Real-Time Monitoring Of Cell Death Progress Using Capacitance Spectroscopy, Suyang Wu

Electronic Theses and Dissertations

Biologics, including the monoclonal antibody (mAb), has experienced rapid development in the last decade. However, the price of biologics is often prohibitively high because of the low process efficiency. Delaying the inevitable cell death improves the productivity of upstream bioprocessing, whose success relies on monitoring the cell death onset that indicates the timing for preventive actions.

This study proposes to develop a real-time monitoring model that quantifies the dying cell percentage in lab-scale bioreactors using capacitance spectroscopy. The capacitance spectroscopy contains cell death-related information due to various physical properties changes during the cell death process, e.g., cytoplasmic conductivity change. The …

Understanding Snow Leopard (Panthera Uncia) Population Structure, Diet, And Human-Wildlife Dimensions Using Noninvasive Genetic Approaches, Charlotte Hacker

Electronic Theses and Dissertations

Snow leopards (Panthera uncia) are a large cat endemic to the mountains of Central Asia. Despite their status as a flagship species, there are numerous knowledge gaps surrounding their population status, dietary ecology, and relationship with humans. Such knowledge gaps are prohibitive for effective conservation action for the species. Snow leopards are threatened by habitat loss, decreased prey availability, retaliatory killings, and large-scale negative impacts associated with climate change. In order to help bridge these gaps, noninvasive genetic methodologies were deployed in this work across several disciplines. Population genetic metrics were garnered at local, regional, and range-wide scales, …

Culturally Therapeutic Approaches To Prejudice And Discrimination: Formulations, Interventions, And Structural Change, Jayme J. Jenkins

Electronic Theses and Dissertations

Aims. Though psychologists are generally well-positioned to implement micro- and macro-level interventions from their roles as clinicians, educators, researchers, and consultants, there is a paucity of formal guidance on how to approach client-generated or structural prejudice and discrimination. The current study addresses this literature gap by integrating previous literature and implementation models from a related field toward a novel treatment approach.

Background. A critical literature review supported intervention-focused conceptualizations of “supremacism” and demonstrated that mutually reinforcing micro- and macro-level discrimination generates demographic disparities in safety, liberty, and well-being. This typically emerges in the United States as White supremacist heteropatriarchy while …

Seasonal Flammability Comparisons Of Native And Exotic Plants In The Post Oak Savannah, Blackland Prairie, And Pineywoods Ecoregions Of Texas, Michael Tiller

Electronic Theses and Dissertations

East Texas’ diverse landscape can present year-round wildfire seasons that can be influenced by seasonal and regional differences in climate and physiography. Greater insight into the fundamental thermal behavior of wildland fuels can aid in fire behavior prediction and development of fire-resistant plant lists. This study focused on estimating seasonal and regional flammability characteristics of five evergreen species: yaupon (Ilex vomitoria), Chinese privet (Ligustrum sinense), greenbrier (Smilax spp.), eastern red cedar (Juniperus virginiana), and escarpment live oak (Quercus fusiformis); and two deciduous species: Chinese tallow (Triadica sebifera) and southern …

The Political Act Of Writing Feminism- Begum Rokeya Sakhawat Hossain And The Utopian Vision, Mashall Momin

Electronic Theses and Dissertations

Feminist Begum Rokeya Sakhawat Hossain lived a life of seclusion and oppression like many middle-class Muslim women in colonial India during the late nineteenth and early twentieth century. During this period, Rokeya used writing as a tool to fight against the oppression women faced from the patriarchal society and reimagined their gendered position in society. Rokeya wrote two novels, Sultana’s Dream and Padmarag, both set in feminist utopian societies. In these works, Rokeya expresses that the problem to the oppression of women can be traced to the purdah system or the seclusion of women, the solution is to grow …

Clean Fields, Lindsey E. Creel

Electronic Theses and Dissertations

Clean Fields is an exhibition consisting of 3 anthotypes and 11 drawings on paper mounted to panels. The work is part homage and part documentation of the emotionally restorative properties found in gardening. It is a contemporary view of a timeless activity. While the work references realistic content, the artistic outcomes are non-objective. Modifications in composition and scale provide variation throughout the series. The large-scale of the works and the use of economy and movement in the composition move the viewer into a meditative state.

