Digital Commons Network^™

Environmental Exposures And Aging., Daniel Chris Gomes

Electronic Theses and Dissertations

In recent years, research into air pollution has shown that exposure to certain components in air pollution, primarily PM2.5 can accelerate biological aging and thereby lead to increased susceptibility to multiple diseases. We hypothesize that prolonged exposure to air pollutants can result in premature aging leading to extensive tissue dysfunction and susceptibility to diseases. To examine this, we exposed mice to PM2.5 for 9, 15, and 21 days, then measured the telomere lengths, cellular senescence, and histone methylation patterns of multiple cell types. We found consistently increased telomere attrition, cellular senescence and advanced age-consistent histone methylation patterns in groups exposed …

Study Of The Effects Of Chronic Cadmium Exposure On The Pathogenesis Of Pulmonary Arterial Hypertension., Dakotah Dominique Cathey

Electronic Theses and Dissertations

We reported pulmonary artery hypertension (PAH) patients had elevated levels of Cd in both blood and urine, therefore, this study tested whether Cd directly induces PAH or facilitates PAH pathogenesis in mouse models. C57/6J mice were initially exposed to drinking water with or without 5ppm Cd for 8 weeks. Then, half the mice in both control and Cd groups were given SU5416 and hypoxia (SuHx) for 4 weeks to induce PAH, resulting in 4 subgroups: Control, Cd, PAH, and Cd+PAH. Diastolic and systolic functions of the left and right ventricles (LV, RV) were examined with echocardiography before and after PAH. …

Genetically Engineering Gingipains For High Yield Large Scale Purification., Andrew E. Fuchs

Electronic Theses and Dissertations

Porphyromonas gingivalis is a keystone oral pathogen in the progression of periodontal disease and has been implicated in the etiology of many other systemic human diseases. P. gingivalis produces primary virulence factors termed the gingipains, a group of cysteine proteases. There is a high demand for high purity gingipains for downstream in vitro studies where P.g. is implicated, both oral and systemic. Gingipain production candidate mutant P.g. strains were generated to isolate each gingipain for large scale purification and the purified gingipains characterized. Two mutants were determined not to be viable for large scale purification of gingipains, each for different …

The Interplay Between Lung Adenocarcinoma, Nutrient Availability, And The Microbiome., Alexis A. Vega

Electronic Theses and Dissertations

Lung cancer is one of the most common forms of cancer diagnosed, and has the highest lethality rate across all other forms of cancer in the U.S. While current therapeutic options include chemo-, immuno-, and radio-therapy, the benefits of caloric or nutrient restriction on cancer cells has also been investigated. Due to the many biological functions associated with methionine, many have proposed a methionine restricted diet would lead to favorable outcomes when combating cancer. Although our cells are incapable of synthesizing methionine, the bacteria found in our microbiome can. Furthermore, recent discoveries suggest an independent microbiome found within tumors that …

Quantification Of Tumor Biophysical Heterogeneity Through Mechanical And Ultrastructural Analysis., Bradley James Mahaffey

Electronic Theses and Dissertations

Glioblastoma (GBM) is a highly invasive, aggressive brain cancer that carries a median survival of 15 months. This poor prognosis is due, in part, to its resistance to standard therapeutic intervention. Recent studies have demonstrated that tumor heterogeneity plays a critical role in facilitation therapy resistance by mediating tumor adaptation through microenvironmental cues. Efforts to describe these microenvironmental differences may aid in the development of strategies to combat resistance. GBM can be separated into two distinct regions – a core and a rim, which are thought to drive specific aspects of tumor evolution. The core is proliferative as evidenced by …

Perovskite Oxide Derivatives For Enhanced Electrochemical Water Splitting And Pseudocapacitor Applications., Md. Sofiul Alom

Electronic Theses and Dissertations

The global energy landscape is at a crossroads, marked by surging demand, finite fossil fuel reserves, and escalating environmental concerns stemming from carbon emissions. To address these challenges and transition towards a sustainable energy future, this dissertation embarks on a multidisciplinary exploration of perovskite oxides and their derivatives as catalysts and materials for advanced electrochemical water splitting and pseudocapacitive energy storage. In the quest for efficient water splitting catalysts, a series of quasi-2D oxides, SrLaAl_1/2M_1/2O₄ (M = Mn, Fe, Co), was synthesized and systematically studied where the B-site comprised of both transition metals and main …

