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A Theory-Based Intervention To Mitigate Weight Bias Among Nurses: A Pilot Study, Gregory S. Marler

Electronic Theses and Dissertations

Background: As obesity rates continue to rise, a growing number of individuals living with obesity will seek medical care, increasing their exposure to weight-biased healthcare providers. This bias presents a significant risk, as it can lead to compromised care, diminished trust in medical professionals, and ultimately poorer health outcomes. Nurses, as frontline caregivers, must recognize the harmful impact of weight bias and take proactive steps to mitigate its effects. Addressing this bias is essential to ensuring equitable, high-quality care and improving patient outcomes.

Aim: The study explored the acceptability, feasibility, and preliminary efficacy of an intervention for acute care …

Dirty Dianthus: A Review Of The Microbotryum-Dianthus Plant Pathogen System., Roxanne Kaaren Hayes

Electronic Theses and Dissertations

Microbotryum dianthorum is a species complex of smut fungi that act as biotrophic plant pathogens of Dianthus flowering plants. This group of pathogenic fungi are situated within a larger group of fungi that act as biotrophic plant pathogens, some of which are responsible for extensive crop loss in our agricultural system. These pathogens are distinct from other Microbotryum species in their ability to infect not only a single host, but multiple hosts. Their unique ability to parasitize several plant hosts may unlock potential clues about the potential for emergent infection disease in crop systems. Previous work has established that these …

Healthcare Access And Agency In Material Culture: A Specialized Analysis Of Pharmaceutical Artifacts From Louisville’S West End., Sara Marian (Deurell) Ignacio

Electronic Theses and Dissertations

The American Victorian Period of the 1870s to 1920s was a time of significant transitions in medicine. In Louisville, Kentucky, this period saw an influx of Black healthcare professionals and the founding of Louisville National Medical College, the state’s first medical school open to people of color. A number of these professionals lived and/or practiced in the Beecher Terrace study area, which was excavated between 2017 and 2020. Patent medicine brands and archival records were examined qualitatively in order to understand neighborhood and case study household health concerns and consumer choice. Statistical analyses of the medicine bottles recovered during the …

Sars-Cov-2 Modulates Pla2g2d/Pgd2 Signaling To Manipulate Host Immune Response To Increase Disease Severity., Dante L. Hamiel

Electronic Theses and Dissertations

Background: Previous studies suggested that SARS-CoV-2 infection could cause diverse immune and metabolic dysregulations, although the underlying mechanisms remain poorly understood. In addition, viruses may escape the immune response by modulating cellular metabolism, mitochondria, and protein functions indicating that immune or metabolic components may represent novel targets for anti-viral strategies. However, whether immune and metabolic disorders interact or affect each other waits to be determined. On the other hand, previous investigations identified the critical roles of a cell membrane-bound phospholipase PLA₂G2D and its downstream products PGD₂ in regulating the metabolism and inflammation in respiratory system. More importantly, …

Assessing The Relationship Between The Incidence Of Campylobacteriosis And Ecological Risk Factors In Tennessee, Emmanuel Angmorteh Mensah

Electronic Theses and Dissertations

Background: Campylobacteriosis although is the leading causes of bacterial diarrheal illnesses, have not gained prominent awareness and there are gaps in the understanding of transmission routes for sporadic, non-outbreak cases and their risk factors at the communal level to humans. This study, therefore, aimed to identify the campylobacteriosis risk factors and risk perception from the existing literature in the United States, determine the incidence and ecological risk factors for campylobacteriosis zip code level in Tennessee, and to perform spatiotemporal analysis of campylobacteriosis incidence cases in Tennessee.

Method: To achieve the first aim, a scoping review was conducted to synthesize …

Angelic Wasting: The Impacts Of The Enlightenment, Evangelicalism, And The Industrial Revolution On Romanticization Of Tuberculosis In England, 1800-1850, Sara M. Sudano

Electronic Theses and Dissertations

This thesis aims to elucidate the intersections between the portrayal of tuberculosis in the Romantic era and the Enlightenment, evangelism, and the Industrial Revolution. This study aims to do so by locating and analyzing depictions in the primary source representations of the disease, then considering the effects of the three factors mentioned above on its representation. The Enlightenment, evangelism, and the Industrial Revolution interacted in a variety of ways, and the influence of all three factors was found within the representations of tuberculosis, albeit in often indirect ways. In the novels of Elizabeth Cleghorn Gaskell and Charlotte Brontë, tuberculosis was …

