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Food Availability, Including Traditional Foods, In Grocery And Convenience Stores In 6 High Obese Counties In South Dakota, Including Native American Reservations, Francesca Willard Jan 2018

Food Availability, Including Traditional Foods, In Grocery And Convenience Stores In 6 High Obese Counties In South Dakota, Including Native American Reservations, Francesca Willard

Electronic Theses and Dissertations

Native American populations are disproportionately affected by obesity and its associated chronic diseases. Rural areas are also disproportionately affected by obesity and face significant challenges related to their food environment, food security, and food availability. The combination of these barriers make it challenging for rural, NA reservations to combat these health disparities and have opportunities to make healthy food choices. The lack of access to healthy and traditional foods in these areas is of great concern. The purpose of this study is to determine the food availability, including traditional foods, in grocery and convenience stores in six high obese counties …

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Characterization Of Anthocyanins In Aronia, Theodore Mohr Jan 2018

Characterization Of Anthocyanins In Aronia, Theodore Mohr

Electronic Theses and Dissertations

Anthocyanins function as antioxidants and offer numerous other health benefits, such as anti-atherogenic and anti-inflammation protection, and may aid in the prevention of degenerative diseases. Aronia berries, uniquely high in anthocyanins, have a great potential in improving human wellness. The objectives of this study were: (1) to develop a rapid and reproducible analytical method for estimating the anthocyanin amount in Aronia berries; and (2) to determine the effect of growing conditions on anthocyanin concentration. A standard curve method was established based on anthocyanin absorbance in the visible range. The results reveal that the developed protocol could yield rapid and reproducible …

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Enhancing The Nutritive Value Of Corn Ddgs For Pigs, Casey Zangaro Jan 2018

Enhancing The Nutritive Value Of Corn Ddgs For Pigs, Casey Zangaro

Electronic Theses and Dissertations

Corn dried distillers’ grains with solubles (DDGS) can be a good alternative feedstuff to the traditional corn soybean meal diets since it has high fat and protein content. However, it has a high fiber content, which is not well digested by pigs and can reduce nutrient utilization by encapsulation. The nutritive value of fibrous feedstuffs like DDGS can be improved by supplementation with fiber-degrading enzymes. However, fiber-degrading enzymes have not been effective in improving digestibility of DDGS. The overall goal of this thesis research to was to unravel why pigs poorly digest DDGS and to develop strategies that can increase …

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A Pcr/Sequencing Technique To Identify Species And Genotypes Of Haemonchus From Mixed Trichostrongyle Egg Dna Recovered From Livestock Fecal Samples, Pratiksha Khanal Jan 2018

A Pcr/Sequencing Technique To Identify Species And Genotypes Of Haemonchus From Mixed Trichostrongyle Egg Dna Recovered From Livestock Fecal Samples, Pratiksha Khanal

Electronic Theses and Dissertations

Six primer pairs, each with a pair of genus-specific and universal second internal transcribed spacer (ITS2) primers were evaluated to amplify and sequence the ITS2 region of nuclear ribosomal DNA to identify the species and genotypes of Haemonchus from livestock fecal samples containing mixtures of trichostrongyle nematode eggs. Based upon the use of universal ITS2 primers on Haemonchus adults, the ITS2 region has been shown to contain three fixed single-nucleotide differences between H. placei and H. contortus. It is clinically useful to identify the species and genotypes of Haemonchus infecting livestock, but it has not been possible in fecal …

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Evaluation Of Carinata Meal In Dairy Heifer Feeding Programs, Karla Rodriguez-Hernandez Jan 2018

Evaluation Of Carinata Meal In Dairy Heifer Feeding Programs, Karla Rodriguez-Hernandez

Electronic Theses and Dissertations

The main purpose of this body of research was to evaluate carinata meal as a feedstuff for dairy heifers and lay the foundations for its future evaluation in lactating dairy cow diets. This research focused on evaluating the taste preference of carinata meal compared with other oilseeds meals and distillers dried grains with solubles (DDGS). Despite containing glucosinolates, carinata meal was preferred similarly to canola meal, had greater preference compared to camelina meal, and less preference compared to the other meals. Next, we used a limit-feeding strategy in two different feeding studies, to determine the effect of feeding carinata meal …

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Evaluation Of Methods Used To Improve Grasslands As Ring-Necked Pheasant (Phasianus Colchicus) Brood Habitat, Mandy Orth Jan 2018

