Digital Commons Network^™

Does Thermotolerance In Daphnia Depend On The Mitochondrial Function?, Rajib Hasan

Electronic Theses and Dissertations

Thermotolerance limit in aquatic organism is set by the ability to sustain aerobic scope to sudden temperature shifts. This study tested the genetic and plastic differences in thermotolerance of Daphnia that can be explained by the differences in the ability to retain mitochondrial integrity at high temperatures. Five genotypes with different biogeographic origins were acclimated to 18^ᵒC and 25^ᵒC. We developed a rhodamine 123 in-vivo assay to measure mitochondrial membrane potential and observed higher fluorescent in heat damaged tissues as the disruption of the mitochondrial membrane potential. Significant effects on temperature tolerance were observed with CCCP …

Purification Of Biomass Hydrolyzates For The Promotion Of Xylose Diester Formation, Laura Fernandez

Electronic Theses and Dissertations

The application of a novel xylose isolation procedure using phenyl boronic acid esterification has been limited to the hydrolyzates of low extractive biomasses like hardwoods and agricultural resides. Hydrolyzates with lower sugar and higher extractive concentrations, like softwoods, have not yielded xylose, or even xylose diester (XDE), the first intermediate product in the new process, under the same conditions. This research applied evaporation concentration, adsorbent separation and membrane filtration to these lower performing biomass hydrolyzates to maximize the yield of the XDE formation step of the new xylose extraction process. Lab-scale tests of these techniques were applied to pine hydrolyzate …

Fluid Flow Analysis Of Diluted Evaporating American Whiskey Droplets., Martin J. Brown Vi

Electronic Theses and Dissertations

The recent discovery of the unique structures, whiskey webs, formed when the evaporation of diluted American whiskey, has raised many questions as to the nature of the structures. Their formation process follows as such: (1) dilution of the whiskey to form nanoaggregates, (2) formation of a monolayer at the air-liquid interface, (3) chaotic ethanol evaporation caused monolayer collapse (via dynamic pressure), (4) bulk fluid evaporation caused monolayer collapse (via reduction of surface area), where the web-like structures reside on the surface, and finally, (5) web-like structures deposit on the substrate. The webs imaged via SEM had a striking resemblance to …

Synthesis, Characterization And Biological Evaluation Of Novel (S,E)-11-[2-(Arylmethylene) Hydrazono] Pyrrolo [2,1-C] [1,4] Benzodiazepine Derivatives, David Mingle

Electronic Theses and Dissertations

Pyrrolo [2,1-c] [1,4] benzodiazepine (PBD) is a class of natural products obtained from various actinomycetes which have both anti-tumor and antibiotic activities and can bind to specific sequences of DNA. PBD-dilactam was initially produced using isatoic anhydride and (L)-proline which was then converted to the PBD-thiolactam using Lawesson's reagent. Reaction of thiolactam with hydrazine in ethanol afforded PBD-11-hydrazinyl. Condensation of 11-hydrazinyl PBD with aldehydes possessing various substitutions was performed to obtain (S,E)-11-[2-(arylmethylene) hydrazono] pyrrolo [2,1-c] [1,4] benzodiazepine derivatives. ¹HNMR, ¹³C-NMR, DEPT, IR, GC-MS and X-ray crystallography were used for the characterization. Inhibition activity of the products …

The Salivary Alpha-Amylase Response To Resistance Training, Asher Flynn

Electronic Theses and Dissertations

The purposes of this dissertation were to investigate the response of salivary alpha-amylase to a single resistance training session and to a week-long resistance training over-reaching protocol. The major findings of this dissertation are as follows:

Study 1 – A single resistance training session consisting of 5 sets of 10 repetitions of squat and bench press at 95 percent of repetition maximum creates a statistically significant increase in salivary alpha-amylase concentrations.

