Digital Commons Network^™

Optimized Microbial Recombinant Production Of Hiv-1 Anti-Envelope Antibody Fragments With Applications To Single Particle Tracking Of Virus Assembly, Merissa Michelle Bruns

Electronic Theses and Dissertations

In my findings, I have established a set series of protocols to recombinantly produce, purify and apply various fluorescent probes in vitro for the fluorescent labeling and study of the human immunodeficiency virus type 1 (HIV-1) envelope (Env) protein during HIV viral assembly. There remains insufficient knowledge about the molecular dynamics and interactions of HIV-1 Env protein with its counterpart, Gag, on the inner host cell surface during assembly of a mature virus particle. There also remains an insufficient amount of data for the understanding and clarification of the mechanism of action of a known host cell HIV-1 restriction factor, …

War Of Words: A Rhetorical Analysis Of The Military's Sexual Assault Prevention Posters, Nancy Thurman Clemens

Electronic Theses and Dissertations

Joining the expanding discourse surrounding language and its effects, specifically regarding the performance of gender in a hypermasculine environment, this dissertation offers a rhetorical analysis of the United States Department of Defense's sexual assault prevention and response training materials, particularly posters created between 2009 and 2012. This dissertation examines the context of sexual harassment and assault within the military from the late 1970s until the mid-2000s. Presenting scandals that led up to the development of the Department's Sexual Assault Prevention and Response (SAPR) program, I give a brief history of the establishment and scope of responsibility for the program in …

Criminalized Houseless Women, Jesus, And The Praxis Of Kinship: An Outsider’S Liberative Ethic, Sarah A. Neeley

Electronic Theses and Dissertations

Criminalization measures, such as Denver’s Urban Camping Ban, are an attempt to mask systemic causes of houselessness and assign blame to the personal failures of those living on the streets. Unaccompanied houseless women utilize street families to create community, survive houselessness, and fight criminalization measures. Since liberation and solidarity can become forces of oppression, this dissertation considers cultural evidence for the desire and means of liberation. Street families and the praxis of kinship form the basis of a liberative ethic that critiques systemic causes of houselessness and provides a model of relatedness that works toward social justice. The project is …

Deep Learning Methods For Fingerprint-Based Indoor And Outdoor Positioning, Fahad Alhomayani

Electronic Theses and Dissertations

Outdoor positioning systems based on the Global Navigation Satellite System have several shortcomings that have deemed their use for indoor positioning impractical. Location fingerprinting, which utilizes machine learning, has emerged as a viable method and solution for indoor positioning due to its simple concept and accurate performance. In the past, shallow learning algorithms were traditionally used in location fingerprinting. Recently, the research community started utilizing deep learning methods for fingerprinting after witnessing the great success and superiority these methods have over traditional/shallow machine learning algorithms. The contribution of this dissertation is fourfold:

First, a Convolutional Neural Network (CNN)-based method for …

Recycling Of Fiber Polymer Matrix Composites In Cementitious Materials, Edward Patton Clark

Electronic Theses and Dissertations

Recycling options for fiber polymer matrix composite waste materials are limited because they typically cannot be reused, reprocessed for down-cycling, and are generally environmentally unfriendly. The utility industry, specifically electrical distribution, has been increasingly using hybrid carbon, glass fiber, and epoxy resin composite rods for high-voltage (HV) conductor transmission lines. The high-voltage conductor core (HVCC) used in the transmission line can have an optimal in-service-life of roughly several decades, in which the material is then retired to a waste landfill. Currently, there is limited research and recycling methodology for these hybrid composite rods. In this research, powder carbon fiber, glass …

Lebanese Colonial Hang-Ups: Anti-Blackness And The Kafala System In Lebanon, Sarah Gonzalez Noveiri

Electronic Theses and Dissertations

Lebanon’s colonial legacy has not only influenced the legal and political systems which operate in the country today. As I hope to demonstrate in this dissertation, the strength and cementation of these systems can be seen as a consequence of negotiations of identity, citizenship and nation state that took place during the Ottoman Empire and French colonial rule. The Kafala is one of such systems that developed through the Ottoman Empire and against the backdrop of European colonization. Discourses of race, citizenship, gender and sexuality that were being negotiated by colonial citizens and colonial powers, served to cement the racialized …