Evaluation Of Methods Used To Improve Grasslands As Ring-Necked Pheasant (Phasianus Colchicus) Brood Habitat, Mandy Orth

Electronic Theses and Dissertations

Management practices designed for upland game species often focus on nest survival and hen winter survival due to the importance of these life history stages on population vital rates. However, chick survival is an important component of gallinaceous bird population dynamics, but it is poorly understood and often tends to be overlooked. Ideal brood habitat not only provides open understory for easy movement and canopy cover for protection, but also provides an abundance of arthropod foods for chicks. It has been hypothesized that restricted movement of chicks through thick vegetation in unmanaged grasslands results in lower brood survival rates. Research …

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Ischemic Preconditioning Improves Maximal Accumulated Oxygen Deficit In Ncaa Division I Middle-Distance Runners, Emily Paull Jan 2018

Ischemic Preconditioning Improves Maximal Accumulated Oxygen Deficit In Ncaa Division I Middle-Distance Runners, Emily Paull

Electronic Theses and Dissertations

Purpose: There is an ongoing debate concerning whether ischemic preconditioning elicits consistent and meaningful exercise performance benefits. We have previously demonstrated no performance benefits of ischemic preconditioning at submaximal aerobic exercise intensities. It is likely that the beneficial effects of ischemic preconditioning on performance only involve supramaximal anaerobic exercise bouts, which elicit greater metabolic and neuromuscular stress. The aim of the study was to test the hypothesis that ischemic preconditioning improves maximal accumulated oxygen deficit (aO2D), an indicator of anaerobic capacity, in NCAA Division I middle-distance runners. Methods: A randomized sham-controlled crossover study was employed in which 10 NCAA Division …

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Nutritional Value Of Modified Novel Oilseed Meals In Teleost Diets, Tom Kasiga Jan 2018

Nutritional Value Of Modified Novel Oilseed Meals In Teleost Diets, Tom Kasiga

Electronic Theses and Dissertations

Carinata Brassica carinata and camelina Camelina sativa are being genetically modified to improve their composition for biofuel production and other agricultural applications. After oil extraction, the residual de-oiled meals (40%) in protein. However, their inclusion in animal feeds is limited to 10% because of high levels of antinutritional factors (ANFs), mainly glucosinolates (GLS), sinapine, and crude fiber that limit diet intake, nutrient utilization, and lower thyroxine production, resulting in reduced growth. The nutritional value of carinata and camelina seed meals to fish were assessed by subjecting cold pressed (CP) carinata and camelina meals to extrusion (EX), solvent extraction (SE) and …

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The Role Of Programs And Policies In Shaping The Observed School Nutrition Environment And Rural Childhood Obesity, Shadai Martin Jan 2018

The Role Of Programs And Policies In Shaping The Observed School Nutrition Environment And Rural Childhood Obesity, Shadai Martin

Electronic Theses and Dissertations

Childhood obesity is an ongoing public health concern that impacts many children in the United States. Research has shown that the prevalence of childhood obesity is higher in rural populations compared to non-rural populations. This may be attributable to differences in the school nutrition environment, particularly school meals and school wellness policy implementation. In order to design effective intervention targeted at weight management and obesity reduction, information regarding the school nutrition environment must be known. Therefore, the purpose of this research was to examine the school nutrition environment, particularly school lunch participation and weight outcomes between metro, non-metro and rural …

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Baseball In The Background: Season Ticket Holder Retention In Summer Collegiate Baseball, Susan Wallrich Jan 2018

Baseball In The Background: Season Ticket Holder Retention In Summer Collegiate Baseball, Susan Wallrich

Electronic Theses and Dissertations

The purpose of this study was to investigate season ticket purchasing motives in summer collegiate baseball, specifically in the Northwoods League. The Sport Fan Motivation Scale (Wann, Schrader, & Wilson, 1999) was used in this study to determine fan attendance motives at summer collegiate baseball games. The literature review examines fan motivation, sports as entertainment, and season-ticket holder retention. The results from this study were obtained by administering a modified version of the Sport Fan Motivation Scale (Wann et al., 1999), a 20-question, 5 point, Likert scale survey to season ticket holders of a Northwoods League team. The outcome of …

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Effects Of Enzymatically Hydrolyzed Yeast Supplementation And Supplementation Frequency On Immune Parameters, Performance, And Digestibility Among Periparturient Beef Cows And Calves, Janine Swartz Jan 2018

