Digital Commons Network^™

Label-Free Biochemical Recognition Using Mems Resonators For Microarray Technology, Babak Tousifar

Electronic Theses and Dissertations

Highly sensitive biosensors capable of detecting and characterizing smallest quantities of cellular and molecular targets are needed in pharmaceutical and medical diagnostics industries. In this work, the importance of biological target recognition specifically proteins through microarray technologies has been discussed and the most successful tools and techniques have been studied. Moreover, a thermally actuated Micro Electro-Mechanical Systems (MEMS) resonator has been demonstrated and fabricated in this work as an accurate, reliable and low cost biotechnology tool. As a proof of concept, amine to epoxide coupling of octadecylamine to functionalized silicon dioxide surface have been shown through resonator frequency monitoring. The …

The Social Determinants Of Hiv/Aids In Cambodia: Is It Too Early To Call Micro Medical Efforts A Success?, Matthew Aaron Sherwood

Electronic Theses and Dissertations

Cambodia, a least developed country (LDC) with a recent history of civil war and genocide, has been widely considered to be a success story when it comes to the fight against HIV/AIDS. Within a few years of the first HIV positive blood test in the kingdom in 1991, it quickly became the country with the highest prevalence in the region, with estimates of as many as 4% of the population being infected at one point.

In recent years, Cambodia's HIV/AIDS infection rates have plummeted to below 1%, with most of the credit being given to the implementation of micro-medical measures, …

Maize And Stone A Functional Analysis Of The Manos And Metates Of Santa Rita Corozal, Belize, Lisa Glynns Duffy

Electronic Theses and Dissertations

The manos and metates of Santa Rita Corozal, Belize are analyzed to compare traditional maize-grinding types to the overall assemblage. A reciprocal, back-and-forth grinding motion is the most efficient way to process large amounts of maize. However, rotary movements are also associated with some ground stone implements. The number of flat and trough metates and two handed manos are compared to the rotary-motion basin and concave type metates and one-handed manos to determine predominance and distribution. Flat is the predominant type and, together with the trough type, these grinding stones make up the majority of metates at the site. Manos …

Design And Synthesis Of Novel Agrofungicides Based On Naturally Occurring Pbdes, Robert Alexander Smith

Electronic Theses and Dissertations

Cyanobacterial symbionts of Indo Pacific marine sponges, e.g. Dysidea sp., produce hydroxylated (OH-) polybrominated diphenyl ethers (PDBEs) which likely function as chemical deterrents owing to their antifungal and antibacterial properties. Limiting the therapeutic potential and agricultural use of these antimicrobial natural products are the negative connotations associated with non hydroxylated anthropogenic PBDEs. Anthropogenic PBDEs are pervasive global pollutants used as flame retardant additives in many household goods, e.g. furniture, textiles, and electronics. These molecules have a propensity to bioaccumulate in living organisms, they easily traverse the trophic food chains, they are readily passed from mother to offspring, and many of …

Starved: Food Deserts In The Mississippi Delta, Novelette L. Brown

Electronic Theses and Dissertations

This thesis examines the impact of food deserts on obesity, and builds a case for additional research on rural food deserts independent from urban ones. The Mississippi Delta consistently presents the highest obesity rates within the state, yet both the third unhealthiest county (Quitman) and the healthiest (DeSoto) are located in that region. One of the reasons there is such a large discrepancy between the health rankings of DeSoto and Quitman counties is that DeSoto is contained within the Greater Memphis Metropolitan Area, but Quitman is entirely rural. Previous research has focused on the prevalence of urban food deserts and …

Natural Products Chemistry Of Leads Acting On Mrsa, Hcv, Malaria, And Depression Disorders, Mohamed Ali Mohamed Ali Ibrahim

Electronic Theses and Dissertations

Nature has proven to be an endless source of abundant diversity of chemical entities with varying biological activities. The World Health Organization (WHO) estimates that 80% of the world's population depends on traditional medicine for treating their everyday health problems. According to Gordon Cragg, former director of the natural products branch of the NCI, ″Nature has produced wonderfully complex molecules that no synthetic chemist could ever dream up.″ Moreover, he emphasized that over 60% of cancer therapeutics have been derived from nature whether directly through isolation or indirectly through semi-synthesis. Various examples have been reported such as extracts from Catharanthus …