Study 2 – Two resistance training sessions consisting of 5 sets of 10 repetitions of squat and bench press at 95 percent of repetition maximum within 5 days does …

Assessing Heat-Related Mortality And Morbidity Risks In Rural Populations And Sub-Populations, Emmanuel A. Odame

Electronic Theses and Dissertations

Heat stress is an environmental and occupational hazard exacerbated by climate change. Rural populations and sub-populations continue to experience disproportionate risks of heat-related impacts due to their low adaptive capacities in terms of infrastructure, information and other resources which are critical in dealing with heat. The study goals were to determine heat-related mortality risks in rural populations globally, explore the contribution of the outdoor work environment and other factors in association with occupational heat-related illnesses (HRI), and assess the risk of heat stress among crop workers using the Wet Bulb Globe Temperature (WBGT). Published peer-reviewed scientific literature on heat-related mortality …

Comparative Spending Of Medicaid Dollars On Child Participants Of Kentucky’S Sobriety Treatment And Recovery Teams Program Versus A Matched Comparison Group., Matthew Thomas Walton

Electronic Theses and Dissertations

Child protective services agencies have long observed the complicating role that parental substance use and addiction plays in cases of child maltreatment. Families who struggle with these problems present unique challenges for child welfare professionals. These families are typically more difficult to engage, more likely to have children removed from the home, and have poorer outcomes when compared to other families. These poorer outcomes often include health problems. Addiction has well-known effects on health, and the specific manifestations of these problems for parents have been documented for years in child protection casework. However, what has been less investigated are the …

An Integrated Model Of Eating Disorder Risk And Protective Factors: Implications For Research And Treatment, Leslie Alison Davis-Waddle

Electronic Theses and Dissertations

Body dissatisfaction and disordered eating behaviors are significant public health concerns, especially among college-age women. Despite extensive research, the complex nature of disordered eating and its etiology has prevented a satisfactory predictive model from being developed and validated and has therefore hindered the development of effective prevention and intervention strategies. This study aimed to integrate four of the most common etiological theories into a model to predict disordered eating behaviors. Bivariate correlations and moderation analyses were conducted to determine the strength of relationships between variables and to determine whether a protective factor (i.e., self-compassion) moderates the effects of risk factors …

The Influence Of Strength In Load-Velocity Relationships In The Back Squat, Thaddeus Light

Electronic Theses and Dissertations

Load-velocity relationships may vary between people of different strength levels and across different loads. The purpose of this dissertation was to investigate how external loads influence the velocity characteristics of the back squat exercise, and the influence of strength on these variables. Healthy male students with a history of resistance training completed repetitions at specified intensities of their estimated one-repetition maximum (1RM) until they reached 1RM. Back squat 3D motion analysis was captured using four Vicon T010 cameras (Vicon Motion Systems Ltd.; Oxford, UK) and Vicon Nexus 1.8.5 software. Data were transported into R custom coding statistical analysis software (version …

Peroxide Sensing Using Nitrogen-Doped And Platinum Nanoparticle-Modified Screen-Printed Carbon Electrodes, Chidiebere Ogbu

Electronic Theses and Dissertations

Nitrogen-doped carbon materials have garnered much interest due to their abilities to behave as electrocatalysts for reactions important in energy production (oxygen reduction) and biosensing (hydrogen peroxide reduction). Here, we demonstrate fabrication methods and determine electrocatalytic properties of nitrogen-doped screen-printed carbon (N-SPCE) electrodes. Nitrogen doping of graphite was achieved through a simple soft-nitriding technique which was then used in lab-formulated screen-printing inks to prepare N-SPCEs. N-SPCEs displayed good electrocatalytic activity, reproducibility and long term stability towards the electrochemical reduction of hydrogen peroxide. N-SPCEs exhibited a wide linear range (20 µM to 5.3 mM), reasonable limit of detection of 2.5 µM, …

The Effects Of Urbanization On Avian Seed Dispersal Success Of Toxicodendron Radicans (Anacardiaceae), Aiden M. Stanley

Electronic Theses and Dissertations

Urbanization is increasing at a dramatic rate as the human population increases. While it is well-known that urbanization tends to decrease species diversity (i.e., biotic homogenization), it is not known how urbanization affects the frequency and efficiency of species interactions. Seed dispersal is a plant-animal interaction that depends on disperser feeding rate, disperser diversity, probability of seed dispersal and germination. How these factors are affected by urbanization however is unknown. In this study, we evaluate how urbanization alters these factors. Urban sites had 2x higher feeding rate and 3x higher number of disperser species. The probability of seed dispersal however …

The Distinct Expressions Of Integrins Αdβ2 And Αmβ2 Differently Regulate Macrophage Migration In 3d Matrix In Vitro And In Tissue During Inflammation, Kui Cui

Electronic Theses and Dissertations

Chronic inflammation is an essential mechanism during the development of cardiovascular and metabolic diseases. The outcome of diseases depends on the balance between the migration and accumulation of macrophages in damaged tissues. Macrophage motility is highly regulated by adhesive receptors, integrins. Namely, intermediate expression of integrin supports macrophage migration, while a high integrin density inhibits it. Our studies are focused on evaluation of the contribution of related integrins α_Dβ₂ and α_Mβ₂ to macrophage migration and development of chronic inflammation.

