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The Role And Molecular Mechanisms Of Wss1 In Preserving Genomic Stability, Daniel Kwesi Sam
The Role And Molecular Mechanisms Of Wss1 In Preserving Genomic Stability, Daniel Kwesi Sam
Electronic Theses and Dissertations
Cells are constantly under threat from both exogenous and endogenous sources of DNA damage. Eukaryotic organisms, however, possess conserved mechanisms that accurately and faithfully respond to DNA damage. The inability to effectively remove DNA lesions can lead to an accumulation of mutations which can compromise cellular viability. The DNA damage response is conserved from bacteria to eukaryotic organisms and have been well characterized, however, how covalently crosslinked proteins are removed from DNA remains enigmatic This thesis provides genetic and biochemical evidence implicating Wss1, a yeast metalloprotease in genome maintenance. We have identified SUMOylation to be an important signal that mediates …
Volcanic Impact On Stratospheric Chlorine Chemistry And Perchlorate Formation: Evidence From Ice Cores, Joshua Andrew Kennedy
Volcanic Impact On Stratospheric Chlorine Chemistry And Perchlorate Formation: Evidence From Ice Cores, Joshua Andrew Kennedy
Electronic Theses and Dissertations
Perchlorate, suspected to be chemically formed in both the troposphere and stratosphere, has been recently measured in Arctic snow and ice cores. These comprise both discontinuous snow and ice cores from the Canadian Arctic and a continuous record of perchlorate was compiled from an analysis of Greenland ice cores. While the background perchlorate concentration typically is very low, a few spikes in concentration coinciding with deposition of volcanic sulfate were observed in the Greenland record, suggesting that perchlorate levels in the atmosphere may be impacted by volcanic eruptions. As of yet, no work has been done to investigate the connection …
Interaction Of Streptomyces Closely Associated With Bacillus In Nitrogen-Limiting Conditions, Aminya Baniya
Interaction Of Streptomyces Closely Associated With Bacillus In Nitrogen-Limiting Conditions, Aminya Baniya
Electronic Theses and Dissertations
Biological Nitrogen Fixation is the process of conversion of atmospheric dinitrogen into ammonia which is performed by symbiotic as well as free-living diazotrophic bacteria. A previous project to isolate free-living nitrogen fixing bacteria from a natural prairie yielded Streptomyces which grew on nitrogen free medium. Genome sequencing of three isolates revealed the presence of a second 16S rRNA gene sequence aligning with Bacillus, however repeated streaking only yielded Streptomyces colonies. The aim of this research was to characterize the interaction of three Streptomyces isolates that grow under nitrogen-free conditions, and appeared to be associated with Bacillus. The separation of Bacillus …
Biodegradable Biomaterials As Suitable Alternatives To Water Treatment Technologies, Most Farzana Yesmin
Biodegradable Biomaterials As Suitable Alternatives To Water Treatment Technologies, Most Farzana Yesmin
Electronic Theses and Dissertations
Nitrates and phosphates are essential nutrients for plants growth. Their excess presence in water, however, could cause eutrophication affecting the water quality and altering the aquatic ecosystem. Algal blooms and the presence of toxins such as microcystin in freshwater bodies are hazardous to humans, animals, and wildlife. Conventional technologies such as ion exchange, distillation, reverse osmosis, and bioreactors aid immensely to remove nutrients; however, they are expensive. Furthermore, building new nitrate and phosphate treatment units, maintenance and post-disposal are costly too. In this regard, there exists an unmet need for alternative processes that are renewable and cost-effective to treat contaminated …
Development Of Methods To Improve Lactose Recovery From Permeate And Drying Characteristics Of Greek Acid Whey, Venkateswarlu Sunkesula
Development Of Methods To Improve Lactose Recovery From Permeate And Drying Characteristics Of Greek Acid Whey, Venkateswarlu Sunkesula
Electronic Theses and Dissertations
Growth of higher protein dairy products and ingredients such as cheese, Greek yogurt, whey protein concentrates, and milk protein concentrates is limited by the amount of co-product generated and the ability to process them into value added ingredients. Finding value for all the co-products will help dairy industry to meet the growing demand for dairy protein products and ingredients. Commercial scale evaluation of new technologies for improving lactose crystallization and drying ability of Greek yogurt acid whey will help dairy industry to better utilize different dairy co-products. Commercially, lactose is manufactured from concentrated permeate by cooling and separating α-lactose monohydrate …
