Digital Commons Network^™

Degradation Of 23s Rrna In Azithromycin-Treated Ribonuclease Mutants Of Escherichia Coli., Jessica A. Silvers

Electronic Theses and Dissertations

Azithromycin, a macrolide antibiotic, specifically binds to the 50S ribosomal subunit of bacterial ribosomes and inhibits translation. Azithromycin also prevents 50S ribosomal subunit assembly by binding to a 50S ribosomal subunit precursor particle. When exposed to azithromycin, several ribonucleases in wild-type Escherichia coli cells degrade antibiotic-bound 50S precursor particles. Presumably, cells expressing one or more mutated ribonucleases will degrade the antibiotic-bound precursor less efficiently, resulting in increased sensitivity to the antibiotic. To test this, eight ribonucleaseûdeficient strains of Escherichia coli were grown in the presence or absence of azithromycin. Cell viability, growth rates, and protein synthesis rates were measured. Degradation …

Kinetics And Mechanism Of The Catalysis Of The Decomposition Of Hydrogen Peroxide By Schiff Base Complexes Of Copper(Ii)., Timothy Kum Beng

Electronic Theses and Dissertations

Spectroscopic studies have been used to describe the mechanism of the decomposition of hydrogen peroxide by solutions of a dimeric Cu(II) complex of a dissymetric Schiff base, [CuSALAD]₂.H₂O, and imidazole or methyl substituted imidazoles, B, which form monomeric CuSALAD.B₂ complexes, in aqueous ethanol solvent. Freezing point depression and vapor pressure lowering studies were carried out to confirm the dimeric nature of the [CuSALAD]₂.H₂O complex that had been previously reported. The stoichiometry of the [CuSALAD]₂.H₂O-imidazole equilibrium was extensively studied pointing to a 1:4 stoichiometry. The CuSALAD.B₂ adducts …

Epoch Locale., Jonathan Hounshell

Electronic Theses and Dissertations

My explorations around the world are intertwined with my excavation of the layers of art history. My discoveries about the traditions of the printmaking medium are fused with the heritage I inherit as an artist. This is what is put into my current body of work. With this supporting manuscript, I hope to inform about the technical aspects of my unique aesthetic and to entertain with the ideas behind my current body of work by making connections between my personal visions and experiences and the significance of the artists and craftsman who inspire me.

Marijuana Use By Juveniles: The Effects Of Peers, Parents Race, & Drug Abuse Resistance Education., Daniel J. Moeser

Electronic Theses and Dissertations

The purpose of this study was to analyze the factors that affect the use of marijuana by juveniles and how outside influences such as peer pressure, race, parental influence, and prevention programs such as the Drug Abuse Resistance Education (D.A.R.E.) contribute to the use of marijuana by juveniles. All of the variables used in this study came from the Gang Resistance Education and Training (G.R.E.A.T.) data collected by Esbensen and Osgood (1999). The analysis indicated that juveniles are most influenced by their peers such as friends, that African American juveniles would be less likely than Whites and Hispanics to become …

Psychosocial Antecedents Of Sex Offender Criminality And Violence., Sonia Marie Perry Mills 1965-

Electronic Theses and Dissertations

Sexual abuse and sexual violence perpetrated against women and children in the United States are social problems that need to be addressed. The United States is one of the most sexually violent societies in the world, having one of the highest rates of any industrialized country of rape and sexual violence (Stewart, 2002). Although any type of sexual victimization is problematic, sexual violence in particular can have particularly devastating effects for the victim. We urgently need to know why some adults sexually abuse and assault (Cannon, 2001). Examination of the psychosocial antecedents of sex offender criminality and violence will add …

Novel Synthesis Techniques For Nanostructures., Romaneh Jalilian 1977-

Electronic Theses and Dissertations

Crystalline beta-Ga2O3 nanowires with two distinct morphologies have been synthesized through simple physical evaporation of Te doped GaAs powder in argon atmosphere. The structure of the nanowires was characterized by SEM, TEM, XRD, EDX, and raman spectroscopy. Nanowires as long as hundreds of micrometers with diameters in the range of 20-200 nm have been produced with a high yield. Absence of Tellurium in the nanowires indicates that the growth mechanism is not VLS based. The role of Tellurium in the growth process is not clear. Substitution of sulfur in place of tellurium resulted in similar nanostructures. One of the morphologies …

The Bully-Free School Zone Character Education Program: A Study Of Impact On Five Western North Carolina Middle Schools., Richard Alan Spurling

Electronic Theses and Dissertations

In today's schools bullies have the power to be more tenacious, more vicious, and meaner than ever. We are all beginning to understand that victims of bullying are at a greater risk for depression, suicide, and hurting others through violent acts. The purpose of this qualitative study was to investigate common threads of effective Bully-Free School Zone character education programs as perceived by administrators, teachers, and parents in five middle schools in Western North Carolina. Through this strategic inquiry, I attempted to determine if the views of administrators, teachers, and parents were consistent with published research on anti-bullying programs.