Medical Professionals' Perceptions Of Healthcare Artificial Intelligence, Falon M. Costigan

Electronic Theses and Dissertations

This study examined whether medical professionals across different levels of expertise show differences in their perceptions of healthcare artificial intelligence (AI). Specifically, the researcher hypothesized that participants with more expertise and experience would have greater negative perceptions associated with AI in healthcare compared to participants with a lower expertise level. Participants completed a questionnaire evaluating their perceptions of healthcare AI across four components: long-term negative consequences of AI, need for AI safeguards, positive perceptions of AI, and AI control. Participants also completed demographic questions evaluating participants' age, sex, ethnicity, years working in a healthcare setting, medical …

Synthetic Protein Mimetic Based Therapies For Neurodegeneration, Nicholas H. Stillman

Electronic Theses and Dissertations

Protein-protein interactions (PPIs) are crucial for the regulation of a majority of, if not every fundamental cellular process. Despite their role in regular biological processes, dysregulated and/or aberrant protein-protein interactions (aPPIs) are often related to the onset of disease including cancer, viral infection, and amyloid diseases. aPPIs have historically been deemed ‘undruggable’ due to their large surface area and lack of a binding cavity; however, today, more than 40 disease-related aPPIs have been targeted with small molecules and several of those have reached clinical trials.

A class of synthetic protein mimetics called oligopyridylamides (OPs) has been shown to inhibit disease-related …

A Qualitative Study: The 4 I’S Of Transformational Leadership And The Impact It Has On Building Employee Resiliency, Jewel Hale, Jr.

Electronic Theses and Dissertations

The COVID-19 pandemic has created a turbulent environment for governments and citizens worldwide. In this context, one concept stands out as particularly valuable: resilience. Resilience, the capacity to bounce back from adversity, is a key component in post-pandemic recovery and evolution. This phenomenological study explores the crisis through the experiences of small business owners and leaders in the Dallas/Fort Worth area of Texas during the pandemic's peak. The participants were selected for successfully navigating the pandemic and remaining in business. The study aims to examine the leadership styles used to build employee resilience, focusing on their commonalities through phenomenological research. …

Don't Let Them In: Our Vampire Stories In The French Quarter, Sela Ricketts

Electronic Theses and Dissertations

This thesis is an exploration of vampires in New Orleans. The reason I have chosen to include the word “Our” in the title is because we created the stories that I am studying. We created, perpetuated, changed, and eventually monetized them. Throughout this paper I discuss how the vampire came to New Orleans and how that same vampire shows up in New Orleans folklore. Then I discuss how the vampire made the jump from urban legend to pop culture. In doing that it gained “humanity”. They were still monstrous, but it was enough humanity to acquire regional identity, a southern …

Maximum Likelihood Estimation For Partially Observed Markov Model Using Different Tail Perturbations, Zamzam Qazi

Electronic Theses and Dissertations

This work is about parameter estimation of POMP models using the two-time scale Iterated Filtering algorithms with p-generalized Gaussian perturbations. The algorithms are effective for estimating numerous types of infectious disease models such as COVID-19, influenza or various other diseases. First, we introduce the generalization of Stein’s identity for normal distribution to p-generalized Gaussian distribution, which enables more flexible perturbation with different tail behaviors. Then, we provide proofs and verify their authenticity by applying them in black box optimization as well as optimization of multivariate state space models. Also, we propose a novel weighted average algorithm for maximizing likelihood through …

Deciphering Genetic Contributions To Antibiotic Persistence: Insights From Multi-Gene Knockout Studies, Elise K. Schweer

Electronic Theses and Dissertations

Antibiotic resistance is a critical global health challenge, complicating efforts to treat bacterial infections effectively. Antibiotic persistence plays a significant role in driving resistance. Persisters are a subpopulation of non-dividing bacterial cells that often evade lethal antibiotic treatment. Once antibiotics are removed, these cells "resurrect," resuming growth and may lead to relapsing infections. Notably, the progeny of persister cells exhibit a heightened tendency to acquire mutations or incorporate antibiotic resistance genes, further compounding the challenge of antibiotic resistance. In our recent study, we knocked out seven hypothetical genes and observed a significant reduction in persister levels. Compared to the wild …

