IGD-20 Test). Statistical analyses conducted at the conclusion of the study were consistent …

Worry In The Pews: Exploring Levels And Causes Of Worry Among Church-Goers, Jennifer Lee

Electronic Theses and Dissertations

A large body of research indicates that worry, anxiety, and depression are present in the modern American psyche at increasingly high levels. Everyday worry can lead to further mental health issues, interpersonal problems, and reduced physical wellbeing and should be reduced when possible. Previous research has examined the benefits of church attendance with varied results; however, understanding the relationship between church attendance and everyday worry could be useful in reducing the effects of worry on this population.

The purpose of this quantitative study was to measure self-reported levels and causes of everyday worry among church-goers from three congregations and to …

Sip-428, A Sir2 Deacetylase Enzyme And Its Role In Biotic Stress Signaling Pathway, Bal Krishna Chand Thakuri

Electronic Theses and Dissertations

SABP2 (Salicylic Acid Binding Protein 2) plays a vital role in the salicylic acid signaling pathway of plants both regarding basal resistance and systemic acquired resistance against pathogen infection. SIP-428 (SABP2 Interacting Protein-428) is a Silent information regulator 2 (SIR2) like deacetylase enzyme that physically interacts with SABP2 in a yeast two-hybrid interaction and confirmed independently by a GST pull-down assay. We demonstrated that SIP- 428 is an NAD+ dependent SIR2 deacetylase enzyme. Transgenic tobacco plants silenced in SIP- 428 expression via RNAi showed enhanced basal resistance to microbial pathogens. Moreover, these SIP-428-silenced lines also exhibited a robust induction of …

Relative Permittivity As A Function Of Co-Solvent And Impedance Spectroscopy For Quantifying Anions In Solution, Yahya Sami Alseiha

Electronic Theses and Dissertations

This work measured relative permittivity from 15.0 to 55.0 °C of aqueous solutions containing acetone or tetrahydrofuran using a BI 870 dielectric meter. A cubic polynomial fit resulted in coefficients for calculating permittivity as a function of temperature and mole fraction. Literature values for other co-solvent system resulted in similar polynomial coefficients. Using BI 870, permittivity measurements of ionic solutions were not possible because of high conductivity. A simple method using impedance spectroscopy measured these solutions. Impedance decreases with increasing temperature and decreases with increasing ionic strength. Due to the temperature dependence of impedance, all measurements were at 25.0 °C. …

Dreadd Targeting Projections From The Nucleus Accumbens To The Ventral Pallidum With Nicotine Self-Administration, Amanda Smith

Electronic Theses and Dissertations

Projections from the Nucleus Accumbens (NAc) to the Ventral Pallidum (VP) play a critical role in motivation and reward. Rewards and reward-associated cues are thought to alter this pathway by suppressing GABA release to the VP, however, the role of the NAc to VP pathway has never been investigated with regard to nicotine self-administration. We hypothesized that increasing GABAergic signaling from the NAc to the VP would decrease the reinforcing effects of nicotine. To increase GABA release, CRE dependent DREADD was expressed in the NAc of male rats. Administration of low dose clozapine activated the DREADD receptor and showed a …

Characterization Of Acyltransferases And Wrinkled Orthologs Involved In Tag Biosynthesis In Avocado, Md Mahbubur Rahman

Electronic Theses and Dissertations

Triacylglycerols (TAG) or storage oils in plants are utilized by humans for nutrition, production of biomaterials and fuels. Since nonseed tissues comprise the bulk biomass, it is pertinent to understand how to improve their TAG content. Typically, the final step in TAG biosynthesis is catalyzed by diacylglycerol (DAG) acyltransferases (DGAT) and/or phospholipid: diacylglycerol acyltransferases (PDAT), which also determine the content and composition of TAG. Besides enzymatic regulation of TAG synthesis, transcription factors such as WRINKLED1 (WRI1) play a critical role during fatty acid synthesis. In this study, mesocarp of Persea americana, with > 60% TAG by dry weight and oleic …

The Role Of Non-Neuronal Acetylcholine In Urogenital Chlamydial Infection, Jessica R. Lockhart

Electronic Theses and Dissertations

Chlamydia trachomatiscauses a bacterial sexually transmitted infection, Chlamydia, that is often chronic and casues reproductive complications in women. We hypothesized that Chlamydia infection increases local acetylcholine (ACh) production, which regulates the host’s inflammatory response to the infection. Female mice infected with C. muridarumwere sacrificed at days 3, 9, 15, and 21 post-infection, genital tract tissues harvested, and immunohistochemistry performed to enumerate ACh-producing cells. Infection increased the number of ACh-producing cells in cervical tissue at days 3,15, and 21 post-infection (pi), uterine tissue at day 3 and 9 pi, and ovarian tissue day 3, 15, and 21 pi. These …