Development And Bioavailability/Bioequivalence Of A Fixed-Dose Combination Antiretroviral Reconstitutable Suspension Intended For Pediatrics, Fredrick Esseku

Electronic Theses and Dissertations

This dissertation was designed to develop and assess bioavailability/ bioequivalence of age-appropriate fixed-dose combination granules of lamivudine/zidovudine/nevirapine as reconstitutable suspension for pediatrics for use in pediatric patients. The granules were developed via roller compaction process to improve granule flow characteristics, minimize segregation of the multi-component active pharmaceutical ingredients, and avoid introducing moisture into the formulation and thereby improve the stability of the product. Optimization of the roller compaction process for the roll pressure and the ratio of the horizontal feed screw speed to the roll speed showed that these parameters had minimal effect on granule quality. Subsequently, a replicated 32 …

The Contribution Of Foundational New Testament Theological Themes To The Meaning Of Basic Bioethics Principles, R. Dennis Macaleer

Electronic Theses and Dissertations

Beauchamp and Childress' Principles of Biomedical Ethics is a well accepted approach to contemporary bioethics. Those principles are based on what Beauchamp and Childress call the common morality. This dissertation employs New Testament theological themes to enhance the meaning of contemporary principles of bioethics. Beginning with the incarnation in the New Testament, the invitation-response hermeneutic is developed as a hermeneutic to use in studying the New Testament. The primary New Testament text for this study is the twin commands from Jesus to love God and love one's neighbor. Three theological themes are developed from this study and these three themes …

Optimization Of Calcium Aluminate For Use As A Bone Scaffold Material Through Physical And Chemical Surface Modification, Rachelle Nicole Palchesko

Electronic Theses and Dissertations

Calcium aluminates were tested as biomaterials and show promise as bone scaffolds. Initial concerns of biocompatibility led the field to focus on calcium phosphates. Here, the work focused on improving the biocompatibility and creating an optimized calcium aluminate biomaterial. To accomplish this, the physical and interfacial properties of the material were varied. The physical properties of the calcium aluminate were varied through the room temperature casting procedure and five different mixtures were created and evaluated for mechanical strength and biotolerability. The results showed that the optimal mixture had an average pore size of approximately 100 µm and that autoclaving the …

A Paleopathological Assessment Of Osteoarthritis In The Lower Appendicular Joints Of Individuals From The Kellis 2 Cemetery In The Dakhleh Oasis, Egypt, Joshua B. Robin

Electronic Theses and Dissertations

Osteoarthritis (OA) is a degenerative pathological condition of the appendicular joints which affects the cartilage and underlying bone. OA is relatively common in both the archaeological and clinical context, and a significant amount of research has been conducted on this osteological condition. The purpose of this thesis is to assess the incidence, demographic prevalence, and general severity of hip and knee OA in a Roman-Christian period (50 A.D – 450 A.D) population sample from the Dakhleh Oasis, Egypt. The bioarchaeological sample originates from the Kellis 2 cemetery which is associated with the ancient town of Kellis. The town of Kellis …

The Innate Anti-Hiv-1 Activity Of Human Seminal Plasma, Julie A. Martellini-Moore

Electronic Theses and Dissertations

Human immunodeficiency virus (HIV) has become a global pandemic over the past few decades, with new infections and related deaths in the millions each year. There is no cure in sight for HIV-1 infection, and there has been little progress in developing an efficacious vaccine. Heterosexual transmission of HIV-1 remains the principal mode of transmission throughout the world and thus measures, such as topical vaginal microbicides, to prevent infection of the female reproductive tract are actively being explored. Recent trials of topical vaginal microbicides have shown that their interaction with the mucosal surfaces of the female reproductive tract as well …

Viability Study Of A Residential Integrated Stormwater, Graywater, And Wastewater Treatment System At Florida's Showcase Green Envirohome, Matthew Allen Goolsby

Electronic Theses and Dissertations

The subject of water scarcity and the rate of water consumption has become popular topics over the last few decades. It is possible that society may consume or contaminate much of the remaining readily available water if there is not a paradigm shift. This deep rooted concern has prompted investigations to identify alternative water use and treatment methods. Within this report, information is presented from the use of innovative water harvesting and on-site sewage treatment and disposal systems (OSTDS) at Florida’s Showcase Green Envirohome (FSGE.net), while also addressing low impact development (LID) practices. FSGE is a residential home that demonstrates …