Effects Of Essential Oils Alone Or In Combination With Monensin In An In Vitro Model Of Ruminal Acidosis, Jorge L. Bonilla Urbina

Electronic Theses and Dissertations

The objective of this study was to evaluate the effect of essential oils and/or monensin on the concentration of volatile fatty acids (VFA), ammonia, and pH in a ruminal acidosis in vitro model. Red Angus steers (n = 4; BW = 435 ± 9 kg) with ruminal and duodenal cannulas were allocated in a 4 × 4 Latin square design. Treatments were 1) Fed no essential oils (RB) or monensin sodium (MON:CON); 2) Fed RB at a rate of 14 g daily with no Mon (RB+); 3) Fed no RB and fed Mon at 400 mg daily (MON+); 4) Fed …

Prospective Use Of Detection Dogs As A Screening Tool For Mycoplasma Ovipneumoniae In Bighorn Sheep (Ovis Canadensis), Michele C. Lovara

Electronic Theses and Dissertations

Mycoplasma ovipneumoniae is a bacterium that can spill over from domestic sheep (Ovis aries) to bighorn sheep (Ovis canadensis), with effects in bighorn sheep ranging from low to no lamb recruitment to whole-herd all-age die-offs from resulting pneumonia. Fast and reliable pathogen or disease detection is essential for managing outbreaks in bighorn sheep and emergences in affected populations. Detection dogs are increasingly being enlisted to close gaps and further complement various disease, pathogen and virus research and response efforts. This thesis explores the use of conservation detection dogs as a potential tool for M. ovipneumoniae surveillance in bighorn sheep through …

Investigating The Ruminal Metagenome Of Grass And Forage-Fed Bison To Uncover Metabolic Activities That Impact The Efficiency Of Plant Fiber Utilization, Anlly Fresno Rueda

Electronic Theses and Dissertations

This dissertation investigates the ruminal microbiome of American plains bison (Bison bison) to uncover the metabolic activities that enable efficient utilization of plant fiber, with a particular focus on bacterial composition and functional capabilities in response to forage quality. Using both 16S rRNA gene sequencing and metagenomic approaches, this research provides a comprehensive view of microbial diversity and metabolic potential in the rumen of bison, offering insights into their ecological resilience and efficiency in nutrient extraction. Chapter 2 focuses on the metabolic potential of bacterial communities in the rumen of bison fed a grass-based diet. Using high-throughput sequencing and metagenomic …

Characterizing A Free-Roaming Cat Population To Predict Predation Of Threatened Species In An Island Ecosystem, Sarah Gheida

Electronic Theses and Dissertations

No abstract provided.

Interactions Of The Sars-Cov-2 Viral Genome 3’-Untranslated Region With Viral And Host Rnas, Caleb Frye, Mihaela Rita Mihailescu

Electronic Theses and Dissertations

This dissertation focuses on the characterization of RNA-RNA interactions within the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome and with host microRNAs. As the causative agent of coronavirus disease 2019 (COVID-19), SARS-CoV-2 has evolved rapidly since its appearance. This has warranted prompt characterization of the virus particularly of its single stranded RNA (ssRNA) genome. By using a combination of bioinformatics, biophysics, and/or biological assays, we analyzed the SARS-CoV-2 viral genomic RNA and uncovered interactions of genomic RNA with host RNAs, highlighting an underutilized method of targeting RNA viruses. We showed here that the conserved elements in the viral genomic …

Evaluating The Implementation Of Community Schools’ Strategies To Address Post-Pandemic Student Mental Health, Dawn Gordon

Electronic Theses and Dissertations

This research explores the systemic and structural barriers affecting the effectiveness of community schools, particularly in providing mental health services and support, with a specific focus on how principals have responded to these needs in the post-COVID-19 era. Employing qualitative methods, including focus groups with principals from schools implementing the community schools’ model, the study identifies three major themes: difficulties with lead agency collaboration and coordination with host schools, systemic and structural barriers to effective functioning, and holistic approaches to enhancing student support and well-being with mental health.