The Perpetual Hell Of Your Own Undoing., Emery Tackett

Electronic Theses and Dissertations

The Perpetual Hell of Your Own Undoing is a musical dramatic work for a chamber ensemble and two voices. In it, two souls trapped in the afterlife must struggle to regain their memories through dreams in order to figure out where they are and why they’ve been brought there. Over time, as their memories are regained, they learn that they were in fact lovers. However, by the end the realization that one of the pair killed the other threatens to tear the two apart. This musical dramatic work deals with themes of death, memory, and forgiveness. It utilizes both classical …

Investigation Of Heterocyclic Amines And N-Acetyltransferase 2 Genetic Polymorphism In The Dysregulation Of Hepatic Energy Homeostasis: A Gene-Environment Approach., Kennedy M Walls

Electronic Theses and Dissertations

Heterocyclic amines (HCAs) are mutagens generated when cooking meat for prolonged periods of time or until well-done. Recent epidemiological studies reported significant associations between dietary HCA exposure and insulin resistance and type II diabetes. However, no previous studies have examined if HCAs, independent of meat consumption, contributes to pathogenesis of insulin resistance or metabolic disease. It is well known that HCAs require hepatic bioactivation by cytochrome P450 1A2 (CYP1A2) and N-acetyltransferase 2 (NAT2). NAT2 expresses a well-defined genetic polymorphism in humans that, depending on the combination of NAT2 alleles, correlate to rapid, intermediate, or slow acetylator phenotypes that exhibit differential …

Agency In Learning And E-Learning Through The Lens Of Disability And Inclusivity., Marta Martin

Electronic Theses and Dissertations

Through the analysis of ethnographic, qualitative data collected from students and their stakeholders at the University of Louisville, this thesis examines the experiences of students with disabilities in spaces of higher education, particularly virtual learning spaces. This research has illuminated systemic gaps in disability resources and accessibility within the landscape of higher education, restricting the agency of students with disabilities and their stakeholders.

Design, Fabrication, And Integration Of Robotic Skin Sensors For Human Robot Interaction., Olalekan Olakitan Olowo

Electronic Theses and Dissertations

Enhancing physical human-robot interaction in modern robotics relies on refining the tactile perception of robot skin sensors. This research focuses on crucial aspects of the development process, including fabrication techniques, miniaturization, and integration for a more efficient collaborative human-robot interface. The fabrication process of robot skin sensors, designed to mimic human skin, is explored both within and outside cleanroom environments. An enhanced technique is presented to increase fabrication yield and create more miniaturized sensor designs with feature sizes in the tens of microns. These sensors function as piezoresistive arrays using organic polymers like PEDOT: PSS as the pressure-sensing medium. Various …

Art And Interdisciplinary Opportunities For Literacy Instruction: A Qualitative Study To Examine Educator Perceptions In The Rural Setting, Courtney Collins

Electronic Theses and Dissertations

The purpose of this qualitative phenomenological study was to examine educator perceptions of teaching art and implementing literacy and interdisciplinary strategies in rural northeast Tennessee school districts. This study explored interdisciplinary connections and opportunities to expand access to literacy acquisition in the art room. Participants included twelve educators who served as art instructors, literacy specialists, and district supervisors in rural public-school systems in Tennessee's First Congressional District. Data were collected through virtual interviews using a semi-structured format. To qualify for the study participants held positions as elementary or secondary art teachers, literacy specialists, or district supervisors in rural northeast Tennessee …

Special Education Teachers’ Emotional Well-Being And Work-Related Stressors In The School Environment, Mallory J. Laravie

Electronic Theses and Dissertations

There is little to no research regarding novice elementary special education teachers (SETs) and their emotional well-being while in the school environment. The goal of this work is to illuminate special education teacher’s voices missing in previous research. Additionally, this research examines the impact of work-related stressors on novice elementary SETs' emotional well-being in the school environment. The theoretical perspective of heuristics is used to illuminate a problem or answer a question about the lived experience of the researcher. Lazarus and Folkman’s (1984) transactional theory of stress and coping framework guided the research question development, data analysis, and presentation of …

Perceptions Of Educators On Motivational Strategies Influencing Middle School Students In Mathematics Courses, Amy Rigsby

Electronic Theses and Dissertations

This qualitative study examined educators' perceptions of motivational strategies influencing and motivating middle school students to comprehend and perform in mathematics courses. This study used interview data from 15 participants with 2 to 30 years of teaching experience. Educators may utilize this information in multiple settings within the classroom to influence and motivate students in mathematics courses.