Novel Approaches To The Control Of Infectious Diseases, Damaris Agathe Meujo Foping

Electronic Theses and Dissertations

As part of our ongoing efforts to find new drug leads against infectious diseases, several terrestrial and marine macro- and microorganisms were investigated. Several bioactive molecules, presented below, were isolated from these natural products. The mass-screening of dozens of marine Actinomycetes was completed and several Streptomyces with interesting biological activity profiles identified. A few examples are the Streptomyces code H747 and the Streptomyces code H668, a bacterium from which a new (5) and two known polyethers, the antimalarial agent K41-A (6) and its C-29 analog (7) were isolated. Five new six-membered ring cyclic peroxides: plakinastreloic acid A (8), methyl plakinastreloate …

Professional Preparation Of Secondary Health Education Teachers In Mississippi, Ensley Howell

Electronic Theses and Dissertations

The purpose of this study was to examine the professional preparation of secondary health education teachers in Mississippi. All four-year institutions of higher learning were contacted to determine the requirements for an approved program in order to obtain a supplemental endorsement in health, to compare the similarities and differences, and to determine the extent to which the approved programs reflect the ten content areas of the Mississippi Comprehensive Health Framework. This study also examined the status of secondary school health education programs in Mississippi by utilizing selected data from the U.S. Department of Health and Human Services Centers for Disease …

Mathematical Modeling, Simulation, And Time Series Analysis Of Seasonal Epidemics., Eric Shu Numfor

Electronic Theses and Dissertations

Seasonal and non-seasonal Susceptible-Exposed-Infective-Recovered-Susceptible (SEIRS) models are formulated and analyzed. It is proved that the disease-free steady state of the non-seasonal model is locally asymptotically stable if R_v < 1, and disease invades if R_v > 1. For the seasonal SEIRS model, it is shown that the disease-free periodic solution is locally asymptotically stable when R̅_v < 1, and I(t) is persistent with sustained oscillations when R̅_v > 1. Numerical simulations indicate that the orbit representing I(t) decays when R̅_v < 1 < R_v. The seasonal SEIRS model with routine and pulse vaccination is simulated, and results depict an unsustained decrease …

Discovery And Characterization Of An Antibiotic From The Soil Bacterium Bacillus Sp., Thomas S. Barber

Electronic Theses and Dissertations

Many important antibiotics have become nearly obsolete due to the rise of antibiotic resistant pathogens. Rhodococcus, an actinomycete related to the prolific antibiotic producing genus Streptomyces, harbors over 30 genes for secondary metabolism that could be involved in antibiotic production. Several antibiotics have already been reported for Rhodococcus, suggesting the genus may be a good source for new inhibitory compounds. Fifty four soil bacteria were isolated using enrichment culture techniques (including 37 Rhodococcus) and screened for antibiotic producers. BTHX2, a species of Bacillus was found to have activity against Micrococcus luteus and Rhodococcus erythropolis. BTHX2 …

The Sir Model When S(T) Is A Multi-Exponential Function., Teshome Mogessie Balkew

Electronic Theses and Dissertations

The SIR can be expressed either as a system of nonlinear ordinary differential equations or as a nonlinear Volterra integral equation. In general, neither of these can be solved in closed form. In this thesis, it is shown that if we assume S(t) is a finite multi-exponential, i.e. function of the form S(t) = a+ ∑ⁿ_k=1 r_ke^-σ_kt or a logistic function which is an infinite-multi-exponential, i.e. function of the form S(t) = c + a/b+e^wt, then …

Tobacco-Induced Changes To Porphyromonas Gingivalis Gene Expression, Phenotype And Host-Pathogen Interactions., Juhi Bagaitkar

Electronic Theses and Dissertations

Tobacco smoke is a strong and independent risk factor for several chronic systemic diseases and also increases susceptibility to a multitude of bacterial infections, including periodontal infections. Periodontitis is a chronic inflammatory disease of the supporting tissues of the periodontium caused by its chief etiological agent, Porphyromonas gingivalis . Smokers are more prone to persistent infections by P. gingivalis , and harbor higher numbers of P. gingivalis than non-smokers. However, smokers show reduced clinical signs of inflammation compared to non-smokers, making diagnosis of periodontal disease in smokers problematic. While several studies delineate the different mechanisms of how tobacco smoke alters …

