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Revelations In The Blood: Determination Of Biomarkers And Analytes, Jeremiah Jamrom
Revelations In The Blood: Determination Of Biomarkers And Analytes, Jeremiah Jamrom
Electronic Theses and Dissertations
Changes in body chemistry have long been utilized as a tool for medical diagnosis. Whether blood panel, toxin level, antigen testing or any of the myriad other tests performed annually, searching for molecules which confer health status is a long-established practice. These tests give physicians more information and allow them to make a more accurate diagnosis, from which appropriate treatment can stem. Awareness of, and the subsequent search for, biomarkers for disease states have grown substantially in the past years as analytical, biochemical, and instrumental techniques have become more precises and powerful. In an era of increasingly personalized medicine, novel …
Speciated Isotope Dilution Mass Spectrometry In Combination With Thor’S Hammer Metaspike Technology For The Assessment Of Low-Level Biomarkers For Human Health Evaluation, Ashley Ebert
Electronic Theses and Dissertations
Our research team has been focused on quantifying important biological analytes in human blood samples to delineate the biochemical processes underlying certain states of dysfunction and evaluate treatment efficacy. These efforts have been guided by laboratory measurements with the input of medical experts. Speciated isotope dilution mass spectrometry (SIDMS) explained in detail in EPA Method 6800 has been successfully applied in the quantification of an important biomarker analyte for immune function and detoxification processes: glutathione. Past research has proven the molecule a biomarker for autism spectrum disorder (ASD) in the form of the reduced/oxidized glutathione (GSH/GSSG) ratio, and literature has …
The Relationship Between Urban Green Space Perception And Use Within The Adolescent Population: A Focused Ethnography, Rachel Lyons
The Relationship Between Urban Green Space Perception And Use Within The Adolescent Population: A Focused Ethnography, Rachel Lyons
Electronic Theses and Dissertations
Introduction: Greenspace is beneficial for improving adolescent mental health, yet we still do not understand the connection between the built environment and subjective mental wellbeing. We also lack understanding of how this population uses greenspace and how they feel when in it. Purpose: The purpose of this qualitative study was threefold: to understand why adolescents use greenspace, to identify how they use greenspace, and to explore how they feel when they are in greenspace. Methods: participated in a focused ethnography that utilized auto photography for photo elicitation. Braun and Clark's (2006), six phases of thematic analysis were used to guide …
Distribution And Soil Characteristics Associated With The Neches River Rose-Mallow (Hibiscus Dasycalyx), Morgan E. Langlinais
Distribution And Soil Characteristics Associated With The Neches River Rose-Mallow (Hibiscus Dasycalyx), Morgan E. Langlinais
Electronic Theses and Dissertations
The Neches River rose-mallow, Hibiscus dasycalyx, is a rare herbaceous perennial endemic to East Texas. The threatened, obligate wetland species is currently at risk of being lost in the wild due to habitat loss, encroachment of nonnative and native plant species, and hybridization with two other Hibiscus congeners, Hibiscus laevis and Hibiscus moscheutos. Known locations of each of the three Hibiscus species were used to measure select soil characteristics and habitat variables associated with each of the rose-mallows. H. dasycalyx was concluded to be associated with more fertile soils and slightly more canopy coverage than H. laevis and …
Next-Generation Sequencing Shows Increasing Temperatures Affect Stream Fungal Communities, Regan Hodgson
Next-Generation Sequencing Shows Increasing Temperatures Affect Stream Fungal Communities, Regan Hodgson
Electronic Theses and Dissertations
Under climate change predictions, surface air temperature is expected to continue to rise and will likely affect functioning of stream ecosystems and microbial communities. Aquatic hyphomycetes are key fungal decomposers of plant litter in headwater streams controlling carbon, energy, and nutrient flows to higher trophic levels. This project addressed the effects of temperature on stream fungal communities in two recently conducted experiments (microcosm and streamside channel studies) using Illumina sequencing. The objectives were to determine (1) if temperature affects community structure of stream fungi based on relative abundances of ITS2 rDNA sequences, (2) if the effects of temperature on aquatic …
Abundance And Growth Of Juvenile Atlantic Sturgeon In The Edisto River, Sc, Molly Katherine Takacs
Abundance And Growth Of Juvenile Atlantic Sturgeon In The Edisto River, Sc, Molly Katherine Takacs
