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A Study Of Gpr3, Gpr6, Of Gpr12 As Novel Molecular Targets For Cannabidiol., Alyssa S. Laun May 2018

A Study Of Gpr3, Gpr6, Of Gpr12 As Novel Molecular Targets For Cannabidiol., Alyssa S. Laun

Electronic Theses and Dissertations

The G protein-coupled receptors 3, 6, and 12 (GPR3, GPR6, and GPR12) comprise a family of closely related orphan receptors with no confirmed endogenous ligands. These receptors are constitutively active, capable of signaling through G protein and non-G protein mediated mechanisms. These orphan receptors have previously been reported to play important roles in many normal physiological functions and to be involved in a variety of pathological conditions. Although they are orphans, GPR3, GPR6, and GPR12 are phylogenetically most closely related to the cannabinoid receptors. Using β-arrestin2 recruitment and cAMP accumulation assays, we have recently found that the non-psychoactive phytocannabinoid cannabidiol …

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African American Grandmothers As The Black Matriarch : You Don't Live For Yourself., Tanisha Nicole Stanford May 2018

African American Grandmothers As The Black Matriarch : You Don't Live For Yourself., Tanisha Nicole Stanford

Electronic Theses and Dissertations

This study examined historical and contemporary roles of African American grandmothers within the familial system, and their socio-psychological experiences. The primary method of data collection was semi-structured, conversational style interviews with an oral history aspect. There were six grandmothers interviewed, two from the midwest region of the United States, and four from the southern region. The findings reveal stories that corroborate with the literature on the role of women in African American families and that of the Black matriarch, considering their strength are not inherent but necessary. They are not born matriarchs or strong black women, they become that person …

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The Effects Of Polychlorinated Biphenyls Exposure On Non-Alcoholic Fatty Liver Disease : Role Of Aryl Hydrocarbon Receptor., Hongxue Shi May 2018

The Effects Of Polychlorinated Biphenyls Exposure On Non-Alcoholic Fatty Liver Disease : Role Of Aryl Hydrocarbon Receptor., Hongxue Shi

Electronic Theses and Dissertations

Polychlorinated biphenyls (PCBs) are detectable in serum of 100% of adults in US, and has been associated with fatty liver disease in epidemiological studies. PCBs are classified as either dioxin-like (DL) or non-dioxin-like (NDL) PCB based on their ability to activate the aryl hydrocarbon receptor (AhR). We used exposures that reflect human bioaccumulation patterns, which resembles Aroclor 1260 with a low level of the DL-PCB, PCB 126. Our aim was to determine if this exposure will activate the human and mouse AhR and examine if receptor activation influences these steatotic responses due to PCB exposures. DL-PCBs exposure-induced AhR activation in …

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Inflammasome Independent Leukotriene-B4 Production Drives Crystalline Silica Induced Sterile Inflammation., Bindu Hegde May 2018

Inflammasome Independent Leukotriene-B4 Production Drives Crystalline Silica Induced Sterile Inflammation., Bindu Hegde

Electronic Theses and Dissertations

Silicosis is an irreversible lung inflammatory disease caused by chronic exposure to crystalline silica (CS) and is one of the most prevalent occupational diseases worldwide. Lipid chemoattractant Leukotriene B4 (LTB4) plays an important role in neutrophilic inflammation that drives silicosis and promotes lung cancer. Previous studies in our laboratory have demonstrated that CS-induced neutrophilic inflammation and lung tumor burden in K-rasLA1 mice is abrogated in the LTB4-receptor deficient mice. Another pathway whose importance is well studied in the progression of silicosis is the Nalp3 inflammasome pathway. Studies have shown inflammasome- dependent IL-1b to be …

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There Is Water In The World For Us : The Environmental Theories Of Alice Walker., Janae Lewis Hall May 2018

There Is Water In The World For Us : The Environmental Theories Of Alice Walker., Janae Lewis Hall

Electronic Theses and Dissertations

The emergence of African-American Environmental thought responds to the ongoing erasure of Black experiences and their perspectives on nature. Mainstream environmentalism maintains a legacy of perceived innocence and incorruptibility towards the land, while Black Environmentalism demonstrates the limitations of that ideology. Limitations include the erasure of history in regards to stealing land from Indigenous people, the brutality of slavery, legalized lynching, forced removal from the land, exploitation in sharecropping, destruction of sacred lands, heavy pollution in urban centers, and harmful environmental policies. For Black and Indigenous peoples, it is impossible to view American soil as innocent. This project surveyed the …

