Exploring Foundations Of Animal Host-Microbe Interactions In A Freshwater Sponge, Jacob S. Pawley

Electronic Theses and Dissertations

The most ancient eukaryotic immune defense mechanisms evolved in unicellular species. Multicellular eukaryotes, however, expanded these mechanisms to meet challenges unique to multicellular life, including barrier formation, long-distance cell signaling, and coordinated responses that eliminate microbial threats while minimizing damage to the host. As such, animal immune signaling pathways are exceptionally intricate and diverse. Though, most of what is understood about animal host–microbe interactions stems from a small handful of model systems. Here, I evaluate host–microbe interactions and innate immunity in sponges using the highly tractable freshwater sponge, Ephydatia muelleri. I report the discovery of an external host-derived matrix …

Health Professions Students’ Perceptions Of Medical Laboratory Scientists: Professional Practice And Scope Of Practice, Thomas W. Williamson

Electronic Theses and Dissertations

Medical Laboratory Scientists (MLSs) are vital yet often overlooked members of the healthcare team. This study investigated perceptions of MLSs among students in medicine, pharmacy, and nursing programs. Grounded in professional identity theory and literature on interprofessional stereotypes, the study utilized a quantitative survey distributed to 1,113 students, with 71 responses analyzed. Nine research questions explored differences in perceptions of MLSs’ professional practice, scope of practice, entry requirements, value as team members, and consultative roles. Results indicate students generally value MLSs as members of the healthcare team and acknowledge their contributions to patient care; however, their understanding is frequently restricted …

Isolation And Self-Efficacy Among Texas Educators With Disabilities Post-Covid: A Narrative Inquiry, Paul M. Nimon

Electronic Theses and Dissertations

The purpose of this qualitative inquiry was to explore the narratives of 10 current Texas public school educators with disabilities, as defined by the Americans with Disabilities Act, to understand how these narratives shaped their perceptions of burnout in relation to isolation and self-efficacy in the aftermath of the COVID-19 pandemic. The specific problem addressed the systemic, cultural, and interpersonal barriers that contribute to emotional exhaustion and professional marginalization for this population. The paradigm used was action research. The specific research methodology was a qualitative narrative inquiry. Data collection methods included 60-to-90-minute semistructured interviews conducted via Zoom and transcribed for …

Beyond Cough Drops And Band-Aids: An Interpretive Phenomenological Study Of Certified School Nurses’ Professional Scope And Enacted Roles In Pk–12 Settings, Brandy Gershon

Electronic Theses and Dissertations

This qualitative dissertation explored the underutilization of certified school nurses (CSNs) in PK–12 educational settings across urban, suburban, and rural school districts in western Pennsylvania. Despite their advanced preparation and wide-ranging scope of practice, school nurses are often limited to providing episodic care, including first aid, medication administration, and treatment of minor injuries. This limited use reflects a significant misalignment with their professional scope of practice, which encompasses chronic disease management, health education, mental health support, care coordination, and leadership in public health. Guided by role theory (Biddle, 1986), this study examined how school nurses perceived and interpreted their professional …

Empowering Women Journalists’ Preparedness: An Evaluation Of Hostile Environment First Aid Training (Hefat) And Its Implementation In Academic Journalism Programs, Vanessa Charlot

Electronic Theses and Dissertations

This Dissertation in Practice examines the potential integration of gender-sensitive Hostile Environment and First Aid Training (HEFAT) into journalism education to enhance the preparedness of women journalists for high-risk reporting contexts. Manuscript I investigates existing gaps in curricula, highlighting the distinct physical, psychological, and ethical risks encountered by women in hostile environments. Manuscript II outlines a framework for qualitatively evaluating an adapted HEFAT program, considering how identity-informed and trauma-sensitive approaches might strengthen student safety, resilience, and ethical decision-making. Manuscript III explores leadership strategies for implementing inclusive, trauma-informed HEFAT curricula, emphasizing reflexive practice and self-authorship as mechanisms for fostering transformative, equity-driven …

Health And Care: Childhood Among The Classic Period Maya At Buenavista Del Cayo, Belize, Lindsey Hall

Electronic Theses and Dissertations

Three deposits of perinates, infants, and young children within two non-residential superstructures of Buenavista del Cayo, Belize, display evidence of daily maintenance and care. A total of ten subadult individuals, aged 39.4 weeks gestation to 3 years, from the archaeological site of Buenavista were recovered during 2018 excavations. Signs of care and daily maintenance include survivorship through the vulnerable neonatal period (first twenty-eight days) and transition into infancy, survivorship with potential pinprick lesions on external and internal zygomatic, parietal, and pars basilaris, and intentional maintenance of the body after death. Identification of care and daily maintenance at Buenavista informs the …

Examining The Relationship Between Co-Curricular Programming And Burnout Among Medical Students At An Academic Medical Center, Lauren Williams

Electronic Theses and Dissertations

This three-manuscript dissertation examines the relationship between co-curricular programming and burnout among Doctor of Medicine (MD) students at the University of Mississippi Medical Center (UMMC), situating the issue within broader concerns about physician well-being and institutional responsibility.