Electronic Theses and Dissertations
Information on abundance and body growth are important for understanding the status and health of populations, and this information can be used to guide and monitor conservation and recovery efforts for species of conservation concern. The Atlantic Sturgeon (Acipenser oxyrinchus oxyrinchus) is an anadromous fish native to the Atlantic coast of the United States and Canada. Due to overfishing and habitat degradation, population levels declined, and Atlantic Sturgeon were placed on the Endangered Species list in 2012. Although there is substantial research on adult Atlantic Sturgeon, knowledge about their early life history is lacking. The objective of this study were …
Neurocognitive And Momentary Assessment Of Fear-Based Mechanisms Underlying The Transition From Partial To Full Remission In Anorexia Nervosa., Caroline Christian
Neurocognitive And Momentary Assessment Of Fear-Based Mechanisms Underlying The Transition From Partial To Full Remission In Anorexia Nervosa., Caroline Christian
Electronic Theses and Dissertations
Background. Anorexia nervosa (AN) is a serious psychiatric illness, with high mortality, chronicity, and societal costs. Many individuals with AN that are weight restored do not to fully remit from cognitive-affective symptoms (e.g., fear of weight gain) maintaining the disorder, contributing to the poor outcomes. This critical stage of AN is called partial remission. AN is characterized by high levels of fear and avoidance, which may prevent full remission; however, the role of fear-based mechanisms (i.e., fear approach; avoidance) in partially-remitted AN remains untested. Methods. In the current study (N = 55 partially-remitted AN), participants completed two behavioral paradigms …
Examining Effective And Realized Healthcare Access In The West South Central Division., Katherine Cameron
Examining Effective And Realized Healthcare Access In The West South Central Division., Katherine Cameron
Electronic Theses and Dissertations
This study explores realized and effective access domains outlined by Andersen’s Behavioral Model of Health Services Use. Exploring other domains of access is critical to understanding how the healthcare system functions (Kirby & Yabroff, 2020). This study evaluates rural-urban and racial/ethnic differences in access to care using two underresearched domains of healthcare access. It also allows for exploring access disparities within rural communities amongst minority populations. This dissertation is divided into five chapters. Chapter One provides an overview of health disparities and inequities and a brief overview of the study. Chapter Two details a high-level history of racism and its …
Death Of A Bacterium: Exploring The Inhibition Of Staphylococcus Aureus By Burkholderia Cenocepacia., Tiffany Brandt
Death Of A Bacterium: Exploring The Inhibition Of Staphylococcus Aureus By Burkholderia Cenocepacia., Tiffany Brandt
Electronic Theses and Dissertations
Antimicrobial resistance is a phenomenon of increasing concern as antimicrobial overuse and misuse eliminate current therapeutic options, ushering society into a post-antimicrobial era. Antibiotic discovery and synthesis efforts are urgently needed to counter the increasing burden of antimicrobial resistance. Staphylococcus aureus is a causative agent of a variety of clinical manifestations including bacteremia, endocarditis, soft tissue infection, osteomyelitis, and device-related infections. S. aureus infection presents additional treatment challenges due to its capacity for biofilm formation, which is a mode of growth that confers protection from antibiotics and physical elimination, and the emergence of antibiotic resistant strains, including methicillin-resistant S. aureus …
Metabolic Foundations Of Exercise-Induced Cardiac Growth., Kyle Fulghum
Metabolic Foundations Of Exercise-Induced Cardiac Growth., Kyle Fulghum
Electronic Theses and Dissertations
Regular aerobic exercise promotes physiological cardiac growth, which is an adaptive response thought to enable the heart to meet higher physical demands. Cardiac growth involves coordination of catabolic and anabolic activities to support ATP generation, macromolecule biosynthesis, and myocyte hypertrophy. Although previous studies suggest that exercise-induced reductions in cardiac glycolysis are critical for physiological myocyte hypertrophy, it remains unclear how exercise influences the many interlinked pathways of metabolism that support adaptive remodeling of the heart. In this thesis project, we tested the general hypothesis that aerobic exercise promotes physiological cardiac growth by coordinating myocardial metabolism to promote glucose-supported anabolic pathway …
Re-Engineering Cardio-Oncology Testing Using Biomimetic Heart Slice Cultures., Jessica Miller
Re-Engineering Cardio-Oncology Testing Using Biomimetic Heart Slice Cultures., Jessica Miller
Electronic Theses and Dissertations
