Digital Commons Network^™

The Glass Coffin: Gothic Adaptations And The Formation Of Sexual Subjectivity., Colton T. Wilson

Electronic Theses and Dissertations

It is now an almost foregone conclusion that classic depictions of vampirism resonate with contemporary queer audiences. A sympathetic response to the monster’s persecution is often the key factor in these arguments, yet little attention is paid to the textual details that prompt such a process of identification. This study posits that the iconography used to establish a connection between monstrosity and non-normative sexuality has its origins in Victorian Gothic fiction, whose descriptions of vampirism were assimilated into the discourse of the fin-de-siècle medical field known as sexology. Theories that defined homosexuality as an illness with physical and psychological symptoms …

Cannabinoids And Retinal Fibrotic Disorders., Lucy June Sloan

Electronic Theses and Dissertations

Retinal fibrosis is detrimental to vision. Retinal pigment epithelial (RPE) cells contribute to several retinal fibrotic diseases. Upon exposure to TGF-β, a key fibrotic cytokine, RPE cells trans-differentiate to myofibroblasts marked by the integration of α-SMA fibers into F-actin stress fibers, which confer strong contractility. Myofibroblasts produce and contract the collagen-rich fibrotic scar and disrupt retinal architecture. In this study, we investigated the in vitro effects of the putative endocannabinoid compound N-oleoyl dopamine (OLDA) on TGF-β2 induced porcine RPE cell contraction and α-SMA expression. Using an in vitro collagen matrix contraction assay, we found that OLDA inhibited TGF-β2 induced contraction …

Black Women Burdening: The Process Of "Unburdening" Realism In Dennis Mcintyre's Split Second., Brandi L. Threatt

Electronic Theses and Dissertations

Black women who dramatize reality can experience a transference of burdens if realistic plays reflect their lived experiences. Burdens affect truthful character development and impact the mental, emotional, physical, social and spiritual well-being of Black actresses. This thesis will use the Fall 2021 University of Louisville production of Dennis McIntyre’s Split Second as a case study. Gender and Race studies along with an auto-ethnographic research approach will be used to examine how my portrayal of Alea in Split Second contributed to Black Woman Burdening, a phrase I created to examine how Black fatigue can negatively and specifically affect Black women …

It's All Fun And—: A Rhetorical Analysis Of Space In The Pandemic., Erica Von Proctor Lewis

Electronic Theses and Dissertations

This exhibition and document explore spatial rhetoric during the pandemic, utilizing materiality and relational aesthetics to reflect on the different ways in which the public and private are made distinct from one another. In doing so, Lewis addresses new cultural navigations of shared spaces, both digital and corporeal, public and private. In addition, the artist also examines the faulty social and institutional systems that the pandemic brought to light, such as socioeconomic dynamics and voter suppression, while utilizing Kenneth Burke’s concept of the terministic screen. Games are a central theme throughout the exhibition, as they are often coded as “home” …

Forecasting Hypotension By Learning From Multivariate Mixed Responses.., Jodie Ritter

Electronic Theses and Dissertations

Blood Pressure is the main determinant of blood flow to organs. Hypotension is defined as a systolic blood pressure less than 90 mmHg or a diastolic blood pressure less than 50 mmHg. The severity and duration of hypotension is associated with low blood flow to organs often result in organ damage and a high mortality rate. Predicting hypotension prior to surgery and during the surgery can reduce the incidence and duration resulting in better patient outcomes. This thesis uses preoperative bloodwork and vital signs as well as perioperative vital signs in 5-minute increments as inputs to forecast hypotension. Hypotension can …

Dental Morphology In A Juvenile Mammal From The Late Cretaceous Of South America: Tooth Eruption And Development., Kayla E Newton

Electronic Theses and Dissertations

Meridiolestids are a diverse and distinct clade of Cretaceous–Miocene South American dryolestoid mammals ranging in size from small shrew-sized insectivores to large dog- sized omnivores. Cronopio dentiacutus from the Cenomanian La Buitrera locality, Rio Negro Province, Argentina (Candeleros Fm.) is the oldest and best known of them, represented by skull material and associated jaws. A juvenile skull as attributed to Cronopio dentiacutus, based on tooth size and morphology, was collected from the type locality. The specimen includes the skull and both articulated lower jaws. The fossil is poorly ossified and partially flattened; CT scanning reveals most of the dentition …