Effects Of Enzymatically Hydrolyzed Yeast Supplementation And Supplementation Frequency On Immune Parameters, Performance, And Digestibility Among Periparturient Beef Cows And Calves, Janine Swartz

Electronic Theses and Dissertations

Effects of enzymatically hydrolyzed yeast (EHY) and supplementation frequency (FREQ) on immune parameters among beef cows and calves was evaluated. Eighty multiparous (parity = 4.2 ± 0.3) cows were fed a common brome hay-based diet (CP = 8.0 ± 0.17%). Cows were blocked by expected calving date and stratified by body condition score (BCS) before random assignment of treatment. Beginning 88 ± 5 d prior to parturition, cows were provided 1 kg daily or 3 kg every 3 d of a soybean hull-based supplement (CP = 34.0, % of DM) that contained 0 or 3 g/kg EHY. The daily supplement …

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Nutritional And Health Benefits Enhancement Of Wheat-Based Food Products Using Chickpea And Distiller’S Dried Grains, Waleed H.M. Alrayyes Jan 2018

Nutritional And Health Benefits Enhancement Of Wheat-Based Food Products Using Chickpea And Distiller’S Dried Grains, Waleed H.M. Alrayyes

Electronic Theses and Dissertations

The first objective of this research was to enhance nutritional, rheological, sensory profiles, and shelf life of wheat-based pita bread using chickpea (CP) and food grade distiller’s dried grains (FDDG) as fortification ingredients. CP and FDDG are both high-protein and high-fiber ingredients. Nutritional efficacy was evaluated. Dough rheology and product texture were also analyzed. Chemical, physical, and rheological properties of blends, doughs and finished products were evaluated and the results showed an increase in protein, fat, ash, and total dietary fiber with an increase of FDDG and CP in the wheat-based food formulation. Moisture content was decreased in both flour …

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Chronic Risk And Disease Management Model Using Structured Query Language And Predictive Analysis, Mamata Ojha Jan 2018

Chronic Risk And Disease Management Model Using Structured Query Language And Predictive Analysis, Mamata Ojha

Electronic Theses and Dissertations

Individuals with chronic conditions are the ones who use health care most frequently and more than 50% of top ten causes of death are chronic diseases in United States and these members always have health high risk scores. In the field of population health management, identifying high risk members is very important in terms of patient health care, disease management and cost management. Disease management program is very effective way of monitoring and preventing chronic disease and health related complications and risk management allows physicians and healthcare companies to reduce patient’s health risk, help identifying members for care/disease management along …

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Trunk Stability And Postural Stability In People With Multiple Sclerosis, Derek Tolbert Jan 2018

Trunk Stability And Postural Stability In People With Multiple Sclerosis, Derek Tolbert

Electronic Theses and Dissertations

Multiple Sclerosis is a neurological disease which affects an estimated 2.5million people worldwide. People with Multiple Sclerosis often experience high rates of falls, which have been associated with age, disability, and increased postural sway. Additionally, people with Multiple Sclerosis often exhibit muscular weakness and poor responses to perturbations. PURPOSE: To determine if trunk stability and postural control are altered among PwMS and if trunk muscle activity is correlated with postural stability. METHODS: Ten participants with a physician's diagnosis of Multiple Sclerosis (9 female, 1 male) were included in this study. Ten healthy controls were matched for age, height, weight, and …

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Perceptions Of Green Eating Behaviors On College Campuses In An Urban Vs. Rural Setting, Gabriella G. Nowicki Jan 2018

Perceptions Of Green Eating Behaviors On College Campuses In An Urban Vs. Rural Setting, Gabriella G. Nowicki

Electronic Theses and Dissertations

Background: Green Eating is a multidisciplinary approach to health in economic, public health, and environmental issues. GE has an evident impact on the quality of life in young adults, especially those on college campuses. Current evidence already supports an improved dietary intake in those who adopt GE behaviors. Objective: To determine if a university’s campus location affects first-year students’ exposure to green eating. We hypothesize that first-year students on university campuses located in urban settings are more likely to report GE awareness and practices than in rural settings.
Methods: First-year students were recruited for the GetFruved study in late summer …

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Weight Management Related Factors And Healthy Behaviors In Adults, Sumadhuri Pamarthi Jan 2018