28% of drug withdrawal from the market are due to unforeseen disruptions in cardiomyocyte contractility and electrophysiology. The most commonly used platforms for drug testing are in vivo animal models and in vitro cell culture models. While both have been of paramount importance for the discovery and detection of many cardiotoxicities and mechanisms of action, they lack the ability to model an intact human myocardium. This work aims to establish cardiac tissue slices, which are 300-micron thin tissue sections taken from the left ventricular myocardium, as an alternative platform for cardio-oncology studies, specifically cardiotoxicity testing. Additionally, this work aims to …
Examining The Effects Of Npra Antagonist Anantin On Sci-Induced Ployuria., Lindsey Zipperer
Examining The Effects Of Npra Antagonist Anantin On Sci-Induced Ployuria., Lindsey Zipperer
Electronic Theses and Dissertations
Polyuria, or the over production of urine, is a prevalent condition that significantly impacts the quality of life in individuals suffering from a spinal cord injury (SCI). Post-SCI induced polyuria is thought to have been associated with altered levels of vasopressin (AVP), atrial natriuretic peptide (ANP), and natriuretic peptide receptor A (NPRA). In the present study, the function of NPRA was analyzed in the rat contusion model using anantin, an NPRA antagonist. A daily dose of either 100 µg of anantin or vehicle was administered intraperitoneally immediately following the SCI surgery and continued until termination, 4 weeks post injury (wpi). …
Hgf-Mediated C-Met Signaling In Human Corneal Epithelial Cells., Kate Tarvestad
Hgf-Mediated C-Met Signaling In Human Corneal Epithelial Cells., Kate Tarvestad
Electronic Theses and Dissertations
Vision is often regarded as the primary sense of humans. The cornea is the main refractive tissue that permits light through to the retina, allowing a clear image. When the cornea sustains damage, it opens a pathway for infection and blindness through fibrotic processes. Healing the corneal tissue is critical for vision restoration and pain alleviation. Growth factors and their cognate receptors are currently under investigation as tools to restore proper corneal physiology. We hypothesize that manipulating the hepatocyte growth factor (HGF)/ c-Met signaling pathway is one route to promote quality corneal healing. This thesis investigates the signaling, trafficking, and …
A Comparison Of The Mental Health Of Police Officers And Correctional Officers In Rural Appalachia, Sierra Thomas
A Comparison Of The Mental Health Of Police Officers And Correctional Officers In Rural Appalachia, Sierra Thomas
Electronic Theses and Dissertations
The purpose of this study was to explore perceptions of mental health among police officers and correctional officers within rural Appalachia. The main goal of this research was to better understand how the occupational demands of working in the criminal justice field can impact one’s mental health over time. Several research questions were explored, including the prevalence of various mental health problems, associated stressors, the structure of support among officers, and the perceptions of mental health treatment services. Data were gathered through semi-structured interviews with 21 police and correctional officers located in rural Appalachia. Results provided a better understanding of …
Effects Of Prescribed Fire Regimes On White-Tailed Deer (Odocoileus Virginianus) Browse, Forage, And Nutrient Availability In The Pineywoods Ecoregion Of Texas, Wyatt L. Bagwell
Effects Of Prescribed Fire Regimes On White-Tailed Deer (Odocoileus Virginianus) Browse, Forage, And Nutrient Availability In The Pineywoods Ecoregion Of Texas, Wyatt L. Bagwell
Electronic Theses and Dissertations
Fire is a management tool for white-tailed deer (Odocoileus virginianus) habitat, influencing browse availability, herbaceous production, and nutrient content. In 2020 and 2021, this study utilized 46 plots across East Texas to assess these habitat components. A stem count index survey method was used to assess browse utilization prior to April in both years, and preferred browse species were clipped and analyzed for nutrient availability, and herbaceous production was also measured. Additionally, white-tailed deer population data, browse survey data, and recent fire history were obtained from five different wildlife management areas to examine their relationship. Deer had preferences …
All-Day Prekindergarten: Closing The Achievement Gap In Literacy, Theodore Clevenger Iv
All-Day Prekindergarten: Closing The Achievement Gap In Literacy, Theodore Clevenger Iv
Electronic Theses and Dissertations