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State-Reinforced Self-Governance Of Community-Managed Open Spaces In Chicago, Il And Louisville, Ky., Willow Sequoia Dietsch May 2018

State-Reinforced Self-Governance Of Community-Managed Open Spaces In Chicago, Il And Louisville, Ky., Willow Sequoia Dietsch

Electronic Theses and Dissertations

As urban populations rise, small greenspaces, like gardens, are increasingly important to well-being of communities, and urban sustainability as a whole. However, past development, and current political and economic challenges encumber many cities in providing adequate greenspace. Cities like Chicago, IL and Louisville, KY have turned to the communities to manage greenspaces with help from partner organizations. This thesis examines these arrangements, and compares them in terms of several potential factors, (i.e. legal authority, responsibility, and support). Semi-structured interviews of important community greenspace stakeholders, and archival sources including original documents, news articles, and government reports, were used to understand the …

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Vinyl Chloride Enhances Diet-Induced Liver Injury Via Metabolic Dyshomeostasis : Critical Role Of Mitochondria., Anna L. Lang May 2018

Vinyl Chloride Enhances Diet-Induced Liver Injury Via Metabolic Dyshomeostasis : Critical Role Of Mitochondria., Anna L. Lang

Electronic Theses and Dissertations

Background. Vinyl chloride (VC) is an environmental toxicant and has been shown to be directly hepatotoxic at high exposures. However, recent studies suggest low-level toxicant exposure can cause subtle changes to the liver. Given the high prevalence of non-alcoholic fatty liver disease (NAFLD) in the United States, it is important to determine the impact of low-level toxicant exposure on the progression of underlying liver injury when combined with other factors. Therefore, the overarching goal of this dissertation was to develop a model of VC co-exposure with high-fat diet (HFD) and to determine the mechanisms by which VC contributes to the …

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Nutritional Virulence Of Legionella Pneumophila., Ashley M. Best May 2018

Nutritional Virulence Of Legionella Pneumophila., Ashley M. Best

Electronic Theses and Dissertations

Legionella pneumophila is an environment organism that parasitizes a wide range of protozoa. Growth within the environmental host primes L. pneumophila for infection of human alveolar macrophages when contaminated aerosols are inhaled. Intracellular replication within either host requires the establishment a replicative niche, known as the Legionella-containing vacuole (LCV). Biogenesis of the LCV depends on the type IVb translocation system, the Dot/Icm, to translocation >320 effectors into the host cytosol. Effectors are responsible for preventing lysosome fusion to the LCV, recruitment of ER-derived vesicles to the LCV, and modulation of a plethora of host processes to promote the intracellular …

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Gender Boundary Negotiation Within The U.S. Immigrant/Refugee Resettlement : How Transnational Bridge-Building Matters., Telesphore Kagaba May 2018

Gender Boundary Negotiation Within The U.S. Immigrant/Refugee Resettlement : How Transnational Bridge-Building Matters., Telesphore Kagaba

Electronic Theses and Dissertations

For the past several decades, social research about U.S. immigrant and refugee resettlement has focused on some of the ways federal and non-governmental organizations help immigrants and refugees integrate into mainstream society. Little research, if any, has explored how giving voice to immigrants and refugees is done in bridging transnational gaps and strengths in the process of their adjustment to the host country. This dissertation is grounded within transnational theory (Avenarius 2012, Levitt 2011, Peteet 1997) that argues that immigrants and refugees bring their experiences and cultures from their home countries, time in refugee camps, and other often traumatic experiences …

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Functional And Structural Impact Of The Loss Of The Leucine-Rich Repeat Protein Lrit1 In The Mouse Retina., Catherine Ann Cobb May 2018

Functional And Structural Impact Of The Loss Of The Leucine-Rich Repeat Protein Lrit1 In The Mouse Retina., Catherine Ann Cobb