Data collection strategies included one-on-one semi-structured middle school educator interviews. Analysis of data occurred in three phases: (a) categorization of data under the five organizational factors, (b) building the explanation in narrative form, and (c) re-examination of the data. The analysis of the …

The Experiences Of Leaders Who Transitioned To Leading A Virtual Team During The Covid-19 Pandemic: A Phenomenological Study., Jason W. Oliver

Electronic Theses and Dissertations

The COVID-19 pandemic led to organizations rapidly transitioning their workforces to virtual work settings. This sudden shift brought about many challenges to organizations and their employees. In this study, a phenomenological research approach was used to describe the lived experiences of leaders who transitioned to managing a virtual team during the COVID-19 pandemic. The study also explored how leaders adapted their leadership practices and leveraged people and technology when transitioning to managing a virtual team. The findings revealed seven themes that highlighted both benefits and challenges of virtual leadership and work. Leaders recognized the need for intentionality in their actions …

From "Smart Talk" To "Living Well": Commonplaces And Their Role In Narratives Of Rare Disease., Caitlin E. Ray

Electronic Theses and Dissertations

As healthcare becomes more complex, automated, and bureaucratic, patients often suffer from a lack of resources, agency, and visibility when seeking medical care. Rhetoric and Composition, specifically the subfield of Rhetoric of Health and Medicine (RHM), is interested in studying and intervening into such issues. One way to challenge our current understanding of healthcare is to consider how the rare disease patient experience reveals the gaps, limitations, and assumptions of illness and health. I argue here that through rare diseases, rhetoricians of health and medicine can better understand the representation, advocacy, and patient experience within healthcare, and potentially lead to …

Once Upon Online: Conversations With Professional Storytellers About Adapting From In-Person To Virtual Storytelling Performance, Leticia Pizzino

Electronic Theses and Dissertations

This study examines how professional storytellers negotiated a new storytelling stage—the videoconference platform—as they pivoted their careers during mandated shutdowns due to COVID-19. An examination of the literature reveals extremely limited research involving either professional storytellers or live virtual storytelling. After interviewing five professional storytellers, I analyzed their stories through narrative inquiry. Analysis revealed that the storytellers negotiated the limitations and affordances of Zoom and adapted their storytelling to successfully connect with their audiences. Through crafting a narrative of their stories, I was able to represent their emotions, unique experiences, and abilities to adapt to the online environment. Their stories …

School Nurses And The Sexual Health And Education Of Public-School Students In Tennessee, Amy Wake

Electronic Theses and Dissertations

Tennesseans have been experiencing increasing rates of sexually transmitted diseases since the year 2000. Those rates are highest among those 15- to 24-year-olds. At the same time, Tennessee policy requires the teaching of abstinence as the primary form of sexual health education and emphasized the use of abstinence-only curriculum. The school nurse represents an available resource in the Tennessee public schools. The purpose of the qualitative study was to explore, describe, and interpret the perceptions and experiences of public-school nurses from rural, suburban, and urban areas of Tennessee related to the sexual health and sexual health education of students. School …

Obstetric Violence And Postpartum Adjustment: Exploration Of Risk And Resilience Factors, Hope O'Neill

Electronic Theses and Dissertations

Obstetric violence includes acts of abuse, coercion, or disrespect that occur during the labor process. The present study explores how obstetric violence impacts a person’s postpartum psychosocial adjustment. This study used a subset of data (N = 339) from a larger online study, which attempted to explore multiple facets of a person’s postpartum health. The psychological constructs examined are postpartum depression and postpartum anxiety. Additional constructs assessed were protective and risk factors: self-compassion and medical mistrust. Self-compassion and medical mistrust were examined by using moderation models. Additional analyses were completed using simple regression models to explore whether obstetric violence predicts …