Weight Management Related Factors And Healthy Behaviors In Adults, Sumadhuri Pamarthi

Electronic Theses and Dissertations

Obesity is an ongoing global pandemic that affects more than one in three adults in the United States. Being overweight or obese also increases the risk of chronic health conditions. Weight management does not only center on immediate weight loss but also on the proper maintenance of an appropriate body weight over the years. Methods of weight management include eating a healthy diet and meeting physical activity guidelines. Such weight management practices are important to ensure that the people suffering from obesity can decrease risks of morbidity or even mortality. Nevertheless, unhealthy weight management strategies might lead to unintended health …

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Application Of Drainage Water Management And Saturated Buffers For Conservation Drainage In South Dakota, Abhinav Sharma Jan 2018

Application Of Drainage Water Management And Saturated Buffers For Conservation Drainage In South Dakota, Abhinav Sharma

Electronic Theses and Dissertations

Edge of field practices such as drainage water management (DWM) and saturated buffers can reduce nutrient transport from croplands to surface waterbodies. DWM uses stackable weir boards in a control structure to manipulate the water table depth throughout the cropping season and reduce the amount of nutrient rich water draining out from the field. Saturated buffers, on the other hand, use a control structure to divert water draining out from the cropland to a vegetative strip via a subsurface tile installed parallel to a waterway. For the saturated buffer systems, a combination of natural denitrification, nitrogen mineralization, and plant uptake …

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Evaluating Bighorn Sheep Herd Response After Selective Removal Of Mycoplasma Ovipneumoniae Chronic Shedders, Tyler J. Garwood Jan 2018

Evaluating Bighorn Sheep Herd Response After Selective Removal Of Mycoplasma Ovipneumoniae Chronic Shedders, Tyler J. Garwood

Electronic Theses and Dissertations

Infected individuals vary in their contribution to disease persistence, and chronically infected individuals may sustain disease in a population. One disease that might persist in a population through chronically infected individuals is pneumonia in wild sheep. Mycoplasma ovipneumoniae (Mo), a pathogen of Caprinae commonly present in domestic sheep and goats, strongly correlates with pneumonia epizootics when it infects wild sheep populations. These epizootics can cause 40-100% herd mortality in an initial all-age dieoff, precipitate annual lamb mortality as high as 100% in following years, and sustain adult mortality long after initial all-age dieoffs. We conducted an experiment in the Black …

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Improving The Nutritional Characteristics Of Plant Feedstuff By-Products Using Fungal Metabolism, Jacob Zahler Jan 2018

Improving The Nutritional Characteristics Of Plant Feedstuff By-Products Using Fungal Metabolism, Jacob Zahler

Electronic Theses and Dissertations

Plant feedstuff by-products such as soy processing wastewater, guar korma meal, and sorghum hominy are very different, however, all possess a rich nutrient profile. Lessening their value is the presence of lignocellulose, plant anti-nutritional factors, and unfavorable protein profiles. Fungal conversion processes are an attractive approach to improving the value of these by-products by degrading detrimental fractions of each while simultaneously creating nutrient-rich cell mass. The aim of this research was to improve the value of each by-product for potential application in monogastric diets such as fish. Fungal organisms, both yeast and filamentous fungi, were examined for their ability to …

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Effect Of Exogenous Amylase And Protease On Ruminal Metabolism, Nutrient Digestibility, Rumen Microbiome, And Lactation Performance Of Dairy Cows Fed Freshly Ensiled Corn Silage Based Diets, Lauren Kaye Shearer Jan 2018

Effect Of Exogenous Amylase And Protease On Ruminal Metabolism, Nutrient Digestibility, Rumen Microbiome, And Lactation Performance Of Dairy Cows Fed Freshly Ensiled Corn Silage Based Diets, Lauren Kaye Shearer

Electronic Theses and Dissertations

The objective of this study was to examine the effects of feeding exogenous amylolytic and proteolytic enzymes on lactation performance of dairy cows fed freshly ensiled corn silage-based diets. We hypothesized that the addition of these enzymes would improve nutrient utilization and consequently lactation performance. Thirty-six Holstein cows [15 multiparous and 15 primiparous; DIM = 132 ± 48 and 6 cannulated (3 multiparous and 3 primiparous; DIM = 164 ± 50] were blocked by milk yield, DIM, parity, and body weights and used in a 9 wk randomized complete block design study.. Treatments were a 40% (DM basis) corn silage …