Resentenced And Released: Re-Entry Needs Following Release From Juvenile Life Without Parole, Daphne M. Brydon

Electronic Theses and Dissertations

Over 2,100 individuals serving juvenile life without paroles (JLWOP) sentences in the U.S. became eligible for resentencing following the 2016 Montgomery v. Louisiana Supreme Court ruling. Michigan housed an estimated 370 juvenile lifers at that time, the second largest JLWOP community in the country and has since resentenced and released approximately 120 juvenile lifers. Folx released from prison encounter many barriers to successful re-entry. Barriers are often amplified for those incarcerated as adolescents. Further, services are de-prioritized for folx serving JLWOP sentences, which can be especially damaging for this community whose life experiences are marked by high rates of trauma, …

A Grounded Theory Inquiry Into The Pedagogical Socialization Of Graduate Students Within Graduate Quantitative Methods Courses, Amanda Kay Thomas

Electronic Theses and Dissertations

Quantitative methods are one of the most highly technical fields of study within social sciences graduate programs. Although classroom pedagogy is an important factor connected to student success within graduate quantitative methods courses little is known on the pedagogical socialization experiences of masters and doctoral students. The purpose of this grounded theory inquiry was to discover graduate students perspectives on their pedagogical socialization experiences and the norms, values and role expectations transmitted during the teaching and learning of quantitative methods. Narrative data was collected from in-depth interviews among a theoretical sample of 31 masters and doctoral students enrolled in introductory, …

Operationalizing A New Method For Defining And Scaling Social Innovations Using Tiny Home Communities As A Case Study, Jennifer H. Wilson

Electronic Theses and Dissertations

Social innovations are new approaches to addressing unmet need. In an increasingly globalized and interconnected world, social innovations propagate rapidly in response to the dynamic conditions of our modern world. An example of an emergent social innovation, tiny home communities are gaining traction as a more economically, socially, and environmentally favorable response to homelessness and inadequacies in the current shelter and housing system. The use of tiny homes communities (that is, intentional clusters of small-scale structures) as an innovative response to homelessness is relatively new. As such, there is limited empirical evidence on the topic. Lack of research and defined …

Elucidating The Interfacial Interactions Between Biomacromolecules And Functionalized Pamam-Based Materials For Biomedical Applications, Briana L. Simms

Electronic Theses and Dissertations

Polyamidoamine (PAMAM) is a dendrimer structure with a polypeptide backbone architecture. It has been utilized in a wide variety of applications but has found significant utility in biomedical applications. PAMAM has been studied extensively due to its globular structure that is similar to proteins found in the body. The branched architecture and overall size provide a favorable environment for host-guest interactions and allows for the loading of small molecules for therapeutic delivery. Although PAMAM has many benefits for use in the area of therapeutic delivery, a primary area of concern for this molecular architecture is the terminal amines that serve …

A Spectroscopic And Quantum Chemical Investigation Into The Noncovalent Interactions And Charge Accommodation Of Biomolecules And Their Building Blocks In Various Environments, Ashley Elizabeth Williams

Electronic Theses and Dissertations

As optical measurements have grown increasingly more precise with the rage of the technological revolution, the computer’s ability has matched in computing speed to produce accurate quantum chemical predictions of systems ranging from the atomic to multi-molecular levels. Application of spectroscopy in the investigation of the structure and function of biomolecules and their building blocks has a rich literature background, with applications among cancer research, charge transfer and energy storage, and drug design and delivery. The focus of this dissertation is thus a quantum chemical and/or spectroscopic investigation of a series of biomolecules which include the vibrational band positions of …

"Beyond The Lines": A Reassessment Of Civil War Prisons, Beth Kruse

Electronic Theses and Dissertations

“Beyond the Lines”: A Reassessment of Civil War Prisons, challenges the historical interpretations of Civil War military prisons. Specifically, it analyses the political, social, and economic conditions of these systems by not only adding omitted gender, class, and race scholarship but flushing out the power dynamics between these group and military administrations. The re-examination of primary source material by reading against the grain to find overlooked insights reveals these sources not only provide a wealth of information about omitted groups, but that they have been misinterpreted. Additionally, applying the concepts of historical memory establishes how the Lost Cause shaped not …

