Digital Commons Network^™

Exosomes From Embryonic Stem Cells As A Prophylactic Vaccine Against Lung Cancer., Shuhan Meng

Electronic Theses and Dissertations

The antigenic similarity between embryos and tumors has raised the idea of using embryonic materials as a preventative vaccine against neoplastic disease. Indeed, a previous study reported that a vaccine comprised of allogeneic murine embryonic stem cells (ESCs) and murine fibroblasts expressing immune-stimulatory granulocyte macrophage-colony stimulating factor (GM-CSF) successfully blocked the outgrowth of an implantable lung cancer (Lewis lung carcinoma; LLC) and lung tumors caused by a combination of a mutagen followed by chronic pulmonary inflammation. However, such a vaccine is obviously impractical for application to humans. The use of fibroblasts to produce GM-CSF is needlessly complicated, and intact live …

Development Of In Vitro Models To Study The Rapid Extraintestinal Dissemination Of Salmonella., Adarsh Gopinath

Electronic Theses and Dissertations

Salmonella appears in the bloodstream of mice in as little as 15 minutes after oral inoculation and establishes persistent colonies in the spleen and liver. While its pathway to blood is undetermined, this phenomenon is dependent on the activity of Salmonella pathogenicity island 2 (SPI-2) coded type III secretion system (T3SS) and CD18+ phagocytes. We hypothesize that dendritic cells associated with the basal face of the gut epithelium, that are naturally migratory and known to sample for luminal antigens directly transport Salmonella to the bloodstream. This process comprises of at least two phases, dissociation and reverse transmigration. We define dissociation …

Efficient Conditional Inactivation Of Rdh10 Reveals Important Role Of Retinoic Acid During Lung Branching Morphogenesis., Nhut Quang Huy Tran

Electronic Theses and Dissertations

Birth defects are complicated pathological processes. Many birth defects can be traced back to disrupted regulation of molecular signaling pathways during embryogenesis. One of the most important gene regulatory factors during embryo development is retinoic acid (RA). However, studying the specific roles of RA in each organ during embryogenesis in vivo is challenging. The Sandell laboratory has a conditional mutant mouse model that can potentially be used for studying the role of RA during embryogenesis by inducing stage-specific RA deficiency. This model allows retinol dehydrogenase 10 (Rdh10), a gene required for RA production, to be inactivated at a …

The Impact Of State-Level Laws On Syringe Service Program Access And Risk Environment Of People Who Inject Drugs (Pwid), Samuel Pettyjohn

Electronic Theses and Dissertations

Background: Understanding concentrated areas with high rates of opioid use disorder (OUD) allows for improved placement of Narcan access points through syringe services programs (SSPs). People Who Inject Drugs (PWID) have lower risk of contracting infectious diseases the closer they are to SSPs. Tennessee law prohibits SSPs within 2000ft of a school or park, impacting the placement of SSPs in non-urban areas. Testing factors related to SSP siting placement within a system dynamic model can better determine the relationship between PWID risk environment and SSP access and utility.

Methods: We identified areas of greatest need for harm reduction interventions within …

Blend It Like Beckett: Samuel Beckett And Experimental Contemporary Creative Writing, Sam Nicole Campbell

Electronic Theses and Dissertations

Samuel Beckett penned novels, short stories, poetry, stage plays, radio plays, and scripts—and he did each in a way that blended genre, challenged the norms of creative writing, and surprised audiences around the globe. His experimental approach to creative writing included the use of absurdism, genre-hybridization, and ergodicism, which led to Beckett fundamentally changing the approach to creative writing. His aesthetics have trickled down through the years and can be seen in contemporary works, including Aimee Bender’s short story collection The Girl in the Flammable Skirt and Mark Z. Danielewski’s novel House of Leaves[1]. By examining these works …

Chronic Pain And History Of Trauma, Lauren K. Grizzard

Electronic Theses and Dissertations

As literature has shown, chronic pain patients present with a unique challenge to the medical field. The existing question is if the patient’s pain is unmanageable because of the severity of the pain or if the patient lacks coping mechanisms. If there is a lack of coping mechanisms, then that raises a plethora of new questions for the medical teams caring for them. After IRB approval, the current study was conducted on a sample of three patients that were referred to the Hendrick Medical Center Pathways team for pain management. It sought to determine if there was any association between …