We found that integrin α_Dβ₂ is upregulated on M1-macrophages in vitro and …

Resilience Among Survivors Of Adverse Childhood Experiences In Appalachia, Bridget Jeter

Electronic Theses and Dissertations

The empirical investigation of adverse childhood events (ACEs) and their relationship with health and well-being outcomes in later life is increasing. Less is known about factors that may promote resilience for those who have survived such challenges, such as how resilience may be facilitated for those with ACEs residing in a marginalized region such as South Central Appalachia. Multidimensional spirituality, social support, stigma related to ACEs, and Appalachian acculturation may serve as both valid cultural factors and potential indicators of resilience. Cross-sectional, simultaneous multiple regression analysis was performed on data collected from 272 adult patients of a South Central Appalachian …

The Effects Of Branched Chained Amino Acid Supplementation On Acute Markers Of Fatigue And Performance, Joseph Walters

Electronic Theses and Dissertations

The purposes of this dissertation were to investigate the acute effects of branched-chain amino acids on psychological, physiological, and subsequent performance changes following high volume resistance training. The rationale for this study design was based on abrupt or contiguous training/ competitions that specific athletes encounter in a competitive season. This study design also sought to fill some gaps in the scientific literature concerning the efficacy of BCAAs for subjective fatigue in a resistance training paradigm. To address the purposes of this dissertation, a one-week study was conducted on resistance trained males, in which half of the subjects were randomly selected …

The Influence Of Chronic Branched-Chain Amino Acid Supplementation On Measures Of Central And Peripheral Fatigue In Training Athletes, Tara K. Whiton

Electronic Theses and Dissertations

Branched-chain amino acid (BCAA) supplementation may improve recovery from competitive endurance training by reducing symptoms of central and peripheral fatigue. The purpose of this dissertation was to provide evidence for the use of BCAAs as a training nutrition strategy in order to improve recovery from training and further drive adaptive processes to training by increasing readiness to train. Collegiate distance runners undergoing intense competitive endurance training were monitored for symptoms of muscular soreness and psychological stress through a rated soreness chart and the Daily Analysis of Life Demands for Athletes Survey (DALDA) while taking either a BCAA supplement or a …

Neonatal Intensive Care Unit Speech-Language Pathologists’ Perceptions Of Infants With Neonatal Abstinence Syndrome, Lauren Fabrize

Electronic Theses and Dissertations

Objective: The purpose of this study was to determine the characteristics, assessment, and treatment of infants with Neonatal Abstinence Syndrome (NAS) as perceived by Speech-Language Pathologists (SLP) and whether it differed from those of other Neonatal Intensive Care Unit populations.

Methods: A secure web-based questionnaire with 62 questions collected information on NAS, caseloads, treatment environment, and demographics. Twenty-six respondents initiated the survey; 42% completed most or all questions. Response analyses included descriptive and nonparametric inferential statistics.

Results: Infants with NAS were on the caseloads of 73% of respondents. The majority (79%) only saw infants with NAS and feeding problems. Primary …

Pathways To Delinquent And Sex Offending Behavior: The Role Of Childhood Adversity And Environmental Context In A Treatment Sample Of Male Adolescents, Kelcey Puszkiewicz

Electronic Theses and Dissertations

Exposure to more types of Adverse Childhood Experiences (ACEs) has been associated with a greater likelihood of general and sexual offending behaviors. However, few studies exist that consider both the impact of varied ACE exposures and community correlates on pathways to offending behaviors in adolescents who have engaged in sexually abusive behaviors. The current study examined these pathways using data collected from archival records of male adolescents (N= 285) who had received treatment for sexually abusive behavior at a youth facility. Structural equation modeling revealed a three-factor model for ACEs, which included: nonsexual abuse and neglect; household dysfunction; …

Exploring The Moderating Effect Of Maternal Scaffolding On The Temperament - Language Development Relationship, Chelsea L. Robertson