An Exploration Of Afrocentric Features At A Black Homeschool Collective., Tytianna Nikia Maria Wells

Electronic Theses and Dissertations

This dissertation explores the development and benefits of familial relationships as the primary feature of Afrocentricity at Black Scholars Academy (BSA), a Pre-Kindergarten (Pre-K) through 12th-grade Black homeschool collective in the United States. This qualitative study draws upon Afrocentricity as a theoretical framework to analyze and interpret data collected from classroom observations, individual interviews with current and former teachers and students, and textual artifacts between July and November 2019. Familial relationships—an African time orientation, a personalized learning plan, OurStory, and Rising Meeting—are present in BSA. Familial relationships helped students develop cultural pride, agency, self-determination, independence, liberation through education, and a …

Manifestations Of Students’ Voices: Examining Shifts, Academic Demands, And Identity Work In How Students Make Themselves Understood., Lauren Elizabeth Fletcher

Electronic Theses and Dissertations

Voice is a concept that is both highly sought after and elusive in education. While schools aim to foster students’ voices, many academic structures inadvertently conceal their voices and in turn their identities. Definitions of voice have been assumed, vague, or looked at as a writing trait, with little consideration of voices’ dynamic and mediated structures. Drawing on scholarship grounded in sociocultural theories and dialogism (e.g., Bakhtin, 1986; Engeström; 1987, Leont’ev, 1981; Rosenblatt, 1978; Vygotsky, 1978), I contribute a new, tangible definition of voice, in which voice is a dynamic happening, continually negotiated and constructed. This dissertation explores students’ voices, …

The Uncanny Desire In American Animation And Film., Kristen Hankins

Electronic Theses and Dissertations

Although surrealism was short lived, the impact on popular culture is ongoing. Analyzing this impact is quite interesting because it set a standard for American animation and film. In this thesis, Freud’s theories of psychoanalysis and the uncanny are investigated through the lens of the camera in American cinema and animation. This thesis looks at cartoons and films that were made after Breton’s surrealist manifestos were published. This thesis aims to show the viewer the lasting impact that Breton had on American popular culture. This thesis is divided into three chapters with an introduction and conclusion. Chapter one discusses Fleischer …

Q-Griffithsin Interactions And Utility For The Prevention And Treatment Of Mucosal Infections., Henry Nabeta

Electronic Theses and Dissertations

Griffithsin (GRFT) is a carbohydrate binding agent (lectin) that was originally identified in the red alga Griffithsia sp. Q-Griffithsin (Q-GRFT) is an oxidation stable analog of GRFT. GRFT has demonstrated inhibitory activity against HIV-1, Coronaviruses, Hepatitis C, influenza and Ebola viruses. The broad-spectrum activity suggests the potential utility of this lectin in a wide range of viral infections. However, the lectin’s activity in mucosal infections has not been extensively studied. Using in vitro, ex vivo and in vivo assays, we have demonstrated that Q-GRFT maintains the ability to bind glycosylated ligands following incubation in murine, macaque and human rectal …

Effects Of Chemosensory Experience And Context On Consummatory Behaviors., Saphira M. Chiu

Electronic Theses and Dissertations

Eating food generates associations between odors and tastes (i.e., flavor) that guide future choices. Experience with an odor-taste mixture links an odor with a taste’s quality and hedonic value, resulting in a preference for an odor paired with a palatable taste over an odor paired with an unpalatable taste. However, experience with a neutral stimulus (i.e., latent inhibition) or environment (i.e., context) can influence the formation of conditioned associations. Here, I used a two-bottle brief-access task to determine whether rats display an innate preference between unpaired odors (isoamyl acetate and benzaldehyde), how preexposure to the unpaired odors impacts mixture-dependent consummatory …

Social Cognition, Impulsivity, And Emotion Regulation Factors In Aggressive Behavior Among Children With Attention-Deficit/Hyperactivity Disorder., Kelly E. Slaughter