Determining The Effects Of Plant Extracts And Saltro Nematicide On Hatching, Mortality And Reproduction Of The Soybean Cyst Nematode (Heterodera Glycines), Bishnu Prasad Dhital
Determining The Effects Of Plant Extracts And Saltro Nematicide On Hatching, Mortality And Reproduction Of The Soybean Cyst Nematode (Heterodera Glycines), Bishnu Prasad Dhital
Electronic Theses and Dissertations
Soybean cyst nematode (SCN) is a microscopic worm that infects and feeds from the soybean roots, hence robbing the plant of nutrients, and entry wounds may facilitate other pathogens to infect the roots. Current SCN management practices include cultural, chemical, and biological methods. Cultural practices such as, crop rotation with non-host and different tillage practices are not efficient due to the persistent nature of SCN in the soil. Chemical nematicides, though effective, can be expensive, and some are not environmentally sound. SCN resistant soybean varieties are mainly derived from one source, PI 88788 and SCN population have already evolved to …
Crop Rotations, Tillage And Cover Crops Influences On Soil Health, Greenhouse Gas Emissions And Farm Profitability, Jasdeep Singh
Crop Rotations, Tillage And Cover Crops Influences On Soil Health, Greenhouse Gas Emissions And Farm Profitability, Jasdeep Singh
Electronic Theses and Dissertations
This study assessed the effects of three levels of crop rotation: [2-yr; corn (Zea mays L.)- soybean (Glycine max L.), 3-yr; corn-soybean-oat (Avena sativa L.) or 4-yr; cornsoybean- oat-winter wheat (Triticum aestivum L.)], two tillage [conventional-till (CT) and no-till (NT)], and two winter cover cropping systems [cover crop (CC) or fallow control (NC)] on soil biochemical and physical properties, greenhouse gas emissions (GHG), microbial community composition, crop yield and farm profitability under silty clay loam soil of south eastern South Dakota. Experimental design was a randomized complete block design in a split-split plot treatment arrangement with four replications. Rotations, tillage …
Exploring Heterogeneous Phenotypes In Response To Stress, Heather S. Deter
Exploring Heterogeneous Phenotypes In Response To Stress, Heather S. Deter
Electronic Theses and Dissertations
This work combines traditional microbiology with bioinformatic and synthetic biology approaches to study antibiotic tolerance. Antibiotic tolerance is a widespread phenomenon that facilitates antibiotic resistance and decreases the effectiveness of antibiotic treatment. Tolerance is distinct from antibiotic resistance, because tolerance is short term survival and typically results from phenotypic variations rather than genetic variation. The molecular mechanisms underlying tolerance are varied and debated in the literature. I have explored two intracellular processes related to tolerance, toxin-antitoxin (TA) systems (Chapter 2) and proteases (Chapter 4). Specifically, I focus on the ratio of antitoxin-to-toxin in type II TA systems, because type II …
The Impact Of Subsurface Physical Characteristics On The Effectiveness Of Subsurface Drainage In The Northern Great Plains, Mehmet Emin Budak
The Impact Of Subsurface Physical Characteristics On The Effectiveness Of Subsurface Drainage In The Northern Great Plains, Mehmet Emin Budak
Electronic Theses and Dissertations
World-wide, salinity and sodicity problems are increasing in coastal, irrigated and dryland agricultural systems. Traditional reclamation techniques for saline sodic soils include improving soil drainage by installing subsurface drainage, leaching with high quality water, and applying a source of calcium. However, due to differences in the soil parent material these traditional approach treatments were ineffective in removing sodium and other salts out of the soil profile of South Dakota. Understanding how the surface and subsurface soil characteristics and management interact to affect the sustainability of these systems is the first step in remediation. Each region and soil have a slightly …
"It's None Of Your Damn Business" : Communication Among Newlyweds Regarding Family Expansion Preferences, Kiersten Koehler
"It's None Of Your Damn Business" : Communication Among Newlyweds Regarding Family Expansion Preferences, Kiersten Koehler
Electronic Theses and Dissertations