The purpose of this quantitative causal-comparative research study was to compare the impact that half-day and full-day prekindergarten had on economically disadvantaged students on Grade 3 State of Texas Assessments of Academic Readiness (STAAR) Reading test results. Data from students who previously attended a half-day and full-day prekindergarten program were analyzed to see which program had a greater impact on STAAR reading scores at the conclusion of third grade. The comparison included two urban school districts with an economically disadvantaged threshold of 70% or greater. Descriptive statistics to obtain frequencies were used to create data that showed how students performed …
Improving Hypertension Self Management In African American Men, Elizabeth C. Felix
Improving Hypertension Self Management In African American Men, Elizabeth C. Felix
Electronic Theses and Dissertations
Hypertension is a cardiopulmonary condition linked to genetic, environmental, and social factors. Left untreated, it can lead to fatal conditions such as heart disease. In the United States, African Americans experience disproportionately high rates of hypertension, earlier onset of symptoms, greater severity, and higher related mortality rates. African Americans also statistically have more difficulty self-managing their hypertension than other demographics. The aim of this project was (a) to identify, define, and measure perceived barriers to self-management of hypertension among African American men and (b) to target these barriers and increase patient engagement through personalized intervention plans. The researcher recruited 24 …
Novel Signal Sequences And Fusion Partners For Paratransgenesis In Asaia, Christina Grogan
Novel Signal Sequences And Fusion Partners For Paratransgenesis In Asaia, Christina Grogan
Electronic Theses and Dissertations
Mosquitoes transmit many pathogens that cause human disease. One such disease, malaria, is caused by parasites in the genus Plasmodium, infecting over 200 million people and killing over 600,000 per year. Current strategies to control vector-transmitted diseases are increasingly undermined by mosquito and pathogen resistance. Research has turned to additional and novel methods of control, such as altering the microbiota of the vectors. In this method, called paratransgenesis, symbiotic bacteria are genetically modified to affect the mosquito’s phenotype by engineering them to deliver antiplasmodial molecules into the midgut to kill parasites. These molecules must be released by the …
Translating Particulate Hexavalent Chromium-Induced Chromosome Instability From Human Lung Cells To Experimental Animals, Human Lung Tumors And Whale Cells., Haiyan Lu
Electronic Theses and Dissertations
Lung cancer is a major human health problem. While smoking is the most well-known cause of lung cancer, people who never smoked develop the disease. Understanding how non-tobacco environmental carcinogens cause lung cancer is key to combating this disease. Hexavalent chromium [Cr(VI)] is a well-established human lung carcinogen, but its carcinogenic mechanism is uncertain. Chromosome instability (CIN) is a hallmark of lung cancer and a major factor in Cr(VI)-induced lung cancer. Studies in human lung cells show Cr(VI) induces DNA double strand breaks and suppresses homologous recombination (HR) repair by targeting RAD51, resulting in CIN. We translated these outcomes to …
Determination Of The Functional Role Of Rab-Ggt In Physcomitrium Patens., Hyun Jin Jung
Determination Of The Functional Role Of Rab-Ggt In Physcomitrium Patens., Hyun Jin Jung
Electronic Theses and Dissertations
Protein prenylation, a common lipid post-translational modification, is required for growth and development in eukaryotes. Rab geranylgeranylation involves the addition of one or two 20-carbon geranylgeranyl moieties to Rab-GTPase target proteins, which regulate intracellular vesicle trafficking. The reaction is carried out by heterodimeric Rab geranylgeranyltransferase (Rab-GGT), which is composed of two associated α- and β-subunits, with the assistance of an additional protein called Rab escort protein (REP). Loss of function of the Rab-GGT α subunit RGTA1 has not been reported in any plant. While knockout of either of the two β subunits RGTB1 or RGTB2 results in …
Characterization Of Microbotryum Lychnidis-Dioicae Secreted Effector Proteins That Manipulate Its Host Plant, Silene Latifolia., Ming-Chang Tsai
Characterization Of Microbotryum Lychnidis-Dioicae Secreted Effector Proteins That Manipulate Its Host Plant, Silene Latifolia., Ming-Chang Tsai
Electronic Theses and Dissertations
The smut fungal species Microbotryum lychnidis-dioicae is an obligate phytopathogen colonizing the plant host, Silene latifolia. A significant feature of M. lychnidis-dioicae infection is that the fungus can replace pollen on the anthers of susceptible host plants with fungal teliospores, thus earning the fungus the name: anther smut disease of flowers. The fungus synthesizes and secretes effector proteins into the cells of the plant host during infection, and the protein-protein interactions may interfere with and modify metabolism, plant development, and gene expression of the host to allow fungal colonization. Two potential fungal effector proteins, MVLG_06175 and MVLG_05122, were identified …