Therapeutic Immunoglobulin G Surface Mobility Promotes Antibody-Dependent Cellular Phagocytosis By Arp2/3 And Syk Mediated Rearrangement Of Fcγ Receptors, Seongwan Jo

Electronic Theses and Dissertations

Therapeutic antibodies are rapidly growing class of drugs for treating cancers and immunological disorders by targeting specific antigens or blocking certain pathway to inhibit the tumor growth. In cancer immunotherapy, it was found that Fcγ receptor (FcγR) in immune cells play an important role in antibody dependent cell toxicity against tumor cells. Therefore, understanding FcγR activation mechanism, which requires ligation with immunoglobulin (IgG), is crucial to enhance potency of therapeutic antibodies. One crucial immune responses triggered upon FcγR ligation with IgG is phagocytosis which indicates clearing targets by engulfment. It is known that FcγRs form microclusters which gets rearranged during …

Milk Phospholipids Extraction Using Switchable Solvents, Kaavya Rathnakumar

Electronic Theses and Dissertations

Phospholipids (PLs) found in milk are of great interest due to their health and nutritional benefits associated with their consumption. By-products generated during the manufacture of dairy products represent a source of PLs with potential for value-added opportunities. Currently, the extraction of PLs from by-products results in overall lowefficiencies, and it involves subsequent solvent separation and lipid recovery. This dissertation studied the extraction of milk PLs from by-product streams with a tertiary anime (N, N-dimethyl cyclohexylamine, CyNMe2) as a switchable hydrophilicity solvent. This type of solvents can be reversibly switched between a hydrophobic and hydrophilic form in the presence or …

A Biocontrol Pesticide Derived From Mycovirus-Infected Sclerotinia Sclerotiorum Can Induce Plant Resistance, Connor Pedersen

Electronic Theses and Dissertations

Soybean leaf-associated gemycircularvirus-1 (SlaGemV-1) is a novel mycovirus discovered through the metagenomic sequencing of soybean leaves which is capable of inducing hypovirulence in the highly pathogenic fungus Sclerotinia sclerotiorum. RNASeq analysis techniques were used to determine the transcriptional changes caused by the infection of virus in S. sclerotiorum, as well as the transcriptional changes in Glycine max caused by the colonization of hypovirulent, SlaGemV-1-infected S. sclerotiorum. RNASeq results indicate that viral infection leading to hypovirulence may attenuate expression of genes relating to cell wall synthesis, microtubule formation, and metabolism of steroids and natural antibiotics. Cytochrome P450-related genes, kinesin domain genes, …

Nitrate And Phosphate Removal From Denitrification Bioreactors Using Woodchips, Steel Chips And Agricultural Residue Media, Abdoul Aziz Kouanda

Electronic Theses and Dissertations

Nitrate and phosphate present in agricultural subsurface drainage water can impact surface water quality thus impacting the environment and human health by causing eutrophication and Methemoglobinemia in young infant. Biological denitrification and phosphate adsorption filters are treatments options that can help reduce nitrate and phosphate concentration from agricultural subsurface drainage before it can be discharged in surface water. The first objective of this dissertation was to determine the Michaelis-Menten kinetics model parameters for nitrate removal in bioreactors using the fresh, aged, and composted woodchips. The composted woodchips achieved a denitrification rate 2.4 times higher than the fresh woodchips which was …

Analysis Of Volatile Organic Compounds: Petroleum Emissions And Microbial Degradation, A K M Ahsan Ahmed

Electronic Theses and Dissertations

Volatile organic compounds (VOCs) are abundant in nature and can occur in various forms in a wide variety of sources. Petroleum is one of the major sources of VOCs. Petroleum being the most widely used energy resources of the world, often released to the environment due to emission and leakage or accidental spillage during exploration, transportation and manufacturing. Once released to the environment, petroleum poses a serious threat to the environment due to some of its toxic organic components. Therefore, the analysis of petroleum is critical to understand its effect on the environment and remediation. This dissertation is focused on …

College Students Conceptions Of Particulate Nature Of Matter And The Impact On Research Instrumentation, Bridget Klutse