Difficulties with lead agency collaboration and coordination reveal how inadequate communication and …

Rna Structural-Dynamical Hierarchy: Sars-Cov, Sars-Cov-2, And Delta/Omicron Variant S2m Monomers, Kissing Complexes, And Extended Duplexes, Adam Kensinger

Electronic Theses and Dissertations

The power and promise of ribonucleic acid (RNA) molecules are only beginning to be realized in living organisms and pharmaceutical intervention. Specifically, defining the chemical physics of RNA’s heterogenous structural ensemble and dynamics, which has experienced expansive growth over the last few years, is central to a complete understanding, yet continues to be a significant challenge. A 41-nucleotide hairpin called the stem-loop II motif (s2m), present in the viral genomes of severe acute respiratory syndrome (SARS), SARS-coronavirus-2 (SARS-CoV-2), and the Delta and Omicron variants responsible for the COVID-19 pandemic, is a potential antiviral target due to its high sequence conservation …

The Ethics Of Involuntary Psychiatric Hospitalization: A Comprehensive Analysis Of Policies, Practice, And Ethical Oversight, Mariah Chobany

Electronic Theses and Dissertations

As a specialized field of medicine, psychiatry presents unique ethical challenges and dilemmas distinct from other medical disciplines. A particularly complex aspect of psychiatric care involves the involuntary treatment of patients who may be unable to make informed decisions about their care or participation in research due to the debilitating effects of their psychiatric condition. This presents a significant challenge for providers and researchers, particularly concerning capacity issues and the informed consent process. Despite the intricacies and complexities of ethical dilemmas in psychiatric treatment and research, there is a notable lack of comprehensive guidance in policies and practices on addressing …

Proteolytic Manipulation Of Neutrophil Responses And The Efferocytic Synapse By Porphyromonas Gingivalis., Kelley N. Cooper

Electronic Theses and Dissertations

Porphyromonas gingivalis (Pg) is etiologically associated with chronic periodontitis, a highly prevalent chronic inflammatory disease characterized by the destruction of hard and soft tissues of the periodontium. Pg is adapted to live in the human oral subgingival niche, a hostile environment dominated by excessive numbers of activated neutrophils and antimicrobial mediators. Its survival is contingent upon prompt and effective inactivation of neutrophil effector functions. In this dissertation, I uncover a novel pathogenic mechanism employed by Pg that disarms the antimicrobial capacity of live neutrophils and simultaneously sequesters them within macrophages. Mechanistically, RgpB, a cysteine protease produced by Pg …

Differential Responses Of Human Neutrophils To Legionella Species., Hannah E. Hanford

Electronic Theses and Dissertations

Legionella species are Gram-negative facultative intracellular bacterial pathogens commonly found in freshwater or soil environments where they survive and replicate within their natural protozoan hosts. Humans serve as an accidental host for Legionella species after inhalation of contaminated aerosolized water droplets or soil particles enabling the bacteria to enter the lungs, infect alveolar macrophages, and result in severe pneumonia. Evasion of degradation and proliferation within alveolar macrophages by Legionella species and manifestation of “Legionnaires’ disease” is dependent on the Dot/Icm type IV secretion system (T4SS). The T4SS apparatus is a needle-like channel for translocation of over ~350 effector proteins, depending …

Plasma Resuscitation Attenuates Hemorrhage-Induced Glomerular Barrier Damage And Dysfunction., William Byron Risinger

Electronic Theses and Dissertations

Introduction: Acute renal dysfunction following traumatic injury and hemorrhagic shock carries significant morbidity and mortality. Despite an extensive understanding of renal tubule damage after injury, less is known regarding early alterations to the glomerular filtration barrier following hemorrhagic shock and resuscitation. Previously, our lab and others have demonstrated improved hepatic and intestinal microcirculatory parameters when resuscitation strategies incorporate fresh frozen plasma. In addition to improving end-organ perfusion, plasma reconstitutes the endothelial glycocalyx, an essential regulator of vascular permeability and microcirculatory function. However, the impact of plasma resuscitation on the glomerular glycocalyx and barrier following hemorrhage remains unknown. We hypothesized …

Victims Unknown: An Evaluation Of The Way Louisville, Kentucky Communicated About Aids At The End Of The 20th Century, Alexander M. Vernon

Electronic Theses and Dissertations

This is thesis examines the way that printed news sources reported on AIDS during the height of the epidemic in Louisville, Kentucky. It compares between mainstream and grass-roots sources to work to identify shortcomings in reporting, and how reporting on a public health crisis can be improved upon in the future. It uses an intersectional framework to deconstruct why certain news sources reported on specific demographics of the population, across racialized and gendered lines. Chapter One analyzes the epidemic and then contextualizes the language that was used. Chapter Two focuses on mainstream local newspapers, while also defining demographics of AIDS …

Community College Student Success During Covid-19 Pandemic Based On Class Modality, Rebecca Aslinger

Electronic Theses and Dissertations

The purpose of this non-experimental, quantitative, comparative study was to determine whether there were significant differences in Introduction to Education grades as measured by GPA among community college students between 2018-23 with the change of class modality due to the Covid-19 pandemic.