Electronic Theses and Dissertations

Children with Attention-Deficit/Hyperactivity Disorder (ADHD) exhibit greater levels of aggressive behavior than their typically developing peers, often resulting in impairment in social and family functioning. Aggressive behavior is often differentiated into two functions: reactive, or “hot-blooded” and proactive, or “cold-blooded” aggression. Prior research has identified several factors contributing to aggressive behavior within a general population, including emotion dysregulation, negative urgency, social information processing (SIP), and parenting behaviors. A paucity of research has examined these factors within an ADHD population. Thus, the present study aimed to examine social, emotional, behavioral, and cognitive factors associated with aggression among children with ADHD. Specifically, …

Social And Academic Experiences Of Black, First-Generation, College Graduates While Attending Predominantly White Institutions, James Edward Stukes

Electronic Theses and Dissertations

First-generation college students represent a significant percentage of all college students and comprise various sub-identities and unique backgrounds. Compared to continuing generation college students, many first-generation students arrive on college campuses without complete understanding of how to navigate college life. Factors, such as family support, finding a sense of belonging, and the availability of resources and campus support, play key roles in the overall success of first-generation college students. The current study sought understanding of these aspects of college life and their relationship to the success of Black, first-generation, graduates of predominantly White institutions.

The purpose of this qualitative, phenomenological …

Evaluation Of Risk And Protective Factors Of Substance Use In The National Association Of Intercollegiate Athletics., Richard D Weaver Jr.

Electronic Theses and Dissertations

The NCAA and NAIA have conducted prevalence studies of substance use in their organizations but little research into risk and protective factors that influence usage. Substance use is associated with an array of consequences including negative academic impacts, criminal charges, health risks, and mental health. As the most reported substances used by student-athletes the study focused on alcohol, marijuana, and nicotine. This study specifically examined several risk and protective factors impacting substance use. This study utilized a secondary data analysis of information gathered from the NAIA Substance Use and Abuse Study conducted in 2020 with student-athletes (N=2489). Descriptive statistics, parametric …

Developing Digital Literacy In Digital Natives: A Quantitative Study Of Digital Literacy And Niswonger Online Students, Elizabeth Pavlovich

Electronic Theses and Dissertations

This non-experimental, quantitative study was completed to determine if Tennessee high school students who take an online, asynchronous course better their digital literacy through the environment of online learning. The study focused on the pretest and posttest scores of Niswonger Online students during the spring and summer semesters of 2021. It grouped the high school students by location, high school size, grade level, and courses taken to assess differences in digital literacy. Finally, it evaluated overall student growth in digital literacy scores after completion of an online high school course.

Data from a pretest and posttest of Niswonger Online students …

Trained Immunity Enhances The Immune Response And Maintains Microbiome Diversity In Aging And Sepsis, P. Spencer Gill

Electronic Theses and Dissertations

The global population is rapidly aging. It is estimated that over the next thirty years, the number of individuals >60 years of age will increase by over a billion, and the number of individuals over age 80 may increase by 300 million. As humans age, our immune system becomes progressively weaker through a process called immune senescence. This age-related decrease in immune function increases susceptibility to infection and chronic diseases. Sepsis is a leading cause of death worldwide. Over the past two decades, there has been an increased incidence of sepsis which is due, in part, to our aging population …

Nonprofit Narratives: How Two Organizations Use Social Media And Rhetorical Appeals To Address Issues Of Sexual And Domestic Violence, Samuel Hiester

Electronic Theses and Dissertations

Though often seen as a panacea for organizational objectives, nonprofits must be judicious in deploying social media, particularly due to resource limitations. Nonprofits deploy many types and styles of digital texts, including social media. Classical rhetorical appeals can be effective means for achieving positive impact in that context. When used correctly, these ‘digital texts’ can be leveraged for maximum engagement with audiences. This study examines both a large, national organization – the National Sexual Violence Resource Center – and a small, regional one – Branch House Family Justice Center – for not only what sort of digital texts are utilized, …

Functional Mixed Data Clustering With Fourier Basis Smoothing, Ishmael Amartey

Electronic Theses and Dissertations

Clustering is an important analytical technique that has proven to affect human life positively through its application in cancer research, market segmentation, city planning etc. In this time of growing technological systems, mixed data has seen another face of longitudinal, directional and functional attributes which is worth paying attention to and analyzing. Previous research works on clustering relied largely on the inverse weight technique and B-spline in smoothing data and assessing the performance of various clustering algorithms. In 1971, Gower proposed a method of clustering for mixed variable types which has been extended to include functional and directional variables by …