As newlyweds begin their new adventure as a married couple, the discussion of family expansion is inevitable. This thesis seeks to understand how newlyweds discuss their plans for family expansion among each other and to others by applying the theory of communication privacy management. Ten newlywed couples were interviewed regarding their experiences navigating the topic of family expansion and reproduction. Specifically, the couples were asked about preferences for privacy and control, and what expectations the couples have when disclosing information about their preferences for family expansion. The data demonstrated that communication privacy management may not look the same among each …
Rednecks And Hillbillies: A Thematic Analysis Of The Construction Of Pride And High Self-Esteem Exhibited By Southern Characters, Casey R. White
Rednecks And Hillbillies: A Thematic Analysis Of The Construction Of Pride And High Self-Esteem Exhibited By Southern Characters, Casey R. White
Electronic Theses and Dissertations
The purpose of this study is to examine television shows portraying redneck and hillbilly culture from 2010 to 2017 to examine the limitations of focusing on traditional stereotypes about southern culture when understanding the complexities of redneck and hillbilly society. According to a literature review, redneck and hillbilly characters have historically been associated with poverty, have been used as comedic symbols to portray southerners in the lower classes as jokes, and the terms have been used as insults. A thematic analysis was conducted analyzing redneck/hillbilly themed television shows from the years 2010 through 2017 to examine them for the presence …
Teachers' Perceptions Of Experiential Learning In The 3-Circle Agricultural Education Model, Jon C. Schreurs
Teachers' Perceptions Of Experiential Learning In The 3-Circle Agricultural Education Model, Jon C. Schreurs
Electronic Theses and Dissertations
The purpose of this paper is to describe teachers’ perceptions of experiential learning and its effects on student learning, understanding, and engagement through agriculture education's three-circle model. This paper explores teachers’ perceptions of the value and importance of experiential learning in agriculture education instruction, FFA, and supervised agricultural experience. Further, I explore experiential learning, constructivism, and hands-on learning as the foundational instructional practices used in agricultural education. Agricultural education teachers from southwest Minnesota were surveyed to determine how they are using experiential learning in their agriculture education programs, including classroom instruction, FFA, and supervised agricultural experiences.
Early Adolescent Social Isolation, Hope, And Well-Being During A Pandemic, Alicen Hauck
Early Adolescent Social Isolation, Hope, And Well-Being During A Pandemic, Alicen Hauck
Electronic Theses and Dissertations
Social isolation is often divided into two subcategories of objective and subjective. The COVID-19 pandemic has led to an increase in objective social isolation in the form of social distancing and fewer social events. Research delineating the relationship between social isolation and adolescent well-being utilize measures of subjective social isolation. Whereas, measures of objective social isolation are more commonly used with geriatric populations. Therefore, there is a lack of information specific to the impact of objective social isolation on adolescent well-being, particularly during a pandemic. The effects of social isolation due to COVID-19 will not be short lived. Deciphering the …
Technology-Based Culturally Relevant Chemistry Education (Crce), Mercy Adoma Fosu
Technology-Based Culturally Relevant Chemistry Education (Crce), Mercy Adoma Fosu
Electronic Theses and Dissertations
The relevance of chemistry concepts and principles remains a challenge for most students enrolled in college chemistry courses. Part of this problem is the disconnect with what students experience in their every-day lives, and how the material, specifically chemistry content is presented via textbooks, lectures, and laboratory teaching. Moreover, the teaching of general chemistry has traditionally focused on traditional, instructor-centric expository delivery of course materials, that treats students as empty vessels devoid of prior knowledge. Students prior knowledge and experiences are crucial in shaping their understanding of scientific concepts and principles. Even students with relatively low expectations for success in …
Evaluation Of Fall-Seeded Cover Crops For Grassland Nesting Waterfowl In Eastern South Dakota, Charles W. Gallman Iii
Evaluation Of Fall-Seeded Cover Crops For Grassland Nesting Waterfowl In Eastern South Dakota, Charles W. Gallman Iii
Electronic Theses and Dissertations
