Digital Commons Network^™

A New All-Natural Wound Treatment Gel Shows Strong Inhibitory Activity Against Staphylococcus Aureus And Other Wound Pathogens, Tasha K. Nelson

Electronic Theses and Dissertations

Skin related injuries are some of the most dangerous forms of wounds. In addition to treating the wound itself, health care providers must be cautious of microbial infections. In this study, we evaluate a novel all-natural antimicrobial gel compound (AMG) designed to kill planktonic bacteria, penetrate bacterial biofilms, and accelerate wound healing. In -vitro experiments demonstrate that AMG is effective in inhibiting planktonic growth and biofilm development of eight common pathogens. LIVE/DEAD staining and confocal microscopy reveal that planktonic growth and three-dimensional structure of biofilms were significantly reduced. Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) was used to investigate a small …

Investigating A Novel Function For Phosphoserine Aminotransferase 1 (Psat1) In Epidermal Growth Factor Receptor (Egfr)-Mediated Lung Tumorigenesis., Rumeysa Biyik-Sit

Electronic Theses and Dissertations

Phosphoserine aminotransferase 1 (PSAT1) catalyzes the second enzymatic step within the serine synthetic pathway (SSP) and its expression is elevated in numerous human cancers, including non-small cell lung cancer (NSCLC). Epidermal growth factor receptor (EGFR) mutant NSCLC is characterized by activating mutations within its tyrosine kinase domain and accounts for 17% of lung adenocarcinomas. Although elevated SSP activity has been observed in EGFR-mutant lung cancer cells, the involvement of PSAT1 in EGFR-mediated oncogenesis is still unclear. Here, we explore a putative non-canonical function for PSAT1 using biochemical approaches to elucidate unknown interacting proteins and genomic RNA-seq profiling to identify cellular …

Harnessing The Power Of Trained Immunity In The Setting Of Pancreatic Cancer: A Novel Mechanism Of Immune Trafficking And Tumor Control., Anne Elena Geller

Electronic Theses and Dissertations

Despite the success of immunotherapy in many types of cancer, pancreatic adenocarcinoma (PDAC) has yet to benefit. Innate immune cells are critical to antitumor immunosurveillance and recent studies have revealed that these populations possess a form of memory, termed trained innate immunity, which occurs through transcriptomic, epigenetic, and metabolic reprograming. Though trained innate immunity has mostly been investigated in the context of infection, the induction of trained innate immunity could also protect against tumors, and specifically pancreatic tumors. Here, we demonstrate that yeast-derived particulate β-glucan, a known inducer of trained immunity, traffics to the pancreas following IP administration. This causes …

Identifying The Cardiovascular Effects Of Multiple Pollutants., Katlyn Elizabeth Mcgraw

Electronic Theses and Dissertations

Cardiovascular disease (CVD) is the leading cause of death from environmental exposures. Although exposure to PM_2.5 is an established risk factor for CVD, the contribution of other hazardous pollutant exposure to CVD is less clear. Overall, this work aimed to examine the effect of pollutants with lesser documented effects on cardiovascular disease using a multi-pronged approach to exposure assessment. The three aims were to examine the relationship between county-level toxic chemical releases and CVD mortality in the contiguous United States between 2002 and 2012, to assess the relationship between individual-level VOC metabolites and vascular function, and to build multipollutant …

The Role Of Src In Nsclc Cell And Lung Fibroblast Migration On 3d Cell-Derived Matrix., Austin M Krueger

Electronic Theses and Dissertations

Lung cancer is responsible for the most cancer deaths between males and females, with a 6% five-year survival rate. Cancer metastasis is one of the leading causes of lung cancer lethality. Our lab has developed a method to culture cells on cell-derived matrix (CDM) and study cell-ECM interactions. This study utilized multiple cell migration methods to investigate the migration of NSCLC cells and IMR90 lung fibroblasts, both together and independently, on 2D plastic and 3D CDM. Our results showed that inhibiting Src, a significant influencer of cell migration and member of integrin activation, blocked cancer cell migration on 2D and …

Pde4 Inhibition: A Novel Therapeutic Strategy In Liver Fibrosis., Mohamed Elnagdy