Temperature And Polyunsaturated Fatty Acid’S Effect On Daphnia Magna Reproduction, Mark Albright

Electronic Theses and Dissertations

Organisms adapt to their environments by adjusting their biochemistry and physiology; such adaptation is limited by resource availability and physiological constraints. The freshwater crustacean Daphnia magna inhabits a wide range of environments and must survive and reproduce within a range of temperatures. One limit to low-temperature adaptation is thought to be the availability of unsaturated fatty acids necessary to maintain proper fluidity of cellular membranes. D. magna maintained at 10 ºC on a diet poor in unsaturated fatty acids have been observed to produce clutches that fail to develop. However, this has not been observed on a diet rich in …

The Ecology Of Fecal Indicators, Dennis A. Gilfillan

Electronic Theses and Dissertations

Animal and human wastes introduce pathogens into rivers and streams, creating human health and economic burdens. While direct monitoring for pathogens is possible, it is impractical due to the sporadic distribution of pathogens, cost to identify, and health risks to laboratory workers. To overcome these issues, fecal indicator organisms are used to estimate the presence of pathogens. Although fecal indicators generally protect public health, they fall short in their utility because of difficulties in public health risk characterization, inconsistent correlations with pathogens, weak source identification, and their potential to persist in environments with no point sources of fecal pollution. This …

Extraction And Purification Of Biologically Active Metabolites From Rhodococcus Sp. Mtm3w5.2, Mohrah Alenazi

Electronic Theses and Dissertations

Rhodococcushas been recognized as a potential antibiotic producer. Recently, a strain of Rhodococcussp. MTM3W5.2 was isolated from a soil sample collected in Morristown, Tennessee and was found to produce an inhibitory compound which is active against other related species. The purpose of this research is to extract, purify and analyze the active metabolite. The compound was extracted from RM broth cultures and purified by preliminary fractionation of crude extract through a Sephadex LH-20 column. Further purification was completed using semi-preparative reversed phase column chromatography. Final purification was obtained using multiple rounds of an analytical C18 HPLC column. Based …

Performance Assessment Of The Extended Gower Coefficient On Mixed Data With Varying Types Of Functional Data., Obed Koomson

Electronic Theses and Dissertations

Clustering is a widely used technique in data mining applications to source, manage, analyze and extract vital information from large amounts of data. Most clustering procedures are limited in their performance when it comes to data with mixed attributes. In recent times, mixed data have evolved to include directional and functional data. In this study, we will give an introduction to clustering with an eye towards the application of the extended Gower coefficient by Hendrickson (2014). We will conduct a simulation study to assess the performance of this coefficient on mixed data whose functional component has strictly-decreasing signal curves and …

Public Perceptions On Domestic Sex Trafficking And Domestic Sex Trafficking Victims: A Quantitative Analysis, Faith Browder

Electronic Theses and Dissertations

Sex trafficking is a grossly misconstrued and increasing issue within the United States. The purpose of this study was to extend current knowledge regarding the public’s education on domestic sex trafficking and perceptions of domestic sex trafficking victims. The public’s awareness of domestic sex trafficking and perceptions of domestic sex trafficking victims were examined through the utilization of a 31 closed-ended question survey. The survey included questions about domestic sex trafficking and prostitution myths, domestic sex trafficking victim characteristics, domestic sex trafficking legislation, law enforcement’s involvement in domestic sex trafficking cases, and demographics. The sample consisted of 195 Criminal Justice …

Self-Regulation Abilities Of Low-Income Mothers In Community Based, Expanded Food And Nutrition Education Program, Alyssa Abreu

Electronic Theses and Dissertations

Diet is strongly associated with many risk factors for chronic disease. Educational programs such as the Expanded Food and Nutrition Education Program (EFNEP) are designed to improve dietary behaviors among low-income populations. Although EFNEP has seen improvements, they are not yet up to recommendations. Previous studies have been successful in eliciting behavior change when improving self-regulation in conjunction with dietary education. Thus, the purpose of this study was to examine self-regulation in EFNEP participants and its relationship to participants change in dietary behavior. All participants were currently enrolled in an EFNEP program and were asked to complete an additional self-regulation …

The Impact Of Mother And Father Stress On Child Externalizing Behaviors, Allison Mccobin