Continuance Intention Of Using Online Food Delivery Applications: Customers With Food Allergies, Jeongyeon Ahn
Continuance Intention Of Using Online Food Delivery Applications: Customers With Food Allergies, Jeongyeon Ahn
Electronic Theses and Dissertations
This study investigated the predictors of the continuance intention of using online food delivery applications (OFD apps) in the context of U.S. consumers with food allergies. Extending the UTAUT2 model with two context-specific variables, perceived risk, and trust, the current study tested the relationships in the extended model. A self-administered online survey was conducted to collect a sample of 293 U.S. OFD app users with food allergies and the extended UTAUT2 model was tested using a three-step hierarchical multiple regression analysis. The results of the study revealed that habit and trust were the only significant constructs that predict the continuance …
Community Structure Analysis Of Turtles With Application To The Early Pliocene Gray Fossil Site, Julian Conley
Community Structure Analysis Of Turtles With Application To The Early Pliocene Gray Fossil Site, Julian Conley
Electronic Theses and Dissertations
Turtles are important components of ecosystems around the world, with diverse ecological niches and adaptations. However, there are few detailed studies of how turtle community structure reflects local environments. This project applied techniques of community structure analysis to sites across the United States to infer past ecosystem and environmental conditions of the early Pliocene Gray Fossil Site (GFS) in northeastern Tennessee based on the ancient turtle community. Results indicate extant turtle community structure closely reflects environmental conditions, and that ancient turtle communities can be used to infer climate and habitat conditions of past ecosystems. Application to the GFS turtle community …
Tobacco-Derived Aldehydes, Platelets, And Thrombogenicity: Role Of Transient Receptor Potential Ankyrin-1., Andre D. Richardson
Tobacco-Derived Aldehydes, Platelets, And Thrombogenicity: Role Of Transient Receptor Potential Ankyrin-1., Andre D. Richardson
Electronic Theses and Dissertations
Cardiovascular disease (CVD) remains the world’s leading cause of morbidity and mortality. Conventional smoking of tobacco cigarettes is the number one risk factor for onset of CVD. Active cigarette users as well as those individuals who are exposed to second-hand cigarette smoke are at increased risk of CVD. Studies have shown that conventional smoking significantly increases a user’s risk of thrombosis, atherosclerosis, peripheral arterial disease, and coronary artery disease. Any of these diseases may trigger myocardial infarctions, stroke, or hypertension that may lead to cardiovascular morbidity and mortality. New and emerging electronic nicotine delivery systems (ENDS), such as electronic cigarettes …
The Effects Of Interactive Object Provisioning On Corticosterone, Stress-Related Behaviors, And Cognition In Juvenile And Adult Zebra Finches (Taeniopygia Guttata), Lucas West
Electronic Theses and Dissertations
Identification and reduction of unnecessary stress in lab animals is important for welfare, and for scientific validity. Providing animals with infrastructure and objects that encourage exercise and species-typical manipulative behaviors can help reduce stress by meeting physical and psychological needs. The effects of such "environmental enrichment" (EE) have been heavily studied in both zoo animals "where it has been shown to reduce maladaptive behaviors" and in lab animals such as rodents, where it clearly reduces stress and anxiety, dampens reactions to stressful events, and increases cognition and the volume of related brain regions. While there is some evidence that EE …
Organizational Green Claims And Consumer Green Perceptions In The Foodservice Industry, Jangwoo Jo
Organizational Green Claims And Consumer Green Perceptions In The Foodservice Industry, Jangwoo Jo
Electronic Theses and Dissertations
Due to the ever-growing consumer demands and global pressures for more sustainable business operations, corporations disclose their sustainable practices, products, and services to enhance their environmental image. Consumer green perceptions resulting from those organizational green claims have not been the focus of foodservice research. The application of green performance dimensionality of the foodservice domain identified that organizational green claims regarding food, environment and administration increase consumer green confusion and green trust at the same time. Based on the results of CFA, SEM, and multigroup analyses, this study suggests implications both theoretical and practical.