Specifically, …

Genome-Wide Analysis Of Gene Expression Changes In Drosophila Melanogaster With Variable Sensitivity To Mptp, Xinyu Chen

Electronic Theses and Dissertations

1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treated mice and monkeys have been widely used as models for the study of Parkinson’s Disease (PD). The similarities between the toxic action of MPTP in PD animal models and the occurrence of dopamine (DA) neuron degeneration in human mutant a-synuclein transgenic fruit fly (Drosophila melanogaster) suggested that the fruit fly may share the same toxic response to MPTP as primates and planaria. We have hypothesized that relative sensitivity to MPTP in the fruit fly may represent a correlate to genetic predisposition to developing PD in human. Using the MPTP survival test for selection of insensitive flies and …

Authentic Versus Tourist : Reexamining A Collection Of West African Art In Light Of New Debates And Old Ambiguities, Sarah Joy Walker

Electronic Theses and Dissertations

The issues in defining, describing, and ultimately displaying “authentic” West African traditional art in the museum environment are often wrought with arbitrary distinctions concerning aesthetic value, artistic intention, and function in a ritual atmosphere. I examine a West African art collection, the Fulton-Meyer Collection, at the University of Mississippi Museums, and identify these works of art in terms of contemporary criteria found in both ethnographic and art history texts for specific tribal and ethnic affinities. I compare works deemed “authentic” and those that meet the tourist art criteria. I explore the reasons for these distinctions, and apply the contemporary studies …

The Interrelationships Of Parent Rural Values, Parent Religiosity, Parent Involvement, And Student Outcomes In A Small, Southern, Rural Middle School., E. Carolyn Tucker 1949-

Electronic Theses and Dissertations

This dissertation is a case study of 109 students and their parents from a small rural, middle school community in Western Kentucky. It seeks to determine the interrelationships between conservative Protestant Parent Values (Parent Rural Values and Parent Religiosity), Parent Involvement, and Student Outcomes. For the mixed-methods study, mail-administered Parent and Student Surveys provide quantitative data. Interviews, document analysis, and participant-observer field notes are utilized to supplement statistical findings. Ten Research Questions reflect Demographic Factors (Socioeconomic Status, Family Structure, and Specifically Designed Instruction), Independent Variables (Parent Values--Rural Values and Parent Religiosity), Mediating Factor (Parent Involvement), and the Dependent Variable (Student …

The Role Of Nuclear Factors Of Activated T-Cells (Nfat) In Neuronal Death., Agata M. Habas 1976-

Electronic Theses and Dissertations

Nuclear Factor of Activated T-cells (NFAT) is a calcium-responsive transcription factor that orchestrates immune response and cardiovascular development. NFAT is also present in neurons and glia from the Central Nervous System (CNS) where it participates in calcium signaling. In physiological conditions, neuronal NFAT may contribute to the plasticity-related transcriptional regulation. On the other hand, excessive activation of calcium signaling and the subsequent overactivation of NFAT may contribute to neuronal death. To explore the possibility of NFAT-involvement in neuronal death I have determined which components of NFAT signaling are expressed in cultured rat hippocampal or cortical neurons. I found the presence …

Correlating Technology Surveys And Third- And Fifth-Grade Proficiency Levels In Math And Reading Throughout Tennessee., Gary L. Lilly

Electronic Theses and Dissertations

This study used two different sets of data collected through two distinct means during the 2002-2003 school year. To fulfill the federal accountability requirements related to the distribution of monies known as EdTech (2002), the Tennessee State Department of Education required every school within districts that accepted the formula EdTech funds to complete an online survey called the EdTech Tennessee Online Technology Evaluation or E-TOTE. The E-TOTE survey collected information on a variety of topics related to educational technology including questions about teaching and learning, educators' preparation and development, infrastructure, and equipment counts. The other set of data came from …