Covid-19 Vaccine Uptake In The Hispanic And Latinx Communities Of Northeast Tennessee, Gabriela Abrego

Electronic Theses and Dissertations

This work addresses the underlying issues surrounding COVID-19 vaccine uptake in Hispanic and Latinx communities in the Northeast region of Tennessee. A qualitative, phenomenological research approach was used to conduct interviews and data from the interviews was used to inform a survey instrument. The interview guide in the qualitative phase of the study was based on the Health Belief Model (HBM) developed by Rosenstock (Rosenstock, 1974). A quantitative approach was then used by piloting the survey instrument in the study population. Common themes found through the interviews were: previous negative experience with COVID-19 vaccination, previous negative experience as a result …

Effect Of Macrophage Expressed Α7 Nicotinic Acetylcholine Receptor (Α7nachr) On Migration Of Macrophages During Inflammation, Kasey Keever

Electronic Theses and Dissertations

Sepsis is a life-threatening condition characterized by overwhelming inflammation, resulting in organ system damage, leading to a high mortality rate. Care in the clinical setting is supportive, and there are no approved sepsis-specific treatments. In septic mice, activation of the cholinergic anti-inflammatory pathway decreases cytokine secretion by leukocytes and improves survival. The cholinergic anti-inflammatory pathway is a reflex of the parasympathetic nervous system, converging on the α7 nicotinic acetylcholine (α7nAChR) at the surface of macrophages. Signaling through the receptor blocks NF-kB activation, thus cytokine secretion. Receptor activation has other effects on macrophages, including modulating their migration to …

The Experiences Of Students During Covid-19 School Disruptions, Brandi Bullock

Electronic Theses and Dissertations

The purpose of this study was to identify the perceptions of educators with respect to student experiences between March 2020 and February 2021 related to academics, social-emotional health, and safety and security. Data collection strategies included one-on-one interviews with 12 educators in one East Tennessee school district. To qualify for the study, participants had to have taught during COVID-19 schooling disruptions and have taught either 1st, 2nd, 3rd, 4th, or 5th grade in the 2022-23 school year. Data analysis was based on Systems Theory (Laszlo & Krippner, 1998) and Change Theory (Fullan, 2020). The results revealed 5 key themes that …

Enhanced Content-Based Fake News Detection Methods With Context-Labeled News Sources, Duncan Arnfield

Electronic Theses and Dissertations

This work examined the relative effectiveness of multilayer perceptron, random forest, and multinomial naïve Bayes classifiers, trained using bag of words and term frequency-inverse dense frequency transformations of documents in the Fake News Corpus and Fake and Real News Dataset. The goal of this work was to help meet the formidable challenges posed by proliferation of fake news to society, including the erosion of public trust, disruption of social harmony, and endangerment of lives. This training included the use of context-categorized fake news in an effort to enhance the tools’ effectiveness. It was found that term frequency-inverse dense frequency provided …

Experiences Of Appalachian People With Conflicting Christian And Lgbtq+ Identities, Zacharias Smith

Electronic Theses and Dissertations

This study explores the experiences of six LGBTQ+ individuals living in Appalachia who have felt conflict between their LGBTQ+ and Christian identities. Using data from six one-on-one intensive interviews, the study examines the various kinds of conflict participants experienced, the strategies they used to manage those conflicts, and the role that Christianity played in their lives. The author found that participant conflicts were grounded in struggles to find acceptance from others while living authentically, to reconcile ideological disputes with the church, and to accept themselves as LGBTQ+ and/or Christian. Included among their various management strategies were finding accepting people and …

Crossing The Pond: The Influence Of Southern Appalachian Old-Time On Contemporary Irish Music, Amanda Morgan

Electronic Theses and Dissertations

Numerous studies examine Irish traditional music influencing old-time music, but few examine the influence of old-time on contemporary Irish. As our societies become more global, folk music travels faster and becomes more open to influence. Thes influences can be heard in the music of “Alfi” and “Lankum,” two ensembles steeped in Irish traditional music.