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Effectiveness Of A Low-Budget Sports Vision Training Program For Improving Batting Statistics Of An Ncaa Division I Baseball Team, Adam J. Melstrom Jan 2018

Effectiveness Of A Low-Budget Sports Vision Training Program For Improving Batting Statistics Of An Ncaa Division I Baseball Team, Adam J. Melstrom

Electronic Theses and Dissertations

Context: Sports vision training (SVT) has been increasing in popularity among many dynamic reactive sports. Dynamic reactive sports require extremely accurate detection and discrimination of visual stimuli in order to execute a precise motor response. Manual interception, locomotion, and depth judgement are found to be the most important visuomotor abilities with interceptive sports such as baseball. Baseball is a highly visually demanding sport, especially offensively. The effectiveness of the batter is dependent on his/her ability to locate, track, and swing at a projectile in ~0.2 seconds. This presents the opportunity to implement an SVT program with a baseball team as …

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Development Of Polylactic Acid (Pla)-Based Nanocomposite Films For Smart Food Packaging Applications, Zeyad Albahr Jan 2018

Development Of Polylactic Acid (Pla)-Based Nanocomposite Films For Smart Food Packaging Applications, Zeyad Albahr

Electronic Theses and Dissertations

Most of current food packaging resources are non-renewable and nonbiodegradable. The use of these materials has resulted in serious environmental issues. In recent years, the recognition of waste disposal problems and their impacts on the environment has risen the demand for packaging manufactured from renewable materials. However, there are some limitations of using biopolymers for food packaging applications. These limitations include unreliable mechanical and barrier properties. These issues may be addressed by combining biopolymers and nanotechnologies. Nanotechnologies are expected to improve the performance of biopolymer-based packaging materials for food packaging applications. Cellulose nanocrystals (CNC) has shown a potential to be …

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Localization Of Microcalcification On The Mammogram Using Deep Convolutional Neural Network, Jieun Jhang Jan 2018

Localization Of Microcalcification On The Mammogram Using Deep Convolutional Neural Network, Jieun Jhang

Electronic Theses and Dissertations

Breast cancer is the most common cancer in women worldwide, and the mammogram is the most widely used screening technique for breast cancer. To make a diagnosis in the early stage of breast cancer, the appearance of masses and microcalcifications on the mammogram are two crucial indicators. Notably, the early detection of malignant microcalcifications can facilitate the diagnosis and the treatment of breast cancer at the appropriate time. Making an accurate evaluation on microcalcifications is a timeconsuming and challenging task for the radiologists due to the small size and the low contrast of microcalcification. Compared to the background and mammogram …

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Impacts Of Diverse Crop Rotations And Integrated Crop-Livestock System On Soil Quality In South Dakota, Atilla Polat Jan 2018

Impacts Of Diverse Crop Rotations And Integrated Crop-Livestock System On Soil Quality In South Dakota, Atilla Polat

Electronic Theses and Dissertations

Crop diversification can be beneficial in improving soil quality parameters. Diverse crop rotations and integrated crop-livestock systems (ICLS), are two examples of crop diversification used in this study to assess soil quality. The objectives of this study were to: (i) assess the impacts of diverse rotations on soil quality parameters, and (ii) assess the impacts of low and high stocking density on soil quality parameters. Both of the studies were conducted separately and are presented as two different chapters. To accomplish objective (i), a long-term experiment was selected which was established near Brookings, South Dakota, USA at USDA-ARS lab in …

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Soybean Iron Deficiency Chlorosis: Quantitative Trait Locus Validation And Grain Yield Evaluation, Ahmed Charif Jan 2018

Soybean Iron Deficiency Chlorosis: Quantitative Trait Locus Validation And Grain Yield Evaluation, Ahmed Charif

Electronic Theses and Dissertations

Soybean production in most of the Northern Great Plain area has been challenged by iron deficiency chlorosis (IDC), which is a physiological problem with a plant grown in high pH, calcareous soil. Developing IDC-resistant cultivars is the best approach to meet this challenge. Currently, this approach is limited by lack of knowledge about genetic resources and mechanisms for resistance to IDC. The objectives of this research were to validate quantitative trait loci (QTL) associated with IDC and to evaluate the effect of IDC on yield in soybean cultivars. To validate the QTL, a population of 201 recombinant inbred lines, which …