The Prairie Pothole Region is the primary breeding ground for many species of North American waterfowl. This landscape was historically dominated by mixed and tallgrass prairies interspersed with wetlands, but >70% of native grassland area has been lost due to widespread conversion to croplands, which may threaten waterfowl production. Cover cropping is a re-emerging farming technique that may provide suitable nesting cover for grassland nesting waterfowl on active farmlands. My research objectives were to evaluate the utility of fall-seeded cover crops to breeding waterfowl compared to perennial cover, determine if cover crops in rotation with row crops can successfully support …
Monitoring Methods And Performance Assessment For Implementation Of Low Impact Development Practices In South Dakota, Farhana Akhter
Monitoring Methods And Performance Assessment For Implementation Of Low Impact Development Practices In South Dakota, Farhana Akhter
Electronic Theses and Dissertations
Urbanization increases impervious surface area, which changes the hydrology of a watershed. Impervious surfaces prevent the infiltration of stormwater water into the ground surface which results in a higher volume of stormwater runoff and higher peak flow. Low Impact Development (LID) practices help to restore pre-development hydrology by increasing infiltration, evaporation, and transpiration. While the hydrologic performance of LID practices has been studied extensively and continues to be an area of active research, there has been very limited monitoring or demonstration of LID practices in South Dakota. In addition, municipalities in South Dakota and elsewhere that implement new LID practices …
Developing Microbial Based Process To Produce High Value Natural Antimicrobial (Glyceollin) In Soybeans And Pullulan From Sucrose, Andrea Zavadil
Developing Microbial Based Process To Produce High Value Natural Antimicrobial (Glyceollin) In Soybeans And Pullulan From Sucrose, Andrea Zavadil
Electronic Theses and Dissertations
Glyceollin is a secondary metabolite produced under stress conditions by soybean to act as a plant defender to pathogen attack. The antimicrobial nature of glyceollin makes it a promising natural alternative to antibiotics if incorporated into livestock feed. However, production of glyceollin is highly variable, dependent on soybean variety, and fungal elicitor used as inoculum. Our study compares two fungal spore elicitors, Trichoderma reesei and Aspergillus sojae on soybeans of diverse maturity level, and fungal susceptibility to determine highest glyceollin titer. Spore inoculation with T. reesei elicited highest glyceollin titers of 2.42±0.20 mg/g at 120 h, while A. sojae elicited …
Reducing Gaps In Knowledge About Electroconvulsive Therapy Among Mental Health Nurses, Janice L. Edmonson
Reducing Gaps In Knowledge About Electroconvulsive Therapy Among Mental Health Nurses, Janice L. Edmonson
Electronic Theses and Dissertations
This DNP study was conducted to identify knowledge gaps about electroconvulsive therapy (ECT) among mental health nurses and to determine the effectiveness of an educational intervention on the knowledge and attitude of mental health nurses about the use of ECT at a psychiatric hospital. In this study, the researcher sought to build upon an existing knowledge base using principles of adult learning theory to enhance the therapeutic relationship of the nursing staff when caring for ECT patients. A sample of three-quarters (N = 119) of the mental health nursing staff employed by the psychiatric hospital consented to participate in …
A Black Feminist Quare Interrogation Of Stud Misogyny In Black Queer Web Series, Taisha Mcmickens
A Black Feminist Quare Interrogation Of Stud Misogyny In Black Queer Web Series, Taisha Mcmickens
Electronic Theses and Dissertations
The purpose of this Black feminist-quare study is to analyze the relationship between misogyny and queer masculinity performed by “studs” in Black queer web series located on YouTube.com: Women of Atlanta TV, New York Girls TV, Choiices The Series, and The Best Friend. Studs are masculine-identified Black lesbians. Stud misogyny is tethered to histories of the patriarchal gaze on Black women’s bodies. This gaze exposes stud and femme queers to layers of violence challenging us to rethink masculinities outside of the colonial imagination. I employ a Black Feminist Quare theoretical framework to attend to stud’s embodied experiences, challenge …
Do These Violent Delights Have Violent Ends? Social Norms, Alcohol, & Sexual Cyberbullying, Anandi C. Ehman
Do These Violent Delights Have Violent Ends? Social Norms, Alcohol, & Sexual Cyberbullying, Anandi C. Ehman
Electronic Theses and Dissertations