Electronic Theses and Dissertations

Mutations in genes encoding the leucine-rich repeat (LRR) proteins nyctalopin and LRIT3 lead to complete congenital stationary night blindness because they are critical to depolarizing bipolar cell function in the retina. LRIT3 has two closely related family members, LRIT1 and LRIT2. In silico analyses of publicly available RNA-Seq data showed that Lrit1 was highly expressed in the retina. Here I describe the expression pattern and impact of loss of LRIT1 on retinal function. To enable these studies, we used CRISPR/Cas9 technology to create an Lrit1-/- mouse line. Retinal morphology and morphometry analyses showed no gross changes in retinal structure …

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The Styles Of Criminology : Writing Among The Stars., Ethan Maxwell Higgins May 2018

The Styles Of Criminology : Writing Among The Stars., Ethan Maxwell Higgins

Electronic Theses and Dissertations

This study investigates how criminological writing style is "a [more] complicated terrain" than we realize in criminology (Butler, 1990, p. xix). Data is drawn from interviews with forty of the one hundred most influential criminological writers (Walters, 2015) to explore perceptions of good writing in the field, namely in describing writing practices, norms and values. Thus, the findings of this study come in three chapters. The first two result chapters explore the terrain of writing in criminology by presenting perceptions of best practices and values of good writing. The third results chapter then examines these perceptions within a lens of …

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Gender And Leadership In Animal Sheltering Organizations., Jennifer Blevins Sinski May 2018

Gender And Leadership In Animal Sheltering Organizations., Jennifer Blevins Sinski

Electronic Theses and Dissertations

This study explores the connection between gender, leadership and implementation of best practice strategies to reduce or eliminate euthanasia of healthy, adoptable companion animals in animal sheltering organizations. The purpose of this mixed methods study utilizing an online survey and in-depth interviews is to explore the impact of gender and animal sheltering leadership on organizational policies, as well as workers’, and volunteers’ attitudes toward reducing euthanasia in sheltering organizations. It is anticipated that the knowledge generated from this research will provide new insights into animal sheltering policies and procedures and inform sheltering organizations about the connection between gendered leadership in …

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Assessment And Optimization Of Environmental Systems Using Data Analysis And Simulation., Milad Ebrahimi May 2018

Assessment And Optimization Of Environmental Systems Using Data Analysis And Simulation., Milad Ebrahimi

Electronic Theses and Dissertations

For most environmental systems, specifically wastewater treatment plants and aquifers, a significant number of performance data variables are attained on a time series basis. Due to the interconnectedness of the variables, it is often difficult to assess over-arching trends and quantify temporal operational performance. The objective of this research study was to provide an effective means for comprehensive temporal evaluation of environmental systems. The proposed methodology used several multivariate data analyses and statistical techniques to present an assessment framework for the water quality monitoring programs as well as optimization of treatment plants and aquifer systems. The developed procedure considered the …

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A Qualitative Case Study Examination Of Athlete Ally Using Social Movement Theory., Samuel H. Schmidt May 2018

A Qualitative Case Study Examination Of Athlete Ally Using Social Movement Theory., Samuel H. Schmidt

Electronic Theses and Dissertations

The following study answered the call for more social movement related studies in sport (Davis-Delano & Crosset, 2008) and lessened the paucity of literature on athlete activism. The purpose of this study was to examine a sport specific social movement organization, Athlete Ally, using Social Movement Theory. Social Movement Theory is guided by three tenets: Resource Mobilization Theory (Edwards & McCarthy, 2004), Political Process Theory (Kriesi, 2004), and Cultural Theory/Frame Analysis (Williams, 2004). Each of these tenets was used to examine the resources exchanged between Athlete Ally and their athlete constituents, determine the strategies for engaging with various sport (i.e. …

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Longitudinal Tracking Of Physiological State With Electromyographic Signals., Robert Warren Stallard May 2018

Longitudinal Tracking Of Physiological State With Electromyographic Signals., Robert Warren Stallard

Electronic Theses and Dissertations

Electrophysiological measurements have been used in recent history to classify instantaneous physiological configurations, e.g., hand gestures. This work investigates the feasibility of working with changes in physiological configurations over time (i.e., longitudinally) using a variety of algorithms from the machine learning domain. We demonstrate a high degree of classification accuracy for a binary classification problem derived from electromyography measurements before and after a 35-day bedrest. The problem difficulty is increased with a more dynamic experiment testing for changes in astronaut sensorimotor performance by taking electromyography and force plate measurements before, during, and after a jump from a small platform. A …