Electronic Theses and Dissertations

Background: Liver fibrosis is accumulation of extracellular matrix (ECM) proteins due to chronic liver injury. Chronic hepatic damage can occur due to multiple causes including, alcohol, non-alcoholic steatohepatitis and chronic viral hepatitis. Liver fibrosis is a critical problem worldwide due to the extremely high incidence of alcohol associated liver damage and chronic viral hepatitis. Moreover, there is no FDA approved therapy for liver fibrosis. Activation and transdifferentiation of quiecent hepatic stellate cells (HSCs) to myofibroblasts (MFBs) is the main event contributing to liver fibrosis. This process involves two phases, the initiation and the perpetuation phases. Hepatocyte injury and inflammatory response …

Correctional Officers' Response To Stress: An Exploration Of The Associations Between Violence And Trauma Exposure, Coping Mechanisms, And Ptsd., Katie Lorraine Hughes-Taylor

Electronic Theses and Dissertations

The current study examines correctional officer coping mechanisms and whether they impact the likelihood of an officer developing PTSD symptoms. Additionally, research questions focus on whether problem-focused or emotion-focused coping mechanisms moderate the relationship between exposure to trauma and PTSD symptoms. The data for the current dissertation comes from a larger National Institute of Justice grant determined to examine mindfulness as an intervention for correctional officer PTSD within Kentucky Department of Corrections. Approximately 245 officers from seven adult institutions participated in the study and completed a lengthy survey. Main variables for the current analysis include two dependent variables, both of …

Re-Visiting The Flutie Effect: An Exploration Of Athletic Success' Impact On Student Enrollment Decisions., Addison Pond

Electronic Theses and Dissertations

With each passing year, FBS collegiate athletic departments are faced with increased expenditures (Huml et al., 2019). Subsequently, institutional funding has become an increasingly common and controversial method of athletic department funding (Jewell, 2020). These spending and subsidization patterns are commonly met with controversy, causing the need to further unpack the benefits that schools receive from these behaviors. One anticipated benefit of athletic success is increased numbers of student applications. While the impact of football and basketball success on student interest (the “Flutie Effect) has been a popular topic in sport management literature, there is a lack of primary data …

Heterocyclic Amines And Arylamine N-Acetyltransferase 2 Polymorphism In Pathogenesis Of Insulin Resistance., Kennedy M. Walls

Electronic Theses and Dissertations

Heterocyclic amines (HCAs) are formed when cooking meats at high temperatures or for long periods of time, and their major metabolic pathway includes hepatic N‑hydroxylation by CYP1A2 followed by O-acetylation by N-acetyltransferase 2 (NAT2). NAT2 is a highly polymorphic gene involved in metabolism of drugs and xenobiotics, and its single nucleotide polymorphisms (SNPs) produce phenotypes characterized as slow, intermediate, or rapid acetylator. Recent studies have documented associations between HCAs and insulin resistance and between certain NAT2 phenotypes and insulin resistance. In this study, we demonstrated that NAT2 activity and exposure to certain HCAs may alter cellular glucose …

Defending Judge Roy Moore: A Case Study Of Persuasion Resistance Strategies., Stephen F. Brockman

Electronic Theses and Dissertations

This study explored what kind of persuasive resistance strategies people used when faced with information that was contrary to their existing belief system. A typology of resistance strategies as articulated by Fransen, Smit, & Verlegh (2015), was used to guide the development of a coding system. I coded the public quotes of supporters of a political candidate after sexual misconduct allegations came to light. The first research question investigated was: What kinds of persuasion resistance strategies were Roy Moore supporters most likely to use? The second was: What kinds of persuasion resistance strategies used tended to co-occur? The most important …

Acting On What We Hear: The Impact Of A Listening Methodology In A Community Literacy Program., Megen Farrow Boyett

Electronic Theses and Dissertations

Calls for better listening, especially to racially and linguistically marginalized populations, are popular and necessary in community literacy studies. However, while scholarship has clarified the need for attending to a range of voices across the life of a community program and demonstrated clear results of good listening, it has not sufficiently outlined a methodology for listening, especially as it applies to community-engaged action. Even less scholarship has focused on attending to current listening practices, so that scholars and community partners hoping to practice active listening interpret participants’ input through a soundtrack of other, unnoticed, “voices,” and have no way of …

Determination Of The Biological Functions Of Undefined Domains Of Veev Nsp2., Andrew Michael Skidmore