The Effect Of Care Coordination On Hemoglobin A1c For Type 2 Diabetes Patients, Lee Ann Hinsky

Electronic Theses and Dissertations

The purpose of the study was to test the impact of care coordinator visits by primary care registered nurses on serum hemoglobin A1c (HbA1c) levels in patients with type 2 diabetes. In the study, the researcher compared 2 groups of type 2 diabetes patients—those who had care coordinator visits from registered nurses and those who did not—through retrospective data collection and analysis from the patients’ electronic medical record from July 2018 through December 2018. An independent samples t test was used to compare the means of the intervention and control groups’ change in HbA1c levels. The intervention and control groups …

The Eu Ets: A Critical Analysis, Marceline Grey Swan

Electronic Theses and Dissertations

Carbon markets, established via cap & trade programs, are the dominant means by which climate stabilization is currently being pursued worldwide, with many centered around the European Union’s Emissions Trading Scheme. As such, a critical analysis of the EU ETS & its effectiveness is crucial to assess whether the EU carbon market is an effective mechanism to achieve climate stabilization. The effectiveness of the overall program was evaluated via a determination of whether the EU ETS, & to a greater extent the EU carbon market, has allowed for emissions reductions in line with the goal for climate stabilization set by …

Development Towards An Ethylene Sensing Artificial Metalloenzyme, Mitchell Lee Ellinwood

Electronic Theses and Dissertations

Ethylene (ethene) is the essential ripening hormone for climacteric plants, and consequently its detection and quantification is of considerable interest to the horticulture industry. Its detection however is a non-trivial pursuit, traditionally requiring expensive and inaccessible instrumentation that fail to provide in vivo information about ethylene. A ruthenium based fluorescent probe for the selective detection of ethylene has recently been developed, and it was envisioned that this probe could be made biocompatible by encasing it within a protein scaffold making it an artificial metalloenzyme. To this end biotinylated N-heterocyclic carbenes (NHCs) were synthesized, which can be appended onto the ruthenium …

Social Distance, Stigma, And Help-Seeking: An Exploration Of Autism Beliefs In The Southern United States, Christian Shannon Courson

Electronic Theses and Dissertations

Stigma has been associated with physical and mental health conditions and their treatment long before formal care systems. Negative perceptions within the lay community, such as stigma and social distancing, negatively impact individuals with autism and their families. The purpose of this study was to explore factors associated with autism stigma among laypeople in the Southern United States. Specifically, the study explored how demographics, knowledge, and familiarity are related to social distancing and stigma, and how these factors relate to beliefs about and intentions of help-seeking. Using a lay sample (N=108) of rural Southern towns, survey data was collected in …

Physiological Responses To Feeding A Yeast Culture Supplement To Transition Dairy Cows, Nathaly Ana Carpinelli

Electronic Theses and Dissertations

The transition period is the dynamic stage in the lactation cycle, where the most metabolic changes can occur. The use of nutritional strategies, such as yeast culture, can help cows during the transition from pregnancy to early lactation, improving health and decreasing the incidence of metabolic disorders. The first objective of this research was to evaluate the effects of a commercial yeast culture product (YC; Cellerate Culture Classic HD, Phibro Animal Health, Teaneck, NJ) on performance, blood biomarkers, and rumen fermentation and bacterial population in dairy cows during the transition period through 50 days in milk (DIM). Overall, the results …

Antidiabetic Activity Of Cissus Rotundifolia Plant Growing In Saudi Arabia, Saad Ali Alshehri

Electronic Theses and Dissertations

Diabetes mellitus (DM) is a metabolic disease characterized by high levels of blood glucose resulting from defects in insulin production or action. A world-wide increase in the diabetes rate is projected to reach pandemic levels over the next 10-20 years. Current data shows that high numbers of the population in Saudi Arabia suffer from diabetes and prediabetes. Non-traditional treatments of diabetes from medicinal plants has shown potential antidiabetic activity. A considerable percentage of the population still prefer or use medicinal plants as a treatment or supplement to traditional medicine. Cissus rotundifolia (family Vitaceae) is a known plant in southwestern Saudi …

Habitat Selection Across A Temperature And Nutrient Gradient By Cope’S Grey Treefrog (Hyla Chrysoscelis) And The Mosquito, Culex Restuans, Sarah Christine Mcnamara