Sexually aggressive behavior is well-documented among college students. However, little is known about the role technology may play in facilitating this behavior. Given that social norms have been established as a useful framework for understanding problematic and risky behavior in college students, the current authors sought to determine whether this theory might also provide insight into the use of technology to facilitate sexually aggressive behavior. Thus, this work sought to determine whether sexually aggressive behavior which occurs through the use of technology and social media, henceforth known as sexual cyberbullying, mediated the relationship between perceived social norms of sexually aggressive …
Sec Athletics And Engagement Of Fans, John Harrison Rowland
Sec Athletics And Engagement Of Fans, John Harrison Rowland
Electronic Theses and Dissertations
The Southeastern Conference (SEC) is one of the premiere affiliate conferences of the National Collegiate Athletic Association (NCAA). Sports of all kinds have, for some time, engaged fans in multiple ways with the advent of social media enabling new forms of engagement. One of the most popular social media platforms for fan engagement is Twitter. This study is one of the first to explore how SEC teams use Twitter to interact with fans. After downloading Twitter data from all SEC baseball and football accounts, engagement was established. Engagement was defined as retweets plus favorites. With engagement defined and used as …
Lidocaine Mucoadhesive Film Fabrication Using Fused Deposition Modeling 3d Printing, Mohammed Yahya Alyahya
Lidocaine Mucoadhesive Film Fabrication Using Fused Deposition Modeling 3d Printing, Mohammed Yahya Alyahya
Electronic Theses and Dissertations
Hot-melt extrusion and 3D printing are among the recent means of processing techniques adopted in the pharmaceutical industry. These technologies are used as a method of preparing solid dispersions develop complex shapes that are difficult to be obtained using other means of production. In the current research work, many formulations loaded with lidocaine HCl were prepared and extruded using 11mm twin-screw extruder. The extrudate filaments were then fed into a fused deposition modelling (FDM) printer to fabricate mucoadhesive films meant to buccal applications to manage local pain within the oral cavity that requires the intervention with local anesthetics. The printed …
Approaches To Alleviate The Vigabatrin Induced Retinal Toxicity, Anitha Police
Approaches To Alleviate The Vigabatrin Induced Retinal Toxicity, Anitha Police
Electronic Theses and Dissertations
Safety of therapeutics is one of the primary concerns during the treatment of any ailments. Most of the toxic effects of drugs occurs due to accumulation of drug in organs, which is dependent on the dosage of the drug. The ideal therapy should minimize the risk of causing adverse events at the same time elicit intended therapeutic effects. Vigabatrin (VGB) is an antiepileptic drug used primarily in the treatment of infantile spasms and it acts by inhibiting gamma amino butyric acid (GABA) transaminase thereby increasing inhibitory neurotransmitter GABA levels. At therapeutic doses, VGB accumulates in retina leading to peripheral visual …
Service-Learning, Comcore, And The Secondary English Classroom: A Mixed Methods Study, Kimual Snow
Service-Learning, Comcore, And The Secondary English Classroom: A Mixed Methods Study, Kimual Snow
Electronic Theses and Dissertations
ABSTRACT The prevalence of high-stakes, standardized testing, along with the emergence of the ComCore State Standards has decreased teachers’ capacity to establish and implement authentic learning opportunities for students in 21st century secondary English classroom. In a progressive literacy environment, teachers must commit to real-world learning that allows students to navigate complex informational texts without sacrificing high curriculum standards. A number of qualitative studies reveal how service-learning has been used in various classrooms to address deficiencies in student motivation and engagement, which in turn, sought to increase students’ academic outcomes. However, few provide the quantitative evidence that administrators want to …
The Charger: A Case Study Of Leadership Decisions And The Oxford High School Student Newspaper, R. J. Morgan
The Charger: A Case Study Of Leadership Decisions And The Oxford High School Student Newspaper, R. J. Morgan
Electronic Theses and Dissertations
This case study uses qualitative interviews with administrators, advisers, former editors, and others, along with researcher observations and more than 50 years of archival documents to construct a comprehensive portrait of the history, successes, and challenges of the student newspaper program at Oxford High School, and how administrative decision making has affected that success and vice versa. The OHS student newspaper, called the Charger since the late 1980s, has consistently ranked at or near the top of the best high school newspapers in Mississippi and has at times been named one of the top high school newspapers in the nation. …