Electronic Theses and Dissertations

Alphaviruses are positive sense, single strand, RNA viruses. These viruses occur on every populated continent. Alphaviruses are divided into two clades, the New-World and Old-World viruses. The New-World viruses include Eastern (EEEV), Western (WEEV), and Venezuelan equine encephalitis viruses (VEEV), and cause neuroinvasive disease. The Old-World viruses include Chikungunya (CHIKV) and Ross River viruses (RRV), and typically cause multijoint arthralgia. There are currently no approved antiviral therapeutics or vaccines for any alphavirus, making them a high priority for antiviral drug design and discovery. A benzamidine inhibitor (ML336) of VEEV was characterized, and determined to inhibit replication of VEEV RNA during …

Incidental Exposure, Political Activity And Perceived Trust., Brianna Berry

Electronic Theses and Dissertations

The current study examines the concept of incidental versus traditional exposure to information through the context of a police brutality incident. Incidental exposure on social media is when a person is exposed to information or imagery without prior warning. It is hypothesized that 1) individuals who are incidentally exposed to a graphic police brutality event will be more likely to participate in politics and 2) will have lower perceived trust in the government. This randomized study utilizes two treatments (incidental exposure and traditional exposure) and a control group. Analysis of the data shows that support for H₁ is only …

Shifting Sands., Rachid Tagoulla

Electronic Theses and Dissertations

Shifting Sands is a re-exploration of the presentation of North Africans in colonial postcards, an examination of identity, and a critique of the modern Western museum. Since the inception of photography, colonizers used this medium- especially in the form of postcards- to categorize and exoticize Eastern peoples in order to more easily subjugate them. Shifting Sands is a series of reconstructed colonial postcards which challenges colonial-era stereotypes of North African peoples. The colonial gaze, represented by the camera lens, is subverted through a lensless image-making process in which sand is used to remove the subject from the colonial gaze and …

Ga-Based Iii-V Semiconductor Photoanodes For Solar Fuels And Novel Techniques To Investigate Their Photocorrosion., Sahar Pishgar

Electronic Theses and Dissertations

Solar energy is one of the most abundant renewable energy sources. However, the diurnal variation of the sun as well as seasonal and weather effects, limits the widespread global implementation of solar energy. Thus, Cost-effective energy storage is critical to overcome the intermittent nature of solar energy available on the earth. Photoelectrolysis of water to oxygen and hydrogen fuel is a promising large-scale solution to store intermittent solar energy in a dense and portable form. Photoelectrochemical (PEC) water-splitting, or artificial photosynthesis, research strives to develop a semiconductor photoelectrode with both high efficiency and long-term stability. Semiconductors of the III–V class …

Isomotive Dielectrophoresis For Enhanced Analyses Of Cell Subpopulations., Mohamed Zakarya Rashed

Electronic Theses and Dissertations

As the relentless dream of creating a true lab-on-a-chip device is closer to realization than ever before, which will be enabled through efficient and reliable sample characterization systems. Dielectrophoresis (DEP) is a term used to describe the motion of dielectric particles/ cells, by means of a non-uniform electric field (AC or DC). Cells of different dielectric properties (i.e., size, interior properties, and membrane properties) will act differently under the influence of dielectrophoretic force. Therefore, DEP can be used as a powerful, robust, and flexible tool for cellular manipulation, separation, characterization, and patterning. However, most recent DEP applications focus on …

Identification Of Transcription Regulators Of The Algz/R Two-Components Regulatory System In Pseudomonas Aeruginosa, Kwasi Yeboah

Electronic Theses and Dissertations

Pseudomonas aeruginosa is an opportunistic pathogen that express a plethora of virulence components controlled through two-component regulatory systems that allow for sensing and responding to environmental stimuli. This study was aimed at identifying transcription regulators of algZ that encodes the histidine sensor kinase (AlgZ) of the AlgZR two-component regulatory system. To understand how the algZ gene is transcriptionally controlled, transposon mutagenesis was used to create a mutant library with varying algZ expression based on their b-Galactosidase activity. The gene PA3327 was identified as a potential regulator of algZ expression using arbitrary PCR. This gene encodes a probable non-ribosomal peptide synthetase …

How Nutrition Knowledge Of Coaches, Athletic Trainers, And Strength And Conditioning Specialists Translates To Their Athletes, Alexa Carbone