Electronic Theses and Dissertations

Habitat selection by pond breeding amphibians and insects is often critical for larval survival, performance, and resulting parental fitness, since there is typically little parental care beyond oviposition site choice. Numerous axes of patch quality have been identified and quantified, but species with varied life histories may differently shift perception of habitat quality as changes in global climate increase water temperatures and rates of eutrophication. Three outdoor mesocosm experiments were conducted using two species with complex life cycles and very different life histories; Hyla chrysoscelis, or Cope’s Gray tree frog, and the mosquito (Culex restuans). Two mesocosm experiments were conducted …

Transgenerational Effects Of Mycoplasma Gallisepticum Infection In Eastern Bluebirds (Sialia Sialis), Sarah Danielle Amonett

Electronic Theses and Dissertations

Parents can transmit pathogens to their offspring. Neonatal hosts are particularly vulnerable to infectious diseases, as they have immature adaptive immune systems at birth. In this study, I examine parental transmission of a combacterial pathogen Mycoplasma gallisepticum (MG) in wild eastern bluebirds (Sialia sialis) and its impact on nestling growth. By diagnosing infections in all family members, I confirmed that eastern bluebird nestlings can contract MG infections, and that infection was more comin older nestlings compared to adults and neonates. Furthermore, unlike in other avian hosts, MG infection does not significantly impact nestling body condition. In response to infection, eastern …

Identification And Genomic Characterization Of Candidate Starch And Lactate Utilizing Bacteria From The Rumen Of Beef Cattle, Venkata Bandarupalli

Electronic Theses and Dissertations

The rumen functions as an anaerobic fermentation chamber where microbial communities ferment feedstuffs into SCFAs. These SCFAs are critical to the ruminant host since they fulfill 70% of its energy requirements. In intensive management systems, ruminants are fed with grain-rich diets, which contain high levels of starch that help meet the energy demands of high producing animals. When grain-based diets are fed to ruminants, ruminal amylolytic and lactate metabolizing bacteria predominate. Grain overload can cause rapid production and accumulation of lactate, resulting in proliferation of amylolytic bacteria and death of lactate metabolizing bacteria. Consequently, high lactate levels result in a …

Transcription Regulation Of Human Il1b Gene In Monocytes And Lymphoid Cd4 T Cells, Sree H. Pulugulla

Electronic Theses and Dissertations

Cytokines are key regulators of the inflammatory response and play an important role in facilitating intercellular communication between various immune cell types. Interleukin‑1β (IL‑1β) is a potent pro-inflammatory cytokine that is required for robust initiation of innate immune response and subsequent development of adaptive immunity. IL-1β is first synthesized as an inactive cytoplasmic, non‑glycosylated, precursor molecule (proIL‑1β) by monocytes and macrophages in response to invading pathogenic microbes. The activation of caspase‑1 by inflammasomes cleaves proIL-1β into mature biologically active IL-1β that is released from cells via a non-classical, endoplasmic reticulum‑independent secretory pathway directly from the cytoplasm via Gasdermin D membrane …

Electrospun Fibers And Nanoparticles For The Prevention Of Sexually Transmitted Infections., Kevin Michael Tyo

Electronic Theses and Dissertations

Human immunodeficiency virus-1 (HIV-1) and herpes simplex virus 2 (HSV-2) affect hundreds of millions of people worldwide, with women disproportionately impacted by these infections. Currently, only oral pre-exposure prophylaxis (PrEP) is approved specifically for the prevention of HIV-1, but is challenged with adverse side effects associated with long-term use. Topical delivery platforms, such as gels and films, deliver agents directly to the female reproductive tract, but are limited in providing transient-release. The technology of polymeric electrospun fibers may serve as alternative topical delivery platform to the female reproductive tract. In these studies, we fabricated electrospun fibers comprised of different polymers …

Addressing The Problem Of Sterile Drug Injecting Supply Acquisition For People Who Inject Drugs., Barbra Ann Cave

Electronic Theses and Dissertations

This dissertation contains five chapters, including three manuscripts, covering harm reduction and some of the challenges people who inject drugs (PWID) face in their effort to reduce the risk of injection drug-related harms through sterile injecting supply acquisition and use. Chapter One discusses some background related to PWID and provides an overview of the issues addressed in this dissertation. Chapter Two includes a literature review covering the harm reduction concept, important aspects of syringe exchange programs as a harm reduction tool, and the social determinants of health model as a potential tool for future research. Chapter Three is a two-phase …