Development Of A Liposomal Formulation For Brain Targeting, Ziru Zhang
Development Of A Liposomal Formulation For Brain Targeting, Ziru Zhang
Electronic Theses and Dissertations
The blood-brain barrier (BBB), formed by the endothelial cells of the brain capillaries, inhibits the penetration of many therapeutic compounds into the brain. Liposomes, which have unique physicochemical characteristics, have been widely investigated for the drug delivery system across BBB. In the current study, FITC-dextran was used as a model drug that encapsulated in a liposomal formulation to investigated its ability to overcome the blood-brain barrier for targeted brain delivery of therapeutic agents. Here, non-targeted liposome (NT LPs) and RVG-modified liposome (RVG LPs) were prepared. The NT LPs and RVG LPs were about 97 and 101 nm in diameter with …
Awareness Of Peace Journalism Among Bangladeshi And Ethiopian Journalists, Sima Rani Bhowmik
Awareness Of Peace Journalism Among Bangladeshi And Ethiopian Journalists, Sima Rani Bhowmik
Electronic Theses and Dissertations
News is a vital element to keep people informed. Though the orientation of the stories is often debated over whether it is based on facts or lies. In a polarized society, it may not be the event that contributes to violence, but how the event is reported. Here is where the role of peace journalism comes in where reporters or news managers can shed light on structural and cultural causes of violence. It is in this context that this study attempted to investigate the level of awareness of peace journalism among Bangladeshi and Ethiopian journalists. To this end, the researcher …
Role Of Insulin-Like Growth Factor 1 Receptor In The Regulation Of Astrocyte Structure, Function And Cognition, Disha Prabhu
Role Of Insulin-Like Growth Factor 1 Receptor In The Regulation Of Astrocyte Structure, Function And Cognition, Disha Prabhu
Electronic Theses and Dissertations
Levels of Insulin-like growth factor I (IGF-1) are reduced in several neurological disorders, including aging and neurodegeneration. Aged animals have reduced IGF1 signaling and exhibit impairment in cognition. Supplementing rodents with IGF-1 reverses some of the cognitive deficit, suggesting that IGF-1 signaling could potentially be targeted to ameliorate cognitive decline in older adults. For this, a thorough understanding of the mechanisms by which IGF-1 regulates the function of various cell types in the brain is needed. A majority of studies have focused on the effects of IGF-1 regulating neurons. However, astrocytes that form roughly about 40% of brain mass also …
Comparing The Variability Of Natural Sand To The Variability Of Sand Containing A Simulant Land Mine, Anna Marie Mcwhirter
Comparing The Variability Of Natural Sand To The Variability Of Sand Containing A Simulant Land Mine, Anna Marie Mcwhirter
Electronic Theses and Dissertations
Understanding the relationship between excitation sources, buried target (i.e., buried hazard, land mine, acoustic article) response, and soil properties is fundamental to improve laser-ground-vibration sensing methods. This project investigates the natural soil’s behavior under acoustic stimuli and compares soil behavior with a buried target through geostatistical methods. Vibrational velocity of sand is measured with an LDV in a confined box filled with and without a buried target. Geostatistical calculations were performed on standardized data (e.g., background velocity and with-target velocity) sets to observe spatial variability. The standardized background velocity is mean 0 and variance of 1, while the addition of …
The Southern Front: Gay Liberation Activists In The U.S. South And Public History Through Audiovisual Exhibition, David Hooper Schultz
The Southern Front: Gay Liberation Activists In The U.S. South And Public History Through Audiovisual Exhibition, David Hooper Schultz
Electronic Theses and Dissertations
This project expands upon the historical work done in the master’s thesis “The Carolina Gay Association, The Southeastern Gay Conferences, and Gay Liberation in the 1970s South,” and builds on its work through an examination of public history’s impact for LGBTQ+ southerners. The audiovisual exhibit, both physically and online, investigates queer southern activism within the context of the Carolina Gay Association and its subsequent conferences, the Southeastern Gay Conferences. The public history work uncovers how activists remember their own involvement within the organization and how they were connected to national conversations surrounding gay liberation. The photography portion of the project …