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Modulation Of Alpha-Subunit Visit-Dg Sequence Residues Ser-347, Gly-351 And Thr-349 In The Catalytic Sites Of Escherichia Coli Atp Synthase., Laura Elaine Brudecki

Electronic Theses and Dissertations

Binding of inorganic phosphate (P_i) in ATP synthase catalytic sites is a crucial step for the synthesis of adenosine-5'-triphosphate (ATP). ATP is the fundamental means of cellular energy in almost every organism, and in order to gain insight into the regulation of ATP catalysis, critical amino acid residues responsible for binding P_i must be identified. Here, we investigate the role of highly conserved α-subunit VISIT-DG sequence residues αSer-347, αGly-351, and αThr-349 in P_i binding. Mutations αS347A/Q, αG351Q, αT349A/D/R, βR182A, and αT349R/βR182A were generated via site directed mutagenesis. Results from biochemical assays showed that αSer-347 is required …

Spatial Analysis Of Motor Vehicle Accidents In Johnson City, Tennessee, As Reported To Washington County Emergency Communications District (911)., Katharine D. Bennett

Electronic Theses and Dissertations

This study spatially analyzes emergency 911 call-for-service records from January 1, 2000 through December 31, 2009 for motor vehicle accidents inside the corporate limits of Johnson City, Tennessee. Records were compared according to the land use classification for adjacent properties, roadway type, and traffic signal proximity. Data were evaluated through ArcGIS software using proximity analysis, point pattern analysis, and hotspot analysis.

Motor vehicle accidents evaluated during this study consist of accident locations reported to the Washington County Emergency Communications District concerning property damage, personal injury, and fatalities. Results indicate localized areas with the highest number of traffic accidents contain the …

An Analysis Of Preschool Enrollment And Student Progress Measures Among Primary And Elementary Students., Charles David Freeman

Electronic Theses and Dissertations

The purpose of this study was to determine if a difference in Dynamic Indicators of Basic Early Literacy Skills (DIBELS) scores exists between students who attended Tennessee's Voluntary Preschool for All program, Head Start, private preschool, or daycare and those who did not attend any type of preschool program for students in grades kindergarten through fifth. The sample consisted of students who attended kindergarten through fifth grade during the 2009-2010 school year in one east Tennessee school system. Data gathered were from Dynamic Indicators of Basic Early Literacy Skills (DIBELS) scores obtained during the 2009-2010 school year and a survey. …

Educational Interventions For Children With Autism Spectrum Disorders: Perceptions Of Parents And Teachers In A Northeast Tennessee School System., Paula Anderson Nickels

Electronic Theses and Dissertations

This qualitative case study investigated the perceptions of parents of children with autism spectrum disorders (ASD), special education teachers, and general education teachers in a Northeast Tennessee public school system regarding educational interventions for children with ASD including what interventions they have found effective, the barriers and challenges they have faced, and how they view their relationships with one another.

Data were collected through interviews with a purposeful sample of 7 parents of 6 children with autism spectrum disorders, 8 special education teachers, and 6 general education teachers and a review of documents. Parent and teacher participants represented children at …

Residential Broadband Access For Students At Walters State Community College., Mark A. Hurst

Electronic Theses and Dissertations

The purpose of this study was to determine the availability of internet access for students attending Walters State Community College during the spring semester 2010. In particular, it is unknown to what degree broadband internet access is available in the counties that Walters State considers the service area of the college.

The research was conducted during the spring semester 2010 including the months February, March, and April of 2010. Data were gathered by surveying currently enrolled students of the college. Twelve percent of the population responded to the study. The survey instrument covered the areas of demographics, Internet connection type …

A Comparison Prior To And After Implementation Of A Ninth Grade Academy In East Tennessee High Schools., Judy Alisa Teffeteller

Electronic Theses and Dissertations

The purpose of this study was to identify student progress during the 9th grade year by evaluating student data prior to and after the implementation of a 9th grade academy in 2 east Tennessee high schools. The testing variables included the number of core credits earned, the number of elective credits earned, number of absences, and grade point average. Grouping variables included all 9th grade students and 9th grade students by gender prior to and after implementation of the 9th grade academy. Data were collected over 5 years (2005-2010). Paired-samples t-tests were used to make comparisons prior to and after …