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A Framework For Cardio-Pulmonary Resuscitation (Cpr) Scene Retrieval From Medical Simulation Videos Based On Object And Activity Detection., Anju Panicker Madhusoodhanan Sathik May 2018

A Framework For Cardio-Pulmonary Resuscitation (Cpr) Scene Retrieval From Medical Simulation Videos Based On Object And Activity Detection., Anju Panicker Madhusoodhanan Sathik

Electronic Theses and Dissertations

In this thesis, we propose a framework to detect and retrieve CPR activity scenes from medical simulation videos. Medical simulation is a modern training method for medical students, where an emergency patient condition is simulated on human-like mannequins and the students act upon. These simulation sessions are recorded by the physician, for later debriefing. With the increasing number of simulation videos, automatic detection and retrieval of specific scenes became necessary. The proposed framework for CPR scene retrieval, would eliminate the conventional approach of using shot detection and frame segmentation techniques. Firstly, our work explores the application of Histogram of Oriented …

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Divergent Evolution Of Di-Lysine Er Retention Vs. Farnesylation Motif-Mediated Anchoring Of The Ankb Virulence Effector., John David Perpich May 2018

Divergent Evolution Of Di-Lysine Er Retention Vs. Farnesylation Motif-Mediated Anchoring Of The Ankb Virulence Effector., John David Perpich

Electronic Theses and Dissertations

Legionella pneumophila is an aquatic organism capable of intracellular replication within a wide range of protozoan hosts and within human macrophages where it causes Legionnaires’ Disease. L. pneumophila manipulates a variety of host cell processes by translocating, into the host cell cytosol, more than 300 effector proteins via the type IVB secretion system (T4SS). The AnkB effector from the AA100/130b strain (AnkB-AA100) contains a C-terminal CaaX motif that rapidly becomes farnesylated upon translocation into the host cell and anchored to the cytosolic face of the Legionella-containing vacuole (LCV) membrane, which is essential for intracellular replication. We show that a …

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A New Surgical Approach For O-C2 Fusion, Solving The Problem Of Dysphagia., M. Kathryn Mcclure May 2018

A New Surgical Approach For O-C2 Fusion, Solving The Problem Of Dysphagia., M. Kathryn Mcclure

Electronic Theses and Dissertations

The fusion of the second cervical vertebrae to the occipital bone (O-C2 fusion) for head stabilization can result in postoperative dysphagia and dyspnea, negatively impacting the patient’s quality of life. Currently, the O-C2 angle is used for head placement, which may not place the head neutrally. We hypothesize that aligning the external auditory meatus with midline of the C2 will reduce oropharyngeal stenosis, reducing dysphagia. One male patient with poor swallow quality of life who required a revision of a previous O-C2 surgery was evaluated via videofluoroscopy and completed the standard swallow quality of life questionnaire (SWAL-QOL) before and after …

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Designing Interactive Virtual Environments With Feedback In Health Applications., Yi Li May 2018

Designing Interactive Virtual Environments With Feedback In Health Applications., Yi Li

Electronic Theses and Dissertations

One of the most important factors to influence user experience in human-computer interaction is the user emotional reaction. Interactive environments including serious games that are responsive to user emotions improve their effectiveness and user satisfactions. Testing and training for user emotional competence is meaningful in healthcare field, which has motivated us to analyze immersive affective games using emotional feedbacks. In this dissertation, a systematic model of designing interactive environment is presented, which consists of three essential modules: affect modeling, affect recognition, and affect control. In order to collect data for analysis and construct these modules, a series of experiments were …

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Swallow And Breathing Coordination Following Suprahyoid Muscle Injury., Bradley Kimbel May 2018

Swallow And Breathing Coordination Following Suprahyoid Muscle Injury., Bradley Kimbel

Electronic Theses and Dissertations

Swallowing motility disorders (dysphagia) are a major complication following radiation treatment for head and neck cancer, affecting ~50% of those treated. One reason for this is that radiation causes muscle damage, provoking sensorimotor pathologies. Previous work has suggested that injury may cause discoordination between breathing and swallowing behaviors. We sought to determine if muscle injury provokes changes in this behavior. We hypothesized that acute suprahyoid muscle damage would alter cross-behavior excitability, causing destabilization of the respiratory-swallow pattern. Swallowing was evoked in anesthetized spontaneously breathing cats via injection of a 3cc bolus of water into the oropharyngeal cavity. A suprahyoid injury …