The Genesis And Development Of Deathscapes In America -- A Story Of How Chicago And Louisville Cemeteries Demonstrate The Shifting Rationale Of Cemetery Placement During The 19th And 20th Centuries., Thomas D. Cleven

Electronic Theses and Dissertations

Today, most construction projects require a systematic site qualification based on a suitability analysis utilizing parameters such as slope, soil type, elevation, distance to open water, and distance to transportation. The proper siting determines the success of a project in terms of project stability and longevity. However, has this suitability analysis exist for one of the most significant phases of humanity – death. Historically dead bodies seem to have been placed without suitable qualification being many cemeteries have created environmental problems for the living. Hence, with which placement rationale has been used comes to mind. With a varied array of …

Role Of Topoisomerase Ii Alpha In Dna Topology And T Cell Responses During Chronic Viral Infections, Stella Chinyere Ogbu

Electronic Theses and Dissertations

The clearance of viruses is largely dependent upon the activation of T cells to generate a robust immune response. However, host responses are suppressed during chronic viral infections. In this thesis, we explored the role of Top2α in DNA topology in individuals with chronic HBV, HCV, and HIV infections. We found that Top2α protein expression and activity were low in T cells derived from chronically virus-infected individuals compared to healthy subjects. Using CD4⁺ T cells treated with Top2α inhibitor or poisoner as a model, we demonstrated that Top2α inhibition disrupts the DNA topology, suppresses DNA repair kinase (ATM), and …

Examining Opportunities To Increase Flu Vaccine Uptake On College Campuses Through Pharmacy Partnerships, Kristi Hargrave

Electronic Theses and Dissertations

The purpose of this project was to examine a university health clinic’s influenza vaccination program in a suburban city in a western U.S. state, and to explore the effects of partnering with a local pharmacy to overcome billing issues. A program evaluation was conducted to identify the potential barrier of cost for students. The health clinic was not set up to bill private health insurance for students; therefore, if students wanted to get a flu shot, they had to pay out of pocket for it. A pilot program was created in conjunction with the program director, partnering with the local …

Evaluating The Effects Of Sanitizing Drinker Lines In Commercial Broiler Houses On The Water Quality And Microbial Content In Biofilm Layers, Kennedi Achilles

Electronic Theses and Dissertations

The importance of water quality and its ability to cause major impacts, whether it is positive or negative, is often overlooked in the poultry industry. The microbial content of water is just one of many water characteristics that need to be monitored and regulated to ensure a flock is receiving good quality water. A field study was conducted to determine the effects of four different sanitizers under commercial broiler house conditions, with three replications over a three flock period. Each flock was grown for 56 days with a 2 week period between each flock. Four oxidative sanitizers were used and …

Heat Waves And Heat-Related Mortality In East Tennessee, Taiwo Adesoba

Electronic Theses and Dissertations

Heat waves represent a public health challenge that requires multiple responses and warnings to protect vulnerable populations. Although studies have reported an increasing trend of heat wave occurrence in many areas of the world, no clear trend exists in East Tennessee. Using data from Parameter-elevated Relationships on Independent Slope Models (PRISM), CDC WONDER and the United States Census Bureau, the relationship between mortality rates and year was estimated during heat wave events between 1999 and 2010. Five heat wave definitions were tested. Overall, 2007 and 2010 stand out as the years with the highest number of heat wave days in …

The Distinct Expressions Of Integrins Αdβ2 And Αmβ2 Differently Regulate Macrophage Migration In 3d Matrix In Vitro And In Tissue During Inflammation, Kui Cui

Electronic Theses and Dissertations

Chronic inflammation is an essential mechanism during the development of cardiovascular and metabolic diseases. The outcome of diseases depends on the balance between the migration and accumulation of macrophages in damaged tissues. Macrophage motility is highly regulated by adhesive receptors, integrins. Namely, intermediate expression of integrin supports macrophage migration, while a high integrin density inhibits it. Our studies are focused on evaluation of the contribution of related integrins α_Dβ₂ and α_Mβ₂ to macrophage migration and development of chronic inflammation.