P300-Based Bci Performance Prediction Through Examination Of Paradigm Manipulations And Principal Components Analysis., Nicholas Edward Schwartz

Electronic Theses and Dissertations

Severe neuromuscular disorders can produce locked-in syndrome (LIS), a loss of nearly all voluntary muscle control. A brain-computer interface (BCI) using the P300 event-related potential provides communication that does not depend on neuromuscular activity and can be useful for those with LIS. Currently, there is no way of determining the effectiveness of P300-based BCIs without testing a person's performance multiple times. Additionally, P300 responses in BCI tasks may not resemble the typical P300 response. I sought to clarify the relationship between the P300 response and BCI task parameters and examine the possibility of a predictive relationship between traditional oddball tasks …

Perceptions Of Maternal Stress And Neonatal Patient Outcomes In A Single Private Room Versus Open Room Neonatal Intensive Care Unit Environment., Lisa M. Smithgall

Electronic Theses and Dissertations

Limited clinical evidence exists regarding whether the single private room Neonatal Intensive Care Unit (NICU) environment of care delivery has a positive, negative, or neutral impact on health outcomes for the high risk neonate and on maternal stress as compared to the open room design. The study purpose was to examine whether a difference exists in health outcomes in the open room versus single private room NICU environment. The factors considered were weight gain, ventilator days, hospital length of stay, incidence and grade of intraventricular hemorrhage (IVH), the number of parental visits, and perceptions of maternal stress. Infants hospitalized in …

The Effect Of Sternocleidomastoid Muscle Activation Pattern And Feedback Condition On The Vestibular Evoked Myogenic Potential., Mary Jo Davenport

Electronic Theses and Dissertations

The vestibular evoked myogenic potential (VEMP) has been shown to be clinically useful in providing diagnostic information regarding the function of the otolith receptors, inferior vestibular nerve, and vestibulospinal pathways. The VEMP is a biphasic response elicited by loud clicks or tone bursts and recorded from the tonically contracted sternocleidomastoid (SCM) muscle. Because the VEMP is an inhibitory response, it is important to investigate stimulus and parameter characteristics in order to determine the optimal test protocol and maximize clinical usefulness. The aims of this study were 1) to evaluate the effects of 4 different methods of SCM muscle activation and …

Mathematical Modeling, Simulation, And Time Series Analysis Of Seasonal Epidemics., Eric Shu Numfor

Electronic Theses and Dissertations

Seasonal and non-seasonal Susceptible-Exposed-Infective-Recovered-Susceptible (SEIRS) models are formulated and analyzed. It is proved that the disease-free steady state of the non-seasonal model is locally asymptotically stable if R_v < 1, and disease invades if R_v > 1. For the seasonal SEIRS model, it is shown that the disease-free periodic solution is locally asymptotically stable when R̅_v < 1, and I(t) is persistent with sustained oscillations when R̅_v > 1. Numerical simulations indicate that the orbit representing I(t) decays when R̅_v < 1 < R_v. The seasonal SEIRS model with routine and pulse vaccination is simulated, and results depict an unsustained decrease …

A Study Of The Effects Of The Length Of Student-Teaching Experiences On New Teacher Efficacy., Alan Wayne Addison

Electronic Theses and Dissertations

This study explores the relationship between the length of student-teaching experiences and new teacher efficacy. Each year thousands of prospective new teachers endure the interview processes to be hired, complete induction programs, and begin their careers only to determine that the teaching profession is not what they assumed it would be. Local school districts spend thousands of dollars each year on orientation for these new teachers only to find them resigning within the first 5 years of service. Increasing new teacher efficacy is imperative to reducing new teacher attrition rates. The more student-teaching experiences an individual collects before entering the …

Discovery And Characterization Of An Antibiotic From The Soil Bacterium Bacillus Sp., Thomas S. Barber

Electronic Theses and Dissertations

Many important antibiotics have become nearly obsolete due to the rise of antibiotic resistant pathogens. Rhodococcus, an actinomycete related to the prolific antibiotic producing genus Streptomyces, harbors over 30 genes for secondary metabolism that could be involved in antibiotic production. Several antibiotics have already been reported for Rhodococcus, suggesting the genus may be a good source for new inhibitory compounds. Fifty four soil bacteria were isolated using enrichment culture techniques (including 37 Rhodococcus) and screened for antibiotic producers. BTHX2, a species of Bacillus was found to have activity against Micrococcus luteus and Rhodococcus erythropolis. BTHX2 …