Electronic Theses and Dissertations

The basis of dietary habits is linked to an individuals’ nutrition knowledge; for collegiate athletes this is the difference between optimal performance and falling short. To understand how nutrition information is disseminated to student-athletes, this research surveyed the athletes themselves and their sports staff (coaches, athletic trainers, and strength and conditioning specialists) on their current knowledge. Sport staff and student-athletes at East Tennessee State University were given the opportunity to participate in a 52-question online survey that tested both their general and sports-related nutrition knowledge. While the study sample size did not allow for statistical analyses required to address all …

Vitamin B12 Deficiency Does Not Stimulate Amyloid-Beta Toxicity In A Ceanorhabditis Elegans Model Of Alzheimer’S Disease, Opeyemi F. Showemimo

Electronic Theses and Dissertations

Alzheimer’s disease (AD) is symptomized by amyloid-beta plaques in the brain and accounts for more than 65 percent of dementia cases. Vitamin B12 (cobalamin) deficiency can result in similar cognitive impairment and roughly 15% of the elderly are vitamin B12 deficient. Vitamin B12 deficiency results in the accumulation of toxic methylmalonic acid and homocysteine. Hyperhomocysteinemia is a strong risk factor for AD. To test if vitamin B12 deficiency stimulates amyloid-beta toxicity, Caenorhabditis elegans expressing amyloid-beta in muscle were fed either vitamin B12-deficient OP50-1 or vitamin B12-rich HT115(DE3) E. coli bacteria. Increased amyloid-beta toxicity was found in worms fed the …

The Impact Of Artificial Intelligence In The Customer Journey: A Case Study Of Bosch Usa And Defy South Africa, Tariro S. Munyengeterwa

Electronic Theses and Dissertations

Artificial intelligence (AI) continues to gain traction and is increasingly reshaping the media and marketing communications field. While significant research has been conducted on the impact of AI in other fields, there is little empirical evidence on how AI is affecting the customer journey. The present study explored both organizations’ current use of AI tools and how customer perceptions about AI affect AI usage and adoption through the lens of diffusion of innovation theory. The research was conducted using mixed-method qualitative research. In-depth interviews and a case study content analysis were conducted to collect and analyze the data. The results …

Profiling Beyond Race: Characteristics Associated With Traffic Stop Outcomes, Megan Anderson

Electronic Theses and Dissertations

Research related to profiling and the outcome of traffic stops has generally focused on the race of the individuals involved. Little research has examined other characteristics, such as age and socioeconomic status, that may also play a role in traffic stop outcomes. The current study sought to address this limitation in two ways: (1) determine whether the characteristics of age, sex, race, social class, and demeanor are profiled during traffic stops and (2) whether these characteristics influenced the outcome of the traffic stops with regard to tickets and vehicle searches. Secondary data were utilized from the 2015 Police-Public Contact Survey. …

Teacher Perception Concerning The Role Of Elementary Principals In School Culture And Climate, Robyn Murray

Electronic Theses and Dissertations

A phenomenological research study was conducted to examine teacher perceptions of elementary principals’ behaviors affecting school culture and climate. The researcher gathered data from participant interviews. Participants were selected using purposeful sampling of teachers identified by their principals as meeting study criteria. The schools in which teachers were selected were also chosen using purposeful sampling based on superintendents’ perceptions of positive principal influence on school culture and climate.

Data analysis provided insight to the researcher into the phenomenon being explored in this study. Teachers were asked to participate in an individual interview with the researcher and provide answers to open …

Hemin Utilization In Rhizobium Leguminosarum Atcc 14479, John Lusby

Electronic Theses and Dissertations

Rhizobium leguminosarum is a Gram negative, motile, nitrogen-fixing soil bacterium. Due to the scarcity of iron in the soil bacteria have developed a wide range of iron scavenging systems. The two types of iron scavenging systems used are indirect and direct. In-silico analysis of the genome identified a unique direct iron scavenging system the Hmu operon. This system has been identified in other closely related rhizobium species and is believed to be involved in utilizing heme compounds as a sole source of iron. We have attempted to characterize the role of the Hmu operon in iron utilization by monitoring the …