Middle And High School Principals' Knowledge Of Discipline Provisions Of The Individuals With Disabilities Education Act In The Upper East Tennessee Region., Ginger R. Woods

Electronic Theses and Dissertations

The purpose of this study was to determine school principals’ knowledge of discipline provisions of The Individuals with Disabilities Education Act Amendments of 1997 and to determine what additional training opportunities may be needed to ensure that school systems in the upper East Tennessee region are in compliance under IDEA. As instructional leaders, it is imperative that principals understand their responsibilities and the importance of adhering to the legal obligations under IDEA. Principals who do not comply with the act not only deny students the education to which they are entitled but also expose the school system to costly litigation. …

Papain: A Novel Urine Adulterant., David Burrows

Electronic Theses and Dissertations

The estimated number of employees in the United Stated screened annually for illicit drugs is approximately 20 million, with marijuana being the most frequently abused drug. Urine adulterants provide an opportunity for illicit drug users to obtain a false negative result on commonly used primary drug screening methods such as the Fluorescence Polarized Immunoassay (FPIA) technique. Typical chemical adulterants such as nitrites are easily detected or render the urine specimen invalid as defined in the proposed federal guidelines for specimen validity testing based on creatinine, specific gravity and pH. Papain is a cysteine protease with intrinsic ester hydrolysis capability. The …

Risk Perceptions Of Adults In The Town Of Unicoi, Tennessee, Regarding The Possible Building Of A Uranium Enrichment Plant., Shannon Kathleen Sellards

Electronic Theses and Dissertations

A prolonged siting controversy for a uranium enrichment facility has occurred in the Town of Unicoi, Tennessee. One hundred-seventy residents of Unicoi were interviewed using a questionnaire regarding the building of a proposed uranium enrichment facility for Unicoi. The questionnaire sought to determine relationships between residents’ risk perceptions and 18 variables. When the Fisher’s exact procedure was applied at α< 0.05, the results indicated several associations. Odds ratio measured the strength of association. Results are reported as crude measures of association. Risk perceptions were influenced by the choice of possible locations for the facility [p=0.0003; OR=32.6]. Residents' risk perceptions were associated with a history of working with nuclear materials [p=0.0476; OR=3.2]. Finally, risk perceptions were associated with residents' beliefs that the nuclear facility would affect their health [p=0.0001; OR=18.8]. These results are discussed in light of risk perception and communication theories.

Inducing Knowledge By Enduring Experience: The Function Of A Postmodern Pragmatic Aesthetic In Linda Montano's "Living Art"., Alisa Anne Brandenburg

Electronic Theses and Dissertations

In Has Modernism Failed? (1984), Suzi Gablik calls for a postmodern art that has preserved the values that modernism lost over the 20th century, stating that only through "direct knowing" may we be shocked out of art-for-art's-sake. In Art As Experience (1934), John Dewey similarly proposes that "thinking is a kind of doing," recommending an instrumental art in one's drive to better him/herself.

Contemporary artist Linda Montano challenges the status quo by exploring the nature of art beyond its material value. In her Living Art performances (1970-1986), Montano creates a postmodern pragmatic aesthetic that is conducive to the unique …

Childhood Experiences Of Appalachian Women Who Have Experienced Intimate Partner Violence During Adulthood., Amy L. Reeves

Electronic Theses and Dissertations

Recent national surveys in the United States estimate one in five females will experience abuse by an intimate partner during her lifetime. Previous quantitative research linked childhood victimization to repeated victimization in adult relationships. This qualitative study explored the lived experiences of childhood in eight female victims of intimate partner violence who were born and reared in southern Appalachia. Interviews were analyzed using a descriptive-interpretative phenomenological method, as described by Van Manen. The three essential themes from childhood were identified as: living 'as if' an orphan; surviving in chaos; and, manifesting a devalued self. These themes were congruent with findings …

Building A Model Of Campus Diversity: An Organization-As-The-Individual Based Approach, Ian Spencer Ray

Electronic Theses and Dissertations

Diversity is a vital concept when engaging with modern social science research and among higher education practitioners. Despite the pervasive discourse surrounding diversity, equity, and inclusion (DEI) initiatives, quantification of diversity remains a challenge across the social sciences. This study proposes a new conceptual framework, applies the framework to the quantification of diversity and measurement of its impacts. Further, this study demonstrates the robustness of the new conceptual framework alongside the positive impacts of diversity on institutional outcomes.