Manuscript I establishes the conceptual and empirical foundation for the study by reviewing the prevalence, consequences, and theoretical framing of burnout in medical education. Drawing on Maslach’s three-dimensional model of burnout—emotional exhaustion, depersonalization, and reduced personal accomplishment—and Astin’s Student Involvement Theory, the manuscript situates co-curricular engagement as a potential protective factor against distress. The literature review highlights alarming rates of depression, suicidal ideation, …

Development And Evaluation Of Ros-Responsive Thioketal-Capped Mesoporous Silica Nanoparticles For Dual Drug Delivery Of Moxifloxacin And Curcumin In Wound Healing, Saiganesh Pothuraju

Electronic Theses and Dissertations

Chronic infected wounds remain a major clinical challenge because elevated reactive oxygen species (ROS) in the wound microenvironment sustain inflammation, damage regenerating tissue, and limit the effectiveness of conventional antimicrobial and anti-inflammatory therapy. To address this problem, this study aimed to develop ROS-responsive thioketal-capped mesoporous silica nanoparticles (TK-MSNs) for the dual delivery of moxifloxacin (MOX) and curcumin (CUR) to support the management of infected wounds. Mesoporous silica nanoparticles (MSNs) were synthesised using tetraethyl orthosilicate (TEOS) and cetyltrimethylammonium bromide (CTAB), followed by template removal, amino-functionalization with 3-aminopropyltriethoxysilane (APTES), and sequential loading of MOX and CUR. ROS-responsive capping was then achieved using …

Open For Victims, Sorry We're Dead, Sela Ricketts

Electronic Theses and Dissertations

This thesis is a documentary exploration of paranormal tourism in the French Quarter neighborhood of New Orleans. As of completing this project, paranormal tourism is relatively new to the city, starting in earnest in the late 1990s and early 2000s. This niche industry has woven itself into the larger New Orleans tourism industry and drastically changed the landscape and popular identity of the French Quarter. The photos give a personal perspective of how the industry manifested within the city.

The throughline of this project is a feedback loop I identified- a cycle of influences that has ultimately made the French …

Stochastic Universal Differential Equations For Epidemiological Modeling: Uncertainty Quantification In Disease Transmission Dynamics, Alice Menaya Armah-Bonney

Electronic Theses and Dissertations

Epidemic forecasting requires not only predictions of expected case counts, but also quantification of uncertainty, although existing surrogate modeling frameworks for agent-based models remain fundamentally deterministic. In this thesis a Stochastic Universal Differential Equation framework is presented that extends the deterministic Universal Differential Equation approach by incorporating a learnable stochastic diffusion term, enabling calibrated probabilistic forecasts while preserving the mechanistic interpretability and computational efficiency of the deterministic baseline. In doing so, a two-phase training algorithm is introduced to ensure stable convergence and the framework is validated against the ensemble output from ExaEpi, an exascale agent-based model of a COVID-19 outbreak …

The Perceived Barriers Of A One-To-One Technology Implementation: A Post-Covid Case Study, Adam Howard Creasy

Electronic Theses and Dissertations

This qualitative dominant, exploratory sequential case study aimed to identify stakeholder perceptions of technology within a one-to-one technology initiative and outline any possible barriers. The study examined the perceptions of teachers, parents, and administrators within a rural K-12 public school district in Pennsylvania. The study provided school district stakeholders with data they can use to improve existing and future initiatives by early intervention within areas of concern identified by the study. Data were collected using a two round approach which includes focus groups and targeted surveys utilizing research-backed electronic survey tools. The research questions addressed in the study were the …

A Systematic Review: Comparative Analysis Of Healthcare Waste Management Practices In Kenya And In The United States, Esther Nyokabi Gathungu

Electronic Theses and Dissertations

This comparative study used a systematic literature review to explore waste management practices in Kenya and the USA, examining predefined concepts to identify new patterns relevant to sustainable healthcare waste management (HCWM) practices. The study aimed to understand sustainable practices in healthcare waste management and how developing nations such as Kenya can benchmark from practices in developed nations such as the USA. While the study identified challenges in waste management across Kenya and the USA, it also identified sustainable practices that could be adopted across contexts. The study followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines …

Butting Heads With Respiratory Disease: Post-Epizootic Demography And Movement-Driven Spillover Risk In A Badland’S Bighorn Sheep (Ovis Canadensis) Meta-Population, Sean P. Ryder

Electronic Theses and Dissertations

Respiratory disease caused by Mycoplasma ovipneumoniae is among the most significant threats to bighorn sheep (Ovis canadensis) conservation in North America. In July 2021, contact between bighorn sheep rams and domestic sheep north of Badlands National Park (BADL), South Dakota, USA introduced M. ovipneumoniae into the Badlands bighorn sheep meta-population, triggering an epizootic that reduced the population by approximately 80%. I evaluated the post-outbreak status of this population through two complementary studies conducted from 2023 to 2025. In Chapter 1, I quantified disease prevalence, adult survival, lamb recruitment, and population growth rate (λ) using serial quantitative polymerase chain reaction (qPCR) …

Mind The Gap: Intergenerational Perspectives On Mental Illness Within Ghanaian American Communities, Ama Danso-Ayesu

Electronic Theses and Dissertations

This research explores attitudes toward mental illness among Ghanaian immigrants and

first-generation Ghanaian Americans, with particular focus on factors that influence

stigma. Guided by Bronfenbrenner’s Bioecological Model, the study utilized the

Community Attitudes Toward Mental Illness (CAMI) Scale to measure four dimensions

of stigma, including authoritarianism, benevolence, social restrictiveness, and community

mental health ideology. A comparative, cross-sectional design was employed to examine

differences between immigrant and American-born participants. The findings revealed

generational differences where Ghanaian immigrants reported higher levels of

authoritarian and socially restrictive beliefs, while first-generation Ghanaian Americans

demonstrated more benevolent and inclusive attitudes. Interpreted through the ecological

systems …

Identifying Correlates Of Protection Of A Pneumonic Plague Vaccine., Caleb Samuel Whitley

Electronic Theses and Dissertations

Pneumonic plague is caused by inhalation of the bacterial pathogen Yersinia pestis. The immune response during Pneumonic plague occurs in two phases; an initial asymptomatic phase followed by a highly inflammatory and destructive phase resulting in 100% mortality if left untreated. The rapid onset of severe disease following the asymptomatic phase makes it difficult to administer antibiotics in time to prevent lethal outcomes. Therefore, the development of an effective vaccine is likely needed to combat pneumonic plague in areas of the world where Y. pestis remains endemic. One candidate subunit vaccine is protective in mice, a natural host for …

Tumor Infiltrating Lymphocytes: From Discovery To Clinical Application., Lewis Clayton Chew

Electronic Theses and Dissertations

This thesis explores the evolution of TIL therapy from its discovery to clinical application, focusing on its use in treating metastatic cancer. Adoptive cell therapy (ACT), particularly TIL therapy, has emerged as a promising alternative to traditional treatments like chemotherapy. This review article traces the historical development of TILs, highlighting key experiments and clinical trials that have shaped its understanding and application as a therapeutic strategy for patients with metastatic and difficult to treat cancers including melanoma. Importantly, this article highlights recent advancements, including the FDA approval of Lifileucel, and explores emerging strategies to enhance therapeutic efficacy of TILs through …

Reclaiming Bodily Autonomy Through Gullah Geechee Natural Healing, Tamaray Yvonne White

Electronic Theses and Dissertations

In today’s sociopolitical climate, restrictive reproductive laws continue to disproportionately target Black women, reinforcing historic patterns of racialized and gendered oppression. This thesis argues that Black women will recommence their usage of traditional herbal healing as both a survival strategy and an act of reclaiming bodily autonomy in defiance of hostile legal, financial, and medical systems. The Gullah Geechee community serves as a blueprint for this transition, offering cultural continuity, intergenerational knowledge, and a model for community-based healthcare and resistance. By tracing the historical necessity of herbal medicine among Gullah Geechee women, this study situates ancestral natural healing within contemporary …

How Fundraisers Met Goals During The Covid-19 Pandemic, Cody C. Greene

Electronic Theses and Dissertations

The COVID-19 pandemic disrupted nearly every aspect of higher education operations, including fundraising and donor engagement. This qualitative study explored how Vice Presidents for Advancement and other senior fundraising leaders in higher education adapted their practices to meet fundraising goals during the pandemic. Using a narrative design, in-depth interviews were conducted with nine participants representing diverse institutions in the southeastern United States. Braun and Clarke’s (2006) six-phase thematic analysis was used to code and interpret the data.