Familial Regulation Of Young Children's Tv Viewing In Ghana, Clara Puni-Nyamesem

Electronic Theses and Dissertations

This study examined familial regulation of young children's TV viewing in Ghana. Participants were families with young children four through eight years enrolled at a school in the south of Ghana. An online survey of TV regulation practices of families (restrictive, coviewing, and instructive), TV viewing hours of young children, and families' perception of TV influence on their children was completed by 158 family members (mostly parents). Results showed that restrictive regulation is the most predominately used strategy in Ghana, although all 3 regulation styles were used. College educated families were significantly more likely to use restrictive regulation than families …

All Roads Lead To Darrington: Building A Bluegrass Community In Western Washington, James W. Edgar

Electronic Theses and Dissertations

Through the mid-twentieth century, a significant pattern of migration occurred between Appalachia and the Pacific Northwest, with Washington’s thriving timber industry offering compelling economic opportunities. Many workers and families from western North Carolina settled in the small mountain town of Darrington, Washington, frequently accompanied by their banjos and guitars. As a group of young bluegrass enthusiasts from Seattle established relationships with Darrington’s “Tar Heel” musicians, a collaborative music community formed, laying the foundation for the region’s contemporary bluegrass scene.

Drawn from a series of ethnographic interviews, this project illuminates the development of a bluegrass community in western Washington, while identifying …

Facile Nitrogen-Doping Of Screen-Printed Carbon Electrodes For Detection Of Hydrogen Peroxide, Emmanuel Nkyaagye

Electronic Theses and Dissertations

Screen-printed carbon electrodes (SPCEs) have garnered much attention as sensors due to their simplicity and relatively low cost. However, to impart necessary selectivity and sensitivity for specific applications, modification of the SPCE surface, which can involve time-consuming procedures or costly equipment/materials, is typically required. Here, a simple nitrogen-doping process based on NH₄OH was used to modify SPCEs prepared from commercially available ink for electrochemical detection of H₂O₂, a common target for biosensing strategies and indicator of cell stress. XPS studies showed that NH₄OH treatment of SPCEs led to a nearly 5-fold increase …

Movies That Sell: A Rhetorical Analysis Of Product Placements In Marvel Movies, Andrew Nii Okai Okai

Electronic Theses and Dissertations

The advancement of digital entertainment media has given audiences the ability to skip ads that do not interest them. Consequently, brands face the challenge of creating ads that can compel audiences and finding media outlets that can effectively reach target audiences. Brands today use product placements to promote their products because movie audiences are generally attentive to ads when they are incorporated in a movie’s narrative. Marvel Cinematic Universe is a globally recognized entertainment franchise that uses product placements strategically to promote brands in their movies and TV shows. In this study, I conduct rhetorical analyses of select product placements …

Developing A Process To Select B2b Target Markets With A Focus On Siemens Simatic Controllers, Eva Limbrunner

Electronic Theses and Dissertations

The process to identify and describe target markets can be challenging for marketers due to the complexity of B2B markets and industrial products. This case-based study aims to bridge the gap between theory and praxis by developing a process to determine target markets for Siemens SIMATIC controllers, which are highly technological products designed for process control and used in industrial applications. The author conducted a literature review to investigate the current understanding of B2B market segmentation and target marketing and collected qualitative data from Siemens. This study defines segmentation variables leveraging segmentation models as a foundation to build and describe …

Lipidomic Analysis Of Various Developmental Stages Of Physcomitrium Patens, Deepshila Gautam

Electronic Theses and Dissertations

Lipids maintain fluidity of the cell membrane during the lifetime of all organisms. The moss Physcomitrium patens, an early land plant, enters reproductive phase under cold (15°C) conditions relative to its gametophytes (22°C). Thus, we hypothesized that their lipid content and composition would be distinct. Using ESI-MS/MS, we showed that the content and acyl composition of 11 lipid classes varied during development. Galactolipids were abundant in gametophytes but insignificant in sporophytes; among phospholipids, phosphatidylcholine was predominant in both phases. Although, sporophytes contained around five-fold less lipids than the gametophyte, their phosphatidic acid content, which accumulates during stress, was 18-fold …