Fabrication And Characterization Of Lactobacillus Crispatus Containing Bioprints For Bacterial Vaginosis Application., Anthony J. Kyser

Electronic Theses and Dissertations

Bacterial vaginosis (BV) is a condition in which healthy lactobacilli are replaced by an overabundance of pathogenic bacteria in the female reproductive tract. Current antibiotic treatments often fail to “cure” infection, resulting in recurrence in more than 50% of women, 6 months post-treatment. Recently, probiotics have demonstrated promise to restore vaginal health; however, as with other active agents, delivery requires once-to-twice daily administration. Recently, three-dimensional (3D)-bioprinting has enabled the fabrication of well-defined cell-laden architectures with tunable agent release, thereby presenting a novel approach with which to deliver probiotics. One promising bioink, gelatin alginate, was selected for study, due to its …

Release Kinetics Of Metronidazole From 3d Printed Silicone Scaffolds., Sydney E. Herold

Electronic Theses and Dissertations

Sustained local administration of active agents has been proposed to cure bacterial vaginosis in the female reproductive tract and restore the resident bacterial fauna. Bioprinting has shown promise for the development of systems for local agent delivery. In contrast to oral ingestion, agent release kinetics can be fine-tuned by bioprinting specialized scaffold designs tailored for particular treatments while enhancing dosage effectiveness via localized sustained release. It has been challenging to establish scaffold properties for sustained release as a function of fabrication parameters. Towards this goal, we evaluate 3D printed scaffold formulation and feasibility to sustain release of metronidazole, a representative …

Machine Learning Analysis Of Acoustic Attenuation Measurements For Cellular Characterization., John T. Moore

Electronic Theses and Dissertations

T-cell therapies have been gaining popularity in recent years due to their cancer fighting potential. With remission rates improving in this field of immunotherapy, the demand for T-cell therapies has also increased; however, the cell processing techniques for these therapeutic products have yet to rise to the level of demand. The manufacturing process takes too long and a significant amount of processed cell batches can fail to meet safety requirements. These limitations of cell processing can be detrimental to patients seeking T-cell therapies. While current products have improved the time it takes to manufacture these therapeutic products, there is still …

The Influence Of Fraternity And Sorority Characteristics On Alcohol Exposure: Who Is At Risk?, Sydney Shupe

Electronic Theses and Dissertations

Fraternity and sorority membership has been among the highest contributing factors linked to increased exposure to alcohol consumption among college students. Many have argued that this association persists as a result of the drinking sub-culture among Greek organizations, and report that finding effective methods to minimize alcohol consumption among college students is critical. Using self-reported data from fraternity and sorority members at East Tennessee State University (n=107) the link between observed alcohol consumption and demographic characteristics of the individual Greek member and the Greek organization was examined through a quantitative lens. It was hypothesized that the demographic characteristics of the …

Development Of Porous Solid Acid Catalysts For Lignocellulose And Plastic Upcycling., Mohammad Shahinur Rahaman

Electronic Theses and Dissertations

My goal is to develop chemical processes for transforming waste to solve environmental problems and enhance sustainability. Environmental problems such as pollution and massive amounts of waste are the main drivers that stimulate my research ideas. I focused on creating novel, efficient catalytic processes for converting polymeric waste "feedstocks" into high-value chemicals by integrating my expertise in catalysis, materials science, and synthetic chemistry to develop porous solid catalytic materials. During my Ph.D., I focused on two polymeric feedstocks, lignocellulose, and discarded plastic.

Early in my Ph.D. journey, I focused on catalytic upcycling of lignocellulose. Lignocellulosic biomass is cost-effective, abundant, and …

Behavioral Effects And Neurobiological Mechanisms Of 3-Aminobenzimide In A Rodent Model Of Chronic Psychological Stress, Liza Wills

Electronic Theses and Dissertations

Major depressive disorder (MDD) is a leading cause of disability worldwide, with a lifetime prevalence rate of approximately 20%. Inadequate pharmacological treatment methods for MDD are a significant debilitating factor. Patient estimates suggest that the treatment resistance rate for pharmacological interventions is over 30%. Postmortem analyses of human tissue of individuals diagnosed with MDD have shown an increase in Poly (ADP-ribose) polymerase 1 (PARP-1) mRNA gene expression in prefrontal cortical white matter when compared to psychiatrically normal brain tissue. In order to further investigate this issue, the present study used the social defeat stress/chronic unpredictable stress (SDS + CUS) rodent …