This study defines common musical elements of old-time and examines the use of those elements in two recordings: Alfi’s, “Jubilee” and Lankum’s, “The Old Man from Over the Sea.” Much of my data comes from interviews with Irish and American musicians and my own professional knowledge, …

The Perceptions Of Second-Year, First-Generation Minority Students Regarding The Influence Of Avid Mentoring On Students’ Retention, Nisha Mary Mathews

Electronic Theses and Dissertations

Despite the increase in diversity, institutions continue to struggle to provide, retain, and graduate students of color to the same degree as their counterparts (Banks & Dohy, 2019). The purpose of this narrative qualitative research study was to examine the perceptions of second-year, first- generation minority college students regarding the influence of the Advancement Via Individual Determination (AVID) program on their persistence and academic achievement. The study was framed by Swail’s (2004) geometric model of student persistence and achievement, aimed to identify the cognitive, social, and institutional factors contributing to student retention. The following questions guided the study: (1) What …

A Case Study Of The Political Determinant Of Health On The Public Health Crisis Of Malaria In Nigeria, Bisola M. Olumegbon

Electronic Theses and Dissertations

Abstract

Globally, there were about 229 million cases of malaria in 2022. The sub-Saharan African region accounted for 92% of the reported cases and 94% of deaths. Nigeria had the highest number of malaria cases and deaths, representing 27% of global cases. This scholarly project was a case study guided by the political determinants of health. Triangulation of data using thematic analysis was used to identify the political determinants of malaria in Nigeria and to understand how the concept of interaction contributes to the persistence of the disease. The analysis involved a deductive and inductive approach based on the literature …

Intergenerational Factors That Contribute To Millennial Church Engagement, Parker Sanderson

Electronic Theses and Dissertations

Millennial church attendance has declined since the beginning of the new millennium. Intergenerational ambivalence has been known to contribute to this phenomenon. Through this action research, the researcher sought to determine what intergenerational factors have led to millennials’ continued or discontinued attendance within churches. A phenomenological qualitative approach centered around interviews for this study was used to determine these intergenerational factors. The sample was taken from a 1,100-member church in a major Texas metropolitan area. The goal was to help church leaders decrease relational distancing and reduce ambivalent factors to increase millennial engagement in this local congregation.

Educational Robotics And Computational Thinking In Elementary School Students, Sonia D. Jordan

Electronic Theses and Dissertations

This study examined the role of educational robotics in fostering computational thinking in elementary settings, both in classrooms and extracurricular programs. Among growing concerns over K–12 students’ computational thinking deficits, the research evaluated the impact of Lego EV3 and VEX IQ platforms. Data was sourced from lesson plans, student work surveys, and teacher interviews and then subjected to thematic analysis using a qualitative approach. The participants were Texas educators engaged in robotics instruction, even though specific robotics statistics are absent in the Texas Education Agency. Instructional strategies varied from hands-on experiences to translating mathematical concepts into robotic actions. A key …

Bolting The Landscape: An Ethnography Of Yosemite As A Significant Climbing Destination, Vanessa Taylor

Electronic Theses and Dissertations

Yosemite Valley is a transformative landscape that helps to shape climbers’ identities and fosters a unique sense of community, which continually reinforces its status as a renowned and evolving climbing destination. The historical influence of Yosemite Valley on rock climbing began in the 1950s and has since defined itself as a prominent destination for climbers worldwide. This ethnographic research analyzes how climbers forge a meaningful connection with the Valley by forming a deep sense of place that intertwines with their personal identities as climbers and investigates the intricate relationship between climbers’ identities and the Yosemite landscape. This research also explores …

Enhancing Search Engine Results: A Comparative Study Of Graph And Timeline Visualizations For Semantic And Temporal Relationship Discovery, Muhammad Shahiq Qureshi

Electronic Theses and Dissertations

In today’s digital age, search engines have become indispensable tools for finding information among the corpus of billions of webpages. The standard that most search engines follow is to display search results in a list-based format arranged according to a ranking algorithm. Although this format is good for presenting the most relevant results to users, it fails to represent the underlying relations between different results. These relations, among others, can generally be of either a temporal or semantic nature. A user who wants to explore the results that are connected by those relations would have to make a manual effort …