The Correlation Of The Concentration Of Selected Metals Determined In Water And Fish Samples From A Public Pond., Paula Kay Edwards

Electronic Theses and Dissertations

The concentrations of heavy metals (Cd, Cu, Fe, Ni, Pb, Zn) were measured in water, and fish samples caught from the pond at Fishery Park in Unicoi County, TN. The water samples were collected once a week for 8 weeks. The amounts of metals in the muscle tissues, gill, and liver of the two fish species were measured. This was to determine if any correlation exists between the metal contents in water and those in the fish samples. The concentration ranges for the heavy metals found in the water samples are as follows: Zn 0.04-0.13; Cu, 0.00-0.00; Pb, 0.00-0.59; Cd, …

The Sir Model When S(T) Is A Multi-Exponential Function., Teshome Mogessie Balkew

Electronic Theses and Dissertations

The SIR can be expressed either as a system of nonlinear ordinary differential equations or as a nonlinear Volterra integral equation. In general, neither of these can be solved in closed form. In this thesis, it is shown that if we assume S(t) is a finite multi-exponential, i.e. function of the form S(t) = a+ ∑ⁿ_k=1 r_ke^-σ_kt or a logistic function which is an infinite-multi-exponential, i.e. function of the form S(t) = c + a/b+e^wt, then …

The Role Of Muc1 Splice Variants In Dry Eye And Inflammation., Yoannis Imbert-Fernandez

Electronic Theses and Dissertations

Mucin 1 (MUC1) is a plasma membrane-bound glycoprotein that plays a protective role in corneal epithelial cells. Two full-length splice variants of MUCl: MUCl/B and MUCl/A, that differ by the inclusion of 27 bp from intron 1 and a SNP in MUCl/A, but have identical C-terminal cytoplasmic domain (CD) sequences, were identified in human conjunctival tissue. I tested the hypothesis that MUC1 splice variants are key immunoregulators that act on the ocular surface to protect the ocular surface from inflammation and that their expression correlates with dry eye status. The expression of MUC1/A and MUCl/B splice variant was examined in …

Walking Biomechanics Of End Stage Knee Oa And Tka Patients., Danielle Biton

Electronic Theses and Dissertations

Osteoarthritis (OA) of the knee is associated with decline in functional capacity and ultimately leads to Total Knee Arthroplasty (TKA) in many of these patients. Exercise regimens prior to surgery may potentially enhance pre and post TKA functional performance. However, assessment of such performance should involve biomechanical factors that characterize the mechanisms with which tasks are performed, and not just the quantity of task performed. The present overall study investigated walking biomechanics of end stage knee OA and TKA patients. Throughout the three sub-studies that comprised the overall investigation, particular emphasis was placed on heelstrike and the loading response phase …

Evaluate Experiences Necessary To Achieve Proficiency In Advanced Fiberoptic Intubation Skills : Can We Accelerate The Learning Curve With Simulator Training?, Xinyuan Duan

Electronic Theses and Dissertations

Fiberoptic intubation skills (FOI) are critical in reducing the anesthesia related morbidity and mortality in clinical settings. The purpose of the study was to prove that the simulator can train a novice to achieve the expert level in a relatively short time. The performance of the pre- and post-training of novice group and expert group was computer or video recorded. Three statistical methods were applied for data analysis. The number of airway collisions and the number of passes for oral and nasal were analyzed by a newly proposed maximum likelihood method. The development of this model is based on the …

Data Mining And Analysis Of Lung Cancer Data., Guoxin Tang

Electronic Theses and Dissertations

Lung cancer is the leading cause of cancer death in the United States and the world, with more than 1.3 million deaths worldwide per year. However, because of a lack of effective tools to diagnose Lung Cancer, more than half of all cases are diagnosed at an advanced stage, when surgical resection is unlikely to be feasible. The main purpose of this study is to examine the relationship between patient outcomes and conditions of the patients undergoing different treatments for lung cancer and to develop models to predict the mortality of lung cancer. This study will identify the demographic, finance, …