Sa-4-1bbl As A Modulator Of T Effector To T Regulatory Cell Conversion., Shravan Madireddi 1980-

Electronic Theses and Dissertations

The immune system is of profound importance for host survival due to its ability to clear many acquired diseases. In case of infections and cancer, disease arises due to under-performance of immune system. Whereas in autoimmunity and transplantation, deleterious immunity to normal self-antigens and allogeneic antigens arises due to enhanced immune responses. Regulatory T (Treg) cells play a critical role in immune homeostasis in health and disease. Treg cells are important in the development and maintenance of immune tolerance towards self or transplantation antigens. However, Treg cells also play an obstructive role in cancer-related immunity by suppressing various effector cells. …

Assessing Commercial Organic And Conventionally Grown Vegetables By Monitoring Selected Heavy Metals Found In Them., Charles Kafui Dotse

Electronic Theses and Dissertations

Commercially available organic and conventionally grown vegetables were studied by quantitative determination of selected metals in them and to determine if any differences found are statistically significant. These findings can help the consumers to determine if the vegetable products are within the recommended maximum limits as proposed by the joint FAO/WHO Expert committee on organic foods designation. Eight edible vegetables were purchased from local stores in both the organic and conventionally grown categories. Samples were digested with concentrated nitric acid and the metals monitored were zinc, copper, lead, iron, cadmium, and nickel using flame atomic absorption. The concentration range for …

Health Promotion Self-Efficacy Of Belizean Nurses : Variables Influencing Condom Use Promotion To Women To Prevent Hiv/Aids., Whitney A. Nash

Electronic Theses and Dissertations

This dissertation is a descriptive, correlational study that explores how a nurse's personal sexual relationship power, vicarious experience, and other interpersonal and socio-cultural variables are related to her self-efficacy to promote and teach condom use to women to reduce the risk of contracting HIV/AIDS. How these variables are related among a specific subset of nurses with lower sexual relationship power is also examined. Self-efficacy theory, specifically portions of Dr. Albert Bandura's work is used to guide this exploration. Chapter 1 introduces the problem of HIV/AIDS in Belize; the role of power, and selected issues surrounding women and HIV/AIDS. Chapter 1 …

Simulation-Based Metrics Analysis Of An Outpatient Center., Elvin Webb

Electronic Theses and Dissertations

With more and more attention surrounding healthcare, Industrial Engineers have championed the task to help hospitals and outpatient centers operate as efficiently as possible. Simulation is often used to analyze hospital performance measures. The University of Louisville Health Care Outpatient Center is a relatively new building occupying 169,000 ft2 and opened in October of 2008. The clinic is experiencing uneven workloads, over scheduling of Medical Assistants, and highly variable patient waiting times. The Arena Simulation package has been used to develop a model of the outpatient center's current state. Using the data from July 2009 the model has been validated …

The Contextual Impact Of Income Inequality On Social Capital And Adverse Social Outcomes, Jeannie Schiff

Electronic Theses and Dissertations

An interdisciplinary approach to policy and governance recognizes that many social welfare problems are interrelated, and policy-makers have long recognized a need to address the root causes of these problems. There is much evidence that income inequality is one of these root causes but research suggesting the effect of income inequality is mediated by social capital has complicated the relationship, as have theories of causality that take different approaches. This study takes an ecological approach to these issues to test the relationship between income inequality, social capital and selected adverse outcomes proposed by the relative income hypothesis. The relative income …

Social Capital Influences In Women At Risk For Poor Pregnancy Outcomes, Jennifer James-Mesloh

Electronic Theses and Dissertations

Poor pregnancy outcomes such as prematurity, low birth weight and infant mortality are societal indicators of a nation’s health status. These indicators have remained at exceptionally high rates in the United States despite the levels of resources and technology. In the quest to understand that discrepancy, among the ranges of theories and models for explaining poor pregnancy outcomes an emerging concept is coming to attention: social capital. In order to test whether maternal social capital has an impact on pregnancy outcome, women in a Healthy Start program were surveyed over a 13-month period to assess their social capital levels and …