Preparation And Characterization Of 3d Printed Bioadhesive Delivery Systems, Mohammed Yahya Alyahya
Preparation And Characterization Of 3d Printed Bioadhesive Delivery Systems, Mohammed Yahya Alyahya
Electronic Theses and Dissertations
Drug delivery systems are evolving as pharmaceutical excipients and the manufacturing technologies evolve. Recently, 3D printing was introduced as a promising and versatile manufacturing technology of solid dosage forms from liquid, semi-solid, and solid intermediates. This work presents two examples of employing hot melt extrusion and fused deposition 3D printing technology to fabricate bioadhesive delivery systems loaded with miconazole. The first project shows a successful fabrication of four intraoral mucoadhesive film formulations, the formulations consist of different ratios of Eudragit RS, Hydroxypropyl cellulose EF (HPC EF), and Hypromellose HME 15LV. After the HME and 3DP fabrication of the films, the …
Exploratory Analysis Of Effects Of Data On Adversarial Perturbations, Hamza Ali Zafar
Exploratory Analysis Of Effects Of Data On Adversarial Perturbations, Hamza Ali Zafar
Electronic Theses and Dissertations
In recent years, the amount of data being produced has increased tremendously. This data has allowed us to create and train machine learning models that are being used nowadays. Though these models have proven to be very effective in classification and regression problems, they do have a vulnerability. This vulnerability is exploited by adding adversarial perturbations to the original data or in the deployed model. These adversarial attacks are done by making minute changes to the data to confuse the machine learning model. This can lead to models’ misrepresentation and drop the accuracy. For the span of this paper, I …
Adverse Childhood Experiences And Quality Of Life: A Mediating Role Of Physical Activity And Executive Function, Loni Parrish
Adverse Childhood Experiences And Quality Of Life: A Mediating Role Of Physical Activity And Executive Function, Loni Parrish
Electronic Theses and Dissertations
ACEs have been associated with heightened risk for a range of chronic health problems, substance use, and cognition in adulthood (Center for Disease Control (CDC), 2019; Hinojosa et al., 2017). One potential protective factor is physical activity (McEwen, 2016; Wu et al., 2013). Physical activity is associated with sustaining overall health, improving mental health by reducing symptoms of depression and anxiety (Murri et al., 2019; Sharma et al., 2006; Tasci et al., 2019), and maintaining a healthy body weight and BMI (WHO, 2021). Therefore, this study examined whether barriers to physical activity, physical activity levels, and executive outcomes serve as …
The Paleoecology Of High-Elevation Bison In The Greater Yellowstone Ecosystem And Implications For Modern Bison Conservation, Darian Bouvier
The Paleoecology Of High-Elevation Bison In The Greater Yellowstone Ecosystem And Implications For Modern Bison Conservation, Darian Bouvier
Electronic Theses and Dissertations
The national mammal of the United States, the American Bison (Bison bison) was once nearly extinct. Populations have recovered to the degree that thousands roam the Great Plains today. Due to their large numbers and body size, this species has an oversized impact on the ecological communities where it lives and is considered a keystone herbivore in modern North American grasslands. This study explores the detailed, seasonally resolved, paleoecology of seven bison from the Greater Yellowstone Ecosystem during the Late Holocene through stable isotope analyses and species niche modeling. Isotopic analyses of δ13C, δ15N, …