Electronic Theses and Dissertations

The main objective of this research was to explore the understanding and conceptions of Particulate Nature of Matter (PNM) with Chem 115 L students as the participants. Conceptions were defined specifically as beliefs and alternative beliefs about topics. The research also assessed the impact of analytical instrumentation in the chemistry laboratory on learning chemistry concepts. Eight questions (8) with multiple choice answers were administered to 10 students at the beginning and after the Fall 2018 semester via selective/purposeful sampling. Data were collected using surveys (pre- and post-surveys) and interviews (pre- and postinterviews), then analyzed quantitatively and qualitatively. The eight questions …

Exploration Of Host Health Benefits By A Defined Consortium Of Butyrate-Producing Human Gut Bacteria In Gnotobiotic Mouse Model, Linto Antony

Electronic Theses and Dissertations

Aberrant gut microbiota composition is found to be associated with several human diseases such as inflammatory bowel diseases (IBD). Reduction in butyrate producing bacteria is one of the characteristic features of such dysbiotic bacterial community in the gut. Modulation of gut microbiota to bring the dysbiotic state back to normal healthy state is a promising therapeutic strategy to cure several diseases like recurrent Clostridium difficile infection (rCDI) and IBD where traditional therapies using pharmacological substances fails to make a difference. Restoration of butyrate producers is found to be an effective method of such gut microbiota modulation. The potential to produce …

Improved Secure And Low Computation Authentication Protocol For Wireless Body Area Network With Ecc And 2d Hash Chain, Soohyeon Choi

Electronic Theses and Dissertations

Since technologies have been developing rapidly, Wireless Body Area Network (WBAN) has emerged as a promising technique for healthcare systems. People can monitor patients’ body condition and collect data remotely and continuously by using WBAN with small and compact wearable sensors. These sensors can be located in, on, and around the patient’s body and measure the patient’s health condition. Afterwards sensor nodes send the data via short-range wireless communication techniques to an intermediate node. The WBANs deal with critical health data, therefore, secure communication within the WBAN is important. There are important criteria in designing a security protocol for a …

Electrical Sensing In Non-Excitable Cells To Promote Galvanotaxis And Tissue Survival, Anyesha Sarkar

Electronic Theses and Dissertations

Weak DC fields have been shown to induce polarity, cell migration and cell proliferation in 2D cultures in vitro. To understand the mechanism by which non-excitable cells sense such weak EFs, we have investigated the mechanism of cathode-directed water flow (electro-osmosis) in the boundary layer of cells by reducing it with neutral, viscous polymers. Our results indicate that low molecular weight polymers decrease cathodal migration and promote anodal migration in a concentration dependent manner. High molecular weight polymers do not affect directionality and can be explained using porosity and hydraulic permeability between the polymers. These results provide the first evidence …

The Role Of The Auxin Biosynthesis And Signaling In Soybean Root Nodule Development, Mucahid Bozkus

Electronic Theses and Dissertations

Nitrogen(N) is one of the most important plant nutrients for plant growth and yield, however, its abundance in the soil is not sufficient for profitable crop production. The use of chemical fertilizers helps address soil N deficiency in agriculture. However, due to the environmental pollution resulting from excessive use of fertilizers, alternative forms of N for agriculture are a necessity. Leguminous plants such as soybean (Glycine max) form a symbiotic association with N-fixing rhizobia to meet their N demands. Legume-rhizobia symbiosis results in the formation of unique structures called nodules where rhizobia convert atmospheric nitrogen into plant usable forms, thus …

Rational Design Of Advanced Functional Materials For Electrochemical Devices, Shun Lu

Electronic Theses and Dissertations

In recent years, there has been a fast-growing trend in developing urea (CO(NH2)2) as a substitute H2 carrier in energy conversion due to its high energy density, nontoxicity, stability, and nonflammability. Urea, a byproduct in the metabolism of proteins and a frequent contaminant in wastewater, is an abundant compound that has demonstrated favorable characteristics as a hydrogen-rich fuel source with 6.7 wt % gravimetric hydrogen content. Also, there is 2-2.5 wt % urea from mammal urine; therefore, 0.5 million ton of additional fuels will be produced per year just from human urine (240 million ton each year). Electrochemical oxidation has …

An Evaluation Of The Bighorn Sheep Population In Badlands National Park, Austin J. Wieseler