Electronic Theses and Dissertations

Many studies have examined the relationship between a child’s temperament and its effect on his or her early language development. However, few studies have investigated the detrimental effects a child’s negative affectivity may have on their language development and potential ways these effects may be limited through parental behaviors. The current study aimed to investigate if physical or verbal maternal scaffolding behaviors moderated the effect negative affect has on language development. Although it was expected that maternal encouragement of physical activity would play a moderating role in the relationship between temperament and language development, no such relationship was found. One …

Self-Compassion And Suicide Risk In Veterans: Serial Effects Of Shame, Guilt, And Ptsd, Jessica Mckinney

Electronic Theses and Dissertations

Suicide is a significant public health concern and ranks as the 10^th leading cause of death in the U.S. Veterans are at a disproportionately higher risk for suicide, due to risk factors such as exposure to trauma and its negative cognitive-emotional sequalae, such as PTSD, shame, and guilt. However, not all veterans exposed to traumatic events, or who experience shame and guilt, die by suicide, perhaps as a result of the presence of individual-level protective factors such as self-compassion. Conceptualized as self-kindness, mindfulness and common humanity, self-compassion is beneficially associated with mental and physical health, including reduced suicide risk. …

Simple Photochemical Reduction Of Carbon Dioxide To Formate, Ovuokenye Omadoko

Electronic Theses and Dissertations

There is a need to develop techniques for conversion of carbon dioxide to other useful products such as methanol, formaldehyde, formic acid, formate, methane, and hydrocarbons. Carbon dioxide can be converted into these products using different methods such as photochemical, electrochemical, thermochemical and hydrogenation by bacteria. Formate is of interest due to its wide industrial applications which include it’s use in direct liquid fuel cells, as an additive in pyrolysis vapors, a precursor for biological fuels, and it is a key intermediate in methanogenesis breaking down complex organic compounds. In this work, conversion of carbon dioxide to formate was accomplished …

Growth Scores And Team Observation Ratings For Teachers In A Northeast Tennessee School District, David A. Little

Electronic Theses and Dissertations

The purpose of this nonexperimental quantitative study was to determine if there was a relationship between the 2018 Tennessee Value-Added Assessment System (TVAAS) Growth Score given by the Tennessee Department of Education and the overall 2018 Tennessee Educator Acceleration Model (TEAM) Observation Rating for teachers in grades 3-12 in the participating public school district. Demographic variables associated with both the teacher and evaluator were considered to determine if there existed a significant difference between gender, teaching license, grade level, TEAM Certified Observer years of experience, and the growth score and overall TEAM Observation Rating. The participating public school district is …

Changes In Psychological, Morphological, And Performance Characteristics In Preparation For A National Weightlifting Competition, Donald Marsh

Electronic Theses and Dissertations

The primary aim of this study was to examine the time course of change in muscle morphology and vertical jump performance in weightlifters preparing for a national competition. The secondary aim of this study is to examine how perceived recovery and stress state corresponds with alterations in training load leading up to competition. Eleven Olympic Training Site weightlifters completed a 4-week peaking phase for a national competition. Body mass, stress and recovery psychometric measures, and unloaded/loaded (20kg) squat jump height (SJH) were measured weekly and at the competition site. Vastus lateralis cross-sectional area (CSA), muscle thickness (MT) and pennation angle …

Evaluating Fluid Fluxes From Deep-Sea Seepage Habitats, Leigha E. Peterson

Electronic Theses and Dissertations

The permanently dark deep-sea, located at oceanic water depths greater than 200 m, represents the largest potential habitat space on Earth. The physicochemical conditions of the planet’s largest biome are tightly coupled to the exchange of matter and energy from terrestrial and sea-floor end-members. In fact, global ocean and climate systems are significantly impacted by deep-sea processes. Seafloor vents and seeps appear to act as geologic exchange conduits, returning recycled materials to the hydrosphere to sustain another generation of life. Despite submarine seepage having control on global elemental cycling, it is estimated that less than 1% of the deep-sea has …

Is Touchmath An Effective Intervention For Students With Autism?, April M. Huckaby

Electronic Theses and Dissertations

The TouchMath program was created in 1976 to help students struggling with basic mathematical computations (Bullock, 2005). Although the research has found TouchMath to be an effective intervention for students in the general and special education populations, only four studies have found the program to be effective for students with Autism Spectrum Disorder (Berry, 2009; Cihak & Foust, 2008; Fletcher, Boon, & Cihak, 2010; Yıkmış, 2016). The purpose of the study was to determine how the intervention can affect accuracy and fluency for students with ASD. The study focused on single-digit plus single-digit addition problems with three participants diagnosed with …