Determination Of Variables Which Predict Success On The National Council Licensure Examination (Nclex-Pn)., Mary A. Kleber

Electronic Theses and Dissertations

Nursing is one of the most frequently chosen programs by those entering postsecondary institutions in preparation for a career. This is in part due to the job opportunities available, interest in healthcare, and a pending shortage of nurses in healthcare. One strategy for meeting this shortage is the career pathway which encompasses the concept of lifelong learning and provides a mechanism for advancement of nurses along a continuum. Regardless of the level that a student enters the nursing education continuum, failure on the national licensure exam becomes a burden to both graduates and faculty in time and resources expended. Whereas …

Gender Role Stereotypes In Advertising : A Test Of The Match-Up Hypothesis On Advertising Effectiveness., Sara Picklesimer

Electronic Theses and Dissertations

Images in advertisements often depict social expectations for gendered behavior, reinforcing stereotypical masculine and feminine behaviors. This study examined the effectiveness of using gender role stereotypical images in advertisements by matching two products with a male and female, based on the match-up hypothesis. Two gender congruent and two gender incongruent advertisements were created to test the effectiveness of gender congruency on perceived message clarity, advertisement believability, perceived argument quality, attitude toward the advertisement, attitude toward the brand, and purchase intention, based on the information-processing model. Though gender congruent advertisements were perceived as slightly more effective than the gender incongruent advertisements, …

Factors Affecting Nurse Educators' Decision To Teach., Linda J. Thomas 1946-

Electronic Theses and Dissertations

The field of nursing currently faces a shortage of both nurses and nursing faculty. Yet, little is known about why clinical nurses opt to become nursing faculty. Qualitative investigations into this phenomenon are particularly rare. This study addressed these gaps in the context of career choice of faculty associate degree nursing (ADN) programs. The qualitative analysis was guided by Astin's (1984) psychosocial model of career choice. The central research question was, "What factors influence the career choice of nurse educators currently teaching in Kentucky associated degree nursing programs?" Faculty from the 20 ADN programs in public community colleges and 4-year …

School-Based Decision Making (Sbdm) Councils And Their Efficacy And Productivity As Perceived By Council Members., Shawna Shrout Stenton 1976-

Electronic Theses and Dissertations

This study examined the perceptions of Kentucky SBDM council members concerning the productivity and efficacy of the councils. The major variables studied were council member position, council member demographic characteristics, perceived efficacy, and perceived productivity. The research data came from an already completed field survey. This study used the methods of descriptive analysis, causal-comparative analysis, and correlational/predictive research. Statistical procedures included calculation of means and standard deviations, as well as multivariate analysis of variance (MANOVA), ordinary least squares multiple regression, and random effects analysis of variance (ANOVA). The results of the study showed that, in general, council members had a …

How Fares The Selective Tradition? : Racial Representation Of Characters In Second Grade Classroom Libraries., Nancy Franklin Hulan

Electronic Theses and Dissertations

This study investigated teachers' inclusion of racially diverse literature within their classroom libraries, how the representations within the literature compared with the students in the classrooms, the inclusivity of students' book selections, and teacher thoughts concerning selection of literature. Individual classrooms were found to fall within categories of challenging, progressing against, or continuing the selective tradition, the tendency of teachers to favor literature that primarily features White, middle-class, male authors and subjects over works by and about women and other ethnicities or social classes (Luke, Cooke, & Luke, 1986). Of 22 classroom libraries, three were challenging, three were progressing against, …

The Role Of Genetic And Environmental Oxidative Stress Factors In Prostate Cancer., Nicole A. Lavender

Electronic Theses and Dissertations

Prostate cancer (PCA) development may be influenced by genetic variations within oxidative stress response (OSR) related mechanisms, such as antioxidation (e.g., carcinogen metabolism/detoxification), DNA repair, and apoptotic regulation. Excessive oxidative stress can produce DNA base changes, damage tumor suppressors, enhance proto-oncogene expression, and induce malignant transformation of cells. Persistent oxidative stress may even trigger apoptosis. Environmental reactive oxygen species (ROS) exposure attributable to lifestyle factors may exacerbate this situation by increasing oxidative stress. Therefore, it is likely that genetic variation resulting in compromised ROS capacity combined with increased environmental ROS exposure may increase PCA risk and disease aggressiveness. Consequently, this …