The Impact Of Self-Efficacy, Commitment, And Coping On Occupational Strain In Non-Managerial, Non-Professional Employees, Susan Leslie Bennett

Electronic Theses and Dissertations

The current study explored the effects of moderators, self-efficacy and commitment, and mediators, problem-focused coping (strategies used when changeable conditions exist, thereby resulting in the employee taking action [Folkman & Lazarus, 1980]) and emotion-focused coping (perception that conditions are not changeable and emotions are regulated in a variety of ways versus taking action [Folkman & Lazarus, 1980]), on predicting psychological and physical occupational strain in non-managerial, non-professional employees. Ninety-three shift workers in a 24/7 call center from one division of a transportation company located in the western United States participated in the study. The first research objective was to examine …

Linear Enamel Hypoplasia At Santa Rita Corozal, Belize, Andrew Peter Tetlow

Electronic Theses and Dissertations

The focus of this thesis is an analysis of a sample of dentition collected from the Postclassic Maya site of Santa Rita Corozal in Northern Belize. The goal of this study is to determine what the presence (or absence) of Linear Enamel Hypoplasia (LEH) can demonstrate about the general health (i.e. stress, disease, nutrition, and weaning age) and social status of a single subset of the Late Postclassic (900-1500 CE) Maya living at Santa Rita Corozal. Specifically, this thesis focuses on dentition of thirteen individuals from a large Postclassic platform group. The sample consists of sub-adult and adult female dentition …

Effectiveness Of Probiotics In Preventing Antibiotic Associated Diarrhea And Clostridium Difficile In Long Term Care, Marva Edwards-Marshall

Electronic Theses and Dissertations

Problem/Purpose: Antibiotic associated diarrhea (AAD) and clostridium-difficile diarrhea (CDAD) are the most common forms of infectious diarrhea in long-term care facilities. The purpose of this study was to determine the effectiveness of probiotics in preventing AAD and CDAD in the long term care geriatric population, and to identify interventions that can be used to improve clinical practice. Background/Significance: Prophylactic use of probiotics have been purported to decrease the incidences of AAD and CDAD. Previous studies have yielded contradictory results on the efficacy of probiotics. The objective of this study was to evaluate the impact of administration of probiotics on the …

Identification Of Novel Antimalarials From Marine Natural Products For Lead Discovery, Stephenie M. Alvarado

Electronic Theses and Dissertations

An estimated 500 million cases of malaria occur each year. The increasing prevalence of drug resistant strains of Plasmodium in most malaria endemic areas has significantly reduced the efficacy of current antimalarial drugs for prophylaxis and treatment of this disease. Therefore, discovery of new, inexpensive, and effective drugs are urgently needed to combat this disease. Marine biodiversity is an enormous source of novel chemical entities and has been barely investigated for antimalarial drug discovery. In an effort to discover novel therapeutics for malaria, we studied the antimalarial activities of a unique marine-derived peak fraction library provided by Harbor Branch Oceanographic …

Growing Local: Anthropological Reflections On Current Challenges Facing Central Florida Organic Farmers, Cheney Swedlow

Electronic Theses and Dissertations

This thesis considers Central Florida's emerging local food movement from an anthropological perspective. Area farmers and organizations spearheading this movement and the benefits of purchasing and consuming locally grown food are ethnographically explored. Interviews with natural and organic farmers highlight the challenges affected farmers face in creating a sustainable local food movement in the greater Orlando region. Their motivations for farming organically and the counter-hegemonic tendencies inherent in this mode of cultivating are critically analyzed. Taken as a whole, this work addresses the limitations and opportunities afforded to farmers amid the popularity of local food consumption as a social movement. …

A Single Case Study Of A Three Year Coordinated School Health Program At A Rural North Mississippi Elementary School, Sandra Lena Howell

Electronic Theses and Dissertations

The single case study presents the story of the implementation of a three year Coordinated School Health Program (CSHP) at a rural North Mississippi school. The school was recognized by the Mississippi Department of Education, Office of Healthy Schools, as one of ten selected model Mississippi healthy schools. An awarded grant of one hundred thousand dollars funded the implementation of the CSHP over a three year period. The study examined the impact of the CSHP on the students, staff, and personnel. Two types of data were collected and analyzed including archived documents and interviews conducted with five members of the …