Traditional And Non-Traditional Gender Role Stereotypes In Children’S Animated Films, Rachael Davidson

Electronic Theses and Dissertations

As gender stereotypes could lead to adherence to rape myths later in life, it is important to study the potential development of gender role stereotypes. Based on the theoretical approach of Bandura’s social learning, this mixed methods study sought to expand the literature on children observing gender stereotypes through film viewing. A content analysis of verbal and body language of the highest grossing animated films between 2017-2019 was conducted. The results indicated that most main characters displayed both traditional and non-traditional gender role stereotypes, which is indicative of gender role flexibility. This shows promise that there could be a moderating …

Electronic Evidence Locker: An Ontology For Electronic Evidence, Daniel Smith

Electronic Theses and Dissertations

With the rapid growth of crime data, overwhelming amounts of electronic evidence need to be stored and shared with the relevant agencies. Without addressing this challenge, the sharing of crime data and electronic evidence will be highly inefficient, and the resource requirements for this task will continue to increase. Relational database solutions face size limitations in storing larger amounts of crime data where each instance has unique attributes with unstructured nature.

In this thesis, the Electronic Evidence Locker (EEL) was proposed and developed to address such problems. The EEL was built using a NoSQL database and a C# website for …

The Influence Of Social Media On The Tourism Industry: A Content Analysis Of Culinary Tourism Brands Via Instagram, Angela White

Electronic Theses and Dissertations

The tourism industry in the United States is constantly changing and being influenced by social media, specifically Instagram. Culinary brands use Instagram to advertise their restaurants and dishes in a creative, visual way. As a result, Instagram is now being used as a tool by tourists and both potential and current customers to share their dining experiences and find these locations based on the visual appeal. This study provides a content analysis of Instagram account information and photo composition within eight popular culinary tourism destinations by examining the strategies used by the accounts. 50 randomly selected photos were used from …

Ultrafast Magnetic Entropy Dynamics With Time-Resolved Pump-Probe Magneto-Optical Technique., Sahar Goharshenasanesfahani

Electronic Theses and Dissertations

It has been observed that ultrathin films, multilayers, or magnetic nanostructures indicate novel magnetic phenomena that differ profoundly from the respective bulk properties. Besides, because of the broad applications of these magnetic materials in the industry, they are an exciting research area. Hence, investigating the low-dimensional magnetic systems is one of the most active fields in experimental condensed matter physics. Magnetization dynamics can occur over a wide range of time scales (from seconds to femtoseconds). Some of these processes even occur on time scales as short as a few picoseconds (10^-12s) or femtoseconds (10^-15s). Measurement of …

Theoretical Investigation Of The Co-C Bond Activation In Methylcobalamin And Adenosylcobalamin-Dependent Systems: Mechanistic Insights., Arghya Pratim Ghosh

Electronic Theses and Dissertations

The vitamin B₁₂ derivates, otherwise known as cobalamin (Cbl), are ubiquitous organometallic cofactors. The biologically active forms of Cbl, such as methylcobalamin (MeCbl) and adenosylcobalamin (AdoCbl), act as cofactors in different physiological reactions for both prokaryotes and eukaryotes. A crucial aspect of the Cbl-mediated systems is the activation of the organometallic Co-C bond that plays a critical role in its catalytic activity. One of the most remarkable features of this Co-C bond is its unusual activation in AdoCbl-dependent enzymatic reactions, where a trillion-fold rate acceleration of the Co-C bond cleavage is observed inside the enzyme compared to the isolated …

A Phenomenological Study Examining How The Covid-19 Pandemic Changed The Way Teachers Use Technology To Deliver Instruction From March 2020 - May 2021, Dedra Lamb

Electronic Theses and Dissertations

The purpose of this qualitative study was to examine teacher perception of changes in the instructional delivery and learning opportunities via technology during and throughout the COVID-19 pandemic from March 2020 until May 2021. The COVID-19 pandemic has greatly affected educational organizations. School closures in March 2020 forced teachers to change their instructional delivery from an in-person platform to a virtual platform. This disruption to the delivery of instruction with the use of technology changed the way teachers plan for learning, delivery content, present learning activities, and assessment. The urgency required teachers to develop new strategies and experiment with adaptations …