Seven major themes emerged: communications, technology, donor relations, managing a fundraising team, overall giving impact, successful strategies, and the future. Participants described how …

Avaren-Fc's Effects On Lymphoma Cells., Jacob Hahn

Electronic Theses and Dissertations

This thesis investigates the therapeutic potential of Avaren-Fc (AvFc), a novel lectin fusion protein that integrates the Fc region of human IgG1 with the engineered lectin Avaren, specifically targeting lymphoma. The study delineates the in vitro binding capabilities of Avaren-Fc with a human B-cell lymphoma cell line, a canine B-cell lymphoma cell line, healthy canine peripheral blood mononuclear cells (PBMCs), and healthy human PBMCs. AvFc was shown to exhibit significant binding to human lymphoma cells. Furthermore, the direct cytotoxicity of Avaren-Fc against canine lymphoma cells, canine lymphoma cells and healthy canine PBMCs was assessed. It was found that there is …

Enzymatic Depletion Of Adenosine To Potentiate Cancer Immunotherapy., Omar Sadi Sarkar

Electronic Theses and Dissertations

Immune checkpoint blockade (ICB) therapies have revolutionized cancer treatment, but many patients still do not respond well. To improve therapeutic outcomes, chemotherapy is often combined with immunotherapy, as chemotherapy is known to promote immunogenicity. However, many patients develop resistance to this combination therapy, and the reasons behind it are not fully understood. In this study, we explored how platinum-based chemotherapy, specifically cisplatin, affects tumor signaling to create an immunosuppressive tumor microenvironment (TME). We found cisplatin triggers ataxia telangiectasia mutated (ATM)-mediated DNA damage signaling and induces higher expression of prostaglandin E₂ (PGE₂) via a cyclooxygenase-2 (COX-2) mediated pathway. We speculate DNA …

An Investigation Of Parental Involvement In The Safe Passage Initiative (An Anti-Violence Program), Danielle King

Electronic Theses and Dissertations

This dissertation investigates the issue of parental involvement in Safe Passage, an anti-violence initiative operating within seven Pittsburgh Public Schools. Recognizing the involvement of parents is critical for successful violence prevention, this study explores the barriers hindering meaningful participation and identifies strategies to enhance parent-school-community collaboration.

Utilizing qualitative research methods, the study captures insights from parents, educators, and community stakeholders through a reflective Journey Line activity and focus group discussion. Data collected revealed several significant barriers such as inconsistent communication, limited parental availability due to demanding work schedules, mental and emotional exhaustion, cultural disconnects, and a lack of clarity regarding …

Describing Latent Tuberculosis Infection And Treatment Completion In Virginia: A Social Determinants Of Health Approach, Amber N. Harmon

Electronic Theses and Dissertations

While the treatment of latent tuberculosis infection is a key strategy in tuberculosis elimination nationally and globally, there is a limited understanding of the general prevalence of latent tuberculosis as well as factors related to successful treatment. This dissertation describes the known landscape of latent tuberculosis in Virginia and explores possible factors related to latent tuberculosis infection treatment through three manuscripts. The first manuscript, Chapter 1, outlines the overall proposal for this dissertation research in the style of a National Institute of Health grant proposal. The second manuscript, Chapter 2, describes the current understanding of the factors related to latent …

Cbl Regulation Of C-Met Signaling In Corneal Epithelial Cells., Kate Elise Tarvestad Laise

Electronic Theses and Dissertations

The cornea is the most anterior portion of the eye and permits light passage through to retina, allowing formation of a clear image as long as it remains healthy. If the cornea sustains damage, it creates and entrance for foreign body invasion, infection, and ultimately blindness in the immunocompromised eye. Healing the corneal tissue is critical for vision restoration and pain alleviation, as it is the most densely innervated tissue in the body. Growth factors and their cognate receptors are currently under investigation as tools to restore proper corneal physiology. We hypothesize that manipulating the hepatocyte growth factor (HGF) /c-Met …

Reentry For Formerly Incarcerated Individuals: A Focus On Workforce Reentry And Recidivism In Appalachia, Margaret E. Francisco

Electronic Theses and Dissertations

Formerly incarcerated individuals face challenges to successful reentry, a critical component of reducing recidivism and improving public health outcomes. These challenges are particularly acute in rural Appalachia, where structural disadvantages such as limited economic opportunities, healthcare access, and public transportation intersect with stigma and systemic barriers related to prior incarceration. This dissertation addresses these gaps through a mixed-methods examination of reentry and recidivism, grounded in public health frameworks and focused on the unique context of rural Appalachia.