We found that integrin α_Dβ₂ is upregulated on M1-macrophages in vitro and …

Arachidin 3 Modulation Of Lipid Metabolism In Rotavirus Infections, Stormey Wisdom

Electronic Theses and Dissertations

Rotavirus (RV) can cause severe and deadly gastroenteritis in young children, infants, and immunocompromised individuals. Previous studies have shown that arachidin 3 (A3) inhibits RV replication, and that RV replication is dependent on the presence of lipids. This study investigated the alteration of lipid metabolism by A3 in RV infected HT29.f8 cells. A decrease in the RV regulation of lipid biosynthesis genes was observed with the addition of A3 using qRT-PCR. Also, immunofluorescent and histochemical staining for neutral fats, a major component of cellular lipid droplets, revealed an increased accumulation with both RV and RV+A3 when compared to no virus …

The Efficacy Of An Educational Intervention To Improve Low Influenza Vaccination Rates Among Federal Health Care Workers, Cynthia Berfect-Shelby

Electronic Theses and Dissertations

Influenza is a preventable communicable illness that has a significant impact on people of all ages. In 2018 it was estimated that 80,000 people died of influenza-related illnesses. Infants and elderly people are among the most vulnerable populations. The Centers for Disease Control and Prevention and Healthy People 2020 recommend that health care facilities have a 90% vaccination rate. Despite the recommendations and a mandatory directive, many facilities within the Veterans Health Administration struggle to reach influenza vaccination rates above 60%. Pender’s health promotion model was used as the theoretical framework to explore the reasons why health care workers refuse …

Public Health Policy: An Ethical Analysis Of Quarantine, Dina Alqahtani

Electronic Theses and Dissertations

As a public health measure quarantine has both historical and contemporary significance both in the United States and abroad. On the surface it represents a low-cost, low-tech way in which the spread of disease can be mitigated as its core requirement is that those who may have been exposed to an infectious agent are kept away from those who have not been exposed to that agent for enough time to determine whether or not infection has been spread. This has been utilized for centuries with both limited questions and scattered, inconsistent, or impossible to achieve oversight and goals. In understanding …

Precision Medicine And It's Ethical And Social Implications: Public Health And Gobal Persepctives, Evangel Sarwar

Electronic Theses and Dissertations

Ever since President Obama's launch of the Precision Medicine Initiative (PMI) in 2015, precision medicine (PM) has been anticipated as the new paradigm for healthcare with the capacity to “empower patients, researchers, and providers to work together toward the development of individualized care,” through research, technologies, and policies (President Obama, 2015). Precision Medicine (PM), in the form of genomics, offers unprecedented promise of providing new tools for improving health and reducing the burden of diseases, not just for the U.S. - but also globally. According to World Health Organization, genomics research and precision medicine will play a major part in …

Experimental Immunology And The Potential For Osteopathological Reconstructions : Pursuing An Experimental Foundation For The Skeletal Inflammatory Index., Megan Elizabeth Duncanson

Electronic Theses and Dissertations

Individuals who mount a strong inflammatory response may produce a shift in the systemic levels of inflammatory mediators, which may lead to a potential hyper-inflammatory phenotype (HIP). Systemic inflammation can increase severity in local inflammatory processes detected in bone lesions. This thesis investigated in vitro if human immune cells pre-treated with inflammatory inducers would affect the inflammatory response against Porphyromonas gingivalis or Staphylococcus aureus (both associated with osteological lesions). We exposed human peripheral blood mononuclear cells to bacterial lysates, or pro-inflammatory cytokines. Sequentially, we exposed the same culture to either P. gingivalis or S. aureus. The final expression of …

Vlogging Truth To Power : A Qualitative Study Of Resilience As Practiced By Transgender Youtube Content Creators., Corey J. Feger

Electronic Theses and Dissertations

This thesis presents an exploratory account of ways that transgender people’s personal YouTube channels, or “vlogs,” provide new avenues to cultivate resilience as a collective. To make sense of this unstable, contested model of identity and community, I apply a three-part model of “social resilience,” a theory of resilience that transcends the individual and welcomes incoherency, contradiction, and “messiness” into its analysis. In Chapter One, I provide a snapshot of transgender history and present my research objective and justification. Chapter Two consists of a literature review and argues in favor of a hybrid theory of intersectionality and assemblage. Chapter Three …