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The Effects Of A Physical Activity Program Called "Minds-In-Motion-The Maze" On Balance And Motor Skills In Middle School Aged Students., Liliana Vargas May 2018

The Effects Of A Physical Activity Program Called "Minds-In-Motion-The Maze" On Balance And Motor Skills In Middle School Aged Students., Liliana Vargas

Electronic Theses and Dissertations

Competing time demands in the curriculum have left physical education as a low priority among schools. Consequently, school aged children are not meeting the recommended 150 mins/wk of physical activity. We studied the effects of Minds in Motion-MAZE on middle school aged (n=74) children’s balance and motor skills. Minds in Motion-MAZE is a low cost physical activity program designed to improve balance and motor control. Participants participated in Minds in Motion-MAZE for 15 minutes during the 7th period. A multivariate test did not show a statistical significance of participating in Minds in Motion-MAZE and improved balance and motor skills. …

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Factors Influencing The Retention Of Women Faculty In Stem Disciplines., Carrie J. Christensen May 2018

Factors Influencing The Retention Of Women Faculty In Stem Disciplines., Carrie J. Christensen

Electronic Theses and Dissertations

Low numbers of women faculty in STEM (science, technology, engineering and mathematics) disciplines continues to be a concern in higher education. Even though completion of STEM degrees by women has increased in many disciplines, increases in the number of women faculty have not been seen. Additionally, women continue to leave faculty positions at twice the rate of men. In order to remain globally competitive, the US needs to retain a diverse STEM professoriate. This dissertation examined the factors influencing the retention of women faculty in STEM disciplines and their over-representation in non-research intensive institutions. The analysis was broken into two …

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Traumatic Brain Injury : Investigating Misconceptions Among Graduate Students In Physical Therapy, Occupational Therapy, And Speech-Language Pathology., Michael Frazure May 2018

Traumatic Brain Injury : Investigating Misconceptions Among Graduate Students In Physical Therapy, Occupational Therapy, And Speech-Language Pathology., Michael Frazure

Electronic Theses and Dissertations

This study utilized a convenience sample (n = 510) to investigate misconceptions of traumatic brain injury (TBI) among first and second year graduate students in physical therapy (PT), occupational therapy (OT), and speech-language pathology (SLP) training programs. Eighty-six-point-seven percent of participants were female, and 87.70% were white. All participants completed a survey comprised of items relating to general information about TBI, coma and unconsciousness, memory loss, recovery, and concussion. Descriptive and summary statistics indicated the persistence of misconceptions regarding coma and unconsciousness, memory loss, recovery, and concussion among graduate students in PT, OT, and SLP training programs. Group comparisons …

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Autism Spectrum Disorder : Investigating Predictive Diagnostic Relationships In Children Three Years-Of-Age And Younger., Kaitlin Juergensen May 2018

Autism Spectrum Disorder : Investigating Predictive Diagnostic Relationships In Children Three Years-Of-Age And Younger., Kaitlin Juergensen

Electronic Theses and Dissertations

Autism spectrum disorder (ASD) is a heterogeneous neurodevelopmental disorder whose symptoms may involve deficits across three domains: communication, socialization, and atypical behaviors or interests. With a high prevalence across populations and a tendency to impact males more so than females, early and accurate diagnosis appears critical. The most current literature on ASD provides a myriad of difficulties associated with diagnosis under the age of three years. The purpose of this study was to determine if a predictive relationship exists between a child’s individual developmental domain standard deviation (SD) subscale scores (motor, language, cognitive, social-emotional, and adaptive skills) on the Bayley …

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Institutional Processes To Determine Community Engagement Impact : A Collective Case Study., Amanda Marie Bowers May 2018

Institutional Processes To Determine Community Engagement Impact : A Collective Case Study., Amanda Marie Bowers

Electronic Theses and Dissertations

This qualitative, collective case study is designed to examine the processes by which urban, metropolitan institutions determine the impact their community engagement has within the local community. The study addresses the lack of research on community engagement at the institutional level, the processes that track and coordinate engagement, as well as the perspectives of community partners in this work. Research is more developed regarding individual engagement activities and student learning outcomes than it is to institutional accountability structures or community impact. Studies that center the institution as the unit of analysis are needed to address these limitations in research and …