Comparative Perspectives Of Direct Support Professionals Working With Adults With Intellectual Disabilities Regarding Standard Competencies., Cynthia Anne Bayes 1955-

Electronic Theses and Dissertations

There is a growing shortage of Direct Support Professionals (DSPs) in the field of intellectual and developmental disabilities. This shortage is at a crisis now and will continue to become more of a crisis unless changes are made. This is a crisis for many community employers and even more of a crisis for the people with disabilities who are faced with constant turnover and the discontinuity created by the withdrawal, gaps in services, and introduction of new people who are likely to leave the position during the first year of employment (Larson & Hewitt, 2005). High turnover, vacancy rates and …

Social Support And Family Criticism: Potential Moderators Of The Relationship Between Functional Impairment, Illness Burden, And Depression., Joshua Paul Hatfield

Electronic Theses and Dissertations

Social support and family criticism variables were examined as potential moderators of the association between illness burden and depression and between functional impairment and depression in a secondary data analysis. Participants (n=735) were older adults aged 65 and older from internal medicine and family medicine primary care offices. It was hypothesized that both illness burden and functional impairment would be associated with increased depressive symptoms and a diagnosis of depression. In addition, it was hypothesized that higher levels of social support and lower levels of family criticism would moderate this relationship. Both linear and logistic multivariate hierarchical regression analyses were …

A Study Of The Perceived Effects Of School Culture On Student Behaviors., Linda Cox Story

Electronic Theses and Dissertations

Research has confirmed that the behaviors of human beings are influenced by their social environments. The school is the principal social environment of adolescents; thus, the school environment necessarily influences the behaviors of students to some degree.

This research project used the interview method to focus on perceptions of school personnel with regard to the elements of school culture that may negatively influence students' behaviors both inside and outside the school environment. The primary influences of school culture on students' behaviors were found to be peers, teachers, administrators, and parent involvlement. Governmental regulations, including those resulting from the Individuals with …

Assurance And Frustration : From Ireland And The Netherlands To Jamestown., Keith A. Donahoe

Electronic Theses and Dissertations

English experience gained from colonization attempts in the New World and in Ireland, as well as military expeditions to the European continent and the New World, helped make Jamestown more successful than any previous English colonial venture in the New World up to that time. Jamestown's early leadership successfully applied the collective English experiences from the Netherlands and Ireland to help the Jamestown colony survive. English experiences in Ireland were instrumental in developing the concepts that led to successful English colonization of North America, but English experiences in the Netherlands also played an important role. In the critical first two-and-a-half …

Tobacco-Induced Changes To Porphyromonas Gingivalis Gene Expression, Phenotype And Host-Pathogen Interactions., Juhi Bagaitkar

Electronic Theses and Dissertations

Tobacco smoke is a strong and independent risk factor for several chronic systemic diseases and also increases susceptibility to a multitude of bacterial infections, including periodontal infections. Periodontitis is a chronic inflammatory disease of the supporting tissues of the periodontium caused by its chief etiological agent, Porphyromonas gingivalis . Smokers are more prone to persistent infections by P. gingivalis , and harbor higher numbers of P. gingivalis than non-smokers. However, smokers show reduced clinical signs of inflammation compared to non-smokers, making diagnosis of periodontal disease in smokers problematic. While several studies delineate the different mechanisms of how tobacco smoke alters …

The Oldham County Learning Institute : Strengthening High School And Middle School Teachers' Abilities To Teach Their Students Powerful Thinking Strategies., Lynda Emery Redmon

Electronic Theses and Dissertations

The Oldham County Learning Institute (OCLI), a 3-day professional development experience for teachers, was provided during four sessions spanning two school years. The OCLI training focused on three interrelated strands: thinking strategies – what to do, gradual release of responsibility – how to do it, and building a classroom community of learners – in what context. This study investigated the impact of OCLI on teachers’ instructional practices with regard to implementation of the three strands. Participants were exposed to seven research-based thinking strategies: a) monitoring for meaning, b) determining importance, c) inferring, d) activating background knowledge, e) visualizing, f) questioning, …