Simulation Of A Continuum Tumor Model Using Distributed Computing., Dylan A Goodin

Electronic Theses and Dissertations

Mathematical modeling aims to provide a theoretical framework for understanding tissue dynamics and for establishing treatment response for diseased tissues, such as tumors. Previously published continuum models have successfully represented idealized two-dimensional and three-dimensional tissue for short periods of time. A recently published continuum model of cancer increases model complexity and describes three-dimensional tissue that, due to the required complexity of the geometric multigrid solver, can only be feasibly applied to millimeter-scale simulations. Furthermore, the computational cost for such models has hindered their application in the laboratory and in the clinic. With computational demands greatly outpacing current openMP-based approaches on …

Teacher Personality And Student Engagement: A Case Study, Cole Sandlin

Electronic Theses and Dissertations

The problem that drove this study was the large number of students experiencing low levels of academic engagement. This level of engagement decreases as students progress from elementary to high school. The purpose of the qualitative study was to gain a deeper understanding of specific teacher personality traits and how teachers and students in the local setting perceived those traits impacting student engagement. This qualitative holistic case study was conducted through observations of high school teachers and semistructured interviews with high school teachers and students. The sample population included high school students and teachers in a local high school in …

Arachidin 3 Modulation Of Lipid Metabolism In Rotavirus Infections, Stormey Wisdom

Electronic Theses and Dissertations

Rotavirus (RV) can cause severe and deadly gastroenteritis in young children, infants, and immunocompromised individuals. Previous studies have shown that arachidin 3 (A3) inhibits RV replication, and that RV replication is dependent on the presence of lipids. This study investigated the alteration of lipid metabolism by A3 in RV infected HT29.f8 cells. A decrease in the RV regulation of lipid biosynthesis genes was observed with the addition of A3 using qRT-PCR. Also, immunofluorescent and histochemical staining for neutral fats, a major component of cellular lipid droplets, revealed an increased accumulation with both RV and RV+A3 when compared to no virus …

Developing Resilience Training For The Healthcare Employee In A Rural Medical Center, Sandra E. Gothard

Electronic Theses and Dissertations

In the clinical setting and in their personal lives, health professionals are confronted with many stressors that impact their time and the clarity of their role. Stressors are emotional, moral, or spiritual in nature as a result of exposure to suffering and death. There are often occupational stressors, such as reduced social support, excessive workload, or a prolonged misalignment among personal needs, individual values, and the work role. As a result of these challenges, health care employees need to create coping skills when stressors and demands become hindrances to personal well-being and their professional ability to care for others. Developing …

Postsecondary Students’ Perceptions Toward Accepting And Understanding Biological Evolution: A Qualitative Metasynthesis, Erin Childress

Electronic Theses and Dissertations

The purpose of this qualitative meta-synthesis is the exploration and analysis of postsecondary students’ perceptions towards accepting and understanding evolution. Evolution as a process and theory is a foundation for understanding the biological sciences. Within the scientific and educational communities, evolution is a central theme tying the fabric of the biological sciences together. A three-tiered analysis was used to determine postsecondary students’ perceptions towards accepting and understanding evolution. This meta-synthesis analysis was guided by the following research questions: (1) What perceived conflicts do post-secondary students face when learning about evolution? and (2) Based on post-secondary students’ perspectives, how do personal …

Molecular Characterization Of The Putative Nucleotide- Binding And Hydrolyzing Activities Of The Ellis Van Creveld (Evc) Protein, Giang Nguyen

Electronic Theses and Dissertations

Ellis–van Creveld (EvC) syndrome is a rare autosomal recessive disorder that is characterized by short-limb dwarfism, extra fingers or toes, malformed teeth and nails, and congenital heart defects. Mutations in two genes, EVC and EVC2, which are located on chromosome 4, have been shown to be responsible for EvC syndrome. The protein products of these genes, EVC and EVC2 physically interact and together, they are tethered to the base of the primary cilium of cells. This multi-protein assembly positively regulates the Sonic and Indian hedgehog signaling pathways by promoting downstream processes after activation of the smoothened receptor (Smo), by the …