This study examined differences in Introduction to Education grades as measured by GPA at three community colleges from 2018-23. Archival data were used for this study. The population for this study consisted of all students enrolled in the Introduction to Education course at three institutions. Both in-person and online classes were examined during the time before, during, and …

Self-Rated Interprofessionalism Between Allied Health Professionals, Courtney Andrews

Electronic Theses and Dissertations

Preventable deaths due to medical errors are a significant cause of death in the United States. Decreased communication and collaboration has been cited as a potential reason for these preventable medical errors. Interprofessional practice (IPP) is a service delivery method where medical professionals from different disciplines intentionally work together in order to provide collaborated and collective care for patients. Interprofessional education (IPE) training programs are becoming more common in healthcare education, and have been cited as one way to improve IPP among healthcare providers. However, gaps exist in the literature regarding how to best structure and implement these programs so …

An Exploration Of K-3 Teachers’ Beliefs, Attitudes, And Instructional Technology Use Pre-, During, And Post-Covid-19, Patience Mensah-Bonsu Aggrey

Electronic Theses and Dissertations

This qualitative multiple case study explores kindergarten to third-grade teachers use of instructional technology across three different periods: pre-COVID-19, during COVID-19, and post-COVID-19. This research investigates teachers’ attitudes, the use of technological tools by teachers, and the internal (second order) and external (first order) barriers that informed their use of instructional technology before and during the COVID-19 pandemic and continue to influence their use in the post-pandemic phase. Data were collected through questionnaires, interviews, and checklists from eight kindergarten third-grade teachers using a qualitative multiple case study approach. The findings suggest that there was a shift in teachers’ instructional technology …

College Student’S Learning Experiences During Covid-19 Among Traditional And Post-Traditional Students Enrolled In Institutions Affiliated With The Appalachian College Association, Jo Anne Smith

Electronic Theses and Dissertations

The purpose of this study was to determine the differences in the learning experiences, feelings and emotions, level of study support, and online learning during the COVID-19 pandemic between traditional and post-traditional college students enrolled in an institution affiliated with the Appalachian College Association.

A two-group comparison research design using a web-based survey was used for the study. Using the College Student’s Learning Experiences during COVID-19 among Traditional and Post-Traditional Students Enrolled in Institutions Affiliated with the Appalachian College Association survey, the research questions were analyzed with a sample size of N = 58, traditional college student group and N=33, …

Overcoming The Barrier To The Clinical Application Of Naloxone: Application Of Drug Delivery Technologies, Akeemat O. Tijani

Electronic Theses and Dissertations

Opioid overdose crisis continues to demand innovative solutions for rapid and effective drug administration. Naloxone (NAL), a critical opioid antagonist, has been the primary treatment for reversing opioid-induced respiratory depression. However, its current delivery methods, such as intravenous and intranasal formulations, are limited by their invasive nature, short half-life, and the need for repeated dosing. This dissertation investigates advanced drug delivery technologies aimed at overcoming these challenges and enhancing the clinical utility of NAL.

This research focused on improving the transdermal and transnasal delivery of NAL using minimally invasive techniques. Various approaches were evaluated, including sonophoresis, microneedles, laser ablation, dermaplaning …

Service Provider Perceptions Of Systems Serving Individuals Experiencing Homelessness: A Needs Assessment And Strategic Plan In Johnson City, Tn, Opal Frye-Clark

Electronic Theses and Dissertations

This Integrated Learning Experience (ILE) investigates service providers' perceptions of Johnson City, Tennessee's homelessness services, aiming to inform a city-wide strategic plan for reduction. Employing a mixed-methods approach, the study gathered qualitative data through in-depth interviews with service providers in 2022 and quantitative data via a 2024 survey. The research focused on those directly involved in serving or significantly influencing the lives of individuals experiencing homelessness (IEH). Three key aims guided the investigation: (1) To analyze provider perspectives to identify community assets, opportunities, barriers, and unmet needs, informing a comprehensive needs assessment; (2) To refine these findings through SWOT analysis …