The Organization-As-The-Individual (OATI) framework arises from an integration of the social and biological sciences. The framework argues that humans, as biological organisms, …

Early Speech And Language Development: A Comparison Of Typically Developing Children To Children With Cleft Palate., Holly Jannice Mcgahey

Electronic Theses and Dissertations

Previous research has shown that parent implemented intervention is effective in increasing the speech and language development of children with cleft lip and palate. To further determine the efficacy of this intervention, this study compared the speech and language development of children with CLP, who received parent implemented intervention, with a group of younger, typically developing children, matched for vocabulary size. This study also evaluated the language differences between the mothers of both groups of children. Speech and language assessments were administered to the typically developing children and their mothers at two times to mirror the time of assessment for …

Communication Cues To Action Prompting Central Appalachian Women To Have A Mammogram., Kathryn Bond Mcneill

Electronic Theses and Dissertations

Today, mammography screening is the best method of detection for breast cancer, yet many women have never been screened and underprivileged, minority and rural women have lower screening rates then other populations. The purpose of this study, through individual interviews(N=88), was to understand the cues that women perceive to have received spurring them to participate in mammogram screening. The Health Belief Model guided this research. Media influence, Health Care Practitioner recommendation, social networks, and symptoms were the cues to action explored in this research prompting compliance to mammography screening. All four of these cues were found to influence women in …

Perceived Barriers To The Implementation Of Web Enhancement Of Courses By Full-Time Tennessee Board Of Regents Faculty., Thomas Barron Wallace Iii

Electronic Theses and Dissertations

The purpose of this study was to examine faculty reluctance to providing students with access to course resources via the Internet. The study explored known barriers to the use of technology and the Internet within educational settings and provided opportunity for new barriers to be presented. Personal and professional demographic factors were collected to determine if certain characteristics were identifiable as predictors to web enhancement.

An online survey was designed to collect data to address research questions in the study. The survey consisted of 48 questions, including areas for comments and remarks from faculty members. One thousand two (1002) faculty, …

Characterization Of A Catechol-Type Siderophore And The Detection Of A Possible Outer Membrane Receptor Protein From Rhizobium Leguminosarum Strain Iari 312., Brianne Lee Clark

Electronic Theses and Dissertations

Many gram-negative bacteria produce and secrete siderophores under iron-deficient conditions. Siderophores are low molecular weight compounds (600-1500 Daltons), which chelate ferric iron with an extremely high affinity, and the complex is actively transported across the outer and inner membranes of gram-negative bacteria. There are two main classes of siderophores: catechol and hydroxamate. Catechol-type siderophores chelate ferric iron via hydroxyl groups, and hydroxamate-type siderophores chelate ferric iron via a carbonyl group with an adjacent nitrogen. Rhizobia fix atmospheric nitrogen symbiotically in leguminous plants using the iron-containing enzyme nitrogenase. To satisfy their iron requirements, many rhizobia are known to produce siderophores. Rhizobium …

Nongenomic Effects Of Estrogens On Epithelial Chloride Secretion., Sabyasachi Moulik

Electronic Theses and Dissertations

The human colonic cell line T84, a model for studying epithelial chloride secretion and cystic fibrosis chloride channel (CFTR) function, was used to investigate the regulatory role of estrogens in transepithelial ion transport. Estrogens and other steroid hormones do not stimulate chloride secretion by themselves. However, 17 β-estradiol (17β-E2) rapidly (within seconds to minutes) potentiates carbachol- and thapsigargin-stimulated chloride secretion measured as short circuit current in voltage-clamped T84 monolayer cultures. The cholinergic agonist carbachol and the SR Ca²⁺ ATPase inhibitor thapsigargin stimulate chloride secretion by elevating intracellular calcium. 17α-estradiol, a stereoisomer that does not activate nuclear estrogen receptors, is …

Family Literacy—Predictors Of Program Participation And Goal Attainment In Kentucky., Zelma Renae Stewart Harrison

Electronic Theses and Dissertations

The purpose of this study was to investigate how various components of family literacy programs such as operational characteristics (enrollment procedure, hours of operation, time of class, curriculum selection, type of instruction, and age of child served) and staff characteristics (gender, full or part time status, and educational attainment level) influence the recruitment, and goal attainment of adults in family literacy programs. This research investigated factors that could help educational administrators improve family literacy programs through increased enrollment, and improvement of goal attainment of participants. This exploratory, quantitative study utilized 2002-03 data submitted by family literacy programs in Kentucky. Two …