This work includes three studies. First, a PRISMA-guided systematic review synthesized qualitative research on barriers and facilitators to employment following incarceration. Findings highlighted …

Administrator Perceptions Of Title I Parent And Family Engagement Practices And Policies During Covid-19, Rebekah Owens

Electronic Theses and Dissertations

The purpose of this study was to examine Title I administrator perceptions of facilitating parent and family engagement practices and policies prior to, during, and post Covid-19. The literature shows one of the leading factors in a child’s academic learning is family engagement (Henderson & Mapp, 2002). The Dual Capacity-Building Framework for Family-School Partnerships highlights ways in which administrators and school staff can be successful in engaging families and building strong partnerships by meeting four different capacity skills. Data collection strategies included semi-structured, one-on-one interviews, via Zoom video or audio interview, observation, document review, and field notes. Analysis of data …

Orchestrated Architecture Dismantling: Borrelia Burgdorferi’S Coordinated Destruction Of Adaptive Immune Responses, Craig A. Land

Electronic Theses and Dissertations

Borrelia burgdorferi represents the most prevalent vector-borne bacteria in North America, affecting ~476,000 Americans annually yet persisting despite robust immune responses. This project reveals spirochetes target adaptive immunity through coordinated lymphatic colonization and precision immune dysregulation.

Using comparative host models (C57BL/6 vs. reservoir Peromyscus leucopus), we demonstrate lymph node colonization within 48-hours, with spirochetes exploiting LYVE-1-positive lymphatic structures as dissemination highways while avoiding the interior of follicular regions. Critically, we identify targeted CD4+ T-cell elimination in germinal center border zones at 21-DPI, precisely when these cells should be coordinating protective immunity. Concurrent upregulation of inhibitory receptor CD32b creates molecular …

Teachers’ Perceptions Of The Work Environment And Job Satisfaction Before And After Covid-19, Carla Sexton

Electronic Theses and Dissertations

This phenomenological, qualitative study examined teachers’ perceptions of the work environment and job satisfaction before and after COVID-19. The findings may support school leaders by providing insight into what keeps teachers fulfilled in their careers, aiding in the retention of teachers in the district. Purposeful sampling was used to identify general education teachers who taught in one school district before and after COVID-19. Twelve interviews were conducted using open-ended questions and semi-structured questions to gain a better understanding of teachers’ experiences and perceptions. Each interview was transcribed and coded by the researcher. The results of the interviews led to the …

Institutional Betrayal And Military Vaccine Mandates: An Inquiry Into Loss Of Health Autonomy Concerning Unlimited Liability Contractual Employment In The Covid-19 Era, Aaron G. Dehaven

Electronic Theses and Dissertations

The balance between government control and personal liberty is a growing concern in the modern world. Nowhere is this more evident than in discussions surrounding government policy in public health and individual health autonomy. The Covid-19 pandemic forced many institutions to change their standard operating procedures by adapting to rapidly changing health guidance instituted to protect public health. These changes highlighted the role of health autonomy and individuals’ rights to refuse medical treatments. The Department of Defense changed many aspects of how it operated during the pandemic, including the mandate of the Covid-19 vaccine for all service members. For various …

Political Ecologies Of The Livestock Industry, Jecca Bowen

Electronic Theses and Dissertations

In the United States today, livestock production is dominated by an intensified, industrialized production paradigm whose growth in recent decades has utterly transformed the logistical realities and community impacts of animal agriculture. This dissertation investigates the effects of this production paradigm on livestock producers—whose decision-making is shaped by multi-scalar and often contradictory forces—as well as residents living near these large operations. To do so, I draw upon mixed-methods fieldwork, including in-depth interviews, spatial analysis, and field odor measurements in Weld and Morgan Counties in northern Colorado. I also use political ecologies of emotion, embodiment, and health to examine the complex …

Alterations And Improvements Of The Photoacoustic Flow Cytometry Method, Nicholas J. Taylor

Electronic Theses and Dissertations

The emergence and proliferation of antibiotic-resistant bacteria present a significant and growing challenge to global public health, with the potential to render many existing antibiotics ineffective. Infections caused by these resistant pathogens are difficult to treat, resulting in higher morbidity, mortality, and healthcare costs. A major obstacle in combating these infections is the slow pace of traditional diagnostic methods, such as blood cultures, which typically take 2-3 days to identify the causative bacteria and determine their antibiotic resistance profiles. This delay in diagnosis hinders timely interventions and appropriate treatment, further contributing to the spread of resistant infections. As such, there …