The Lived Experiences Of Introverts And Their Extracurricular Involvement In A Four-Year University Environment, Maigan Wipfli

Electronic Theses and Dissertations

This qualitative, phenomenological study explores the lived experiences of introverts who were involved in two or more passive or active extracurricular involvement opportunities during their undergraduate experience at a four-year college or university. Eleven semi-structured interviews were conducted via Zoom video conferencing with self-identified introverts who attended public, four-year universities in the South, West, and Midwest regions of the United States. Participants represented both medium and large institutions. The analysis of the data indicated common themes from the participants including personality traits and common misconceptions of introversion, cultural focus of extraversion, personal and professional development, opportunities granted, and individual preferences …

Perceptions, Knowledge, And Attitudes About Long-Acting Reversible Contraceptives (Larcs) Among Women In Appalachian Tennessee, Fenose Osedeme

Electronic Theses and Dissertations

In Tennessee (TN), the rate of unintended pregnancies remains higher than the national rate (32.4% vs. 30.3%). Although long-acting reversible contraceptives (LARCs) are 99% effective in preventing undesired pregnancies; uptake remains low in rural and underserved communities. Previous research has provided some insight into women’s perceptions of LARCs; however, those guided by conceptual frameworks to understand the multiple influences that impact perceptions towards LARCs, especially among rural regions, are scarce. This multimethod qualitative study explored multiple influences that impact northeast Tennessee women’s perceptions and attitudes toward LARCs using the Socio-ecological Model (SEM) as a guiding framework.

The study comprised six …

Dental Students' Perceptions Of Dental Hygienists' Education And Scope Of Practice, Cynthia Metzger

Electronic Theses and Dissertations

Oral health is strongly correlated to systemic health in the United States. Dental professionals play a significant role in addressing the oral health and prevention of disease for populations in the United States. Because many areas in the United States lack access to dental care, the federal government considers dental hygienists part of the solution. It is now paying attention to restrictive dental hygiene scope of practice rules in each state. State dental boards, comprised mainly of dentists, make these rules based on their perception of dental hygienists' lack of education. This study explored fourth-year dental students' perceptions of the …

Cyberspace Vs Green Space: Nature’S Psychological Influence In Neuromancer, Blade Runner 2049, And The Stone Gods, Zackery Castle

Electronic Theses and Dissertations

Cyberpunk science fiction is often set in dystopian futures where capitalism and rapid technological growth have rendered the planet ecologically devastated. The people of these worlds are host to numerous mental illnesses that many attempt to cure with drugs, entertainment, or other aspects of their fast-paced existences, but these solutions are rarely successful. When characters come to embrace the remnants of the natural world, however, they typically show signs of mental healing as the result of exposure to nature. This thesis analyzes William Gibson’s Neuromancer, Denis Villeneuve’s Blade Runner 2049, and Jeanette Winterson’s The Stone Gods alongside research …

Assessing Best Practices, Perceptions, And Barriers To Breastfeeding In The Appalachian Region, Melissa White

Electronic Theses and Dissertations

Background: Breastfeeding protects against a variety of adverse health outcomes for mothers and babies. Global best practices, known as the Baby-Friendly Hospital Initiative (BFHI), have been developed to support the initiation and exclusivity of breastfeeding during the post-delivery hospital stay. The aims of this study were to explore the literature related to the impact of the BFHI on breastfeeding disparities in the U.S.; compare the impact of exposure to these best practices on exclusive breastfeeding rates in Appalachian and non-Appalachian hospitals; and to understand knowledge, perceptions, and barriers to breastfeeding of postpartum mothers receiving care in a Northeast Tennessee OB/GYN …

Knockdown Of C. Elegans Nad Kinases Nadk-1 Or Nadk-2 Induces An Antioxidant Response Without Affecting Lifespan, Henry Gong

Electronic Theses and Dissertations

Nearly all multicellular organisms show changes in redox balance with aging leading to oxidative damage of macromolecules. This study investigated the role of the [NADP⁺]/[NADPH] redox couple in aging. This redox couple plays an important role in maintaining tissue redox balance and becomes slightly more oxidized in aged tissues. NADPH is a major source of reducing equivalents for enzymes that detoxify hydrogen peroxide. However, catalase detoxifies hydrogen peroxide independently of NADPH. But catalase is absent from mitochondria, a major source of hydrogen peroxide, where instead glutathione plays the major role in hydrogen peroxide detoxification in an NADPH-dependent manner. …

Organizational Communication In Community Colleges: Staff Members’ Perspectives, Sinthea Reynolds

Electronic Theses and Dissertations

The purpose of this research was to determine if there was a significant relationship between scores on the Communication Satisfaction Questionnaire (CSQ) scale of effective managerial communication and scores on the Job Descriptive Index (JDI) scale of job satisfaction for non-faculty staff members at the participating community college.