Beguiling Beginnings And Dialectical Salvaging : The Presidential Inaugural Speech And African-American Leaders' Speeches., Jacqueline Elaine Brown

Electronic Theses and Dissertations

This dissertation explores how presidential inaugural speeches reflect the overarching mindset of the government, and how, in the postmodern era, this mindset manifests the same sort of African American erasure that has existed since Middle Passage. In addition, I explore the rhetorical engagement black leaders use to respond to, prevent, or to circumvent this erasure. This dissertation examines presidential inaugural speeches, during the Civil Rights and Black Power eras, from the Kennedy administration to President Lyndon Baines Johnson, to prove that, most times, this type of speech is little more than epideictic formality in regards to black interests, and, perhaps, …

The Relationship Between Political Cynicism And Right-Wing Authoritarianism : A Study Of The Louisville-Jefferson County Merger., Jeremy Reed Porter 1978-

Electronic Theses and Dissertations

The government's ability to gain and hold a high proportion of citizens' trust is essential to the functioning of an efficient and successful administration at the federal, state, and local levels. This thesis is a quantitative study aimed at finding possible explanations for differences in levels of political cynicism. One possible explanation, right-wing authoritarianism (RWA), is the subject of this study. Furthermore, the study aims to link the variables to the 2000 Louisville-Jefferson County merger. The data for this study were collected from 802 respondents residing in the Metro Louisville area using a random technique via the 2004 Louisville Metro …

The Roles Of Pancreatic Beta Cell Antioxidants In Islet Transplantation And Type 1 Diabetes., Xiaoyan Li

Electronic Theses and Dissertations

Pancreatic beta cells are extremely vulnerable to destruction by Reactive Oxygen Species (ROS). In type 1 diabetes and islet transplantation ROS are thought to be involved in the loss of beta cells. To test the role of antioxidant in islet transplantation. In our lab we have determined that transgenic overexpression of the antioxidant protein metallothionein (MT) in pancreatic beta cells provides broad resistance to oxidative stress by scavenging most kinds of ROS. A direct test of hypoxia/reperfusion sensitivity was shown that MT markedly reduced ROS production and improved islet cell survival. Furthermore, in both syngeneic transplantation and allotransplantation, MT islets …

The Jugular Vein Of Empire : The Imperial Attachment To The Suez Canal From 1875 To 1956., Matthew Church

Electronic Theses and Dissertations

This thesis is examines the imperial attachment to the Suez Canal from 1875 to 1956. It begins with the canal share purchase by the Disraeli Government and ends with the Suez Crisis. Traditional scholarship views relations between the Britain and the Suez Canal as disjointed and highlighted by sporadic significant events. This thesis aims to evaluate the history of these relations. The thesis is divided into five chapters with the imperial attachment to the canal is divided into three chronological periods. In each phase of attachment there is at least one distinct policy shift and re-evaluation of the importance of …

3d Animation: Creating An Experiential Environment., Dorai Raj Arjunan

Electronic Theses and Dissertations

This thesis is a supporting paper for three of my 3D animations created and presented for a Master of Fine Arts graduate exhibition. It discusses how the two realms of graphic design and 3D animation helped me to develop my heuristic techniques of creating animations. Using the three animations as examples, I make an attempt to explain how linear and figurative images influence each other in the creative process of creature/character development. I also discuss the various influences and cumulative explorations behind the imageries of animation. A brief discussion about Asian Indian aesthetic concepts and the general methodology of creating …

Frequency Of Rad Diagnosis And Attachment Disorder In Community Mental Health Agency Clients., Paula S. Morgan 1958-

Electronic Theses and Dissertations

Attachment Disorder (AD) in children has been characterized by particularly alarming behaviors, yet the identification of this disorder for clinicians in community mental health agencies is problematic. The only available diagnosis that addresses attachment problems is the DSM-IV diagnosis of Reactive Attachment Disorder (RAD), a diagnosis that does not include the more severely disturbed behaviors that have been associated with attachment-disordered children. This dissertation examined the frequency with which CMHA child clients were diagnosed with RAD, as well as the actual occurrence of attachment problems and Attachment Disorder as measured by the Randolph Attachment Disorder Questionnaire (RADA). The Personality Inventory …