Electronic Theses and Dissertations

Within the last century, bighorn sheep (Ovis canadensis) in the badlands ecosystem of western South Dakota have been subjected to complete extirpation, reintroduction, disease die-offs, genetic bottlenecking, and population augmentation. Subsequently, the population in Badlands National Park (BNP) appears to have recovered, but it was unknown to what degree past events had influenced the population. From 2017-2019, we conducted research on 5 subherds within 2 management units in BNP to 1) survey for the presence of respiratory pathogens and estimate the prevalence of other potentially infectious diseases; 2) assess adult and lamb survival and cause-specific mortality; 3) estimate population size …

Improving Nutritional Properties And Extractability Of Pea Proteins For Human Consumption Via Fungal Bioprocessing, Camille Massmann

Electronic Theses and Dissertations

Yellow peas are a rich source of protein and phytochemicals. Peas are compatible with many diets restricted by sensitivities, allergies, or personal choice. As a result, peas are gaining popularity in food markets, despite the challenges they present. The biggest challenges for pea proteins in food markets are high content of fiber, starch, and saponins. These components cause processing challenges and create undesirable textures and bitter flavors. Pea proteins are typically extracted with alkaline extraction or air classification methods which result in 80% and 50% protein products respectively. With low protein purity, these products are not competitive with other, more …

Encroachment: College Athletes’ Experiences With Identity Development As Affected By Media Representations Of Social Justice Demonstrations, Madison Remi Vanwalleghen

Electronic Theses and Dissertations

This study was conducted to understand how communication and identity are experienced by Black university athletes in asking about their experiences and how they gauge mainstream media portrayals of Black NFL athletes making verbal or physical statements about or in protest of social injustice, racism, and inequality in America. I interviewed five university athletes identifying as Black/African American or Mixed- Race using media representations of Black NFL athletes’ protests since the NFL has dominated mainstream media headlines regarding recent athletes’ protests, especially in connection to the Black Lives Matter social movement. I also used several theories to interpret the data …

Prebriefing In Simulation, Brittany A. Brennan

Electronic Theses and Dissertations

No abstract provided.

The Effects Of Hip Rotation On Hip Abductor Muscle Activation During Lateral Band Walk Exercise, Gina M. Fritz

Electronic Theses and Dissertations

BACKGROUND: Lateral band walks (LBW) have been used to strengthen hip abductors and decrease risk of lower extremity injuries. Several methods have been used to complete this exercise to determine which has the best outcome. PURPOSE: The purpose of this study is to compare muscle activation of the gluteus medius (Gmed), gluteus maximus (Gmax), and tensor fasciae latae (TFL) during straight leg LBW exercises performed using three different hip positions: 30-degree internal rotation, 30-degree external rotation, and a neutral hip position. METHODS: Thirty-seven recreationally active participants (10 male, 27 female; age=21.4±2.2 yr; mass=71.4±13kg; height=1.7±0.1 m) completed this study. Surface EMG …

Catholic Elementary School Principals, The Transfer Of Marketing Knowledge, And The Diffusion Of School Marketing, Lisa A. Geosits

Electronic Theses and Dissertations

Despite elementary Catholic schools engaging in school marketing for some years, the level of knowledge of the school leaders and the challenges related to school marketing and the implementation of a marketing framework is not known. The researcher focused this qualitative case study on identifying the key relationship marketing performance indicators and the diffusion of school marketing that contribute to a sustainable organization after the implementation of marketing and culture change. The research questions explored the main knowledge, skills, and abilities related to marketing and leadership that can be used to aid the elementary Catholic school leaders to assist in …

Cross-Layer Optimization On Wireless Visual Sensor Networks For 3d Indoor Monitoring: A Top-Down Approach, Zhonghui Wang

Electronic Theses and Dissertations

As one of the latest advances in wireless sensor network (WSN), wireless visual sensor network (WVSN) bears a number of distinct features compared to its predecessor, mainly due to its directional visual coverage on the sensing field. This also renders previous solutions proposed for WSN may not work well for WVSN. For example, k-coverage has been introduced to WSNs for fault tolerance and high monitoring quality by omni-directional sensors but becomes incapable to cope with the directional coverage of sensors in WVSNs. Moreover, compared to scalar data in WSNs, visual data are much larger, difficult to aggregate and usually streamed …