Black And White Student Achievement Gaps In Tennessee, Haley Dirmeyer

Electronic Theses and Dissertations

Achievement gaps between Black students and White students have existed since public education was desegregated, and they still persist despite efforts to close the gap. This research describes the achievement gaps between Black and White 3^rd through 8^th grade students in the state of Tennessee from 2017-2019. This is a non-experimental, quantitative, comparative-analysis describing the ELA and math test scores of Black students and White students in each of the three geographic regions of Tennessee. Data were arranged in 2x2 contingency matrixes to compare the expected frequencies of students in each race scoring on-track and mastered versus below …

Generation X And Generation Y: An Exploration Of Student Motivation To Learn And Technology Use, Ladonna Hutchins

Electronic Theses and Dissertations

Student motivation and technology use are important considerations for higher education institutions. With increasing proportions of institutional funding being tied to student success and retention outcomes, gaining an awareness of how students tend to be motivated as well as their comfort and skill level with technology is critical for supporting student success in the collegiate classroom. The purpose of this study was to examine motivations for learning and technology use by specific generations, Generation X and Generation Y, among participants in two learning settings, a four-year university and a two-year community college. Differences in motivation type including intrinsic, extrinsic, and …

The Effects Of Virtual Nature Exposure On State Social Motivation, Samantha A. Castelblanco

Electronic Theses and Dissertations

Social health is an important predictor of overall health. Yet, it is an often neglected area of research. Strikingly, social connectedness is associated with a 50% reduction in risk of early death. While a plethora of research evidence supports the beneficial impact of nature exposure on physical and mental health, literature regarding the beneficial impact of nature exposure on social health is scant. In fact, no research to date has investigated the causal influence of nature exposure on social motivation, a construct comprised here of three measures (State Motivation to Foster Social Connections, State Positive Affect, and State Anxiety). The …

Intrinsic Religiousness And Its Relation To Health Outcomes, Joseph Barnet

Electronic Theses and Dissertations

Religiousness, broadly defined, has been shown to be predictive of a variety of health outcomes. Past literature surrounding religiousness research has utilized different definitions and measures for the meaning of religiousness. How religiousness is defined can influence its relationship in relation to health outcomes. The present study utilized a measure for intrinsic religiousness, which is defined as an internalization of the tenets of a particular faith. The present study examined whether intrinsic religiousness predicts problematic or illicit substance use or pornography use in a sample of participants that included mostly undergraduate students from the Appalachian region, as well as some …

Personification In Advertising: A Rhetorical Analysis Of Digital Video Ads In The Insurance Industry, Dorm Kpedor

Electronic Theses and Dissertations

Major companies in the insurance industry—notably Allstate, Progressive, and Farmers—often employ personification as a creative rhetorical tool in digital video advertisements. By leveraging brand characters in various ways, these companies seek to establish trust and engender emotional impact in customers. Allstate ascribes destructive characteristics that are associated with house cats to its Mayhem character; in doing so they evoke the desired emotional responses of humor and fear. Progressive creates and deploys the Motaur character, a visual personification and play on the Centaur; in this case, the company’s rhetorical strategy is to evoke humor and nostalgia that resonate with motorcycle owners. …

Character Influence And Brand Relationships: How Morally Ambiguous Characters Shape Brand Identity?, Jason Alexander Daugherty

Electronic Theses and Dissertations

This study examines morally ambiguous characters in television dramas and they represent their respect brand. The goal of the study is to examine how these MACs define their respective brands, specifically the brands, AMC and Showtime. Recent studies observed people chose brands based on their affective disposition and social cognition towards specific influencers. These theories could inform how people relate to characters from TV shows and brands. An observation and narrative analysis of four characters promotional videos from AMC were Don Draper (Mad Men), Saul Goodman (Better Call Saul) and from Showtime Jackie Peyton (Nurse Jackie) and Dexter Morgan (Dexter). …

Investigating The Association Between Adolescent Polysubstance Use, Crime, And Violence In The United States, Christian Nwabueze

Electronic Theses and Dissertations

The lifetime prevalence of alcohol in the United States is 8% and lifetime prevalence of illicit drug use is about 2-3%. Substance use is common among adolescents and polysubstance use is becoming a public health threat.

The prevalence of adolescent physical dating violence was 8.2%, the prevalence of adolescent sexual violence was also 8.2% while the prevalence of dual adolescent physical and sexual violence was 2.6%. Compared to those who used only single substances, adolescents who did not use any substance were 60% less likely (OR = 0.42, 95% CI = 0.24 – 0.74) to experience physical dating violence, 50% …