A total of 75 non-faculty staff members from three separate community colleges in East Tennessee participated in the study. A modified version of the Communication Satisfaction Questionnaire (CSQ) developed by Downs and Hazen (1977) was used to collect data. The instrument used a Likert-type scale with a 7-point scale with eight dimensions …

Junior Reserve Officer Training Corps Instructor Perceptions Of Instructional Delivery In A Virtual Environment, John Derek Faulconer

Electronic Theses and Dissertations

The purpose of this study was to examine the perceptions of Junior Reserve Officer Training Corps (JROTC) instructors at the secondary level as they adapted to teaching in a virtual environment during the COVID-19 global pandemic. Although the literature indicated that the delivery method for the content and curriculum aligned to Junior Reserve Officer Training Corps was best suited for face-to-face instruction, no evidence existed to indicate cadet overall success based on the implementation by virtual delivery, using one or multiple online platforms. The objective of each JROTC Program is to ensure that cadets successfully complete the program with advanced …

Examining How Black Administrator Employment Decisions Are Impacted By The Behaviors Of Supervisors, Annie Wilson Whitaker

Electronic Theses and Dissertations

The purpose of this phenomenological study was to understand how Black, public school administrator employment decisions are impacted by the behaviors of supervisors in Virginia. Commonalities among the lived experiences of Black, public school administrators were examined in order to identify specific behaviors which impact employment decisions.

Transcendental phenomenological qualitative research design was utilized. Participants were identified through gatekeeper introductions and then narrowed through snow-ball sampling. Data was collected through open-ended face-to-face interviews with 12 Black, public school administrators in Virginia via a secure online platform. The data was analyzed in order to identify emergent themes which represent the lived …

Self-Measured Blood Pressure Monitoring In Hypertension Control: The Role Of Social Determinants Of Health, Current State In The United States, And Future Directions, Adekunle Oke

Electronic Theses and Dissertations

Hypertension, a medical condition, predisposes to other cardiovascular diseases, and can be impacted by the social determinants of health (SDOH). Self-measured blood pressure monitoring (SMBP) is an evidence-based approach to hypertension control, but not much is known about the influence of SDOH on SMBP. This dissertation aims to: 1) highlight the SDOH factors whose relationship with SMBP have been explored in research studies; 2) examine the relationship between SDOH and SMBP among United States (U.S.) adults with high blood pressure; and 3) examine the current state of SMBP in the U.S., highlight policy implications from the empirical study and provide …

Hypocretin-Receptor Mrna Expression In The Central Amygdala Of Alcohol-Dependent And Non-Dependent Rats, Gabriel Aldridge

Electronic Theses and Dissertations

Hypocretin/Orexin (HCRT) neurotransmission facilitates drug-seeking behavior. HCRT neurotransmission at HCRT-receptors 1 and 2 (HCRT-R1 and -R2, respectively) is implicated in addiction. During the shift to alcohol-dependency, adaptations in neurotransmitter systems occur in reward- and stress-related brain regions. Specifically, neurotransmission systems in the central amygdala (CeA) are modulated by alcohol drinking/exposure. Therefore, this study investigated Hcrtr1 and Hcrtr2 mRNA expression in the CeA of alcohol-dependent rats and in non-dependent controls during acute alcohol withdrawal. Fos mRNA expression in the CeA of alcohol-dependent and non-dependent rats was also determined to assess adaptations in neuronal activation. To our knowledge, this is the first …

Accepting Or Opposing The Status Quo: A Look At The Women Characters In Mariama Bâ’S So Long A Letter (1981) And Chimamanda Adichie’S Purple Hibiscus (2003), Omolola Giwa

Electronic Theses and Dissertations

What exactly is the status quo of women in Africa? Women’s selfhood has been systematically subordinated or outright denied by law, customary practices, and cultural stereotypes. Scholars like Judith Bennet suggest that religious practices and colonial rule subjugate African women. Patriarchal ideologies guide the society’s discrimination against women and this has influenced the status of women, especially married women and the way they respond in times of affliction.