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Gestures Of Creative Recovery For The Egocentric Actor Through Performance In Wertenbaker's Our Country's Good., Ross Joel Shenker May 2018

Gestures Of Creative Recovery For The Egocentric Actor Through Performance In Wertenbaker's Our Country's Good., Ross Joel Shenker

Electronic Theses and Dissertations

This document serves as a travelogue for the creation of two roles for the U of L Fall 2017 Production of Timberlake Wertenbaker’s Our Country’s Good. At odds throughout the process were intellect and vulnerability. In an attempt to find openness with my primary scene partner, I tried a variety of techniques including, but not limited to: Konstantin Stanislavski’s “Bits and Tasks”; Michael Chekhov’s “Psychological Gesture”; Carl Jung’s theories on Archetype; F.M. Alexander’s notions of “Inhibition” and “Nondoing” as expounded upon by Betsy Polatin; and Julia Cameron’s “Morning Pages.” My original goal of achieving vulnerability on stage became consumed …

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An Agent Based Model To Assess Crew Temporal Variability During U.S. Navy Shipboard Operations, Kevin Muhs May 2018

An Agent Based Model To Assess Crew Temporal Variability During U.S. Navy Shipboard Operations, Kevin Muhs

Electronic Theses and Dissertations

Understanding the factors that affect human performance variability as well as their temporal impacts is an essential element in fully integrating and designing complex, adaptive environments. This understanding is particularly necessary for high stakes, time-critical routines such as those performed during nuclear reactor, air traffic control, and military operations. Over the last three decades significant efforts have emerged to demonstrate and apply a host of techniques to include Discrete Event Simulation, Bayesian Belief Networks, Neural Networks, and a multitude of existing software applications to provide relevant assessments of human task performance and temporal variability. The objective of this research was …

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Sharing The Pain In Social Media: A Content Analysis Of #Thinspiration Images On Instagram, Agustina Amon May 2018

Sharing The Pain In Social Media: A Content Analysis Of #Thinspiration Images On Instagram, Agustina Amon

Electronic Theses and Dissertations

#Thinspiration is an online trend that depicts thin-ideal media content specifically found on the social medium Instagram. The images found under the #thinspiration intend to inspire weight loss while encouraging and/or glorifying dangerous behaviors that are usually attributed to eating disorders including anorexia nervosa (AN), bulimia nervosa (BN) and eating disorder not otherwise specified (EDNOS). This study provides a content analysis of thinspiration imagery on the popular social networking site Instagram. A set of 300 randomly selected images was coded. Images tended to objectify women and sexualize them with a focus on bony and extremely thin women. Results seemed to …

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Tissue Engineered Micro And Macrovasculature Utilizing Stromal Vascular Fraction., Joseph Samir Zakhari May 2018

Tissue Engineered Micro And Macrovasculature Utilizing Stromal Vascular Fraction., Joseph Samir Zakhari

Electronic Theses and Dissertations

This dissertation describes the use of stromal vascular fraction to tissue engineer 3D microvasculature and macrovasculature. Stromal vascular fraction is an easily isolatable cell source from adipose tissue depots. It has demonstrated remarkable potential both in vitro and in vivo for forming microcirculation capable of perfusion upon implantation. SVF is clinically utilized as a therapeutic cell source for anti-inflammation for osteoarthritis and is being studied for ischemic tissue application to stimulate revascularization. The work described herein is divided within four chapters. Chapter I provides an introductory overview and lists the aims and hypothesis for the dissertation. Chapter II describes experiments …

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Optimizing High-Speed Exercise Performance : Effect Of Load., Ling Bai May 2018

Optimizing High-Speed Exercise Performance : Effect Of Load., Ling Bai

Electronic Theses and Dissertations

The purpose of this study was to identify an optimal load for the knee extension exercise done with Impulse (Newnan, GA). Forty-one women and twenty-nine men made six laboratory visits, including two familiarization sessions of the knee extension exercise and four real workout sessions which entailed four 30-second exercise sets, separated by 120-second rests, against four different loads (0Kg, 3.41Kg, 5.68Kg, 7.95Kg). A Latin Squares design was used to counterbalance the sequence of the sets. Average force (AF), peak force (PF), total work (TW), Impulse value, and the number of knee extension repetitions were each analyzed with a 2 (gender) …

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