Electronic Theses and Dissertations

The impact of parental stress on child behavioral problems has often been examined through research. A large majority of research indicates a strong correlation between parenting stress and an increase in child behavioral difficulties. However, most studies have focused only on the impact that one parent’s stress has on the child’s behavior, rather than both or comparing the two. Using two separate cross-lagged structural equation models, data from the Fragile Families Child and Wellbeing Study (N = 1010) were analyzed to examine the differences between mother and father parenting stress on child externalizing behavior problems over time (ages three and …

Posttraumatic Growth: The Lived Experience Of Recurrent And Multiple Trauma Survivors Of Kenya’S Skirmishes, 2007 Post-Election Violence, And 2009 National Level Oil Tanker Fire Explosion, Jane C. Lang'at

Electronic Theses and Dissertations

Current research has associated the benefits of posttraumatic growth (PTG) with high levels of stress and trauma that challenge the victim’s assumptive world. The literature reveals that extensive research on PTG has been done on the American, European, and Asian continents but not on the African continent. Yet, how trauma survivors make meaning out of their lived experiences may not be the same globally. This universal phenomenon may also have varied interpretations based on the frequency, intensity, and scope of the traumata, along with the survivors’ socio-economic status (SES) and other benefits and risk factors. In addition, anticipation for high-risk …

Examining The Legacy Of Transgenerational Trauma And Its Effects On Contemporary African American Adults In Parenting And Caregiver Roles To African American Adolescents, Amy E. Alexander

Electronic Theses and Dissertations

The system of institutional enslavement in North America has produced myriad effects on the contemporary African American community via the transmission of individual, familial, and collective trauma across generations (Carter, 2015; DeGruy, 2005; Weingarten, 2004). This research explored the roles of parent and caregiver within this cultural group in an effort to determine how past traumas have influenced the lived experiences and world view of African Americans in these roles.

While research on certain historically marginalized cultural and religious groups is plentiful, there is a clear lacuna of scholarly investigation into the African American experience, particularly as it relates to …

Re-Queering The Trans Binary: Gender Nonconforming Individuals’ Experiences In Counseling And Therapeutic Settings, Michael Stephens

Electronic Theses and Dissertations

This study sought to unearth the narratives of gender nonconforming (GNC) individuals’ experiences of mental health services. The term gender nonconforming refers to individuals who do not identify with a strictly male or female concept of gender identity. There is an insubstantial research that has been conducted into the provision of effective mental health services for gender nonconforming individuals. Most of the studies in the literature review used the term transgender to highlight gender minority experience of counseling.

This study used gender nonconforming to separate from this terminology confusion. Individuals who identify with the identity label of transgender can be …

Self-Assembling Bioaffinity Hydrogels For Localized Antibody Delivery, Wen Liu

Electronic Theses and Dissertations

The scope of illnesses that are being treated using monoclonal antibodies (mAbs) has been expanding from cancers to autoimmune diseases due to their high specificity to their molecular target. Because many of the new mAbs are aimed to modulate the immune system, an emerging concern is that patients with chronic illnesses are subjected to immune-related Adverse Events (irAEs). Loss of immunological tolerance to self-antigens or increased susceptibility to infections have been documented in some mAb treatments. Localized sustained release of mAb at disease sites might mitigate the risk of such toxicities. Antibody drugs are formulated typically for parenteral delivery because …

Psychological And Family Variables Associated With The Treatment Of Pediatric Idiopathic Pain, Rachael Hoffman

Electronic Theses and Dissertations

The present study examined variability in treatment outcomes of children with idiopathic chronic pain. Specifically, the biopsychosocial model was used as a framework to consider the way that symptoms of depression and anxiety as well as parent response to pain contributed to the change in a child’s confidence in participating in daily activities following treatment in an inpatient chronic pain treatment program.

Economics Of Managing Loblolly Pine Plantations For Increased Wildlife Biodiversity, Melissa Griffith

Electronic Theses and Dissertations

The goal of this thesis was to identify the differences between the costs incurred and revenues gained from managing loblolly pine (Pinus taeda) plantations in East Texas for strictly timber production or a combination of timber production and wildlife biodiversity. Management regimes evaluated had varying planting densities, thinning frequency and intensities, prescribed burn intervals, and site indices. Economic analyses used to evaluate management regimes over a range of nonindustrial private landowner (NIPF) alternative rates of return (ARRs) and site indexes were net present worth, land expectation value, and equal annual annuity. These economic analyses demonstrated that altering management …