Authors like Chimamanda Adichie and Mariama Ba in their fictional novels The Purple Hibiscus and So Long a Letter focus on capturing the struggles and conditions of women in the Western African …

Home, Work, Land, Gregory Smith

Electronic Theses and Dissertations

The artist discusses his Master of Fine Arts exhibition, entitled Family, Work, Land. The exhibit was mounted in Tipton Gallery in downtown Johnson City, TN, from February 22nd to March 11th, 2022. A public reception was held on the evening of March 4th . The exhibition consisted principally of four multimedia installations. Smith’s body of work is an interpretation of how stories that he often heard growing up are related to the Western North Carolina community in which his grandparents were living in the first quarter of the twentieth century. These works explore the interactions between people, how they support …

Water Quality Assessment Of Karst Spring Water As A Private Water Supply Source In Northeast Tennessee, Lukman Fashina

Electronic Theses and Dissertations

Karst springs are an essential source of private water supply for about 10% of households in Tennessee. However, these springs, which can be easily polluted, are unregulated. This study, therefore, assesses water quality spatial patterns and water quality rating of roadside springs in northeast Tennessee.

Karst spring water samples collected from 50 springs were assessed using EPA Standard methods for pathogens, nutrients, radon, and physicochemical parameters. Springs generally met federal and state standards for physicochemical parameters, 90% of samples contained E. coli, and all samples contained fecal coliform. High E. coli was spatially clustered causing a fecal contamination hot …

“It Is Not Enough To Be In One Cage With One Self”: The Poetic Subject, Incarceration, And Envisioning Abolition, Emily Price

Electronic Theses and Dissertations

The Beat poet Bob Kaufman was in many ways nearly destroyed by the state. Forcible electroshock therapy, repeated targeting by police, repeated brutalization by police, and frequent homelessness all threatened to snuff him out, but Kaufman refused to give in. He remained a political beacon of hope for his community throughout his life, asking those around him to envision a world where he could be free. Through his poems, through the poems of Etheridge Knight and Jimmy Santiago Baca, and through contemporary visions of abolition from Angela Davis and community organizers that become ever more relevant as the prison system …

Using Macroinvertebrates To Assess The Effects Of Nutrient Input Between The Nolichucky And Pigeon Rivers, Anna Grizzard

Electronic Theses and Dissertations

Previous work found significant differences in growth rates of native mussels at locations downstream from the regulated Walter’s Dam and the out-of-service, free-flowing Davy Crockett Dam. The purpose of this study is to investigate differences within the macroinvertebrate communities related to factors driving the differences in mussel growth between rivers. Macroinvertebrate samples were collected following the Tennessee Department of Environment and Conservation protocol for SQKICK collection and analyzed using the Tennessee Macroinvertebrate Index (TMI). There were no significant differences in TMI scores between the downstream sites of the rivers, but there were significant increases in chlorophyll_a, dissolved oxygen, …

At What Price: Insidious Hegemony And Character Archetypes Woven Into Until Dawn, Courtney Harvey

Electronic Theses and Dissertations

Supermassive Games’s Until Dawn tasks its players with helping eight teenagers survive a night of terror. All eight playable characters may live or die depending on the player’s choices and gameplay proficiency. Despite its intricacies, the game still relies heavily on horror movie tropes, which the characters embody, and they face different treatment based on their gender, race, and sanity. Particularly, the weapons available to them and the scenarios for their deaths and survival contribute to trapping the characters within their given characteristics and forcing them into a role that they cannot ever fully break free from. While the branching …

The Comparative Effects Of Three Virtual Nature Exposure Methods On State Social Motivation: A Randomized Controlled Trial, Samantha A. Castelblanco

Electronic Theses and Dissertations

It is well established in the research literature that exposure to nature positively affects physical and mental health. However, research regarding the effects of nature exposure on social health is less developed. Prior research found that watching a video of nature improved state social motivation. Building on this research, the current study compared the effects of three methods of virtual nature exposure on state social motivation in a randomized controlled trial. In this 3X3 between-subjects experiment, participants (N = 426) 18 to 73 (M_Age = 27.69, SD = 12.19) were randomly assigned to experience 3-minutes of nature …