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Case Report: A Novel Ocular Screening Aid For Detection Of Sport-Related Concussion In High School Athletes, Payton Monson Jan 2018

Case Report: A Novel Ocular Screening Aid For Detection Of Sport-Related Concussion In High School Athletes, Payton Monson

Electronic Theses and Dissertations

Objective: The purpose of this study was to develop, implement and determine the general effectiveness of a novel battery of ocular tests referred to as the Rapid Ocular Screening Test (ROST) that can be performed efficiently on the sideline and is based on our current understanding of the physiological effects of blunt head trauma on ocular functioning.
Background: Assessment of ocular function following a suspected head-injury has been recognized as an important adjunct to a concussion evaluation protocol; however, sideline-screening recommendations currently do not include a comprehensive set of ocular functioning tests. Three high school student athletes who suffered a …

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Evaluation Of An Essential Oil Blend And A Novel Soybean Variety As Alternatives To Preventative Antibiotic Use In Monogastrics, Samantha Tauer Jan 2018

Evaluation Of An Essential Oil Blend And A Novel Soybean Variety As Alternatives To Preventative Antibiotic Use In Monogastrics, Samantha Tauer

Electronic Theses and Dissertations

Increasing consumer pressure to minimize antibiotic use and the implementation of the Veterinary Feed Directive in 2017 has increased research on alternatives to preventative antibiotics. This thesis focuses on evaluation of Ralco’s Microfused™ Essential Oils (MEO) fed to broilers and soybean meal from low allergenicity soybeans fed to weaned pigs and their potential use as alternatives to antibiotics. Essential oils are known to have antimicrobial, antioxidant, antiparasitic, and digestive stimulant effects. Low allergenicity (LA) soybeans were bred to contain significantly lower concentrations of the following anti-nutritional factors and allergenic proteins: Kunitz trypsin inhibitor, soybean agglutinin (lectin), and P34 (Gly m …

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Stability Analysis Of Immunogenic Gliadin Accumulation In Hard Red Spring Wheat (Triticum Aestivum L.), Stacy Lindblom-Dreis Jan 2018

Stability Analysis Of Immunogenic Gliadin Accumulation In Hard Red Spring Wheat (Triticum Aestivum L.), Stacy Lindblom-Dreis

Electronic Theses and Dissertations

Misconceptions regarding plant breeding objectives have led to speculation regarding the increasing prevalence of gluten intolerance, wheat allergy, and celiac disease. It is thought that contemporary wheat cultivars accumulate more immunogenic proteins than older cultivars because of supposed genetic changes effected through plant breeding strategies. This study evaluated the stability of gliadin accumulation in 191 hard red spring wheat cultivars grown in 12 location-years. Two enzyme-linked immunosorbent assays (ELISAs) were developed. A paired t test (N = 34) failed to find a statistically significant difference between the experimental ELISAs and the commercial R5 assay measures of mean gliadin (t = …

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Urban Gardens And Nutrition In Sioux Falls, Sd, Austin Brynjulson Jan 2018

Urban Gardens And Nutrition In Sioux Falls, Sd, Austin Brynjulson

Electronic Theses and Dissertations

With the global population recently surpassing seven and a half billion, questions about feeding the population have emerged. In the past, increased demand for food was addressed through increasing intensification of land use and increasing the area of land under cultivation. Despite these efforts, food insecurity has increased for much of the population over this period, where, according to the USDA, in the U.S. 49.1 million people were food insecure in 2013. Food insecurity in this regard is the lack of access to sufficient, safe, and nutritious food at all times. While the complex global food system may be difficult …

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The Role Of Genomic Versatility In Multi-Niche Preferences Of Escherichia Coli, Gitanjali Nandakafle Jan 2018

The Role Of Genomic Versatility In Multi-Niche Preferences Of Escherichia Coli, Gitanjali Nandakafle

Electronic Theses and Dissertations

Escherichia coli strains are naturally present as either commensals or pathogens in the gastrointestinal tract of mammals and some other vertebrates. Until recently, it was assumed that E. coli are solely associated with the gut and are unable to survive outside of a host for a long period of time, the basis of its use as an indicator organism. Recent reports suggest that E. coli can become naturalized to several tropical, subtropical or temperate soils and aquatic environments, where they have been isolated repeatedly. Several studies have shown that these strains are capable of surviving and proliferating in the environment …

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